AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1371820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371820 MtrunA17_Chr1g0212831 93.000 100 5 1 1 98 1 100 1.92e-61 182
Msa1371820 MtrunA17_Chr7g0222601 76.087 92 18 3 10 101 15 102 2.87e-42 134
Msa1371820 MtrunA17_Chr7g0263161 59.140 93 28 3 6 96 4 88 5.84e-31 105
Msa1371820 MtrunA17_Chr8g0368511 57.143 56 24 0 9 64 48 103 4.50e-20 78.2
Msa1371820 MtrunA17_Chr1g0171941 45.614 57 31 0 11 67 14 70 7.77e-12 59.7
Msa1371820 MtrunA17_Chr8g0347131 43.860 57 32 0 11 67 55 111 2.66e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371820 AT3G55240.1 69.792 96 24 3 1 96 1 91 5.93e-41 130
Msa1371820 AT3G28990.1 65.116 86 28 1 5 90 4 87 1.92e-32 108
Msa1371820 AT5G02580.1 56.989 93 35 2 1 92 1 89 2.98e-32 108
Msa1371820 AT5G02580.3 53.165 79 33 1 14 92 8 82 1.61e-24 89.0
Msa1371820 AT5G02580.2 75.510 49 11 1 1 48 1 49 6.24e-20 76.6
Msa1371820 AT1G10657.2 52.632 57 27 0 9 65 22 78 1.51e-19 76.6
Msa1371820 AT1G10657.3 52.632 57 27 0 9 65 22 78 1.85e-19 76.6
Msa1371820 AT1G10657.1 37.037 81 27 1 9 65 22 102 5.60e-14 63.2
Msa1371820 AT1G10657.4 37.037 81 27 1 9 65 22 102 7.48e-14 62.8
Msa1371820 AT2G46420.3 43.860 57 32 0 11 67 52 108 6.24e-12 60.5
Msa1371820 AT2G46420.1 43.860 57 32 0 11 67 52 108 6.24e-12 60.5
Msa1371820 AT2G46420.2 43.860 57 32 0 11 67 52 108 6.68e-12 60.5
Msa1371820 AT5G04090.6 43.860 57 32 0 11 67 31 87 7.04e-12 60.5
Msa1371820 AT5G04090.2 43.860 57 32 0 11 67 14 70 7.95e-12 60.1
Msa1371820 AT3G10250.2 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1371820 AT3G10250.5 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1371820 AT3G10250.1 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1371820 AT3G10250.4 43.860 57 32 0 11 67 14 70 1.07e-11 59.7
Msa1371820 AT3G10250.3 43.860 57 32 0 11 67 14 70 1.64e-11 59.3

Find 32 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATATATATGACCAATTT+AGG 0.171764 tig0019249:+161602 Msa1371820:three_prime_UTR
TTTCAAGAGTTGATTGATTA+TGG 0.214655 tig0019249:-161579 None:intergenic
ATACCAGTGGATGTTATAAT+TGG 0.219027 tig0019249:-159867 None:intergenic
CTCTTTGGCTTCATGTTATA+TGG 0.220781 tig0019249:-161454 None:intergenic
ACATCTGAGTTGTCTCTTCC+TGG 0.233068 tig0019249:-161498 None:intergenic
TGGATTTATATTTGCATGTT+TGG 0.252690 tig0019249:-159847 None:intergenic
GTTGCTCCTCTACATTATTA+TGG 0.253456 tig0019249:+161734 Msa1371820:three_prime_UTR
CGCTTCCATGCATTCTTCCT+TGG 0.321815 tig0019249:-159820 None:intergenic
TGCATGGTAAGTACCTAAAT+TGG 0.364202 tig0019249:-161615 None:intergenic
TGACCCTTGTATATATTTGA+TGG 0.370068 tig0019249:-159516 None:intergenic
GTCGTGTTGTGCACCTTTAG+AGG 0.436240 tig0019249:-161548 None:intergenic
AAAGAAGATGATCTCAGCTT+CGG 0.465702 tig0019249:+161522 Msa1371820:CDS
TCAGCTTCGGATTCCTCTAA+AGG 0.506755 tig0019249:+161535 Msa1371820:CDS
TTCGGCTTCATACATTCACT+TGG 0.510731 tig0019249:+159629 Msa1371820:CDS
CATCTGAGTTGTCTCTTCCT+GGG 0.513019 tig0019249:-161497 None:intergenic
TGCAATACCAAGAGCTAGCA+TGG 0.513676 tig0019249:+159593 Msa1371820:exon
TGAGTTAGAGAAGGTGAACA+AGG 0.535925 tig0019249:+161420 Msa1371820:CDS
AATCCAATTATAACATCCAC+TGG 0.539693 tig0019249:+159864 Msa1371820:CDS
AAACATATTTATCAAGAACT+AGG 0.557315 tig0019249:+159541 Msa1371820:five_prime_UTR
AGTTCCATCAAATATATACA+AGG 0.559883 tig0019249:+159512 Msa1371820:five_prime_UTR
CATGACCAAGGAAGAATGCA+TGG 0.570865 tig0019249:+159815 Msa1371820:CDS
TGAAGAGAAAATGATGTCCC+AGG 0.581534 tig0019249:+161480 Msa1371820:CDS
GCAATACCAAGAGCTAGCAT+GGG 0.596506 tig0019249:+159594 Msa1371820:exon
GCGCATAGACTACTAGCACT+AGG 0.603360 tig0019249:-161690 None:intergenic
ACAATACCATAATAATGTAG+AGG 0.622036 tig0019249:-161740 None:intergenic
AGTGTGGAATGAGTTAGAGA+AGG 0.642407 tig0019249:+161411 Msa1371820:intron
GAATATTATATATACTACAA+AGG 0.655145 tig0019249:-161796 None:intergenic
TTTGATCTTTCACATGACCA+AGG 0.655387 tig0019249:+159803 Msa1371820:CDS
GTTCCATCAAATATATACAA+GGG 0.664258 tig0019249:+159513 Msa1371820:five_prime_UTR
CAATACCAAGAGCTAGCATG+GGG 0.665262 tig0019249:+159595 Msa1371820:exon
ATATACTACAAAGGTTGACA+AGG 0.676891 tig0019249:-161787 None:intergenic
AATACCAAGAGCTAGCATGG+GGG 0.797315 tig0019249:+159596 Msa1371820:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATCAAATCTT+AGG + tig0019249:160555-160574 Msa1371820:intron 10.0%
!! TATAATAATATGTTTACATA+CGG + tig0019249:160836-160855 Msa1371820:intron 10.0%
!!! AAAACACAATTTTTTTTTTT+CGG - tig0019249:161113-161132 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG + tig0019249:161187-161206 Msa1371820:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG + tig0019249:161188-161207 Msa1371820:intron 10.0%
!! AAAAAAAAAAATCAAATCTT+AGG + tig0019249:160555-160574 Msa1371820:intron 10.0%
!! TATAATAATATGTTTACATA+CGG + tig0019249:160836-160855 Msa1371820:intron 10.0%
!!! AAAACACAATTTTTTTTTTT+CGG - tig0019249:161113-161132 None:intergenic 10.0%
!! AAAACATTTCTATTTAAATA+AGG + tig0019249:161187-161206 Msa1371820:intron 10.0%
!! AAACATTTCTATTTAAATAA+GGG + tig0019249:161188-161207 Msa1371820:intron 10.0%
!!! ATCTACTATTTTACTAAAAA+TGG + tig0019249:160098-160117 Msa1371820:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG + tig0019249:160593-160612 Msa1371820:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG + tig0019249:160837-160856 Msa1371820:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG + tig0019249:161215-161234 Msa1371820:intron 15.0%
!! ATTATATATATGACCAATTT+AGG + tig0019249:161602-161621 Msa1371820:three_prime_UTR 15.0%
!!! GTTTTGATAATAATATAGTA+GGG - tig0019249:161671-161690 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG - tig0019249:161799-161818 None:intergenic 15.0%
!!! ATCTACTATTTTACTAAAAA+TGG + tig0019249:160098-160117 Msa1371820:intron 15.0%
!!! TCTATACTAGATTTATTTTT+AGG + tig0019249:160593-160612 Msa1371820:intron 15.0%
!! ATAATAATATGTTTACATAC+GGG + tig0019249:160837-160856 Msa1371820:intron 15.0%
!! ATTTGCATTTAATGTAAATT+AGG + tig0019249:161215-161234 Msa1371820:intron 15.0%
!! ATTATATATATGACCAATTT+AGG + tig0019249:161602-161621 Msa1371820:three_prime_UTR 15.0%
!!! GTTTTGATAATAATATAGTA+GGG - tig0019249:161671-161690 None:intergenic 15.0%
!! GAATATTATATATACTACAA+AGG - tig0019249:161799-161818 None:intergenic 15.0%
!! AAACATATTTATCAAGAACT+AGG + tig0019249:159541-159560 Msa1371820:five_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG + tig0019249:159758-159777 Msa1371820:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG + tig0019249:159916-159935 Msa1371820:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG - tig0019249:160052-160071 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG - tig0019249:160234-160253 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG + tig0019249:160262-160281 Msa1371820:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG - tig0019249:160364-160383 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG - tig0019249:161672-161691 None:intergenic 20.0%
!! AAACATATTTATCAAGAACT+AGG + tig0019249:159541-159560 Msa1371820:five_prime_UTR 20.0%
!! CTACAATTGCAAATATAATT+AGG + tig0019249:159758-159777 Msa1371820:intron 20.0%
!! TAACAACAAATACGTAAATA+TGG + tig0019249:159916-159935 Msa1371820:intron 20.0%
!! AATCAAAAGCTAGATATATA+TGG - tig0019249:160052-160071 None:intergenic 20.0%
!! TGTCTAAAAAAGAAAATCAT+TGG - tig0019249:160234-160253 None:intergenic 20.0%
!!! TGATTACTTATAGTTTGTTA+GGG + tig0019249:160262-160281 Msa1371820:intron 20.0%
!! ATGAATGAACTATATATCAT+TGG - tig0019249:160364-160383 None:intergenic 20.0%
!!! GGTTTTGATAATAATATAGT+AGG - tig0019249:161672-161691 None:intergenic 20.0%
! AGTTCCATCAAATATATACA+AGG + tig0019249:159512-159531 Msa1371820:five_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG + tig0019249:159513-159532 Msa1371820:five_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG - tig0019249:159732-159751 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG - tig0019249:159850-159869 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG - tig0019249:159883-159902 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG + tig0019249:160129-160148 Msa1371820:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG + tig0019249:160261-160280 Msa1371820:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG + tig0019249:160263-160282 Msa1371820:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG + tig0019249:160264-160283 Msa1371820:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG - tig0019249:160320-160339 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG + tig0019249:160621-160640 Msa1371820:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG - tig0019249:160755-160774 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG - tig0019249:160770-160789 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG + tig0019249:160972-160991 Msa1371820:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG - tig0019249:161066-161085 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG + tig0019249:161343-161362 Msa1371820:intron 25.0%
! ATGAAATAATGAATGTAGTG+TGG + tig0019249:161395-161414 Msa1371820:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG - tig0019249:161582-161601 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG - tig0019249:161743-161762 None:intergenic 25.0%
! AGTTCCATCAAATATATACA+AGG + tig0019249:159512-159531 Msa1371820:five_prime_UTR 25.0%
! GTTCCATCAAATATATACAA+GGG + tig0019249:159513-159532 Msa1371820:five_prime_UTR 25.0%
!! ATAATTTTTGTCAACATGCA+TGG - tig0019249:159732-159751 None:intergenic 25.0%
! TGGATTTATATTTGCATGTT+TGG - tig0019249:159850-159869 None:intergenic 25.0%
! TTAAAAATCATACATACCAG+TGG - tig0019249:159883-159902 None:intergenic 25.0%
!!! AATCTTTAGACTATTTCTGA+AGG + tig0019249:160129-160148 Msa1371820:intron 25.0%
!!! GTGATTACTTATAGTTTGTT+AGG + tig0019249:160261-160280 Msa1371820:intron 25.0%
!!! GATTACTTATAGTTTGTTAG+GGG + tig0019249:160263-160282 Msa1371820:intron 25.0%
!!! ATTACTTATAGTTTGTTAGG+GGG + tig0019249:160264-160283 Msa1371820:intron 25.0%
!! ATATGTAAGAAGTGTATAGT+TGG - tig0019249:160320-160339 None:intergenic 25.0%
!!! GTCTAACATTTTTTATAGTC+TGG + tig0019249:160621-160640 Msa1371820:intron 25.0%
! ATCATTGGTAAAAACATATG+AGG - tig0019249:160755-160774 None:intergenic 25.0%
! AACATCATGTTATAGATCAT+TGG - tig0019249:160770-160789 None:intergenic 25.0%
! ATTAAATATGTTTGTCTGCT+AGG + tig0019249:160972-160991 Msa1371820:intron 25.0%
!!! GTTTTTGAGAAAACAATGAA+GGG - tig0019249:161066-161085 None:intergenic 25.0%
! TCATGAACTGAAATTACATT+GGG + tig0019249:161343-161362 Msa1371820:intron 25.0%
! ATGAAATAATGAATGTAGTG+TGG + tig0019249:161395-161414 Msa1371820:intron 25.0%
!! TTTCAAGAGTTGATTGATTA+TGG - tig0019249:161582-161601 None:intergenic 25.0%
! ACAATACCATAATAATGTAG+AGG - tig0019249:161743-161762 None:intergenic 25.0%
TGACCCTTGTATATATTTGA+TGG - tig0019249:159519-159538 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG + tig0019249:159864-159883 Msa1371820:CDS 30.0%
ATACCAGTGGATGTTATAAT+TGG - tig0019249:159870-159889 None:intergenic 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG + tig0019249:160523-160542 Msa1371820:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG + tig0019249:160635-160654 Msa1371820:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG - tig0019249:161067-161086 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG + tig0019249:161077-161096 Msa1371820:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG + tig0019249:161281-161300 Msa1371820:intron 30.0%
CTCATGAACTGAAATTACAT+TGG + tig0019249:161342-161361 Msa1371820:intron 30.0%
! ATTTTCTCTTCACTACTCTT+TGG - tig0019249:161472-161491 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG - tig0019249:161646-161665 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG - tig0019249:161790-161809 None:intergenic 30.0%
TGACCCTTGTATATATTTGA+TGG - tig0019249:159519-159538 None:intergenic 30.0%
AATCCAATTATAACATCCAC+TGG + tig0019249:159864-159883 Msa1371820:CDS 30.0%
ATACCAGTGGATGTTATAAT+TGG - tig0019249:159870-159889 None:intergenic 30.0%
!! GGTTTTATAAGGTTCAAATC+AGG + tig0019249:160523-160542 Msa1371820:intron 30.0%
ATAGTCTGGTATGATATGTT+AGG + tig0019249:160635-160654 Msa1371820:intron 30.0%
!! GGTTTTTGAGAAAACAATGA+AGG - tig0019249:161067-161086 None:intergenic 30.0%
! CTCAAAAACCACACATTTTT+CGG + tig0019249:161077-161096 Msa1371820:intron 30.0%
ATGTAGACTGTTTGTTTGAA+TGG + tig0019249:161281-161300 Msa1371820:intron 30.0%
CTCATGAACTGAAATTACAT+TGG + tig0019249:161342-161361 Msa1371820:intron 30.0%
! ATTTTCTCTTCACTACTCTT+TGG - tig0019249:161472-161491 None:intergenic 30.0%
!!! GATCCTTGATCTGAATTTTT+TGG - tig0019249:161646-161665 None:intergenic 30.0%
ATATACTACAAAGGTTGACA+AGG - tig0019249:161790-161809 None:intergenic 30.0%
! AGATCAAACACTTTTCGATC+AGG - tig0019249:159791-159810 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG + tig0019249:159803-159822 Msa1371820:CDS 35.0%
CCAATTGGTTAACAATCAAG+TGG - tig0019249:160171-160190 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG + tig0019249:160168-160187 Msa1371820:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG + tig0019249:160176-160195 Msa1371820:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG + tig0019249:160177-160196 Msa1371820:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG - tig0019249:160358-160377 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG + tig0019249:160425-160444 Msa1371820:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG - tig0019249:160458-160477 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG - tig0019249:160459-160478 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG + tig0019249:160456-160475 Msa1371820:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG - tig0019249:160460-160479 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG + tig0019249:161081-161100 Msa1371820:intron 35.0%
TAGATCTACTCATCTAGACA+AGG - tig0019249:161137-161156 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG + tig0019249:161139-161158 Msa1371820:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG + tig0019249:161232-161251 Msa1371820:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG - tig0019249:161457-161476 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG + tig0019249:161522-161541 Msa1371820:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG - tig0019249:161618-161637 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG - tig0019249:161642-161661 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG + tig0019249:161640-161659 Msa1371820:three_prime_UTR 35.0%
GTTGCTCCTCTACATTATTA+TGG + tig0019249:161734-161753 Msa1371820:three_prime_UTR 35.0%
! AGATCAAACACTTTTCGATC+AGG - tig0019249:159791-159810 None:intergenic 35.0%
TTTGATCTTTCACATGACCA+AGG + tig0019249:159803-159822 Msa1371820:CDS 35.0%
CCAATTGGTTAACAATCAAG+TGG - tig0019249:160171-160190 None:intergenic 35.0%
CCACTTGATTGTTAACCAAT+TGG + tig0019249:160168-160187 Msa1371820:intron 35.0%
!! TTGTTAACCAATTGGTGTCA+TGG + tig0019249:160176-160195 Msa1371820:intron 35.0%
!! TGTTAACCAATTGGTGTCAT+GGG + tig0019249:160177-160196 Msa1371820:intron 35.0%
!! GAACTATATATCATTGGCTC+TGG - tig0019249:160358-160377 None:intergenic 35.0%
TCTACAAATTTCAACATCGC+CGG + tig0019249:160425-160444 Msa1371820:intron 35.0%
!!! CCATATGCATGTTTTGTTTG+GGG - tig0019249:160458-160477 None:intergenic 35.0%
!!! CCCATATGCATGTTTTGTTT+GGG - tig0019249:160459-160478 None:intergenic 35.0%
CCCAAACAAAACATGCATAT+GGG + tig0019249:160456-160475 Msa1371820:intron 35.0%
!! ACCCATATGCATGTTTTGTT+TGG - tig0019249:160460-160479 None:intergenic 35.0%
!! AAAACCACACATTTTTCGGA+TGG + tig0019249:161081-161100 Msa1371820:intron 35.0%
TAGATCTACTCATCTAGACA+AGG - tig0019249:161137-161156 None:intergenic 35.0%
TCTAGATGAGTAGATCTAGA+CGG + tig0019249:161139-161158 Msa1371820:intron 35.0%
ATTAGGTCATAGATCTCTGT+CGG + tig0019249:161232-161251 Msa1371820:intron 35.0%
! CTCTTTGGCTTCATGTTATA+TGG - tig0019249:161457-161476 None:intergenic 35.0%
AAAGAAGATGATCTCAGCTT+CGG + tig0019249:161522-161541 Msa1371820:CDS 35.0%
TGCATGGTAAGTACCTAAAT+TGG - tig0019249:161618-161637 None:intergenic 35.0%
!!! CTTGATCTGAATTTTTTGGC+TGG - tig0019249:161642-161661 None:intergenic 35.0%
CAGCCAAAAAATTCAGATCA+AGG + tig0019249:161640-161659 Msa1371820:three_prime_UTR 35.0%
GTTGCTCCTCTACATTATTA+TGG + tig0019249:161734-161753 Msa1371820:three_prime_UTR 35.0%
TTCGGCTTCATACATTCACT+TGG + tig0019249:159629-159648 Msa1371820:CDS 40.0%
! CATGTCTAGAGCTAACTTAG+AGG + tig0019249:160381-160400 Msa1371820:intron 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG - tig0019249:160447-160466 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG + tig0019249:160455-160474 Msa1371820:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG + tig0019249:160477-160496 Msa1371820:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG + tig0019249:160478-160497 Msa1371820:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG + tig0019249:160488-160507 Msa1371820:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG + tig0019249:160512-160531 Msa1371820:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG - tig0019249:161088-161107 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG + tig0019249:161140-161159 Msa1371820:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG + tig0019249:161254-161273 Msa1371820:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG + tig0019249:161411-161430 Msa1371820:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG + tig0019249:161420-161439 Msa1371820:CDS 40.0%
TGAAGAGAAAATGATGTCCC+AGG + tig0019249:161480-161499 Msa1371820:CDS 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG - tig0019249:161634-161653 None:intergenic 40.0%
TTCGGCTTCATACATTCACT+TGG + tig0019249:159629-159648 Msa1371820:CDS 40.0%
! CATGTCTAGAGCTAACTTAG+AGG + tig0019249:160381-160400 Msa1371820:intron 40.0%
!! TTTTGTTTGGGGAAAAGAGC+CGG - tig0019249:160447-160466 None:intergenic 40.0%
CCCCAAACAAAACATGCATA+TGG + tig0019249:160455-160474 Msa1371820:intron 40.0%
GGTGTGTAGCATTTCAACTA+CGG + tig0019249:160477-160496 Msa1371820:intron 40.0%
GTGTGTAGCATTTCAACTAC+GGG + tig0019249:160478-160497 Msa1371820:intron 40.0%
TTTCAACTACGGGTAGGAAT+AGG + tig0019249:160488-160507 Msa1371820:intron 40.0%
!! TGCGCGATCTAGGTTTTATA+AGG + tig0019249:160512-160531 Msa1371820:intron 40.0%
TTGTCCATCCGAAAAATGTG+TGG - tig0019249:161088-161107 None:intergenic 40.0%
CTAGATGAGTAGATCTAGAC+GGG + tig0019249:161140-161159 Msa1371820:intron 40.0%
GTCGGTCTAACTATTCTCAT+AGG + tig0019249:161254-161273 Msa1371820:intron 40.0%
AGTGTGGAATGAGTTAGAGA+AGG + tig0019249:161411-161430 Msa1371820:intron 40.0%
TGAGTTAGAGAAGGTGAACA+AGG + tig0019249:161420-161439 Msa1371820:CDS 40.0%
TGAAGAGAAAATGATGTCCC+AGG + tig0019249:161480-161499 Msa1371820:CDS 40.0%
!!! GAATTTTTTGGCTGGTTGCA+TGG - tig0019249:161634-161653 None:intergenic 40.0%
TGCAATACCAAGAGCTAGCA+TGG + tig0019249:159593-159612 Msa1371820:exon 45.0%
GCAATACCAAGAGCTAGCAT+GGG + tig0019249:159594-159613 Msa1371820:exon 45.0%
CAATACCAAGAGCTAGCATG+GGG + tig0019249:159595-159614 Msa1371820:exon 45.0%
AATACCAAGAGCTAGCATGG+GGG + tig0019249:159596-159615 Msa1371820:exon 45.0%
CATGACCAAGGAAGAATGCA+TGG + tig0019249:159815-159834 Msa1371820:CDS 45.0%
GCGTGCATGCTTAAGAAGAT+AGG + tig0019249:160204-160223 Msa1371820:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG + tig0019249:160482-160501 Msa1371820:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG + tig0019249:161145-161164 Msa1371820:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG - tig0019249:161500-161519 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG - tig0019249:161501-161520 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG + tig0019249:161535-161554 Msa1371820:CDS 45.0%
TGCAATACCAAGAGCTAGCA+TGG + tig0019249:159593-159612 Msa1371820:exon 45.0%
GCAATACCAAGAGCTAGCAT+GGG + tig0019249:159594-159613 Msa1371820:exon 45.0%
CAATACCAAGAGCTAGCATG+GGG + tig0019249:159595-159614 Msa1371820:exon 45.0%
AATACCAAGAGCTAGCATGG+GGG + tig0019249:159596-159615 Msa1371820:exon 45.0%
CATGACCAAGGAAGAATGCA+TGG + tig0019249:159815-159834 Msa1371820:CDS 45.0%
GCGTGCATGCTTAAGAAGAT+AGG + tig0019249:160204-160223 Msa1371820:intron 45.0%
GTAGCATTTCAACTACGGGT+AGG + tig0019249:160482-160501 Msa1371820:intron 45.0%
TGAGTAGATCTAGACGGGAT+TGG + tig0019249:161145-161164 Msa1371820:intron 45.0%
CATCTGAGTTGTCTCTTCCT+GGG - tig0019249:161500-161519 None:intergenic 45.0%
ACATCTGAGTTGTCTCTTCC+TGG - tig0019249:161501-161520 None:intergenic 45.0%
TCAGCTTCGGATTCCTCTAA+AGG + tig0019249:161535-161554 Msa1371820:CDS 45.0%
CGCTTCCATGCATTCTTCCT+TGG - tig0019249:159823-159842 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG - tig0019249:160186-160205 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG + tig0019249:161236-161255 Msa1371820:intron 50.0%
CACACACGCATGCTAGTGTT+TGG - tig0019249:161315-161334 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG - tig0019249:161551-161570 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG - tig0019249:161693-161712 None:intergenic 50.0%
CGCTTCCATGCATTCTTCCT+TGG - tig0019249:159823-159842 None:intergenic 50.0%
GCATCTCCCATGACACCAAT+TGG - tig0019249:160186-160205 None:intergenic 50.0%
GGTCATAGATCTCTGTCGGT+CGG + tig0019249:161236-161255 Msa1371820:intron 50.0%
CACACACGCATGCTAGTGTT+TGG - tig0019249:161315-161334 None:intergenic 50.0%
GTCGTGTTGTGCACCTTTAG+AGG - tig0019249:161551-161570 None:intergenic 50.0%
GCGCATAGACTACTAGCACT+AGG - tig0019249:161693-161712 None:intergenic 50.0%
CATGGGGGACTCGTCTTCTT+CGG + tig0019249:159611-159630 Msa1371820:exon 55.0%
AGGAATAGGCTGCGCGATCT+AGG + tig0019249:160502-160521 Msa1371820:intron 55.0%
CATGGGGGACTCGTCTTCTT+CGG + tig0019249:159611-159630 Msa1371820:exon 55.0%
AGGAATAGGCTGCGCGATCT+AGG + tig0019249:160502-160521 Msa1371820:intron 55.0%
! GAGTCCCCCATGCTAGCTCT+TGG - tig0019249:159603-159622 None:intergenic 60.0%
! GAGTCCCCCATGCTAGCTCT+TGG - tig0019249:159603-159622 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0019249 gene 159509 161847 159509 ID=Msa1371820;Name=Msa1371820
tig0019249 mRNA 159509 161847 159509 ID=Msa1371820-mRNA-1;Parent=Msa1371820;Name=Msa1371820-mRNA-1;_AED=0.09;_eAED=0.09;_QI=103|1|1|1|0|0|3|271|102
tig0019249 exon 159509 159650 159509 ID=Msa1371820-mRNA-1:exon:2366;Parent=Msa1371820-mRNA-1
tig0019249 exon 159780 159885 159780 ID=Msa1371820-mRNA-1:exon:2367;Parent=Msa1371820-mRNA-1
tig0019249 exon 161413 161847 161413 ID=Msa1371820-mRNA-1:exon:2368;Parent=Msa1371820-mRNA-1
tig0019249 five_prime_UTR 159509 159611 159509 ID=Msa1371820-mRNA-1:five_prime_utr;Parent=Msa1371820-mRNA-1
tig0019249 CDS 159612 159650 159612 ID=Msa1371820-mRNA-1:cds;Parent=Msa1371820-mRNA-1
tig0019249 CDS 159780 159885 159780 ID=Msa1371820-mRNA-1:cds;Parent=Msa1371820-mRNA-1
tig0019249 CDS 161413 161576 161413 ID=Msa1371820-mRNA-1:cds;Parent=Msa1371820-mRNA-1
tig0019249 three_prime_UTR 161577 161847 161577 ID=Msa1371820-mRNA-1:three_prime_utr;Parent=Msa1371820-mRNA-1
Gene Sequence

>Msa1371820

ATGGGGGACTCGTCTTCTTCGGCTTCATACATTCACTTGGTGCAGCACCTGATCGAAAAGTGTTTGATCTTTCACATGACCAAGGAAGAATGCATGGAAGCGCTCTCCAAACATGCAAATATAAATCCAATTATAACATCCACTGTGTGGAATGAGTTAGAGAAGGTGAACAAGGAATTTTTTGAGCCATATAACATGAAGCCAAAGAGTAGTGAAGAGAAAATGATGTCCCAGGAAGAGACAACTCAGATGTTAAAGAAGATGATCTCAGCTTCGGATTCCTCTAAAGGTGCACAACACGACGACTAA
Protein sequence

>Msa1371820

MGDSSSSASYIHLVQHLIEKCLIFHMTKEECMEALSKHANINPIITSTVWNELEKVNKEFFEPYNMKPKSSEEKMMSQEETTQMLKKMISASDSSKGAQHDD*