AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030141.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030141.01.T01 MTR_5g098060 98.276 58 1 0 42 99 44 101 9.02e-33 115
MsG0580030141.01.T01 MTR_7g101080 45.312 64 34 1 36 98 29 92 8.59e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030141.01.T01 AT2G04780 80.000 55 11 0 45 99 45 99 9.27e-25 94.4
MsG0580030141.01.T01 AT2G04780 80.000 55 11 0 45 99 45 99 9.27e-25 94.4

Find 70 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTGTCAGCTATGGAATT+TGG 0.170303 5:+107232167 MsG0580030141.01.T01:three_prime_UTR
CAGTGTTGTTTGCTTGGTTT+TGG 0.201552 5:-107231679 None:intergenic
TTTGCTTCCTGAGGCAATTT+TGG 0.216260 5:+107232011 MsG0580030141.01.T01:three_prime_UTR
CCTTCAATCCACAAAAGTTT+TGG 0.254005 5:+107231649 MsG0580030141.01.T01:CDS
TGAATTTCACCCGATAATTC+TGG 0.267069 5:+107231895 MsG0580030141.01.T01:three_prime_UTR
CTTCTTCAGTGTTGTTTGCT+TGG 0.268289 5:-107231685 None:intergenic
AGAGGGTGGACTTACGGTTT+TGG 0.270515 5:-107231520 None:intergenic
GATTGTCAGCTATGGAATTT+GGG 0.295753 5:+107232168 MsG0580030141.01.T01:three_prime_UTR
GGAACTGTCAAAATTAATTC+AGG 0.296860 5:+107231916 MsG0580030141.01.T01:three_prime_UTR
GATGCGAAGGAACTGTCTTT+TGG 0.299960 5:-107231725 None:intergenic
CTCCCAGCACTTTGGTCAGC+TGG 0.303486 5:-107232097 None:intergenic
AACCTTGTGTTGGCAACATT+TGG 0.304302 5:+107232193 MsG0580030141.01.T01:three_prime_UTR
CACTGATATTCCACCTGTTC+CGG 0.305792 5:+107232034 MsG0580030141.01.T01:three_prime_UTR
ATCAGCTGCAGGCGCAATTC+TGG 0.316493 5:-107232071 None:intergenic
GTTGGCAACATTTGGTTTGT+TGG 0.348928 5:+107232201 MsG0580030141.01.T01:three_prime_UTR
TGGGCCGGAGTTGGACTCTC+GGG 0.349599 5:-107231546 None:intergenic
TCTGGTGTAGGTGCTGGTGC+TGG 0.359466 5:-107231566 None:intergenic
GTGGGTGCAGGAGCCGGAAC+AGG 0.378032 5:-107232047 None:intergenic
GGGCCGGAGTTGGACTCTCG+GGG 0.380390 5:-107231545 None:intergenic
TGCTGGTGCTGGGCCGGAGT+TGG 0.381215 5:-107231555 None:intergenic
ACTGAATTACTCACTGTTGC+TGG 0.384658 5:+107231602 MsG0580030141.01.T01:CDS
TTTGACAGTTCCAGAATTAT+CGG 0.386514 5:-107231905 None:intergenic
TCAGTGAGGTTAACAAAATC+TGG 0.394471 5:-107231584 None:intergenic
CGCAATTCTGGTGTGGGTGC+AGG 0.403708 5:-107232059 None:intergenic
CTGGGCCGGAGTTGGACTCT+CGG 0.408406 5:-107231547 None:intergenic
TGCAATTTATCAAGTTGATA+AGG 0.410347 5:+107231987 MsG0580030141.01.T01:three_prime_UTR
TCAACTTGATAAATTGCAAC+AGG 0.410462 5:-107231982 None:intergenic
TTGTAAGAAGACGAACTATC+TGG 0.411721 5:-107232145 None:intergenic
ATGTTGGAGTTGAGGCAGTT+TGG 0.434404 5:-107231853 None:intergenic
GGCATTAAAATCTTCAAGAA+AGG 0.436489 5:-107232459 None:intergenic
TATTGAAGAAAAGTGTGAAA+TGG 0.451994 5:-107231626 None:intergenic
GAGTTGAGGCAGTTTGGCTA+AGG 0.468259 5:-107231847 None:intergenic
CTGGTGTAGGTGCTGGTGCT+GGG 0.468334 5:-107231565 None:intergenic
TCTGGTGTGGGTGCAGGAGC+CGG 0.470278 5:-107232053 None:intergenic
TGTAGGTGCTGGTGCTGGGC+CGG 0.471397 5:-107231561 None:intergenic
TCTCCAGCTGACCAAAGTGC+TGG 0.473142 5:+107232094 MsG0580030141.01.T01:three_prime_UTR
TTGACAGTTCCAGAATTATC+GGG 0.480298 5:-107231904 None:intergenic
AAATGTGCAAAGCATGGAAA+AGG 0.484715 5:-107231800 None:intergenic
AGCTTTGAGTTGGATAAAGA+AGG 0.485300 5:-107231757 None:intergenic
TAATCTCATCAGCTTTGAGT+TGG 0.485850 5:-107231767 None:intergenic
GGCCGGAGTTGGACTCTCGG+GGG 0.486601 5:-107231544 None:intergenic
CAAGCAAACAACACTGAAGA+AGG 0.495908 5:+107231686 MsG0580030141.01.T01:CDS
GCCGGAGTTGGACTCTCGGG+GGG 0.498935 5:-107231543 None:intergenic
TCTTACAAGATTGTCAGCTA+TGG 0.505345 5:+107232160 MsG0580030141.01.T01:three_prime_UTR
ACGATTTGCTCCCAGCACTT+TGG 0.511706 5:-107232105 None:intergenic
GGCAACATTTGGTTTGTTGG+TGG 0.517913 5:+107232204 MsG0580030141.01.T01:three_prime_UTR
GCAACAGAGTGCAGCTCACA+AGG 0.522471 5:-107231476 None:intergenic
TGCAGGCGCAATTCTGGTGT+GGG 0.526669 5:-107232065 None:intergenic
ATCAGTGCCAAAATTGCCTC+AGG 0.528648 5:-107232018 None:intergenic
TCAGCTGGAGAATCAGCTGC+AGG 0.533442 5:-107232082 None:intergenic
GGTGCAGGAGCCGGAACAGG+TGG 0.536302 5:-107232044 None:intergenic
CTGCAGGCGCAATTCTGGTG+TGG 0.538512 5:-107232066 None:intergenic
CTCCAGCTGACCAAAGTGCT+GGG 0.544197 5:+107232095 MsG0580030141.01.T01:three_prime_UTR
TTCAGGATGGTCAATCACTA+AGG 0.555642 5:+107231933 MsG0580030141.01.T01:three_prime_UTR
GTAATCACCACGGCAAATGT+TGG 0.555987 5:-107231869 None:intergenic
AGGTTAACAAAATCTGGTGT+AGG 0.563929 5:-107231578 None:intergenic
GAATAGAAATGTGCAAAGCA+TGG 0.566393 5:-107231806 None:intergenic
ACAAAATCTGGTGTAGGTGC+TGG 0.568282 5:-107231572 None:intergenic
CACGGCAAATGTTGGAGTTG+AGG 0.571756 5:-107231861 None:intergenic
GAACTATCTGGTGAAGATGA+AGG 0.574002 5:-107232133 None:intergenic
AACCAAATGTTGCCAACACA+AGG 0.581175 5:-107232195 None:intergenic
AAGGTTCTTCAATGATGCGA+AGG 0.582579 5:-107231738 None:intergenic
CTGTCAAAATTAATTCAGGA+TGG 0.599822 5:+107231920 MsG0580030141.01.T01:three_prime_UTR
TCTGTTGCTTCTGAGCTCAT+CGG 0.605895 5:+107231491 MsG0580030141.01.T01:CDS
CTCAACTCCAACATTTGCCG+TGG 0.611075 5:+107231862 MsG0580030141.01.T01:three_prime_UTR
AATTTGGGGCAACCTTGTGT+TGG 0.628436 5:+107232183 MsG0580030141.01.T01:three_prime_UTR
ATTGTCAGCTATGGAATTTG+GGG 0.656452 5:+107232169 MsG0580030141.01.T01:three_prime_UTR
GTGGCACTCTTGTGATGATG+AGG 0.658950 5:+107232223 MsG0580030141.01.T01:three_prime_UTR
AAATTCAAGTGTAATCACCA+CGG 0.719863 5:-107231879 None:intergenic
CAACAGTGAGTAATTCAGTG+AGG 0.749189 5:-107231598 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATTATGTCACAAAAAAAT+TGG - Chr5:107232327-107232346 None:intergenic 20.0%
!! TATTGAAGAAAAGTGTGAAA+TGG - Chr5:107231629-107231648 None:intergenic 25.0%
!!! TGCAATTTATCAAGTTGATA+AGG + Chr5:107231987-107232006 MsG0580030141.01.T01:three_prime_UTR 25.0%
AAATTCAAGTGTAATCACCA+CGG - Chr5:107231882-107231901 None:intergenic 30.0%
CTGTCAAAATTAATTCAGGA+TGG + Chr5:107231920-107231939 MsG0580030141.01.T01:three_prime_UTR 30.0%
GGAACTGTCAAAATTAATTC+AGG + Chr5:107231916-107231935 MsG0580030141.01.T01:three_prime_UTR 30.0%
TCAACTTGATAAATTGCAAC+AGG - Chr5:107231985-107232004 None:intergenic 30.0%
TTTGACAGTTCCAGAATTAT+CGG - Chr5:107231908-107231927 None:intergenic 30.0%
AAATGTGCAAAGCATGGAAA+AGG - Chr5:107231803-107231822 None:intergenic 35.0%
AGATTGTCAGCTATGGAATT+TGG + Chr5:107232167-107232186 MsG0580030141.01.T01:three_prime_UTR 35.0%
AGGTTAACAAAATCTGGTGT+AGG - Chr5:107231581-107231600 None:intergenic 35.0%
ATTGTCAGCTATGGAATTTG+GGG + Chr5:107232169-107232188 MsG0580030141.01.T01:three_prime_UTR 35.0%
GAATAGAAATGTGCAAAGCA+TGG - Chr5:107231809-107231828 None:intergenic 35.0%
GATTGTCAGCTATGGAATTT+GGG + Chr5:107232168-107232187 MsG0580030141.01.T01:three_prime_UTR 35.0%
GTATCTACCTTTCTTGAAGA+AGG + Chr5:107232452-107232471 MsG0580030141.01.T01:three_prime_UTR 35.0%
TCAGTGAGGTTAACAAAATC+TGG - Chr5:107231587-107231606 None:intergenic 35.0%
TCTTACAAGATTGTCAGCTA+TGG + Chr5:107232160-107232179 MsG0580030141.01.T01:three_prime_UTR 35.0%
TGAATTTCACCCGATAATTC+TGG + Chr5:107231895-107231914 MsG0580030141.01.T01:three_prime_UTR 35.0%
TTGACAGTTCCAGAATTATC+GGG - Chr5:107231907-107231926 None:intergenic 35.0%
TTGTAAGAAGACGAACTATC+TGG - Chr5:107232148-107232167 None:intergenic 35.0%
! CCTTCAATCCACAAAAGTTT+TGG + Chr5:107231649-107231668 MsG0580030141.01.T01:CDS 35.0%
!! AGCTTTGAGTTGGATAAAGA+AGG - Chr5:107231760-107231779 None:intergenic 35.0%
!! CCAAAACTTTTGTGGATTGA+AGG - Chr5:107231652-107231671 None:intergenic 35.0%
!! TAATCTCATCAGCTTTGAGT+TGG - Chr5:107231770-107231789 None:intergenic 35.0%
AACCAAATGTTGCCAACACA+AGG - Chr5:107232198-107232217 None:intergenic 40.0%
AAGGTTCTTCAATGATGCGA+AGG - Chr5:107231741-107231760 None:intergenic 40.0%
ACTGAATTACTCACTGTTGC+TGG + Chr5:107231602-107231621 MsG0580030141.01.T01:CDS 40.0%
CAACAGTGAGTAATTCAGTG+AGG - Chr5:107231601-107231620 None:intergenic 40.0%
CAAGCAAACAACACTGAAGA+AGG + Chr5:107231686-107231705 MsG0580030141.01.T01:CDS 40.0%
GAACTATCTGGTGAAGATGA+AGG - Chr5:107232136-107232155 None:intergenic 40.0%
TTCAGGATGGTCAATCACTA+AGG + Chr5:107231933-107231952 MsG0580030141.01.T01:three_prime_UTR 40.0%
! AACCTTGTGTTGGCAACATT+TGG + Chr5:107232193-107232212 MsG0580030141.01.T01:three_prime_UTR 40.0%
! GAACGTGTCCAAAACTTTTG+TGG - Chr5:107231660-107231679 None:intergenic 40.0%
! TGATAAGGTTTTGCTTCCTG+AGG + Chr5:107232002-107232021 MsG0580030141.01.T01:three_prime_UTR 40.0%
! TTTGCTTCCTGAGGCAATTT+TGG + Chr5:107232011-107232030 MsG0580030141.01.T01:three_prime_UTR 40.0%
!! CTTCTTCAGTGTTGTTTGCT+TGG - Chr5:107231688-107231707 None:intergenic 40.0%
!! GTTGGCAACATTTGGTTTGT+TGG + Chr5:107232201-107232220 MsG0580030141.01.T01:three_prime_UTR 40.0%
!!! CAGTGTTGTTTGCTTGGTTT+TGG - Chr5:107231682-107231701 None:intergenic 40.0%
ACAAAATCTGGTGTAGGTGC+TGG - Chr5:107231575-107231594 None:intergenic 45.0%
ATCAGTGCCAAAATTGCCTC+AGG - Chr5:107232021-107232040 None:intergenic 45.0%
ATGTTGGAGTTGAGGCAGTT+TGG - Chr5:107231856-107231875 None:intergenic 45.0%
CACTGATATTCCACCTGTTC+CGG + Chr5:107232034-107232053 MsG0580030141.01.T01:three_prime_UTR 45.0%
GTAATCACCACGGCAAATGT+TGG - Chr5:107231872-107231891 None:intergenic 45.0%
! AATTTGGGGCAACCTTGTGT+TGG + Chr5:107232183-107232202 MsG0580030141.01.T01:three_prime_UTR 45.0%
! GATGCGAAGGAACTGTCTTT+TGG - Chr5:107231728-107231747 None:intergenic 45.0%
! TCTGTTGCTTCTGAGCTCAT+CGG + Chr5:107231491-107231510 MsG0580030141.01.T01:CDS 45.0%
!! GGCAACATTTGGTTTGTTGG+TGG + Chr5:107232204-107232223 MsG0580030141.01.T01:three_prime_UTR 45.0%
!! TAAAAAATTAATAAATAAAC+TGG + Chr5:107232390-107232409 MsG0580030141.01.T01:three_prime_UTR 5.0%
CACGGCAAATGTTGGAGTTG+AGG - Chr5:107231864-107231883 None:intergenic 50.0%
CTCAACTCCAACATTTGCCG+TGG + Chr5:107231862-107231881 MsG0580030141.01.T01:three_prime_UTR 50.0%
GAGTTGAGGCAGTTTGGCTA+AGG - Chr5:107231850-107231869 None:intergenic 50.0%
! ACGATTTGCTCCCAGCACTT+TGG - Chr5:107232108-107232127 None:intergenic 50.0%
! GTGGCACTCTTGTGATGATG+AGG + Chr5:107232223-107232242 MsG0580030141.01.T01:three_prime_UTR 50.0%
!! AGAGGGTGGACTTACGGTTT+TGG - Chr5:107231523-107231542 None:intergenic 50.0%
ATCAGCTGCAGGCGCAATTC+TGG - Chr5:107232074-107232093 None:intergenic 55.0%
GCAACAGAGTGCAGCTCACA+AGG - Chr5:107231479-107231498 None:intergenic 55.0%
TCAGCTGGAGAATCAGCTGC+AGG - Chr5:107232085-107232104 None:intergenic 55.0%
TCTCCAGCTGACCAAAGTGC+TGG + Chr5:107232094-107232113 MsG0580030141.01.T01:three_prime_UTR 55.0%
TGCAGGCGCAATTCTGGTGT+GGG - Chr5:107232068-107232087 None:intergenic 55.0%
! CTCCAGCTGACCAAAGTGCT+GGG + Chr5:107232095-107232114 MsG0580030141.01.T01:three_prime_UTR 55.0%
CGCAATTCTGGTGTGGGTGC+AGG - Chr5:107232062-107232081 None:intergenic 60.0%
CTCCCAGCACTTTGGTCAGC+TGG - Chr5:107232100-107232119 None:intergenic 60.0%
CTGCAGGCGCAATTCTGGTG+TGG - Chr5:107232069-107232088 None:intergenic 60.0%
!! CTGGTGTAGGTGCTGGTGCT+GGG - Chr5:107231568-107231587 None:intergenic 60.0%
!! TCTGGTGTAGGTGCTGGTGC+TGG - Chr5:107231569-107231588 None:intergenic 60.0%
AGTTGGACTCTCGGGGGGAG+AGG - Chr5:107231541-107231560 None:intergenic 65.0%
CTGGGCCGGAGTTGGACTCT+CGG - Chr5:107231550-107231569 None:intergenic 65.0%
GTTGGACTCTCGGGGGGAGA+GGG - Chr5:107231540-107231559 None:intergenic 65.0%
TCCCCCCGAGAGTCCAACTC+CGG + Chr5:107231542-107231561 MsG0580030141.01.T01:CDS 65.0%
TCTGGTGTGGGTGCAGGAGC+CGG - Chr5:107232056-107232075 None:intergenic 65.0%
TGGGCCGGAGTTGGACTCTC+GGG - Chr5:107231549-107231568 None:intergenic 65.0%
! GGGGGGAGAGGGTGGACTTA+CGG - Chr5:107231529-107231548 None:intergenic 65.0%
!! TGTAGGTGCTGGTGCTGGGC+CGG - Chr5:107231564-107231583 None:intergenic 65.0%
GCCGGAGTTGGACTCTCGGG+GGG - Chr5:107231546-107231565 None:intergenic 70.0%
GGCCGGAGTTGGACTCTCGG+GGG - Chr5:107231547-107231566 None:intergenic 70.0%
GGGCCGGAGTTGGACTCTCG+GGG - Chr5:107231548-107231567 None:intergenic 70.0%
GGTGCAGGAGCCGGAACAGG+TGG - Chr5:107232047-107232066 None:intergenic 70.0%
GTGGGTGCAGGAGCCGGAAC+AGG - Chr5:107232050-107232069 None:intergenic 70.0%
! TGCTGGTGCTGGGCCGGAGT+TGG - Chr5:107231558-107231577 None:intergenic 70.0%
GGACTCTCGGGGGGAGAGGG+TGG - Chr5:107231537-107231556 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr5 gene 107231461 107232471 107231461 ID=MsG0580030141.01;Name=MsG0580030141.01
Chr5 mRNA 107231461 107232471 107231461 ID=MsG0580030141.01.T01;Parent=MsG0580030141.01;Name=MsG0580030141.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|0|1|690|106
Chr5 exon 107231461 107232471 107231461 ID=MsG0580030141.01.T01:exon:14794;Parent=MsG0580030141.01.T01
Chr5 CDS 107231461 107231781 107231461 ID=MsG0580030141.01.T01:cds;Parent=MsG0580030141.01.T01
Chr5 three_prime_UTR 107231782 107232471 107231782 ID=MsG0580030141.01.T01:three_prime_utr;Parent=MsG0580030141.01.T01
Gene Sequence

>MsG0580030141.01.T01

AGGTTCATTGATTTTCCTTGTGAGCTGCACTCTGTTGCTTCTGAGCTCATCGGCATTTGCCAAAACCGTAAGTCCACCCTCTCCCCCCGAGAGTCCAACTCCGGCCCAGCACCAGCACCTACACCAGATTTTGTTAACCTCACTGAATTACTCACTGTTGCTGGTCCATTTCACACTTTTCTTCAATACCTTCAATCCACAAAAGTTTTGGACACGTTCCAAAACCAAGCAAACAACACTGAAGAAGGAATCACTATCTTTGTTCCAAAAGACAGTTCCTTCGCATCATTGAAGAACCTTCTTTATCCAACTCAAAGCTGA

Protein sequence

>MsG0580030141.01.T01

RFIDFPCELHSVASELIGICQNRKSTLSPRESNSGPAPAPTPDFVNLTELLTVAGPFHTFLQYLQSTKVLDTFQNQANNTEEGITIFVPKDSSFASLKNLLYPTQS*