AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0102020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTCTGATGTGGATAAA+AGG 0.215914 1_3:+8726985 Msa0102020:five_prime_UTR
TAGGAATACTTATAACTAAA+TGG 0.255585 1_3:-8727042 None:intergenic
AAATGGTGGATTCTTGAAAT+TGG 0.255850 1_3:-8727025 None:intergenic
AAAACTGTTATTTGCAAAAC+AGG 0.340762 1_3:-8727364 None:intergenic
CACCGATACAAGAGCCTTGA+TGG 0.355752 1_3:+8728001 Msa0102020:CDS
ACCACGTGGTGGTTGACTTT+GGG 0.382216 1_3:+8726839 Msa0102020:five_prime_UTR
CATTCTCTGGATTGGTGAAT+CGG 0.407829 1_3:-8726921 None:intergenic
TCCAGAGAATGATGTTAATA+CGG 0.428214 1_3:+8726933 Msa0102020:five_prime_UTR
CTAGTTCTTGGCAGTGTCAA+AGG 0.429450 1_3:+8727279 Msa0102020:intron
ATATGTAAATGAAACGATAT+CGG 0.432583 1_3:-8726751 None:intergenic
AGATATCCAGAAAATTATAA+TGG 0.438771 1_3:-8727390 None:intergenic
ATTAACATCATTCTCTGGAT+TGG 0.440029 1_3:-8726929 None:intergenic
AACATCCTGTGTGAACATAA+TGG 0.446340 1_3:-8727080 None:intergenic
AACCACGTGGTGGTTGACTT+TGG 0.454759 1_3:+8726838 Msa0102020:five_prime_UTR
CCCGTATTAACATCATTCTC+TGG 0.461431 1_3:-8726934 None:intergenic
CTGCAGATGATGATGACATC+AGG 0.461918 1_3:+8727967 Msa0102020:intron
TAGTTCTTGGCAGTGTCAAA+GGG 0.472596 1_3:+8727280 Msa0102020:intron
TGATTCTGATGTGGATAAAA+GGG 0.477448 1_3:+8726986 Msa0102020:five_prime_UTR
ATGGCCATGAGTATCTTTGT+AGG 0.493172 1_3:-8727061 None:intergenic
TGATGTGGATAAAAGGGAAT+GGG 0.494248 1_3:+8726992 Msa0102020:exon
CCAGAGAATGATGTTAATAC+GGG 0.496258 1_3:+8726934 Msa0102020:five_prime_UTR
CTGATGTGGATAAAAGGGAA+TGG 0.503803 1_3:+8726991 Msa0102020:exon
TGGAGTTCAAAGCTGGAGTA+AGG 0.508936 1_3:+8728021 Msa0102020:CDS
CTCCATCAAGGCTCTTGTAT+CGG 0.512255 1_3:-8728003 Msa0102030:intron
GCCTTGATGGAGTTCAAAGC+TGG 0.517462 1_3:+8728014 Msa0102020:CDS
CAAAGGGGAAGGCGTGGAGA+TGG 0.519317 1_3:+8727296 Msa0102020:CDS
CTTAAGCTTTGATTCTGATG+TGG 0.524480 1_3:+8726977 Msa0102020:five_prime_UTR
GGGTTCGAATTCCATAAAAG+TGG 0.534911 1_3:+8726859 Msa0102020:five_prime_UTR
TCCAGCTTTGAACTCCATCA+AGG 0.536851 1_3:-8728015 Msa0102030:intron
ATCTTGGGAAGCATGCCTAC+TGG 0.545900 1_3:+8727409 Msa0102020:CDS
GAAAGAGAAAAGTAGCATGA+TGG 0.558487 1_3:+8726894 Msa0102020:five_prime_UTR
CTTGGCAGTGTCAAAGGGGA+AGG 0.578073 1_3:+8727285 Msa0102020:CDS
CAGTGTCAAAGGGGAAGGCG+TGG 0.616979 1_3:+8727290 Msa0102020:CDS
GGAGATGGATCTTTCTGTCG+TGG 0.620374 1_3:+8727311 Msa0102020:CDS
TATTCCTACAAAGATACTCA+TGG 0.630332 1_3:+8727057 Msa0102020:CDS
GAATACTTATAACTAAATGG+TGG 0.631665 1_3:-8727039 None:intergenic
ATCTCGAGGCTGAATATGTG+TGG 0.634545 1_3:+8726728 Msa0102020:five_prime_UTR
AGTTCTTGGCAGTGTCAAAG+GGG 0.674918 1_3:+8727281 Msa0102020:intron
CAGAGAATGATGTTAATACG+GGG 0.686813 1_3:+8726935 Msa0102020:five_prime_UTR
CATGGCCATTATGTTCACAC+AGG 0.696824 1_3:+8727075 Msa0102020:CDS
AGAAAATGAGTGGAACCACG+TGG 0.729935 1_3:+8726825 Msa0102020:five_prime_UTR
ACCCAAAGTCAACCACCACG+TGG 0.730047 1_3:-8726840 None:intergenic
AAATGAGTGGAACCACGTGG+TGG 0.778870 1_3:+8726828 Msa0102020:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAGAAAAAGTTAAAATT+CGG - chr1_3:8727261-8727280 None:intergenic 15.0%
!! AGAAAGAAAAAGTTAAAATT+CGG - chr1_3:8727261-8727280 None:intergenic 15.0%
!! ATATGTAAATGAAACGATAT+CGG - chr1_3:8726754-8726773 None:intergenic 20.0%
!! TAGGAATACTTATAACTAAA+TGG - chr1_3:8727045-8727064 None:intergenic 20.0%
!! AGATATCCAGAAAATTATAA+TGG - chr1_3:8727393-8727412 None:intergenic 20.0%
!!! TTATAATTTTCTGGATATCT+TGG + chr1_3:8727393-8727412 Msa0102020:CDS 20.0%
!!! TATAATTTTCTGGATATCTT+GGG + chr1_3:8727394-8727413 Msa0102020:CDS 20.0%
!! ATATGTAAATGAAACGATAT+CGG - chr1_3:8726754-8726773 None:intergenic 20.0%
!! TAGGAATACTTATAACTAAA+TGG - chr1_3:8727045-8727064 None:intergenic 20.0%
!! AGATATCCAGAAAATTATAA+TGG - chr1_3:8727393-8727412 None:intergenic 20.0%
!!! TTATAATTTTCTGGATATCT+TGG + chr1_3:8727393-8727412 Msa0102020:CDS 20.0%
!!! TATAATTTTCTGGATATCTT+GGG + chr1_3:8727394-8727413 Msa0102020:CDS 20.0%
! GAATACTTATAACTAAATGG+TGG - chr1_3:8727042-8727061 None:intergenic 25.0%
!!! AACTTTTTCTTTCTAGTTCT+TGG + chr1_3:8727267-8727286 Msa0102020:intron 25.0%
!! AAAACTGTTATTTGCAAAAC+AGG - chr1_3:8727367-8727386 None:intergenic 25.0%
!! GTTTTGCAAATAACAGTTTT+TGG + chr1_3:8727367-8727386 Msa0102020:CDS 25.0%
!! TTTTGGCCATTATAATTTTC+TGG + chr1_3:8727384-8727403 Msa0102020:CDS 25.0%
! TGAAATATGTGTTTCTTTGT+TGG + chr1_3:8727552-8727571 Msa0102020:intron 25.0%
! GAATACTTATAACTAAATGG+TGG - chr1_3:8727042-8727061 None:intergenic 25.0%
!!! AACTTTTTCTTTCTAGTTCT+TGG + chr1_3:8727267-8727286 Msa0102020:intron 25.0%
!! AAAACTGTTATTTGCAAAAC+AGG - chr1_3:8727367-8727386 None:intergenic 25.0%
!! GTTTTGCAAATAACAGTTTT+TGG + chr1_3:8727367-8727386 Msa0102020:CDS 25.0%
!! TTTTGGCCATTATAATTTTC+TGG + chr1_3:8727384-8727403 Msa0102020:CDS 25.0%
! TGAAATATGTGTTTCTTTGT+TGG + chr1_3:8727552-8727571 Msa0102020:intron 25.0%
! ATGGCAAAACATTTTTCTAC+AGG + chr1_3:8726704-8726723 Msa0102020:five_prime_UTR 30.0%
! TGGCAAAACATTTTTCTACA+GGG + chr1_3:8726705-8726724 Msa0102020:five_prime_UTR 30.0%
! TGAAAAATTCAATGATGACG+TGG + chr1_3:8726789-8726808 Msa0102020:five_prime_UTR 30.0%
! TACTTTTCTCTTTCAACACA+TGG - chr1_3:8726888-8726907 None:intergenic 30.0%
ATTAACATCATTCTCTGGAT+TGG - chr1_3:8726932-8726951 None:intergenic 30.0%
TCCAGAGAATGATGTTAATA+CGG + chr1_3:8726933-8726952 Msa0102020:five_prime_UTR 30.0%
TTGATTCTGATGTGGATAAA+AGG + chr1_3:8726985-8727004 Msa0102020:five_prime_UTR 30.0%
TGATTCTGATGTGGATAAAA+GGG + chr1_3:8726986-8727005 Msa0102020:five_prime_UTR 30.0%
!! AAATGGTGGATTCTTGAAAT+TGG - chr1_3:8727028-8727047 None:intergenic 30.0%
TATTCCTACAAAGATACTCA+TGG + chr1_3:8727057-8727076 Msa0102020:CDS 30.0%
! CATAGCAGAAATTCTTTTTG+AGG + chr1_3:8727623-8727642 Msa0102020:intron 30.0%
TCTGAAGAAGTTAAAATCTC+TGG - chr1_3:8727774-8727793 Msa0102030:CDS 30.0%
! TTTAACTTCTTCAGATTCAG+TGG + chr1_3:8727779-8727798 Msa0102020:intron 30.0%
ATCATCATCTGCAGAAAATT+TGG - chr1_3:8727961-8727980 Msa0102030:intron 30.0%
! ATGGCAAAACATTTTTCTAC+AGG + chr1_3:8726704-8726723 Msa0102020:five_prime_UTR 30.0%
! TGGCAAAACATTTTTCTACA+GGG + chr1_3:8726705-8726724 Msa0102020:five_prime_UTR 30.0%
! TGAAAAATTCAATGATGACG+TGG + chr1_3:8726789-8726808 Msa0102020:five_prime_UTR 30.0%
! TACTTTTCTCTTTCAACACA+TGG - chr1_3:8726888-8726907 None:intergenic 30.0%
ATTAACATCATTCTCTGGAT+TGG - chr1_3:8726932-8726951 None:intergenic 30.0%
TCCAGAGAATGATGTTAATA+CGG + chr1_3:8726933-8726952 Msa0102020:five_prime_UTR 30.0%
TTGATTCTGATGTGGATAAA+AGG + chr1_3:8726985-8727004 Msa0102020:five_prime_UTR 30.0%
TGATTCTGATGTGGATAAAA+GGG + chr1_3:8726986-8727005 Msa0102020:five_prime_UTR 30.0%
!! AAATGGTGGATTCTTGAAAT+TGG - chr1_3:8727028-8727047 None:intergenic 30.0%
TATTCCTACAAAGATACTCA+TGG + chr1_3:8727057-8727076 Msa0102020:CDS 30.0%
! CATAGCAGAAATTCTTTTTG+AGG + chr1_3:8727623-8727642 Msa0102020:intron 30.0%
TCTGAAGAAGTTAAAATCTC+TGG - chr1_3:8727774-8727793 Msa0102030:CDS 30.0%
! TTTAACTTCTTCAGATTCAG+TGG + chr1_3:8727779-8727798 Msa0102020:intron 30.0%
ATCATCATCTGCAGAAAATT+TGG - chr1_3:8727961-8727980 Msa0102030:intron 30.0%
!! CAGCACTTTTAGAAAATGAG+TGG + chr1_3:8726815-8726834 Msa0102020:five_prime_UTR 35.0%
!! ACACATGGATTCCACTTTTA+TGG - chr1_3:8726873-8726892 None:intergenic 35.0%
GAAAGAGAAAAGTAGCATGA+TGG + chr1_3:8726894-8726913 Msa0102020:five_prime_UTR 35.0%
CCAGAGAATGATGTTAATAC+GGG + chr1_3:8726934-8726953 Msa0102020:five_prime_UTR 35.0%
CAGAGAATGATGTTAATACG+GGG + chr1_3:8726935-8726954 Msa0102020:five_prime_UTR 35.0%
! CTTAAGCTTTGATTCTGATG+TGG + chr1_3:8726977-8726996 Msa0102020:five_prime_UTR 35.0%
TGATGTGGATAAAAGGGAAT+GGG + chr1_3:8726992-8727011 Msa0102020:exon 35.0%
AACATCCTGTGTGAACATAA+TGG - chr1_3:8727083-8727102 None:intergenic 35.0%
AGAATTTCTGCTATGTATGC+AGG - chr1_3:8727618-8727637 None:intergenic 35.0%
ACCTTCTTCGAGAAATTAGA+AGG + chr1_3:8727668-8727687 Msa0102020:intron 35.0%
TCCTTCTAATTTCTCGAAGA+AGG - chr1_3:8727672-8727691 None:intergenic 35.0%
TGTGAGGTCTAAATCTTTCA+AGG - chr1_3:8727735-8727754 None:intergenic 35.0%
! AAGTCCTACACTGTTTTCAA+AGG - chr1_3:8727864-8727883 Msa0102030:CDS 35.0%
TTCATGACAGTACACTATTC+AGG - chr1_3:8727918-8727937 Msa0102030:intron 35.0%
!! CAGCACTTTTAGAAAATGAG+TGG + chr1_3:8726815-8726834 Msa0102020:five_prime_UTR 35.0%
!! ACACATGGATTCCACTTTTA+TGG - chr1_3:8726873-8726892 None:intergenic 35.0%
GAAAGAGAAAAGTAGCATGA+TGG + chr1_3:8726894-8726913 Msa0102020:five_prime_UTR 35.0%
CCAGAGAATGATGTTAATAC+GGG + chr1_3:8726934-8726953 Msa0102020:five_prime_UTR 35.0%
CAGAGAATGATGTTAATACG+GGG + chr1_3:8726935-8726954 Msa0102020:five_prime_UTR 35.0%
! CTTAAGCTTTGATTCTGATG+TGG + chr1_3:8726977-8726996 Msa0102020:five_prime_UTR 35.0%
TGATGTGGATAAAAGGGAAT+GGG + chr1_3:8726992-8727011 Msa0102020:exon 35.0%
AACATCCTGTGTGAACATAA+TGG - chr1_3:8727083-8727102 None:intergenic 35.0%
AGAATTTCTGCTATGTATGC+AGG - chr1_3:8727618-8727637 None:intergenic 35.0%
ACCTTCTTCGAGAAATTAGA+AGG + chr1_3:8727668-8727687 Msa0102020:intron 35.0%
TCCTTCTAATTTCTCGAAGA+AGG - chr1_3:8727672-8727691 None:intergenic 35.0%
TGTGAGGTCTAAATCTTTCA+AGG - chr1_3:8727735-8727754 None:intergenic 35.0%
! AAGTCCTACACTGTTTTCAA+AGG - chr1_3:8727864-8727883 Msa0102030:CDS 35.0%
TTCATGACAGTACACTATTC+AGG - chr1_3:8727918-8727937 Msa0102030:intron 35.0%
! ATTTTTCTACAGGGATCTCG+AGG + chr1_3:8726714-8726733 Msa0102020:five_prime_UTR 40.0%
GGGTTCGAATTCCATAAAAG+TGG + chr1_3:8726859-8726878 Msa0102020:five_prime_UTR 40.0%
!! CATTCTCTGGATTGGTGAAT+CGG - chr1_3:8726924-8726943 None:intergenic 40.0%
CCCGTATTAACATCATTCTC+TGG - chr1_3:8726937-8726956 None:intergenic 40.0%
CTGATGTGGATAAAAGGGAA+TGG + chr1_3:8726991-8727010 Msa0102020:exon 40.0%
ATGGCCATGAGTATCTTTGT+AGG - chr1_3:8727064-8727083 None:intergenic 40.0%
TAGTTCTTGGCAGTGTCAAA+GGG + chr1_3:8727280-8727299 Msa0102020:intron 40.0%
ATGTGTTTCTTTGTTGGCCA+AGG + chr1_3:8727558-8727577 Msa0102020:intron 40.0%
TAGAAGGATTCATCACGTGT+AGG + chr1_3:8727684-8727703 Msa0102020:intron 40.0%
CAGATTCAGTGGAATCTGTA+AGG + chr1_3:8727790-8727809 Msa0102020:intron 40.0%
! GCAACCTTTGAAAACAGTGT+AGG + chr1_3:8727857-8727876 Msa0102020:intron 40.0%
CATCTGCAGAAAATTTGGAG+AGG - chr1_3:8727956-8727975 Msa0102030:intron 40.0%
! ATTTTTCTACAGGGATCTCG+AGG + chr1_3:8726714-8726733 Msa0102020:five_prime_UTR 40.0%
GGGTTCGAATTCCATAAAAG+TGG + chr1_3:8726859-8726878 Msa0102020:five_prime_UTR 40.0%
!! CATTCTCTGGATTGGTGAAT+CGG - chr1_3:8726924-8726943 None:intergenic 40.0%
CCCGTATTAACATCATTCTC+TGG - chr1_3:8726937-8726956 None:intergenic 40.0%
CTGATGTGGATAAAAGGGAA+TGG + chr1_3:8726991-8727010 Msa0102020:exon 40.0%
ATGGCCATGAGTATCTTTGT+AGG - chr1_3:8727064-8727083 None:intergenic 40.0%
TAGTTCTTGGCAGTGTCAAA+GGG + chr1_3:8727280-8727299 Msa0102020:intron 40.0%
ATGTGTTTCTTTGTTGGCCA+AGG + chr1_3:8727558-8727577 Msa0102020:intron 40.0%
TAGAAGGATTCATCACGTGT+AGG + chr1_3:8727684-8727703 Msa0102020:intron 40.0%
CAGATTCAGTGGAATCTGTA+AGG + chr1_3:8727790-8727809 Msa0102020:intron 40.0%
! GCAACCTTTGAAAACAGTGT+AGG + chr1_3:8727857-8727876 Msa0102020:intron 40.0%
CATCTGCAGAAAATTTGGAG+AGG - chr1_3:8727956-8727975 Msa0102030:intron 40.0%
ATCTCGAGGCTGAATATGTG+TGG + chr1_3:8726728-8726747 Msa0102020:five_prime_UTR 45.0%
AGAAAATGAGTGGAACCACG+TGG + chr1_3:8726825-8726844 Msa0102020:five_prime_UTR 45.0%
CATGGCCATTATGTTCACAC+AGG + chr1_3:8727075-8727094 Msa0102020:CDS 45.0%
! CTAGTTCTTGGCAGTGTCAA+AGG + chr1_3:8727279-8727298 Msa0102020:intron 45.0%
AGTTCTTGGCAGTGTCAAAG+GGG + chr1_3:8727281-8727300 Msa0102020:intron 45.0%
ACAGAAAGAGACCTACCAGT+AGG - chr1_3:8727427-8727446 None:intergenic 45.0%
GTCTCTACTCTACTAGTGCA+CGG + chr1_3:8727491-8727510 Msa0102020:intron 45.0%
CATGTCCAACTCTTTCACCT+TGG - chr1_3:8727578-8727597 None:intergenic 45.0%
!! TGTAGGAGCATTCCCTCAAA+AGG + chr1_3:8727701-8727720 Msa0102020:intron 45.0%
! AAGGATGTGCAGCCTTTTGA+GGG - chr1_3:8727716-8727735 None:intergenic 45.0%
! TGTATGCAGCAAGAAGTGTG+AGG - chr1_3:8727751-8727770 Msa0102030:CDS 45.0%
TGCTCGAAGTCTAAGTGAAG+AGG + chr1_3:8727830-8727849 Msa0102020:intron 45.0%
! CTGCAGATGATGATGACATC+AGG + chr1_3:8727967-8727986 Msa0102020:intron 45.0%
CTCCATCAAGGCTCTTGTAT+CGG - chr1_3:8728006-8728025 Msa0102030:intron 45.0%
!! TCCAGCTTTGAACTCCATCA+AGG - chr1_3:8728018-8728037 Msa0102030:intron 45.0%
TGGAGTTCAAAGCTGGAGTA+AGG + chr1_3:8728021-8728040 Msa0102020:CDS 45.0%
ATCTCGAGGCTGAATATGTG+TGG + chr1_3:8726728-8726747 Msa0102020:five_prime_UTR 45.0%
AGAAAATGAGTGGAACCACG+TGG + chr1_3:8726825-8726844 Msa0102020:five_prime_UTR 45.0%
CATGGCCATTATGTTCACAC+AGG + chr1_3:8727075-8727094 Msa0102020:CDS 45.0%
! CTAGTTCTTGGCAGTGTCAA+AGG + chr1_3:8727279-8727298 Msa0102020:intron 45.0%
AGTTCTTGGCAGTGTCAAAG+GGG + chr1_3:8727281-8727300 Msa0102020:intron 45.0%
ACAGAAAGAGACCTACCAGT+AGG - chr1_3:8727427-8727446 None:intergenic 45.0%
GTCTCTACTCTACTAGTGCA+CGG + chr1_3:8727491-8727510 Msa0102020:intron 45.0%
CATGTCCAACTCTTTCACCT+TGG - chr1_3:8727578-8727597 None:intergenic 45.0%
!! TGTAGGAGCATTCCCTCAAA+AGG + chr1_3:8727701-8727720 Msa0102020:intron 45.0%
! AAGGATGTGCAGCCTTTTGA+GGG - chr1_3:8727716-8727735 None:intergenic 45.0%
! TGTATGCAGCAAGAAGTGTG+AGG - chr1_3:8727751-8727770 Msa0102030:CDS 45.0%
TGCTCGAAGTCTAAGTGAAG+AGG + chr1_3:8727830-8727849 Msa0102020:intron 45.0%
! CTGCAGATGATGATGACATC+AGG + chr1_3:8727967-8727986 Msa0102020:intron 45.0%
CTCCATCAAGGCTCTTGTAT+CGG - chr1_3:8728006-8728025 Msa0102030:intron 45.0%
!! TCCAGCTTTGAACTCCATCA+AGG - chr1_3:8728018-8728037 Msa0102030:intron 45.0%
TGGAGTTCAAAGCTGGAGTA+AGG + chr1_3:8728021-8728040 Msa0102020:CDS 45.0%
AAATGAGTGGAACCACGTGG+TGG + chr1_3:8726828-8726847 Msa0102020:five_prime_UTR 50.0%
! AACCACGTGGTGGTTGACTT+TGG + chr1_3:8726838-8726857 Msa0102020:five_prime_UTR 50.0%
! ACCACGTGGTGGTTGACTTT+GGG + chr1_3:8726839-8726858 Msa0102020:five_prime_UTR 50.0%
GGAGATGGATCTTTCTGTCG+TGG + chr1_3:8727311-8727330 Msa0102020:CDS 50.0%
! ATCTTGGGAAGCATGCCTAC+TGG + chr1_3:8727409-8727428 Msa0102020:CDS 50.0%
GTTGGCCAAGGTGAAAGAGT+TGG + chr1_3:8727570-8727589 Msa0102020:intron 50.0%
! CAAGGATGTGCAGCCTTTTG+AGG - chr1_3:8727717-8727736 None:intergenic 50.0%
CACCGATACAAGAGCCTTGA+TGG + chr1_3:8728001-8728020 Msa0102020:CDS 50.0%
GCCTTGATGGAGTTCAAAGC+TGG + chr1_3:8728014-8728033 Msa0102020:CDS 50.0%
AAATGAGTGGAACCACGTGG+TGG + chr1_3:8726828-8726847 Msa0102020:five_prime_UTR 50.0%
! AACCACGTGGTGGTTGACTT+TGG + chr1_3:8726838-8726857 Msa0102020:five_prime_UTR 50.0%
! ACCACGTGGTGGTTGACTTT+GGG + chr1_3:8726839-8726858 Msa0102020:five_prime_UTR 50.0%
GGAGATGGATCTTTCTGTCG+TGG + chr1_3:8727311-8727330 Msa0102020:CDS 50.0%
! ATCTTGGGAAGCATGCCTAC+TGG + chr1_3:8727409-8727428 Msa0102020:CDS 50.0%
GTTGGCCAAGGTGAAAGAGT+TGG + chr1_3:8727570-8727589 Msa0102020:intron 50.0%
! CAAGGATGTGCAGCCTTTTG+AGG - chr1_3:8727717-8727736 None:intergenic 50.0%
CACCGATACAAGAGCCTTGA+TGG + chr1_3:8728001-8728020 Msa0102020:CDS 50.0%
GCCTTGATGGAGTTCAAAGC+TGG + chr1_3:8728014-8728033 Msa0102020:CDS 50.0%
ACCCAAAGTCAACCACCACG+TGG - chr1_3:8726843-8726862 None:intergenic 55.0%
CTTGGCAGTGTCAAAGGGGA+AGG + chr1_3:8727285-8727304 Msa0102020:CDS 55.0%
! TGGGAAGCATGCCTACTGGT+AGG + chr1_3:8727413-8727432 Msa0102020:intron 55.0%
ACCCAAAGTCAACCACCACG+TGG - chr1_3:8726843-8726862 None:intergenic 55.0%
CTTGGCAGTGTCAAAGGGGA+AGG + chr1_3:8727285-8727304 Msa0102020:CDS 55.0%
! TGGGAAGCATGCCTACTGGT+AGG + chr1_3:8727413-8727432 Msa0102020:intron 55.0%
CAGTGTCAAAGGGGAAGGCG+TGG + chr1_3:8727290-8727309 Msa0102020:CDS 60.0%
! CAAAGGGGAAGGCGTGGAGA+TGG + chr1_3:8727296-8727315 Msa0102020:CDS 60.0%
CAGTGTCAAAGGGGAAGGCG+TGG + chr1_3:8727290-8727309 Msa0102020:CDS 60.0%
! CAAAGGGGAAGGCGTGGAGA+TGG + chr1_3:8727296-8727315 Msa0102020:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 8726704 8728058 8726704 ID=Msa0102020;Name=Msa0102020
chr1_3 mRNA 8726704 8728058 8726704 ID=Msa0102020-mRNA-1;Parent=Msa0102020;Name=Msa0102020-mRNA-1;_AED=0.27;_eAED=0.37;_QI=306|0.5|0.66|1|0.5|0.66|3|0|110
chr1_3 exon 8726704 8727105 8726704 ID=Msa0102020-mRNA-1:exon:3497;Parent=Msa0102020-mRNA-1
chr1_3 exon 8727283 8727430 8727283 ID=Msa0102020-mRNA-1:exon:3498;Parent=Msa0102020-mRNA-1
chr1_3 exon 8727973 8728058 8727973 ID=Msa0102020-mRNA-1:exon:3499;Parent=Msa0102020-mRNA-1
chr1_3 five_prime_UTR 8726704 8727009 8726704 ID=Msa0102020-mRNA-1:five_prime_utr;Parent=Msa0102020-mRNA-1
chr1_3 CDS 8727010 8727105 8727010 ID=Msa0102020-mRNA-1:cds;Parent=Msa0102020-mRNA-1
chr1_3 CDS 8727283 8727430 8727283 ID=Msa0102020-mRNA-1:cds;Parent=Msa0102020-mRNA-1
chr1_3 CDS 8727973 8728058 8727973 ID=Msa0102020-mRNA-1:cds;Parent=Msa0102020-mRNA-1
Gene Sequence

>Msa0102020

ATGGGATCAACTCTACCAATTTCAAGAATCCACCATTTAGTTATAAGTATTCCTACAAAGATACTCATGGCCATTATGTTCACACAGGATGTTGATTTCTTGGCAGTGTCAAAGGGGAAGGCGTGGAGATGGATCTTTCTGTCGTGGTTGCGCAGAAGTTGCTGTGTTTGTCTGCATCCTGTTTTGCAAATAACAGTTTTTGGCCATTATAATTTTCTGGATATCTTGGGAAGCATGCCTACTGATGATGATGACATCAGGAGAAATGTGAACACCGATACAAGAGCCTTGATGGAGTTCAAAGCTGGAGTAAGGACTAAAATGAATCAA
Protein sequence

>Msa0102020

MGSTLPISRIHHLVISIPTKILMAIMFTQDVDFLAVSKGKAWRWIFLSWLRRSCCVCLHPVLQITVFGHYNFLDILGSMPTDDDDIRRNVNTDTRALMEFKAGVRTKMNQ