AlfalfaGEDB | Msa0030720

M. sativa cultivar zhongmu-4 / Msa0030720

KEGG: -
GO: -
PFAM: PF07279.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030720	Msa0078100	0.850807	1.336909e-60	-8.615850e-47
Msa0030720	Msa0171980	0.807979	3.878858e-50	-8.615850e-47
Msa0030720	Msa0638540	0.812902	3.355856e-51	-8.615850e-47
Msa0030720	Msa0830180	0.804252	2.361308e-49	-8.615850e-47
Msa0030720	Msa0879950	0.984707	2.383442e-161	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030720	MtrunA17_Chr1g0188241	96.903	226	3	1	1	226	1	222	2.70e-163	449
Msa0030720	MtrunA17_Chr6g0476521	70.742	229	58	5	1	226	1	223	2.63e-109	313
Msa0030720	MtrunA17_Chr6g0476511	68.860	228	62	5	1	226	1	221	1.50e-106	306
Msa0030720	MtrunA17_Chr4g0011081	49.776	223	104	4	1	223	1	215	1.45e-66	204
Msa0030720	MtrunA17_Chr7g0275631	35.065	231	121	9	4	216	3	222	3.47e-28	106
Msa0030720	MtrunA17_Chr4g0056901	30.973	226	136	7	4	215	5	224	2.70e-23	93.6
Msa0030720	MtrunA17_Chr4g0011241	28.512	242	128	10	4	215	5	231	1.74e-20	86.3
Msa0030720	MtrunA17_Chr5g0398711	26.087	230	148	8	3	215	54	278	9.97e-19	82.4
Msa0030720	MtrunA17_Chr5g0398721	28.448	232	138	12	4	215	8	231	3.85e-17	77.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0030720	AT5G62280.1	54.894	235	93	7	1	224	1	233	3.58e-69	212
Msa0030720	AT2G45360.1	31.250	224	128	10	4	215	5	214	1.31e-24	97.1
Msa0030720	AT3G60780.1	28.761	226	134	10	4	215	5	217	2.88e-21	88.6
Msa0030720	AT1G12320.1	28.440	218	141	6	4	216	5	212	4.38e-14	68.9
Msa0030720	AT1G62840.1	29.060	234	129	10	4	215	5	223	4.81e-12	63.5

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Find 50 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAAGAAACTGAACTA+TGG	0.413679	1_1:+57137123	Msa0030720:exon
AAAGGATCTTGGTTATCATG+TGG	0.648675	1_1:+57137726	Msa0030720:CDS
AAATGGTGGAACAATTGTTG+TGG	0.637602	1_1:+57137683	Msa0030720:CDS
AATGGTGGAACAATTGTTGT+GGG	0.563879	1_1:+57137684	Msa0030720:CDS
ACAATTGTTGTGGGGTACAA+TGG	0.562830	1_1:+57137693	Msa0030720:CDS
ACCAAAAGCCCTTCTCCAAT+TGG	0.423738	1_1:-57137768	None:intergenic
AGCAATTCCTAAGGCATGTT+TGG	0.381874	1_1:-57137517	None:intergenic
AGCAGCAATTAAAGCTAGTG+TGG	0.570090	1_1:-57137433	None:intergenic
AGCTCAAGAAGTGCTTCTAT+TGG	0.369696	1_1:+57137569	Msa0030720:CDS
AGTGCTAAGATAGGCCTTTG+TGG	0.521249	1_1:-57137167	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCAAGAAACTGAACTA+TGG	+	chr1_1:57137123-57137142	Msa0030720:exon	30.0%
GCAATAAGATCTTTATGGTT+AGG	-	chr1_1:57137495-57137514	None:intergenic	30.0%
TGGATGCAATAAGATCTTTA+TGG	-	chr1_1:57137500-57137519	None:intergenic	30.0%
AAAAATCAGCTGCTTCATAT+TGG	-	chr1_1:57137596-57137615	None:intergenic	30.0%
GATTCTCAAAACAATTCAAG+AGG	+	chr1_1:57137650-57137669	Msa0030720:CDS	30.0%
GAGAATAATAGTCCAAGATA+TGG	+	chr1_1:57137810-57137829	Msa0030720:CDS	30.0%
ATATGGAACAAGTAGATCAA+TGG	+	chr1_1:57137827-57137846	Msa0030720:CDS	30.0%
TATGGAACAAGTAGATCAAT+GGG	+	chr1_1:57137828-57137847	Msa0030720:CDS	30.0%
AAAACCAAGAAACTGAACTA+TGG	+	chr1_1:57137123-57137142	Msa0030720:exon	30.0%
GCAATAAGATCTTTATGGTT+AGG	-	chr1_1:57137495-57137514	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	57137091	57137950	57137091	ID=Msa0030720;Name=Msa0030720
chr1_1	mRNA	57137091	57137950	57137091	ID=Msa0030720-mRNA-1;Parent=Msa0030720;Name=Msa0030720-mRNA-1;_AED=0.02;_eAED=0.02;_QI=51\|1\|0.5\|1\|1\|1\|2\|0\|226
chr1_1	exon	57137091	57137198	57137091	ID=Msa0030720-mRNA-1:exon:17191;Parent=Msa0030720-mRNA-1
chr1_1	exon	57137327	57137950	57137327	ID=Msa0030720-mRNA-1:exon:17192;Parent=Msa0030720-mRNA-1
chr1_1	five_prime_UTR	57137091	57137141	57137091	ID=Msa0030720-mRNA-1:five_prime_utr;Parent=Msa0030720-mRNA-1
chr1_1	CDS	57137142	57137198	57137142	ID=Msa0030720-mRNA-1:cds;Parent=Msa0030720-mRNA-1
chr1_1	CDS	57137327	57137950	57137327	ID=Msa0030720-mRNA-1:cds;Parent=Msa0030720-mRNA-1

Gene Sequence

>Msa0030720

ATGGCTTGTTGGTCTGCAGAAAATGCCACAAAGGCCTATCTTAGCACTTTGAAAATGGGTCAAAAAGCCAAAGAACCAAATGTAGCTGAATTTATATCAGCACTAGCTGCAGGAAACAATGCACAAATGATGATTGTAGCATATGCTAATGTAGCAGATTCTACCACACTAGCTTTAATTGCTGCTGCTAATCAAACTGGTGGACAAGTAATTTGTATTGTCCCTAACCATAAAGATCTTATTGCATCCAAACATGCCTTAGGAATTGCTTCACATCAAGTCCAATTCATGGTTGGAAAAGCTCAAGAAGTGCTTCTATTGGACCAATATGAAGCAGCTGATTTTTTGCTCATTGATTGTAATATTAAGAATCATGAAGAGATTCTCAAAACAATTCAAGAGGGTAGAAATGTAAATGGTGGAACAATTGTTGTGGGGTACAATGGATTTAGTTGCAAAGGATCTTGGTTATCATGTGGATCAAAGACTCAACTTTTGCCAATTGGAGAAGGGCTTTTGGTGACAAGATTTGGAATAAGTGAGAATAATAGTCCAAGATATGGAACAAGTAGATCAATGGGAAAGATTAAAAGTCGTTGGGTTGTTAAGGTTGATAAATGCACGGGTGAGGAACATGTGTTTAGGGTCAGATTTCCTCACGGGAAAGTTATTCAAGCTTAA

Protein sequence

>Msa0030720

MACWSAENATKAYLSTLKMGQKAKEPNVAEFISALAAGNNAQMMIVAYANVADSTTLALIAAANQTGGQVICIVPNHKDLIASKHALGIASHQVQFMVGKAQEVLLLDQYEAADFLLIDCNIKNHEEILKTIQEGRNVNGGTIVVGYNGFSCKGSWLSCGSKTQLLPIGEGLLVTRFGISENNSPRYGTSRSMGKIKSRWVVKVDKCTGEEHVFRVRFPHGKVIQA*