AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047062.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047062.01.T01 MTR_4g120150 99.231 130 1 0 1 130 1 130 1.91e-94 268
MsG0880047062.01.T01 MTR_8g464690 48.837 86 42 1 46 129 5 90 3.25e-26 95.1
MsG0880047062.01.T01 MTR_4g107350 52.439 82 36 2 51 129 220 301 5.71e-21 86.3
MsG0880047062.01.T01 MTR_8g104900 43.478 92 38 2 47 129 39 125 1.52e-18 76.3
MsG0880047062.01.T01 MTR_1g031880 42.045 88 47 1 47 130 53 140 5.12e-18 75.1
MsG0880047062.01.T01 MTR_4g114060 46.591 88 39 3 46 129 155 238 9.07e-18 76.6
MsG0880047062.01.T01 MTR_2g101850 40.698 86 49 1 46 129 5 90 1.18e-16 70.1
MsG0880047062.01.T01 MTR_6g087770 37.647 85 51 1 46 128 5 89 5.19e-16 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047062.01.T01 AT4G15930 66.923 130 36 3 1 130 1 123 8.99e-58 175
MsG0880047062.01.T01 AT4G15930 58.654 104 36 3 1 104 1 97 1.00e-35 119
MsG0880047062.01.T01 AT5G20110 41.463 123 61 3 11 129 86 201 8.10e-20 81.3
MsG0880047062.01.T01 AT1G23220 43.023 86 46 1 47 129 36 121 2.56e-18 75.5
MsG0880047062.01.T01 AT1G52245 40.698 86 49 1 46 129 5 90 1.02e-17 72.8
MsG0880047062.01.T01 AT4G27360 38.824 85 50 1 46 128 5 89 9.49e-17 70.9
MsG0880047062.01.T01 AT3G16120 39.759 83 48 1 49 129 8 90 2.26e-16 69.3
MsG0880047062.01.T01 AT3G16120 39.759 83 48 1 49 129 8 90 2.26e-16 69.3

Find 45 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTTGTTTATTTCTATT+TGG 0.134093 8:+81489845 MsG0880047062.01.T01:CDS
TTCTCCTTGCTGTTGGTTCT+TGG 0.178456 8:-81487267 None:intergenic
TACCTAATTCTCTCTCTTCC+AGG 0.283593 8:-81487328 None:intergenic
GCAAACTGGGTTCTCAAGTT+TGG 0.292328 8:+81490044 MsG0880047062.01.T01:three_prime_UTR
TTCTTCGTTGCGGGTGTTGC+AGG 0.303894 8:-81487472 None:intergenic
CCATTCCATTCACATGTATT+GGG 0.311028 8:+81490111 MsG0880047062.01.T01:three_prime_UTR
ATAATGACCTTCTTCGTTGC+GGG 0.323218 8:-81487481 None:intergenic
TCCATTCCATTCACATGTAT+TGG 0.327294 8:+81490110 MsG0880047062.01.T01:three_prime_UTR
TGTATTGTTGGTCGCAATTT+CGG 0.332097 8:+81487752 MsG0880047062.01.T01:CDS
CACCTGGAAGAGAGAGAATT+AGG 0.341950 8:+81487326 MsG0880047062.01.T01:five_prime_UTR
AGATAATAATTTATGTTGAA+TGG 0.341950 8:-81490166 None:intergenic
ATATCTAATTTCATGCAAAC+TGG 0.351170 8:+81490030 MsG0880047062.01.T01:three_prime_UTR
CCTACTTGGCATTGTATTGT+TGG 0.395219 8:+81487740 MsG0880047062.01.T01:CDS
AGGTGGAGGAGCAGCTCTAA+TGG 0.440034 8:+81487387 MsG0880047062.01.T01:CDS
ACAAGAGGCATGGTCCTACT+TGG 0.447517 8:+81487726 MsG0880047062.01.T01:CDS
AAGAAGCACAGCGCAGGTGG+AGG 0.466246 8:+81487373 MsG0880047062.01.T01:CDS
GCGAAGAAGCACAGCGCAGG+TGG 0.479599 8:+81487370 MsG0880047062.01.T01:CDS
AATTAGATTCTCCTTGCTGT+TGG 0.495644 8:-81487274 None:intergenic
GAATTCCCAATACATGTGAA+TGG 0.495884 8:-81490116 None:intergenic
GAGGCGAAGAAGCACAGCGC+AGG 0.496399 8:+81487367 MsG0880047062.01.T01:CDS
TTGTAAAGAGTTTGTGCTAG+TGG 0.509356 8:-81489990 None:intergenic
TGCAACACCCGCAACGAAGA+AGG 0.520232 8:+81487474 MsG0880047062.01.T01:CDS
TTGCATGTCAGGGAGCATAT+CGG 0.526660 8:-81487512 None:intergenic
TGTTGCAGAGACTATAAAGA+AGG 0.528953 8:+81487697 MsG0880047062.01.T01:CDS
CCCAATACATGTGAATGGAA+TGG 0.535113 8:-81490111 None:intergenic
TGAATGGAATGGAACACAAA+GGG 0.537376 8:-81490100 None:intergenic
GTTTGTTTCATGAGTCACAT+AGG 0.542936 8:-81489823 None:intergenic
TGCGGGTGTTGCAGGAGAAG+CGG 0.546280 8:-81487464 None:intergenic
TCTGGCTAGCCTTATTCTGA+TGG 0.546660 8:+81489894 MsG0880047062.01.T01:exon
TATGCTCCCTGACATGCAAA+AGG 0.547026 8:+81487516 MsG0880047062.01.T01:CDS
TATCTAATTTCATGCAAACT+GGG 0.565835 8:+81490031 MsG0880047062.01.T01:three_prime_UTR
GATAATGACCTTCTTCGTTG+CGG 0.566862 8:-81487482 None:intergenic
GGAGAAGCGGATGCAGAGAG+TGG 0.576599 8:-81487451 None:intergenic
CCAAATAGAAATAAACAAAG+TGG 0.592216 8:-81489845 None:intergenic
CCAACAATACAATGCCAAGT+AGG 0.596782 8:-81487740 None:intergenic
AATTGAATTAGGCATTTGAG+AGG 0.599018 8:+81487657 MsG0880047062.01.T01:intron
GGCTAGCCTTATTCTGATGG+AGG 0.621751 8:+81489897 MsG0880047062.01.T01:exon
TAGGTAAAGAAAGATGAGCG+AGG 0.624160 8:+81487345 MsG0880047062.01.T01:exon
AAGGAGTTTGACAAGAGGCA+TGG 0.631606 8:+81487716 MsG0880047062.01.T01:CDS
AAATCCAAGAACCAACAGCA+AGG 0.645600 8:+81487263 MsG0880047062.01.T01:five_prime_UTR
GTGAATGGAATGGAACACAA+AGG 0.653029 8:-81490101 None:intergenic
GAGAGTGGAGATGTAACAAG+TGG 0.663410 8:-81487436 None:intergenic
AACAACGACAGCGATATCAA+CGG 0.685652 8:-81487542 None:intergenic
TAAAGAAGGAGTTTGACAAG+AGG 0.686443 8:+81487711 MsG0880047062.01.T01:CDS
GTAAAGAAAGATGAGCGAGG+AGG 0.700815 8:+81487348 MsG0880047062.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAAAAAATTAAATTCTA+AGG + Chr8:81487999-81488018 MsG0880047062.01.T01:intron 10.0%
!! AAATTAGAAAAAATTGAAGA+AGG - Chr8:81487602-81487621 None:intergenic 15.0%
!! AAATTTGCATTAAAACTTTA+TGG - Chr8:81489565-81489584 None:intergenic 15.0%
!! ATAGATTATAGATCTAATTA+AGG - Chr8:81487791-81487810 None:intergenic 15.0%
!! CCATATAGAATTATATTATT+AGG - Chr8:81489727-81489746 None:intergenic 15.0%
!! CCTAATAATATAATTCTATA+TGG + Chr8:81489724-81489743 MsG0880047062.01.T01:intron 15.0%
!! GAAAGATATAAATATTTGAT+GGG + Chr8:81489442-81489461 MsG0880047062.01.T01:intron 15.0%
!! TAGATTATAGATCTAATTAA+GGG - Chr8:81487790-81487809 None:intergenic 15.0%
!! TATAAGTTTGATATAATGAA+CGG + Chr8:81489110-81489129 MsG0880047062.01.T01:intron 15.0%
!! TGAAAGATATAAATATTTGA+TGG + Chr8:81489441-81489460 MsG0880047062.01.T01:intron 15.0%
!!! CTAACAACAATTTTAAAAAA+TGG - Chr8:81488061-81488080 None:intergenic 15.0%
!!! TATTGTTTTGAAATTGAATT+AGG + Chr8:81487646-81487665 MsG0880047062.01.T01:intron 15.0%
!!! TTTTTTTTTTTTTTGTGATG+TGG + Chr8:81488919-81488938 MsG0880047062.01.T01:intron 15.0%
!! AAAGTGATCTTATACATAAA+TGG - Chr8:81489631-81489650 None:intergenic 20.0%
!! AGATTATAGATCTAATTAAG+GGG - Chr8:81487789-81487808 None:intergenic 20.0%
!! ATCAAAAGAAAACATATGTT+TGG - Chr8:81489374-81489393 None:intergenic 20.0%
!! ATTCTCATAAAGATAGAATT+GGG - Chr8:81488579-81488598 None:intergenic 20.0%
!! GTAGTAAATAGAAAAAATCT+AGG - Chr8:81487919-81487938 None:intergenic 20.0%
!! TATTCTCATAAAGATAGAAT+TGG - Chr8:81488580-81488599 None:intergenic 20.0%
!! TGACTTATTCATAATACAAT+CGG - Chr8:81489159-81489178 None:intergenic 20.0%
!!! AGTATTTAGTTTGTTAACTT+TGG + Chr8:81488384-81488403 MsG0880047062.01.T01:intron 20.0%
!!! ATATTACTTCTAGAAAAGAA+GGG + Chr8:81489303-81489322 MsG0880047062.01.T01:intron 20.0%
!!! GTGTTTTATAGATTGAATTT+TGG + Chr8:81488119-81488138 MsG0880047062.01.T01:intron 20.0%
!!! TATATTACTTCTAGAAAAGA+AGG + Chr8:81489302-81489321 MsG0880047062.01.T01:intron 20.0%
! AACATTTACACAATTTAGCA+TGG - Chr8:81488618-81488637 None:intergenic 25.0%
! ACACAATTCAATCATTAACT+AGG - Chr8:81489774-81489793 None:intergenic 25.0%
! ATATCTAATTTCATGCAAAC+TGG + Chr8:81490030-81490049 MsG0880047062.01.T01:three_prime_UTR 25.0%
! ATTATATTATTAGGCTTAGC+AGG - Chr8:81489718-81489737 None:intergenic 25.0%
! CAATCTATAAAACACATACA+CGG - Chr8:81488114-81488133 None:intergenic 25.0%
! CATAACTATGAGTTAGAAAT+TGG - Chr8:81487886-81487905 None:intergenic 25.0%
! CCAAATAGAAATAAACAAAG+TGG - Chr8:81489848-81489867 None:intergenic 25.0%
! TAATGATTGAATTGTGTTCA+TGG + Chr8:81489777-81489796 MsG0880047062.01.T01:intron 25.0%
! TATCTAATTTCATGCAAACT+GGG + Chr8:81490031-81490050 MsG0880047062.01.T01:three_prime_UTR 25.0%
!! TTACTACTGTTTTCTTATTG+TGG + Chr8:81487932-81487951 MsG0880047062.01.T01:intron 25.0%
!! TTATGTTTTCTTCTCTCATA+TGG + Chr8:81488696-81488715 MsG0880047062.01.T01:intron 25.0%
!!! AAATACTGTTTTTTCACTGA+GGG - Chr8:81488371-81488390 None:intergenic 25.0%
!!! AAATATTTGATGGGTTTGAT+AGG + Chr8:81489451-81489470 MsG0880047062.01.T01:intron 25.0%
!!! AATATTTGATGGGTTTGATA+GGG + Chr8:81489452-81489471 MsG0880047062.01.T01:intron 25.0%
!!! CCACTTTGTTTATTTCTATT+TGG + Chr8:81489845-81489864 MsG0880047062.01.T01:CDS 25.0%
!!! TAAATACTGTTTTTTCACTG+AGG - Chr8:81488372-81488391 None:intergenic 25.0%
!!! TTAGTTTGTTAACTTTGGAT+CGG + Chr8:81488389-81488408 MsG0880047062.01.T01:intron 25.0%
!!! TTTTTTTTGTGATGTGGAAA+AGG + Chr8:81488925-81488944 MsG0880047062.01.T01:intron 25.0%
AAACAGGTATGTCGTTAAAT+TGG - Chr8:81488342-81488361 None:intergenic 30.0%
AAAGAACACAGAATACAAAG+TGG - Chr8:81488257-81488276 None:intergenic 30.0%
AACACTATTAAGAGTTCTTC+AGG - Chr8:81488460-81488479 None:intergenic 30.0%
AATTGAATTAGGCATTTGAG+AGG + Chr8:81487657-81487676 MsG0880047062.01.T01:intron 30.0%
AATTTAACGACATACCTGTT+TGG + Chr8:81488341-81488360 MsG0880047062.01.T01:intron 30.0%
ATCTTTCATTCTACACTTCA+TGG - Chr8:81489429-81489448 None:intergenic 30.0%
CATTAAAACTTTATGGCTTC+TGG - Chr8:81489558-81489577 None:intergenic 30.0%
TGTGTAGTTAACAAAATGGA+AGG + Chr8:81487955-81487974 MsG0880047062.01.T01:intron 30.0%
! CTGTGAACAGAAAAATGTTT+TGG - Chr8:81488514-81488533 None:intergenic 30.0%
! GCATGATATTTGTGATGTTT+TGG - Chr8:81489609-81489628 None:intergenic 30.0%
! GGAAATTTCTTTCTTTTTGC+AGG + Chr8:81489798-81489817 MsG0880047062.01.T01:intron 30.0%
!!! AATACTGTTTTTTCACTGAG+GGG - Chr8:81488370-81488389 None:intergenic 30.0%
AAGATGTTGGATGTCGAAAA+GGG - Chr8:81488550-81488569 None:intergenic 35.0%
AGATGAGTAATTCTTGAGTG+GGG + Chr8:81488174-81488193 MsG0880047062.01.T01:intron 35.0%
CAAATGAGGATATGATTGTC+TGG + Chr8:81489691-81489710 MsG0880047062.01.T01:intron 35.0%
CAATTGCCATAAATTCCCTA+AGG - Chr8:81489402-81489421 None:intergenic 35.0%
CCATTCCATTCACATGTATT+GGG + Chr8:81490111-81490130 MsG0880047062.01.T01:three_prime_UTR 35.0%
GAATTCCCAATACATGTGAA+TGG - Chr8:81490119-81490138 None:intergenic 35.0%
GACACACACAAAATGTATTG+AGG - Chr8:81489208-81489227 None:intergenic 35.0%
GAGATGAGTAATTCTTGAGT+GGG + Chr8:81488173-81488192 MsG0880047062.01.T01:intron 35.0%
GTGGTGTGTAGTTAACAAAA+TGG + Chr8:81487951-81487970 MsG0880047062.01.T01:intron 35.0%
GTTCCAGTTTCAAAATACCA+GGG - Chr8:81489244-81489263 None:intergenic 35.0%
GTTTGTTTCATGAGTCACAT+AGG - Chr8:81489826-81489845 None:intergenic 35.0%
TAAAGAAGGAGTTTGACAAG+AGG + Chr8:81487711-81487730 MsG0880047062.01.T01:CDS 35.0%
TCAGACTGCTATATCAAATG+AGG + Chr8:81489677-81489696 MsG0880047062.01.T01:intron 35.0%
TCCATTCCATTCACATGTAT+TGG + Chr8:81490110-81490129 MsG0880047062.01.T01:three_prime_UTR 35.0%
TGAAGTCTAATACATCTGAG+AGG + Chr8:81488826-81488845 MsG0880047062.01.T01:intron 35.0%
TGAATGGAATGGAACACAAA+GGG - Chr8:81490103-81490122 None:intergenic 35.0%
TGTATTCTGTGTTCTTTGCA+GGG + Chr8:81488260-81488279 MsG0880047062.01.T01:intron 35.0%
TGTATTGTTGGTCGCAATTT+CGG + Chr8:81487752-81487771 MsG0880047062.01.T01:CDS 35.0%
TGTTGCAGAGACTATAAAGA+AGG + Chr8:81487697-81487716 MsG0880047062.01.T01:CDS 35.0%
TTGGAATGATAAACTGACAC+AGG - Chr8:81488323-81488342 None:intergenic 35.0%
TTGTAAAGAGTTTGTGCTAG+TGG - Chr8:81489993-81490012 None:intergenic 35.0%
TTGTATTCTGTGTTCTTTGC+AGG + Chr8:81488259-81488278 MsG0880047062.01.T01:intron 35.0%
! AACAAGTGGTTTTCGATCTT+CGG - Chr8:81487425-81487444 None:intergenic 35.0%
! AATTAGATTCTCCTTGCTGT+TGG - Chr8:81487277-81487296 None:intergenic 35.0%
! CAGTAAATAACAAAGTGCCA+CGG - Chr8:81488671-81488690 None:intergenic 35.0%
! TTCTAGAAAAGAAGGGTCAA+GGG + Chr8:81489310-81489329 MsG0880047062.01.T01:intron 35.0%
! TTCTTTTGATGTCAGCCTTA+GGG + Chr8:81489384-81489403 MsG0880047062.01.T01:intron 35.0%
! TTTCTTTTGATGTCAGCCTT+AGG + Chr8:81489383-81489402 MsG0880047062.01.T01:intron 35.0%
! TTTTTGCCTCCATCAGAATA+AGG - Chr8:81489906-81489925 None:intergenic 35.0%
!! ATTAAATTCTAAGGCGTGTC+TGG + Chr8:81488008-81488027 MsG0880047062.01.T01:intron 35.0%
!! TTTCAAGTTGAGTTAATCCG+TGG + Chr8:81488651-81488670 MsG0880047062.01.T01:intron 35.0%
!!! CTGGTATTTTGAAACTGGAA+CGG + Chr8:81489243-81489262 MsG0880047062.01.T01:intron 35.0%
!!! CTTTTCGAGTTATTTCTACC+TGG + Chr8:81488151-81488170 MsG0880047062.01.T01:intron 35.0%
AAATCCAAGAACCAACAGCA+AGG + Chr8:81487263-81487282 MsG0880047062.01.T01:five_prime_UTR 40.0%
AACAACGACAGCGATATCAA+CGG - Chr8:81487545-81487564 None:intergenic 40.0%
AATTGGGAACAGCAAGATGT+TGG - Chr8:81488563-81488582 None:intergenic 40.0%
ACACAATTTAGCATGGCGTT+TGG - Chr8:81488611-81488630 None:intergenic 40.0%
AGTCTAATACATCTGAGAGG+AGG + Chr8:81488829-81488848 MsG0880047062.01.T01:intron 40.0%
ATAATGACCTTCTTCGTTGC+GGG - Chr8:81487484-81487503 None:intergenic 40.0%
ATCAAAGCTTGGACTAAGCT+CGG + Chr8:81489509-81489528 MsG0880047062.01.T01:intron 40.0%
CAAGATGTTGGATGTCGAAA+AGG - Chr8:81488551-81488570 None:intergenic 40.0%
CCAACAATACAATGCCAAGT+AGG - Chr8:81487743-81487762 None:intergenic 40.0%
CCCAATACATGTGAATGGAA+TGG - Chr8:81490114-81490133 None:intergenic 40.0%
CCTACTTGGCATTGTATTGT+TGG + Chr8:81487740-81487759 MsG0880047062.01.T01:CDS 40.0%
CGTTCCAGTTTCAAAATACC+AGG - Chr8:81489245-81489264 None:intergenic 40.0%
CTCAAGAATTACTCATCTCC+AGG - Chr8:81488172-81488191 None:intergenic 40.0%
GAAACTGGAACGGCTAATAA+CGG + Chr8:81489253-81489272 MsG0880047062.01.T01:intron 40.0%
GACAGGAGTAGAAATCACAT+AGG - Chr8:81489045-81489064 None:intergenic 40.0%
GAGTTTGATCGTAGTGGTAT+AGG + Chr8:81488230-81488249 MsG0880047062.01.T01:intron 40.0%
GATAATGACCTTCTTCGTTG+CGG - Chr8:81487485-81487504 None:intergenic 40.0%
GGAGATGAGTAATTCTTGAG+TGG + Chr8:81488172-81488191 MsG0880047062.01.T01:intron 40.0%
GGGATAGAATAGGCTTGATA+GGG + Chr8:81488429-81488448 MsG0880047062.01.T01:intron 40.0%
GTATTCTGTGTTCTTTGCAG+GGG + Chr8:81488261-81488280 MsG0880047062.01.T01:intron 40.0%
GTCTAATACATCTGAGAGGA+GGG + Chr8:81488830-81488849 MsG0880047062.01.T01:intron 40.0%
GTGAATGGAATGGAACACAA+AGG - Chr8:81490104-81490123 None:intergenic 40.0%
GTTAAGATGTGCAGAATGAC+AGG - Chr8:81489062-81489081 None:intergenic 40.0%
TACCTAATTCTCTCTCTTCC+AGG - Chr8:81487331-81487350 None:intergenic 40.0%
TAGGTAAAGAAAGATGAGCG+AGG + Chr8:81487345-81487364 MsG0880047062.01.T01:exon 40.0%
TATTCTGTGTTCTTTGCAGG+GGG + Chr8:81488262-81488281 MsG0880047062.01.T01:intron 40.0%
TGGGATAGAATAGGCTTGAT+AGG + Chr8:81488428-81488447 MsG0880047062.01.T01:intron 40.0%
TGTCAGCCTTAGGGAATTTA+TGG + Chr8:81489393-81489412 MsG0880047062.01.T01:intron 40.0%
! AATTCTTGAGTGGGGTACTT+AGG + Chr8:81488182-81488201 MsG0880047062.01.T01:intron 40.0%
! AATTTGAGACTCAAGCAGTG+CGG - Chr8:81489011-81489030 None:intergenic 40.0%
! ATTCTTGAGTGGGGTACTTA+GGG + Chr8:81488183-81488202 MsG0880047062.01.T01:intron 40.0%
! CTTCTAGAAAAGAAGGGTCA+AGG + Chr8:81489309-81489328 MsG0880047062.01.T01:intron 40.0%
!! TTTGATGGGTTTGATAGGGA+TGG + Chr8:81489456-81489475 MsG0880047062.01.T01:intron 40.0%
!!! AAGCTTAAGTCGCTTTTGCA+TGG - Chr8:81489084-81489103 None:intergenic 40.0%
!!! GCAGTTTTGCTTTTCAAGTC+TGG + Chr8:81489876-81489895 MsG0880047062.01.T01:CDS 40.0%
AAGGAGTTTGACAAGAGGCA+TGG + Chr8:81487716-81487735 MsG0880047062.01.T01:CDS 45.0%
CACCTGGAAGAGAGAGAATT+AGG + Chr8:81487326-81487345 MsG0880047062.01.T01:five_prime_UTR 45.0%
CCAACTGAGTTTGATCGTAG+TGG + Chr8:81488224-81488243 MsG0880047062.01.T01:intron 45.0%
CCACTACGATCAAACTCAGT+TGG - Chr8:81488227-81488246 None:intergenic 45.0%
CTATTCCGTGGAAGAGGAAA+AGG - Chr8:81488297-81488316 None:intergenic 45.0%
GAGAGTGGAGATGTAACAAG+TGG - Chr8:81487439-81487458 None:intergenic 45.0%
GCAAACTGGGTTCTCAAGTT+TGG + Chr8:81490044-81490063 MsG0880047062.01.T01:three_prime_UTR 45.0%
GTAAAGAAAGATGAGCGAGG+AGG + Chr8:81487348-81487367 MsG0880047062.01.T01:exon 45.0%
GTGTGTGCTTGGGATAGAAT+AGG + Chr8:81488419-81488438 MsG0880047062.01.T01:intron 45.0%
TATGCTCCCTGACATGCAAA+AGG + Chr8:81487516-81487535 MsG0880047062.01.T01:CDS 45.0%
TCAAAGCTTGGACTAAGCTC+GGG + Chr8:81489510-81489529 MsG0880047062.01.T01:intron 45.0%
TTAGCATGGCGTTTGGACTA+TGG - Chr8:81488604-81488623 None:intergenic 45.0%
! AGTCCAAGCTTTGATTGTGC+TGG - Chr8:81489504-81489523 None:intergenic 45.0%
! ATTTGAGACTCAAGCAGTGC+GGG - Chr8:81489010-81489029 None:intergenic 45.0%
! CGGAGTTGTTTGCTGAACTT+AGG + Chr8:81489130-81489149 MsG0880047062.01.T01:intron 45.0%
! GTCCAAGCTTTGATTGTGCT+GGG - Chr8:81489503-81489522 None:intergenic 45.0%
! TGGTTTTCGATCTTCGGTGA+GGG - Chr8:81487419-81487438 None:intergenic 45.0%
! TTGCATGTCAGGGAGCATAT+CGG - Chr8:81487515-81487534 None:intergenic 45.0%
!! TCTGGCTAGCCTTATTCTGA+TGG + Chr8:81489894-81489913 MsG0880047062.01.T01:exon 45.0%
!! TTCTCCTTGCTGTTGGTTCT+TGG - Chr8:81487270-81487289 None:intergenic 45.0%
!!! GCTCCCTGGTATTTTGAAAC+TGG + Chr8:81489238-81489257 MsG0880047062.01.T01:intron 45.0%
!! AAAAAAAAAAAATAGTATAT+TGG - Chr8:81488908-81488927 None:intergenic 5.0%
AGCCCAGCACAATCAAAGCT+TGG + Chr8:81489498-81489517 MsG0880047062.01.T01:intron 50.0%
AGCTCGGGTTTCAGATACCA+TGG + Chr8:81489525-81489544 MsG0880047062.01.T01:intron 50.0%
CGGATTGAGTGTGTGTGCTT+GGG + Chr8:81488409-81488428 MsG0880047062.01.T01:intron 50.0%
GTTCTGCTCAGCAATATCGC+AGG - Chr8:81488782-81488801 None:intergenic 50.0%
TAGGGATGGAGAGACTGCAA+AGG + Chr8:81489470-81489489 MsG0880047062.01.T01:intron 50.0%
TCAAAATACCAGGGAGCCAG+AGG - Chr8:81489235-81489254 None:intergenic 50.0%
TCACTGAGGGGAAACCAAAC+AGG - Chr8:81488358-81488377 None:intergenic 50.0%
TCGGATTGAGTGTGTGTGCT+TGG + Chr8:81488408-81488427 MsG0880047062.01.T01:intron 50.0%
TTTGTGTGTGTCGAGTCCTC+TGG + Chr8:81489216-81489235 MsG0880047062.01.T01:intron 50.0%
! ACAAGAGGCATGGTCCTACT+TGG + Chr8:81487726-81487745 MsG0880047062.01.T01:CDS 50.0%
! ACGGCTTCCTTTTGCATGTC+AGG - Chr8:81487526-81487545 None:intergenic 50.0%
! AGGGAGTGTGATCTCTGGTA+TGG + Chr8:81489329-81489348 MsG0880047062.01.T01:intron 50.0%
! CGGCTTCCTTTTGCATGTCA+GGG - Chr8:81487525-81487544 None:intergenic 50.0%
! GGGGTACTTAGGGATGACAA+TGG + Chr8:81488193-81488212 MsG0880047062.01.T01:intron 50.0%
! GGTGTCCTTTTCCTCTTCCA+CGG + Chr8:81488289-81488308 MsG0880047062.01.T01:intron 50.0%
! GTGGTTTTCGATCTTCGGTG+AGG - Chr8:81487420-81487439 None:intergenic 50.0%
! GTGTTCTTTGCAGGGGGTTT+TGG + Chr8:81488268-81488287 MsG0880047062.01.T01:intron 50.0%
!! GGCTAGCCTTATTCTGATGG+AGG + Chr8:81489897-81489916 MsG0880047062.01.T01:exon 50.0%
!! TGGCTTCTGGAACATCACCA+TGG - Chr8:81489545-81489564 None:intergenic 50.0%
AGGCAGCTATTCCGTGGAAG+AGG - Chr8:81488303-81488322 None:intergenic 55.0%
CACACACACACACAGTCACC+TGG + Chr8:81487310-81487329 MsG0880047062.01.T01:five_prime_UTR 55.0%
CAGGTGACTGTGTGTGTGTG+TGG - Chr8:81487312-81487331 None:intergenic 55.0%
TGACACAGGCAGCTATTCCG+TGG - Chr8:81488309-81488328 None:intergenic 55.0%
TGCAACACCCGCAACGAAGA+AGG + Chr8:81487474-81487493 MsG0880047062.01.T01:CDS 55.0%
! AGGTGGAGGAGCAGCTCTAA+TGG + Chr8:81487387-81487406 MsG0880047062.01.T01:CDS 55.0%
! GGTCAAGGGAGTGTGATCTC+TGG + Chr8:81489324-81489343 MsG0880047062.01.T01:intron 55.0%
! TTCTTCGTTGCGGGTGTTGC+AGG - Chr8:81487475-81487494 None:intergenic 55.0%
GGAGAAGCGGATGCAGAGAG+TGG - Chr8:81487454-81487473 None:intergenic 60.0%
! AAGAAGCACAGCGCAGGTGG+AGG + Chr8:81487373-81487392 MsG0880047062.01.T01:CDS 60.0%
! TGCGGGTGTTGCAGGAGAAG+CGG - Chr8:81487467-81487486 None:intergenic 60.0%
! GAGGCGAAGAAGCACAGCGC+AGG + Chr8:81487367-81487386 MsG0880047062.01.T01:CDS 65.0%
! GCGAAGAAGCACAGCGCAGG+TGG + Chr8:81487370-81487389 MsG0880047062.01.T01:CDS 65.0%
! TGTCGAGTCCTCTGGCTCCC+TGG + Chr8:81489224-81489243 MsG0880047062.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 81487257 81490173 81487257 ID=MsG0880047062.01;Name=MsG0880047062.01
Chr8 mRNA 81487257 81490173 81487257 ID=MsG0880047062.01.T01;Parent=MsG0880047062.01;Name=MsG0880047062.01.T01;_AED=0.49;_eAED=0.49;_QI=101|1|1|1|1|1|3|271|130
Chr8 exon 81487257 81487561 81487257 ID=MsG0880047062.01.T01:exon:34924;Parent=MsG0880047062.01.T01
Chr8 exon 81487668 81487773 81487668 ID=MsG0880047062.01.T01:exon:34925;Parent=MsG0880047062.01.T01
Chr8 exon 81489820 81490173 81489820 ID=MsG0880047062.01.T01:exon:34926;Parent=MsG0880047062.01.T01
Chr8 five_prime_UTR 81487257 81487357 81487257 ID=MsG0880047062.01.T01:five_prime_utr;Parent=MsG0880047062.01.T01
Chr8 CDS 81487358 81487561 81487358 ID=MsG0880047062.01.T01:cds;Parent=MsG0880047062.01.T01
Chr8 CDS 81487668 81487773 81487668 ID=MsG0880047062.01.T01:cds;Parent=MsG0880047062.01.T01
Chr8 CDS 81489820 81489902 81489820 ID=MsG0880047062.01.T01:cds;Parent=MsG0880047062.01.T01
Chr8 three_prime_UTR 81489903 81490173 81489903 ID=MsG0880047062.01.T01:three_prime_utr;Parent=MsG0880047062.01.T01
Gene Sequence

>MsG0880047062.01.T01

ATGAGCGAGGAGGCGAAGAAGCACAGCGCAGGTGGAGGAGCAGCTCTAATGGCGAAATCCCTCACCGAAGATCGAAAACCACTTGTTACATCTCCACTCTCTGCATCCGCTTCTCCTGCAACACCCGCAACGAAGAAGGTCATTATCAAGAGTGCCGATATGCTCCCTGACATGCAAAAGGAAGCCGTTGATATCGCTGTCGTTGCATTTGAGAGGCACAGCGTAGAGAAAGATGTTGCAGAGACTATAAAGAAGGAGTTTGACAAGAGGCATGGTCCTACTTGGCATTGTATTGTTGGTCGCAATTTCGGTTCCTATGTGACTCATGAAACAAACCACTTTGTTTATTTCTATTTGGATCAGAAAGCAGTTTTGCTTTTCAAGTCTGGCTAG
Protein sequence

>MsG0880047062.01.T01

MSEEAKKHSAGGGAALMAKSLTEDRKPLVTSPLSASASPATPATKKVIIKSADMLPDMQKEAVDIAVVAFERHSVEKDVAETIKKEFDKRHGPTWHCIVGRNFGSYVTHETNHFVYFYLDQKAVLLFKSG*