Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044564.01.T01 | XP_013445572.1 | 99.083 | 109 | 1 | 0 | 6 | 114 | 1 | 109 | 2.61E-73 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044564.01.T01 | O96860 | 55.556 | 90 | 38 | 1 | 6 | 95 | 1 | 88 | 2.28E-29 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044564.01.T01 | A0A072TRW8 | 99.083 | 109 | 1 | 0 | 6 | 114 | 1 | 109 | 1.25e-73 | 223 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0880044564.01 | 0.804443 | 2.154485e-49 | 1.110987e-46 |
| MsG0080048802.01 | MsG0880044564.01 | 0.818624 | 1.779086e-52 | 1.338486e-49 |
| MsG0080049040.01 | MsG0880044564.01 | 0.807030 | 6.167974e-50 | 3.399253e-47 |
| MsG0080049068.01 | MsG0880044564.01 | 0.820673 | 6.057605e-53 | 4.824060e-50 |
| MsG0080049069.01 | MsG0880044564.01 | 0.826965 | 2.030890e-54 | 1.933801e-51 |
| MsG0180000246.01 | MsG0880044564.01 | 0.840336 | 9.245371e-58 | 1.312691e-54 |
| MsG0180001270.01 | MsG0880044564.01 | 0.827221 | 1.763249e-54 | 1.691522e-51 |
| MsG0180002700.01 | MsG0880044564.01 | 0.816655 | 4.945295e-52 | 3.525103e-49 |
| MsG0880044523.01 | MsG0880044564.01 | 0.859776 | 3.272935e-63 | 8.745092e-60 |
| MsG0880044524.01 | MsG0880044564.01 | 0.934937 | 1.857878e-96 | 1.451933e-91 |
| MsG0880044564.01 | MsG0880047152.01 | 0.807909 | 4.014083e-50 | 2.263276e-47 |
| MsG0880044564.01 | MsG0880047451.01 | 0.820820 | 5.603919e-53 | 4.481455e-50 |
| MsG0180004448.01 | MsG0880044564.01 | 0.818979 | 1.477017e-52 | 1.122405e-49 |
| MsG0180005184.01 | MsG0880044564.01 | 0.807725 | 4.393736e-50 | 2.465579e-47 |
| MsG0180005339.01 | MsG0880044564.01 | 0.803073 | 4.148068e-49 | 2.064943e-46 |
| MsG0180005391.01 | MsG0880044564.01 | 0.826159 | 3.161481e-54 | 2.941366e-51 |
| MsG0180005418.01 | MsG0880044564.01 | 0.829777 | 4.256648e-55 | 4.400554e-52 |
| MsG0780041159.01 | MsG0880044564.01 | 0.801212 | 1.001638e-48 | 4.756293e-46 |
| MsG0280010564.01 | MsG0880044564.01 | 0.827069 | 1.917289e-54 | 1.831112e-51 |
| MsG0280011073.01 | MsG0880044564.01 | 0.851684 | 7.561913e-61 | 1.542680e-57 |
| MsG0380012868.01 | MsG0880044564.01 | 0.834167 | 3.504182e-56 | 4.124347e-53 |
| MsG0380014588.01 | MsG0880044564.01 | 0.801446 | 8.967927e-49 | 4.283670e-46 |
| MsG0680031469.01 | MsG0880044564.01 | 0.801746 | 7.783558e-49 | 3.746178e-46 |
| MsG0680032830.01 | MsG0880044564.01 | 0.811153 | 8.074239e-51 | 4.960472e-48 |
| MsG0680032850.01 | MsG0880044564.01 | 0.843080 | 1.745535e-58 | 2.700047e-55 |
| MsG0680033377.01 | MsG0880044564.01 | 0.801878 | 7.314264e-49 | 3.532336e-46 |
| MsG0680034064.01 | MsG0880044564.01 | 0.802170 | 6.369911e-49 | 3.099053e-46 |
| MsG0480018644.01 | MsG0880044564.01 | 0.803661 | 3.133796e-49 | 1.583812e-46 |
| MsG0480021168.01 | MsG0880044564.01 | 0.802163 | 6.391028e-49 | 3.108782e-46 |
| MsG0480022155.01 | MsG0880044564.01 | 0.814051 | 1.875579e-51 | 1.245344e-48 |
| MsG0480022162.01 | MsG0880044564.01 | 0.801672 | 8.061438e-49 | 3.872636e-46 |
| MsG0480022239.01 | MsG0880044564.01 | 0.864348 | 1.297580e-64 | 4.060980e-61 |
| MsG0480022240.01 | MsG0880044564.01 | 0.820637 | 6.172697e-53 | 4.910785e-50 |
| MsG0480022243.01 | MsG0880044564.01 | 0.808932 | 2.429362e-50 | 1.407090e-47 |
| MsG0480022245.01 | MsG0880044564.01 | 0.811340 | 7.353707e-51 | 4.539954e-48 |
| MsG0480022647.01 | MsG0880044564.01 | 0.803971 | 2.702422e-49 | 1.376780e-46 |
| MsG0480022872.01 | MsG0880044564.01 | -0.805611 | 1.227843e-49 | 6.524865e-47 |
| MsG0480023406.01 | MsG0880044564.01 | 0.805239 | 1.469481e-49 | 7.734595e-47 |
| MsG0580024285.01 | MsG0880044564.01 | 0.803086 | 4.122479e-49 | 2.052893e-46 |
| MsG0580024873.01 | MsG0880044564.01 | 0.812143 | 4.915980e-51 | 3.100996e-48 |
| MsG0380015438.01 | MsG0880044564.01 | 0.825991 | 3.465740e-54 | 3.209233e-51 |
| MsG0380015547.01 | MsG0880044564.01 | 0.809191 | 2.137173e-50 | 1.246609e-47 |
| MsG0380016057.01 | MsG0880044564.01 | 0.826017 | 3.415924e-54 | 3.165482e-51 |
| MsG0380016194.01 | MsG0880044564.01 | 0.843124 | 1.698756e-58 | 2.631424e-55 |
| MsG0380016284.01 | MsG0880044564.01 | 0.802823 | 4.673340e-49 | 2.311941e-46 |
| MsG0380017338.01 | MsG0880044564.01 | 0.805911 | 1.062226e-49 | 5.688510e-47 |
| MsG0580028024.01 | MsG0880044564.01 | 0.823690 | 1.209455e-53 | 1.048968e-50 |
| MsG0580029312.01 | MsG0880044564.01 | 0.800442 | 1.438676e-48 | 6.699575e-46 |
| MsG0580029325.01 | MsG0880044564.01 | 0.800463 | 1.424489e-48 | 6.636737e-46 |
| MsG0580029734.01 | MsG0880044564.01 | 0.811133 | 8.152707e-51 | 5.006250e-48 |
| MsG0580029912.01 | MsG0880044564.01 | 0.840927 | 6.472739e-58 | 9.360944e-55 |
| MsG0580029914.01 | MsG0880044564.01 | 0.806597 | 7.616079e-50 | 4.150503e-47 |
| MsG0680030464.01 | MsG0880044564.01 | 0.817935 | 2.547578e-52 | 1.880714e-49 |
| MsG0280008928.01 | MsG0880044564.01 | 0.831383 | 1.722180e-55 | 1.865873e-52 |
| MsG0280008929.01 | MsG0880044564.01 | 0.833235 | 5.990247e-56 | 6.856717e-53 |
| MsG0280009685.01 | MsG0880044564.01 | 0.818505 | 1.892764e-52 | 1.419430e-49 |
| MsG0280009756.01 | MsG0880044564.01 | 0.804892 | 1.736618e-49 | 9.060690e-47 |
| MsG0280010353.01 | MsG0880044564.01 | 0.800561 | 1.360305e-48 | 6.353667e-46 |
| MsG0780037363.01 | MsG0880044564.01 | 0.802668 | 5.030098e-49 | 2.478394e-46 |
| MsG0780037662.01 | MsG0880044564.01 | 0.840162 | 1.026336e-57 | 1.449647e-54 |
| MsG0780037701.01 | MsG0880044564.01 | 0.829590 | 4.727293e-55 | 4.859891e-52 |
| MsG0780039964.01 | MsG0880044564.01 | 0.817873 | 2.631122e-52 | 1.939106e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880044564.01 | MsG0680035693.01 | PPI |
| MsG0880044564.01 | MsG0680035693.01 | PPI |
| MsG0880044564.01 | MsG0880047062.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044564.01.T01 | MTR_8g464690 | 99.083 | 109 | 1 | 0 | 6 | 114 | 1 | 109 | 3.16e-77 | 223 |
| MsG0880044564.01.T01 | MTR_6g087770 | 51.087 | 92 | 45 | 0 | 6 | 97 | 1 | 92 | 4.60e-30 | 103 |
| MsG0880044564.01.T01 | MTR_2g101850 | 55.556 | 90 | 40 | 0 | 6 | 95 | 1 | 90 | 1.46e-29 | 102 |
| MsG0880044564.01.T01 | MTR_4g120150 | 48.837 | 86 | 42 | 1 | 10 | 95 | 46 | 129 | 4.18e-26 | 94.7 |
| MsG0880044564.01.T01 | MTR_4g114060 | 49.367 | 79 | 40 | 0 | 16 | 94 | 159 | 237 | 6.72e-22 | 87.0 |
| MsG0880044564.01.T01 | MTR_8g104900 | 45.882 | 85 | 44 | 1 | 17 | 99 | 45 | 129 | 2.09e-19 | 77.8 |
| MsG0880044564.01.T01 | MTR_1g031880 | 39.796 | 98 | 56 | 2 | 5 | 99 | 46 | 143 | 3.68e-19 | 77.4 |
| MsG0880044564.01.T01 | MTR_4g107350 | 45.652 | 92 | 48 | 2 | 4 | 94 | 210 | 300 | 2.11e-18 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044564.01.T01 | AT4G27360 | 71.845 | 103 | 29 | 0 | 6 | 108 | 1 | 103 | 1.79e-50 | 155 |
| MsG0880044564.01.T01 | AT3G16120 | 55.435 | 92 | 41 | 0 | 6 | 97 | 1 | 92 | 6.02e-33 | 110 |
| MsG0880044564.01.T01 | AT3G16120 | 55.435 | 92 | 41 | 0 | 6 | 97 | 1 | 92 | 6.02e-33 | 110 |
| MsG0880044564.01.T01 | AT1G52245 | 51.064 | 94 | 46 | 0 | 6 | 99 | 1 | 94 | 4.02e-30 | 103 |
| MsG0880044564.01.T01 | AT4G15930 | 49.412 | 85 | 41 | 1 | 10 | 94 | 39 | 121 | 4.34e-24 | 89.4 |
| MsG0880044564.01.T01 | AT5G20110 | 46.988 | 83 | 44 | 0 | 17 | 99 | 123 | 205 | 4.26e-21 | 84.0 |
| MsG0880044564.01.T01 | AT1G23220 | 39.773 | 88 | 52 | 1 | 13 | 99 | 38 | 125 | 4.68e-18 | 74.3 |
| MsG0880044564.01.T01 | AT4G15930 | 49.180 | 61 | 29 | 1 | 10 | 70 | 39 | 97 | 1.54e-13 | 61.6 |
Find 23 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTAGGCACTGATTT+TGG | 0.221609 | 8:-44582242 | MsG0880044564.01.T01:CDS |
| CAGGAATTTGATAGGGTTCA+TGG | 0.298316 | 8:-44582278 | MsG0880044564.01.T01:CDS |
| CAGTTACATCATAGAAATCT+AGG | 0.300776 | 8:+44582422 | None:intergenic |
| AATTATTGCAGGAATTTGAT+AGG | 0.302502 | 8:-44582286 | MsG0880044564.01.T01:intron |
| ATCATAGAAATCTAGGGCTT+TGG | 0.353606 | 8:+44582429 | None:intergenic |
| CTTCAATTCATCATGCTAGA+AGG | 0.354098 | 8:-44582517 | MsG0880044564.01.T01:CDS |
| TTGCTCTTTAAGGGATCTGC+TGG | 0.420352 | 8:-44582158 | MsG0880044564.01.T01:CDS |
| CCTGGTTGGCAATGCATTGT+AGG | 0.440795 | 8:-44582254 | MsG0880044564.01.T01:CDS |
| TTGCATAGTTTGAAGCATAT+CGG | 0.449905 | 8:+44582474 | None:intergenic |
| CTAGAAGGCAAGGCAGTGAT+TGG | 0.481296 | 8:-44582502 | MsG0880044564.01.T01:CDS |
| CCTACAATGCATTGCCAACC+AGG | 0.504627 | 8:+44582254 | None:intergenic |
| AAATCAGTTCTCAGCAATAG+AGG | 0.512873 | 8:-44582120 | MsG0880044564.01.T01:CDS |
| TATAGCTCGATTTATAAAGA+AGG | 0.514762 | 8:-44582389 | MsG0880044564.01.T01:intron |
| TTTGATAGGGTTCATGGACC+TGG | 0.522418 | 8:-44582272 | MsG0880044564.01.T01:CDS |
| ATTATTGCAGGAATTTGATA+GGG | 0.524356 | 8:-44582285 | MsG0880044564.01.T01:intron |
| TATAAATCGAGCTATATCAA+TGG | 0.561045 | 8:+44582396 | None:intergenic |
| ATCTGCTGGTCCACAAGCAG+AGG | 0.571115 | 8:-44582144 | MsG0880044564.01.T01:CDS |
| ATTCATCATGCTAGAAGGCA+AGG | 0.581622 | 8:-44582512 | MsG0880044564.01.T01:CDS |
| TCCTTTGTGACACATTGTAG+TGG | 0.583602 | 8:-44582218 | MsG0880044564.01.T01:CDS |
| AGTTACATCATAGAAATCTA+GGG | 0.585718 | 8:+44582423 | None:intergenic |
| ATAGGGTTCATGGACCTGGT+TGG | 0.594187 | 8:-44582268 | MsG0880044564.01.T01:CDS |
| GCCACTACAATGTGTCACAA+AGG | 0.659746 | 8:+44582217 | None:intergenic |
| AGATTTCTATGATGTAACTG+AGG | 0.680263 | 8:-44582419 | MsG0880044564.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATGTAATTTTTTTTG+TGG | - | Chr8:44582296-44582315 | MsG0880044564.01.T01:intron | 10.0% |
| !! | GGTAATAAATTAAAACTAAG+AGG | - | Chr8:44582258-44582277 | MsG0880044564.01.T01:CDS | 20.0% |
| ! | AAACTAAGAGGATTGTTTAT+TGG | - | Chr8:44582270-44582289 | MsG0880044564.01.T01:CDS | 25.0% |
| ! | AATTATTGCAGGAATTTGAT+AGG | - | Chr8:44582340-44582359 | MsG0880044564.01.T01:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | + | Chr8:44582206-44582225 | None:intergenic | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | - | Chr8:44582341-44582360 | MsG0880044564.01.T01:intron | 25.0% |
| ! | GATATGTTGTTAATTATTGC+AGG | - | Chr8:44582329-44582348 | MsG0880044564.01.T01:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | + | Chr8:44582233-44582252 | None:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | - | Chr8:44582237-44582256 | MsG0880044564.01.T01:CDS | 25.0% |
| !!! | CTATTTTTGTATAGGTAGTA+TGG | - | Chr8:44582440-44582459 | MsG0880044564.01.T01:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | - | Chr8:44582432-44582451 | MsG0880044564.01.T01:CDS | 25.0% |
| AGATTTCTATGATGTAACTG+AGG | - | Chr8:44582207-44582226 | MsG0880044564.01.T01:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | + | Chr8:44582207-44582226 | None:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | - | Chr8:44582459-44582478 | MsG0880044564.01.T01:CDS | 30.0% |
| ! | TATGGCAATTTTGCTCTTTA+AGG | - | Chr8:44582458-44582477 | MsG0880044564.01.T01:CDS | 30.0% |
| ! | TTGCATAGTTTGAAGCATAT+CGG | + | Chr8:44582155-44582174 | None:intergenic | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | - | Chr8:44582506-44582525 | MsG0880044564.01.T01:CDS | 35.0% | |
| CTTCAATTCATCATGCTAGA+AGG | - | Chr8:44582109-44582128 | MsG0880044564.01.T01:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | + | Chr8:44582200-44582219 | None:intergenic | 35.0% |
| ATTCATCATGCTAGAAGGCA+AGG | - | Chr8:44582114-44582133 | MsG0880044564.01.T01:CDS | 40.0% | |
| CAGGAATTTGATAGGGTTCA+TGG | - | Chr8:44582348-44582367 | MsG0880044564.01.T01:intron | 40.0% | |
| TCCTTTGTGACACATTGTAG+TGG | - | Chr8:44582408-44582427 | MsG0880044564.01.T01:CDS | 40.0% | |
| !! | AACTGATTTTCCTCTGCTTG+TGG | + | Chr8:44582495-44582514 | None:intergenic | 40.0% |
| !!! | TGCATTGTAGGCACTGATTT+TGG | - | Chr8:44582384-44582403 | MsG0880044564.01.T01:intron | 40.0% |
| GCCACTACAATGTGTCACAA+AGG | + | Chr8:44582412-44582431 | None:intergenic | 45.0% | |
| TTTGATAGGGTTCATGGACC+TGG | - | Chr8:44582354-44582373 | MsG0880044564.01.T01:intron | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | - | Chr8:44582468-44582487 | MsG0880044564.01.T01:CDS | 45.0% |
| ATAGGGTTCATGGACCTGGT+TGG | - | Chr8:44582358-44582377 | MsG0880044564.01.T01:intron | 50.0% | |
| CCTACAATGCATTGCCAACC+AGG | + | Chr8:44582375-44582394 | None:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | - | Chr8:44582372-44582391 | MsG0880044564.01.T01:intron | 50.0% | |
| CTAGAAGGCAAGGCAGTGAT+TGG | - | Chr8:44582124-44582143 | MsG0880044564.01.T01:CDS | 50.0% | |
| ATCTGCTGGTCCACAAGCAG+AGG | - | Chr8:44582482-44582501 | MsG0880044564.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 44582106 | 44582542 | 44582106 | ID=MsG0880044564.01;Name=MsG0880044564.01 |
| Chr8 | mRNA | 44582106 | 44582542 | 44582106 | ID=MsG0880044564.01.T01;Parent=MsG0880044564.01;Name=MsG0880044564.01.T01;_AED=0.38;_eAED=0.39;_QI=0|0|0|1|1|1|2|0|114 |
| Chr8 | exon | 44582390 | 44582542 | 44582390 | ID=MsG0880044564.01.T01:exon:18036;Parent=MsG0880044564.01.T01 |
| Chr8 | exon | 44582106 | 44582297 | 44582106 | ID=MsG0880044564.01.T01:exon:18035;Parent=MsG0880044564.01.T01 |
| Chr8 | CDS | 44582390 | 44582542 | 44582390 | ID=MsG0880044564.01.T01:cds;Parent=MsG0880044564.01.T01 |
| Chr8 | CDS | 44582106 | 44582297 | 44582106 | ID=MsG0880044564.01.T01:cds;Parent=MsG0880044564.01.T01 |
| Gene Sequence |
| Protein sequence |
