AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0078530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078530 MtrunA17_Chr1g0189171 94.608 1020 26 2 1 999 1 1012 0.0 1699
Msa0078530 MtrunA17_Chr1g0189201 58.003 631 166 21 6 609 9 567 4.91e-155 470
Msa0078530 MtrunA17_Chr1g0189181 65.027 183 55 3 304 479 32 212 1.46e-56 194
Msa0078530 MtrunA17_Chr1g0189181 49.761 209 75 8 2 202 28 214 8.58e-32 124
Msa0078530 MtrunA17_Chr1g0189181 88.235 34 4 0 623 656 1 34 2.32e-11 64.7
Msa0078530 MtrunA17_Chr5g0447931 48.052 154 72 3 832 984 37 183 3.03e-31 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078530 AT1G10390.3 57.328 1078 332 35 1 992 1 1036 0.0 966
Msa0078530 AT1G10390.1 57.328 1078 332 35 1 992 1 1036 0.0 966
Msa0078530 AT1G10390.2 57.328 1078 332 35 1 992 1 1036 0.0 966
Msa0078530 AT1G59660.1 52.367 1056 376 34 1 991 1 994 0.0 810
Msa0078530 AT1G59660.3 51.989 1056 373 34 1 991 1 987 0.0 801
Msa0078530 AT1G59660.2 51.989 1056 373 34 1 991 1 987 0.0 801
Msa0078530 AT1G80680.1 47.126 174 85 3 820 993 31 197 8.13e-37 150

Find 351 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTCTTATATTTCCTTAT+AGG 0.139481 1_2:+56124985 None:intergenic
GGTCAGAATACCTCCCAATT+TGG 0.174042 1_2:-56126695 Msa0078530:CDS
TGAAAAGCTTACAGTGAAAT+TGG 0.174060 1_2:+56130148 None:intergenic
GAACTTCCATTCTCCTTTAT+TGG 0.188439 1_2:+56122928 None:intergenic
AAGCACCAGTTTGCCCAAAT+TGG 0.194534 1_2:+56126681 None:intergenic
GCATTTGGAAGCAGCATATT+TGG 0.195207 1_2:-56128031 Msa0078530:CDS
GCAACCAGCACCCCAGCTTT+TGG 0.199572 1_2:-56127842 Msa0078530:CDS
TCTTTATATTTCCCGATTCT+TGG 0.211348 1_2:+56123004 None:intergenic
GCCTCCAGCTCTCCTGCTTT+TGG 0.214621 1_2:-56127659 Msa0078530:CDS
CATCAAGCACGCCTGCATTT+GGG 0.234663 1_2:-56127682 Msa0078530:CDS
GGGAGTACGACCTCTAATTT+TGG 0.244346 1_2:-56127548 Msa0078530:CDS
GACAATACATTTCATAGTTT+TGG 0.244724 1_2:-56122801 Msa0078530:three_prime_UTR
GGTACTTCCACCGGTGTATT+TGG 0.252477 1_2:-56129413 Msa0078530:CDS
GTGCTGGTTGCACCGAAAGC+TGG 0.262885 1_2:+56127854 None:intergenic
TGTGCAAATGGGTTAGAATT+AGG 0.267243 1_2:+56126977 None:intergenic
AACTTCCATTCTCCTTTATT+GGG 0.268732 1_2:+56122929 None:intergenic
GTGCTGGTTGCCCCAAAAGC+TGG 0.268797 1_2:+56127830 None:intergenic
GTACTTCAATGCCGGCATTT+GGG 0.272314 1_2:-56129325 Msa0078530:CDS
GCTTCAAGCAGTCCTGTATT+TGG 0.277522 1_2:-56127707 Msa0078530:CDS
TCTAATGCTCCGACAGGTTT+TGG 0.279078 1_2:-56126590 Msa0078530:CDS
TCATCAAGCACGCCTGCATT+TGG 0.282285 1_2:-56127683 Msa0078530:CDS
TTTGGTCAGAAGCCTTCTTT+TGG 0.286295 1_2:-56128151 Msa0078530:CDS
GCACCCAGCGCCCCAGCTTT+CGG 0.287640 1_2:-56127866 Msa0078530:CDS
TAGGGGCAGAAGTGTTAAAT+AGG 0.292855 1_2:+56126738 None:intergenic
TGCTGGTTGCCCCAAAAGCT+GGG 0.297700 1_2:+56127831 None:intergenic
CACCCGCAACTGCTCCGTTT+GGG 0.300844 1_2:-56126865 Msa0078530:CDS
TGCTGGTTGCACCGAAAGCT+GGG 0.302073 1_2:+56127855 None:intergenic
GGTACTTCAATGCCGGCATT+TGG 0.303504 1_2:-56129326 Msa0078530:CDS
GGTATTGGGCAGTCAGGATT+TGG 0.303970 1_2:-56127374 Msa0078530:CDS
TCCTCTCCCATATTGTTATA+TGG 0.306591 1_2:+56122825 None:intergenic
GCTTCATCATCTCCAGCTTT+TGG 0.308542 1_2:-56129347 Msa0078530:CDS
ACAAATGCACAGTCTGGTTT+TGG 0.309202 1_2:-56126716 Msa0078530:CDS
GCAACCAGCACCCCTGCATT+TGG 0.309240 1_2:-56127818 Msa0078530:CDS
GCACCCAGCACCCCTGCTTT+TGG 0.309345 1_2:-56127890 Msa0078530:CDS
ACATGTCCCATATAACAATA+TGG 0.312202 1_2:-56122832 Msa0078530:three_prime_UTR
ACCACTGTGTTTGGTGGTGG+TGG 0.317969 1_2:-56127740 Msa0078530:CDS
GCAACCAGCACCCCTGCTTT+TGG 0.319005 1_2:-56127914 Msa0078530:CDS
TTAGGGACTGCAGCTGAAAA+TGG 0.325573 1_2:-56123523 Msa0078530:intron
GGCTCGTCTCAATCCGTGTT+CGG 0.334608 1_2:-56129533 Msa0078530:CDS
CCATTTGCACAAAAGACATT+TGG 0.344011 1_2:-56126965 Msa0078530:CDS
ACCTCCCAATTTGGGCAAAC+TGG 0.346514 1_2:-56126686 Msa0078530:CDS
GTCAGAATACCTCCCAATTT+GGG 0.347524 1_2:-56126694 Msa0078530:CDS
TGGAAAGTTGTAAGCATGTT+TGG 0.348227 1_2:-56129903 Msa0078530:exon
GCTTTCTCAGAAGTCCTTCC+AGG 0.348991 1_2:+56124757 None:intergenic
TGCACCGAAAGCTGGGGCGC+TGG 0.349468 1_2:+56127862 None:intergenic
TGCACCAAAAGCAGGGGTGC+TGG 0.350019 1_2:+56127886 None:intergenic
ACACCCGCAACTGCTCCGTT+TGG 0.350724 1_2:-56126866 Msa0078530:CDS
AGTCAGTCTAGTTCTGCATT+TGG 0.351850 1_2:-56127587 Msa0078530:CDS
TCAACTTCAACCTCAGCTTT+TGG 0.352834 1_2:-56127794 Msa0078530:CDS
GAAGAGTCAAGTTTCCTATA+AGG 0.352898 1_2:-56124999 Msa0078530:CDS
GACTATCAATTGGGAGATAA+AGG 0.353749 1_2:-56127206 Msa0078530:intron
GTCAGGATTTGGAGGTCAAC+GGG 0.354139 1_2:-56127363 Msa0078530:CDS
ACCACTCCTTCCGTTCAATA+TGG 0.354990 1_2:-56125283 Msa0078530:CDS
GCATCTTCAACTCCGGCTTT+TGG 0.355204 1_2:-56129302 Msa0078530:CDS
GCTGGTGCAAGTAGCTTTGC+TGG 0.355697 1_2:-56125658 Msa0078530:CDS
GAAAAGCTTACAGTGAAATT+GGG 0.358735 1_2:+56130149 None:intergenic
GGCTATTCCCCAGAGTTCTA+TGG 0.359916 1_2:-56125383 Msa0078530:CDS
GGATACAAGCTGATGTATAA+AGG 0.363721 1_2:-56122859 Msa0078530:exon
TTTGGCACCAGCAGCCCATT+TGG 0.372926 1_2:-56127569 Msa0078530:CDS
TCGACAGGCACTCCTGCATT+TGG 0.377919 1_2:-56127986 Msa0078530:CDS
GCCCCAAACGGAGCAGTTGC+GGG 0.378209 1_2:+56126863 None:intergenic
GCATTTGGAAGTACTGGTAT+TGG 0.384090 1_2:-56127389 Msa0078530:CDS
ATAAAGGAGAATGGAAGTTC+AGG 0.385079 1_2:-56122925 Msa0078530:CDS
CTCTGGACAGGCTGCAAAGT+TGG 0.388026 1_2:-56127288 Msa0078530:CDS
AAATATATCCCAAAGCAAAA+AGG 0.388835 1_2:+56122681 None:intergenic
GCGCTGGGTGCACCAAAAGC+AGG 0.389384 1_2:+56127878 None:intergenic
TTATTCAGCCCTTGTCCAAC+AGG 0.390186 1_2:+56123097 None:intergenic
TTTATAGGATCATCAGTGTT+TGG 0.391041 1_2:-56128169 Msa0078530:intron
GTGCTGGGTGCACCAAAAGC+AGG 0.391403 1_2:+56127902 None:intergenic
CAAACGGGTAATTCCGTGTT+TGG 0.392079 1_2:-56129437 Msa0078530:CDS
CATTTGGAAGTACTGGTATT+GGG 0.392197 1_2:-56127388 Msa0078530:CDS
TAGATCTGCGAGAAGAGATT+AGG 0.395385 1_2:+56130177 None:intergenic
TACAGTAGCAGGTTTGTCTT+GGG 0.396511 1_2:+56125062 None:intergenic
CACTGTGTTTGGTGGTGGTG+GGG 0.397049 1_2:-56127738 Msa0078530:CDS
GCTCGTCTCAATCCGTGTTC+GGG 0.397437 1_2:-56129532 Msa0078530:CDS
TTGGCACCAGCAGCCCATTT+GGG 0.397862 1_2:-56127568 Msa0078530:CDS
TTTGGGAGTCCAACTCCATT+TGG 0.398461 1_2:-56129467 Msa0078530:CDS
CAACCATCACAACCAGCATT+TGG 0.399302 1_2:-56128079 Msa0078530:CDS
ACTTTGACCTCCAAAATTAG+AGG 0.401833 1_2:+56127538 None:intergenic
CAACAGTCGCAATCAGCATT+TGG 0.403158 1_2:-56128046 Msa0078530:CDS
GGTTCCTCAACACCAGCATT+TGG 0.403378 1_2:-56128010 Msa0078530:CDS
GGTAATTCGTCATCGTCATT+TGG 0.404398 1_2:-56129281 Msa0078530:CDS
GGAGGTCAAAGTTCTGCATT+TGG 0.406400 1_2:-56127527 Msa0078530:intron
CAGACTCCCACTCAAGCATT+TGG 0.409112 1_2:-56127404 Msa0078530:CDS
CGGATTGAGACGAGCCGAAC+GGG 0.409832 1_2:+56129540 None:intergenic
AGCACCAGTTTGCCCAAATT+GGG 0.411313 1_2:+56126682 None:intergenic
TTACAGTAGCAGGTTTGTCT+TGG 0.411503 1_2:+56125061 None:intergenic
ATGTGCCTTGACCACCAAAC+AGG 0.411754 1_2:+56126804 None:intergenic
ACTGCCTCGTATTCATGAAT+TGG 0.411963 1_2:-56123302 Msa0078530:CDS
TAATCAGACCGTCGCAGCTT+TGG 0.412110 1_2:+56123331 None:intergenic
CCTACTCAAACAAGTCCATT+TGG 0.414331 1_2:-56128112 Msa0078530:CDS
AACTGGTGAAGTTTAGTGCC+AGG 0.414647 1_2:-56122602 Msa0078530:three_prime_UTR
AAGTGTTAAATAGGCTTGGC+TGG 0.416555 1_2:+56126747 None:intergenic
GCACCGAAAGCTGGGGCGCT+GGG 0.418196 1_2:+56127863 None:intergenic
GCTTTGCCTCAGATGTCAAT+TGG 0.419405 1_2:-56125316 Msa0078530:CDS
ATTGCAGTTGGGGATTATCT+AGG 0.420769 1_2:-56129938 Msa0078530:intron
CAAATGCTGGTTGTGATGGT+TGG 0.422181 1_2:+56128080 None:intergenic
GCACCAAAAGCAGGGGTGCT+GGG 0.422344 1_2:+56127887 None:intergenic
GCCTCTGCTCCTCCGTCAAC+TGG 0.423618 1_2:-56127082 Msa0078530:CDS
CAAATGCTGATTGCGACTGT+TGG 0.423921 1_2:+56128047 None:intergenic
TGTCCCATGGAGCAGTGGCC+TGG 0.426491 1_2:-56124775 Msa0078530:CDS
CCTCTCCCATATTGTTATAT+GGG 0.426931 1_2:+56122826 None:intergenic
CCACTGTGTTTGGTGGTGGT+GGG 0.427431 1_2:-56127739 Msa0078530:CDS
AACCAGACTGTGCATTTGTA+GGG 0.430698 1_2:+56126720 None:intergenic
TACACTGAAGGACCAAGAAT+CGG 0.431501 1_2:-56123016 Msa0078530:CDS
TGCCCCAAAAGCTGGGGTGC+TGG 0.432347 1_2:+56127838 None:intergenic
TTTGGTGCTTCTAGTGCCCC+AGG 0.432998 1_2:-56127641 Msa0078530:CDS
GCTACGAGCACTCCTGCATT+TGG 0.435030 1_2:-56127962 Msa0078530:CDS
GTTCCAAATGGGTTTGTAAT+GGG 0.435848 1_2:+56125346 None:intergenic
AAGATGGGAGGACTATCAAT+TGG 0.435890 1_2:-56127216 Msa0078530:CDS
GCTACGAGCACCCCTGCGTT+TGG 0.438383 1_2:-56127938 Msa0078530:CDS
GCACCCATTACAAACCCATT+TGG 0.439385 1_2:-56125349 Msa0078530:CDS
GAGCTGGAGGCCCCAAATGC+AGG 0.441051 1_2:+56127671 None:intergenic
ACTGTGTTTGGTGGTGGTGG+GGG 0.444013 1_2:-56127737 Msa0078530:CDS
AGATCTGCGAGAAGAGATTA+GGG 0.444087 1_2:+56130178 None:intergenic
GGCCCCAAACGGAGCAGTTG+CGG 0.444928 1_2:+56126862 None:intergenic
TGCACCAAAAGCAGGGGTGC+TGG 0.445260 1_2:+56127910 None:intergenic
ATCTACACCTTCTTTCCAAT+CGG 0.446617 1_2:-56125828 Msa0078530:CDS
GTATCATATGATCCCAATAA+AGG 0.449462 1_2:-56122941 Msa0078530:CDS
CTTGTGCAACCATAGAACTC+TGG 0.449744 1_2:+56125374 None:intergenic
GCATCAGCCTTTGGTGTGGT+TGG 0.450225 1_2:+56124838 None:intergenic
AGCACCAAAAGCAGGAGAGC+TGG 0.451061 1_2:+56127655 None:intergenic
GGGTCATCTACATCCCTGTT+TGG 0.451560 1_2:-56126818 Msa0078530:CDS
TGAACTAACCAGGTTGTGTC+TGG 0.452741 1_2:+56125777 None:intergenic
ATTTGGGGCATCTTCAACTC+CGG 0.452768 1_2:-56129309 Msa0078530:CDS
ATGAAAAGGGCATCAGCCTT+TGG 0.454264 1_2:+56124829 None:intergenic
AAACCAGACTGTGCATTTGT+AGG 0.456191 1_2:+56126719 None:intergenic
CCAAATGGCTTAGGTGCAAA+AGG 0.459149 1_2:+56129485 None:intergenic
GATACAAGCTGATGTATAAA+GGG 0.460606 1_2:-56122858 Msa0078530:exon
AAATGCTGGTTGTGATGGTT+GGG 0.460765 1_2:+56128081 None:intergenic
TTGAGGAGTGTTACCTCAGC+AGG 0.461242 1_2:+56123073 None:intergenic
TGCTGGTTGCACCAAACGCA+GGG 0.461264 1_2:+56127927 None:intergenic
ATGGTCTTCGTTATTCCCAT+TGG 0.461838 1_2:+56123453 None:intergenic
TCCATTTGGATCGCAGCAAA+CGG 0.462848 1_2:-56129453 Msa0078530:CDS
GCTCAAACTCAAAGCATGTT+TGG 0.463757 1_2:-56125634 Msa0078530:CDS
GGGCAGTCATCGAGCCCGTT+CGG 0.464084 1_2:-56129554 Msa0078530:CDS
CTTCCAGGCCACTGCTCCAT+GGG 0.464292 1_2:+56124772 None:intergenic
GAAGCAGATAGTGGCACCTC+TGG 0.465746 1_2:-56127305 Msa0078530:CDS
CTTCCAAATGCTGGTTGTGA+TGG 0.465773 1_2:+56128076 None:intergenic
GCCCCTACAAATGCACAGTC+TGG 0.468736 1_2:-56126722 Msa0078530:CDS
AGTACTGGTATTGGGCAGTC+AGG 0.469117 1_2:-56127380 Msa0078530:CDS
AAAGGACATATCAACAAGAT+TGG 0.470029 1_2:+56122699 None:intergenic
CCCGTTTGCTGCGATCCAAA+TGG 0.472542 1_2:+56129452 None:intergenic
CCTTCCAGGCCACTGCTCCA+TGG 0.473071 1_2:+56124771 None:intergenic
TTAGAGGTCGTACTCCCAAA+TGG 0.473073 1_2:+56127554 None:intergenic
GAGGATATTCTTACAGTAGC+AGG 0.473971 1_2:+56125051 None:intergenic
ACCCGCAACTGCTCCGTTTG+GGG 0.474562 1_2:-56126864 Msa0078530:CDS
TGATGAAGCGCCGAAAGTAT+TGG 0.475170 1_2:+56129361 None:intergenic
CGAAGACCATTCTCTGCAAA+AGG 0.475245 1_2:-56123440 Msa0078530:CDS
CGCTGGGTGCACCAAAAGCA+GGG 0.478449 1_2:+56127879 None:intergenic
GCCAAAGTTGCTAAAGGAAA+AGG 0.480874 1_2:+56125799 None:intergenic
ATTGGAAAGAAGGTGTAGAT+TGG 0.481485 1_2:+56125831 None:intergenic
TACTTCAATGCCGGCATTTG+GGG 0.482872 1_2:-56129324 Msa0078530:CDS
TTGACATGACTGCAGAATCC+TGG 0.488318 1_2:+56123259 None:intergenic
ACTCAAGCATTTGGAAGTAC+TGG 0.489144 1_2:-56127395 Msa0078530:CDS
CCTCCGTCAACTGGTATGCC+AGG 0.491081 1_2:-56127073 Msa0078530:CDS
AAAGCAGGAAGTGTTCCAAA+TGG 0.491487 1_2:+56125334 None:intergenic
GACTTTGTCGTTGGAAGGCA+AGG 0.491709 1_2:-56123235 Msa0078530:CDS
GCCTCGTATTCATGAATTGG+CGG 0.491804 1_2:-56123299 Msa0078530:CDS
TGGGTGTTACTTCCAAATGC+TGG 0.491830 1_2:+56128067 None:intergenic
AAAGCCACGAGGAGCTAAGA+TGG 0.492465 1_2:-56127232 Msa0078530:CDS
ATGTTCAATCAACCTTCATC+TGG 0.492928 1_2:-56126644 Msa0078530:CDS
GTACCAGCCGATTGGAAAGA+AGG 0.493122 1_2:+56125821 None:intergenic
TGTTCCAAATGGGTTTGTAA+TGG 0.494999 1_2:+56125345 None:intergenic
ATGATCCCAATAAAGGAGAA+TGG 0.495773 1_2:-56122934 Msa0078530:CDS
GTCGTTGGAAGGCAAGGTTA+TGG 0.496990 1_2:-56123229 Msa0078530:CDS
TATTCATGAATTGGCGGCTA+AGG 0.497209 1_2:-56123293 Msa0078530:CDS
GTTGAAGATGCCCCAAATGC+CGG 0.498443 1_2:+56129314 None:intergenic
GTTGGACTCCCAAATGGCTT+AGG 0.498646 1_2:+56129476 None:intergenic
TGCTGGGTGCACCAAAAGCA+GGG 0.498893 1_2:+56127903 None:intergenic
ATCAAGCACGCCTGCATTTG+GGG 0.499047 1_2:-56127681 Msa0078530:CDS
TAAAGGAGAATGGAAGTTCA+GGG 0.500263 1_2:-56122924 Msa0078530:CDS
GACGAGGAGTTGGCCCCAAA+CGG 0.500738 1_2:+56126851 None:intergenic
GGCATTGAAGTACCAAAAGC+TGG 0.504883 1_2:+56129335 None:intergenic
GTGTTTGGTGGTACTTCCAC+CGG 0.505032 1_2:-56129422 Msa0078530:CDS
AGATAGTGGCACCTCTGGAC+AGG 0.505057 1_2:-56127300 Msa0078530:CDS
GGTGTTGTGCTACTAGAGAA+AGG 0.505152 1_2:+56126998 None:intergenic
CCATGGAGCAGTGGCCTGGA+AGG 0.506677 1_2:-56124771 Msa0078530:CDS
CCATTTGGATCGCAGCAAAC+GGG 0.507092 1_2:-56129452 Msa0078530:CDS
ACTCCTCGTCACAGCCAGTT+GGG 0.507517 1_2:-56126838 Msa0078530:CDS
ATTGACATCTGAGGCAAAGC+AGG 0.507708 1_2:+56125319 None:intergenic
ACACTGAAGGACCAAGAATC+GGG 0.507714 1_2:-56123015 Msa0078530:CDS
AAGCAGGAAGTGTTCCAAAT+GGG 0.508299 1_2:+56125335 None:intergenic
ACACCTTCTTTCCAATCGGC+TGG 0.509909 1_2:-56125824 Msa0078530:CDS
GACAGTACCATTCTCATTCA+CGG 0.510075 1_2:+56124708 None:intergenic
GAGAAAAGGCTTCCAGATGA+AGG 0.510564 1_2:+56126632 None:intergenic
AACTTTGGCCAGACACAACC+TGG 0.510599 1_2:-56125785 Msa0078530:intron
TTTATCTTTGTAAACAGGCA+TGG 0.510768 1_2:+56127253 None:intergenic
CGTACTCCCAAATGGGCTGC+TGG 0.511093 1_2:+56127562 None:intergenic
CATGTCCCATATAACAATAT+GGG 0.511531 1_2:-56122831 Msa0078530:three_prime_UTR
GGACAAATGATCAACGCCCT+TGG 0.512561 1_2:+56124793 None:intergenic
TATAAGGAAATATAAGAATG+AGG 0.513013 1_2:-56124983 Msa0078530:CDS
GATGATGAAGAAGGAAATGC+TGG 0.513464 1_2:+56126917 None:intergenic
ATGTCAAATACCACTGTGTT+TGG 0.514225 1_2:-56127749 Msa0078530:CDS
ACATCCCTGTTTGGTGGTCA+AGG 0.514827 1_2:-56126809 Msa0078530:CDS
TAAAGAGATGCTCAAGAGAA+AGG 0.516587 1_2:-56122987 Msa0078530:CDS
GTACGAAGCATGCATCCAAT+GGG 0.517463 1_2:-56123468 Msa0078530:CDS
CCACTTCAGCGTTTACAAGC+TGG 0.517488 1_2:-56122897 Msa0078530:CDS
ACACCAGCATTTGGTTCGAC+AGG 0.518178 1_2:-56128001 Msa0078530:CDS
TTGTGCAACCATAGAACTCT+GGG 0.518512 1_2:+56125375 None:intergenic
CAGATGTCAATTGGTCGAGT+TGG 0.518776 1_2:-56125307 Msa0078530:CDS
TCTTCATCATCACTAAAGAA+TGG 0.520226 1_2:+56124865 None:intergenic
GAGCCAAAACCTCCAAAAGA+AGG 0.520720 1_2:+56128139 None:intergenic
GGCGTTGATCATTTGTCCCA+TGG 0.521200 1_2:-56124788 Msa0078530:CDS
GAAGGTTGATTGAACATGCT+TGG 0.522236 1_2:+56126650 None:intergenic
AAATGCTGATTGCGACTGTT+GGG 0.523958 1_2:+56128048 None:intergenic
CATGTCAAGGACTTTGTCGT+TGG 0.524179 1_2:-56123244 Msa0078530:CDS
TAGAGGTCGTACTCCCAAAT+GGG 0.525993 1_2:+56127555 None:intergenic
CCGAAAGTATTGGAAGAAAA+CGG 0.526469 1_2:+56129371 None:intergenic
TGGAACCAAAACTAAAACCT+GGG 0.526628 1_2:+56127624 None:intergenic
AATGGAGTTGGACTCCCAAA+TGG 0.527280 1_2:+56129470 None:intergenic
TGCTGCGATCCAAATGGAGT+TGG 0.528471 1_2:+56129458 None:intergenic
TCAAGGACTTTGTCGTTGGA+AGG 0.528854 1_2:-56123240 Msa0078530:CDS
GTGCTGGTTGCACCAAACGC+AGG 0.529302 1_2:+56127926 None:intergenic
CCAAATGGACTTGTTTGAGT+AGG 0.529453 1_2:+56128112 None:intergenic
GTCGTAGACGAATTAAAGCC+TGG 0.529750 1_2:+56127055 None:intergenic
CAGTGGGCACAGAGCTGGTG+AGG 0.530438 1_2:-56123398 Msa0078530:CDS
AAGAAACCTCCTGTTGGACA+AGG 0.532888 1_2:-56123106 Msa0078530:CDS
TGAACCAAATGCAGGGGTGC+TGG 0.532978 1_2:+56127814 None:intergenic
TCATTCCTAGGGAAAATCCA+AGG 0.533348 1_2:-56124810 Msa0078530:CDS
TGACTCTTCTTTGTGAGAGG+TGG 0.533352 1_2:+56125013 None:intergenic
GCTACAGTGGAAGCAGATAG+TGG 0.534008 1_2:-56127314 Msa0078530:CDS
GCAGAAGTGTTAAATAGGCT+TGG 0.534605 1_2:+56126743 None:intergenic
TCATCTACATCCCTGTTTGG+TGG 0.536524 1_2:-56126815 Msa0078530:CDS
GATGCATGCTTCGTACGTAC+AGG 0.536543 1_2:+56123475 None:intergenic
TACACCTCCGTGAATGAGAA+TGG 0.536980 1_2:-56124715 Msa0078530:intron
AGGAAAAGGTACCAGCCGAT+TGG 0.537215 1_2:+56125813 None:intergenic
AACTCCTCGTCACAGCCAGT+TGG 0.537472 1_2:-56126839 Msa0078530:CDS
GTTGAAGTTGAACCAAATGC+AGG 0.538366 1_2:+56127806 None:intergenic
AGTCAGGATTTGGAGGTCAA+CGG 0.542037 1_2:-56127364 Msa0078530:CDS
GGTTGGGTGGCACTTCCAAA+TGG 0.543213 1_2:+56128097 None:intergenic
AGCTGGTAGATGAAGACAGT+TGG 0.546871 1_2:-56122880 Msa0078530:CDS
ACTTGACTCTTCTTTGTGAG+AGG 0.547105 1_2:+56125010 None:intergenic
ACCAAAAGCAGGAGAGCTGG+AGG 0.547838 1_2:+56127658 None:intergenic
GTGATGGGCTGCTTGAGAAA+AGG 0.548542 1_2:+56126618 None:intergenic
CGTACGAAGCATGCATCCAA+TGG 0.549638 1_2:-56123469 Msa0078530:CDS
CACTAATGCCAAAGCTGCGA+CGG 0.551023 1_2:-56123339 Msa0078530:CDS
AGATGGGAGGACTATCAATT+GGG 0.551615 1_2:-56127215 Msa0078530:CDS
CTACTAGAGAAAGGATTGGC+TGG 0.552358 1_2:+56127007 None:intergenic
GTGCTCGTAGCACCAAATGC+AGG 0.553367 1_2:+56127974 None:intergenic
ATATAAGAATGAGGGATCAA+AGG 0.554354 1_2:-56124974 Msa0078530:intron
GGTGTATCTATCCTTAAAAG+AGG 0.554469 1_2:+56124732 None:intergenic
GCTGGTTGCCCCAAAAGCTG+GGG 0.556557 1_2:+56127832 None:intergenic
GTGCTTGATGAACCAAATAC+AGG 0.557053 1_2:+56127695 None:intergenic
ACACTCAGTGGGCACAGAGC+TGG 0.558567 1_2:-56123403 Msa0078530:CDS
AACTGGCTGTGACGAGGAGT+TGG 0.560749 1_2:+56126841 None:intergenic
AATACCACTGTGTTTGGTGG+TGG 0.561037 1_2:-56127743 Msa0078530:CDS
GTGGAACCAAAACTAAAACC+TGG 0.562126 1_2:+56127623 None:intergenic
TCATTTGTCCCATGGAGCAG+TGG 0.564174 1_2:-56124780 Msa0078530:CDS
AAGCCTGGCATACCAGTTGA+CGG 0.564463 1_2:+56127070 None:intergenic
GCACTAGAAGCACCAAAAGC+AGG 0.564969 1_2:+56127647 None:intergenic
CATATCAACAAGATTGGCCC+TGG 0.565125 1_2:+56122705 None:intergenic
AGGGATGTAGATGACCCAAC+TGG 0.566062 1_2:+56126824 None:intergenic
TGCTGGTTGTGATGGTTGGG+TGG 0.568361 1_2:+56128084 None:intergenic
GAGGTGGCGAGAAGTCAATA+AGG 0.568451 1_2:+56125029 None:intergenic
CCAGCTTGTAAACGCTGAAG+TGG 0.568812 1_2:+56122897 None:intergenic
TCGTGAGGTGATTGTATACA+TGG 0.570815 1_2:-56123137 Msa0078530:CDS
ATAAGGAAATATAAGAATGA+GGG 0.572214 1_2:-56124982 Msa0078530:CDS
GTTGTTCTGCTGCCCGAACA+CGG 0.574376 1_2:+56129520 None:intergenic
TGTCTGGCCAAAGTTGCTAA+AGG 0.574424 1_2:+56125793 None:intergenic
AATTCGTCATCGTCATTTGG+TGG 0.574431 1_2:-56129278 Msa0078530:intron
CATTCCTAGGGAAAATCCAA+GGG 0.574460 1_2:-56124809 Msa0078530:CDS
AGGAAATGCTGGTGAACTGA+AGG 0.575618 1_2:+56126928 None:intergenic
ATGTTTGGTCAGAATAATGT+TGG 0.578136 1_2:-56125619 Msa0078530:CDS
TTCAGCCCTTGTCCAACAGG+AGG 0.580774 1_2:+56123100 None:intergenic
GAGATACCATATTGAACGGA+AGG 0.584307 1_2:+56125277 None:intergenic
GTGCTCGTAGCACCAAATGC+AGG 0.588399 1_2:+56127950 None:intergenic
AATGCTGGTGAACTGAAGGC+AGG 0.588493 1_2:+56126932 None:intergenic
CCCATATAACAATATGGGAG+AGG 0.588621 1_2:-56122826 Msa0078530:three_prime_UTR
GCTAAGGAAAGAGCTGAACC+AGG 0.589537 1_2:-56123277 Msa0078530:CDS
ACCAGTTGACGGAGGAGCAG+AGG 0.590749 1_2:+56127081 None:intergenic
GATGCTCAAGAGAAAGGCTG+AGG 0.590765 1_2:-56122981 Msa0078530:CDS
GATTCAAAGAAACCTCCTGT+TGG 0.591139 1_2:-56123112 Msa0078530:CDS
ACTGGTGAAGTTTAGTGCCA+GGG 0.591803 1_2:-56122601 Msa0078530:three_prime_UTR
ACGGGTAATTCCGTGTTTGG+TGG 0.591957 1_2:-56129434 Msa0078530:CDS
AAAAGCTTACAGTGAAATTG+GGG 0.592263 1_2:+56130150 None:intergenic
TTGAAGTTGAACCAAATGCA+GGG 0.592879 1_2:+56127807 None:intergenic
ATTGGGCAGTCAGGATTTGG+AGG 0.596861 1_2:-56127371 Msa0078530:CDS
ACCAGTTTGCCCAAATTGGG+AGG 0.598471 1_2:+56126685 None:intergenic
AGTGTTAAATAGGCTTGGCT+GGG 0.599695 1_2:+56126748 None:intergenic
ACCATATTGAACGGAAGGAG+TGG 0.600178 1_2:+56125282 None:intergenic
GCTGGGTGCACCAAAAGCAG+GGG 0.601140 1_2:+56127880 None:intergenic
AGGATTCTGCAGTCATGTCA+AGG 0.601362 1_2:-56123257 Msa0078530:CDS
GAACTAGACTGACTAAAAGC+CGG 0.602042 1_2:+56127596 None:intergenic
GCTGCTGATGATGATGAAGA+AGG 0.605501 1_2:+56126908 None:intergenic
CTCACATCTAATGCTCCGAC+AGG 0.605827 1_2:-56126596 Msa0078530:CDS
GTGCCTGTCGAACCAAATGC+TGG 0.605979 1_2:+56127998 None:intergenic
AAGCCACGAGGAGCTAAGAT+GGG 0.606234 1_2:-56127231 Msa0078530:CDS
GCTGGTTGCACCGAAAGCTG+GGG 0.607847 1_2:+56127856 None:intergenic
GCTGGGTGCACCAAAAGCAG+GGG 0.608639 1_2:+56127904 None:intergenic
GGATTTGGAGGTCAACGGGG+TGG 0.608669 1_2:-56127359 Msa0078530:CDS
AGTACTTCCAAATGCTTGAG+TGG 0.609773 1_2:+56127397 None:intergenic
ACGATTGTTAAACTGAACAA+TGG 0.611463 1_2:+56123156 None:intergenic
CTGACTAAAAGCCGGAGTGG+TGG 0.612070 1_2:+56127604 None:intergenic
GGAGCATTAGATGTGAGTGA+TGG 0.612114 1_2:+56126602 None:intergenic
TCAAATACCACTGTGTTTGG+TGG 0.614427 1_2:-56127746 Msa0078530:CDS
GAGCATTAGATGTGAGTGAT+GGG 0.617858 1_2:+56126603 None:intergenic
CCCTGTGTTACCAAAAGCTG+AGG 0.618001 1_2:+56127784 None:intergenic
ACGGATTGAGACGAGCCGAA+CGG 0.620717 1_2:+56129539 None:intergenic
AGCTAGTTACTCAGCTACAG+TGG 0.621042 1_2:-56127327 Msa0078530:CDS
AAGAGAAAGGCTGAGGACCA+AGG 0.622470 1_2:-56122974 Msa0078530:CDS
TGTTCAGTTTAACAATCGTG+AGG 0.624585 1_2:-56123152 Msa0078530:CDS
ATGATACAAACTCAGCACCT+TGG 0.625305 1_2:+56122957 None:intergenic
GCTAGAGATACCATATTGAA+CGG 0.627623 1_2:+56125273 None:intergenic
AGCTGAAAATGGTGTTGTCA+AGG 0.628588 1_2:-56123512 Msa0078530:CDS
CTGAGTTGCGCCAAATACAC+CGG 0.630009 1_2:+56129403 None:intergenic
GTACTTCCAAATGCTTGAGT+GGG 0.631080 1_2:+56127398 None:intergenic
GGTGGAAGTACCACCAAACA+CGG 0.631934 1_2:+56129424 None:intergenic
TGTGCCTTGACCACCAAACA+GGG 0.632883 1_2:+56126805 None:intergenic
CAACTTTGCAGCCTGTCCAG+AGG 0.635625 1_2:+56127289 None:intergenic
CGAACCAAATGCTGGTGTTG+AGG 0.636106 1_2:+56128006 None:intergenic
AGACCATTCTCTGCAAAAGG+CGG 0.640474 1_2:-56123437 Msa0078530:CDS
ACTGGGCAACAGTACACTGA+AGG 0.642908 1_2:-56123028 Msa0078530:CDS
AGAAACCTCCTGTTGGACAA+GGG 0.643332 1_2:-56123105 Msa0078530:CDS
TTGGAAGAAAACGGAGACGA+TGG 0.644511 1_2:+56129380 None:intergenic
GCTGCCATTGTGTATGAACA+TGG 0.647605 1_2:-56123376 Msa0078530:CDS
AAAGAAGGTGTAGATTGGAA+AGG 0.648011 1_2:+56125836 None:intergenic
ACCAGACTGTGCATTTGTAG+GGG 0.649924 1_2:+56126721 None:intergenic
AGATACTCCAACCACACCAA+AGG 0.655834 1_2:-56124845 Msa0078530:CDS
AGGGCTGAATAAGCCTGCTG+AGG 0.658660 1_2:-56123086 Msa0078530:CDS
GCTGGTTGCACCAAACGCAG+GGG 0.659543 1_2:+56127928 None:intergenic
AGCTCCATGTTCATACACAA+TGG 0.660594 1_2:+56123372 None:intergenic
GACGGAGGAGCAGAGGCATG+TGG 0.661616 1_2:+56127088 None:intergenic
GATATGTACAAGACACTCAG+TGG 0.662232 1_2:-56123415 Msa0078530:CDS
TGACCCAACTGGCTGTGACG+AGG 0.664975 1_2:+56126835 None:intergenic
CTTCACCAGTTAAAAGACAA+CGG 0.666510 1_2:+56122614 None:intergenic
TGGGTGTGACAGATGTACGA+GGG 0.667447 1_2:-56123192 Msa0078530:CDS
ACTCGACCAATTGACATCTG+AGG 0.669172 1_2:+56125310 None:intergenic
GATGACGAATTACCAAAAGC+CGG 0.670748 1_2:+56129290 None:intergenic
GTGGCGAGAAGTCAATAAGG+AGG 0.671855 1_2:+56125032 None:intergenic
GCTGCTTGTCCAAAACCTGT+CGG 0.672305 1_2:+56126581 None:intergenic
TGTGCTACTAGAGAAAGGAT+TGG 0.673376 1_2:+56127003 None:intergenic
TGAAGTTGAACCAAATGCAG+GGG 0.674521 1_2:+56127808 None:intergenic
CCTCCCATCTTAGCTCCTCG+TGG 0.675080 1_2:+56127228 None:intergenic
ATATGTACAAGACACTCAGT+GGG 0.677051 1_2:-56123414 Msa0078530:CDS
AGACTGACTAAAAGCCGGAG+TGG 0.677239 1_2:+56127601 None:intergenic
CCACGAGGAGCTAAGATGGG+AGG 0.677956 1_2:-56127228 Msa0078530:CDS
AAGGGCATCAGCCTTTGGTG+TGG 0.679934 1_2:+56124834 None:intergenic
TCAGGATTTGGAGGTCAACG+GGG 0.688130 1_2:-56127362 Msa0078530:CDS
TTGGGTGTGACAGATGTACG+AGG 0.689369 1_2:-56123193 Msa0078530:CDS
AGATTGGAAAGGTGTTGACA+TGG 0.692451 1_2:+56125847 None:intergenic
TGGAAAGGTGTTGACATGGA+CGG 0.696227 1_2:+56125851 None:intergenic
TGAACATGGAGCTGATGTTG+AGG 0.697244 1_2:-56123362 Msa0078530:CDS
GGAACCAAAACTAAAACCTG+GGG 0.698294 1_2:+56127625 None:intergenic
GCCGCCAATTCATGAATACG+AGG 0.700929 1_2:+56123298 None:intergenic
AGTTGCGCCAAATACACCGG+TGG 0.703641 1_2:+56129406 None:intergenic
CCCACCACCACCAAACACAG+TGG 0.732038 1_2:+56127739 None:intergenic
CCTGGCATACCAGTTGACGG+AGG 0.738091 1_2:+56127073 None:intergenic
TGTGCAACCATAGAACTCTG+GGG 0.762151 1_2:+56125376 None:intergenic
TTACAAAGATAAAAGCCACG+AGG 0.809043 1_2:-56127243 Msa0078530:CDS
AGTACCATTCTCATTCACGG+AGG 0.817913 1_2:+56124711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATATAGATTTTACAGTT+TGG - chr1_2:56130210-56130229 Msa0078530:five_prime_UTR 15.0%
!!! TTATATAGATTTTACAGTTT+GGG - chr1_2:56130211-56130230 Msa0078530:five_prime_UTR 15.0%
!! TTGATCAGTCATTTAAAAAT+TGG + chr1_2:56129257-56129276 None:intergenic 20.0%
!!! TAACTTCTGTAAATGATTTT+AGG - chr1_2:56129328-56129347 Msa0078530:CDS 20.0%
!!! AACTTCTGTAAATGATTTTA+GGG - chr1_2:56129329-56129348 Msa0078530:CDS 20.0%
!!! AAATGTTTTGACAAAAAAAC+TGG - chr1_2:56129823-56129842 Msa0078530:intron 20.0%
!!! AATGTTTTGACAAAAAAACT+GGG - chr1_2:56129824-56129843 Msa0078530:intron 20.0%
!!! TAAATTGTTTCTCTATTTTG+TGG - chr1_2:56130135-56130154 Msa0078530:intron 20.0%
! AATGGAAATATTATCTGCTA+TGG - chr1_2:56129226-56129245 Msa0078530:intron 25.0%
!!! GAATAAAAGCACCAAAATAA+GGG + chr1_2:56129300-56129319 None:intergenic 25.0%
!!! AGAATAAAAGCACCAAAATA+AGG + chr1_2:56129301-56129320 None:intergenic 25.0%
!! TGTCAAAACATTTTATGTTG+AGG + chr1_2:56129816-56129835 None:intergenic 25.0%
!! GACAATACATTTCATAGTTT+TGG - chr1_2:56130068-56130087 Msa0078530:intron 25.0%
! AAATATATCCCAAAGCAAAA+AGG + chr1_2:56130191-56130210 None:intergenic 25.0%
ACGATTGTTAAACTGAACAA+TGG + chr1_2:56129716-56129735 None:intergenic 30.0%
TCTTTATATTTCCCGATTCT+TGG + chr1_2:56129868-56129887 None:intergenic 30.0%
GTATCATATGATCCCAATAA+AGG - chr1_2:56129928-56129947 Msa0078530:five_prime_UTR 30.0%
AACTTCCATTCTCCTTTATT+GGG + chr1_2:56129943-56129962 None:intergenic 30.0%
GATACAAGCTGATGTATAAA+GGG - chr1_2:56130011-56130030 Msa0078530:intron 30.0%
ACATGTCCCATATAACAATA+TGG - chr1_2:56130037-56130056 Msa0078530:intron 30.0%
CATGTCCCATATAACAATAT+GGG - chr1_2:56130038-56130057 Msa0078530:intron 30.0%
AAAGGACATATCAACAAGAT+TGG + chr1_2:56130173-56130192 None:intergenic 30.0%
! TTGATATGTCCTTTTTGCTT+TGG - chr1_2:56130179-56130198 Msa0078530:five_prime_UTR 30.0%
! TGATATGTCCTTTTTGCTTT+GGG - chr1_2:56130180-56130199 Msa0078530:five_prime_UTR 30.0%
! TATTTCCGTTGTCTTTTAAC+TGG - chr1_2:56130250-56130269 Msa0078530:five_prime_UTR 30.0%
ATATGTACAAGACACTCAGT+GGG - chr1_2:56129455-56129474 Msa0078530:CDS 35.0%
! TTATGGCAGCATCAGATTTT+TGG - chr1_2:56129657-56129676 Msa0078530:intron 35.0%
! TATGGCAGCATCAGATTTTT+GGG - chr1_2:56129658-56129677 Msa0078530:intron 35.0%
TGTTCAGTTTAACAATCGTG+AGG - chr1_2:56129717-56129736 Msa0078530:intron 35.0%
TAAAGAGATGCTCAAGAGAA+AGG - chr1_2:56129882-56129901 Msa0078530:intron 35.0%
ATGATCCCAATAAAGGAGAA+TGG - chr1_2:56129935-56129954 Msa0078530:intron 35.0%
GAACTTCCATTCTCCTTTAT+TGG + chr1_2:56129944-56129963 None:intergenic 35.0%
ATAAAGGAGAATGGAAGTTC+AGG - chr1_2:56129944-56129963 Msa0078530:intron 35.0%
TAAAGGAGAATGGAAGTTCA+GGG - chr1_2:56129945-56129964 Msa0078530:intron 35.0%
GGATACAAGCTGATGTATAA+AGG - chr1_2:56130010-56130029 Msa0078530:intron 35.0%
CCTCTCCCATATTGTTATAT+GGG + chr1_2:56130046-56130065 None:intergenic 35.0%
TCCTCTCCCATATTGTTATA+TGG + chr1_2:56130047-56130066 None:intergenic 35.0%
CTTCACCAGTTAAAAGACAA+CGG + chr1_2:56130258-56130277 None:intergenic 35.0%
AGATCTGCGAGAAGAGATTA+GGG + chr1_2:56122694-56122713 None:intergenic 40.0%
TAGATCTGCGAGAAGAGATT+AGG + chr1_2:56122695-56122714 None:intergenic 40.0%
ACTCAATATGTGGCAA+TGG - chr1_2:56129212-56129231 Msa0078530:intron 40.0%
!! AGCTGAAAATGGTGTTGTCA+AGG - chr1_2:56129357-56129376 Msa0078530:CDS 40.0%
ATGGTCTTCGTTATTCCCAT+TGG + chr1_2:56129419-56129438 None:intergenic 40.0%
GATATGTACAAGACACTCAG+TGG - chr1_2:56129454-56129473 Msa0078530:CDS 40.0%
AGCTCCATGTTCATACACAA+TGG + chr1_2:56129500-56129519 None:intergenic 40.0%
ACTGCCTCGTATTCATGAAT+TGG - chr1_2:56129567-56129586 Msa0078530:intron 40.0%
TATTCATGAATTGGCGGCTA+AGG - chr1_2:56129576-56129595 Msa0078530:intron 40.0%
TCGTGAGGTGATTGTATACA+TGG - chr1_2:56129732-56129751 Msa0078530:intron 40.0%
GATTCAAAGAAACCTCCTGT+TGG - chr1_2:56129757-56129776 Msa0078530:intron 40.0%
TACACTGAAGGACCAAGAAT+CGG - chr1_2:56129853-56129872 Msa0078530:intron 40.0%
ATGATACAAACTCAGCACCT+TGG + chr1_2:56129915-56129934 None:intergenic 40.0%
CCCATATAACAATATGGGAG+AGG - chr1_2:56130043-56130062 Msa0078530:intron 40.0%
! GAGACAGAAATTTTCATCCC+TGG + chr1_2:56130288-56130307 None:intergenic 40.0%
CCAAAATAAGGGACTGATGG+AGG + chr1_2:56129289-56129308 None:intergenic 45.0%
!! CCTCCATCAGTCCCTTATTT+TGG - chr1_2:56129286-56129305 Msa0078530:CDS 45.0%
GCACCAAAATAAGGGACTGA+TGG + chr1_2:56129292-56129311 None:intergenic 45.0%
TTAGGGACTGCAGCTGAAAA+TGG - chr1_2:56129346-56129365 Msa0078530:CDS 45.0%
GTACGAAGCATGCATCCAAT+GGG - chr1_2:56129401-56129420 Msa0078530:CDS 45.0%
CGAAGACCATTCTCTGCAAA+AGG - chr1_2:56129429-56129448 Msa0078530:CDS 45.0%
AGACCATTCTCTGCAAAAGG+CGG - chr1_2:56129432-56129451 Msa0078530:CDS 45.0%
! TATCCGCCTTTTGCAGAGAA+TGG + chr1_2:56129438-56129457 None:intergenic 45.0%
GCTGCCATTGTGTATGAACA+TGG - chr1_2:56129493-56129512 Msa0078530:CDS 45.0%
TGAACATGGAGCTGATGTTG+AGG - chr1_2:56129507-56129526 Msa0078530:CDS 45.0%
GCCTCGTATTCATGAATTGG+CGG - chr1_2:56129570-56129589 Msa0078530:intron 45.0%
TTGACATGACTGCAGAATCC+TGG + chr1_2:56129613-56129632 None:intergenic 45.0%
AGGATTCTGCAGTCATGTCA+AGG - chr1_2:56129612-56129631 Msa0078530:intron 45.0%
CATGTCAAGGACTTTGTCGT+TGG - chr1_2:56129625-56129644 Msa0078530:intron 45.0%
TCAAGGACTTTGTCGTTGGA+AGG - chr1_2:56129629-56129648 Msa0078530:intron 45.0%
AAGAAACCTCCTGTTGGACA+AGG - chr1_2:56129763-56129782 Msa0078530:intron 45.0%
AGAAACCTCCTGTTGGACAA+GGG - chr1_2:56129764-56129783 Msa0078530:intron 45.0%
TTATTCAGCCCTTGTCCAAC+AGG + chr1_2:56129775-56129794 None:intergenic 45.0%
ACACTGAAGGACCAAGAATC+GGG - chr1_2:56129854-56129873 Msa0078530:intron 45.0%
AGCTGGTAGATGAAGACAGT+TGG - chr1_2:56129989-56130008 Msa0078530:intron 45.0%
! TCTATTTTGTGGACAGAGCC+AGG - chr1_2:56130146-56130165 Msa0078530:intron 45.0%
! CTATTTTGTGGACAGAGCCA+GGG - chr1_2:56130147-56130166 Msa0078530:intron 45.0%
! CATATCAACAAGATTGGCCC+TGG + chr1_2:56130167-56130186 None:intergenic 45.0%
AACTGGTGAAGTTTAGTGCC+AGG - chr1_2:56130267-56130286 Msa0078530:five_prime_UTR 45.0%
ACTGGTGAAGTTTAGTGCCA+GGG - chr1_2:56130268-56130287 Msa0078530:five_prime_UTR 45.0%
GATGCATGCTTCGTACGTAC+AGG + chr1_2:56129397-56129416 None:intergenic 50.0%
CGTACGAAGCATGCATCCAA+TGG - chr1_2:56129400-56129419 Msa0078530:CDS 50.0%
CACTAATGCCAAAGCTGCGA+CGG - chr1_2:56129530-56129549 Msa0078530:CDS 50.0%
TAATCAGACCGTCGCAGCTT+TGG + chr1_2:56129541-56129560 None:intergenic 50.0%
GCCGCCAATTCATGAATACG+AGG + chr1_2:56129574-56129593 None:intergenic 50.0%
GCTAAGGAAAGAGCTGAACC+AGG - chr1_2:56129592-56129611 Msa0078530:intron 50.0%
GACTTTGTCGTTGGAAGGCA+AGG - chr1_2:56129634-56129653 Msa0078530:intron 50.0%
GTCGTTGGAAGGCAAGGTTA+TGG - chr1_2:56129640-56129659 Msa0078530:intron 50.0%
TTGGGTGTGACAGATGTACG+AGG - chr1_2:56129676-56129695 Msa0078530:intron 50.0%
TGGGTGTGACAGATGTACGA+GGG - chr1_2:56129677-56129696 Msa0078530:intron 50.0%
!! TTGAGGAGTGTTACCTCAGC+AGG + chr1_2:56129799-56129818 None:intergenic 50.0%
ACTGGGCAACAGTACACTGA+AGG - chr1_2:56129841-56129860 Msa0078530:intron 50.0%
GATGCTCAAGAGAAAGGCTG+AGG - chr1_2:56129888-56129907 Msa0078530:intron 50.0%
AAGAGAAAGGCTGAGGACCA+AGG - chr1_2:56129895-56129914 Msa0078530:exon 50.0%
CCAGCTTGTAAACGCTGAAG+TGG + chr1_2:56129975-56129994 None:intergenic 50.0%
CCACTTCAGCGTTTACAAGC+TGG - chr1_2:56129972-56129991 Msa0078530:intron 50.0%
TTCAGCCCTTGTCCAACAGG+AGG + chr1_2:56129772-56129791 None:intergenic 55.0%
AGGGCTGAATAAGCCTGCTG+AGG - chr1_2:56129783-56129802 Msa0078530:intron 55.0%
ACACTCAGTGGGCACAGAGC+TGG - chr1_2:56129466-56129485 Msa0078530:CDS 60.0%
CAGTGGGCACAGAGCTGGTG+AGG - chr1_2:56129471-56129490 Msa0078530:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 56122556 56130335 56122556 ID=Msa0078530;Name=Msa0078530
chr1_2 mRNA 56122556 56130335 56122556 ID=Msa0078530-mRNA-1;Parent=Msa0078530;Name=Msa0078530-mRNA-1;_AED=0.32;_eAED=0.32;_QI=219|0.90|0.91|1|0.90|0.91|12|306|999
chr1_2 exon 56122556 56123541 56122556 ID=Msa0078530-mRNA-1:exon:17553;Parent=Msa0078530-mRNA-1
chr1_2 exon 56124716 56124890 56124716 ID=Msa0078530-mRNA-1:exon:17552;Parent=Msa0078530-mRNA-1
chr1_2 exon 56124975 56125085 56124975 ID=Msa0078530-mRNA-1:exon:17551;Parent=Msa0078530-mRNA-1
chr1_2 exon 56125267 56125405 56125267 ID=Msa0078530-mRNA-1:exon:17550;Parent=Msa0078530-mRNA-1
chr1_2 exon 56125613 56125679 56125613 ID=Msa0078530-mRNA-1:exon:17549;Parent=Msa0078530-mRNA-1
chr1_2 exon 56125786 56125873 56125786 ID=Msa0078530-mRNA-1:exon:17548;Parent=Msa0078530-mRNA-1
chr1_2 exon 56126580 56127115 56126580 ID=Msa0078530-mRNA-1:exon:17547;Parent=Msa0078530-mRNA-1
chr1_2 exon 56127207 56127431 56127207 ID=Msa0078530-mRNA-1:exon:17546;Parent=Msa0078530-mRNA-1
chr1_2 exon 56127528 56128184 56127528 ID=Msa0078530-mRNA-1:exon:17545;Parent=Msa0078530-mRNA-1
chr1_2 exon 56129279 56129581 56129279 ID=Msa0078530-mRNA-1:exon:17544;Parent=Msa0078530-mRNA-1
chr1_2 exon 56129892 56129953 56129892 ID=Msa0078530-mRNA-1:exon:17543;Parent=Msa0078530-mRNA-1
chr1_2 exon 56130160 56130335 56130160 ID=Msa0078530-mRNA-1:exon:17542;Parent=Msa0078530-mRNA-1
chr1_2 five_prime_UTR 56130160 56130335 56130160 ID=Msa0078530-mRNA-1:five_prime_utr;Parent=Msa0078530-mRNA-1
chr1_2 five_prime_UTR 56129911 56129953 56129911 ID=Msa0078530-mRNA-1:five_prime_utr;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56129892 56129910 56129892 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56129279 56129581 56129279 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56127528 56128184 56127528 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56127207 56127431 56127207 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56126580 56127115 56126580 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56125786 56125873 56125786 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56125613 56125679 56125613 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56125267 56125405 56125267 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56124975 56125085 56124975 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56124716 56124890 56124716 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 CDS 56122862 56123541 56122862 ID=Msa0078530-mRNA-1:cds;Parent=Msa0078530-mRNA-1
chr1_2 three_prime_UTR 56122556 56122861 56122556 ID=Msa0078530-mRNA-1:three_prime_utr;Parent=Msa0078530-mRNA-1
Gene Sequence

>Msa0078530

ATGTTTGGTTCAACAAATCCTTTCGGGCAGTCATCGAGCCCGTTCGGCTCGTCTCAATCCGTGTTCGGGCAGCAGAACAACACAAATAACAATCCTTTTGCACCTAAGCCATTTGGGAGTCCAACTCCATTTGGATCGCAGCAAACGGGTAATTCCGTGTTTGGTGGTACTTCCACCGGTGTATTTGGCGCAACTCAGCCATCGTCTCCGTTTTCTTCCAATACTTTCGGCGCTTCATCATCTCCAGCTTTTGGTACTTCAATGCCGGCATTTGGGGCATCTTCAACTCCGGCTTTTGGTAATTCGTCATCGTCATTTGGTGGATCATCAGTGTTTGGTCAGAAGCCTTCTTTTGGAGGTTTTGGCTCTACTCCTACTCAAACAAGTCCATTTGGAAGTGCCACCCAACCATCACAACCAGCATTTGGAAGTAACACCCAACAGTCGCAATCAGCATTTGGAAGCAGCATATTTGGTTCCTCAACACCAGCATTTGGTTCGACAGGCACTCCTGCATTTGGTGCTACGAGCACTCCTGCATTTGGTGCTACGAGCACCCCTGCGTTTGGTGCAACCAGCACCCCTGCTTTTGGTGCACCCAGCACCCCTGCTTTTGGTGCACCCAGCGCCCCAGCTTTCGGTGCAACCAGCACCCCAGCTTTTGGGGCAACCAGCACCCCTGCATTTGGTTCAACTTCAACCTCAGCTTTTGGTAACACAGGGGGTGCATTTGGCATGTCAAATACCACTGTGTTTGGTGGTGGTGGGGGATTTGGGGCTTCAAGCAGTCCTGTATTTGGTTCATCAAGCACGCCTGCATTTGGGGCCTCCAGCTCTCCTGCTTTTGGTGCTTCTAGTGCCCCAGGTTTTAGTTTTGGTTCCACCACTCCGGCTTTTAGTCAGTCTAGTTCTGCATTTGGCACCAGCAGCCCATTTGGGAGTACGACCTCTAATTTTGGAGGTCAAAGTTCTGCATTTGGATCACAGACTCCCACTCAAGCATTTGGAAGTACTGGTATTGGGCAGTCAGGATTTGGAGGTCAACGGGGTGGAAGTAGAATAGCTAGTTACTCAGCTACAGTGGAAGCAGATAGTGGCACCTCTGGACAGGCTGCAAAGTTGGAGTCAATATCAGCCATGCCTGTTTACAAAGATAAAAGCCACGAGGAGCTAAGATGGGAGGACTATCAATTGGGAGATAAAGGTGGACCACATGCCTCTGCTCCTCCGTCAACTGGTATGCCAGGCTTTAATTCGTCTACGACACAGACAAATGTTTTTGGTCAATCACCAGCCAATCCTTTCTCTAGTAGCACAACACCTAATTCTAACCCATTTGCACAAAAGACATTTGGAACTTCAGCTCCTGCCTTCAGTTCACCAGCATTTCCTTCTTCATCATCATCAGCAGCAGCACCCTCTATTTTTGGAACACCCGCAACTGCTCCGTTTGGGGCCAACTCCTCGTCACAGCCAGTTGGGTCATCTACATCCCTGTTTGGTGGTCAAGGCACATCTTCCCAATTTAGTACAAACATTTTTGGCAACACCCAGCCAAGCCTATTTAACACTTCTGCCCCTACAAATGCACAGTCTGGTTTTGGTCAGAATACCTCCCAATTTGGGCAAACTGGTGCTTTTAATCAACCAAGCATGTTCAATCAACCTTCATCTGGAAGCCTTTTCTCAAGCAGCCCATCACTCACATCTAATGCTCCGACAGGTTTTGGACAAGCAGCGCCGTCCATGTCAACACCTTTCCAATCTACACCTTCTTTCCAATCGGCTGGTACCTTTTCCTTTAGCAACTTTGGCCAGACACAACCTGCTGGTGCAAGTAGCTTTGCTGGAGCTCAAACTCAAAGCATGTTTGGTCAGAATAATGTTGGACTTCAGGCTATTCCCCAGAGTTCTATGGTTGCACAAGCAGCACCCATTACAAACCCATTTGGAACACTTCCTGCTTTGCCTCAGATGTCAATTGGTCGAGTTGGAACCACTCCTTCCGTTCAATATGGTATCTCTAGCATGCCTGCCCAAGACAAACCTGCTACTGTAAGAATATCCTCCTTATTGACTTCTCGCCACCTCTCACAAAGAAGAGTCAAGTTTCCTATAAGGAAATATAAGAATGAGGGATCAAAGGTTCCATTCTTTAGTGATGATGAAGATACTCCAACCACACCAAAGGCTGATGCCCTTTTCATTCCTAGGGAAAATCCAAGGGCGTTGATCATTTGTCCCATGGAGCAGTGGCCTGGAAGGACTTCTGAGAAAGCATCCTCTTTTAAGGATAGATACACCTCCGTGAATGAGAATGGGACTGCAGCTGAAAATGGTGTTGTCAAGGAGCAAGTTCAGCAGCCTGTACGTACGAAGCATGCATCCAATGGGAATAACGAAGACCATTCTCTGCAAAAGGCGGATATGTACAAGACACTCAGTGGGCACAGAGCTGGTGAGGCTGCCATTGTGTATGAACATGGAGCTGATGTTGAGGCACTAATGCCAAAGCTGCGACGGTCTGATTACTACACACTGCCTCGTATTCATGAATTGGCGGCTAAGGAAAGAGCTGAACCAGGATTCTGCAGTCATGTCAAGGACTTTGTCGTTGGAAGGCAAGGTTATGGCAGCATCAGATTTTTGGGTGTGACAGATGTACGAGGGATTGATCTCGAGTCCATTGTTCAGTTTAACAATCGTGAGGTGATTGTATACATGGATGATTCAAAGAAACCTCCTGTTGGACAAGGGCTGAATAAGCCTGCTGAGGTAACACTCCTCAACATAAAATGTTTTGACAAAAAAACTGGGCAACAGTACACTGAAGGACCAAGAATCGGGAAATATAAAGAGATGCTCAAGAGAAAGGCTGAGGACCAAGGTGCTGAGTTTGTATCATATGATCCCAATAAAGGAGAATGGAAGTTCAGGGTCAACCACTTCAGCGTTTACAAGCTGGTAGATGAAGACAGTTGGATACAAGCTGATGTATAA
Protein sequence

>Msa0078530

MFGSTNPFGQSSSPFGSSQSVFGQQNNTNNNPFAPKPFGSPTPFGSQQTGNSVFGGTSTGVFGATQPSSPFSSNTFGASSSPAFGTSMPAFGASSTPAFGNSSSSFGGSSVFGQKPSFGGFGSTPTQTSPFGSATQPSQPAFGSNTQQSQSAFGSSIFGSSTPAFGSTGTPAFGATSTPAFGATSTPAFGATSTPAFGAPSTPAFGAPSAPAFGATSTPAFGATSTPAFGSTSTSAFGNTGGAFGMSNTTVFGGGGGFGASSSPVFGSSSTPAFGASSSPAFGASSAPGFSFGSTTPAFSQSSSAFGTSSPFGSTTSNFGGQSSAFGSQTPTQAFGSTGIGQSGFGGQRGGSRIASYSATVEADSGTSGQAAKLESISAMPVYKDKSHEELRWEDYQLGDKGGPHASAPPSTGMPGFNSSTTQTNVFGQSPANPFSSSTTPNSNPFAQKTFGTSAPAFSSPAFPSSSSSAAAPSIFGTPATAPFGANSSSQPVGSSTSLFGGQGTSSQFSTNIFGNTQPSLFNTSAPTNAQSGFGQNTSQFGQTGAFNQPSMFNQPSSGSLFSSSPSLTSNAPTGFGQAAPSMSTPFQSTPSFQSAGTFSFSNFGQTQPAGASSFAGAQTQSMFGQNNVGLQAIPQSSMVAQAAPITNPFGTLPALPQMSIGRVGTTPSVQYGISSMPAQDKPATVRISSLLTSRHLSQRRVKFPIRKYKNEGSKVPFFSDDEDTPTTPKADALFIPRENPRALIICPMEQWPGRTSEKASSFKDRYTSVNENGTAAENGVVKEQVQQPVRTKHASNGNNEDHSLQKADMYKTLSGHRAGEAAIVYEHGADVEALMPKLRRSDYYTLPRIHELAAKERAEPGFCSHVKDFVVGRQGYGSIRFLGVTDVRGIDLESIVQFNNREVIVYMDDSKKPPVGQGLNKPAEVTLLNIKCFDKKTGQQYTEGPRIGKYKEMLKRKAEDQGAEFVSYDPNKGEWKFRVNHFSVYKLVDEDSWIQADV*