AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0017890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0017890 MtrunA17_Chr1g0152051 97.196 214 6 0 1 214 1 214 1.80e-155 429
Msa0017890 MtrunA17_Chr3g0135221 58.294 211 65 3 21 211 13 220 1.19e-69 212
Msa0017890 MtrunA17_Chr3g0135211 43.721 215 104 5 1 213 1 200 1.32e-48 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0017890 AT4G07990.2 57.944 214 79 6 5 210 4 214 4.29e-76 228
Msa0017890 AT4G07990.1 54.386 228 79 7 5 210 4 228 6.39e-72 218
Msa0017890 AT4G07990.3 55.385 195 76 6 5 191 4 195 1.05e-65 201

Find 69 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAGGAAAACCTTAATTT+TGG 0.106710 1_1:+29902405 Msa0017890:three_prime_UTR
GTAATAAATACCCAAAATTA+AGG 0.233384 1_1:-29902416 None:intergenic
AACACACCACTACACGGTTT+TGG 0.249771 1_1:+29900012 Msa0017890:CDS
CAGCAGCCTAATGTGTATTT+AGG 0.266150 1_1:+29902513 Msa0017890:three_prime_UTR
TTTATTTCAGCATCTGTATA+TGG 0.277581 1_1:-29901075 None:intergenic
TCGAATACCAATCATCTCTT+TGG 0.278893 1_1:-29899910 None:intergenic
ATTCTTTCTTTCTTGTTGTA+TGG 0.287118 1_1:-29902260 None:intergenic
TTCAATACATAGCATCCTTC+AGG 0.293400 1_1:-29902323 None:intergenic
CGAGACACCCCAAGGGAAAA+TGG 0.304097 1_1:+29899971 Msa0017890:CDS
AACATAGAGATAGTGGTTAT+TGG 0.305304 1_1:+29898387 Msa0017890:five_prime_UTR
TCTCTTTATAGGAAAGAATA+AGG 0.308036 1_1:+29899789 Msa0017890:intron
CTCTTTCGCAGACAGCATTT+AGG 0.317338 1_1:+29902074 Msa0017890:intron
TTCAGGGCCCATGGATTATC+AGG 0.323315 1_1:-29902306 None:intergenic
TTAGAAGAAAATACGAGAAT+TGG 0.328205 1_1:+29899852 Msa0017890:CDS
AGTTCAAGAATTCAAGCTTT+GGG 0.342435 1_1:+29898517 Msa0017890:CDS
TACGAGAATTGGAAGGAAAA+TGG 0.359437 1_1:+29899863 Msa0017890:CDS
TTATCCTCTTGGTTCTGATC+AGG 0.372747 1_1:-29902118 None:intergenic
AAAAGAACCGCCTTTCGATC+TGG 0.374122 1_1:-29899034 None:intergenic
ATATACAGATGCTGAAATAA+AGG 0.398272 1_1:+29901077 Msa0017890:CDS
GAATTTGATATAACTGAGAT+TGG 0.404689 1_1:+29898432 Msa0017890:five_prime_UTR
ACCAATCATCTCTTTGGAAA+TGG 0.406526 1_1:-29899904 None:intergenic
GGGTATTTATTACACAATAG+TGG 0.419416 1_1:+29902426 Msa0017890:three_prime_UTR
AATTGGAAGGAAAATGGTTC+TGG 0.420102 1_1:+29899869 Msa0017890:CDS
AAGACCTACCTTTATCCTCT+TGG 0.428497 1_1:-29902129 None:intergenic
AAGTTCAAGAATTCAAGCTT+TGG 0.435666 1_1:+29898516 Msa0017890:CDS
AAAGTGTGTTTAATAGAGAA+AGG 0.440344 1_1:+29899819 Msa0017890:CDS
ACCATTTCCAAAGAGATGAT+TGG 0.453905 1_1:+29899903 Msa0017890:CDS
GATCAGAACCAAGAGGATAA+AGG 0.457480 1_1:+29902121 Msa0017890:CDS
AATTGAGAACATAGAGATAG+TGG 0.471746 1_1:+29898380 Msa0017890:five_prime_UTR
AATTGCGCCGATTATTCAAA+AGG 0.474087 1_1:+29898345 Msa0017890:five_prime_UTR
CACAATGGTGTTGTTATCAT+AGG 0.475563 1_1:+29902457 Msa0017890:three_prime_UTR
TCAATACATAGCATCCTTCA+GGG 0.485739 1_1:-29902322 None:intergenic
CGGGAATCTCATGCAGCAAA+GGG 0.486692 1_1:+29898310 Msa0017890:five_prime_UTR
GCAAGAGGAGTACGAGGAAG+AGG 0.489481 1_1:+29899104 Msa0017890:CDS
TTCATACCTAAATACACATT+AGG 0.493459 1_1:-29902519 None:intergenic
CAACCTTGCCTGATAATCCA+TGG 0.498944 1_1:+29902298 Msa0017890:three_prime_UTR
AAGTGTGTTTAATAGAGAAA+GGG 0.502349 1_1:+29899820 Msa0017890:CDS
AGCTAAGTTCAAAGAAGTAA+TGG 0.518736 1_1:+29902225 Msa0017890:CDS
AGAACTCACGGCGAAGGTGG+TGG 0.531498 1_1:-29898573 None:intergenic
TTCAAGAAGAAGCTTGGAAG+CGG 0.541521 1_1:+29899067 Msa0017890:CDS
ACGGGAATCTCATGCAGCAA+AGG 0.545446 1_1:+29898309 None:intergenic
TTGTAGTTGTCCAGATCGAA+AGG 0.551804 1_1:+29899024 Msa0017890:intron
GTTCAAGAATTCAAGCTTTG+GGG 0.563262 1_1:+29898518 Msa0017890:CDS
ATAGCATCCTTCAGGGCCCA+TGG 0.566617 1_1:-29902315 None:intergenic
CGAGGAAGAGGCTGAGAGAG+TGG 0.567175 1_1:+29899116 Msa0017890:CDS
GTATTTAGGTATGAAACAAA+TGG 0.570641 1_1:+29902527 Msa0017890:three_prime_UTR
CTAAGGTTGATGTAGTCCAC+TGG 0.583757 1_1:+29902372 Msa0017890:three_prime_UTR
TGATAATCCATGGGCCCTGA+AGG 0.584636 1_1:+29902308 Msa0017890:three_prime_UTR
AACAAACCTTTAGAGACCAA+TGG 0.589429 1_1:+29899939 Msa0017890:CDS
ACTCACGGCGAAGGTGGTGG+CGG 0.589497 1_1:-29898570 None:intergenic
AACCTTGCCTGATAATCCAT+GGG 0.589677 1_1:+29902299 Msa0017890:three_prime_UTR
AGGCGTAGAACTCACGGCGA+AGG 0.592392 1_1:-29898579 None:intergenic
TTCATTAACACACCACTACA+CGG 0.593016 1_1:+29900006 Msa0017890:CDS
AAGAAAATACGAGAATTGGA+AGG 0.602692 1_1:+29899856 Msa0017890:CDS
GCAGACAGCATTTAGGACGA+AGG 0.616195 1_1:+29902081 Msa0017890:intron
TAAATGAAGGAAGATGAAGA+AGG 0.617251 1_1:+29898467 Msa0017890:exon
GGCCCATGGATTATCAGGCA+AGG 0.631445 1_1:-29902301 None:intergenic
CGTAGAACTCACGGCGAAGG+TGG 0.635643 1_1:-29898576 None:intergenic
GTACAATAAGCGATTGCAAG+AGG 0.639039 1_1:+29899089 Msa0017890:CDS
CAAGACCCAAAACCGTGTAG+TGG 0.644029 1_1:-29900018 None:intergenic
AAAATCACCGAGACACCCCA+AGG 0.648601 1_1:+29899963 Msa0017890:CDS
GCGATTGCAAGAGGAGTACG+AGG 0.657325 1_1:+29899098 Msa0017890:CDS
AAATCACCGAGACACCCCAA+GGG 0.664599 1_1:+29899964 Msa0017890:CDS
TAGCACCAATTAAACCGAAG+AGG 0.665737 1_1:-29898547 None:intergenic
CACTGGTATGCTGAAAACCA+AGG 0.671896 1_1:+29902389 Msa0017890:three_prime_UTR
TCATCCTGATCAGAACCAAG+AGG 0.690141 1_1:+29902114 Msa0017890:CDS
TAGTTGTCCAGATCGAAAGG+CGG 0.694057 1_1:+29899027 Msa0017890:intron
AGAAGTAATGGATTCATACG+AGG 0.703337 1_1:+29902237 Msa0017890:CDS
ATAGTGGTGTTGTATCACAA+TGG 0.785001 1_1:+29902442 Msa0017890:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTTTTAAAAAAA+GGG - chr1_1:29900888-29900907 None:intergenic 0.0%
!!! TTATTTGATTATTTTTGAAA+TGG + chr1_1:29902569-29902588 Msa0017890:three_prime_UTR 10.0%
!!! ATTTTATTTTACGTCATTTT+AGG + chr1_1:29898623-29898642 Msa0017890:intron 15.0%
!!! AGTTCTTATTTACATTTTTT+TGG + chr1_1:29898929-29898948 Msa0017890:intron 15.0%
!!! CTAAATATAAAAGCATAATA+TGG + chr1_1:29900380-29900399 Msa0017890:intron 15.0%
!!! GTTGATTTTTTTCTAAATGA+AGG + chr1_1:29898454-29898473 Msa0017890:exon 20.0%
!! TATATGTGACTAATCATAAA+TGG - chr1_1:29899705-29899724 None:intergenic 20.0%
!! ATATCAGAATGCTAAAATAT+AGG + chr1_1:29900099-29900118 Msa0017890:intron 20.0%
!!! ATAACAATAACTTTTCTAAG+TGG + chr1_1:29900621-29900640 Msa0017890:intron 20.0%
!!! ATAACAATAACTTTTCTAAG+TGG + chr1_1:29900621-29900640 Msa0017890:intron 20.0%
!! TATAATTTACAAACATCTTG+TGG - chr1_1:29901384-29901403 None:intergenic 20.0%
!!! ATTATACCAACTATTTTGAT+TGG + chr1_1:29901398-29901417 Msa0017890:intron 20.0%
!! AACATACTTATTATGATTAG+TGG + chr1_1:29901802-29901821 Msa0017890:intron 20.0%
!! ACATACTTATTATGATTAGT+GGG + chr1_1:29901803-29901822 Msa0017890:intron 20.0%
!! CATTATTACATAATGATTCA+TGG + chr1_1:29902175-29902194 Msa0017890:intron 20.0%
!!! GAAAATAAGCATAATTTTTG+AGG + chr1_1:29902343-29902362 Msa0017890:three_prime_UTR 20.0%
!! GTAATAAATACCCAAAATTA+AGG - chr1_1:29902419-29902438 None:intergenic 20.0%
! GAATTTGATATAACTGAGAT+TGG + chr1_1:29898432-29898451 Msa0017890:five_prime_UTR 25.0%
! GAAAATGAAAAGAACAAAGA+AGG - chr1_1:29898602-29898621 None:intergenic 25.0%
!!! ATAAAAGGTTTTGATGATAC+TGG - chr1_1:29898670-29898689 None:intergenic 25.0%
! ACTCTATATTCTGTTAACAT+GGG + chr1_1:29898900-29898919 Msa0017890:intron 25.0%
!!! ACCTGATTTTATTGTTATTG+TGG + chr1_1:29899183-29899202 Msa0017890:intron 25.0%
! ACCACAATAACAATAAAATC+AGG - chr1_1:29899187-29899206 None:intergenic 25.0%
!!! GATGTTACGTTATTTTTAGA+GGG - chr1_1:29899250-29899269 None:intergenic 25.0%
!! TATCATGTGATCTTTGAAAA+TGG + chr1_1:29899275-29899294 Msa0017890:intron 25.0%
!! ATCATGTGATCTTTGAAAAT+GGG + chr1_1:29899276-29899295 Msa0017890:intron 25.0%
!! AAAATTTGCTTTGTCACAAA+AGG - chr1_1:29899308-29899327 None:intergenic 25.0%
!! AATCTCTATTAGTTGATTCT+AGG + chr1_1:29899333-29899352 Msa0017890:intron 25.0%
!! TATTAGTTGATTCTAGGAAT+TGG + chr1_1:29899339-29899358 Msa0017890:intron 25.0%
!! TGTTTCTATCTTCTCTTTAT+AGG + chr1_1:29899778-29899797 Msa0017890:intron 25.0%
! TCTCTTTATAGGAAAGAATA+AGG + chr1_1:29899789-29899808 Msa0017890:intron 25.0%
! AAAGTGTGTTTAATAGAGAA+AGG + chr1_1:29899819-29899838 Msa0017890:CDS 25.0%
! AAGTGTGTTTAATAGAGAAA+GGG + chr1_1:29899820-29899839 Msa0017890:CDS 25.0%
! TTAGAAGAAAATACGAGAAT+TGG + chr1_1:29899852-29899871 Msa0017890:CDS 25.0%
! TTCTAAGTGGATATATTGTA+TGG + chr1_1:29900634-29900653 Msa0017890:intron 25.0%
! TTCTAAGTGGATATATTGTA+TGG + chr1_1:29900634-29900653 Msa0017890:intron 25.0%
!! TTTGAAGCAGTATATGATTA+TGG + chr1_1:29900791-29900810 Msa0017890:intron 25.0%
! AAGGTATATGATTAAAGCAA+GGG - chr1_1:29900962-29900981 None:intergenic 25.0%
! TTTATTTCAGCATCTGTATA+TGG - chr1_1:29901078-29901097 None:intergenic 25.0%
! ATATACAGATGCTGAAATAA+AGG + chr1_1:29901077-29901096 Msa0017890:CDS 25.0%
!! CTCTTTTTAACACAATATCT+TGG + chr1_1:29901112-29901131 Msa0017890:intron 25.0%
! CATATGATAAATATGACAGA+GGG - chr1_1:29901195-29901214 None:intergenic 25.0%
!!! TTTTCGTTGATGTTATTTGA+AGG + chr1_1:29901229-29901248 Msa0017890:intron 25.0%
!!! TGAAATGCTCTAAAGTATAA+AGG + chr1_1:29901460-29901479 Msa0017890:intron 25.0%
! TCAAATATATTCAGCCTATA+CGG + chr1_1:29901494-29901513 Msa0017890:intron 25.0%
! AACTCAGAAATGTAAATATG+TGG + chr1_1:29901563-29901582 Msa0017890:intron 25.0%
! ACTCAGAAATGTAAATATGT+GGG + chr1_1:29901564-29901583 Msa0017890:intron 25.0%
!!! TTTGACCGGTTTTATATAAA+TGG - chr1_1:29901685-29901704 None:intergenic 25.0%
! ATTTATATAAAACCGGTCAA+AGG + chr1_1:29901684-29901703 Msa0017890:intron 25.0%
! TTTATATAAAACCGGTCAAA+GGG + chr1_1:29901685-29901704 Msa0017890:intron 25.0%
! TACAGAAAATCAAGGTAAAA+AGG - chr1_1:29901927-29901946 None:intergenic 25.0%
! GTAAGTATTACAGAAAATCA+AGG - chr1_1:29901935-29901954 None:intergenic 25.0%
!! TTTGACCTTTCTTTCATTTT+CGG + chr1_1:29902014-29902033 Msa0017890:intron 25.0%
! TATTACCGAAAATGAAAGAA+AGG - chr1_1:29902022-29902041 None:intergenic 25.0%
!! ATTCTTTCTTTCTTGTTGTA+TGG - chr1_1:29902263-29902282 None:intergenic 25.0%
! TTCATACCTAAATACACATT+AGG - chr1_1:29902522-29902541 None:intergenic 25.0%
! GTATTTAGGTATGAAACAAA+TGG + chr1_1:29902527-29902546 Msa0017890:three_prime_UTR 25.0%
! GCTTTCTCCTTTTGAATAAT+CGG - chr1_1:29898355-29898374 None:intergenic 30.0%
AATTGAGAACATAGAGATAG+TGG + chr1_1:29898380-29898399 Msa0017890:five_prime_UTR 30.0%
! AACATAGAGATAGTGGTTAT+TGG + chr1_1:29898387-29898406 Msa0017890:five_prime_UTR 30.0%
TAAATGAAGGAAGATGAAGA+AGG + chr1_1:29898467-29898486 Msa0017890:exon 30.0%
AAGTTCAAGAATTCAAGCTT+TGG + chr1_1:29898516-29898535 Msa0017890:CDS 30.0%
AGTTCAAGAATTCAAGCTTT+GGG + chr1_1:29898517-29898536 Msa0017890:CDS 30.0%
! ATTTTCCTCTTCGGTTTAAT+TGG + chr1_1:29898542-29898561 Msa0017890:CDS 30.0%
! TTTTCATAAGTTCTGCCAAA+TGG + chr1_1:29898825-29898844 Msa0017890:intron 30.0%
AACAGAATATAGAGTCTCTT+AGG - chr1_1:29898895-29898914 None:intergenic 30.0%
GACTCTATATTCTGTTAACA+TGG + chr1_1:29898899-29898918 Msa0017890:intron 30.0%
!!! CGATGTTACGTTATTTTTAG+AGG - chr1_1:29899251-29899270 None:intergenic 30.0%
ACATTCCGAACTTAAATCAA+CGG + chr1_1:29899456-29899475 Msa0017890:intron 30.0%
TAAGTGAAGTGACTATCATA+AGG - chr1_1:29899628-29899647 None:intergenic 30.0%
AAGAAAATACGAGAATTGGA+AGG + chr1_1:29899856-29899875 Msa0017890:CDS 30.0%
!! ATTTTTCCATTGGTCTCTAA+AGG - chr1_1:29899948-29899967 None:intergenic 30.0%
TTTAAACAAGTGCAACAACA+TGG - chr1_1:29900215-29900234 None:intergenic 30.0%
TCAGATCATTGTAGCAATTA+AGG - chr1_1:29900269-29900288 None:intergenic 30.0%
ACCTGAAAATGTGTTATAAG+TGG + chr1_1:29900311-29900330 Msa0017890:intron 30.0%
CCTGAAAATGTGTTATAAGT+GGG + chr1_1:29900312-29900331 Msa0017890:intron 30.0%
TACATAACTGGATTGTTCTA+GGG - chr1_1:29900347-29900366 None:intergenic 30.0%
TTACATAACTGGATTGTTCT+AGG - chr1_1:29900348-29900367 None:intergenic 30.0%
CTAACTCATTCTTACATAAC+TGG - chr1_1:29900359-29900378 None:intergenic 30.0%
CAGTTATGTAAGAATGAGTT+AGG + chr1_1:29900357-29900376 Msa0017890:intron 30.0%
AAATATGCTTATAAAGTGGC+GGG - chr1_1:29900504-29900523 None:intergenic 30.0%
CAAATATGCTTATAAAGTGG+CGG - chr1_1:29900505-29900524 None:intergenic 30.0%
CTGCAAATATGCTTATAAAG+TGG - chr1_1:29900508-29900527 None:intergenic 30.0%
ATATTGTATGGTACATCTAG+AGG + chr1_1:29900646-29900665 Msa0017890:intron 30.0%
TATTGTATGGTACATCTAGA+GGG + chr1_1:29900647-29900666 Msa0017890:intron 30.0%
!! TTTTTAAAAAAAGGGCAACC+CGG - chr1_1:29900880-29900899 None:intergenic 30.0%
AGGTATATGATTAAAGCAAG+GGG - chr1_1:29900961-29900980 None:intergenic 30.0%
! GAAGGTATATGATTAAAGCA+AGG - chr1_1:29900963-29900982 None:intergenic 30.0%
ACAATATCTTGGCATATTAC+TGG + chr1_1:29901123-29901142 Msa0017890:intron 30.0%
CCATATGATAAATATGACAG+AGG - chr1_1:29901196-29901215 None:intergenic 30.0%
CCTCTGTCATATTTATCATA+TGG + chr1_1:29901193-29901212 Msa0017890:intron 30.0%
TTAACTAGTGTCCAACAAAA+CGG - chr1_1:29901306-29901325 None:intergenic 30.0%
!! TATACTTTAGAGCATTTCAC+AGG - chr1_1:29901459-29901478 None:intergenic 30.0%
! CTCTGCCATTTATATAAAAC+CGG + chr1_1:29901677-29901696 Msa0017890:intron 30.0%
AGCTAAGTTCAAAGAAGTAA+TGG + chr1_1:29902225-29902244 Msa0017890:CDS 30.0%
!!! AAGCATAATTTTTGAGGTTG+TGG + chr1_1:29902349-29902368 Msa0017890:three_prime_UTR 30.0%
! ACCAAGGAAAACCTTAATTT+TGG + chr1_1:29902405-29902424 Msa0017890:three_prime_UTR 30.0%
! CCAAGGAAAACCTTAATTTT+GGG + chr1_1:29902406-29902425 Msa0017890:three_prime_UTR 30.0%
GGGTATTTATTACACAATAG+TGG + chr1_1:29902426-29902445 Msa0017890:three_prime_UTR 30.0%
!! AATTGCGCCGATTATTCAAA+AGG + chr1_1:29898345-29898364 Msa0017890:five_prime_UTR 35.0%
GTTCAAGAATTCAAGCTTTG+GGG + chr1_1:29898518-29898537 Msa0017890:CDS 35.0%
CGCTTATCGTTCACTATAAA+AGG - chr1_1:29898685-29898704 None:intergenic 35.0%
TAAACACTTGTGAGACCATT+TGG - chr1_1:29898843-29898862 None:intergenic 35.0%
! ATGTGATCTTTGAAAATGGG+AGG + chr1_1:29899279-29899298 Msa0017890:intron 35.0%
AGACGTATGATATAGATCGA+CGG + chr1_1:29899392-29899411 Msa0017890:intron 35.0%
!! CTCAACCGTTGATTTAAGTT+CGG - chr1_1:29899464-29899483 None:intergenic 35.0%
ATGAACTACCACATGAAACT+AGG + chr1_1:29899484-29899503 Msa0017890:intron 35.0%
TGAAATCACCTAGTTTCATG+TGG - chr1_1:29899495-29899514 None:intergenic 35.0%
TGCTTAGATGTTTCATTGCT+TGG + chr1_1:29899575-29899594 Msa0017890:intron 35.0%
TCATAAGTGACTATTACACG+TGG - chr1_1:29899664-29899683 None:intergenic 35.0%
TCAGAAGTGACTATCATTTG+TGG - chr1_1:29899742-29899761 None:intergenic 35.0%
TCACTTCTGATAAGTCGTTT+AGG + chr1_1:29899752-29899771 Msa0017890:intron 35.0%
TACGAGAATTGGAAGGAAAA+TGG + chr1_1:29899863-29899882 Msa0017890:CDS 35.0%
AATTGGAAGGAAAATGGTTC+TGG + chr1_1:29899869-29899888 Msa0017890:CDS 35.0%
ACCATTTCCAAAGAGATGAT+TGG + chr1_1:29899903-29899922 Msa0017890:CDS 35.0%
! ACCAATCATCTCTTTGGAAA+TGG - chr1_1:29899907-29899926 None:intergenic 35.0%
TCGAATACCAATCATCTCTT+TGG - chr1_1:29899913-29899932 None:intergenic 35.0%
! AACAAACCTTTAGAGACCAA+TGG + chr1_1:29899939-29899958 Msa0017890:CDS 35.0%
! TTATGCTTCCATTTTCCCTT+GGG - chr1_1:29899982-29900001 None:intergenic 35.0%
! TTTATGCTTCCATTTTCCCT+TGG - chr1_1:29899983-29900002 None:intergenic 35.0%
TTCATTAACACACCACTACA+CGG + chr1_1:29900006-29900025 Msa0017890:CDS 35.0%
! CCCACTTATAACACATTTTC+AGG - chr1_1:29900315-29900334 None:intergenic 35.0%
ATGGCCCAATAAAAACCAAA+TGG + chr1_1:29900810-29900829 Msa0017890:intron 35.0%
!!! CCCACCATTTGGTTTTTATT+GGG - chr1_1:29900817-29900836 None:intergenic 35.0%
CCCAATAAAAACCAAATGGT+GGG + chr1_1:29900814-29900833 Msa0017890:intron 35.0%
!!! TCCCACCATTTGGTTTTTAT+TGG - chr1_1:29900818-29900837 None:intergenic 35.0%
! TTGCTAACTTTTGATGCAGA+AGG - chr1_1:29900981-29901000 None:intergenic 35.0%
!! TCCTTCTTTTGATTGATCTC+AGG + chr1_1:29901040-29901059 Msa0017890:intron 35.0%
ACCTGAGATCAATCAAAAGA+AGG - chr1_1:29901044-29901063 None:intergenic 35.0%
AAATATGACAGAGGGTGATA+GGG - chr1_1:29901187-29901206 None:intergenic 35.0%
TAAATATGACAGAGGGTGAT+AGG - chr1_1:29901188-29901207 None:intergenic 35.0%
! CGTTGATGTTATTTGAAGGA+TGG + chr1_1:29901233-29901252 Msa0017890:intron 35.0%
!!! CTAGTTTTGTACCGTTTTGT+TGG + chr1_1:29901292-29901311 Msa0017890:intron 35.0%
CGAATGCCAATCAAAATAGT+TGG - chr1_1:29901407-29901426 None:intergenic 35.0%
TTCGACAAAATCCTATGGTA+TGG + chr1_1:29901423-29901442 Msa0017890:intron 35.0%
! AGAGCATTTCACAGGTAATT+TGG - chr1_1:29901451-29901470 None:intergenic 35.0%
ATATATTCAGCCTATACGGA+TGG + chr1_1:29901498-29901517 Msa0017890:intron 35.0%
TACATTTCTGAGTTACGTCA+CGG - chr1_1:29901557-29901576 None:intergenic 35.0%
GAAGAGTGAAAAAAATACGC+AGG - chr1_1:29901591-29901610 None:intergenic 35.0%
GAGAGAAAGAGAAGAGAAAT+GGG - chr1_1:29901647-29901666 None:intergenic 35.0%
AGAGAGAAAGAGAAGAGAAA+TGG - chr1_1:29901648-29901667 None:intergenic 35.0%
AATATCTTGTACCCTTTGAC+CGG - chr1_1:29901699-29901718 None:intergenic 35.0%
AGGGTACAAGATATTGATGA+TGG + chr1_1:29901704-29901723 Msa0017890:intron 35.0%
! GGGTACAAGATATTGATGAT+GGG + chr1_1:29901705-29901724 Msa0017890:intron 35.0%
!! GGTACAAGATATTGATGATG+GGG + chr1_1:29901706-29901725 Msa0017890:intron 35.0%
!! AGAAGTAATGGATTCATACG+AGG + chr1_1:29902237-29902256 Msa0017890:CDS 35.0%
TCAATACATAGCATCCTTCA+GGG - chr1_1:29902325-29902344 None:intergenic 35.0%
TTCAATACATAGCATCCTTC+AGG - chr1_1:29902326-29902345 None:intergenic 35.0%
!!! AATTTTTGAGGTTGTGGCTA+AGG + chr1_1:29902355-29902374 Msa0017890:three_prime_UTR 35.0%
! CCCAAAATTAAGGTTTTCCT+TGG - chr1_1:29902409-29902428 None:intergenic 35.0%
!! ATAGTGGTGTTGTATCACAA+TGG + chr1_1:29902442-29902461 Msa0017890:three_prime_UTR 35.0%
!! CACAATGGTGTTGTTATCAT+AGG + chr1_1:29902457-29902476 Msa0017890:three_prime_UTR 35.0%
!! CTTTGGGGAATTTTCCTCTT+CGG + chr1_1:29898533-29898552 Msa0017890:CDS 40.0%
TAGCACCAATTAAACCGAAG+AGG - chr1_1:29898550-29898569 None:intergenic 40.0%
TTGTAGTTGTCCAGATCGAA+AGG + chr1_1:29899024-29899043 Msa0017890:intron 40.0%
!! CAGCTTTTCAAGAAGAAGCT+TGG + chr1_1:29899061-29899080 Msa0017890:CDS 40.0%
TTCAAGAAGAAGCTTGGAAG+CGG + chr1_1:29899067-29899086 Msa0017890:CDS 40.0%
GTACAATAAGCGATTGCAAG+AGG + chr1_1:29899089-29899108 Msa0017890:CDS 40.0%
TGTGACACACAGTTACAATG+GGG - chr1_1:29899368-29899387 None:intergenic 40.0%
CTGTGACACACAGTTACAAT+GGG - chr1_1:29899369-29899388 None:intergenic 40.0%
ACTAGGTGATTTCAAACTGC+AGG + chr1_1:29899501-29899520 Msa0017890:intron 40.0%
TTCATTGCTTGGTTAGTGCT+AGG + chr1_1:29899586-29899605 Msa0017890:intron 40.0%
! TGTCTCGGTGATTTTTCCAT+TGG - chr1_1:29899958-29899977 None:intergenic 40.0%
! TATGCTTCCATTTTCCCTTG+GGG - chr1_1:29899981-29900000 None:intergenic 40.0%
AGAGTCTATTCAAGATCCCT+CGG + chr1_1:29900407-29900426 Msa0017890:intron 40.0%
TGATATAATGTCTGTCCCGA+GGG - chr1_1:29900426-29900445 None:intergenic 40.0%
AGACATTATATCAGGCACTC+AGG + chr1_1:29900433-29900452 Msa0017890:intron 40.0%
ATTATATCAGGCACTCAGGT+TGG + chr1_1:29900437-29900456 Msa0017890:intron 40.0%
TTATATCAGGCACTCAGGTT+GGG + chr1_1:29900438-29900457 Msa0017890:intron 40.0%
!! CAAACTGACTTGTAAGGCAA+GGG + chr1_1:29900475-29900494 Msa0017890:intron 40.0%
GACCATATCTCACTCAATGT+AGG + chr1_1:29900527-29900546 Msa0017890:intron 40.0%
AGCCTACATTGAGTGAGATA+TGG - chr1_1:29900532-29900551 None:intergenic 40.0%
GCCCAATAAAAACCAAATGG+TGG + chr1_1:29900813-29900832 Msa0017890:intron 40.0%
AATATGACAGAGGGTGATAG+GGG - chr1_1:29901186-29901205 None:intergenic 40.0%
TGGCATTCGACAAAATCCTA+TGG + chr1_1:29901418-29901437 Msa0017890:intron 40.0%
GTAATTTGGCACCATACCAT+AGG - chr1_1:29901437-29901456 None:intergenic 40.0%
TTCTCACAATCCATCCGTAT+AGG - chr1_1:29901511-29901530 None:intergenic 40.0%
CAATAAATAGTCCGTAGGCT+TGG + chr1_1:29901729-29901748 Msa0017890:intron 40.0%
!! TTATCCTCTTGGTTCTGATC+AGG - chr1_1:29902121-29902140 None:intergenic 40.0%
GATCAGAACCAAGAGGATAA+AGG + chr1_1:29902121-29902140 Msa0017890:CDS 40.0%
AGAACCAAGAGGATAAAGGT+AGG + chr1_1:29902125-29902144 Msa0017890:intron 40.0%
AAGACCTACCTTTATCCTCT+TGG - chr1_1:29902132-29902151 None:intergenic 40.0%
TGGATGTGAAAATGTGACAG+AGG + chr1_1:29902195-29902214 Msa0017890:intron 40.0%
AACCTTGCCTGATAATCCAT+GGG + chr1_1:29902299-29902318 Msa0017890:three_prime_UTR 40.0%
!!! GGAGCCATTGATTTTTGTGT+CGG + chr1_1:29902478-29902497 Msa0017890:three_prime_UTR 40.0%
!!! GAGCCATTGATTTTTGTGTC+GGG + chr1_1:29902479-29902498 Msa0017890:three_prime_UTR 40.0%
GTTCCCGACACAAAAATCAA+TGG - chr1_1:29902485-29902504 None:intergenic 40.0%
CAGCAGCCTAATGTGTATTT+AGG + chr1_1:29902513-29902532 Msa0017890:three_prime_UTR 40.0%
! CAAAGAAGGCGTAGAACTCA+CGG - chr1_1:29898588-29898607 None:intergenic 45.0%
TAGTTGTCCAGATCGAAAGG+CGG + chr1_1:29899027-29899046 Msa0017890:intron 45.0%
AAAAGAACCGCCTTTCGATC+TGG - chr1_1:29899037-29899056 None:intergenic 45.0%
GCTGTGACACACAGTTACAA+TGG - chr1_1:29899370-29899389 None:intergenic 45.0%
TGCAGTAAGCTGCAAAGAAG+TGG + chr1_1:29899542-29899561 Msa0017890:intron 45.0%
! AACACACCACTACACGGTTT+TGG + chr1_1:29900012-29900031 Msa0017890:CDS 45.0%
! ACACACCACTACACGGTTTT+GGG + chr1_1:29900013-29900032 Msa0017890:CDS 45.0%
GAGTCTATTCAAGATCCCTC+GGG + chr1_1:29900408-29900427 Msa0017890:intron 45.0%
CTGATATAATGTCTGTCCCG+AGG - chr1_1:29900427-29900446 None:intergenic 45.0%
CTCGGGACAGACATTATATC+AGG + chr1_1:29900425-29900444 Msa0017890:intron 45.0%
! TATATCAGGCACTCAGGTTG+GGG + chr1_1:29900439-29900458 Msa0017890:intron 45.0%
CCTTGCCTTACAAGTCAGTT+TGG - chr1_1:29900477-29900496 None:intergenic 45.0%
!! CCAAACTGACTTGTAAGGCA+AGG + chr1_1:29900474-29900493 Msa0017890:intron 45.0%
TGGGGCAATAAATAGTCCGT+AGG + chr1_1:29901724-29901743 Msa0017890:intron 45.0%
CCACAAAACTTCCAAGCCTA+CGG - chr1_1:29901743-29901762 None:intergenic 45.0%
CTCTTTCGCAGACAGCATTT+AGG + chr1_1:29902074-29902093 Msa0017890:intron 45.0%
TCATCCTGATCAGAACCAAG+AGG + chr1_1:29902114-29902133 Msa0017890:CDS 45.0%
CAACCTTGCCTGATAATCCA+TGG + chr1_1:29902298-29902317 Msa0017890:three_prime_UTR 45.0%
!! CTAAGGTTGATGTAGTCCAC+TGG + chr1_1:29902372-29902391 Msa0017890:three_prime_UTR 45.0%
!! CTTGGTTTTCAGCATACCAG+TGG - chr1_1:29902391-29902410 None:intergenic 45.0%
CACTGGTATGCTGAAAACCA+AGG + chr1_1:29902389-29902408 Msa0017890:three_prime_UTR 45.0%
!!! TAAATTTTTTTATTCTTTAT+AGG - chr1_1:29900179-29900198 None:intergenic 5.0%
!!! CATTTTTTTTTTTTAAAAAA+AGG - chr1_1:29900889-29900908 None:intergenic 5.0%
!!! TTTTATTTAAGAAATAATAA+TGG - chr1_1:29901270-29901289 None:intergenic 5.0%
CGGGAATCTCATGCAGCAAA+GGG + chr1_1:29898310-29898329 Msa0017890:five_prime_UTR 50.0%
AAAATCACCGAGACACCCCA+AGG + chr1_1:29899963-29899982 Msa0017890:CDS 50.0%
AAATCACCGAGACACCCCAA+GGG + chr1_1:29899964-29899983 Msa0017890:CDS 50.0%
!! CATTTTCCCTTGGGGTGTCT+CGG - chr1_1:29899973-29899992 None:intergenic 50.0%
CAAGACCCAAAACCGTGTAG+TGG - chr1_1:29900021-29900040 None:intergenic 50.0%
!!! ACACGGTTTTGGGTCTTGAC+AGG + chr1_1:29900023-29900042 Msa0017890:CDS 50.0%
AGTCAGTTTGGCGACGTTGT+TGG - chr1_1:29900465-29900484 None:intergenic 50.0%
!! CGTCGCCAAACTGACTTGTA+AGG + chr1_1:29900469-29900488 Msa0017890:intron 50.0%
!! ACTGACTTGTAAGGCAAGGG+AGG + chr1_1:29900478-29900497 Msa0017890:intron 50.0%
! CCGTAGGCTTGGAAGTTTTG+TGG + chr1_1:29901740-29901759 Msa0017890:intron 50.0%
! GCAGACAGCATTTAGGACGA+AGG + chr1_1:29902081-29902100 Msa0017890:intron 50.0%
! TTCAGGGCCCATGGATTATC+AGG - chr1_1:29902309-29902328 None:intergenic 50.0%
TGATAATCCATGGGCCCTGA+AGG + chr1_1:29902308-29902327 Msa0017890:three_prime_UTR 50.0%
! GCGATTGCAAGAGGAGTACG+AGG + chr1_1:29899098-29899117 Msa0017890:CDS 55.0%
! GCAAGAGGAGTACGAGGAAG+AGG + chr1_1:29899104-29899123 Msa0017890:CDS 55.0%
CGAGACACCCCAAGGGAAAA+TGG + chr1_1:29899971-29899990 Msa0017890:CDS 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr1_1:29900853-29900872 None:intergenic 55.0%
ACTAAAGCTCCCGCATACGC+AGG - chr1_1:29900854-29900873 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr1_1:29900858-29900877 Msa0017890:intron 55.0%
! GGCCCATGGATTATCAGGCA+AGG - chr1_1:29902304-29902323 None:intergenic 55.0%
ATAGCATCCTTCAGGGCCCA+TGG - chr1_1:29902318-29902337 None:intergenic 55.0%
AGAACTCACGGCGAAGGTGG+TGG - chr1_1:29898576-29898595 None:intergenic 60.0%
CGTAGAACTCACGGCGAAGG+TGG - chr1_1:29898579-29898598 None:intergenic 60.0%
AGGCGTAGAACTCACGGCGA+AGG - chr1_1:29898582-29898601 None:intergenic 60.0%
CGAGGAAGAGGCTGAGAGAG+TGG + chr1_1:29899116-29899135 Msa0017890:CDS 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr1_1:29900826-29900845 Msa0017890:intron 60.0%
! AAATGGTGGGACCCCTTCCC+GGG + chr1_1:29900827-29900846 Msa0017890:intron 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr1_1:29900859-29900878 Msa0017890:intron 60.0%
! ACTCACGGCGAAGGTGGTGG+CGG - chr1_1:29898573-29898592 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr1_1:29900828-29900847 None:intergenic 65.0%
CATACGCAGGGCCCGGGAAG+GGG - chr1_1:29900841-29900860 None:intergenic 70.0%
GCATACGCAGGGCCCGGGAA+GGG - chr1_1:29900842-29900861 None:intergenic 70.0%
CTTCCCGGGCCCTGCGTATG+CGG + chr1_1:29900841-29900860 Msa0017890:intron 70.0%
TTCCCGGGCCCTGCGTATGC+GGG + chr1_1:29900842-29900861 Msa0017890:intron 70.0%
CGCATACGCAGGGCCCGGGA+AGG - chr1_1:29900843-29900862 None:intergenic 75.0%
CTCCCGCATACGCAGGGCCC+GGG - chr1_1:29900847-29900866 None:intergenic 75.0%
GCTCCCGCATACGCAGGGCC+CGG - chr1_1:29900848-29900867 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 29898310 29902595 29898310 ID=Msa0017890;Name=Msa0017890
chr1_1 mRNA 29898310 29902595 29898310 ID=Msa0017890-mRNA-1;Parent=Msa0017890;Name=Msa0017890-mRNA-1;_AED=0.07;_eAED=0.07;_QI=160|1|1|1|1|1|6|301|214
chr1_1 exon 29898310 29898586 29898310 ID=Msa0017890-mRNA-1:exon:9965;Parent=Msa0017890-mRNA-1
chr1_1 exon 29899030 29899137 29899030 ID=Msa0017890-mRNA-1:exon:9966;Parent=Msa0017890-mRNA-1
chr1_1 exon 29899800 29900044 29899800 ID=Msa0017890-mRNA-1:exon:9967;Parent=Msa0017890-mRNA-1
chr1_1 exon 29901062 29901098 29901062 ID=Msa0017890-mRNA-1:exon:9968;Parent=Msa0017890-mRNA-1
chr1_1 exon 29902085 29902142 29902085 ID=Msa0017890-mRNA-1:exon:9969;Parent=Msa0017890-mRNA-1
chr1_1 exon 29902215 29902595 29902215 ID=Msa0017890-mRNA-1:exon:9970;Parent=Msa0017890-mRNA-1
chr1_1 five_prime_UTR 29898310 29898469 29898310 ID=Msa0017890-mRNA-1:five_prime_utr;Parent=Msa0017890-mRNA-1
chr1_1 CDS 29898470 29898586 29898470 ID=Msa0017890-mRNA-1:cds;Parent=Msa0017890-mRNA-1
chr1_1 CDS 29899030 29899137 29899030 ID=Msa0017890-mRNA-1:cds;Parent=Msa0017890-mRNA-1
chr1_1 CDS 29899800 29900044 29899800 ID=Msa0017890-mRNA-1:cds;Parent=Msa0017890-mRNA-1
chr1_1 CDS 29901062 29901098 29901062 ID=Msa0017890-mRNA-1:cds;Parent=Msa0017890-mRNA-1
chr1_1 CDS 29902085 29902142 29902085 ID=Msa0017890-mRNA-1:cds;Parent=Msa0017890-mRNA-1
chr1_1 CDS 29902215 29902294 29902215 ID=Msa0017890-mRNA-1:cds;Parent=Msa0017890-mRNA-1
chr1_1 three_prime_UTR 29902295 29902595 29902295 ID=Msa0017890-mRNA-1:three_prime_utr;Parent=Msa0017890-mRNA-1
Gene Sequence

>Msa0017890

ATGAAGGAAGATGAAGAAGGTGATGATAGAAAAATCAACGAAGAGAAAGTTCAAGAATTCAAGCTTTGGGGAATTTTCCTCTTCGGTTTAATTGGTGCTACCGCCACCACCTTCGCCTTGTCCAGATCGAAAGGCGGTTCTTTTCGATCAGCTTTTCAAGAAGAAGCTTGGAAGCGGTACAATAAGCGATTGCAAGAGGAGTACGAGGAAGAGGCTGAGAGAGTGGAAAGAATAAGGCAAATGCAAAGTGTGTTTAATAGAGAAAGGGAGAAGACGTTTAGAAGAAAATACGAGAATTGGAAGGAAAATGGTTCTGGCACAAATCATCACCATTTCCAAAGAGATGATTGGTATTCGAAAGCTGAACAAACCTTTAGAGACCAATGGAAAAATCACCGAGACACCCCAAGGGAAAATGGAAGCATAAATTATTCATTAACACACCACTACACGGTTTTGGGTCTTGACAGGTCTAGAACAACACCATATACAGATGCTGAAATAAAGACAGCATTTAGGACGAAGGCAAAGCAGTATCATCCTGATCAGAACCAAGAGGATAAAGAGGCTGCTGAAGCTAAGTTCAAAGAAGTAATGGATTCATACGAGGCCATACAACAAGAAAGAAAGAATCATAGTCTGTAA
Protein sequence

>Msa0017890

MKEDEEGDDRKINEEKVQEFKLWGIFLFGLIGATATTFALSRSKGGSFRSAFQEEAWKRYNKRLQEEYEEEAERVERIRQMQSVFNREREKTFRRKYENWKENGSGTNHHHFQRDDWYSKAEQTFRDQWKNHRDTPRENGSINYSLTHHYTVLGLDRSRTTPYTDAEIKTAFRTKAKQYHPDQNQEDKEAAEAKFKEVMDSYEAIQQERKNHSL*