Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047401.01.T01 | XP_013458487.1 | 90.04 | 251 | 15 | 3 | 1 | 247 | 1 | 245 | 9.23E-143 | 410 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047401.01.T01 | F4K956 | 32.759 | 232 | 105 | 9 | 50 | 244 | 156 | 373 | 4.36E-17 | 82.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047401.01.T01 | A0A072UTW6 | 90.040 | 251 | 15 | 3 | 1 | 247 | 1 | 245 | 4.41e-143 | 410 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001591.01 | MsG0880047401.01 | 0.823200 | 1.574150e-53 | 1.346325e-50 |
| MsG0880045438.01 | MsG0880047401.01 | 0.805193 | 1.501987e-49 | 7.896453e-47 |
| MsG0180005625.01 | MsG0880047401.01 | 0.808225 | 3.438481e-50 | 1.955134e-47 |
| MsG0180005639.01 | MsG0880047401.01 | 0.825807 | 3.832399e-54 | 3.530236e-51 |
| MsG0780041742.01 | MsG0880047401.01 | 0.804495 | 2.102193e-49 | 1.085447e-46 |
| MsG0680032279.01 | MsG0880047401.01 | 0.815427 | 9.297459e-52 | 6.408263e-49 |
| MsG0680032305.01 | MsG0880047401.01 | 0.830514 | 2.812987e-55 | 2.970177e-52 |
| MsG0680033447.01 | MsG0880047401.01 | 0.851545 | 8.278257e-61 | 1.681347e-57 |
| MsG0680034769.01 | MsG0880047401.01 | 0.800088 | 1.698338e-48 | 7.837926e-46 |
| MsG0480020433.01 | MsG0880047401.01 | 0.804862 | 1.761811e-49 | 9.184546e-47 |
| MsG0380015486.01 | MsG0880047401.01 | 0.802221 | 6.217541e-49 | 3.028806e-46 |
| MsG0280008165.01 | MsG0880047401.01 | 0.864551 | 1.121345e-64 | 3.534468e-61 |
| MsG0280008171.01 | MsG0880047401.01 | 0.849836 | 2.503437e-60 | 4.807778e-57 |
| MsG0280008172.01 | MsG0880047401.01 | 0.874786 | 5.144025e-68 | 2.345738e-64 |
| MsG0780036509.01 | MsG0880047401.01 | 0.811540 | 6.652199e-51 | 4.128961e-48 |
| MsG0780039432.01 | MsG0880047401.01 | 0.802728 | 4.889145e-49 | 2.412817e-46 |
| MsG0780039847.01 | MsG0880047401.01 | 0.808510 | 2.989931e-50 | 1.712765e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047401.01.T01 | MTR_4g126210 | 90.040 | 251 | 15 | 3 | 1 | 247 | 1 | 245 | 1.12e-146 | 410 |
| MsG0880047401.01.T01 | MTR_4g126170 | 38.783 | 263 | 66 | 10 | 1 | 246 | 5 | 189 | 1.12e-31 | 115 |
| MsG0880047401.01.T01 | MTR_1g016100 | 32.867 | 286 | 141 | 8 | 8 | 245 | 17 | 299 | 2.76e-29 | 112 |
| MsG0880047401.01.T01 | MTR_2g039320 | 32.932 | 249 | 114 | 7 | 7 | 245 | 12 | 217 | 1.63e-24 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047401.01.T01 | AT2G26110 | 37.313 | 268 | 129 | 11 | 6 | 244 | 43 | 300 | 1.24e-30 | 115 |
| MsG0880047401.01.T01 | AT4G26130 | 34.028 | 288 | 129 | 12 | 6 | 244 | 5 | 280 | 2.77e-18 | 82.4 |
| MsG0880047401.01.T01 | AT5G56980 | 32.759 | 232 | 105 | 9 | 50 | 244 | 156 | 373 | 4.45e-18 | 82.8 |
| MsG0880047401.01.T01 | AT5G56980 | 31.548 | 168 | 90 | 6 | 6 | 148 | 5 | 172 | 1.64e-12 | 66.6 |
Find 56 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATTGTAAACCTTGTTAT+AGG | 0.263832 | 8:-85747733 | MsG0880047401.01.T01:CDS |
| CTCTTTACCTCATCGCTGAC+CGG | 0.346958 | 8:+85747298 | None:intergenic |
| CTGCAACAAATGATGGTTCT+TGG | 0.349234 | 8:+85747581 | None:intergenic |
| ACCATCATTTGTTGCAGAAC+CGG | 0.359413 | 8:-85747575 | MsG0880047401.01.T01:CDS |
| GAGGTTGTTGGTGTTGGGTT+TGG | 0.362660 | 8:+85747665 | None:intergenic |
| TGAGTTGAGGTTGTTGGTGT+TGG | 0.367035 | 8:+85747659 | None:intergenic |
| GTTGTAGTGACGGAAGTTAA+AGG | 0.372378 | 8:+85747603 | None:intergenic |
| CGCTGACCGGGTTCAGAGAT+GGG | 0.379247 | 8:+85747311 | None:intergenic |
| CTCCTCGCCGCCGCCGTCGC+TGG | 0.383420 | 8:+85747193 | None:intergenic |
| CTTCACTTCTCAACCGTATC+AGG | 0.388196 | 8:-85747627 | MsG0880047401.01.T01:CDS |
| GAGTTGAGGTTGTTGGTGTT+GGG | 0.397570 | 8:+85747660 | None:intergenic |
| AAGCTGATGATTTCATCAAC+AGG | 0.418680 | 8:-85747129 | MsG0880047401.01.T01:CDS |
| AACGATTGAGTTGAGGTTGT+TGG | 0.427795 | 8:+85747653 | None:intergenic |
| AAGAAGGCCAGCGACGGCGG+CGG | 0.457267 | 8:-85747200 | MsG0880047401.01.T01:CDS |
| TCGCTGACCGGGTTCAGAGA+TGG | 0.480905 | 8:+85747310 | None:intergenic |
| TCCGGTTCTGCAACAAATGA+TGG | 0.484555 | 8:+85747574 | None:intergenic |
| CATGTTGCTATGAAAGTTGT+TGG | 0.489388 | 8:+85747778 | None:intergenic |
| GTTGATTCAGACTCAGACTC+GGG | 0.495280 | 8:+85747427 | None:intergenic |
| TGTTGATTCAGACTCAGACT+CGG | 0.501879 | 8:+85747426 | None:intergenic |
| TTCGTGTTTGTTGAGATTGA+AGG | 0.508523 | 8:+85747480 | None:intergenic |
| GCTCTAGCTTCTCGCTTCAA+TGG | 0.509488 | 8:-85747703 | MsG0880047401.01.T01:CDS |
| TTCGTGTTTGTTGAGATTGA+AGG | 0.512433 | 8:+85747357 | None:intergenic |
| TTCATCTTCTTTGCACGTCG+TGG | 0.520246 | 8:+85747165 | None:intergenic |
| GGAGGAAGAAGATGAAGAAA+CGG | 0.520656 | 8:-85747230 | MsG0880047401.01.T01:CDS |
| AAGAAACGGTGGAGAGAAGA+AGG | 0.523159 | 8:-85747216 | MsG0880047401.01.T01:CDS |
| CGAGCTCGAAAACGTTTGAA+AGG | 0.532874 | 8:-85747252 | MsG0880047401.01.T01:CDS |
| TCTTTACCTCATCGCTGACC+GGG | 0.535702 | 8:+85747299 | None:intergenic |
| GAGAAGAAGGCCAGCGACGG+CGG | 0.537567 | 8:-85747203 | MsG0880047401.01.T01:CDS |
| GGCCAGCGACGGCGGCGGCG+AGG | 0.541710 | 8:-85747195 | MsG0880047401.01.T01:CDS |
| ATAACTCTCTCTAACAAAGA+AGG | 0.544656 | 8:+85747505 | None:intergenic |
| TCGGACTCTGACTCTACCTC+CGG | 0.548761 | 8:+85747556 | None:intergenic |
| AGAAGCAACTACGGTTGCAA+AGG | 0.555330 | 8:-85747102 | MsG0880047401.01.T01:CDS |
| GAGTTGAGGCTGAGTGAACT+CGG | 0.556334 | 8:+85747537 | None:intergenic |
| CTCGAAAACGTTTGAAAGGG+AGG | 0.563627 | 8:-85747248 | MsG0880047401.01.T01:CDS |
| ACAATGATGAAAATAGAAGA+AGG | 0.575111 | 8:+85747748 | None:intergenic |
| GGAAGTTAAAGGACCTGATA+CGG | 0.584536 | 8:+85747614 | None:intergenic |
| GGAGAGAAGAAGGCCAGCGA+CGG | 0.586100 | 8:-85747206 | MsG0880047401.01.T01:CDS |
| ATGTTGCTATGAAAGTTGTT+GGG | 0.590580 | 8:+85747779 | None:intergenic |
| CTGATACGGTTGAGAAGTGA+AGG | 0.593968 | 8:+85747628 | None:intergenic |
| GAGCTCGAAAACGTTTGAAA+GGG | 0.597585 | 8:-85747251 | MsG0880047401.01.T01:CDS |
| GGAAGAAGATGAAGAAACGG+TGG | 0.601259 | 8:-85747227 | MsG0880047401.01.T01:CDS |
| AATCTCAACAAACACGAACC+AGG | 0.605009 | 8:-85747352 | MsG0880047401.01.T01:CDS |
| CAACAACTTTCATAGCAACA+TGG | 0.606436 | 8:-85747777 | MsG0880047401.01.T01:CDS |
| TGAAAACCCATCTCTGAACC+CGG | 0.607759 | 8:-85747317 | MsG0880047401.01.T01:CDS |
| ACAGGTTTAAGAAGCAACTA+CGG | 0.608928 | 8:-85747111 | MsG0880047401.01.T01:CDS |
| GAAGCGAGAAGCTAGAGCTA+TGG | 0.615154 | 8:+85747708 | None:intergenic |
| TAATGTGTCTGTGGATATTG+TGG | 0.622076 | 8:+85747457 | None:intergenic |
| ATGGTTCTTGGTTGTAGTGA+CGG | 0.623543 | 8:+85747593 | None:intergenic |
| ATCATTTGTTGCAGAACCGG+AGG | 0.633321 | 8:-85747572 | MsG0880047401.01.T01:CDS |
| CAGCGATGAGGTAAAGAGCA+AGG | 0.634647 | 8:-85747293 | MsG0880047401.01.T01:CDS |
| ATGACTCGTTGTAACAGAGA+CGG | 0.646476 | 8:+85747382 | None:intergenic |
| GAAGGTGAACGATTGAGTTG+AGG | 0.653353 | 8:+85747646 | None:intergenic |
| TCTGAACCCGGTCAGCGATG+AGG | 0.655711 | 8:-85747305 | MsG0880047401.01.T01:CDS |
| GAGCTATGGTACCTATAACA+AGG | 0.664959 | 8:+85747722 | None:intergenic |
| GGTTGAACGTAATGTGTCTG+TGG | 0.666874 | 8:+85747448 | None:intergenic |
| CGATGAGGTAAAGAGCAAGG+TGG | 0.704311 | 8:-85747290 | MsG0880047401.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAATGATGAAAATAGAAGA+AGG | + | Chr8:85747105-85747124 | None:intergenic | 25.0% |
| !! | ATCATTGTAAACCTTGTTAT+AGG | - | Chr8:85747117-85747136 | MsG0880047401.01.T01:CDS | 25.0% |
| ATAACTCTCTCTAACAAAGA+AGG | + | Chr8:85747348-85747367 | None:intergenic | 30.0% | |
| ATGTTGCTATGAAAGTTGTT+GGG | + | Chr8:85747074-85747093 | None:intergenic | 30.0% | |
| AAGCTGATGATTTCATCAAC+AGG | - | Chr8:85747721-85747740 | MsG0880047401.01.T01:CDS | 35.0% | |
| ACAGGTTTAAGAAGCAACTA+CGG | - | Chr8:85747739-85747758 | MsG0880047401.01.T01:CDS | 35.0% | |
| CAACAACTTTCATAGCAACA+TGG | - | Chr8:85747073-85747092 | MsG0880047401.01.T01:CDS | 35.0% | |
| CATGTTGCTATGAAAGTTGT+TGG | + | Chr8:85747075-85747094 | None:intergenic | 35.0% | |
| TAATGTGTCTGTGGATATTG+TGG | + | Chr8:85747396-85747415 | None:intergenic | 35.0% | |
| ! | TTCGTGTTTGTTGAGATTGA+AGG | + | Chr8:85747373-85747392 | None:intergenic | 35.0% |
| !!! | TGTTGGGTTTGGTCATTTTT+GGG | + | Chr8:85747177-85747196 | None:intergenic | 35.0% |
| AATCTCAACAAACACGAACC+AGG | - | Chr8:85747375-85747394 | MsG0880047401.01.T01:CDS | 40.0% | |
| ATGACTCGTTGTAACAGAGA+CGG | + | Chr8:85747471-85747490 | None:intergenic | 40.0% | |
| ATGGTTCTTGGTTGTAGTGA+CGG | + | Chr8:85747260-85747279 | None:intergenic | 40.0% | |
| CTGCAACAAATGATGGTTCT+TGG | + | Chr8:85747272-85747291 | None:intergenic | 40.0% | |
| GAGCTATGGTACCTATAACA+AGG | + | Chr8:85747131-85747150 | None:intergenic | 40.0% | |
| GAGCTCGAAAACGTTTGAAA+GGG | - | Chr8:85747599-85747618 | MsG0880047401.01.T01:CDS | 40.0% | |
| GGAAGTTAAAGGACCTGATA+CGG | + | Chr8:85747239-85747258 | None:intergenic | 40.0% | |
| GGAGGAAGAAGATGAAGAAA+CGG | - | Chr8:85747620-85747639 | MsG0880047401.01.T01:CDS | 40.0% | |
| ! | ACCATCATTTGTTGCAGAAC+CGG | - | Chr8:85747275-85747294 | MsG0880047401.01.T01:CDS | 40.0% |
| ! | GTTGTAGTGACGGAAGTTAA+AGG | + | Chr8:85747250-85747269 | None:intergenic | 40.0% |
| !! | AACGATTGAGTTGAGGTTGT+TGG | + | Chr8:85747200-85747219 | None:intergenic | 40.0% |
| !! | GTGTTGGGTTTGGTCATTTT+TGG | + | Chr8:85747178-85747197 | None:intergenic | 40.0% |
| !! | TGTTGATTCAGACTCAGACT+CGG | + | Chr8:85747427-85747446 | None:intergenic | 40.0% |
| AAGAAACGGTGGAGAGAAGA+AGG | - | Chr8:85747634-85747653 | MsG0880047401.01.T01:CDS | 45.0% | |
| AGAAGCAACTACGGTTGCAA+AGG | - | Chr8:85747748-85747767 | MsG0880047401.01.T01:CDS | 45.0% | |
| CGAGCTCGAAAACGTTTGAA+AGG | - | Chr8:85747598-85747617 | MsG0880047401.01.T01:CDS | 45.0% | |
| CTCGAAAACGTTTGAAAGGG+AGG | - | Chr8:85747602-85747621 | MsG0880047401.01.T01:CDS | 45.0% | |
| CTGATACGGTTGAGAAGTGA+AGG | + | Chr8:85747225-85747244 | None:intergenic | 45.0% | |
| CTTCACTTCTCAACCGTATC+AGG | - | Chr8:85747223-85747242 | MsG0880047401.01.T01:CDS | 45.0% | |
| GGAAGAAGATGAAGAAACGG+TGG | - | Chr8:85747623-85747642 | MsG0880047401.01.T01:CDS | 45.0% | |
| GGTTGAACGTAATGTGTCTG+TGG | + | Chr8:85747405-85747424 | None:intergenic | 45.0% | |
| TCCGGTTCTGCAACAAATGA+TGG | + | Chr8:85747279-85747298 | None:intergenic | 45.0% | |
| TGAAAACCCATCTCTGAACC+CGG | - | Chr8:85747533-85747552 | MsG0880047401.01.T01:CDS | 45.0% | |
| TTCATCTTCTTTGCACGTCG+TGG | + | Chr8:85747688-85747707 | None:intergenic | 45.0% | |
| ! | ATCATTTGTTGCAGAACCGG+AGG | - | Chr8:85747278-85747297 | MsG0880047401.01.T01:CDS | 45.0% |
| ! | GAAGGTGAACGATTGAGTTG+AGG | + | Chr8:85747207-85747226 | None:intergenic | 45.0% |
| ! | GAAGGTTTTCGAACGAGTTG+AGG | + | Chr8:85747330-85747349 | None:intergenic | 45.0% |
| ! | GAGTTGAGGTTGTTGGTGTT+GGG | + | Chr8:85747193-85747212 | None:intergenic | 45.0% |
| ! | GTTGATTCAGACTCAGACTC+GGG | + | Chr8:85747426-85747445 | None:intergenic | 45.0% |
| ! | TGAGTTGAGGTTGTTGGTGT+TGG | + | Chr8:85747194-85747213 | None:intergenic | 45.0% |
| !! | AGAGATGGGTTTTCAGCTTC+GGG | + | Chr8:85747528-85747547 | None:intergenic | 45.0% |
| !! | CAGAGATGGGTTTTCAGCTT+CGG | + | Chr8:85747529-85747548 | None:intergenic | 45.0% |
| !!! | TGGGTTTGGTCATTTTTGGG+TGG | + | Chr8:85747174-85747193 | None:intergenic | 45.0% |
| CAGCGATGAGGTAAAGAGCA+AGG | - | Chr8:85747557-85747576 | MsG0880047401.01.T01:CDS | 50.0% | |
| CGATGAGGTAAAGAGCAAGG+TGG | - | Chr8:85747560-85747579 | MsG0880047401.01.T01:CDS | 50.0% | |
| CTCTTTACCTCATCGCTGAC+CGG | + | Chr8:85747555-85747574 | None:intergenic | 50.0% | |
| GAAGCGAGAAGCTAGAGCTA+TGG | + | Chr8:85747145-85747164 | None:intergenic | 50.0% | |
| GAGTTGAGGCTGAGTGAACT+CGG | + | Chr8:85747316-85747335 | None:intergenic | 50.0% | |
| TCTTTACCTCATCGCTGACC+GGG | + | Chr8:85747554-85747573 | None:intergenic | 50.0% | |
| ! | GACGGTTTTCGAACGAGTTG+AGG | + | Chr8:85747453-85747472 | None:intergenic | 50.0% |
| ! | GCTCTAGCTTCTCGCTTCAA+TGG | - | Chr8:85747147-85747166 | MsG0880047401.01.T01:CDS | 50.0% |
| !! | GAGGTTGTTGGTGTTGGGTT+TGG | + | Chr8:85747188-85747207 | None:intergenic | 50.0% |
| !!! | TTTTCAGCTTCGGGTTGTCC+TGG | + | Chr8:85747519-85747538 | None:intergenic | 50.0% |
| TCGGACTCTGACTCTACCTC+CGG | + | Chr8:85747297-85747316 | None:intergenic | 55.0% | |
| CGCTGACCGGGTTCAGAGAT+GGG | + | Chr8:85747542-85747561 | None:intergenic | 60.0% | |
| GGAGAGAAGAAGGCCAGCGA+CGG | - | Chr8:85747644-85747663 | MsG0880047401.01.T01:CDS | 60.0% | |
| TCGCTGACCGGGTTCAGAGA+TGG | + | Chr8:85747543-85747562 | None:intergenic | 60.0% | |
| TCTGAACCCGGTCAGCGATG+AGG | - | Chr8:85747545-85747564 | MsG0880047401.01.T01:CDS | 60.0% | |
| GAGAAGAAGGCCAGCGACGG+CGG | - | Chr8:85747647-85747666 | MsG0880047401.01.T01:CDS | 65.0% | |
| AAGAAGGCCAGCGACGGCGG+CGG | - | Chr8:85747650-85747669 | MsG0880047401.01.T01:CDS | 70.0% | |
| ! | CTCCTCGCCGCCGCCGTCGC+TGG | + | Chr8:85747660-85747679 | None:intergenic | 85.0% |
| ! | GGCCAGCGACGGCGGCGGCG+AGG | - | Chr8:85747655-85747674 | MsG0880047401.01.T01:CDS | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 85747063 | 85747809 | 85747063 | ID=MsG0880047401.01;Name=MsG0880047401.01 |
| Chr8 | mRNA | 85747063 | 85747809 | 85747063 | ID=MsG0880047401.01.T01;Parent=MsG0880047401.01;Name=MsG0880047401.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|248 |
| Chr8 | exon | 85747063 | 85747809 | 85747063 | ID=MsG0880047401.01.T01:exon:34527;Parent=MsG0880047401.01.T01 |
| Chr8 | CDS | 85747063 | 85747809 | 85747063 | ID=MsG0880047401.01.T01:cds;Parent=MsG0880047401.01.T01 |
| Gene Sequence |
| Protein sequence |
