AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0005030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0005030 MtrunA17_Chr1g0151701 75.758 66 16 0 10 75 120 185 1.06e-35 120
Msa0005030 MtrunA17_Chr1g0151721 82.812 64 11 0 11 74 290 353 1.39e-35 124
Msa0005030 MtrunA17_Chr3g0088841 73.333 60 16 0 11 70 314 373 8.86e-26 98.6
Msa0005030 MtrunA17_Chr3g0088561 57.971 69 22 2 10 75 115 179 3.73e-21 82.8
Msa0005030 MtrunA17_Chr2g0303111 65.079 63 13 1 11 73 319 372 3.75e-21 85.9
Msa0005030 MtrunA17_Chr3g0088751 58.462 65 26 1 11 75 317 380 4.59e-19 80.1
Msa0005030 MtrunA17_Chr1g0170781 62.069 58 20 1 11 68 341 396 1.35e-18 79.0
Msa0005030 MtrunA17_Chr3g0127311 56.250 64 26 1 11 74 330 391 3.31e-16 72.4
Msa0005030 MtrunA17_Chr3g0127321 52.941 68 30 1 11 78 371 436 6.15e-16 71.6
Msa0005030 MtrunA17_Chr3g0088951 55.738 61 24 1 11 71 302 359 6.83e-15 68.6
Msa0005030 MtrunA17_Chr6g0457531 56.897 58 20 2 11 68 313 365 1.49e-14 67.8
Msa0005030 MtrunA17_Chr3g0088721 52.381 63 28 2 11 71 319 381 1.88e-14 67.4
Msa0005030 MtrunA17_Chr3g0088831 50.000 58 27 1 11 68 365 420 2.16e-13 64.3
Msa0005030 MtrunA17_Chr8g0392121 50.000 60 28 1 11 70 118 175 3.14e-13 62.0
Msa0005030 MtrunA17_Chr3g0088991 54.098 61 18 3 11 68 286 339 5.48e-13 63.2
Msa0005030 MtrunA17_Chr3g0088531 46.875 64 31 2 10 72 315 376 5.65e-13 63.2
Msa0005030 MtrunA17_Chr3g0088631 53.226 62 26 2 9 70 329 387 7.40e-13 62.8
Msa0005030 MtrunA17_Chr3g0089001 54.098 61 18 3 11 68 330 383 9.80e-13 62.4
Msa0005030 MtrunA17_Chr3g0092331 42.029 69 38 1 9 77 123 189 3.23e-12 59.7
Msa0005030 MtrunA17_Chr3g0089051 45.455 66 29 2 10 68 367 432 3.68e-12 60.8
Msa0005030 MtrunA17_Chr4g0010441 43.939 66 35 1 6 71 25 88 6.97e-12 57.0
Msa0005030 MtrunA17_Chr3g0088601 53.333 60 25 2 11 70 343 399 9.11e-12 59.7
Msa0005030 MtrunA17_Chr7g0214521 43.333 60 34 0 11 70 306 365 1.21e-11 59.3
Msa0005030 MtrunA17_Chr3g0088921 48.333 60 28 2 11 70 339 395 1.35e-11 59.3
Msa0005030 MtrunA17_Chr1g0146361 48.333 60 24 1 9 68 29 81 5.64e-11 53.9
Msa0005030 MtrunA17_Chr6g0457501 48.276 58 30 0 11 68 323 380 5.97e-11 57.4
Msa0005030 MtrunA17_Chr3g0088541 43.333 60 31 2 10 68 306 363 8.39e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 87 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTTTATGTACTTGAATT+AGG 0.191945 1_1:+7466599 Msa0005030:five_prime_UTR
AAACCAACGGAGATGAATTT+TGG 0.212262 1_1:+7466762 Msa0005030:five_prime_UTR
ATGGGTTGATTTGATGAGTT+TGG 0.252099 1_1:+7467081 Msa0005030:five_prime_UTR
TTAACCACAAGTTCATATTT+AGG 0.261634 1_1:-7466920 None:intergenic
ATTTGATGAGTTTGGGAGAT+AGG 0.289011 1_1:+7467089 Msa0005030:five_prime_UTR
GAGGAGTGGGTGTAAGAGTT+TGG 0.312170 1_1:-7466525 None:intergenic
AAATATGAACTTGTGGTTAA+TGG 0.323243 1_1:+7466923 Msa0005030:five_prime_UTR
GTAAAGATCTGTAATGATAT+TGG 0.331886 1_1:+7469761 Msa0005030:CDS
GTGGACATTTATGAGTGTTT+AGG 0.334870 1_1:+7466992 Msa0005030:five_prime_UTR
GATCGCATGACTTCGATAAC+TGG 0.340536 1_1:+7466888 Msa0005030:five_prime_UTR
CCAATTCCATCAATCATATA+AGG 0.345857 1_1:-7466842 None:intergenic
CGGACAATTATTGTTGGACT+TGG 0.356140 1_1:+7466866 Msa0005030:five_prime_UTR
TTCACCTTTGTCTGATTGTC+CGG 0.356602 1_1:+7467271 Msa0005030:CDS
GTTGTTGATGGGAGATTGAT+AGG 0.367917 1_1:-7466399 None:intergenic
TGGAAACTTGAGGGTTAGAT+TGG 0.369886 1_1:-7466372 None:intergenic
ATCAGATGTAGAAGCTCCTT+TGG 0.370393 1_1:-7466266 None:intergenic
TTTCAACGAGACTGATTTCT+TGG 0.370571 1_1:-7466741 None:intergenic
GCAAGATAACATACTCTGTT+AGG 0.372490 1_1:-7467347 None:intergenic
TTCAGATTTGTGTTTCTAAA+GGG 0.375910 1_1:+7467153 Msa0005030:CDS
CTTCAGATTTGTGTTTCTAA+AGG 0.383280 1_1:+7467152 Msa0005030:CDS
CAGAACAAGTTAAAATATTC+CGG 0.384086 1_1:-7467290 None:intergenic
TTGAGGGTTAGATTGGGAAT+TGG 0.401176 1_1:-7466365 None:intergenic
CCTTATATGATTGATGGAAT+TGG 0.407615 1_1:+7466842 Msa0005030:five_prime_UTR
TTGATGAGAAGGAGAGTGAA+TGG 0.411083 1_1:+7467062 Msa0005030:five_prime_UTR
GATGAGGAAGAAAGAGCGTT+TGG 0.413225 1_1:-7466444 None:intergenic
TACTGATATGTCTATGTACT+TGG 0.428125 1_1:+7466793 Msa0005030:five_prime_UTR
CGAGTTCAACGTCGAGTCGT+TGG 0.433682 1_1:-7466292 None:intergenic
TCAGGGTAAACATAGTCATA+TGG 0.438455 1_1:-7466656 None:intergenic
TTGTTGATGGGAGATTGATA+GGG 0.442668 1_1:-7466398 None:intergenic
GAGTTCAACGTCGAGTCGTT+GGG 0.443077 1_1:-7466291 None:intergenic
TGTATCTTTGACTTGGATCA+AGG 0.447583 1_1:+7467242 Msa0005030:CDS
GGGCGGCCTTATATGATTGA+TGG 0.452306 1_1:+7466836 Msa0005030:five_prime_UTR
AACCACCACTTCAACGCCCT+TGG 0.454393 1_1:+7466468 Msa0005030:five_prime_UTR
GGTTAGATTGGGAATTGGTA+AGG 0.460418 1_1:-7466360 None:intergenic
TGGGTTGATTTGATGAGTTT+GGG 0.463445 1_1:+7467082 Msa0005030:five_prime_UTR
TGTGTTTAGGTTTGATGAGA+AGG 0.463451 1_1:+7467051 Msa0005030:five_prime_UTR
AAATTGAGTGGTGTTGTTGA+TGG 0.466840 1_1:-7466411 None:intergenic
TTTGATGAGTTTGGGAGATA+GGG 0.474756 1_1:+7467090 Msa0005030:five_prime_UTR
ATAGGGGTGTGGAAACTTGA+GGG 0.481501 1_1:-7466381 None:intergenic
ATTGGTCGGACAATTATTGT+TGG 0.482444 1_1:+7466860 Msa0005030:five_prime_UTR
TCAGATGTAGAAGCTCCTTT+GGG 0.486819 1_1:-7466265 None:intergenic
CATCAATGGCAGAGTCAGAC+TGG 0.493551 1_1:+7466234 Msa0005030:five_prime_UTR
TCTGCCATTGATGTTGCTGT+TGG 0.493680 1_1:-7466224 None:intergenic
ACACCAAAATTCATCTCCGT+TGG 0.512605 1_1:-7466765 None:intergenic
TCCGATCAACTTGTTCCGCA+TGG 0.522258 1_1:+7466327 Msa0005030:five_prime_UTR
TGGGAACTGTATCTTTGACT+TGG 0.526556 1_1:+7467235 Msa0005030:CDS
AACCAAGGGCGTTGAAGTGG+TGG 0.538102 1_1:-7466470 None:intergenic
ATGGGAGATTGATAGGGGTG+TGG 0.538525 1_1:-7466392 None:intergenic
TATGAACTTGTGGTTAATGG+TGG 0.543129 1_1:+7466926 Msa0005030:five_prime_UTR
GATAGGGGTGTGGAAACTTG+AGG 0.545526 1_1:-7466382 None:intergenic
TGATGAGAAGGAGAGTGAAT+GGG 0.545602 1_1:+7467063 Msa0005030:five_prime_UTR
AATTGAGTGGTGTTGTTGAT+GGG 0.546505 1_1:-7466410 None:intergenic
GGAAACTTGAGGGTTAGATT+GGG 0.553565 1_1:-7466371 None:intergenic
TGAGGGCGCGTTGCTGCGAG+TGG 0.554191 1_1:+7466973 Msa0005030:five_prime_UTR
TACACCAACAGCAACATCAA+TGG 0.554968 1_1:+7466220 None:intergenic
ATCAACCAAGGGCGTTGAAG+TGG 0.559025 1_1:-7466473 None:intergenic
TGAACTTGTGGTTAATGGTG+GGG 0.569319 1_1:+7466928 Msa0005030:five_prime_UTR
TGTTGATGGGAGATTGATAG+GGG 0.579514 1_1:-7466397 None:intergenic
GAACTTGTGGTTAATGGTGG+GGG 0.582058 1_1:+7466929 Msa0005030:five_prime_UTR
ATGAACTTGTGGTTAATGGT+GGG 0.584883 1_1:+7466927 Msa0005030:five_prime_UTR
GAGTAATTCTGATCAACCAA+GGG 0.589964 1_1:-7466484 None:intergenic
GTTGAAGTGGTGGTTTGATG+AGG 0.590656 1_1:-7466460 None:intergenic
ATGATTGATACGAGAGGAGT+GGG 0.590980 1_1:-7466538 None:intergenic
TTGTCGATCCACTCTGTAGG+TGG 0.596783 1_1:-7467314 None:intergenic
ACCACCTAAATATGAACTTG+TGG 0.597935 1_1:+7466916 Msa0005030:five_prime_UTR
AGACTGGTCTTCACTCCCAA+AGG 0.598232 1_1:+7466250 Msa0005030:five_prime_UTR
TCACTAAAATCGAGAGCGTG+AGG 0.598776 1_1:-7466578 None:intergenic
AGAGCGTGAGGGAAAAGAAG+AGG 0.600055 1_1:-7466566 None:intergenic
ACTGATATGTCTATGTACTT+GGG 0.602971 1_1:+7466794 Msa0005030:five_prime_UTR
ACCACAAGTTCATATTTAGG+TGG 0.605610 1_1:-7466917 None:intergenic
TGAGTAATTCTGATCAACCA+AGG 0.612564 1_1:-7466485 None:intergenic
AAAATTCTTAGTGGAGAGTG+AGG 0.612751 1_1:+7466955 Msa0005030:five_prime_UTR
CACTAAAATCGAGAGCGTGA+GGG 0.616468 1_1:-7466577 None:intergenic
CATTTGTCGATCCACTCTGT+AGG 0.617177 1_1:-7467317 None:intergenic
GATGAGATGATTGATACGAG+AGG 0.619882 1_1:-7466544 None:intergenic
TCAGTCTCGTTGAAAACCAA+CGG 0.621836 1_1:+7466749 Msa0005030:five_prime_UTR
GCCATGCGGAACAAGTTGAT+CGG 0.628441 1_1:-7466328 None:intergenic
GGAGAGAGAGTGAAATTGAG+TGG 0.633283 1_1:-7466423 None:intergenic
ATATGATTGATGGAATTGGT+CGG 0.636318 1_1:+7466846 Msa0005030:five_prime_UTR
ATAAACGTGCCAATAACAAG+AGG 0.645995 1_1:-7466713 None:intergenic
AAATTCTTAGTGGAGAGTGA+GGG 0.668174 1_1:+7466956 Msa0005030:five_prime_UTR
AGAATTACTCAAAACCCTAA+CGG 0.674771 1_1:+7466497 Msa0005030:five_prime_UTR
TATTCCGGACAATCAGACAA+AGG 0.675553 1_1:-7467275 None:intergenic
TGTTCTGGCCACCTACAGAG+TGG 0.683477 1_1:+7467306 Msa0005030:CDS
GATGATTGATACGAGAGGAG+TGG 0.702452 1_1:-7466539 None:intergenic
ATAGCACTAACAGACCCAAG+AGG 0.719141 1_1:+7469789 Msa0005030:CDS
TAAGGAAGAAGAGCGCCATG+CGG 0.759001 1_1:-7466342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAAAAGTTATATATAAA+CGG + chr1_1:7467677-7467696 Msa0005030:intron 10.0%
!! AATTAGCTAAGTAATATATT+TGG - chr1_1:7467595-7467614 None:intergenic 15.0%
!!! AATAATCTCATTTTTAAGTT+CGG - chr1_1:7468075-7468094 None:intergenic 15.0%
!! CAATCTAAATATAAAATAAG+AGG + chr1_1:7468500-7468519 Msa0005030:intron 15.0%
!!! AATTTTTTTGATGTCTTATA+TGG - chr1_1:7469520-7469539 None:intergenic 15.0%
!! AAAATACAAAAATAACAGAA+GGG - chr1_1:7469712-7469731 None:intergenic 15.0%
!! AAAAATACAAAAATAACAGA+AGG - chr1_1:7469713-7469732 None:intergenic 15.0%
!!! TTTTAAGGATTAATGTGTTT+AGG + chr1_1:7467038-7467057 Msa0005030:five_prime_UTR 20.0%
!! AATTGTAAAAACATGCAATA+TGG + chr1_1:7467215-7467234 Msa0005030:CDS 20.0%
!! ATTGTAAAAACATGCAATAT+GGG + chr1_1:7467216-7467235 Msa0005030:CDS 20.0%
!! ATATTACTTAGCTAATTGTT+AGG + chr1_1:7467598-7467617 Msa0005030:intron 20.0%
!! TGTTAGGAATCTTAAATATT+TGG + chr1_1:7467614-7467633 Msa0005030:intron 20.0%
!! ATATATAAACGGTTAAGATT+TGG + chr1_1:7467688-7467707 Msa0005030:intron 20.0%
!!! CTTTTAATTGTTGATTTGTT+GGG - chr1_1:7467813-7467832 None:intergenic 20.0%
!!! TCTTTTAATTGTTGATTTGT+TGG - chr1_1:7467814-7467833 None:intergenic 20.0%
!! CAATTAAAAGAGTAAATTAG+AGG + chr1_1:7467823-7467842 Msa0005030:intron 20.0%
!!! AATGGATTATAGAAAAATGA+TGG - chr1_1:7467951-7467970 None:intergenic 20.0%
!!! GATAATAAATGGCTTTTTAA+TGG - chr1_1:7467969-7467988 None:intergenic 20.0%
!! AGAAGAAAAAGGATAATAAA+TGG - chr1_1:7467980-7467999 None:intergenic 20.0%
!!! ATAATCTCATTTTTAAGTTC+GGG - chr1_1:7468074-7468093 None:intergenic 20.0%
!! AATAACATGCCAAAAAAATA+GGG - chr1_1:7468120-7468139 None:intergenic 20.0%
!! AAATAACATGCCAAAAAAAT+AGG - chr1_1:7468121-7468140 None:intergenic 20.0%
!! ATCAAAATAGTTTAAGAAAC+TGG + chr1_1:7468175-7468194 Msa0005030:intron 20.0%
!! AGAATGTTTGTATATAAGTT+GGG - chr1_1:7468473-7468492 None:intergenic 20.0%
!! TAGAATGTTTGTATATAAGT+TGG - chr1_1:7468474-7468493 None:intergenic 20.0%
!! TAACAATATGTTCAAAGATT+TGG + chr1_1:7468638-7468657 Msa0005030:intron 20.0%
!! TAAATAAACAAGAAACAAAG+AGG - chr1_1:7468875-7468894 None:intergenic 20.0%
!! ATATATACAAATGAAGATGA+AGG - chr1_1:7468914-7468933 None:intergenic 20.0%
!!! AAGTAACAAATCTTTTTGTT+AGG - chr1_1:7469088-7469107 None:intergenic 20.0%
!!! AAAGATTTGTTACTTTATAC+AGG + chr1_1:7469093-7469112 Msa0005030:intron 20.0%
!!! AAGATTTGTTACTTTATACA+GGG + chr1_1:7469094-7469113 Msa0005030:intron 20.0%
!! AAATACAAAAATAACAGAAG+GGG - chr1_1:7469711-7469730 None:intergenic 20.0%
! GAACTTTATGTACTTGAATT+AGG + chr1_1:7466599-7466618 Msa0005030:five_prime_UTR 25.0%
! TTAACCACAAGTTCATATTT+AGG - chr1_1:7466923-7466942 None:intergenic 25.0%
! AAATATGAACTTGTGGTTAA+TGG + chr1_1:7466923-7466942 Msa0005030:five_prime_UTR 25.0%
!!! TGTTTAGGTTTTGATTACTT+CGG + chr1_1:7467007-7467026 Msa0005030:five_prime_UTR 25.0%
!! TTCAGATTTGTGTTTCTAAA+GGG + chr1_1:7467153-7467172 Msa0005030:CDS 25.0%
! CAGAACAAGTTAAAATATTC+CGG - chr1_1:7467293-7467312 None:intergenic 25.0%
! AAGATTATCTAATCTGACAA+CGG - chr1_1:7467390-7467409 None:intergenic 25.0%
! ACACAATCGTAATAATAACT+AGG - chr1_1:7467479-7467498 None:intergenic 25.0%
!!! TCTTCTTTCTTTGTTACTTT+TGG + chr1_1:7467541-7467560 Msa0005030:intron 25.0%
! TATGTGTTTAGCAACAAATT+TGG + chr1_1:7467568-7467587 Msa0005030:intron 25.0%
!! ACTTTTCAAATATATGCTCA+AGG - chr1_1:7467667-7467686 None:intergenic 25.0%
! TAGTTATAATGGTTCAGATT+TGG - chr1_1:7467767-7467786 None:intergenic 25.0%
! ATAACATGCCAAAAAAATAG+GGG - chr1_1:7468119-7468138 None:intergenic 25.0%
!! ATAGCAAGAGACATTTTTAA+GGG - chr1_1:7468218-7468237 None:intergenic 25.0%
! AACCTCTAATGCTTTAATTA+TGG - chr1_1:7468411-7468430 None:intergenic 25.0%
!! TAATTAAAGCATTAGAGGTT+AGG + chr1_1:7468411-7468430 Msa0005030:intron 25.0%
! GAATGTTTGTATATAAGTTG+GGG - chr1_1:7468472-7468491 None:intergenic 25.0%
! TGAAAGAAATAAGAAGACAT+GGG + chr1_1:7469122-7469141 Msa0005030:intron 25.0%
! TTTGCACAAAAAGTAACAAT+TGG + chr1_1:7469160-7469179 Msa0005030:intron 25.0%
! GAAAAACAGAGAAATAAAGA+GGG - chr1_1:7469202-7469221 None:intergenic 25.0%
! AGAAAAACAGAGAAATAAAG+AGG - chr1_1:7469203-7469222 None:intergenic 25.0%
!! ATTTTACTAGGAAAATACCT+AGG - chr1_1:7469414-7469433 None:intergenic 25.0%
!! ACATTTAAGAGGATTTTACT+AGG - chr1_1:7469426-7469445 None:intergenic 25.0%
! TTGAGAAAAAACAGTAAGAT+TGG - chr1_1:7469613-7469632 None:intergenic 25.0%
!!! AATCTTACTGTTTTTTCTCA+AGG + chr1_1:7469612-7469631 Msa0005030:intron 25.0%
!!! AAGAGCATTTCAATGTTTAA+GGG - chr1_1:7469655-7469674 None:intergenic 25.0%
!!! TAAGAGCATTTCAATGTTTA+AGG - chr1_1:7469656-7469675 None:intergenic 25.0%
! GTGAAAAGACTAAGAAAAAT+CGG - chr1_1:7469689-7469708 None:intergenic 25.0%
! GTAAAGATCTGTAATGATAT+TGG + chr1_1:7469761-7469780 Msa0005030:CDS 25.0%
AGAATTACTCAAAACCCTAA+CGG + chr1_1:7466497-7466516 Msa0005030:five_prime_UTR 30.0%
! GAAAAAAAGCCTCTTGTTAT+TGG + chr1_1:7466704-7466723 Msa0005030:five_prime_UTR 30.0%
TACTGATATGTCTATGTACT+TGG + chr1_1:7466793-7466812 Msa0005030:five_prime_UTR 30.0%
ACTGATATGTCTATGTACTT+GGG + chr1_1:7466794-7466813 Msa0005030:five_prime_UTR 30.0%
CCAATTCCATCAATCATATA+AGG - chr1_1:7466845-7466864 None:intergenic 30.0%
CCTTATATGATTGATGGAAT+TGG + chr1_1:7466842-7466861 Msa0005030:five_prime_UTR 30.0%
ATATGATTGATGGAATTGGT+CGG + chr1_1:7466846-7466865 Msa0005030:five_prime_UTR 30.0%
! ACTTCGGTAAGAATGTTTTA+AGG + chr1_1:7467023-7467042 Msa0005030:five_prime_UTR 30.0%
! CTTCAGATTTGTGTTTCTAA+AGG + chr1_1:7467152-7467171 Msa0005030:CDS 30.0%
!!! CGGAATATTTTAACTTGTTC+TGG + chr1_1:7467291-7467310 Msa0005030:CDS 30.0%
ATCGTAATAATAACTAGGCA+TGG - chr1_1:7467474-7467493 None:intergenic 30.0%
CAAAGAAAGAAGACAACAAA+CGG - chr1_1:7467534-7467553 None:intergenic 30.0%
! TTCGGATCGATTAGTTATAA+TGG - chr1_1:7467778-7467797 None:intergenic 30.0%
ATTATAACTAATCGATCCGA+AGG + chr1_1:7467777-7467796 Msa0005030:intron 30.0%
AGAAATCCAGAAAACAACAA+AGG - chr1_1:7467877-7467896 None:intergenic 30.0%
!! TTATAGAAAAATGATGGTGG+TGG - chr1_1:7467945-7467964 None:intergenic 30.0%
GGATTATAGAAAAATGATGG+TGG - chr1_1:7467948-7467967 None:intergenic 30.0%
ATTTGGATCTTACTGCTAAA+AGG - chr1_1:7468012-7468031 None:intergenic 30.0%
TAGCAGTAAGATCCAAATAT+AGG + chr1_1:7468014-7468033 Msa0005030:intron 30.0%
AGCAGTAAGATCCAAATATA+GGG + chr1_1:7468015-7468034 Msa0005030:intron 30.0%
!!! AGTGTTTACCCCTATTTTTT+TGG + chr1_1:7468108-7468127 Msa0005030:intron 30.0%
! GATAGCAAGAGACATTTTTA+AGG - chr1_1:7468219-7468238 None:intergenic 30.0%
AGGTATATTTGAGTATACAC+CGG + chr1_1:7468295-7468314 Msa0005030:intron 30.0%
! TCGTAATAATAACTTGCCAA+TGG - chr1_1:7468327-7468346 None:intergenic 30.0%
AAGTTTCTAACAATGATGCT+GGG + chr1_1:7468346-7468365 Msa0005030:intron 30.0%
TCAGTTTAAATCTGAGCAAT+GGG - chr1_1:7468382-7468401 None:intergenic 30.0%
TTCAGTTTAAATCTGAGCAA+TGG - chr1_1:7468383-7468402 None:intergenic 30.0%
! CACCATAATTAAAGCATTAG+AGG + chr1_1:7468406-7468425 Msa0005030:intron 30.0%
AATCAATTATCACATGACCA+TGG + chr1_1:7468538-7468557 Msa0005030:intron 30.0%
AAAAAAATGCTAGTACCTCT+AGG + chr1_1:7468684-7468703 Msa0005030:intron 30.0%
AAAAAATGCTAGTACCTCTA+GGG + chr1_1:7468685-7468704 Msa0005030:intron 30.0%
GTGAAAGAAATAAGAAGACA+TGG + chr1_1:7469121-7469140 Msa0005030:intron 30.0%
GAAAGAAATAAGAAGACATG+GGG + chr1_1:7469123-7469142 Msa0005030:intron 30.0%
TCTATCACACATCTTTAGAT+AGG + chr1_1:7469585-7469604 Msa0005030:intron 30.0%
! TTGTTGATGGGAGATTGATA+GGG - chr1_1:7466401-7466420 None:intergenic 35.0%
!! AATTGAGTGGTGTTGTTGAT+GGG - chr1_1:7466413-7466432 None:intergenic 35.0%
!! AAATTGAGTGGTGTTGTTGA+TGG - chr1_1:7466414-7466433 None:intergenic 35.0%
GAGTAATTCTGATCAACCAA+GGG - chr1_1:7466487-7466506 None:intergenic 35.0%
TGAGTAATTCTGATCAACCA+AGG - chr1_1:7466488-7466507 None:intergenic 35.0%
!!! AGAGTTTGGTTTTACCGTTA+GGG - chr1_1:7466514-7466533 None:intergenic 35.0%
!!! AAGAGTTTGGTTTTACCGTT+AGG - chr1_1:7466515-7466534 None:intergenic 35.0%
TCAGGGTAAACATAGTCATA+TGG - chr1_1:7466659-7466678 None:intergenic 35.0%
ATAAACGTGCCAATAACAAG+AGG - chr1_1:7466716-7466735 None:intergenic 35.0%
! TTTCAACGAGACTGATTTCT+TGG - chr1_1:7466744-7466763 None:intergenic 35.0%
! AAACCAACGGAGATGAATTT+TGG + chr1_1:7466762-7466781 Msa0005030:five_prime_UTR 35.0%
ATTGGTCGGACAATTATTGT+TGG + chr1_1:7466860-7466879 Msa0005030:five_prime_UTR 35.0%
ACCACCTAAATATGAACTTG+TGG + chr1_1:7466916-7466935 Msa0005030:five_prime_UTR 35.0%
ACCACAAGTTCATATTTAGG+TGG - chr1_1:7466920-7466939 None:intergenic 35.0%
TATGAACTTGTGGTTAATGG+TGG + chr1_1:7466926-7466945 Msa0005030:five_prime_UTR 35.0%
ATGAACTTGTGGTTAATGGT+GGG + chr1_1:7466927-7466946 Msa0005030:five_prime_UTR 35.0%
AAAATTCTTAGTGGAGAGTG+AGG + chr1_1:7466955-7466974 Msa0005030:five_prime_UTR 35.0%
AAATTCTTAGTGGAGAGTGA+GGG + chr1_1:7466956-7466975 Msa0005030:five_prime_UTR 35.0%
GTGGACATTTATGAGTGTTT+AGG + chr1_1:7466992-7467011 Msa0005030:five_prime_UTR 35.0%
! TGTGTTTAGGTTTGATGAGA+AGG + chr1_1:7467051-7467070 Msa0005030:five_prime_UTR 35.0%
!! ATGGGTTGATTTGATGAGTT+TGG + chr1_1:7467081-7467100 Msa0005030:five_prime_UTR 35.0%
!! TGGGTTGATTTGATGAGTTT+GGG + chr1_1:7467082-7467101 Msa0005030:five_prime_UTR 35.0%
ATTTGATGAGTTTGGGAGAT+AGG + chr1_1:7467089-7467108 Msa0005030:five_prime_UTR 35.0%
TTTGATGAGTTTGGGAGATA+GGG + chr1_1:7467090-7467109 Msa0005030:five_prime_UTR 35.0%
!!! GGAGATAGGGTTTTGTTTTT+GGG + chr1_1:7467103-7467122 Msa0005030:five_prime_UTR 35.0%
!!! GAGATAGGGTTTTGTTTTTG+GGG + chr1_1:7467104-7467123 Msa0005030:five_prime_UTR 35.0%
!! TGTATCTTTGACTTGGATCA+AGG + chr1_1:7467242-7467261 Msa0005030:CDS 35.0%
GCAAGATAACATACTCTGTT+AGG - chr1_1:7467350-7467369 None:intergenic 35.0%
AAATATATGCTCAAGGAGAC+TGG - chr1_1:7467660-7467679 None:intergenic 35.0%
TAGAGGATCATGTGATCAAA+TGG + chr1_1:7467840-7467859 Msa0005030:intron 35.0%
AAGATCCAAATATAGGGTTG+AGG + chr1_1:7468021-7468040 Msa0005030:intron 35.0%
GATTTCCTCAACCCTATATT+TGG - chr1_1:7468029-7468048 None:intergenic 35.0%
!!! CATTTTTAAGTTCGGGATCT+TGG - chr1_1:7468067-7468086 None:intergenic 35.0%
AGTTTAAGAAACTGGCCAAA+TGG + chr1_1:7468183-7468202 Msa0005030:intron 35.0%
TTTATCTGCATAGACTCTCA+AGG - chr1_1:7468275-7468294 None:intergenic 35.0%
TGAGAGTCTATGCAGATAAA+AGG + chr1_1:7468275-7468294 Msa0005030:intron 35.0%
GAAGTTTCTAACAATGATGC+TGG + chr1_1:7468345-7468364 Msa0005030:intron 35.0%
!! AATGCATAACTTGCTTCTCT+AGG + chr1_1:7468437-7468456 Msa0005030:intron 35.0%
! GTTTGTATATAAGTTGGGGA+TGG - chr1_1:7468468-7468487 None:intergenic 35.0%
TAATCAATCCTCTCTGTGTT+TGG + chr1_1:7468717-7468736 Msa0005030:intron 35.0%
AGACAACAGATGGAGATAAT+AGG - chr1_1:7468746-7468765 None:intergenic 35.0%
CTTAACTGAATGCAAACTCT+TGG + chr1_1:7468780-7468799 Msa0005030:intron 35.0%
AAACAGAGAAATAAAGAGGG+CGG - chr1_1:7469199-7469218 None:intergenic 35.0%
ACATTATGGTCATCACTAAG+TGG - chr1_1:7469252-7469271 None:intergenic 35.0%
ACTTAGTGATGACCATAATG+TGG + chr1_1:7469251-7469270 Msa0005030:intron 35.0%
! AACAGCAAAATGTTTCTGCA+AGG - chr1_1:7469279-7469298 None:intergenic 35.0%
!! TTGCAGAAACATTTTGCTGT+TGG + chr1_1:7469278-7469297 Msa0005030:intron 35.0%
!!! TGCAGAAACATTTTGCTGTT+GGG + chr1_1:7469279-7469298 Msa0005030:intron 35.0%
CTCTTGACTCAACATTTAAG+AGG - chr1_1:7469437-7469456 None:intergenic 35.0%
TGTTGAGTCAAGAGAGATAT+TGG + chr1_1:7469443-7469462 Msa0005030:intron 35.0%
AGAGAGATATTGGAGGAAAA+TGG + chr1_1:7469453-7469472 Msa0005030:intron 35.0%
GAGAGATATTGGAGGAAAAT+GGG + chr1_1:7469454-7469473 Msa0005030:intron 35.0%
AGAGATATTGGAGGAAAATG+GGG + chr1_1:7469455-7469474 Msa0005030:intron 35.0%
GGTAAGGAATATGCATTACA+AGG + chr1_1:7469476-7469495 Msa0005030:intron 35.0%
TCAGATGTAGAAGCTCCTTT+GGG - chr1_1:7466268-7466287 None:intergenic 40.0%
ATCAGATGTAGAAGCTCCTT+TGG - chr1_1:7466269-7466288 None:intergenic 40.0%
GGTTAGATTGGGAATTGGTA+AGG - chr1_1:7466363-7466382 None:intergenic 40.0%
TTGAGGGTTAGATTGGGAAT+TGG - chr1_1:7466368-7466387 None:intergenic 40.0%
GGAAACTTGAGGGTTAGATT+GGG - chr1_1:7466374-7466393 None:intergenic 40.0%
TGGAAACTTGAGGGTTAGAT+TGG - chr1_1:7466375-7466394 None:intergenic 40.0%
! TGTTGATGGGAGATTGATAG+GGG - chr1_1:7466400-7466419 None:intergenic 40.0%
! GTTGTTGATGGGAGATTGAT+AGG - chr1_1:7466402-7466421 None:intergenic 40.0%
ATGATTGATACGAGAGGAGT+GGG - chr1_1:7466541-7466560 None:intergenic 40.0%
GATGAGATGATTGATACGAG+AGG - chr1_1:7466547-7466566 None:intergenic 40.0%
! TGACAGCAACGATCTTTTCA+GGG - chr1_1:7466676-7466695 None:intergenic 40.0%
TCAGTCTCGTTGAAAACCAA+CGG + chr1_1:7466749-7466768 Msa0005030:five_prime_UTR 40.0%
ACACCAAAATTCATCTCCGT+TGG - chr1_1:7466768-7466787 None:intergenic 40.0%
! GAGACATCTGCGTTTTCAAA+GGG + chr1_1:7466816-7466835 Msa0005030:five_prime_UTR 40.0%
CGGACAATTATTGTTGGACT+TGG + chr1_1:7466866-7466885 Msa0005030:five_prime_UTR 40.0%
TGAACTTGTGGTTAATGGTG+GGG + chr1_1:7466928-7466947 Msa0005030:five_prime_UTR 40.0%
TGGGGGCAAAAAATTCTTAG+TGG + chr1_1:7466946-7466965 Msa0005030:five_prime_UTR 40.0%
TTGATGAGAAGGAGAGTGAA+TGG + chr1_1:7467062-7467081 Msa0005030:five_prime_UTR 40.0%
TGATGAGAAGGAGAGTGAAT+GGG + chr1_1:7467063-7467082 Msa0005030:five_prime_UTR 40.0%
!!! GGGAGATAGGGTTTTGTTTT+TGG + chr1_1:7467102-7467121 Msa0005030:five_prime_UTR 40.0%
!!! AGGGTTTTGTTTTTGGGGAA+TGG + chr1_1:7467109-7467128 Msa0005030:exon 40.0%
TGGGAACTGTATCTTTGACT+TGG + chr1_1:7467235-7467254 Msa0005030:CDS 40.0%
TTCACCTTTGTCTGATTGTC+CGG + chr1_1:7467271-7467290 Msa0005030:CDS 40.0%
TATTCCGGACAATCAGACAA+AGG - chr1_1:7467278-7467297 None:intergenic 40.0%
!!! TCCAGACCTTTGTTGTTTTC+TGG + chr1_1:7467868-7467887 Msa0005030:intron 40.0%
TCCAGAAAACAACAAAGGTC+TGG - chr1_1:7467872-7467891 None:intergenic 40.0%
TTACTGCTAAAAGGTCGACA+TGG - chr1_1:7468003-7468022 None:intergenic 40.0%
TAAGGGACACTGATACCATT+TGG - chr1_1:7468201-7468220 None:intergenic 40.0%
CAATTATCACATGACCATGG+TGG + chr1_1:7468541-7468560 Msa0005030:intron 40.0%
! ATTGTGACTTTTGACCACCA+TGG - chr1_1:7468558-7468577 None:intergenic 40.0%
TATCAGTATGTACTCTGCCA+TGG + chr1_1:7468602-7468621 Msa0005030:intron 40.0%
GATTATGTAACATGCCCTAG+AGG - chr1_1:7468702-7468721 None:intergenic 40.0%
ATAGGATTCCAAACACAGAG+AGG - chr1_1:7468728-7468747 None:intergenic 40.0%
AACAGAGAAATAAAGAGGGC+GGG - chr1_1:7469198-7469217 None:intergenic 40.0%
CACATGGTTTCATATGCTCA+AGG - chr1_1:7469338-7469357 None:intergenic 40.0%
CATATAGCCATAGCTAATCC+CGG + chr1_1:7469381-7469400 Msa0005030:intron 40.0%
AAAATACCTAGGAGACAGAC+CGG - chr1_1:7469403-7469422 None:intergenic 40.0%
TGAGTCAAGAGAGATATTGG+AGG + chr1_1:7469446-7469465 Msa0005030:intron 40.0%
TATTGGAGGAAAATGGGGTA+AGG + chr1_1:7469460-7469479 Msa0005030:intron 40.0%
TACAAGGAGTTCCATAATGC+TGG + chr1_1:7469492-7469511 Msa0005030:intron 40.0%
ACAAGGAGTTCCATAATGCT+GGG + chr1_1:7469493-7469512 Msa0005030:intron 40.0%
GTTCTGTCTACAAAACTCGA+GGG - chr1_1:7469557-7469576 None:intergenic 40.0%
TGTTCTGTCTACAAAACTCG+AGG - chr1_1:7469558-7469577 None:intergenic 40.0%
TCTGCCATTGATGTTGCTGT+TGG - chr1_1:7466227-7466246 None:intergenic 45.0%
! ATAGGGGTGTGGAAACTTGA+GGG - chr1_1:7466384-7466403 None:intergenic 45.0%
GGAGAGAGAGTGAAATTGAG+TGG - chr1_1:7466426-7466445 None:intergenic 45.0%
!! GATGAGGAAGAAAGAGCGTT+TGG - chr1_1:7466447-7466466 None:intergenic 45.0%
GTTGAAGTGGTGGTTTGATG+AGG - chr1_1:7466463-7466482 None:intergenic 45.0%
GATGATTGATACGAGAGGAG+TGG - chr1_1:7466542-7466561 None:intergenic 45.0%
! CACTAAAATCGAGAGCGTGA+GGG - chr1_1:7466580-7466599 None:intergenic 45.0%
! TCACTAAAATCGAGAGCGTG+AGG - chr1_1:7466581-7466600 None:intergenic 45.0%
! GTGACAGCAACGATCTTTTC+AGG - chr1_1:7466677-7466696 None:intergenic 45.0%
! GGAGACATCTGCGTTTTCAA+AGG + chr1_1:7466815-7466834 Msa0005030:five_prime_UTR 45.0%
! ACATCTGCGTTTTCAAAGGG+CGG + chr1_1:7466819-7466838 Msa0005030:five_prime_UTR 45.0%
GATCGCATGACTTCGATAAC+TGG + chr1_1:7466888-7466907 Msa0005030:five_prime_UTR 45.0%
GAACTTGTGGTTAATGGTGG+GGG + chr1_1:7466929-7466948 Msa0005030:five_prime_UTR 45.0%
CATTTGTCGATCCACTCTGT+AGG - chr1_1:7467320-7467339 None:intergenic 45.0%
TCAACGAATCCGTTGACACT+AGG + chr1_1:7467713-7467732 Msa0005030:intron 45.0%
AGACTGACACCTAGTGTCAA+CGG - chr1_1:7467725-7467744 None:intergenic 45.0%
GTTGGGATATTACGAGCCTT+CGG - chr1_1:7467796-7467815 None:intergenic 45.0%
! CATCGAGCTTTTGTATCTGC+CGG + chr1_1:7467900-7467919 Msa0005030:intron 45.0%
!! TTGTATCTGCCGGTGTCATT+TGG + chr1_1:7467910-7467929 Msa0005030:intron 45.0%
CCTTCAACACCAAATGACAC+CGG - chr1_1:7467922-7467941 None:intergenic 45.0%
GGTCGACATGGAGAAGAAAA+AGG - chr1_1:7467991-7468010 None:intergenic 45.0%
TATACACCGGACATCACCAT+TGG + chr1_1:7468308-7468327 Msa0005030:intron 45.0%
! AACTTGCCAATGGTGATGTC+CGG - chr1_1:7468317-7468336 None:intergenic 45.0%
AATAAGAGGCAGAGTGTGTC+AGG + chr1_1:7468514-7468533 Msa0005030:intron 45.0%
ATGCGCTTGAAGACAACAGA+TGG - chr1_1:7468756-7468775 None:intergenic 45.0%
AAGACATGGGGCACAACATT+GGG + chr1_1:7469135-7469154 Msa0005030:intron 45.0%
ACAGAGAAATAAAGAGGGCG+GGG - chr1_1:7469197-7469216 None:intergenic 45.0%
TTCTGCAAGGAGCCACATTA+TGG - chr1_1:7469266-7469285 None:intergenic 45.0%
! GAAACCATGTGCTTCGAGTT+AGG + chr1_1:7469347-7469366 Msa0005030:intron 45.0%
GAATCCTAACTCGAAGCACA+TGG - chr1_1:7469354-7469373 None:intergenic 45.0%
AAATACCTAGGAGACAGACC+GGG - chr1_1:7469402-7469421 None:intergenic 45.0%
CAAGGAGTTCCATAATGCTG+GGG + chr1_1:7469494-7469513 Msa0005030:intron 45.0%
CTTATATGGCCCCAGCATTA+TGG - chr1_1:7469506-7469525 None:intergenic 45.0%
ATAGCACTAACAGACCCAAG+AGG + chr1_1:7469789-7469808 Msa0005030:CDS 45.0%
CATCAATGGCAGAGTCAGAC+TGG + chr1_1:7466234-7466253 Msa0005030:five_prime_UTR 50.0%
!! AGACTGGTCTTCACTCCCAA+AGG + chr1_1:7466250-7466269 Msa0005030:five_prime_UTR 50.0%
GAGTTCAACGTCGAGTCGTT+GGG - chr1_1:7466294-7466313 None:intergenic 50.0%
! TCCGATCAACTTGTTCCGCA+TGG + chr1_1:7466327-7466346 Msa0005030:five_prime_UTR 50.0%
!! GCCATGCGGAACAAGTTGAT+CGG - chr1_1:7466331-7466350 None:intergenic 50.0%
!! TAAGGAAGAAGAGCGCCATG+CGG - chr1_1:7466345-7466364 None:intergenic 50.0%
! GATAGGGGTGTGGAAACTTG+AGG - chr1_1:7466385-7466404 None:intergenic 50.0%
ATGGGAGATTGATAGGGGTG+TGG - chr1_1:7466395-7466414 None:intergenic 50.0%
!! ATCAACCAAGGGCGTTGAAG+TGG - chr1_1:7466476-7466495 None:intergenic 50.0%
GAGGAGTGGGTGTAAGAGTT+TGG - chr1_1:7466528-7466547 None:intergenic 50.0%
AGAGCGTGAGGGAAAAGAAG+AGG - chr1_1:7466569-7466588 None:intergenic 50.0%
! GGGCGGCCTTATATGATTGA+TGG + chr1_1:7466836-7466855 Msa0005030:five_prime_UTR 50.0%
TTGTCGATCCACTCTGTAGG+TGG - chr1_1:7467317-7467336 None:intergenic 50.0%
!! CCGGTGTCATTTGGTGTTGA+AGG + chr1_1:7467919-7467938 Msa0005030:intron 50.0%
CAGTATGTACTCTGCCATGG+TGG + chr1_1:7468605-7468624 Msa0005030:intron 50.0%
TACTCTGCCATGGTGGTCAA+AGG + chr1_1:7468612-7468631 Msa0005030:intron 50.0%
GTTAGTGCCTTTGACCACCA+TGG - chr1_1:7468622-7468641 None:intergenic 50.0%
GAAGACATGGGGCACAACAT+TGG + chr1_1:7469134-7469153 Msa0005030:intron 50.0%
AGAGAAATAAAGAGGGCGGG+GGG - chr1_1:7469195-7469214 None:intergenic 50.0%
CAGAGAAATAAAGAGGGCGG+GGG - chr1_1:7469196-7469215 None:intergenic 50.0%
TGGTCATCACTAAGTGGCAG+AGG - chr1_1:7469246-7469265 None:intergenic 50.0%
!! AGACAGACCGGGATTAGCTA+TGG - chr1_1:7469391-7469410 None:intergenic 50.0%
CGAGTTCAACGTCGAGTCGT+TGG - chr1_1:7466295-7466314 None:intergenic 55.0%
AACCACCACTTCAACGCCCT+TGG + chr1_1:7466468-7466487 Msa0005030:five_prime_UTR 55.0%
!! AACCAAGGGCGTTGAAGTGG+TGG - chr1_1:7466473-7466492 None:intergenic 55.0%
TGTTCTGGCCACCTACAGAG+TGG + chr1_1:7467306-7467325 Msa0005030:CDS 55.0%
GAGAAATAAAGAGGGCGGGG+GGG - chr1_1:7469194-7469213 None:intergenic 55.0%
CTAATCCCGGTCTGTCTCCT+AGG + chr1_1:7469394-7469413 Msa0005030:intron 55.0%
! TGAGGGCGCGTTGCTGCGAG+TGG + chr1_1:7466973-7466992 Msa0005030:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 7466224 7469820 7466224 ID=Msa0005030;Name=Msa0005030
chr1_1 mRNA 7466224 7469820 7466224 ID=Msa0005030-mRNA-1;Parent=Msa0005030;Name=Msa0005030-mRNA-1;_AED=0.20;_eAED=0.26;_QI=904|0|0.33|1|0|0.33|3|0|104
chr1_1 exon 7466224 7467355 7466224 ID=Msa0005030-mRNA-1:exon:2618;Parent=Msa0005030-mRNA-1
chr1_1 exon 7469570 7469578 7469570 ID=Msa0005030-mRNA-1:exon:2619;Parent=Msa0005030-mRNA-1
chr1_1 exon 7469743 7469820 7469743 ID=Msa0005030-mRNA-1:exon:2620;Parent=Msa0005030-mRNA-1
chr1_1 five_prime_UTR 7466224 7467127 7466224 ID=Msa0005030-mRNA-1:five_prime_utr;Parent=Msa0005030-mRNA-1
chr1_1 CDS 7467128 7467355 7467128 ID=Msa0005030-mRNA-1:cds;Parent=Msa0005030-mRNA-1
chr1_1 CDS 7469570 7469578 7469570 ID=Msa0005030-mRNA-1:cds;Parent=Msa0005030-mRNA-1
chr1_1 CDS 7469743 7469820 7469743 ID=Msa0005030-mRNA-1:cds;Parent=Msa0005030-mRNA-1
Gene Sequence

>Msa0005030

ATGGATGTTCGTTTTCTGCTTCTGCTTCAGATTTGTGTTTCTAAAGGGAATTGTGTCATCTTTATTGATGTTGCATACTTCAATTTTAATTGTAAAAACATGCAATATGGGAACTGTATCTTTGACTTGGATCAAGGCAAGCTTTCACCTTTGTCTGATTGTCCGGAATATTTTAACTTGTTCTGGCCACCTACAGAGTGGATCGACAAATGCTGCATTCCTAACAGAACAGAACATAGCTTCTTTCATCATTTAGTAAAGATCTGTAATGATATTGGTGCAAATAGCACTAACAGACCCAAGAGGAAAATATAA
Protein sequence

>Msa0005030

MDVRFLLLLQICVSKGNCVIFIDVAYFNFNCKNMQYGNCIFDLDQGKLSPLSDCPEYFNLFWPPTEWIDKCCIPNRTEHSFFHHLVKICNDIGANSTNRPKRKI*