Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047314.01.T01 | XP_003609952.1 | 100 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 8.62E-85 | 253 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047314.01.T01 | Q29PU2 | 51.908 | 131 | 50 | 5 | 1 | 122 | 1 | 127 | 3.75E-34 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780040978.01 | MsG0880047314.01 | 0.829177 | 5.956096e-55 | 6.049717e-52 |
| MsG0780040990.01 | MsG0880047314.01 | 0.807207 | 5.657023e-50 | 3.132060e-47 |
| MsG0580026343.01 | MsG0880047314.01 | 0.846889 | 1.635286e-59 | 2.855671e-56 |
| MsG0580027854.01 | MsG0880047314.01 | 0.818913 | 1.528972e-52 | 1.159713e-49 |
| MsG0580030141.01 | MsG0880047314.01 | 0.809021 | 2.324908e-50 | 1.349831e-47 |
| MsG0780038647.01 | MsG0880047314.01 | 0.837375 | 5.394684e-57 | 6.993354e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047314.01.T01 | MTR_4g124700 | 100.000 | 122 | 0 | 0 | 1 | 122 | 1 | 122 | 1.04e-88 | 253 |
| MsG0880047314.01.T01 | MTR_2g044020 | 61.240 | 129 | 40 | 4 | 1 | 122 | 1 | 126 | 5.54e-45 | 142 |
| MsG0880047314.01.T01 | MTR_8g022440 | 53.226 | 124 | 50 | 3 | 1 | 122 | 1 | 118 | 1.16e-25 | 93.6 |
| MsG0880047314.01.T01 | MTR_8g022380 | 52.419 | 124 | 49 | 3 | 1 | 122 | 1 | 116 | 1.06e-24 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047314.01.T01 | AT5G20820 | 51.908 | 131 | 50 | 5 | 1 | 122 | 1 | 127 | 3.83e-35 | 117 |
| MsG0880047314.01.T01 | AT1G72430 | 50.413 | 121 | 57 | 2 | 1 | 121 | 1 | 118 | 6.94e-35 | 117 |
| MsG0880047314.01.T01 | AT1G17345 | 47.328 | 131 | 58 | 4 | 1 | 121 | 1 | 130 | 2.23e-30 | 105 |
| MsG0880047314.01.T01 | AT3G12955 | 32.727 | 110 | 68 | 2 | 17 | 120 | 27 | 136 | 1.26e-13 | 63.2 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGTTCATCAAGAGATTC+AGG | 0.295459 | 8:-84539434 | None:intergenic |
| GGGACCTTTCAACGAGTTCA+CGG | 0.472584 | 8:-84539313 | None:intergenic |
| TCAACGAGTTCACGGAAGAC+AGG | 0.474752 | 8:-84539305 | None:intergenic |
| TCATCAAGAGATTCAGGTTG+AGG | 0.474875 | 8:-84539428 | None:intergenic |
| TCCATAACAAATGTTCGAAA+AGG | 0.493584 | 8:-84539388 | None:intergenic |
| TACAAACCAAACATGGCAAA+AGG | 0.496903 | 8:+84539095 | None:intergenic |
| AAATGGAACTCATTTGGAAA+CGG | 0.515566 | 8:+84539152 | MsG0880047314.01.T01:CDS |
| CGGCGACGGCGCTGATGCTG+TGG | 0.518156 | 8:-84539187 | None:intergenic |
| AGAGGAATCTTCGTCGGCGA+CGG | 0.535393 | 8:-84539201 | None:intergenic |
| TTCGAAAAGGACAACCTCAC+AGG | 0.546579 | 8:-84539375 | None:intergenic |
| CAACGAGTTCACGGAAGACA+GGG | 0.547669 | 8:-84539304 | None:intergenic |
| TCTTCCGTGAACTCGTTGAA+AGG | 0.553015 | 8:+84539309 | MsG0880047314.01.T01:CDS |
| CGACGGCGCTGATGCTGTGG+CGG | 0.569425 | 8:-84539184 | None:intergenic |
| CAACAACGTCGGAGGTGACA+CGG | 0.574056 | 8:-84539277 | None:intergenic |
| TACGAAGACGGTGTGGAGGT+CGG | 0.582641 | 8:-84539234 | None:intergenic |
| GCGAGACTTGCCTACGAAGA+CGG | 0.593143 | 8:-84539246 | None:intergenic |
| GAGGTCGGATCTTGAAGAAG+AGG | 0.595903 | 8:-84539219 | None:intergenic |
| TGTCAATGTTGTTGCCTGTG+AGG | 0.609545 | 8:+84539361 | MsG0880047314.01.T01:CDS |
| AAACCAAACATGGCAAAAGG+TGG | 0.619508 | 8:+84539098 | None:intergenic |
| CGGAGGTGACACGGTAGAGA+CGG | 0.621920 | 8:-84539268 | None:intergenic |
| TGAAGAAGAGGAATCTTCGT+CGG | 0.636980 | 8:-84539207 | None:intergenic |
| TGCCTACGAAGACGGTGTGG+AGG | 0.660845 | 8:-84539238 | None:intergenic |
| GACAGGGTTGTCAACAACGT+CGG | 0.661820 | 8:-84539288 | None:intergenic |
| ACTTGCCTACGAAGACGGTG+TGG | 0.680608 | 8:-84539241 | None:intergenic |
| GACCTCCACACCGTCTTCGT+AGG | 0.682243 | 8:+84539236 | MsG0880047314.01.T01:CDS |
| AGGGTTGTCAACAACGTCGG+AGG | 0.683208 | 8:-84539285 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAATCAGCACTCAAAAAA+TGG | + | Chr8:84539135-84539154 | MsG0880047314.01.T01:CDS | 25.0% |
| AAATGGAACTCATTTGGAAA+CGG | + | Chr8:84539152-84539171 | MsG0880047314.01.T01:CDS | 30.0% | |
| CTCAAAAAATGGAACTCATT+TGG | + | Chr8:84539146-84539165 | MsG0880047314.01.T01:CDS | 30.0% | |
| TCCATAACAAATGTTCGAAA+AGG | - | Chr8:84539391-84539410 | None:intergenic | 30.0% | |
| !! | TCCTTTTCGAACATTTGTTA+TGG | + | Chr8:84539387-84539406 | MsG0880047314.01.T01:CDS | 30.0% |
| ACAAGTTCATCAAGAGATTC+AGG | - | Chr8:84539437-84539456 | None:intergenic | 35.0% | |
| !!! | CGTTTTGTTGTTCTGTTTCA+CGG | - | Chr8:84539337-84539356 | None:intergenic | 35.0% |
| !!! | GTTTTGTTGTTCTGTTTCAC+GGG | - | Chr8:84539336-84539355 | None:intergenic | 35.0% |
| TGAAGAAGAGGAATCTTCGT+CGG | - | Chr8:84539210-84539229 | None:intergenic | 40.0% | |
| !! | TCATCAAGAGATTCAGGTTG+AGG | - | Chr8:84539431-84539450 | None:intergenic | 40.0% |
| TCTTCCGTGAACTCGTTGAA+AGG | + | Chr8:84539309-84539328 | MsG0880047314.01.T01:CDS | 45.0% | |
| TGTCAATGTTGTTGCCTGTG+AGG | + | Chr8:84539361-84539380 | MsG0880047314.01.T01:CDS | 45.0% | |
| TTCGAAAAGGACAACCTCAC+AGG | - | Chr8:84539378-84539397 | None:intergenic | 45.0% | |
| CAACGAGTTCACGGAAGACA+GGG | - | Chr8:84539307-84539326 | None:intergenic | 50.0% | |
| GACAGGGTTGTCAACAACGT+CGG | - | Chr8:84539291-84539310 | None:intergenic | 50.0% | |
| GAGGTCGGATCTTGAAGAAG+AGG | - | Chr8:84539222-84539241 | None:intergenic | 50.0% | |
| GGGACCTTTCAACGAGTTCA+CGG | - | Chr8:84539316-84539335 | None:intergenic | 50.0% | |
| TCAACGAGTTCACGGAAGAC+AGG | - | Chr8:84539308-84539327 | None:intergenic | 50.0% | |
| ACTTGCCTACGAAGACGGTG+TGG | - | Chr8:84539244-84539263 | None:intergenic | 55.0% | |
| AGAGGAATCTTCGTCGGCGA+CGG | - | Chr8:84539204-84539223 | None:intergenic | 55.0% | |
| AGGGTTGTCAACAACGTCGG+AGG | - | Chr8:84539288-84539307 | None:intergenic | 55.0% | |
| CAACAACGTCGGAGGTGACA+CGG | - | Chr8:84539280-84539299 | None:intergenic | 55.0% | |
| GCGAGACTTGCCTACGAAGA+CGG | - | Chr8:84539249-84539268 | None:intergenic | 55.0% | |
| TACGAAGACGGTGTGGAGGT+CGG | - | Chr8:84539237-84539256 | None:intergenic | 55.0% | |
| CGGAGGTGACACGGTAGAGA+CGG | - | Chr8:84539271-84539290 | None:intergenic | 60.0% | |
| GACCTCCACACCGTCTTCGT+AGG | + | Chr8:84539236-84539255 | MsG0880047314.01.T01:CDS | 60.0% | |
| TGCCTACGAAGACGGTGTGG+AGG | - | Chr8:84539241-84539260 | None:intergenic | 60.0% | |
| !! | CGACGGCGCTGATGCTGTGG+CGG | - | Chr8:84539187-84539206 | None:intergenic | 70.0% |
| !! | CGGCGACGGCGCTGATGCTG+TGG | - | Chr8:84539190-84539209 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 84539107 | 84539475 | 84539107 | ID=MsG0880047314.01;Name=MsG0880047314.01 |
| Chr8 | mRNA | 84539107 | 84539475 | 84539107 | ID=MsG0880047314.01.T01;Parent=MsG0880047314.01;Name=MsG0880047314.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|122 |
| Chr8 | exon | 84539107 | 84539475 | 84539107 | ID=MsG0880047314.01.T01:exon:12486;Parent=MsG0880047314.01.T01 |
| Chr8 | CDS | 84539107 | 84539475 | 84539107 | ID=MsG0880047314.01.T01:cds;Parent=MsG0880047314.01.T01 |
| Gene Sequence |
| Protein sequence |
