Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044523.01.T01 | XP_013445572.1 | 98.165 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 1.22E-72 | 221 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044523.01.T01 | O96860 | 55.556 | 90 | 38 | 1 | 1 | 90 | 1 | 88 | 5.91E-29 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044523.01.T01 | A0A072TRW8 | 98.165 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 5.80e-73 | 221 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880044523.01 | MsG0880044524.01 | 0.934396 | 4.311139e-96 | 3.250699e-91 |
| MsG0880044523.01 | MsG0880044564.01 | 0.859776 | 3.272935e-63 | 8.745092e-60 |
| MsG0280011073.01 | MsG0880044523.01 | 0.805776 | 1.133910e-49 | 6.051541e-47 |
| MsG0380012868.01 | MsG0880044523.01 | 0.808240 | 3.412884e-50 | 1.941382e-47 |
| MsG0680032850.01 | MsG0880044523.01 | 0.811186 | 7.940235e-51 | 4.882496e-48 |
| MsG0480022239.01 | MsG0880044523.01 | 0.826731 | 2.309283e-54 | 2.183824e-51 |
| MsG0480022245.01 | MsG0880044523.01 | 0.809251 | 2.075574e-50 | 1.212597e-47 |
| MsG0480022248.01 | MsG0880044523.01 | 0.813916 | 2.009187e-51 | 1.329160e-48 |
| MsG0480022673.01 | MsG0880044523.01 | -0.828706 | 7.743650e-55 | 7.756973e-52 |
| MsG0580029325.01 | MsG0880044523.01 | 0.817166 | 3.797086e-52 | 2.744704e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880047062.01 | MsG0880044523.01 | PPI |
| MsG0880044523.01 | MsG0680035693.01 | PPI |
| MsG0680035693.01 | MsG0880044523.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044523.01.T01 | MTR_8g464690 | 98.165 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 1.47e-76 | 221 |
| MsG0880044523.01.T01 | MTR_6g087770 | 51.087 | 92 | 45 | 0 | 1 | 92 | 1 | 92 | 4.21e-30 | 103 |
| MsG0880044523.01.T01 | MTR_2g101850 | 55.556 | 90 | 40 | 0 | 1 | 90 | 1 | 90 | 2.05e-29 | 101 |
| MsG0880044523.01.T01 | MTR_4g120150 | 48.837 | 86 | 42 | 1 | 5 | 90 | 46 | 129 | 8.21e-26 | 94.0 |
| MsG0880044523.01.T01 | MTR_4g114060 | 49.367 | 79 | 40 | 0 | 11 | 89 | 159 | 237 | 1.20e-21 | 85.9 |
| MsG0880044523.01.T01 | MTR_8g104900 | 45.882 | 85 | 44 | 1 | 12 | 94 | 45 | 129 | 4.23e-19 | 76.6 |
| MsG0880044523.01.T01 | MTR_1g031880 | 43.529 | 85 | 46 | 1 | 12 | 94 | 59 | 143 | 7.50e-19 | 76.6 |
| MsG0880044523.01.T01 | MTR_4g107350 | 50.633 | 79 | 38 | 1 | 12 | 89 | 222 | 300 | 3.48e-18 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044523.01.T01 | AT4G27360 | 70.874 | 103 | 30 | 0 | 1 | 103 | 1 | 103 | 1.33e-49 | 153 |
| MsG0880044523.01.T01 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 8.80e-33 | 110 |
| MsG0880044523.01.T01 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 8.80e-33 | 110 |
| MsG0880044523.01.T01 | AT1G52245 | 51.064 | 94 | 46 | 0 | 1 | 94 | 1 | 94 | 4.11e-30 | 103 |
| MsG0880044523.01.T01 | AT4G15930 | 49.412 | 85 | 41 | 1 | 5 | 89 | 39 | 121 | 7.44e-24 | 88.6 |
| MsG0880044523.01.T01 | AT5G20110 | 46.988 | 83 | 44 | 0 | 12 | 94 | 123 | 205 | 5.24e-21 | 83.6 |
| MsG0880044523.01.T01 | AT1G23220 | 39.773 | 88 | 52 | 1 | 8 | 94 | 38 | 125 | 5.70e-18 | 73.9 |
| MsG0880044523.01.T01 | AT4G15930 | 49.180 | 61 | 29 | 1 | 5 | 65 | 39 | 97 | 1.87e-13 | 61.2 |
Find 22 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTAGGCACTGATTT+TGG | 0.221609 | 8:+43805982 | MsG0880044523.01.T01:CDS |
| CAGTTACATCATAGAAATCT+AGG | 0.300776 | 8:-43805801 | None:intergenic |
| AATTATTGCAGGAATTTGAT+AGG | 0.302502 | 8:+43805938 | MsG0880044523.01.T01:intron |
| ATCATAGAAATCTAGGGCTT+TGG | 0.353606 | 8:-43805794 | None:intergenic |
| CTTCAATTCATCATGCTAGA+AGG | 0.354098 | 8:+43805706 | None:intergenic |
| TTGCTCTTTAAGGGATCTGC+TGG | 0.420352 | 8:+43806066 | MsG0880044523.01.T01:CDS |
| CCTGGTTGGCAATGCATTGT+AGG | 0.440795 | 8:+43805970 | MsG0880044523.01.T01:CDS |
| CTAGAAGGCAAGGCAGTGAT+TGG | 0.481296 | 8:+43805721 | MsG0880044523.01.T01:CDS |
| CCTACAATGCATTGCCAACC+AGG | 0.504627 | 8:-43805970 | None:intergenic |
| AAATCAGTTCTCAGCAATAG+AGG | 0.512873 | 8:+43806104 | MsG0880044523.01.T01:CDS |
| TATAGCTCGATTTATAAAGA+AGG | 0.514762 | 8:+43805834 | MsG0880044523.01.T01:CDS |
| ATTATTGCAGGAATTTGATA+GGG | 0.521916 | 8:+43805939 | MsG0880044523.01.T01:intron |
| CAGGAATTTGATAGGGTGCA+TGG | 0.526835 | 8:+43805946 | MsG0880044523.01.T01:intron |
| TTTGATAGGGTGCATGGACC+TGG | 0.541477 | 8:+43805952 | MsG0880044523.01.T01:CDS |
| TATAAATCGAGCTATATCAA+TGG | 0.561045 | 8:-43805827 | None:intergenic |
| ATCTGCTGGTCCACAAGCAG+AGG | 0.571115 | 8:+43806080 | MsG0880044523.01.T01:CDS |
| ATTCATCATGCTAGAAGGCA+AGG | 0.581622 | 8:+43805711 | None:intergenic |
| TCCTTTGTGACACATTGTAG+TGG | 0.583602 | 8:+43806006 | MsG0880044523.01.T01:CDS |
| AGTTACATCATAGAAATCTA+GGG | 0.585718 | 8:-43805800 | None:intergenic |
| ATAGGGTGCATGGACCTGGT+TGG | 0.652898 | 8:+43805956 | MsG0880044523.01.T01:CDS |
| GCCACTACAATGTGTCACAA+AGG | 0.659746 | 8:-43806007 | None:intergenic |
| AGATTTCTATGATGTAACTG+AGG | 0.680263 | 8:+43805804 | MsG0880044523.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTATTGCAGGAATTTGAT+AGG | + | Chr8:43805938-43805957 | MsG0880044523.01.T01:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | - | Chr8:43805803-43805822 | None:intergenic | 25.0% |
| ! | ATGTGTTAGTTAATTATTGC+AGG | + | Chr8:43805927-43805946 | MsG0880044523.01.T01:intron | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | + | Chr8:43805939-43805958 | MsG0880044523.01.T01:intron | 25.0% |
| ! | GGTAATAAAATAAAACCAAG+AGG | + | Chr8:43805855-43805874 | MsG0880044523.01.T01:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | - | Chr8:43805830-43805849 | None:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | + | Chr8:43805834-43805853 | MsG0880044523.01.T01:CDS | 25.0% |
| !!! | CTATTTTTGTATAGGTAGTA+TGG | + | Chr8:43806038-43806057 | MsG0880044523.01.T01:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | + | Chr8:43806030-43806049 | MsG0880044523.01.T01:CDS | 25.0% |
| AAACCAAGAGGATTGTTTAT+TGG | + | Chr8:43805867-43805886 | MsG0880044523.01.T01:intron | 30.0% | |
| AGATTTCTATGATGTAACTG+AGG | + | Chr8:43805804-43805823 | MsG0880044523.01.T01:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | - | Chr8:43805804-43805823 | None:intergenic | 30.0% | |
| TAACCAATAAACAATCCTCT+TGG | - | Chr8:43805873-43805892 | None:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | + | Chr8:43806057-43806076 | MsG0880044523.01.T01:CDS | 30.0% |
| ! | TATGGCAATTTTGCTCTTTA+AGG | + | Chr8:43806056-43806075 | MsG0880044523.01.T01:CDS | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | + | Chr8:43806104-43806123 | MsG0880044523.01.T01:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | - | Chr8:43805797-43805816 | None:intergenic | 35.0% |
| TCCTTTGTGACACATTGTAG+TGG | + | Chr8:43806006-43806025 | MsG0880044523.01.T01:CDS | 40.0% | |
| !! | AACTGATTTTCCTCTGCTTG+TGG | - | Chr8:43806093-43806112 | None:intergenic | 40.0% |
| !!! | TGCATTGTAGGCACTGATTT+TGG | + | Chr8:43805982-43806001 | MsG0880044523.01.T01:CDS | 40.0% |
| CAGGAATTTGATAGGGTGCA+TGG | + | Chr8:43805946-43805965 | MsG0880044523.01.T01:intron | 45.0% | |
| GCCACTACAATGTGTCACAA+AGG | - | Chr8:43806010-43806029 | None:intergenic | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | + | Chr8:43806066-43806085 | MsG0880044523.01.T01:CDS | 45.0% |
| !!! | TTTTAATGTAATTTTTTTTA+TGG | + | Chr8:43805893-43805912 | MsG0880044523.01.T01:intron | 5.0% |
| CCTACAATGCATTGCCAACC+AGG | - | Chr8:43805973-43805992 | None:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | + | Chr8:43805970-43805989 | MsG0880044523.01.T01:CDS | 50.0% | |
| CTAGAAGGCAAGGCAGTGAT+TGG | + | Chr8:43805721-43805740 | MsG0880044523.01.T01:CDS | 50.0% | |
| TTTGATAGGGTGCATGGACC+TGG | + | Chr8:43805952-43805971 | MsG0880044523.01.T01:CDS | 50.0% | |
| ATAGGGTGCATGGACCTGGT+TGG | + | Chr8:43805956-43805975 | MsG0880044523.01.T01:CDS | 55.0% | |
| ATCTGCTGGTCCACAAGCAG+AGG | + | Chr8:43806080-43806099 | MsG0880044523.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 43805718 | 43806140 | 43805718 | ID=MsG0880044523.01;Name=MsG0880044523.01 |
| Chr8 | mRNA | 43805718 | 43806140 | 43805718 | ID=MsG0880044523.01.T01;Parent=MsG0880044523.01;Name=MsG0880044523.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|109 |
| Chr8 | exon | 43805718 | 43805855 | 43805718 | ID=MsG0880044523.01.T01:exon:8295;Parent=MsG0880044523.01.T01 |
| Chr8 | exon | 43805949 | 43806140 | 43805949 | ID=MsG0880044523.01.T01:exon:8294;Parent=MsG0880044523.01.T01 |
| Chr8 | CDS | 43805718 | 43805855 | 43805718 | ID=MsG0880044523.01.T01:cds;Parent=MsG0880044523.01.T01 |
| Chr8 | CDS | 43805949 | 43806140 | 43805949 | ID=MsG0880044523.01.T01:cds;Parent=MsG0880044523.01.T01 |
| Gene Sequence |
| Protein sequence |
