AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0623290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623290 MtrunA17_Chr1g0212831 93.000 100 5 1 12 109 1 100 1.51e-61 183
Msa0623290 MtrunA17_Chr7g0222601 76.087 92 18 3 21 112 15 102 2.10e-42 134
Msa0623290 MtrunA17_Chr7g0263161 59.140 93 28 3 17 107 4 88 9.25e-31 104
Msa0623290 MtrunA17_Chr8g0368511 57.143 56 24 0 20 75 48 103 7.40e-20 78.2
Msa0623290 MtrunA17_Chr1g0171941 45.614 57 31 0 22 78 14 70 1.16e-11 59.7
Msa0623290 MtrunA17_Chr8g0347131 43.860 57 32 0 22 78 55 111 5.14e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623290 AT3G55240.1 69.792 96 24 3 12 107 1 91 5.17e-41 131
Msa0623290 AT3G28990.1 65.116 86 28 1 16 101 4 87 1.85e-32 109
Msa0623290 AT5G02580.1 56.989 93 35 2 12 103 1 89 5.00e-32 108
Msa0623290 AT5G02580.3 53.165 79 33 1 25 103 8 82 2.72e-24 88.6
Msa0623290 AT5G02580.2 75.510 49 11 1 12 59 1 49 6.13e-20 77.0
Msa0623290 AT1G10657.2 52.632 57 27 0 20 76 22 78 2.86e-19 76.6
Msa0623290 AT1G10657.3 52.632 57 27 0 20 76 22 78 3.70e-19 76.3
Msa0623290 AT1G10657.1 37.037 81 27 1 20 76 22 102 1.02e-13 62.8
Msa0623290 AT1G10657.4 37.037 81 27 1 20 76 22 102 1.35e-13 62.8
Msa0623290 AT5G04090.6 43.860 57 32 0 22 78 31 87 1.11e-11 60.1
Msa0623290 AT5G04090.2 43.860 57 32 0 22 78 14 70 1.28e-11 60.1
Msa0623290 AT2G46420.2 43.860 57 32 0 22 78 52 108 1.44e-11 60.1
Msa0623290 AT2G46420.3 43.860 57 32 0 22 78 52 108 1.57e-11 59.7
Msa0623290 AT2G46420.1 43.860 57 32 0 22 78 52 108 1.57e-11 59.7
Msa0623290 AT3G10250.2 43.860 57 32 0 22 78 14 70 1.70e-11 59.7
Msa0623290 AT3G10250.5 43.860 57 32 0 22 78 14 70 1.70e-11 59.7
Msa0623290 AT3G10250.1 43.860 57 32 0 22 78 14 70 1.70e-11 59.7
Msa0623290 AT3G10250.4 43.860 57 32 0 22 78 14 70 1.70e-11 59.7
Msa0623290 AT3G10250.3 43.860 57 32 0 22 78 14 70 2.61e-11 58.9
Msa0623290 AT3G61700.2 42.105 57 33 0 22 78 55 111 5.95e-11 58.2
Msa0623290 AT3G61700.1 42.105 57 33 0 22 78 55 111 6.15e-11 58.2
Msa0623290 AT3G61700.3 42.105 57 33 0 22 78 55 111 6.15e-11 58.2
Msa0623290 AT3G61700.4 42.105 57 33 0 22 78 53 109 6.77e-11 58.2

Find 28 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATATATATGACCAATTT+AGG 0.171764 4_3:-470013 Msa0623290:three_prime_UTR
TTTCAAGAGTTGATTGATTA+TGG 0.214655 4_3:+470036 None:intergenic
ATACCAGTGGATGTTATAAT+TGG 0.219027 4_3:+471752 None:intergenic
CTCTTTGGCTTCATGTTATA+TGG 0.220781 4_3:+470161 None:intergenic
ACATCTGAGTTGTCTCTTCC+TGG 0.233068 4_3:+470117 None:intergenic
TGGATTTATATTTGCATGTT+TGG 0.252690 4_3:+471772 None:intergenic
GTTGCTCCTCTACATTATTA+TGG 0.253456 4_3:-469881 Msa0623290:three_prime_UTR
CGCTTCCATGCATTCTTCCT+TGG 0.321815 4_3:+471799 None:intergenic
TGCATGGTAAGTACCTAAAT+TGG 0.364202 4_3:+470000 None:intergenic
GTCGTGTTGTGCACCTTTAG+AGG 0.436240 4_3:+470067 None:intergenic
AAAGAAGATGATCTCAGCTT+CGG 0.465702 4_3:-470093 Msa0623290:CDS
TCAGCTTCGGATTCCTCTAA+AGG 0.506755 4_3:-470080 Msa0623290:CDS
TTCGGCTTCATACATTCACT+TGG 0.510731 4_3:-471990 Msa0623290:intron
CATCTGAGTTGTCTCTTCCT+GGG 0.513019 4_3:+470118 None:intergenic
TGCAATACCAAGAGCTAGCA+TGG 0.513676 4_3:-472026 Msa0623290:CDS
TGAGTTAGAGAAGGTGAACA+AGG 0.535925 4_3:-470195 Msa0623290:CDS
AATCCAATTATAACATCCAC+TGG 0.539693 4_3:-471755 Msa0623290:intron
CATGACCAAGGAAGAATGCA+TGG 0.570865 4_3:-471804 Msa0623290:CDS
TGAAGAGAAAATGATGTCCC+AGG 0.581534 4_3:-470135 Msa0623290:CDS
GCAATACCAAGAGCTAGCAT+GGG 0.596506 4_3:-472025 Msa0623290:CDS
GCGCATAGACTACTAGCACT+AGG 0.603360 4_3:+469925 None:intergenic
ACAATACCATAATAATGTAG+AGG 0.622036 4_3:+469875 None:intergenic
AGTGTGGAATGAGTTAGAGA+AGG 0.642407 4_3:-470204 Msa0623290:CDS
GAATATTATATATACTACAA+AGG 0.655145 4_3:+469819 None:intergenic
TTTGATCTTTCACATGACCA+AGG 0.655387 4_3:-471816 Msa0623290:CDS
CAATACCAAGAGCTAGCATG+GGG 0.665262 4_3:-472024 Msa0623290:CDS
ATATACTACAAAGGTTGACA+AGG 0.676891 4_3:+469828 None:intergenic
AATACCAAGAGCTAGCATGG+GGG 0.797315 4_3:-472023 Msa0623290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATCAAATCTT+AGG - chr4_3:471027-471046 Msa0623290:intron 10.0%
!! TATAATAATATGTTTACATA+CGG - chr4_3:471308-471327 Msa0623290:intron 10.0%
!!! AAAAACACAATTTTTTTTTT+CGG + chr4_3:471586-471605 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG - chr4_3:471659-471678 Msa0623290:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG - chr4_3:471660-471679 Msa0623290:intron 10.0%
!!! ATCTACTATTTTACTAAAAA+TGG - chr4_3:470566-470585 Msa0623290:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG - chr4_3:471065-471084 Msa0623290:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG - chr4_3:471309-471328 Msa0623290:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG - chr4_3:471687-471706 Msa0623290:intron 15.0%
!! ATTATATATATGACCAATTT+AGG - chr4_3:472074-472093 Msa0623290:intron 15.0%
!!! GTTTTGATAATAATATAGTA+GGG + chr4_3:472143-472162 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG + chr4_3:472271-472290 None:intergenic 15.0%
!! AAACATATTTATCAAGAACT+AGG - chr4_3:470009-470028 Msa0623290:three_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG - chr4_3:470226-470245 Msa0623290:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG - chr4_3:470384-470403 Msa0623290:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG + chr4_3:470520-470539 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG + chr4_3:470702-470721 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG - chr4_3:470730-470749 Msa0623290:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG + chr4_3:470832-470851 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG + chr4_3:472144-472163 None:intergenic 20.0%
! AAGAATGTTATAAAGTGTGA+CGG + chr4_3:469914-469933 None:intergenic 25.0%
! AGTTCCATCAAATATATACA+AGG - chr4_3:469980-469999 Msa0623290:three_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG - chr4_3:469981-470000 Msa0623290:three_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG + chr4_3:470200-470219 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG + chr4_3:470318-470337 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG + chr4_3:470351-470370 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG - chr4_3:470597-470616 Msa0623290:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG - chr4_3:470729-470748 Msa0623290:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG - chr4_3:470731-470750 Msa0623290:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG - chr4_3:470732-470751 Msa0623290:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG + chr4_3:470788-470807 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG - chr4_3:471093-471112 Msa0623290:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG + chr4_3:471227-471246 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG + chr4_3:471242-471261 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG - chr4_3:471444-471463 Msa0623290:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG + chr4_3:471539-471558 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG - chr4_3:471815-471834 Msa0623290:CDS 25.0%
! ATGAAATAATGAATGTAGTG+TGG - chr4_3:471867-471886 Msa0623290:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG + chr4_3:472054-472073 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG + chr4_3:472215-472234 None:intergenic 25.0%
GTACAAGACAAACAAATGAA+CGG - chr4_3:469798-469817 Msa0623290:three_prime_UTR 30.0%
! CTCTAAATCTAATCCTTTTC+TGG - chr4_3:469835-469854 Msa0623290:three_prime_UTR 30.0%
! GCTGATAAATGCTATTTGAT+TGG + chr4_3:469861-469880 None:intergenic 30.0%
AGAATGTTATAAAGTGTGAC+GGG + chr4_3:469913-469932 None:intergenic 30.0%
TGACCCTTGTATATATTTGA+TGG + chr4_3:469987-470006 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG - chr4_3:470332-470351 Msa0623290:intron 30.0%
ATACCAGTGGATGTTATAAT+TGG + chr4_3:470338-470357 None:intergenic 30.0%
! TCTAGAGCTAACTTAATTAG+AGG - chr4_3:470853-470872 Msa0623290:intron 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG - chr4_3:470995-471014 Msa0623290:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG - chr4_3:471107-471126 Msa0623290:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG + chr4_3:471540-471559 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG - chr4_3:471550-471569 Msa0623290:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG - chr4_3:471753-471772 Msa0623290:intron 30.0%
CTCATGAACTGAAATTACAT+TGG - chr4_3:471814-471833 Msa0623290:CDS 30.0%
! ATTTTCTCTTCACTACTCTT+TGG + chr4_3:471944-471963 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG + chr4_3:472118-472137 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG + chr4_3:472262-472281 None:intergenic 30.0%
GAATGTTATAAAGTGTGACG+GGG + chr4_3:469912-469931 None:intergenic 35.0%
! AGATCAAACACTTTTCGATC+AGG + chr4_3:470259-470278 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG - chr4_3:470271-470290 Msa0623290:intron 35.0%
CCAATTGGTTAACAATCAAG+TGG + chr4_3:470639-470658 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG - chr4_3:470636-470655 Msa0623290:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG - chr4_3:470644-470663 Msa0623290:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG - chr4_3:470645-470664 Msa0623290:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG + chr4_3:470826-470845 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG - chr4_3:470897-470916 Msa0623290:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG + chr4_3:470930-470949 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG + chr4_3:470931-470950 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG - chr4_3:470928-470947 Msa0623290:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG + chr4_3:470932-470951 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG - chr4_3:471554-471573 Msa0623290:intron 35.0%
TAGATCTACTCATCTAGACA+AGG + chr4_3:471609-471628 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG - chr4_3:471611-471630 Msa0623290:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG - chr4_3:471704-471723 Msa0623290:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG + chr4_3:471929-471948 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG - chr4_3:471994-472013 Msa0623290:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG + chr4_3:472090-472109 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG + chr4_3:472114-472133 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG - chr4_3:472112-472131 Msa0623290:intron 35.0%
GTTGCTCCTCTACATTATTA+TGG - chr4_3:472206-472225 Msa0623290:intron 35.0%
! GCTATTTGATTGGCCAGAAA+AGG + chr4_3:469851-469870 None:intergenic 40.0%
AATAGCATTTATCAGCCACG+TGG - chr4_3:469865-469884 Msa0623290:three_prime_UTR 40.0%
TTCGGCTTCATACATTCACT+TGG - chr4_3:470097-470116 Msa0623290:CDS 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG + chr4_3:470919-470938 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG - chr4_3:470927-470946 Msa0623290:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG - chr4_3:470949-470968 Msa0623290:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG - chr4_3:470950-470969 Msa0623290:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG - chr4_3:470960-470979 Msa0623290:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG - chr4_3:470984-471003 Msa0623290:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG + chr4_3:471561-471580 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG - chr4_3:471612-471631 Msa0623290:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG - chr4_3:471726-471745 Msa0623290:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG - chr4_3:471883-471902 Msa0623290:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG - chr4_3:471892-471911 Msa0623290:intron 40.0%
TGAAGAGAAAATGATGTCCC+AGG - chr4_3:471952-471971 Msa0623290:intron 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG + chr4_3:472106-472125 None:intergenic 40.0%
! TGTTATAAAGTGTGACGGGG+TGG + chr4_3:469909-469928 None:intergenic 45.0%
TGCAATACCAAGAGCTAGCA+TGG - chr4_3:470061-470080 Msa0623290:CDS 45.0%
GCAATACCAAGAGCTAGCAT+GGG - chr4_3:470062-470081 Msa0623290:CDS 45.0%
CAATACCAAGAGCTAGCATG+GGG - chr4_3:470063-470082 Msa0623290:CDS 45.0%
AATACCAAGAGCTAGCATGG+GGG - chr4_3:470064-470083 Msa0623290:CDS 45.0%
CATGACCAAGGAAGAATGCA+TGG - chr4_3:470283-470302 Msa0623290:intron 45.0%
GCGTGCATGCTTAAGAAGAT+AGG - chr4_3:470672-470691 Msa0623290:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG - chr4_3:470954-470973 Msa0623290:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG - chr4_3:471617-471636 Msa0623290:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG + chr4_3:471972-471991 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG + chr4_3:471973-471992 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG - chr4_3:472007-472026 Msa0623290:CDS 45.0%
TTATCAGCCACGTGGAACAC+TGG - chr4_3:469873-469892 Msa0623290:three_prime_UTR 50.0%
TATCAGCCACGTGGAACACT+GGG - chr4_3:469874-469893 Msa0623290:three_prime_UTR 50.0%
! TAAAGTGTGACGGGGTGGTT+GGG + chr4_3:469904-469923 None:intergenic 50.0%
! ATAAAGTGTGACGGGGTGGT+TGG + chr4_3:469905-469924 None:intergenic 50.0%
CGCTTCCATGCATTCTTCCT+TGG + chr4_3:470291-470310 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG + chr4_3:470654-470673 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG - chr4_3:471708-471727 Msa0623290:intron 50.0%
CACACACGCATGCTAGTGTT+TGG + chr4_3:471787-471806 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG + chr4_3:472023-472042 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG + chr4_3:472165-472184 None:intergenic 50.0%
CATGGGGGACTCGTCTTCTT+CGG - chr4_3:470079-470098 Msa0623290:CDS 55.0%
AGGAATAGGCTGCGCGATCT+AGG - chr4_3:470974-470993 Msa0623290:intron 55.0%
! GAGTCCCCCATGCTAGCTCT+TGG + chr4_3:470071-470090 None:intergenic 60.0%
! GTGTGACGGGGTGGTTGGGT+GGG + chr4_3:469900-469919 None:intergenic 65.0%
! AGTGTGACGGGGTGGTTGGG+TGG + chr4_3:469901-469920 None:intergenic 65.0%
GGTGGGCCCAGTGTTCCACG+TGG + chr4_3:469883-469902 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 469790 472319 469790 ID=Msa0623290;Name=Msa0623290
chr4_3 mRNA 469790 472319 469790 ID=Msa0623290-mRNA-1;Parent=Msa0623290;Name=Msa0623290-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.66|0.5|0.75|0|0.25|4|271|113
chr4_3 exon 469790 470224 469790 ID=Msa0623290-mRNA-1:exon:144;Parent=Msa0623290-mRNA-1
chr4_3 exon 471756 471861 471756 ID=Msa0623290-mRNA-1:exon:143;Parent=Msa0623290-mRNA-1
chr4_3 exon 471991 472054 471991 ID=Msa0623290-mRNA-1:exon:142;Parent=Msa0623290-mRNA-1
chr4_3 exon 472312 472319 472312 ID=Msa0623290-mRNA-1:exon:141;Parent=Msa0623290-mRNA-1
chr4_3 CDS 472312 472319 472312 ID=Msa0623290-mRNA-1:cds;Parent=Msa0623290-mRNA-1
chr4_3 CDS 471991 472054 471991 ID=Msa0623290-mRNA-1:cds;Parent=Msa0623290-mRNA-1
chr4_3 CDS 471756 471861 471756 ID=Msa0623290-mRNA-1:cds;Parent=Msa0623290-mRNA-1
chr4_3 CDS 470061 470224 470061 ID=Msa0623290-mRNA-1:cds;Parent=Msa0623290-mRNA-1
chr4_3 three_prime_UTR 469790 470060 469790 ID=Msa0623290-mRNA-1:three_prime_utr;Parent=Msa0623290-mRNA-1
Gene Sequence

>Msa0623290

ATGGAATTACTACGTGCAATACCAAGAGCTAGCATGGGGGACTCGTCTTCTTCGGCTTCATACATTCACTTGGTGCAGCACCTGATCGAAAAGTGTTTGATCTTTCACATGACCAAGGAAGAATGCATGGAAGCGCTCTCCAAACATGCAAATATAAATCCAATTATAACATCCACTGTGTGGAATGAGTTAGAGAAGGTGAACAAGGAATTTTTTGAGCCATATAACATGAAGCCAAAGAGTAGTGAAGAGAAAATGATGTCCCAGGAAGAGACAACTCAGATGTTAAAGAAGATGATCTCAGCTTCGGATTCCTCTAAAGGTGCACAACACGACGACTAA

Protein sequence

>Msa0623290

MELLRAIPRASMGDSSSSASYIHLVQHLIEKCLIFHMTKEECMEALSKHANINPIITSTVWNELEKVNKEFFEPYNMKPKSSEEKMMSQEETTQMLKKMISASDSSKGAQHDD*