AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1376330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376330 MtrunA17_Chr1g0212831 93.000 100 5 1 1 98 1 100 1.92e-61 182
Msa1376330 MtrunA17_Chr7g0222601 76.087 92 18 3 10 101 15 102 2.87e-42 134
Msa1376330 MtrunA17_Chr7g0263161 59.140 93 28 3 6 96 4 88 5.84e-31 105
Msa1376330 MtrunA17_Chr8g0368511 57.143 56 24 0 9 64 48 103 4.50e-20 78.2
Msa1376330 MtrunA17_Chr1g0171941 45.614 57 31 0 11 67 14 70 7.77e-12 59.7
Msa1376330 MtrunA17_Chr8g0347131 43.860 57 32 0 11 67 55 111 2.66e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1376330 AT3G55240.1 69.792 96 24 3 1 96 1 91 5.93e-41 130
Msa1376330 AT3G28990.1 65.116 86 28 1 5 90 4 87 1.92e-32 108
Msa1376330 AT5G02580.1 56.989 93 35 2 1 92 1 89 2.98e-32 108
Msa1376330 AT5G02580.3 53.165 79 33 1 14 92 8 82 1.61e-24 89.0
Msa1376330 AT5G02580.2 75.510 49 11 1 1 48 1 49 6.24e-20 76.6
Msa1376330 AT1G10657.2 52.632 57 27 0 9 65 22 78 1.51e-19 76.6
Msa1376330 AT1G10657.3 52.632 57 27 0 9 65 22 78 1.85e-19 76.6
Msa1376330 AT1G10657.1 37.037 81 27 1 9 65 22 102 5.60e-14 63.2
Msa1376330 AT1G10657.4 37.037 81 27 1 9 65 22 102 7.48e-14 62.8
Msa1376330 AT2G46420.3 43.860 57 32 0 11 67 52 108 6.24e-12 60.5
Msa1376330 AT2G46420.1 43.860 57 32 0 11 67 52 108 6.24e-12 60.5
Msa1376330 AT2G46420.2 43.860 57 32 0 11 67 52 108 6.68e-12 60.5
Msa1376330 AT5G04090.6 43.860 57 32 0 11 67 31 87 7.04e-12 60.5
Msa1376330 AT5G04090.2 43.860 57 32 0 11 67 14 70 7.95e-12 60.1
Msa1376330 AT3G10250.2 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1376330 AT3G10250.5 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1376330 AT3G10250.1 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1376330 AT3G10250.4 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1376330 AT3G10250.3 43.860 57 32 0 11 67 14 70 1.64e-11 59.3

Find 32 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATATATATGACCAATTT+AGG 0.171764 tig0019789:+146928 Msa1376330:three_prime_UTR
TTTCAAGAGTTGATTGATTA+TGG 0.214655 tig0019789:-146905 None:intergenic
ATACCAGTGGATGTTATAAT+TGG 0.219027 tig0019789:-145192 None:intergenic
CTCTTTGGCTTCATGTTATA+TGG 0.220781 tig0019789:-146780 None:intergenic
ACATCTGAGTTGTCTCTTCC+TGG 0.233068 tig0019789:-146824 None:intergenic
TGGATTTATATTTGCATGTT+TGG 0.252690 tig0019789:-145172 None:intergenic
GTTGCTCCTCTACATTATTA+TGG 0.253456 tig0019789:+147060 Msa1376330:three_prime_UTR
CGCTTCCATGCATTCTTCCT+TGG 0.321815 tig0019789:-145145 None:intergenic
TGCATGGTAAGTACCTAAAT+TGG 0.364202 tig0019789:-146941 None:intergenic
TGACCCTTGTATATATTTGA+TGG 0.370068 tig0019789:-144841 None:intergenic
GTCGTGTTGTGCACCTTTAG+AGG 0.436240 tig0019789:-146874 None:intergenic
AAAGAAGATGATCTCAGCTT+CGG 0.465702 tig0019789:+146848 Msa1376330:CDS
TCAGCTTCGGATTCCTCTAA+AGG 0.506755 tig0019789:+146861 Msa1376330:CDS
TTCGGCTTCATACATTCACT+TGG 0.510731 tig0019789:+144954 Msa1376330:CDS
CATCTGAGTTGTCTCTTCCT+GGG 0.513019 tig0019789:-146823 None:intergenic
TGCAATACCAAGAGCTAGCA+TGG 0.513676 tig0019789:+144918 Msa1376330:exon
TGAGTTAGAGAAGGTGAACA+AGG 0.535925 tig0019789:+146746 Msa1376330:CDS
AATCCAATTATAACATCCAC+TGG 0.539693 tig0019789:+145189 Msa1376330:CDS
AAACATATTTATCAAGAACT+AGG 0.557315 tig0019789:+144866 Msa1376330:five_prime_UTR
AGTTCCATCAAATATATACA+AGG 0.559883 tig0019789:+144837 Msa1376330:five_prime_UTR
CATGACCAAGGAAGAATGCA+TGG 0.570865 tig0019789:+145140 Msa1376330:CDS
TGAAGAGAAAATGATGTCCC+AGG 0.581534 tig0019789:+146806 Msa1376330:CDS
GCAATACCAAGAGCTAGCAT+GGG 0.596506 tig0019789:+144919 Msa1376330:exon
GCGCATAGACTACTAGCACT+AGG 0.603360 tig0019789:-147016 None:intergenic
ACAATACCATAATAATGTAG+AGG 0.622036 tig0019789:-147066 None:intergenic
AGTGTGGAATGAGTTAGAGA+AGG 0.642407 tig0019789:+146737 Msa1376330:intron
GAATATTATATATACTACAA+AGG 0.655145 tig0019789:-147122 None:intergenic
TTTGATCTTTCACATGACCA+AGG 0.655387 tig0019789:+145128 Msa1376330:CDS
GTTCCATCAAATATATACAA+GGG 0.664258 tig0019789:+144838 Msa1376330:five_prime_UTR
CAATACCAAGAGCTAGCATG+GGG 0.665262 tig0019789:+144920 Msa1376330:exon
ATATACTACAAAGGTTGACA+AGG 0.676891 tig0019789:-147113 None:intergenic
AATACCAAGAGCTAGCATGG+GGG 0.797315 tig0019789:+144921 Msa1376330:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATCAAATCTT+AGG + tig0019789:145881-145900 Msa1376330:intron 10.0%
!! TATAATAATATGTTTACATA+CGG + tig0019789:146162-146181 Msa1376330:intron 10.0%
!!! AAAACACAATTTTTTTTTTT+CGG - tig0019789:146439-146458 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG + tig0019789:146513-146532 Msa1376330:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG + tig0019789:146514-146533 Msa1376330:intron 10.0%
!! AAAAAAAAAAATCAAATCTT+AGG + tig0019789:145881-145900 Msa1376330:intron 10.0%
!! TATAATAATATGTTTACATA+CGG + tig0019789:146162-146181 Msa1376330:intron 10.0%
!!! AAAACACAATTTTTTTTTTT+CGG - tig0019789:146439-146458 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG + tig0019789:146513-146532 Msa1376330:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG + tig0019789:146514-146533 Msa1376330:intron 10.0%
!!! ATCTACTATTTTACTAAAAA+TGG + tig0019789:145423-145442 Msa1376330:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG + tig0019789:145919-145938 Msa1376330:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG + tig0019789:146163-146182 Msa1376330:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG + tig0019789:146541-146560 Msa1376330:intron 15.0%
!! ATTATATATATGACCAATTT+AGG + tig0019789:146928-146947 Msa1376330:three_prime_UTR 15.0%
!!! GTTTTGATAATAATATAGTA+GGG - tig0019789:146997-147016 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG - tig0019789:147125-147144 None:intergenic 15.0%
!!! ATCTACTATTTTACTAAAAA+TGG + tig0019789:145423-145442 Msa1376330:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG + tig0019789:145919-145938 Msa1376330:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG + tig0019789:146163-146182 Msa1376330:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG + tig0019789:146541-146560 Msa1376330:intron 15.0%
!! ATTATATATATGACCAATTT+AGG + tig0019789:146928-146947 Msa1376330:three_prime_UTR 15.0%
!!! GTTTTGATAATAATATAGTA+GGG - tig0019789:146997-147016 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG - tig0019789:147125-147144 None:intergenic 15.0%
!! AAACATATTTATCAAGAACT+AGG + tig0019789:144866-144885 Msa1376330:five_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG + tig0019789:145083-145102 Msa1376330:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG + tig0019789:145241-145260 Msa1376330:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG - tig0019789:145377-145396 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG - tig0019789:145559-145578 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG + tig0019789:145587-145606 Msa1376330:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG - tig0019789:145689-145708 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG - tig0019789:146998-147017 None:intergenic 20.0%
!! AAACATATTTATCAAGAACT+AGG + tig0019789:144866-144885 Msa1376330:five_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG + tig0019789:145083-145102 Msa1376330:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG + tig0019789:145241-145260 Msa1376330:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG - tig0019789:145377-145396 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG - tig0019789:145559-145578 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG + tig0019789:145587-145606 Msa1376330:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG - tig0019789:145689-145708 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG - tig0019789:146998-147017 None:intergenic 20.0%
! AGTTCCATCAAATATATACA+AGG + tig0019789:144837-144856 Msa1376330:five_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG + tig0019789:144838-144857 Msa1376330:five_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG - tig0019789:145057-145076 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG - tig0019789:145175-145194 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG - tig0019789:145208-145227 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG + tig0019789:145454-145473 Msa1376330:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG + tig0019789:145586-145605 Msa1376330:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG + tig0019789:145588-145607 Msa1376330:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG + tig0019789:145589-145608 Msa1376330:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG - tig0019789:145645-145664 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG + tig0019789:145947-145966 Msa1376330:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG - tig0019789:146081-146100 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG - tig0019789:146096-146115 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG + tig0019789:146298-146317 Msa1376330:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG - tig0019789:146392-146411 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG + tig0019789:146669-146688 Msa1376330:intron 25.0%
! ATGAAATAATGAATGTAGTG+TGG + tig0019789:146721-146740 Msa1376330:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG - tig0019789:146908-146927 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG - tig0019789:147069-147088 None:intergenic 25.0%
! AGTTCCATCAAATATATACA+AGG + tig0019789:144837-144856 Msa1376330:five_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG + tig0019789:144838-144857 Msa1376330:five_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG - tig0019789:145057-145076 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG - tig0019789:145175-145194 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG - tig0019789:145208-145227 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG + tig0019789:145454-145473 Msa1376330:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG + tig0019789:145586-145605 Msa1376330:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG + tig0019789:145588-145607 Msa1376330:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG + tig0019789:145589-145608 Msa1376330:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG - tig0019789:145645-145664 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG + tig0019789:145947-145966 Msa1376330:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG - tig0019789:146081-146100 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG - tig0019789:146096-146115 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG + tig0019789:146298-146317 Msa1376330:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG - tig0019789:146392-146411 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG + tig0019789:146669-146688 Msa1376330:intron 25.0%
! ATGAAATAATGAATGTAGTG+TGG + tig0019789:146721-146740 Msa1376330:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG - tig0019789:146908-146927 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG - tig0019789:147069-147088 None:intergenic 25.0%
TGACCCTTGTATATATTTGA+TGG - tig0019789:144844-144863 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG + tig0019789:145189-145208 Msa1376330:CDS 30.0%
ATACCAGTGGATGTTATAAT+TGG - tig0019789:145195-145214 None:intergenic 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG + tig0019789:145848-145867 Msa1376330:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG + tig0019789:145961-145980 Msa1376330:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG - tig0019789:146393-146412 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG + tig0019789:146403-146422 Msa1376330:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG + tig0019789:146607-146626 Msa1376330:intron 30.0%
CTCATGAACTGAAATTACAT+TGG + tig0019789:146668-146687 Msa1376330:intron 30.0%
! ATTTTCTCTTCACTACTCTT+TGG - tig0019789:146798-146817 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG - tig0019789:146972-146991 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG - tig0019789:147116-147135 None:intergenic 30.0%
TGACCCTTGTATATATTTGA+TGG - tig0019789:144844-144863 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG + tig0019789:145189-145208 Msa1376330:CDS 30.0%
ATACCAGTGGATGTTATAAT+TGG - tig0019789:145195-145214 None:intergenic 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG + tig0019789:145848-145867 Msa1376330:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG + tig0019789:145961-145980 Msa1376330:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG - tig0019789:146393-146412 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG + tig0019789:146403-146422 Msa1376330:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG + tig0019789:146607-146626 Msa1376330:intron 30.0%
CTCATGAACTGAAATTACAT+TGG + tig0019789:146668-146687 Msa1376330:intron 30.0%
! ATTTTCTCTTCACTACTCTT+TGG - tig0019789:146798-146817 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG - tig0019789:146972-146991 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG - tig0019789:147116-147135 None:intergenic 30.0%
! AGATCAAACACTTTTCGATC+AGG - tig0019789:145116-145135 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG + tig0019789:145128-145147 Msa1376330:CDS 35.0%
CCAATTGGTTAACAATCAAG+TGG - tig0019789:145496-145515 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG + tig0019789:145493-145512 Msa1376330:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG + tig0019789:145501-145520 Msa1376330:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG + tig0019789:145502-145521 Msa1376330:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG - tig0019789:145683-145702 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG + tig0019789:145750-145769 Msa1376330:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG - tig0019789:145783-145802 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG - tig0019789:145784-145803 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG + tig0019789:145781-145800 Msa1376330:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG - tig0019789:145785-145804 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG + tig0019789:146407-146426 Msa1376330:intron 35.0%
TAGATCTACTCATCTAGACA+AGG - tig0019789:146463-146482 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG + tig0019789:146465-146484 Msa1376330:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG + tig0019789:146558-146577 Msa1376330:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG - tig0019789:146783-146802 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG + tig0019789:146848-146867 Msa1376330:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG - tig0019789:146944-146963 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG - tig0019789:146968-146987 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG + tig0019789:146966-146985 Msa1376330:three_prime_UTR 35.0%
GTTGCTCCTCTACATTATTA+TGG + tig0019789:147060-147079 Msa1376330:three_prime_UTR 35.0%
! AGATCAAACACTTTTCGATC+AGG - tig0019789:145116-145135 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG + tig0019789:145128-145147 Msa1376330:CDS 35.0%
CCAATTGGTTAACAATCAAG+TGG - tig0019789:145496-145515 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG + tig0019789:145493-145512 Msa1376330:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG + tig0019789:145501-145520 Msa1376330:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG + tig0019789:145502-145521 Msa1376330:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG - tig0019789:145683-145702 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG + tig0019789:145750-145769 Msa1376330:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG - tig0019789:145783-145802 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG - tig0019789:145784-145803 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG + tig0019789:145781-145800 Msa1376330:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG - tig0019789:145785-145804 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG + tig0019789:146407-146426 Msa1376330:intron 35.0%
TAGATCTACTCATCTAGACA+AGG - tig0019789:146463-146482 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG + tig0019789:146465-146484 Msa1376330:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG + tig0019789:146558-146577 Msa1376330:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG - tig0019789:146783-146802 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG + tig0019789:146848-146867 Msa1376330:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG - tig0019789:146944-146963 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG - tig0019789:146968-146987 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG + tig0019789:146966-146985 Msa1376330:three_prime_UTR 35.0%
GTTGCTCCTCTACATTATTA+TGG + tig0019789:147060-147079 Msa1376330:three_prime_UTR 35.0%
TTCGGCTTCATACATTCACT+TGG + tig0019789:144954-144973 Msa1376330:CDS 40.0%
! CATGTCTAGAGCTAACTTAG+AGG + tig0019789:145706-145725 Msa1376330:intron 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG - tig0019789:145772-145791 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG + tig0019789:145780-145799 Msa1376330:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG + tig0019789:145802-145821 Msa1376330:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG + tig0019789:145803-145822 Msa1376330:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG + tig0019789:145813-145832 Msa1376330:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG + tig0019789:145837-145856 Msa1376330:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG - tig0019789:146414-146433 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG + tig0019789:146466-146485 Msa1376330:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG + tig0019789:146580-146599 Msa1376330:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG + tig0019789:146737-146756 Msa1376330:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG + tig0019789:146746-146765 Msa1376330:CDS 40.0%
TGAAGAGAAAATGATGTCCC+AGG + tig0019789:146806-146825 Msa1376330:CDS 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG - tig0019789:146960-146979 None:intergenic 40.0%
TTCGGCTTCATACATTCACT+TGG + tig0019789:144954-144973 Msa1376330:CDS 40.0%
! CATGTCTAGAGCTAACTTAG+AGG + tig0019789:145706-145725 Msa1376330:intron 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG - tig0019789:145772-145791 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG + tig0019789:145780-145799 Msa1376330:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG + tig0019789:145802-145821 Msa1376330:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG + tig0019789:145803-145822 Msa1376330:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG + tig0019789:145813-145832 Msa1376330:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG + tig0019789:145837-145856 Msa1376330:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG - tig0019789:146414-146433 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG + tig0019789:146466-146485 Msa1376330:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG + tig0019789:146580-146599 Msa1376330:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG + tig0019789:146737-146756 Msa1376330:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG + tig0019789:146746-146765 Msa1376330:CDS 40.0%
TGAAGAGAAAATGATGTCCC+AGG + tig0019789:146806-146825 Msa1376330:CDS 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG - tig0019789:146960-146979 None:intergenic 40.0%
TGCAATACCAAGAGCTAGCA+TGG + tig0019789:144918-144937 Msa1376330:exon 45.0%
GCAATACCAAGAGCTAGCAT+GGG + tig0019789:144919-144938 Msa1376330:exon 45.0%
CAATACCAAGAGCTAGCATG+GGG + tig0019789:144920-144939 Msa1376330:exon 45.0%
AATACCAAGAGCTAGCATGG+GGG + tig0019789:144921-144940 Msa1376330:exon 45.0%
CATGACCAAGGAAGAATGCA+TGG + tig0019789:145140-145159 Msa1376330:CDS 45.0%
GCGTGCATGCTTAAGAAGAT+AGG + tig0019789:145529-145548 Msa1376330:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG + tig0019789:145807-145826 Msa1376330:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG + tig0019789:146471-146490 Msa1376330:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG - tig0019789:146826-146845 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG - tig0019789:146827-146846 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG + tig0019789:146861-146880 Msa1376330:CDS 45.0%
TGCAATACCAAGAGCTAGCA+TGG + tig0019789:144918-144937 Msa1376330:exon 45.0%
GCAATACCAAGAGCTAGCAT+GGG + tig0019789:144919-144938 Msa1376330:exon 45.0%
CAATACCAAGAGCTAGCATG+GGG + tig0019789:144920-144939 Msa1376330:exon 45.0%
AATACCAAGAGCTAGCATGG+GGG + tig0019789:144921-144940 Msa1376330:exon 45.0%
CATGACCAAGGAAGAATGCA+TGG + tig0019789:145140-145159 Msa1376330:CDS 45.0%
GCGTGCATGCTTAAGAAGAT+AGG + tig0019789:145529-145548 Msa1376330:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG + tig0019789:145807-145826 Msa1376330:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG + tig0019789:146471-146490 Msa1376330:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG - tig0019789:146826-146845 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG - tig0019789:146827-146846 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG + tig0019789:146861-146880 Msa1376330:CDS 45.0%
CGCTTCCATGCATTCTTCCT+TGG - tig0019789:145148-145167 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG - tig0019789:145511-145530 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG + tig0019789:146562-146581 Msa1376330:intron 50.0%
CACACACGCATGCTAGTGTT+TGG - tig0019789:146641-146660 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG - tig0019789:146877-146896 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG - tig0019789:147019-147038 None:intergenic 50.0%
CGCTTCCATGCATTCTTCCT+TGG - tig0019789:145148-145167 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG - tig0019789:145511-145530 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG + tig0019789:146562-146581 Msa1376330:intron 50.0%
CACACACGCATGCTAGTGTT+TGG - tig0019789:146641-146660 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG - tig0019789:146877-146896 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG - tig0019789:147019-147038 None:intergenic 50.0%
CATGGGGGACTCGTCTTCTT+CGG + tig0019789:144936-144955 Msa1376330:exon 55.0%
AGGAATAGGCTGCGCGATCT+AGG + tig0019789:145827-145846 Msa1376330:intron 55.0%
CATGGGGGACTCGTCTTCTT+CGG + tig0019789:144936-144955 Msa1376330:exon 55.0%
AGGAATAGGCTGCGCGATCT+AGG + tig0019789:145827-145846 Msa1376330:intron 55.0%
! GAGTCCCCCATGCTAGCTCT+TGG - tig0019789:144928-144947 None:intergenic 60.0%
! GAGTCCCCCATGCTAGCTCT+TGG - tig0019789:144928-144947 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0019789 gene 144834 147173 144834 ID=Msa1376330;Name=Msa1376330
tig0019789 mRNA 144834 147173 144834 ID=Msa1376330-mRNA-1;Parent=Msa1376330;Name=Msa1376330-mRNA-1;_AED=0.09;_eAED=0.09;_QI=103|1|1|1|0|0|3|271|102
tig0019789 exon 144834 144975 144834 ID=Msa1376330-mRNA-1:exon:4986;Parent=Msa1376330-mRNA-1
tig0019789 exon 145105 145210 145105 ID=Msa1376330-mRNA-1:exon:4987;Parent=Msa1376330-mRNA-1
tig0019789 exon 146739 147173 146739 ID=Msa1376330-mRNA-1:exon:4988;Parent=Msa1376330-mRNA-1
tig0019789 five_prime_UTR 144834 144936 144834 ID=Msa1376330-mRNA-1:five_prime_utr;Parent=Msa1376330-mRNA-1
tig0019789 CDS 144937 144975 144937 ID=Msa1376330-mRNA-1:cds;Parent=Msa1376330-mRNA-1
tig0019789 CDS 145105 145210 145105 ID=Msa1376330-mRNA-1:cds;Parent=Msa1376330-mRNA-1
tig0019789 CDS 146739 146902 146739 ID=Msa1376330-mRNA-1:cds;Parent=Msa1376330-mRNA-1
tig0019789 three_prime_UTR 146903 147173 146903 ID=Msa1376330-mRNA-1:three_prime_utr;Parent=Msa1376330-mRNA-1
Gene Sequence

>Msa1376330

ATGGGGGACTCGTCTTCTTCGGCTTCATACATTCACTTGGTGCAGCACCTGATCGAAAAGTGTTTGATCTTTCACATGACCAAGGAAGAATGCATGGAAGCGCTCTCCAAACATGCAAATATAAATCCAATTATAACATCCACTGTGTGGAATGAGTTAGAGAAGGTGAACAAGGAATTTTTTGAGCCATATAACATGAAGCCAAAGAGTAGTGAAGAGAAAATGATGTCCCAGGAAGAGACAACTCAGATGTTAAAGAAGATGATCTCAGCTTCGGATTCCTCTAAAGGTGCACAACACGACGACTAA
Protein sequence

>Msa1376330

MGDSSSSASYIHLVQHLIEKCLIFHMTKEECMEALSKHANINPIITSTVWNELEKVNKEFFEPYNMKPKSSEEKMMSQEETTQMLKKMISASDSSKGAQHDD*