AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0045640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045640 MtrunA17_Chr1g0212831 93.000 100 5 1 1 98 1 100 1.92e-61 182
Msa0045640 MtrunA17_Chr7g0222601 76.087 92 18 3 10 101 15 102 2.87e-42 134
Msa0045640 MtrunA17_Chr7g0263161 59.140 93 28 3 6 96 4 88 5.84e-31 105
Msa0045640 MtrunA17_Chr8g0368511 57.143 56 24 0 9 64 48 103 4.50e-20 78.2
Msa0045640 MtrunA17_Chr1g0171941 45.614 57 31 0 11 67 14 70 7.77e-12 59.7
Msa0045640 MtrunA17_Chr8g0347131 43.860 57 32 0 11 67 55 111 2.66e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0045640 AT3G55240.1 69.792 96 24 3 1 96 1 91 5.93e-41 130
Msa0045640 AT3G28990.1 65.116 86 28 1 5 90 4 87 1.92e-32 108
Msa0045640 AT5G02580.1 56.989 93 35 2 1 92 1 89 2.98e-32 108
Msa0045640 AT5G02580.3 53.165 79 33 1 14 92 8 82 1.61e-24 89.0
Msa0045640 AT5G02580.2 75.510 49 11 1 1 48 1 49 6.24e-20 76.6
Msa0045640 AT1G10657.2 52.632 57 27 0 9 65 22 78 1.51e-19 76.6
Msa0045640 AT1G10657.3 52.632 57 27 0 9 65 22 78 1.85e-19 76.6
Msa0045640 AT1G10657.1 37.037 81 27 1 9 65 22 102 5.60e-14 63.2
Msa0045640 AT1G10657.4 37.037 81 27 1 9 65 22 102 7.48e-14 62.8
Msa0045640 AT2G46420.3 43.860 57 32 0 11 67 52 108 6.24e-12 60.5
Msa0045640 AT2G46420.1 43.860 57 32 0 11 67 52 108 6.24e-12 60.5
Msa0045640 AT2G46420.2 43.860 57 32 0 11 67 52 108 6.68e-12 60.5
Msa0045640 AT5G04090.6 43.860 57 32 0 11 67 31 87 7.04e-12 60.5
Msa0045640 AT5G04090.2 43.860 57 32 0 11 67 14 70 7.95e-12 60.1
Msa0045640 AT3G10250.2 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa0045640 AT3G10250.5 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa0045640 AT3G10250.1 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa0045640 AT3G10250.4 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa0045640 AT3G10250.3 43.860 57 32 0 11 67 14 70 1.64e-11 59.3

Find 32 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATATATATGACCAATTT+AGG 0.171764 1_1:+77599369 Msa0045640:three_prime_UTR
TTTCAAGAGTTGATTGATTA+TGG 0.214655 1_1:-77599346 None:intergenic
ATACCAGTGGATGTTATAAT+TGG 0.219027 1_1:-77597635 None:intergenic
CTCTTTGGCTTCATGTTATA+TGG 0.220781 1_1:-77599221 None:intergenic
ACATCTGAGTTGTCTCTTCC+TGG 0.233068 1_1:-77599265 None:intergenic
TGGATTTATATTTGCATGTT+TGG 0.252690 1_1:-77597615 None:intergenic
GTTGCTCCTCTACATTATTA+TGG 0.253456 1_1:+77599501 Msa0045640:three_prime_UTR
CGCTTCCATGCATTCTTCCT+TGG 0.321815 1_1:-77597588 None:intergenic
TGCATGGTAAGTACCTAAAT+TGG 0.364202 1_1:-77599382 None:intergenic
TGACCCTTGTATATATTTGA+TGG 0.370068 1_1:-77597284 None:intergenic
GTCGTGTTGTGCACCTTTAG+AGG 0.436240 1_1:-77599315 None:intergenic
AAAGAAGATGATCTCAGCTT+CGG 0.465702 1_1:+77599289 Msa0045640:CDS
TCAGCTTCGGATTCCTCTAA+AGG 0.506755 1_1:+77599302 Msa0045640:CDS
TTCGGCTTCATACATTCACT+TGG 0.510731 1_1:+77597397 Msa0045640:CDS
CATCTGAGTTGTCTCTTCCT+GGG 0.513019 1_1:-77599264 None:intergenic
TGCAATACCAAGAGCTAGCA+TGG 0.513676 1_1:+77597361 Msa0045640:exon
TGAGTTAGAGAAGGTGAACA+AGG 0.535925 1_1:+77599187 Msa0045640:CDS
AATCCAATTATAACATCCAC+TGG 0.539693 1_1:+77597632 Msa0045640:CDS
AAACATATTTATCAAGAACT+AGG 0.557315 1_1:+77597309 Msa0045640:five_prime_UTR
AGTTCCATCAAATATATACA+AGG 0.559883 1_1:+77597280 Msa0045640:five_prime_UTR
CATGACCAAGGAAGAATGCA+TGG 0.570865 1_1:+77597583 Msa0045640:CDS
TGAAGAGAAAATGATGTCCC+AGG 0.581534 1_1:+77599247 Msa0045640:CDS
GCAATACCAAGAGCTAGCAT+GGG 0.596506 1_1:+77597362 Msa0045640:exon
GCGCATAGACTACTAGCACT+AGG 0.603360 1_1:-77599457 None:intergenic
ACAATACCATAATAATGTAG+AGG 0.622036 1_1:-77599507 None:intergenic
AGTGTGGAATGAGTTAGAGA+AGG 0.642407 1_1:+77599178 Msa0045640:intron
GAATATTATATATACTACAA+AGG 0.655145 1_1:-77599563 None:intergenic
TTTGATCTTTCACATGACCA+AGG 0.655387 1_1:+77597571 Msa0045640:CDS
GTTCCATCAAATATATACAA+GGG 0.664258 1_1:+77597281 Msa0045640:five_prime_UTR
CAATACCAAGAGCTAGCATG+GGG 0.665262 1_1:+77597363 Msa0045640:exon
ATATACTACAAAGGTTGACA+AGG 0.676891 1_1:-77599554 None:intergenic
AATACCAAGAGCTAGCATGG+GGG 0.797315 1_1:+77597364 Msa0045640:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATCAAATCTT+AGG + chr1_1:77598323-77598342 Msa0045640:intron 10.0%
!! TATAATAATATGTTTACATA+CGG + chr1_1:77598604-77598623 Msa0045640:intron 10.0%
!!! AAAACACAATTTTTTTTTTT+CGG - chr1_1:77598880-77598899 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG + chr1_1:77598954-77598973 Msa0045640:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG + chr1_1:77598955-77598974 Msa0045640:intron 10.0%
!! AAAAAAAAAAATCAAATCTT+AGG + chr1_1:77598323-77598342 Msa0045640:intron 10.0%
!! TATAATAATATGTTTACATA+CGG + chr1_1:77598604-77598623 Msa0045640:intron 10.0%
!!! AAAACACAATTTTTTTTTTT+CGG - chr1_1:77598880-77598899 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG + chr1_1:77598954-77598973 Msa0045640:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG + chr1_1:77598955-77598974 Msa0045640:intron 10.0%
!!! ATCTACTATTTTACTAAAAA+TGG + chr1_1:77597866-77597885 Msa0045640:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG + chr1_1:77598361-77598380 Msa0045640:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG + chr1_1:77598605-77598624 Msa0045640:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG + chr1_1:77598982-77599001 Msa0045640:intron 15.0%
!! ATTATATATATGACCAATTT+AGG + chr1_1:77599369-77599388 Msa0045640:three_prime_UTR 15.0%
!!! GTTTTGATAATAATATAGTA+GGG - chr1_1:77599438-77599457 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG - chr1_1:77599566-77599585 None:intergenic 15.0%
!!! ATCTACTATTTTACTAAAAA+TGG + chr1_1:77597866-77597885 Msa0045640:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG + chr1_1:77598361-77598380 Msa0045640:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG + chr1_1:77598605-77598624 Msa0045640:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG + chr1_1:77598982-77599001 Msa0045640:intron 15.0%
!! ATTATATATATGACCAATTT+AGG + chr1_1:77599369-77599388 Msa0045640:three_prime_UTR 15.0%
!!! GTTTTGATAATAATATAGTA+GGG - chr1_1:77599438-77599457 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG - chr1_1:77599566-77599585 None:intergenic 15.0%
!! AAACATATTTATCAAGAACT+AGG + chr1_1:77597309-77597328 Msa0045640:five_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG + chr1_1:77597526-77597545 Msa0045640:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG + chr1_1:77597684-77597703 Msa0045640:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG - chr1_1:77597820-77597839 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG - chr1_1:77598002-77598021 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG + chr1_1:77598030-77598049 Msa0045640:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG - chr1_1:77598132-77598151 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG - chr1_1:77599439-77599458 None:intergenic 20.0%
!! AAACATATTTATCAAGAACT+AGG + chr1_1:77597309-77597328 Msa0045640:five_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG + chr1_1:77597526-77597545 Msa0045640:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG + chr1_1:77597684-77597703 Msa0045640:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG - chr1_1:77597820-77597839 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG - chr1_1:77598002-77598021 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG + chr1_1:77598030-77598049 Msa0045640:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG - chr1_1:77598132-77598151 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG - chr1_1:77599439-77599458 None:intergenic 20.0%
! AGTTCCATCAAATATATACA+AGG + chr1_1:77597280-77597299 Msa0045640:five_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG + chr1_1:77597281-77597300 Msa0045640:five_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG - chr1_1:77597500-77597519 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG - chr1_1:77597618-77597637 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG - chr1_1:77597651-77597670 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG + chr1_1:77597897-77597916 Msa0045640:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG + chr1_1:77598029-77598048 Msa0045640:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG + chr1_1:77598031-77598050 Msa0045640:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG + chr1_1:77598032-77598051 Msa0045640:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG - chr1_1:77598088-77598107 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG + chr1_1:77598389-77598408 Msa0045640:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG - chr1_1:77598523-77598542 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG - chr1_1:77598538-77598557 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG + chr1_1:77598740-77598759 Msa0045640:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG - chr1_1:77598833-77598852 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG + chr1_1:77599110-77599129 Msa0045640:intron 25.0%
! ATGAAATAATGAATGTAGTG+TGG + chr1_1:77599162-77599181 Msa0045640:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG - chr1_1:77599349-77599368 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG - chr1_1:77599510-77599529 None:intergenic 25.0%
! AGTTCCATCAAATATATACA+AGG + chr1_1:77597280-77597299 Msa0045640:five_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG + chr1_1:77597281-77597300 Msa0045640:five_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG - chr1_1:77597500-77597519 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG - chr1_1:77597618-77597637 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG - chr1_1:77597651-77597670 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG + chr1_1:77597897-77597916 Msa0045640:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG + chr1_1:77598029-77598048 Msa0045640:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG + chr1_1:77598031-77598050 Msa0045640:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG + chr1_1:77598032-77598051 Msa0045640:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG - chr1_1:77598088-77598107 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG + chr1_1:77598389-77598408 Msa0045640:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG - chr1_1:77598523-77598542 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG - chr1_1:77598538-77598557 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG + chr1_1:77598740-77598759 Msa0045640:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG - chr1_1:77598833-77598852 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG + chr1_1:77599110-77599129 Msa0045640:intron 25.0%
! ATGAAATAATGAATGTAGTG+TGG + chr1_1:77599162-77599181 Msa0045640:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG - chr1_1:77599349-77599368 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG - chr1_1:77599510-77599529 None:intergenic 25.0%
TGACCCTTGTATATATTTGA+TGG - chr1_1:77597287-77597306 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG + chr1_1:77597632-77597651 Msa0045640:CDS 30.0%
ATACCAGTGGATGTTATAAT+TGG - chr1_1:77597638-77597657 None:intergenic 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG + chr1_1:77598291-77598310 Msa0045640:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG + chr1_1:77598403-77598422 Msa0045640:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG - chr1_1:77598834-77598853 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG + chr1_1:77598844-77598863 Msa0045640:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG + chr1_1:77599048-77599067 Msa0045640:intron 30.0%
CTCATGAACTGAAATTACAT+TGG + chr1_1:77599109-77599128 Msa0045640:intron 30.0%
! ATTTTCTCTTCACTACTCTT+TGG - chr1_1:77599239-77599258 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG - chr1_1:77599413-77599432 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG - chr1_1:77599557-77599576 None:intergenic 30.0%
TGACCCTTGTATATATTTGA+TGG - chr1_1:77597287-77597306 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG + chr1_1:77597632-77597651 Msa0045640:CDS 30.0%
ATACCAGTGGATGTTATAAT+TGG - chr1_1:77597638-77597657 None:intergenic 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG + chr1_1:77598291-77598310 Msa0045640:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG + chr1_1:77598403-77598422 Msa0045640:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG - chr1_1:77598834-77598853 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG + chr1_1:77598844-77598863 Msa0045640:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG + chr1_1:77599048-77599067 Msa0045640:intron 30.0%
CTCATGAACTGAAATTACAT+TGG + chr1_1:77599109-77599128 Msa0045640:intron 30.0%
! ATTTTCTCTTCACTACTCTT+TGG - chr1_1:77599239-77599258 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG - chr1_1:77599413-77599432 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG - chr1_1:77599557-77599576 None:intergenic 30.0%
! AGATCAAACACTTTTCGATC+AGG - chr1_1:77597559-77597578 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG + chr1_1:77597571-77597590 Msa0045640:CDS 35.0%
CCAATTGGTTAACAATCAAG+TGG - chr1_1:77597939-77597958 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG + chr1_1:77597936-77597955 Msa0045640:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG + chr1_1:77597944-77597963 Msa0045640:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG + chr1_1:77597945-77597964 Msa0045640:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG - chr1_1:77598126-77598145 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG + chr1_1:77598193-77598212 Msa0045640:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG - chr1_1:77598226-77598245 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG - chr1_1:77598227-77598246 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG + chr1_1:77598224-77598243 Msa0045640:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG - chr1_1:77598228-77598247 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG + chr1_1:77598848-77598867 Msa0045640:intron 35.0%
TAGATCTACTCATCTAGACA+AGG - chr1_1:77598904-77598923 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG + chr1_1:77598906-77598925 Msa0045640:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG + chr1_1:77598999-77599018 Msa0045640:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG - chr1_1:77599224-77599243 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG + chr1_1:77599289-77599308 Msa0045640:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG - chr1_1:77599385-77599404 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG - chr1_1:77599409-77599428 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG + chr1_1:77599407-77599426 Msa0045640:three_prime_UTR 35.0%
GTTGCTCCTCTACATTATTA+TGG + chr1_1:77599501-77599520 Msa0045640:three_prime_UTR 35.0%
! AGATCAAACACTTTTCGATC+AGG - chr1_1:77597559-77597578 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG + chr1_1:77597571-77597590 Msa0045640:CDS 35.0%
CCAATTGGTTAACAATCAAG+TGG - chr1_1:77597939-77597958 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG + chr1_1:77597936-77597955 Msa0045640:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG + chr1_1:77597944-77597963 Msa0045640:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG + chr1_1:77597945-77597964 Msa0045640:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG - chr1_1:77598126-77598145 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG + chr1_1:77598193-77598212 Msa0045640:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG - chr1_1:77598226-77598245 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG - chr1_1:77598227-77598246 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG + chr1_1:77598224-77598243 Msa0045640:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG - chr1_1:77598228-77598247 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG + chr1_1:77598848-77598867 Msa0045640:intron 35.0%
TAGATCTACTCATCTAGACA+AGG - chr1_1:77598904-77598923 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG + chr1_1:77598906-77598925 Msa0045640:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG + chr1_1:77598999-77599018 Msa0045640:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG - chr1_1:77599224-77599243 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG + chr1_1:77599289-77599308 Msa0045640:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG - chr1_1:77599385-77599404 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG - chr1_1:77599409-77599428 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG + chr1_1:77599407-77599426 Msa0045640:three_prime_UTR 35.0%
GTTGCTCCTCTACATTATTA+TGG + chr1_1:77599501-77599520 Msa0045640:three_prime_UTR 35.0%
TTCGGCTTCATACATTCACT+TGG + chr1_1:77597397-77597416 Msa0045640:CDS 40.0%
! CATGTCTAGAGCTAACTTAG+AGG + chr1_1:77598149-77598168 Msa0045640:intron 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG - chr1_1:77598215-77598234 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG + chr1_1:77598223-77598242 Msa0045640:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG + chr1_1:77598245-77598264 Msa0045640:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG + chr1_1:77598246-77598265 Msa0045640:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG + chr1_1:77598256-77598275 Msa0045640:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG + chr1_1:77598280-77598299 Msa0045640:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG - chr1_1:77598855-77598874 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG + chr1_1:77598907-77598926 Msa0045640:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG + chr1_1:77599021-77599040 Msa0045640:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG + chr1_1:77599178-77599197 Msa0045640:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG + chr1_1:77599187-77599206 Msa0045640:CDS 40.0%
TGAAGAGAAAATGATGTCCC+AGG + chr1_1:77599247-77599266 Msa0045640:CDS 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG - chr1_1:77599401-77599420 None:intergenic 40.0%
TTCGGCTTCATACATTCACT+TGG + chr1_1:77597397-77597416 Msa0045640:CDS 40.0%
! CATGTCTAGAGCTAACTTAG+AGG + chr1_1:77598149-77598168 Msa0045640:intron 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG - chr1_1:77598215-77598234 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG + chr1_1:77598223-77598242 Msa0045640:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG + chr1_1:77598245-77598264 Msa0045640:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG + chr1_1:77598246-77598265 Msa0045640:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG + chr1_1:77598256-77598275 Msa0045640:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG + chr1_1:77598280-77598299 Msa0045640:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG - chr1_1:77598855-77598874 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG + chr1_1:77598907-77598926 Msa0045640:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG + chr1_1:77599021-77599040 Msa0045640:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG + chr1_1:77599178-77599197 Msa0045640:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG + chr1_1:77599187-77599206 Msa0045640:CDS 40.0%
TGAAGAGAAAATGATGTCCC+AGG + chr1_1:77599247-77599266 Msa0045640:CDS 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG - chr1_1:77599401-77599420 None:intergenic 40.0%
TGCAATACCAAGAGCTAGCA+TGG + chr1_1:77597361-77597380 Msa0045640:exon 45.0%
GCAATACCAAGAGCTAGCAT+GGG + chr1_1:77597362-77597381 Msa0045640:exon 45.0%
CAATACCAAGAGCTAGCATG+GGG + chr1_1:77597363-77597382 Msa0045640:exon 45.0%
AATACCAAGAGCTAGCATGG+GGG + chr1_1:77597364-77597383 Msa0045640:exon 45.0%
CATGACCAAGGAAGAATGCA+TGG + chr1_1:77597583-77597602 Msa0045640:CDS 45.0%
GCGTGCATGCTTAAGAAGAT+AGG + chr1_1:77597972-77597991 Msa0045640:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG + chr1_1:77598250-77598269 Msa0045640:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG + chr1_1:77598912-77598931 Msa0045640:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG - chr1_1:77599267-77599286 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG - chr1_1:77599268-77599287 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG + chr1_1:77599302-77599321 Msa0045640:CDS 45.0%
TGCAATACCAAGAGCTAGCA+TGG + chr1_1:77597361-77597380 Msa0045640:exon 45.0%
GCAATACCAAGAGCTAGCAT+GGG + chr1_1:77597362-77597381 Msa0045640:exon 45.0%
CAATACCAAGAGCTAGCATG+GGG + chr1_1:77597363-77597382 Msa0045640:exon 45.0%
AATACCAAGAGCTAGCATGG+GGG + chr1_1:77597364-77597383 Msa0045640:exon 45.0%
CATGACCAAGGAAGAATGCA+TGG + chr1_1:77597583-77597602 Msa0045640:CDS 45.0%
GCGTGCATGCTTAAGAAGAT+AGG + chr1_1:77597972-77597991 Msa0045640:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG + chr1_1:77598250-77598269 Msa0045640:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG + chr1_1:77598912-77598931 Msa0045640:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG - chr1_1:77599267-77599286 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG - chr1_1:77599268-77599287 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG + chr1_1:77599302-77599321 Msa0045640:CDS 45.0%
CGCTTCCATGCATTCTTCCT+TGG - chr1_1:77597591-77597610 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG - chr1_1:77597954-77597973 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG + chr1_1:77599003-77599022 Msa0045640:intron 50.0%
CACACACGCATGCTAGTGTT+TGG - chr1_1:77599082-77599101 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG - chr1_1:77599318-77599337 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG - chr1_1:77599460-77599479 None:intergenic 50.0%
CGCTTCCATGCATTCTTCCT+TGG - chr1_1:77597591-77597610 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG - chr1_1:77597954-77597973 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG + chr1_1:77599003-77599022 Msa0045640:intron 50.0%
CACACACGCATGCTAGTGTT+TGG - chr1_1:77599082-77599101 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG - chr1_1:77599318-77599337 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG - chr1_1:77599460-77599479 None:intergenic 50.0%
CATGGGGGACTCGTCTTCTT+CGG + chr1_1:77597379-77597398 Msa0045640:exon 55.0%
AGGAATAGGCTGCGCGATCT+AGG + chr1_1:77598270-77598289 Msa0045640:intron 55.0%
CATGGGGGACTCGTCTTCTT+CGG + chr1_1:77597379-77597398 Msa0045640:exon 55.0%
AGGAATAGGCTGCGCGATCT+AGG + chr1_1:77598270-77598289 Msa0045640:intron 55.0%
! GAGTCCCCCATGCTAGCTCT+TGG - chr1_1:77597371-77597390 None:intergenic 60.0%
! GAGTCCCCCATGCTAGCTCT+TGG - chr1_1:77597371-77597390 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 77597277 77599614 77597277 ID=Msa0045640;Name=Msa0045640
chr1_1 mRNA 77597277 77599614 77597277 ID=Msa0045640-mRNA-1;Parent=Msa0045640;Name=Msa0045640-mRNA-1;_AED=0.09;_eAED=0.09;_QI=103|1|1|1|0|0|3|271|102
chr1_1 exon 77597277 77597418 77597277 ID=Msa0045640-mRNA-1:exon:26564;Parent=Msa0045640-mRNA-1
chr1_1 exon 77597548 77597653 77597548 ID=Msa0045640-mRNA-1:exon:26565;Parent=Msa0045640-mRNA-1
chr1_1 exon 77599180 77599614 77599180 ID=Msa0045640-mRNA-1:exon:26566;Parent=Msa0045640-mRNA-1
chr1_1 five_prime_UTR 77597277 77597379 77597277 ID=Msa0045640-mRNA-1:five_prime_utr;Parent=Msa0045640-mRNA-1
chr1_1 CDS 77597380 77597418 77597380 ID=Msa0045640-mRNA-1:cds;Parent=Msa0045640-mRNA-1
chr1_1 CDS 77597548 77597653 77597548 ID=Msa0045640-mRNA-1:cds;Parent=Msa0045640-mRNA-1
chr1_1 CDS 77599180 77599343 77599180 ID=Msa0045640-mRNA-1:cds;Parent=Msa0045640-mRNA-1
chr1_1 three_prime_UTR 77599344 77599614 77599344 ID=Msa0045640-mRNA-1:three_prime_utr;Parent=Msa0045640-mRNA-1
Gene Sequence

>Msa0045640

ATGGGGGACTCGTCTTCTTCGGCTTCATACATTCACTTGGTGCAGCACCTGATCGAAAAGTGTTTGATCTTTCACATGACCAAGGAAGAATGCATGGAAGCGCTCTCCAAACATGCAAATATAAATCCAATTATAACATCCACTGTGTGGAATGAGTTAGAGAAGGTGAACAAGGAATTTTTTGAGCCATATAACATGAAGCCAAAGAGTAGTGAAGAGAAAATGATGTCCCAGGAAGAGACAACTCAGATGTTAAAGAAGATGATCTCAGCTTCGGATTCCTCTAAAGGTGCACAACACGACGACTAA
Protein sequence

>Msa0045640

MGDSSSSASYIHLVQHLIEKCLIFHMTKEECMEALSKHANINPIITSTVWNELEKVNKEFFEPYNMKPKSSEEKMMSQEETTQMLKKMISASDSSKGAQHDD*