AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0581700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0581700 MtrunA17_Chr1g0152051 97.196 214 6 0 1 214 1 214 1.80e-155 429
Msa0581700 MtrunA17_Chr3g0135221 58.294 211 65 3 21 211 13 220 1.19e-69 212
Msa0581700 MtrunA17_Chr3g0135211 43.721 215 104 5 1 213 1 200 1.32e-48 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0581700 AT4G07990.2 57.944 214 79 6 5 210 4 214 4.29e-76 228
Msa0581700 AT4G07990.1 54.386 228 79 7 5 210 4 228 6.39e-72 218
Msa0581700 AT4G07990.3 55.385 195 76 6 5 191 4 195 1.05e-65 201

Find 69 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAGGAAAACCTTAATTT+TGG 0.106710 4_1:+87179102 Msa0581700:three_prime_UTR
GTAATAAATACCCAAAATTA+AGG 0.233384 4_1:-87179113 None:intergenic
AACACACCACTACACGGTTT+TGG 0.249771 4_1:+87176675 Msa0581700:CDS
CAGCAGCCTAATGTGTATTT+AGG 0.266150 4_1:+87179210 Msa0581700:three_prime_UTR
TCCCTTTATAGGAAAGAATA+AGG 0.267545 4_1:+87176452 Msa0581700:intron
TTTATTTCAGCATCTGTATA+TGG 0.277581 4_1:-87177760 None:intergenic
TCGAATACCAATCATCTCTT+TGG 0.278893 4_1:-87176573 None:intergenic
ATTCTTTCTTTCTTGTTGTA+TGG 0.287118 4_1:-87178957 None:intergenic
TTCAATACATAGCATCCTTC+AGG 0.293400 4_1:-87179020 None:intergenic
CGAGACACCCCAAGGGAAAA+TGG 0.304097 4_1:+87176634 Msa0581700:CDS
AACATAGAGATAGTGGTTAT+TGG 0.305304 4_1:+87174964 Msa0581700:five_prime_UTR
TTCAGGGCCCATGGATTATC+AGG 0.323315 4_1:-87179003 None:intergenic
TTAGAAGAAAATACGAGAAT+TGG 0.328205 4_1:+87176515 Msa0581700:CDS
CTCTTTCACAGACAGCATTT+AGG 0.330462 4_1:+87178756 Msa0581700:intron
AGTTCAAGAATTCAAGCTTT+GGG 0.342435 4_1:+87175083 Msa0581700:CDS
TACGAGAATTGGAAGGAAAA+TGG 0.359437 4_1:+87176526 Msa0581700:CDS
TTATCCTCTTGGTTCTGATC+AGG 0.372747 4_1:-87178800 None:intergenic
ATATACAGATGCTGAAATAA+AGG 0.398272 4_1:+87177762 Msa0581700:CDS
ACCAATCATCTCTTTGGAAA+TGG 0.406526 4_1:-87176567 None:intergenic
GGGTATTTATTACACAATAG+TGG 0.419416 4_1:+87179123 Msa0581700:three_prime_UTR
AATTGGAAGGAAAATGGTTC+TGG 0.420102 4_1:+87176532 Msa0581700:CDS
AAGACCTACCTTTATCCTCT+TGG 0.428497 4_1:-87178811 None:intergenic
AAGTTCAAGAATTCAAGCTT+TGG 0.435666 4_1:+87175082 Msa0581700:CDS
AAAGTGTGTTTAATAGAGAA+AGG 0.440344 4_1:+87176482 Msa0581700:CDS
ACCATTTCCAAAGAGATGAT+TGG 0.453905 4_1:+87176566 Msa0581700:CDS
GATCAGAACCAAGAGGATAA+AGG 0.457480 4_1:+87178803 Msa0581700:CDS
TTCAAGAAGAAGCGTGGAAA+AGG 0.467511 4_1:+87175724 Msa0581700:CDS
AATTGAGAACATAGAGATAG+TGG 0.471746 4_1:+87174957 Msa0581700:five_prime_UTR
AGATTTGATATAACTGAGAT+TGG 0.471898 4_1:+87174996 Msa0581700:five_prime_UTR
AATTGCGCCGATTATTCAAA+AGG 0.474087 4_1:+87174922 Msa0581700:five_prime_UTR
CACAATGGTGTTGTTATCAT+AGG 0.475563 4_1:+87179154 Msa0581700:three_prime_UTR
TCAATACATAGCATCCTTCA+GGG 0.485739 4_1:-87179019 None:intergenic
AGAAGTAATGGATTCATATG+AGG 0.488969 4_1:+87178934 Msa0581700:CDS
GCAAGAGGAGTACGAGGAAG+AGG 0.489481 4_1:+87175761 Msa0581700:CDS
AAAAGAACCGCCTTTCGACC+TGG 0.489496 4_1:-87175691 None:intergenic
TTCATACCTAAATACACATT+AGG 0.493459 4_1:-87179216 None:intergenic
CAACCTTGCCTGATAATCCA+TGG 0.498944 4_1:+87178995 Msa0581700:three_prime_UTR
AAGTGTGTTTAATAGAGAAA+GGG 0.502349 4_1:+87176483 Msa0581700:CDS
AGCTAAGTTCAAAGAAGTAA+TGG 0.518736 4_1:+87178922 Msa0581700:CDS
AGAACTCACGGCGAAGGTGG+TGG 0.531498 4_1:-87175139 None:intergenic
TTGTAGTTGTCCAGGTCGAA+AGG 0.543788 4_1:+87175681 Msa0581700:intron
CGGGAATCTCATGCAGCAGA+GGG 0.560876 4_1:+87174887 Msa0581700:five_prime_UTR
GTTCAAGAATTCAAGCTTTG+GGG 0.563262 4_1:+87175084 Msa0581700:CDS
ATAGCATCCTTCAGGGCCCA+TGG 0.566617 4_1:-87179012 None:intergenic
CGAGGAAGAGGCTGAAAGAG+TGG 0.570281 4_1:+87175773 Msa0581700:CDS
GTATTTAGGTATGAAACAAA+TGG 0.570641 4_1:+87179224 Msa0581700:three_prime_UTR
CTAAGGTTGATGTAGTCCAC+TGG 0.583757 4_1:+87179069 Msa0581700:three_prime_UTR
TGATAATCCATGGGCCCTGA+AGG 0.584636 4_1:+87179005 Msa0581700:three_prime_UTR
AACAAACCTTTAGAGACCAA+TGG 0.589429 4_1:+87176602 Msa0581700:CDS
ACTCACGGCGAAGGTGGTGG+CGG 0.589497 4_1:-87175136 None:intergenic
AACCTTGCCTGATAATCCAT+GGG 0.589677 4_1:+87178996 Msa0581700:three_prime_UTR
AGGCGTAGAACTCACGGCGA+AGG 0.592392 4_1:-87175145 None:intergenic
TTCATTAACACACCACTACA+CGG 0.593016 4_1:+87176669 Msa0581700:CDS
AAGAAAATACGAGAATTGGA+AGG 0.602692 4_1:+87176519 Msa0581700:CDS
ACGGGAATCTCATGCAGCAG+AGG 0.610869 4_1:+87174886 None:intergenic
ACAGACAGCATTTAGGACGA+AGG 0.620598 4_1:+87178763 Msa0581700:intron
GGCCCATGGATTATCAGGCA+AGG 0.631445 4_1:-87178998 None:intergenic
GTACAATAAGCGATTGCAAG+AGG 0.632219 4_1:+87175746 Msa0581700:CDS
TAAATGAAGGAAGACGAAGA+AGG 0.633327 4_1:+87175033 Msa0581700:exon
CGTAGAACTCACGGCGAAGG+TGG 0.635643 4_1:-87175142 None:intergenic
CAAGACCCAAAACCGTGTAG+TGG 0.644029 4_1:-87176681 None:intergenic
TAGCACCAATTAAGCCAAAG+AGG 0.646121 4_1:-87175113 None:intergenic
AAAATCACCGAGACACCCCA+AGG 0.648601 4_1:+87176626 Msa0581700:CDS
GCGATTGCAAGAGGAGTACG+AGG 0.657325 4_1:+87175755 Msa0581700:CDS
TAGTTGTCCAGGTCGAAAGG+CGG 0.658973 4_1:+87175684 Msa0581700:intron
AAATCACCGAGACACCCCAA+GGG 0.664599 4_1:+87176627 Msa0581700:CDS
CACTGGTATGCTGAAAACCA+AGG 0.671896 4_1:+87179086 Msa0581700:three_prime_UTR
TCATCCTGATCAGAACCAAG+AGG 0.690141 4_1:+87178796 Msa0581700:CDS
ATAGTGGTGTTGTATCACAA+TGG 0.785001 4_1:+87179139 Msa0581700:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTATTTTCATTTTTT+TGG + chr4_1:87175586-87175605 Msa0581700:intron 10.0%
!!! TAAATTTTTTTATTCTTTAC+AGG - chr4_1:87176841-87176860 None:intergenic 10.0%
!!! CACATTTTTTTTTAAAAAAA+AGG - chr4_1:87177576-87177595 None:intergenic 10.0%
!!! TTATTTGATTATTTTTGAAA+TGG + chr4_1:87179266-87179285 Msa0581700:three_prime_UTR 10.0%
!!! TGATTTTTTTTTCTAAATGA+AGG + chr4_1:87175020-87175039 Msa0581700:exon 15.0%
!!! TTTTTATTTTACGTCATTTT+AGG + chr4_1:87175223-87175242 Msa0581700:intron 15.0%
!!! CTAAATATAAAAGCATAATA+TGG + chr4_1:87177069-87177088 Msa0581700:intron 15.0%
!! GAAAATGAAAACGAAATAAA+TGG - chr4_1:87175202-87175221 None:intergenic 20.0%
!! TATATGTGACTAATCATAAA+TGG - chr4_1:87176362-87176381 None:intergenic 20.0%
!! ATATCAGAATGCTAAAATAT+AGG + chr4_1:87176762-87176781 Msa0581700:intron 20.0%
!!! ATAACAATAACTTTTCTAAG+TGG + chr4_1:87177312-87177331 Msa0581700:intron 20.0%
!!! ATAAGAATAACTTTTCTAAG+TGG + chr4_1:87177409-87177428 Msa0581700:intron 20.0%
!! TATAATTTACAAACATCTTG+TGG - chr4_1:87178069-87178088 None:intergenic 20.0%
!!! ATTATACCAACTATTTTGAT+TGG + chr4_1:87178083-87178102 Msa0581700:intron 20.0%
!! AACATACTTATTATGATTAG+TGG + chr4_1:87178484-87178503 Msa0581700:intron 20.0%
!! ACATACTTATTATGATTAGT+GGG + chr4_1:87178485-87178504 Msa0581700:intron 20.0%
!! CATTATTACATAATGATTCA+TGG + chr4_1:87178872-87178891 Msa0581700:intron 20.0%
!!! GAAAATAAGCATAATTTTTG+AGG + chr4_1:87179040-87179059 Msa0581700:three_prime_UTR 20.0%
!! GTAATAAATACCCAAAATTA+AGG - chr4_1:87179116-87179135 None:intergenic 20.0%
! AGATTTGATATAACTGAGAT+TGG + chr4_1:87174996-87175015 Msa0581700:five_prime_UTR 25.0%
! GAAAATGAAAAGAACAAAGA+AGG - chr4_1:87175168-87175187 None:intergenic 25.0%
! ACTCTATATTCTGTTAACAT+GGG + chr4_1:87175557-87175576 Msa0581700:intron 25.0%
!!! GATGTTACGTTATTTTTAGA+CGG - chr4_1:87175907-87175926 None:intergenic 25.0%
!! TATCATGTGATCTTTGAAAA+TGG + chr4_1:87175932-87175951 Msa0581700:intron 25.0%
!! ATCATGTGATCTTTGAAAAT+GGG + chr4_1:87175933-87175952 Msa0581700:intron 25.0%
!! AAAATTTGCTTTGTCACAAA+AGG - chr4_1:87175965-87175984 None:intergenic 25.0%
!! AATCTCTATTAGTTGATTCT+AGG + chr4_1:87175990-87176009 Msa0581700:intron 25.0%
!! TATTAGTTGATTCTAGGAAT+TGG + chr4_1:87175996-87176015 Msa0581700:intron 25.0%
! AAAGTGTGTTTAATAGAGAA+AGG + chr4_1:87176482-87176501 Msa0581700:CDS 25.0%
! AAGTGTGTTTAATAGAGAAA+GGG + chr4_1:87176483-87176502 Msa0581700:CDS 25.0%
! TTAGAAGAAAATACGAGAAT+TGG + chr4_1:87176515-87176534 Msa0581700:CDS 25.0%
! TTCTAAGTGGATATATTGTA+TGG + chr4_1:87177325-87177344 Msa0581700:intron 25.0%
! TTCTAAGTGGATATATTGTA+TGG + chr4_1:87177325-87177344 Msa0581700:intron 25.0%
!! TTTGAAGCAGTATATGATTA+TGG + chr4_1:87177478-87177497 Msa0581700:intron 25.0%
! TTTATTTCAGCATCTGTATA+TGG - chr4_1:87177763-87177782 None:intergenic 25.0%
! ATATACAGATGCTGAAATAA+AGG + chr4_1:87177762-87177781 Msa0581700:CDS 25.0%
!! CTCTTTTTAACACAATATCT+TGG + chr4_1:87177797-87177816 Msa0581700:intron 25.0%
! CATATGATAAATATGACAGA+GGG - chr4_1:87177880-87177899 None:intergenic 25.0%
!!! TTTTCGTTGATGTTATTTGA+AGG + chr4_1:87177914-87177933 Msa0581700:intron 25.0%
!!! TGAAATGCTCTAAAGTATAA+AGG + chr4_1:87178145-87178164 Msa0581700:intron 25.0%
! TCAAATATATTCAGCCTATA+CGG + chr4_1:87178176-87178195 Msa0581700:intron 25.0%
! AACTCAGAAATGTAAATATG+TGG + chr4_1:87178245-87178264 Msa0581700:intron 25.0%
! ACTCAGAAATGTAAATATGT+GGG + chr4_1:87178246-87178265 Msa0581700:intron 25.0%
!!! TTTGACCGGTTTTATATAAA+TGG - chr4_1:87178367-87178386 None:intergenic 25.0%
! ATTTATATAAAACCGGTCAA+AGG + chr4_1:87178366-87178385 Msa0581700:intron 25.0%
! TTTATATAAAACCGGTCAAA+GGG + chr4_1:87178367-87178386 Msa0581700:intron 25.0%
! TACAGAAAATCAAGGTAAAA+AGG - chr4_1:87178609-87178628 None:intergenic 25.0%
! GTAAGTATTACAGAAAATCA+AGG - chr4_1:87178617-87178636 None:intergenic 25.0%
!! TTTGACCTTTCTTTCATTTT+CGG + chr4_1:87178696-87178715 Msa0581700:intron 25.0%
! TATTACCGAAAATGAAAGAA+AGG - chr4_1:87178704-87178723 None:intergenic 25.0%
!! ATTCTTTCTTTCTTGTTGTA+TGG - chr4_1:87178960-87178979 None:intergenic 25.0%
! TTCATACCTAAATACACATT+AGG - chr4_1:87179219-87179238 None:intergenic 25.0%
! GTATTTAGGTATGAAACAAA+TGG + chr4_1:87179224-87179243 Msa0581700:three_prime_UTR 25.0%
! GCTTTCTCCTTTTGAATAAT+CGG - chr4_1:87174932-87174951 None:intergenic 30.0%
AATTGAGAACATAGAGATAG+TGG + chr4_1:87174957-87174976 Msa0581700:five_prime_UTR 30.0%
! AACATAGAGATAGTGGTTAT+TGG + chr4_1:87174964-87174983 Msa0581700:five_prime_UTR 30.0%
AAGTTCAAGAATTCAAGCTT+TGG + chr4_1:87175082-87175101 Msa0581700:CDS 30.0%
AGTTCAAGAATTCAAGCTTT+GGG + chr4_1:87175083-87175102 Msa0581700:CDS 30.0%
!! ATTTTCCTCTTTGGCTTAAT+TGG + chr4_1:87175108-87175127 Msa0581700:CDS 30.0%
CGCTTATCATTCATGATAAA+AGG - chr4_1:87175285-87175304 None:intergenic 30.0%
! TTTTCATAAGTTCTGCCAAA+TGG + chr4_1:87175482-87175501 Msa0581700:intron 30.0%
AACAGAATATAGAGTCTCTT+TGG - chr4_1:87175552-87175571 None:intergenic 30.0%
GACTCTATATTCTGTTAACA+TGG + chr4_1:87175556-87175575 Msa0581700:intron 30.0%
!! ACCTGATTTTATTGTTGTTG+TGG + chr4_1:87175840-87175859 Msa0581700:intron 30.0%
ACCACAACAACAATAAAATC+AGG - chr4_1:87175844-87175863 None:intergenic 30.0%
ACATTCCGAACTTAAATCAA+CGG + chr4_1:87176113-87176132 Msa0581700:intron 30.0%
TAAGTGAAGTGACTATCATA+AGG - chr4_1:87176285-87176304 None:intergenic 30.0%
! TGTTTCTATCTTCCCTTTAT+AGG + chr4_1:87176441-87176460 Msa0581700:intron 30.0%
TCCCTTTATAGGAAAGAATA+AGG + chr4_1:87176452-87176471 Msa0581700:intron 30.0%
! GCCTTATTCTTTCCTATAAA+GGG - chr4_1:87176456-87176475 None:intergenic 30.0%
! TGCCTTATTCTTTCCTATAA+AGG - chr4_1:87176457-87176476 None:intergenic 30.0%
AAGAAAATACGAGAATTGGA+AGG + chr4_1:87176519-87176538 Msa0581700:CDS 30.0%
!! ATTTTTCCATTGGTCTCTAA+AGG - chr4_1:87176611-87176630 None:intergenic 30.0%
TTTAAACAAGTGCAACAACA+TGG - chr4_1:87176877-87176896 None:intergenic 30.0%
TCAGATCATTGTAGCAATTA+AGG - chr4_1:87176958-87176977 None:intergenic 30.0%
ACCTGAAAATGTGTTATAAG+TGG + chr4_1:87177000-87177019 Msa0581700:intron 30.0%
CCTGAAAATGTGTTATAAGT+GGG + chr4_1:87177001-87177020 Msa0581700:intron 30.0%
TACATAACTGGATTGTTCTA+GGG - chr4_1:87177036-87177055 None:intergenic 30.0%
TTACATAACTGGATTGTTCT+AGG - chr4_1:87177037-87177056 None:intergenic 30.0%
CTAACTCATTCTTACATAAC+TGG - chr4_1:87177048-87177067 None:intergenic 30.0%
CAGTTATGTAAGAATGAGTT+AGG + chr4_1:87177046-87177065 Msa0581700:intron 30.0%
AAATATGCTTATATAGTGGC+GGG - chr4_1:87177193-87177212 None:intergenic 30.0%
CAAATATGCTTATATAGTGG+CGG - chr4_1:87177194-87177213 None:intergenic 30.0%
CTGCAAATATGCTTATATAG+TGG - chr4_1:87177197-87177216 None:intergenic 30.0%
ATATTGTATGGTACATCTAG+AGG + chr4_1:87177337-87177356 Msa0581700:intron 30.0%
TATTGTATGGTACATCTAGA+GGG + chr4_1:87177338-87177357 Msa0581700:intron 30.0%
!! TTTTAAAAAAAAGGGCAACC+CGG - chr4_1:87177567-87177586 None:intergenic 30.0%
ACGGTATATGATTAAAGCAA+GGG - chr4_1:87177647-87177666 None:intergenic 30.0%
ACAATATCTTGGCATATTAC+TGG + chr4_1:87177808-87177827 Msa0581700:intron 30.0%
CCATATGATAAATATGACAG+AGG - chr4_1:87177881-87177900 None:intergenic 30.0%
CCTCTGTCATATTTATCATA+TGG + chr4_1:87177878-87177897 Msa0581700:intron 30.0%
TTAACTAGTGTCCAACAAAA+CGG - chr4_1:87177991-87178010 None:intergenic 30.0%
!! TATACTTTAGAGCATTTCAC+AGG - chr4_1:87178144-87178163 None:intergenic 30.0%
! CTCTGCCATTTATATAAAAC+CGG + chr4_1:87178359-87178378 Msa0581700:intron 30.0%
AGCTAAGTTCAAAGAAGTAA+TGG + chr4_1:87178922-87178941 Msa0581700:CDS 30.0%
!! AGAAGTAATGGATTCATATG+AGG + chr4_1:87178934-87178953 Msa0581700:CDS 30.0%
!!! AAGCATAATTTTTGAGGTTG+TGG + chr4_1:87179046-87179065 Msa0581700:three_prime_UTR 30.0%
! ACCAAGGAAAACCTTAATTT+TGG + chr4_1:87179102-87179121 Msa0581700:three_prime_UTR 30.0%
! CCAAGGAAAACCTTAATTTT+GGG + chr4_1:87179103-87179122 Msa0581700:three_prime_UTR 30.0%
GGGTATTTATTACACAATAG+TGG + chr4_1:87179123-87179142 Msa0581700:three_prime_UTR 30.0%
!! AATTGCGCCGATTATTCAAA+AGG + chr4_1:87174922-87174941 Msa0581700:five_prime_UTR 35.0%
TAAATGAAGGAAGACGAAGA+AGG + chr4_1:87175033-87175052 Msa0581700:exon 35.0%
GTTCAAGAATTCAAGCTTTG+GGG + chr4_1:87175084-87175103 Msa0581700:CDS 35.0%
! GACATGTCACAAACTGTTTT+CGG - chr4_1:87175454-87175473 None:intergenic 35.0%
TAAACACTTGTGAGACCATT+TGG - chr4_1:87175500-87175519 None:intergenic 35.0%
! TGAACTTTTTGTAGTTGTCC+AGG + chr4_1:87175673-87175692 Msa0581700:intron 35.0%
! ATGTGATCTTTGAAAATGGG+AGG + chr4_1:87175936-87175955 Msa0581700:intron 35.0%
!! CTCAACCGTTGATTTAAGTT+CGG - chr4_1:87176121-87176140 None:intergenic 35.0%
ATGAACTACCACATGAAACT+AGG + chr4_1:87176141-87176160 Msa0581700:intron 35.0%
TGAAATCACCTAGTTTCATG+TGG - chr4_1:87176152-87176171 None:intergenic 35.0%
TGCTTAGATGTTTCATTGCT+TGG + chr4_1:87176232-87176251 Msa0581700:intron 35.0%
TCATAAGTGACTATTACACG+TGG - chr4_1:87176321-87176340 None:intergenic 35.0%
TCAGAAGTGACTATCATTTG+TGG - chr4_1:87176405-87176424 None:intergenic 35.0%
TCACTTCTGATAAGTCGTTT+AGG + chr4_1:87176415-87176434 Msa0581700:intron 35.0%
TACGAGAATTGGAAGGAAAA+TGG + chr4_1:87176526-87176545 Msa0581700:CDS 35.0%
AATTGGAAGGAAAATGGTTC+TGG + chr4_1:87176532-87176551 Msa0581700:CDS 35.0%
ACCATTTCCAAAGAGATGAT+TGG + chr4_1:87176566-87176585 Msa0581700:CDS 35.0%
! ACCAATCATCTCTTTGGAAA+TGG - chr4_1:87176570-87176589 None:intergenic 35.0%
TCGAATACCAATCATCTCTT+TGG - chr4_1:87176576-87176595 None:intergenic 35.0%
! AACAAACCTTTAGAGACCAA+TGG + chr4_1:87176602-87176621 Msa0581700:CDS 35.0%
! TTATGCTTCCATTTTCCCTT+GGG - chr4_1:87176645-87176664 None:intergenic 35.0%
! TTTATGCTTCCATTTTCCCT+TGG - chr4_1:87176646-87176665 None:intergenic 35.0%
TTCATTAACACACCACTACA+CGG + chr4_1:87176669-87176688 Msa0581700:CDS 35.0%
! CCCACTTATAACACATTTTC+AGG - chr4_1:87177004-87177023 None:intergenic 35.0%
TGATATAATGTCTGTCCCAA+GGG - chr4_1:87177115-87177134 None:intergenic 35.0%
ATGGCCCAATAAAAACCAAA+TGG + chr4_1:87177497-87177516 Msa0581700:intron 35.0%
!!! CCCACCATTTGGTTTTTATT+GGG - chr4_1:87177504-87177523 None:intergenic 35.0%
CCCAATAAAAACCAAATGGT+GGG + chr4_1:87177501-87177520 Msa0581700:intron 35.0%
!!! TCCCACCATTTGGTTTTTAT+TGG - chr4_1:87177505-87177524 None:intergenic 35.0%
CGGTATATGATTAAAGCAAG+GGG - chr4_1:87177646-87177665 None:intergenic 35.0%
GACGGTATATGATTAAAGCA+AGG - chr4_1:87177648-87177667 None:intergenic 35.0%
! TTGCTAACTTTTGATGCAGA+CGG - chr4_1:87177666-87177685 None:intergenic 35.0%
!! TCCTTCTTTTGATTGATCTC+AGG + chr4_1:87177725-87177744 Msa0581700:intron 35.0%
ACCTGAGATCAATCAAAAGA+AGG - chr4_1:87177729-87177748 None:intergenic 35.0%
AAATATGACAGAGGGTGATA+GGG - chr4_1:87177872-87177891 None:intergenic 35.0%
TAAATATGACAGAGGGTGAT+AGG - chr4_1:87177873-87177892 None:intergenic 35.0%
! CGTTGATGTTATTTGAAGGA+TGG + chr4_1:87177918-87177937 Msa0581700:intron 35.0%
!!! CTAGTTTTGTACCGTTTTGT+TGG + chr4_1:87177977-87177996 Msa0581700:intron 35.0%
CGAATGCCAATCAAAATAGT+TGG - chr4_1:87178092-87178111 None:intergenic 35.0%
TTCGACAAAATCCTATGGTA+TGG + chr4_1:87178108-87178127 Msa0581700:intron 35.0%
! AGAGCATTTCACAGGTAATT+TGG - chr4_1:87178136-87178155 None:intergenic 35.0%
ATATATTCAGCCTATACGGA+TGG + chr4_1:87178180-87178199 Msa0581700:intron 35.0%
TACATTTCTGAGTTACGTCA+CGG - chr4_1:87178239-87178258 None:intergenic 35.0%
GAAGAGTGAAAAAAATACGC+AGG - chr4_1:87178273-87178292 None:intergenic 35.0%
GAGAGAAAGAGAAGAGAAAT+GGG - chr4_1:87178329-87178348 None:intergenic 35.0%
AGAGAGAAAGAGAAGAGAAA+TGG - chr4_1:87178330-87178349 None:intergenic 35.0%
AATATCTTGTACCCTTTGAC+CGG - chr4_1:87178381-87178400 None:intergenic 35.0%
AGGGTACAAGATATTGATGA+TGG + chr4_1:87178386-87178405 Msa0581700:intron 35.0%
! GGGTACAAGATATTGATGAT+GGG + chr4_1:87178387-87178406 Msa0581700:intron 35.0%
!! GGTACAAGATATTGATGATG+GGG + chr4_1:87178388-87178407 Msa0581700:intron 35.0%
TCAATACATAGCATCCTTCA+GGG - chr4_1:87179022-87179041 None:intergenic 35.0%
TTCAATACATAGCATCCTTC+AGG - chr4_1:87179023-87179042 None:intergenic 35.0%
!!! AATTTTTGAGGTTGTGGCTA+AGG + chr4_1:87179052-87179071 Msa0581700:three_prime_UTR 35.0%
! CCCAAAATTAAGGTTTTCCT+TGG - chr4_1:87179106-87179125 None:intergenic 35.0%
!! ATAGTGGTGTTGTATCACAA+TGG + chr4_1:87179139-87179158 Msa0581700:three_prime_UTR 35.0%
!! CACAATGGTGTTGTTATCAT+AGG + chr4_1:87179154-87179173 Msa0581700:three_prime_UTR 35.0%
!!! GGAGCCATTGATTTTTATGT+CGG + chr4_1:87179175-87179194 Msa0581700:three_prime_UTR 35.0%
!!! GAGCCATTGATTTTTATGTC+GGG + chr4_1:87179176-87179195 Msa0581700:three_prime_UTR 35.0%
GTTCCCGACATAAAAATCAA+TGG - chr4_1:87179182-87179201 None:intergenic 35.0%
!! CTTTGGGGAATTTTCCTCTT+TGG + chr4_1:87175099-87175118 Msa0581700:CDS 40.0%
TAGCACCAATTAAGCCAAAG+AGG - chr4_1:87175116-87175135 None:intergenic 40.0%
! TTCAAGAAGAAGCGTGGAAA+AGG + chr4_1:87175724-87175743 Msa0581700:CDS 40.0%
GTACAATAAGCGATTGCAAG+AGG + chr4_1:87175746-87175765 Msa0581700:CDS 40.0%
AGACGTATGATCTAGATCGA+CGG + chr4_1:87176049-87176068 Msa0581700:intron 40.0%
ACTAGGTGATTTCAAACTGC+AGG + chr4_1:87176158-87176177 Msa0581700:intron 40.0%
TTCATTGCTTGGTTAGTGCT+AGG + chr4_1:87176243-87176262 Msa0581700:intron 40.0%
! TGTCTCGGTGATTTTTCCAT+TGG - chr4_1:87176621-87176640 None:intergenic 40.0%
! TATGCTTCCATTTTCCCTTG+GGG - chr4_1:87176644-87176663 None:intergenic 40.0%
AGAGTCTATTCAAGATCCCT+TGG + chr4_1:87177096-87177115 Msa0581700:intron 40.0%
GAGTCTATTCAAGATCCCTT+GGG + chr4_1:87177097-87177116 Msa0581700:intron 40.0%
CTGATATAATGTCTGTCCCA+AGG - chr4_1:87177116-87177135 None:intergenic 40.0%
CTTGGGACAGACATTATATC+AGG + chr4_1:87177114-87177133 Msa0581700:intron 40.0%
AGACATTATATCAGGCACTC+AGG + chr4_1:87177122-87177141 Msa0581700:intron 40.0%
ATTATATCAGGCACTCAGGT+TGG + chr4_1:87177126-87177145 Msa0581700:intron 40.0%
TTATATCAGGCACTCAGGTT+GGG + chr4_1:87177127-87177146 Msa0581700:intron 40.0%
GACCATATCTCACTCAATGT+AGG + chr4_1:87177216-87177235 Msa0581700:intron 40.0%
AGCCTACATTGAGTGAGATA+TGG - chr4_1:87177221-87177240 None:intergenic 40.0%
GCCCAATAAAAACCAAATGG+TGG + chr4_1:87177500-87177519 Msa0581700:intron 40.0%
AATATGACAGAGGGTGATAG+GGG - chr4_1:87177871-87177890 None:intergenic 40.0%
TGGCATTCGACAAAATCCTA+TGG + chr4_1:87178103-87178122 Msa0581700:intron 40.0%
GTAATTTGGCACCATACCAT+AGG - chr4_1:87178122-87178141 None:intergenic 40.0%
TTCTCACAATCCATCCGTAT+AGG - chr4_1:87178193-87178212 None:intergenic 40.0%
CAATAAATAGTCCGTAGGCT+TGG + chr4_1:87178411-87178430 Msa0581700:intron 40.0%
CTCTTTCACAGACAGCATTT+AGG + chr4_1:87178756-87178775 Msa0581700:intron 40.0%
!! TTATCCTCTTGGTTCTGATC+AGG - chr4_1:87178803-87178822 None:intergenic 40.0%
GATCAGAACCAAGAGGATAA+AGG + chr4_1:87178803-87178822 Msa0581700:CDS 40.0%
AGAACCAAGAGGATAAAGGT+AGG + chr4_1:87178807-87178826 Msa0581700:intron 40.0%
AAGACCTACCTTTATCCTCT+TGG - chr4_1:87178814-87178833 None:intergenic 40.0%
TGGATGTGAAAATGTGACAG+AGG + chr4_1:87178892-87178911 Msa0581700:intron 40.0%
AACCTTGCCTGATAATCCAT+GGG + chr4_1:87178996-87179015 Msa0581700:three_prime_UTR 40.0%
CAGCAGCCTAATGTGTATTT+AGG + chr4_1:87179210-87179229 Msa0581700:three_prime_UTR 40.0%
! CAAAGAAGGCGTAGAACTCA+CGG - chr4_1:87175154-87175173 None:intergenic 45.0%
TTGTAGTTGTCCAGGTCGAA+AGG + chr4_1:87175681-87175700 Msa0581700:intron 45.0%
!! CAGCTTTTCAAGAAGAAGCG+TGG + chr4_1:87175718-87175737 Msa0581700:CDS 45.0%
TGTGACACGCAGTTACAATG+GGG - chr4_1:87176025-87176044 None:intergenic 45.0%
CTGTGACACGCAGTTACAAT+GGG - chr4_1:87176026-87176045 None:intergenic 45.0%
TGCAGTAAGCTGCAAAGAAG+TGG + chr4_1:87176199-87176218 Msa0581700:intron 45.0%
! AACACACCACTACACGGTTT+TGG + chr4_1:87176675-87176694 Msa0581700:CDS 45.0%
! ACACACCACTACACGGTTTT+GGG + chr4_1:87176676-87176695 Msa0581700:CDS 45.0%
! TATATCAGGCACTCAGGTTG+GGG + chr4_1:87177128-87177147 Msa0581700:intron 45.0%
!! CAAACTGACTTGTAAGGCGA+GGG + chr4_1:87177164-87177183 Msa0581700:intron 45.0%
TGGGGCAATAAATAGTCCGT+AGG + chr4_1:87178406-87178425 Msa0581700:intron 45.0%
CCACAAAACTTCCAAGCCTA+CGG - chr4_1:87178425-87178444 None:intergenic 45.0%
! ACAGACAGCATTTAGGACGA+AGG + chr4_1:87178763-87178782 Msa0581700:intron 45.0%
TCATCCTGATCAGAACCAAG+AGG + chr4_1:87178796-87178815 Msa0581700:CDS 45.0%
CAACCTTGCCTGATAATCCA+TGG + chr4_1:87178995-87179014 Msa0581700:three_prime_UTR 45.0%
!! CTAAGGTTGATGTAGTCCAC+TGG + chr4_1:87179069-87179088 Msa0581700:three_prime_UTR 45.0%
!! CTTGGTTTTCAGCATACCAG+TGG - chr4_1:87179088-87179107 None:intergenic 45.0%
CACTGGTATGCTGAAAACCA+AGG + chr4_1:87179086-87179105 Msa0581700:three_prime_UTR 45.0%
!!! ACATTTTTTTTTAAAAAAAA+GGG - chr4_1:87177575-87177594 None:intergenic 5.0%
!!! TTTTATTTAAGAAATAATAA+TGG - chr4_1:87177955-87177974 None:intergenic 5.0%
TAGTTGTCCAGGTCGAAAGG+CGG + chr4_1:87175684-87175703 Msa0581700:intron 50.0%
AAAAGAACCGCCTTTCGACC+TGG - chr4_1:87175694-87175713 None:intergenic 50.0%
GCTGTGACACGCAGTTACAA+TGG - chr4_1:87176027-87176046 None:intergenic 50.0%
AAAATCACCGAGACACCCCA+AGG + chr4_1:87176626-87176645 Msa0581700:CDS 50.0%
AAATCACCGAGACACCCCAA+GGG + chr4_1:87176627-87176646 Msa0581700:CDS 50.0%
!! CATTTTCCCTTGGGGTGTCT+CGG - chr4_1:87176636-87176655 None:intergenic 50.0%
CAAGACCCAAAACCGTGTAG+TGG - chr4_1:87176684-87176703 None:intergenic 50.0%
!!! ACACGGTTTTGGGTCTTGAC+AGG + chr4_1:87176686-87176705 Msa0581700:CDS 50.0%
AGTCAGTTTGGCGACGTTGT+TGG - chr4_1:87177154-87177173 None:intergenic 50.0%
!! CGTCGCCAAACTGACTTGTA+AGG + chr4_1:87177158-87177177 Msa0581700:intron 50.0%
CCTCGCCTTACAAGTCAGTT+TGG - chr4_1:87177166-87177185 None:intergenic 50.0%
!! CCAAACTGACTTGTAAGGCG+AGG + chr4_1:87177163-87177182 Msa0581700:intron 50.0%
! CCGTAGGCTTGGAAGTTTTG+TGG + chr4_1:87178422-87178441 Msa0581700:intron 50.0%
! TTCAGGGCCCATGGATTATC+AGG - chr4_1:87179006-87179025 None:intergenic 50.0%
TGATAATCCATGGGCCCTGA+AGG + chr4_1:87179005-87179024 Msa0581700:three_prime_UTR 50.0%
CGGGAATCTCATGCAGCAGA+GGG + chr4_1:87174887-87174906 Msa0581700:five_prime_UTR 55.0%
! GCGATTGCAAGAGGAGTACG+AGG + chr4_1:87175755-87175774 Msa0581700:CDS 55.0%
! GCAAGAGGAGTACGAGGAAG+AGG + chr4_1:87175761-87175780 Msa0581700:CDS 55.0%
CGAGGAAGAGGCTGAAAGAG+TGG + chr4_1:87175773-87175792 Msa0581700:CDS 55.0%
CGAGACACCCCAAGGGAAAA+TGG + chr4_1:87176634-87176653 Msa0581700:CDS 55.0%
!! ACTGACTTGTAAGGCGAGGG+AGG + chr4_1:87177167-87177186 Msa0581700:intron 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr4_1:87177540-87177559 None:intergenic 55.0%
ACTAAAGCTCCCGCATACGC+AGG - chr4_1:87177541-87177560 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr4_1:87177545-87177564 Msa0581700:intron 55.0%
! GGCCCATGGATTATCAGGCA+AGG - chr4_1:87179001-87179020 None:intergenic 55.0%
ATAGCATCCTTCAGGGCCCA+TGG - chr4_1:87179015-87179034 None:intergenic 55.0%
AGAACTCACGGCGAAGGTGG+TGG - chr4_1:87175142-87175161 None:intergenic 60.0%
CGTAGAACTCACGGCGAAGG+TGG - chr4_1:87175145-87175164 None:intergenic 60.0%
AGGCGTAGAACTCACGGCGA+AGG - chr4_1:87175148-87175167 None:intergenic 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr4_1:87177513-87177532 Msa0581700:intron 60.0%
! AAATGGTGGGACCCCTTCCC+GGG + chr4_1:87177514-87177533 Msa0581700:intron 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr4_1:87177546-87177565 Msa0581700:intron 60.0%
! ACTCACGGCGAAGGTGGTGG+CGG - chr4_1:87175139-87175158 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr4_1:87177515-87177534 None:intergenic 65.0%
CATACGCAGGGCCCGGGAAG+GGG - chr4_1:87177528-87177547 None:intergenic 70.0%
GCATACGCAGGGCCCGGGAA+GGG - chr4_1:87177529-87177548 None:intergenic 70.0%
CTTCCCGGGCCCTGCGTATG+CGG + chr4_1:87177528-87177547 Msa0581700:intron 70.0%
TTCCCGGGCCCTGCGTATGC+GGG + chr4_1:87177529-87177548 Msa0581700:intron 70.0%
CGCATACGCAGGGCCCGGGA+AGG - chr4_1:87177530-87177549 None:intergenic 75.0%
CTCCCGCATACGCAGGGCCC+GGG - chr4_1:87177534-87177553 None:intergenic 75.0%
GCTCCCGCATACGCAGGGCC+CGG - chr4_1:87177535-87177554 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 87174887 87179292 87174887 ID=Msa0581700;Name=Msa0581700
chr4_1 mRNA 87174887 87179292 87174887 ID=Msa0581700-mRNA-1;Parent=Msa0581700;Name=Msa0581700-mRNA-1;_AED=0.09;_eAED=0.09;_QI=149|1|1|1|1|1|6|301|214
chr4_1 exon 87174887 87175152 87174887 ID=Msa0581700-mRNA-1:exon:32328;Parent=Msa0581700-mRNA-1
chr4_1 exon 87175687 87175794 87175687 ID=Msa0581700-mRNA-1:exon:32329;Parent=Msa0581700-mRNA-1
chr4_1 exon 87176463 87176707 87176463 ID=Msa0581700-mRNA-1:exon:32330;Parent=Msa0581700-mRNA-1
chr4_1 exon 87177747 87177783 87177747 ID=Msa0581700-mRNA-1:exon:32331;Parent=Msa0581700-mRNA-1
chr4_1 exon 87178767 87178824 87178767 ID=Msa0581700-mRNA-1:exon:32332;Parent=Msa0581700-mRNA-1
chr4_1 exon 87178912 87179292 87178912 ID=Msa0581700-mRNA-1:exon:32333;Parent=Msa0581700-mRNA-1
chr4_1 five_prime_UTR 87174887 87175035 87174887 ID=Msa0581700-mRNA-1:five_prime_utr;Parent=Msa0581700-mRNA-1
chr4_1 CDS 87175036 87175152 87175036 ID=Msa0581700-mRNA-1:cds;Parent=Msa0581700-mRNA-1
chr4_1 CDS 87175687 87175794 87175687 ID=Msa0581700-mRNA-1:cds;Parent=Msa0581700-mRNA-1
chr4_1 CDS 87176463 87176707 87176463 ID=Msa0581700-mRNA-1:cds;Parent=Msa0581700-mRNA-1
chr4_1 CDS 87177747 87177783 87177747 ID=Msa0581700-mRNA-1:cds;Parent=Msa0581700-mRNA-1
chr4_1 CDS 87178767 87178824 87178767 ID=Msa0581700-mRNA-1:cds;Parent=Msa0581700-mRNA-1
chr4_1 CDS 87178912 87178991 87178912 ID=Msa0581700-mRNA-1:cds;Parent=Msa0581700-mRNA-1
chr4_1 three_prime_UTR 87178992 87179292 87178992 ID=Msa0581700-mRNA-1:three_prime_utr;Parent=Msa0581700-mRNA-1
Gene Sequence

>Msa0581700

ATGAAGGAAGACGAAGAAGGTGATGATAGAAAAATCAACGAAGAGAAAGTTCAAGAATTCAAGCTTTGGGGAATTTTCCTCTTTGGCTTAATTGGTGCTACCGCCACCACCTTCGCCTTGTCCAGGTCGAAAGGCGGTTCTTTTCGATCAGCTTTTCAAGAAGAAGCGTGGAAAAGGTACAATAAGCGATTGCAAGAGGAGTACGAGGAAGAGGCTGAAAGAGTGGAAAGAATAAGGCAAATGCAAAGTGTGTTTAATAGAGAAAGGGAGAAGACGTTTAGAAGAAAATACGAGAATTGGAAGGAAAATGGTTCTGGCACAAATCATCACCATTTCCAAAGAGATGATTGGTATTCGAAAGCTGAACAAACCTTTAGAGACCAATGGAAAAATCACCGAGACACCCCAAGGGAAAATGGAAGCATAAATTATTCATTAACACACCACTACACGGTTTTGGGTCTTGACAGGTCTAGAACAACACCATATACAGATGCTGAAATAAAGACAGCATTTAGGACGAAGGCAAAGCAGTATCATCCTGATCAGAACCAAGAGGATAAAGAGGCTGCTGAAGCTAAGTTCAAAGAAGTAATGGATTCATATGAGGCCATACAACAAGAAAGAAAGAATCATAGTCTGTAA
Protein sequence

>Msa0581700

MKEDEEGDDRKINEEKVQEFKLWGIFLFGLIGATATTFALSRSKGGSFRSAFQEEAWKRYNKRLQEEYEEEAERVERIRQMQSVFNREREKTFRRKYENWKENGSGTNHHHFQRDDWYSKAEQTFRDQWKNHRDTPRENGSINYSLTHHYTVLGLDRSRTTPYTDAEIKTAFRTKAKQYHPDQNQEDKEAAEAKFKEVMDSYEAIQQERKNHSL*