AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0114680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0114680 MtrunA17_Chr2g0303331 83.912 317 42 2 2 309 116 432 0.0 530
Msa0114680 MtrunA17_Chr4g0018601 52.431 288 109 10 1 272 94 369 1.68e-71 232
Msa0114680 MtrunA17_Chr4g0018601 72.727 121 22 3 158 267 498 618 5.11e-49 172
Msa0114680 MtrunA17_Chr1g0170751 56.571 175 38 6 158 306 474 636 2.93e-44 161
Msa0114680 MtrunA17_Chr1g0170751 40.234 256 107 8 33 272 120 345 9.80e-38 142
Msa0114680 MtrunA17_Chr2g0281961 58.462 65 11 2 11 75 97 145 3.23e-13 66.2
Msa0114680 MtrunA17_Chr1g0185901 37.500 120 54 4 189 293 582 695 7.03e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 98 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAGGAAATGTCAAATTT+TGG 0.102293 1_3:-32019953 Msa0114680:exon
CTTTGATTATTTAAATTTCT+AGG 0.188454 1_3:+32019232 None:intergenic
CATCCAACAAAGAAGGAAAA+AGG 0.225092 1_3:-32019166 Msa0114680:CDS
TTCTGTTGGTGCAGGATTGC+TGG 0.295279 1_3:-32019509 Msa0114680:intron
AATCGAACTGCCGAGGTTAA+AGG 0.304665 1_3:-32019644 Msa0114680:intron
ATTCAATACTATACACGAAT+TGG 0.317023 1_3:-32020014 Msa0114680:five_prime_UTR
GAGGATTTGAGTGGAGAAGC+TGG 0.330533 1_3:-32020195 Msa0114680:five_prime_UTR
TGGACTATGGAGGATGATGC+TGG 0.343721 1_3:-32020090 Msa0114680:five_prime_UTR
TGGCACTGACTCTTGGACTA+TGG 0.357408 1_3:-32020103 Msa0114680:five_prime_UTR
GGATAGAGTGGTTTCGGAAA+GGG 0.380946 1_3:-32020148 Msa0114680:five_prime_UTR
TTTAGAGGTATATATATTCA+TGG 0.383573 1_3:-32020298 Msa0114680:five_prime_UTR
TAATATTGTTGTTGATATCA+TGG 0.388013 1_3:-32019906 Msa0114680:CDS
TTCCTCAATATCAACAATAC+TGG 0.388085 1_3:+32019967 None:intergenic
TGCTGGGGCCGCAGGTCACC+CGG 0.388694 1_3:-32020073 Msa0114680:five_prime_UTR
TTGTTGGACTCCAGTTCATC+TGG 0.392081 1_3:-32019098 Msa0114680:CDS
GATGACTTCAAAATGCAAAC+TGG 0.393363 1_3:-32019028 Msa0114680:CDS
CTCCAGTATTGTTGATATTG+AGG 0.397240 1_3:-32019969 Msa0114680:five_prime_UTR
ACAAATTCATCGTTTACAAT+TGG 0.408584 1_3:-32018968 Msa0114680:CDS
TAAGTTGGATAGAGTGGTTT+CGG 0.414914 1_3:-32020154 Msa0114680:five_prime_UTR
TCAACTTTATCGTTCACAAT+TGG 0.425111 1_3:+32019857 None:intergenic
CAATAGTTGGCACTGACTCT+TGG 0.426514 1_3:-32020110 Msa0114680:five_prime_UTR
AATTGTAAACGATGAATTTG+TGG 0.437999 1_3:+32018970 None:intergenic
GCTGGGGCCGCAGGTCACCC+GGG 0.446878 1_3:-32020072 Msa0114680:five_prime_UTR
GAATCCAGAGAACCATTCAT+TGG 0.447830 1_3:+32019133 None:intergenic
ATTCTCCAGACGGTGATTGT+TGG 0.448044 1_3:-32019114 Msa0114680:intron
TCAGGTGAAACCAGATGAAC+TGG 0.448957 1_3:+32019088 None:intergenic
GAGGATGATGCTGGGGCCGC+AGG 0.449999 1_3:-32020081 Msa0114680:five_prime_UTR
CACCTGAACATTCCAAGAAA+AGG 0.455137 1_3:-32019072 Msa0114680:CDS
ATTGTGAACGATAAAGTTGA+TGG 0.456872 1_3:-32019854 Msa0114680:CDS
TGACGAGGAACAAGATTTGT+TGG 0.456916 1_3:-32019305 Msa0114680:CDS
AGGATTTGAGTGGAGAAGCT+GGG 0.462030 1_3:-32020194 Msa0114680:five_prime_UTR
AAAGCGTAAATATAATTTCA+AGG 0.466648 1_3:-32019197 Msa0114680:CDS
CGCAGTTAAGTTGGATAGAG+TGG 0.466901 1_3:-32020160 Msa0114680:five_prime_UTR
TGATCGACTGTATTAACAAC+AGG 0.467265 1_3:+32019262 None:intergenic
TGAGAATGCCGCAGTTAAGT+TGG 0.474499 1_3:-32020169 Msa0114680:five_prime_UTR
GAAACATCCAATATATTCTC+AGG 0.479638 1_3:+32018881 None:intergenic
GGACTATGGAGGATGATGCT+GGG 0.480835 1_3:-32020089 Msa0114680:five_prime_UTR
ATTGCGAATGAGTGATGAAA+GGG 0.481770 1_3:+32020567 None:intergenic
GCTAATGCAATTGTAGAAGA+AGG 0.499053 1_3:-32019457 Msa0114680:CDS
GGCACTCAAATTGCTGAGCA+AGG 0.502366 1_3:-32019436 Msa0114680:CDS
ATCTGATGTACGTGAGGGGA+TGG 0.502913 1_3:-32019714 Msa0114680:CDS
TGGATAGAGTGGTTTCGGAA+AGG 0.503876 1_3:-32020149 Msa0114680:five_prime_UTR
AGGATTGCTGGTATCTATTG+AGG 0.504951 1_3:-32019497 Msa0114680:CDS
TACTATGCATCCAACAAAGA+AGG 0.518171 1_3:-32019173 Msa0114680:CDS
GGAGTCAAATATGAGGCAAA+AGG 0.528193 1_3:-32019371 Msa0114680:CDS
AATCCAGAGAACCATTCATT+GGG 0.530099 1_3:+32019134 None:intergenic
GCGAGGTATCAATCAATAGT+TGG 0.532321 1_3:-32020123 Msa0114680:five_prime_UTR
ATCAACAACAATATTAGAGT+TGG 0.536272 1_3:+32019913 None:intergenic
AGATATCTTACCTTTAACCT+CGG 0.536941 1_3:+32019634 None:intergenic
AGGTATATGTCCACAGTTGT+TGG 0.550284 1_3:-32020261 Msa0114680:five_prime_UTR
GGATTGCTGGTATCTATTGA+GGG 0.552143 1_3:-32019496 Msa0114680:CDS
CAGATAGTTGAGGATTTGAG+TGG 0.552151 1_3:-32020204 Msa0114680:five_prime_UTR
CAATTGGTGACTGTATTCGT+AGG 0.553764 1_3:-32018952 Msa0114680:CDS
TCATGGAAGAAGTAGAAAAG+AGG 0.555897 1_3:-32020281 Msa0114680:five_prime_UTR
GAACTTCCACTCAACCTACC+TGG 0.557200 1_3:-32020512 Msa0114680:intron
GCTGGTATCTATTGAGGGCG+AGG 0.558742 1_3:-32019491 Msa0114680:CDS
GGGATGTCAGACCCAATGAA+TGG 0.563115 1_3:-32019145 Msa0114680:intron
TCAGCAAACAGTGATGTCCC+CGG 0.563331 1_3:+32020054 None:intergenic
GATTGCGAATGAGTGATGAA+AGG 0.568544 1_3:+32020566 None:intergenic
TGAGTGATGAAAGGGATTCG+AGG 0.570534 1_3:+32020575 None:intergenic
TCAACGAGGAGTCAAATATG+AGG 0.576367 1_3:-32019378 Msa0114680:CDS
ATGACTTCAAAATGCAAACT+GGG 0.578797 1_3:-32019027 Msa0114680:CDS
CTGGGGCCGCAGGTCACCCG+GGG 0.580762 1_3:-32020071 Msa0114680:five_prime_UTR
TGATGTCCCCGGGTGACCTG+CGG 0.582580 1_3:+32020065 None:intergenic
GTTAATACAGTCGATCAAGC+TGG 0.583440 1_3:-32019256 Msa0114680:CDS
CGTACTGTTGCATGATGGAA+AGG 0.584553 1_3:-32019407 Msa0114680:CDS
AAAGGGATTCGAGGTTTCAA+CGG 0.585390 1_3:+32020584 None:intergenic
ACATTCCACAGACTCGTCTA+CGG 0.587754 1_3:+32020233 None:intergenic
CAGCAAACAGTGATGTCCCC+GGG 0.587981 1_3:+32020055 None:intergenic
AATTGGTGACTGTATTCGTA+GGG 0.592472 1_3:-32018951 Msa0114680:CDS
TAGTTACCAGGTAGGTTGAG+TGG 0.593741 1_3:+32020506 None:intergenic
GACTATGGAGGATGATGCTG+GGG 0.595996 1_3:-32020088 Msa0114680:five_prime_UTR
CTATACACGAATTGGAATGA+CGG 0.608885 1_3:-32020006 Msa0114680:five_prime_UTR
AGCCACGTACTGTTGCATGA+TGG 0.612675 1_3:-32019412 Msa0114680:CDS
GAACAATCTGATGTACGTGA+GGG 0.614885 1_3:-32019719 Msa0114680:CDS
GGAGCAAGTAATAGCTGACA+AGG 0.615459 1_3:-32019771 Msa0114680:CDS
GCACTCAAATTGCTGAGCAA+GGG 0.615884 1_3:-32019435 Msa0114680:CDS
GTCATCATCAAAGTGATCGA+AGG 0.618361 1_3:+32019045 None:intergenic
AGAGCAAGAATGCGTTGACA+AGG 0.619110 1_3:-32019344 Msa0114680:CDS
CAGAAGACAGATGTAGACAT+TGG 0.619725 1_3:-32018911 Msa0114680:CDS
TTCCATCATGCAACAGTACG+TGG 0.628345 1_3:+32019410 None:intergenic
TGAACAATCTGATGTACGTG+AGG 0.634060 1_3:-32019720 Msa0114680:CDS
GAGTAATGTAGTTACCAGGT+AGG 0.634750 1_3:+32020498 None:intergenic
TACGGTTTCACCAACAACTG+TGG 0.637008 1_3:+32020251 None:intergenic
AGTTGAAAAGTGTAATGCAA+TGG 0.639577 1_3:-32019792 Msa0114680:CDS
ATTGTACATGAAGTTGTCAG+TGG 0.644697 1_3:-32019821 Msa0114680:CDS
CACTGACTCTTGGACTATGG+AGG 0.645014 1_3:-32020100 Msa0114680:five_prime_UTR
AACAATGAATCGAACTGCCG+AGG 0.648192 1_3:-32019651 Msa0114680:CDS
ATGTGGTGGTCAGATAGTTG+AGG 0.654622 1_3:-32020214 Msa0114680:five_prime_UTR
TGAAACCGTAGACGAGTCTG+TGG 0.661034 1_3:-32020238 Msa0114680:five_prime_UTR
CACTCTATCCAACTTAACTG+CGG 0.673432 1_3:+32020161 None:intergenic
TGGTTTCGGAAAGGGAAGCG+AGG 0.690904 1_3:-32020140 Msa0114680:five_prime_UTR
GACGAGTCTGTGGAATGTGG+TGG 0.693871 1_3:-32020228 Msa0114680:five_prime_UTR
AACAATCTGATGTACGTGAG+GGG 0.723105 1_3:-32019718 Msa0114680:CDS
AGTGGAAGTTCATTTCACAG+AGG 0.726866 1_3:+32020524 None:intergenic
ATTGATGTTGAAGATCAACA+AGG 0.730719 1_3:-32019689 Msa0114680:CDS
GTAGACGAGTCTGTGGAATG+TGG 0.748050 1_3:-32020231 Msa0114680:five_prime_UTR
ATAGCTGACAAGGATGTGAG+TGG 0.749474 1_3:-32019761 Msa0114680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTGATTATTTAAATTTCT+AGG + chr1_3:32020256-32020275 None:intergenic 15.0%
!! CTTTGATTATTTAAATTTCT+AGG + chr1_3:32020256-32020275 None:intergenic 15.0%
!!! ATTTAATTTGAATTTGAGCT+TGG - chr1_3:32019098-32019117 Msa0114680:CDS 20.0%
!!! AGATATAAAGAAAGTTTTAG+AGG - chr1_3:32019172-32019191 Msa0114680:CDS 20.0%
!!! TTTAGAGGTATATATATTCA+TGG - chr1_3:32019187-32019206 Msa0114680:CDS 20.0%
!!! TAATATTGTTGTTGATATCA+TGG - chr1_3:32019579-32019598 Msa0114680:intron 20.0%
!! AAAGCGTAAATATAATTTCA+AGG - chr1_3:32020288-32020307 Msa0114680:five_prime_UTR 20.0%
!!! ATTTAATTTGAATTTGAGCT+TGG - chr1_3:32019098-32019117 Msa0114680:CDS 20.0%
!!! AGATATAAAGAAAGTTTTAG+AGG - chr1_3:32019172-32019191 Msa0114680:CDS 20.0%
!!! TTTAGAGGTATATATATTCA+TGG - chr1_3:32019187-32019206 Msa0114680:CDS 20.0%
!!! TAATATTGTTGTTGATATCA+TGG - chr1_3:32019579-32019598 Msa0114680:intron 20.0%
!! AAAGCGTAAATATAATTTCA+AGG - chr1_3:32020288-32020307 Msa0114680:five_prime_UTR 20.0%
! TAGATTCAAACTATTGCATT+AGG - chr1_3:32019065-32019084 Msa0114680:CDS 25.0%
! AGATTCAAACTATTGCATTA+GGG - chr1_3:32019066-32019085 Msa0114680:CDS 25.0%
! ATTCAATACTATACACGAAT+TGG - chr1_3:32019471-32019490 Msa0114680:CDS 25.0%
!! ATTGAGGAAATGTCAAATTT+TGG - chr1_3:32019532-32019551 Msa0114680:intron 25.0%
!! TTGAGGAAATGTCAAATTTT+GGG - chr1_3:32019533-32019552 Msa0114680:intron 25.0%
! ATCAACAACAATATTAGAGT+TGG + chr1_3:32019575-32019594 None:intergenic 25.0%
! ATTTCAACTACTAATTAAGC+TGG + chr1_3:32019902-32019921 None:intergenic 25.0%
! AATTGTAAACGATGAATTTG+TGG + chr1_3:32020518-32020537 None:intergenic 25.0%
! ACAAATTCATCGTTTACAAT+TGG - chr1_3:32020517-32020536 Msa0114680:five_prime_UTR 25.0%
!! TGTTTTTCCTGAGAATATAT+TGG - chr1_3:32020597-32020616 Msa0114680:five_prime_UTR 25.0%
! TAGATTCAAACTATTGCATT+AGG - chr1_3:32019065-32019084 Msa0114680:CDS 25.0%
! AGATTCAAACTATTGCATTA+GGG - chr1_3:32019066-32019085 Msa0114680:CDS 25.0%
! ATTCAATACTATACACGAAT+TGG - chr1_3:32019471-32019490 Msa0114680:CDS 25.0%
!! ATTGAGGAAATGTCAAATTT+TGG - chr1_3:32019532-32019551 Msa0114680:intron 25.0%
!! TTGAGGAAATGTCAAATTTT+GGG - chr1_3:32019533-32019552 Msa0114680:intron 25.0%
! ATCAACAACAATATTAGAGT+TGG + chr1_3:32019575-32019594 None:intergenic 25.0%
! ATTTCAACTACTAATTAAGC+TGG + chr1_3:32019902-32019921 None:intergenic 25.0%
! AATTGTAAACGATGAATTTG+TGG + chr1_3:32020518-32020537 None:intergenic 25.0%
! ACAAATTCATCGTTTACAAT+TGG - chr1_3:32020517-32020536 Msa0114680:five_prime_UTR 25.0%
!! TGTTTTTCCTGAGAATATAT+TGG - chr1_3:32020597-32020616 Msa0114680:five_prime_UTR 25.0%
AATAGAGTAATGTAGTTACC+AGG + chr1_3:32018994-32019013 None:intergenic 30.0%
TTCCTCAATATCAACAATAC+TGG + chr1_3:32019521-32019540 None:intergenic 30.0%
TCAACTTTATCGTTCACAAT+TGG + chr1_3:32019631-32019650 None:intergenic 30.0%
ATTGTGAACGATAAAGTTGA+TGG - chr1_3:32019631-32019650 Msa0114680:intron 30.0%
! AGTTGAAAAGTGTAATGCAA+TGG - chr1_3:32019693-32019712 Msa0114680:CDS 30.0%
ATTGATGTTGAAGATCAACA+AGG - chr1_3:32019796-32019815 Msa0114680:CDS 30.0%
AGATATCTTACCTTTAACCT+CGG + chr1_3:32019854-32019873 None:intergenic 30.0%
!!! CAATTCATTTTTTGTTGACG+AGG - chr1_3:32020165-32020184 Msa0114680:five_prime_UTR 30.0%
ATCCAACAAAGAAGGAAAAA+GGG - chr1_3:32020320-32020339 Msa0114680:intron 30.0%
ATGACTTCAAAATGCAAACT+GGG - chr1_3:32020458-32020477 Msa0114680:intron 30.0%
AATAGAGTAATGTAGTTACC+AGG + chr1_3:32018994-32019013 None:intergenic 30.0%
TTCCTCAATATCAACAATAC+TGG + chr1_3:32019521-32019540 None:intergenic 30.0%
TCAACTTTATCGTTCACAAT+TGG + chr1_3:32019631-32019650 None:intergenic 30.0%
ATTGTGAACGATAAAGTTGA+TGG - chr1_3:32019631-32019650 Msa0114680:intron 30.0%
! AGTTGAAAAGTGTAATGCAA+TGG - chr1_3:32019693-32019712 Msa0114680:CDS 30.0%
ATTGATGTTGAAGATCAACA+AGG - chr1_3:32019796-32019815 Msa0114680:CDS 30.0%
AGATATCTTACCTTTAACCT+CGG + chr1_3:32019854-32019873 None:intergenic 30.0%
!!! CAATTCATTTTTTGTTGACG+AGG - chr1_3:32020165-32020184 Msa0114680:five_prime_UTR 30.0%
ATCCAACAAAGAAGGAAAAA+GGG - chr1_3:32020320-32020339 Msa0114680:intron 30.0%
ATGACTTCAAAATGCAAACT+GGG - chr1_3:32020458-32020477 Msa0114680:intron 30.0%
ATTGCGAATGAGTGATGAAA+GGG + chr1_3:32018921-32018940 None:intergenic 35.0%
TCATGGAAGAAGTAGAAAAG+AGG - chr1_3:32019204-32019223 Msa0114680:CDS 35.0%
! TAAGTTGGATAGAGTGGTTT+CGG - chr1_3:32019331-32019350 Msa0114680:CDS 35.0%
CTATACACGAATTGGAATGA+CGG - chr1_3:32019479-32019498 Msa0114680:CDS 35.0%
CTCCAGTATTGTTGATATTG+AGG - chr1_3:32019516-32019535 Msa0114680:intron 35.0%
ATTGTACATGAAGTTGTCAG+TGG - chr1_3:32019664-32019683 Msa0114680:CDS 35.0%
AAGATATCTGCTGATCTCAT+TGG - chr1_3:32019865-32019884 Msa0114680:CDS 35.0%
GCTAATGCAATTGTAGAAGA+AGG - chr1_3:32020028-32020047 Msa0114680:five_prime_UTR 35.0%
TGATCGACTGTATTAACAAC+AGG + chr1_3:32020226-32020245 None:intergenic 35.0%
TACTATGCATCCAACAAAGA+AGG - chr1_3:32020312-32020331 Msa0114680:five_prime_UTR 35.0%
CATCCAACAAAGAAGGAAAA+AGG - chr1_3:32020319-32020338 Msa0114680:intron 35.0%
! ATCCCTTTTTCCTTCTTTGT+TGG + chr1_3:32020325-32020344 None:intergenic 35.0%
AATCCAGAGAACCATTCATT+GGG + chr1_3:32020354-32020373 None:intergenic 35.0%
GATGACTTCAAAATGCAAAC+TGG - chr1_3:32020457-32020476 Msa0114680:intron 35.0%
! AATTGGTGACTGTATTCGTA+GGG - chr1_3:32020534-32020553 Msa0114680:five_prime_UTR 35.0%
ATTGCGAATGAGTGATGAAA+GGG + chr1_3:32018921-32018940 None:intergenic 35.0%
TCATGGAAGAAGTAGAAAAG+AGG - chr1_3:32019204-32019223 Msa0114680:CDS 35.0%
! TAAGTTGGATAGAGTGGTTT+CGG - chr1_3:32019331-32019350 Msa0114680:CDS 35.0%
CTATACACGAATTGGAATGA+CGG - chr1_3:32019479-32019498 Msa0114680:CDS 35.0%
CTCCAGTATTGTTGATATTG+AGG - chr1_3:32019516-32019535 Msa0114680:intron 35.0%
ATTGTACATGAAGTTGTCAG+TGG - chr1_3:32019664-32019683 Msa0114680:CDS 35.0%
AAGATATCTGCTGATCTCAT+TGG - chr1_3:32019865-32019884 Msa0114680:CDS 35.0%
GCTAATGCAATTGTAGAAGA+AGG - chr1_3:32020028-32020047 Msa0114680:five_prime_UTR 35.0%
TGATCGACTGTATTAACAAC+AGG + chr1_3:32020226-32020245 None:intergenic 35.0%
TACTATGCATCCAACAAAGA+AGG - chr1_3:32020312-32020331 Msa0114680:five_prime_UTR 35.0%
CATCCAACAAAGAAGGAAAA+AGG - chr1_3:32020319-32020338 Msa0114680:intron 35.0%
! ATCCCTTTTTCCTTCTTTGT+TGG + chr1_3:32020325-32020344 None:intergenic 35.0%
AATCCAGAGAACCATTCATT+GGG + chr1_3:32020354-32020373 None:intergenic 35.0%
GATGACTTCAAAATGCAAAC+TGG - chr1_3:32020457-32020476 Msa0114680:intron 35.0%
! AATTGGTGACTGTATTCGTA+GGG - chr1_3:32020534-32020553 Msa0114680:five_prime_UTR 35.0%
AAAGGGATTCGAGGTTTCAA+CGG + chr1_3:32018904-32018923 None:intergenic 40.0%
GATTGCGAATGAGTGATGAA+AGG + chr1_3:32018922-32018941 None:intergenic 40.0%
AGTGGAAGTTCATTTCACAG+AGG + chr1_3:32018964-32018983 None:intergenic 40.0%
GAGTAATGTAGTTACCAGGT+AGG + chr1_3:32018990-32019009 None:intergenic 40.0%
AGGTATATGTCCACAGTTGT+TGG - chr1_3:32019224-32019243 Msa0114680:CDS 40.0%
CAGATAGTTGAGGATTTGAG+TGG - chr1_3:32019281-32019300 Msa0114680:intron 40.0%
CACTCTATCCAACTTAACTG+CGG + chr1_3:32019327-32019346 None:intergenic 40.0%
! GCGAGGTATCAATCAATAGT+TGG - chr1_3:32019362-32019381 Msa0114680:CDS 40.0%
TGAACAATCTGATGTACGTG+AGG - chr1_3:32019765-32019784 Msa0114680:CDS 40.0%
GAACAATCTGATGTACGTGA+GGG - chr1_3:32019766-32019785 Msa0114680:CDS 40.0%
AACAATCTGATGTACGTGAG+GGG - chr1_3:32019767-32019786 Msa0114680:CDS 40.0%
! AGTGATGCTTGTAGTTCTGT+TGG - chr1_3:32019962-32019981 Msa0114680:exon 40.0%
AGGATTGCTGGTATCTATTG+AGG - chr1_3:32019988-32020007 Msa0114680:five_prime_UTR 40.0%
GGATTGCTGGTATCTATTGA+GGG - chr1_3:32019989-32020008 Msa0114680:five_prime_UTR 40.0%
TGGAAAGGAAAAACTCAACG+AGG - chr1_3:32020093-32020112 Msa0114680:five_prime_UTR 40.0%
TCAACGAGGAGTCAAATATG+AGG - chr1_3:32020107-32020126 Msa0114680:five_prime_UTR 40.0%
GGAGTCAAATATGAGGCAAA+AGG - chr1_3:32020114-32020133 Msa0114680:five_prime_UTR 40.0%
TGACGAGGAACAAGATTTGT+TGG - chr1_3:32020180-32020199 Msa0114680:five_prime_UTR 40.0%
GTTAATACAGTCGATCAAGC+TGG - chr1_3:32020229-32020248 Msa0114680:five_prime_UTR 40.0%
GAATCCAGAGAACCATTCAT+TGG + chr1_3:32020355-32020374 None:intergenic 40.0%
CACCTGAACATTCCAAGAAA+AGG - chr1_3:32020413-32020432 Msa0114680:intron 40.0%
! CTCCTTTTCTTGGAATGTTC+AGG + chr1_3:32020418-32020437 None:intergenic 40.0%
GTCATCATCAAAGTGATCGA+AGG + chr1_3:32020443-32020462 None:intergenic 40.0%
! CAATTGGTGACTGTATTCGT+AGG - chr1_3:32020533-32020552 Msa0114680:five_prime_UTR 40.0%
CAGAAGACAGATGTAGACAT+TGG - chr1_3:32020574-32020593 Msa0114680:five_prime_UTR 40.0%
AAAGGGATTCGAGGTTTCAA+CGG + chr1_3:32018904-32018923 None:intergenic 40.0%
GATTGCGAATGAGTGATGAA+AGG + chr1_3:32018922-32018941 None:intergenic 40.0%
AGTGGAAGTTCATTTCACAG+AGG + chr1_3:32018964-32018983 None:intergenic 40.0%
GAGTAATGTAGTTACCAGGT+AGG + chr1_3:32018990-32019009 None:intergenic 40.0%
AGGTATATGTCCACAGTTGT+TGG - chr1_3:32019224-32019243 Msa0114680:CDS 40.0%
CAGATAGTTGAGGATTTGAG+TGG - chr1_3:32019281-32019300 Msa0114680:intron 40.0%
CACTCTATCCAACTTAACTG+CGG + chr1_3:32019327-32019346 None:intergenic 40.0%
! GCGAGGTATCAATCAATAGT+TGG - chr1_3:32019362-32019381 Msa0114680:CDS 40.0%
TGAACAATCTGATGTACGTG+AGG - chr1_3:32019765-32019784 Msa0114680:CDS 40.0%
GAACAATCTGATGTACGTGA+GGG - chr1_3:32019766-32019785 Msa0114680:CDS 40.0%
AACAATCTGATGTACGTGAG+GGG - chr1_3:32019767-32019786 Msa0114680:CDS 40.0%
! AGTGATGCTTGTAGTTCTGT+TGG - chr1_3:32019962-32019981 Msa0114680:exon 40.0%
AGGATTGCTGGTATCTATTG+AGG - chr1_3:32019988-32020007 Msa0114680:five_prime_UTR 40.0%
GGATTGCTGGTATCTATTGA+GGG - chr1_3:32019989-32020008 Msa0114680:five_prime_UTR 40.0%
TGGAAAGGAAAAACTCAACG+AGG - chr1_3:32020093-32020112 Msa0114680:five_prime_UTR 40.0%
TCAACGAGGAGTCAAATATG+AGG - chr1_3:32020107-32020126 Msa0114680:five_prime_UTR 40.0%
GGAGTCAAATATGAGGCAAA+AGG - chr1_3:32020114-32020133 Msa0114680:five_prime_UTR 40.0%
TGACGAGGAACAAGATTTGT+TGG - chr1_3:32020180-32020199 Msa0114680:five_prime_UTR 40.0%
GTTAATACAGTCGATCAAGC+TGG - chr1_3:32020229-32020248 Msa0114680:five_prime_UTR 40.0%
GAATCCAGAGAACCATTCAT+TGG + chr1_3:32020355-32020374 None:intergenic 40.0%
CACCTGAACATTCCAAGAAA+AGG - chr1_3:32020413-32020432 Msa0114680:intron 40.0%
! CTCCTTTTCTTGGAATGTTC+AGG + chr1_3:32020418-32020437 None:intergenic 40.0%
GTCATCATCAAAGTGATCGA+AGG + chr1_3:32020443-32020462 None:intergenic 40.0%
! CAATTGGTGACTGTATTCGT+AGG - chr1_3:32020533-32020552 Msa0114680:five_prime_UTR 40.0%
CAGAAGACAGATGTAGACAT+TGG - chr1_3:32020574-32020593 Msa0114680:five_prime_UTR 40.0%
TGAGTGATGAAAGGGATTCG+AGG + chr1_3:32018913-32018932 None:intergenic 45.0%
! TAGTTACCAGGTAGGTTGAG+TGG + chr1_3:32018982-32019001 None:intergenic 45.0%
TACGGTTTCACCAACAACTG+TGG + chr1_3:32019237-32019256 None:intergenic 45.0%
ACATTCCACAGACTCGTCTA+CGG + chr1_3:32019255-32019274 None:intergenic 45.0%
ATGTGGTGGTCAGATAGTTG+AGG - chr1_3:32019271-32019290 Msa0114680:CDS 45.0%
AGGATTTGAGTGGAGAAGCT+GGG - chr1_3:32019291-32019310 Msa0114680:intron 45.0%
TGAGAATGCCGCAGTTAAGT+TGG - chr1_3:32019316-32019335 Msa0114680:CDS 45.0%
!! CGCAGTTAAGTTGGATAGAG+TGG - chr1_3:32019325-32019344 Msa0114680:CDS 45.0%
TGGATAGAGTGGTTTCGGAA+AGG - chr1_3:32019336-32019355 Msa0114680:CDS 45.0%
GGATAGAGTGGTTTCGGAAA+GGG - chr1_3:32019337-32019356 Msa0114680:CDS 45.0%
!! CAATAGTTGGCACTGACTCT+TGG - chr1_3:32019375-32019394 Msa0114680:CDS 45.0%
GGAGCAAGTAATAGCTGACA+AGG - chr1_3:32019714-32019733 Msa0114680:CDS 45.0%
ATAGCTGACAAGGATGTGAG+TGG - chr1_3:32019724-32019743 Msa0114680:CDS 45.0%
AACAATGAATCGAACTGCCG+AGG - chr1_3:32019834-32019853 Msa0114680:CDS 45.0%
AATCGAACTGCCGAGGTTAA+AGG - chr1_3:32019841-32019860 Msa0114680:CDS 45.0%
GCACTCAAATTGCTGAGCAA+GGG - chr1_3:32020050-32020069 Msa0114680:five_prime_UTR 45.0%
TTCCATCATGCAACAGTACG+TGG + chr1_3:32020078-32020097 None:intergenic 45.0%
! CGTACTGTTGCATGATGGAA+AGG - chr1_3:32020078-32020097 Msa0114680:five_prime_UTR 45.0%
! AGAGCAAGAATGCGTTGACA+AGG - chr1_3:32020141-32020160 Msa0114680:five_prime_UTR 45.0%
AGACCCAATGAATGGTTCTC+TGG - chr1_3:32020348-32020367 Msa0114680:intron 45.0%
ATTCTCCAGACGGTGATTGT+TGG - chr1_3:32020371-32020390 Msa0114680:intron 45.0%
TTGTTGGACTCCAGTTCATC+TGG - chr1_3:32020387-32020406 Msa0114680:intron 45.0%
TCAGGTGAAACCAGATGAAC+TGG + chr1_3:32020400-32020419 None:intergenic 45.0%
!!! ATCGAAGGTGCTCCTTTTCT+TGG + chr1_3:32020428-32020447 None:intergenic 45.0%
TGAGTGATGAAAGGGATTCG+AGG + chr1_3:32018913-32018932 None:intergenic 45.0%
! TAGTTACCAGGTAGGTTGAG+TGG + chr1_3:32018982-32019001 None:intergenic 45.0%
TACGGTTTCACCAACAACTG+TGG + chr1_3:32019237-32019256 None:intergenic 45.0%
ACATTCCACAGACTCGTCTA+CGG + chr1_3:32019255-32019274 None:intergenic 45.0%
ATGTGGTGGTCAGATAGTTG+AGG - chr1_3:32019271-32019290 Msa0114680:CDS 45.0%
AGGATTTGAGTGGAGAAGCT+GGG - chr1_3:32019291-32019310 Msa0114680:intron 45.0%
TGAGAATGCCGCAGTTAAGT+TGG - chr1_3:32019316-32019335 Msa0114680:CDS 45.0%
!! CGCAGTTAAGTTGGATAGAG+TGG - chr1_3:32019325-32019344 Msa0114680:CDS 45.0%
TGGATAGAGTGGTTTCGGAA+AGG - chr1_3:32019336-32019355 Msa0114680:CDS 45.0%
GGATAGAGTGGTTTCGGAAA+GGG - chr1_3:32019337-32019356 Msa0114680:CDS 45.0%
!! CAATAGTTGGCACTGACTCT+TGG - chr1_3:32019375-32019394 Msa0114680:CDS 45.0%
GGAGCAAGTAATAGCTGACA+AGG - chr1_3:32019714-32019733 Msa0114680:CDS 45.0%
ATAGCTGACAAGGATGTGAG+TGG - chr1_3:32019724-32019743 Msa0114680:CDS 45.0%
AACAATGAATCGAACTGCCG+AGG - chr1_3:32019834-32019853 Msa0114680:CDS 45.0%
AATCGAACTGCCGAGGTTAA+AGG - chr1_3:32019841-32019860 Msa0114680:CDS 45.0%
GCACTCAAATTGCTGAGCAA+GGG - chr1_3:32020050-32020069 Msa0114680:five_prime_UTR 45.0%
TTCCATCATGCAACAGTACG+TGG + chr1_3:32020078-32020097 None:intergenic 45.0%
! CGTACTGTTGCATGATGGAA+AGG - chr1_3:32020078-32020097 Msa0114680:five_prime_UTR 45.0%
! AGAGCAAGAATGCGTTGACA+AGG - chr1_3:32020141-32020160 Msa0114680:five_prime_UTR 45.0%
AGACCCAATGAATGGTTCTC+TGG - chr1_3:32020348-32020367 Msa0114680:intron 45.0%
ATTCTCCAGACGGTGATTGT+TGG - chr1_3:32020371-32020390 Msa0114680:intron 45.0%
TTGTTGGACTCCAGTTCATC+TGG - chr1_3:32020387-32020406 Msa0114680:intron 45.0%
TCAGGTGAAACCAGATGAAC+TGG + chr1_3:32020400-32020419 None:intergenic 45.0%
!!! ATCGAAGGTGCTCCTTTTCT+TGG + chr1_3:32020428-32020447 None:intergenic 45.0%
GAACTTCCACTCAACCTACC+TGG - chr1_3:32018973-32018992 Msa0114680:CDS 50.0%
TGAAACCGTAGACGAGTCTG+TGG - chr1_3:32019247-32019266 Msa0114680:CDS 50.0%
GTAGACGAGTCTGTGGAATG+TGG - chr1_3:32019254-32019273 Msa0114680:CDS 50.0%
GAGGATTTGAGTGGAGAAGC+TGG - chr1_3:32019290-32019309 Msa0114680:intron 50.0%
!! TGGCACTGACTCTTGGACTA+TGG - chr1_3:32019382-32019401 Msa0114680:CDS 50.0%
!! CACTGACTCTTGGACTATGG+AGG - chr1_3:32019385-32019404 Msa0114680:CDS 50.0%
TGGACTATGGAGGATGATGC+TGG - chr1_3:32019395-32019414 Msa0114680:CDS 50.0%
GGACTATGGAGGATGATGCT+GGG - chr1_3:32019396-32019415 Msa0114680:CDS 50.0%
GACTATGGAGGATGATGCTG+GGG - chr1_3:32019397-32019416 Msa0114680:CDS 50.0%
TCAGCAAACAGTGATGTCCC+CGG + chr1_3:32019434-32019453 None:intergenic 50.0%
ATCTGATGTACGTGAGGGGA+TGG - chr1_3:32019771-32019790 Msa0114680:CDS 50.0%
! GCTTGTAGTTCTGTTGGTGC+AGG - chr1_3:32019968-32019987 Msa0114680:five_prime_UTR 50.0%
TTCTGTTGGTGCAGGATTGC+TGG - chr1_3:32019976-32019995 Msa0114680:five_prime_UTR 50.0%
GGCACTCAAATTGCTGAGCA+AGG - chr1_3:32020049-32020068 Msa0114680:five_prime_UTR 50.0%
AGCCACGTACTGTTGCATGA+TGG - chr1_3:32020073-32020092 Msa0114680:five_prime_UTR 50.0%
GGGATGTCAGACCCAATGAA+TGG - chr1_3:32020340-32020359 Msa0114680:intron 50.0%
GGTTCTCTGGATTCTCCAGA+CGG - chr1_3:32020361-32020380 Msa0114680:intron 50.0%
GAACTTCCACTCAACCTACC+TGG - chr1_3:32018973-32018992 Msa0114680:CDS 50.0%
TGAAACCGTAGACGAGTCTG+TGG - chr1_3:32019247-32019266 Msa0114680:CDS 50.0%
GTAGACGAGTCTGTGGAATG+TGG - chr1_3:32019254-32019273 Msa0114680:CDS 50.0%
GAGGATTTGAGTGGAGAAGC+TGG - chr1_3:32019290-32019309 Msa0114680:intron 50.0%
!! TGGCACTGACTCTTGGACTA+TGG - chr1_3:32019382-32019401 Msa0114680:CDS 50.0%
!! CACTGACTCTTGGACTATGG+AGG - chr1_3:32019385-32019404 Msa0114680:CDS 50.0%
TGGACTATGGAGGATGATGC+TGG - chr1_3:32019395-32019414 Msa0114680:CDS 50.0%
GGACTATGGAGGATGATGCT+GGG - chr1_3:32019396-32019415 Msa0114680:CDS 50.0%
GACTATGGAGGATGATGCTG+GGG - chr1_3:32019397-32019416 Msa0114680:CDS 50.0%
TCAGCAAACAGTGATGTCCC+CGG + chr1_3:32019434-32019453 None:intergenic 50.0%
ATCTGATGTACGTGAGGGGA+TGG - chr1_3:32019771-32019790 Msa0114680:CDS 50.0%
! GCTTGTAGTTCTGTTGGTGC+AGG - chr1_3:32019968-32019987 Msa0114680:five_prime_UTR 50.0%
TTCTGTTGGTGCAGGATTGC+TGG - chr1_3:32019976-32019995 Msa0114680:five_prime_UTR 50.0%
GGCACTCAAATTGCTGAGCA+AGG - chr1_3:32020049-32020068 Msa0114680:five_prime_UTR 50.0%
AGCCACGTACTGTTGCATGA+TGG - chr1_3:32020073-32020092 Msa0114680:five_prime_UTR 50.0%
GGGATGTCAGACCCAATGAA+TGG - chr1_3:32020340-32020359 Msa0114680:intron 50.0%
GGTTCTCTGGATTCTCCAGA+CGG - chr1_3:32020361-32020380 Msa0114680:intron 50.0%
GACGAGTCTGTGGAATGTGG+TGG - chr1_3:32019257-32019276 Msa0114680:CDS 55.0%
TGGTTTCGGAAAGGGAAGCG+AGG - chr1_3:32019345-32019364 Msa0114680:CDS 55.0%
CAGCAAACAGTGATGTCCCC+GGG + chr1_3:32019433-32019452 None:intergenic 55.0%
GCTGGTATCTATTGAGGGCG+AGG - chr1_3:32019994-32020013 Msa0114680:five_prime_UTR 55.0%
GGAGTCCAACAATCACCGTC+TGG + chr1_3:32020379-32020398 None:intergenic 55.0%
GACGAGTCTGTGGAATGTGG+TGG - chr1_3:32019257-32019276 Msa0114680:CDS 55.0%
TGGTTTCGGAAAGGGAAGCG+AGG - chr1_3:32019345-32019364 Msa0114680:CDS 55.0%
CAGCAAACAGTGATGTCCCC+GGG + chr1_3:32019433-32019452 None:intergenic 55.0%
GCTGGTATCTATTGAGGGCG+AGG - chr1_3:32019994-32020013 Msa0114680:five_prime_UTR 55.0%
GGAGTCCAACAATCACCGTC+TGG + chr1_3:32020379-32020398 None:intergenic 55.0%
TGATGTCCCCGGGTGACCTG+CGG + chr1_3:32019423-32019442 None:intergenic 65.0%
TGATGTCCCCGGGTGACCTG+CGG + chr1_3:32019423-32019442 None:intergenic 65.0%
GAGGATGATGCTGGGGCCGC+AGG - chr1_3:32019404-32019423 Msa0114680:CDS 70.0%
GAGGATGATGCTGGGGCCGC+AGG - chr1_3:32019404-32019423 Msa0114680:CDS 70.0%
TGCTGGGGCCGCAGGTCACC+CGG - chr1_3:32019412-32019431 Msa0114680:CDS 75.0%
TGCTGGGGCCGCAGGTCACC+CGG - chr1_3:32019412-32019431 Msa0114680:CDS 75.0%
! GCTGGGGCCGCAGGTCACCC+GGG - chr1_3:32019413-32019432 Msa0114680:CDS 80.0%
! CTGGGGCCGCAGGTCACCCG+GGG - chr1_3:32019414-32019433 Msa0114680:CDS 80.0%
! GCTGGGGCCGCAGGTCACCC+GGG - chr1_3:32019413-32019432 Msa0114680:CDS 80.0%
! CTGGGGCCGCAGGTCACCCG+GGG - chr1_3:32019414-32019433 Msa0114680:CDS 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 32018886 32020621 32018886 ID=Msa0114680;Name=Msa0114680
chr1_3 mRNA 32018886 32020621 32018886 ID=Msa0114680-mRNA-1;Parent=Msa0114680;Name=Msa0114680-mRNA-1;_AED=0.03;_eAED=0.03;_QI=476|0.5|0.4|1|0.25|0|5|0|309
chr1_3 exon 32018886 32019127 32018886 ID=Msa0114680-mRNA-1:exon:10371;Parent=Msa0114680-mRNA-1
chr1_3 exon 32019146 32019293 32019146 ID=Msa0114680-mRNA-1:exon:10370;Parent=Msa0114680-mRNA-1
chr1_3 exon 32019303 32019517 32019303 ID=Msa0114680-mRNA-1:exon:10369;Parent=Msa0114680-mRNA-1
chr1_3 exon 32019645 32020333 32019645 ID=Msa0114680-mRNA-1:exon:10368;Parent=Msa0114680-mRNA-1
chr1_3 exon 32020513 32020621 32020513 ID=Msa0114680-mRNA-1:exon:10367;Parent=Msa0114680-mRNA-1
chr1_3 five_prime_UTR 32020513 32020621 32020513 ID=Msa0114680-mRNA-1:five_prime_utr;Parent=Msa0114680-mRNA-1
chr1_3 five_prime_UTR 32019967 32020333 32019967 ID=Msa0114680-mRNA-1:five_prime_utr;Parent=Msa0114680-mRNA-1
chr1_3 CDS 32019645 32019966 32019645 ID=Msa0114680-mRNA-1:cds;Parent=Msa0114680-mRNA-1
chr1_3 CDS 32019303 32019517 32019303 ID=Msa0114680-mRNA-1:cds;Parent=Msa0114680-mRNA-1
chr1_3 CDS 32019146 32019293 32019146 ID=Msa0114680-mRNA-1:cds;Parent=Msa0114680-mRNA-1
chr1_3 CDS 32018886 32019127 32018886 ID=Msa0114680-mRNA-1:cds;Parent=Msa0114680-mRNA-1
Gene Sequence

>Msa0114680

ATGTCAAATTTTGGGAATGATAAATTTGATGCCAACTCTAATATTGTTGTTGATATCATGGAAACGATAGATTCAATATTGAATATGCCAATTGTGAACGATAAAGTTGATGGCAGAGCAAGTATTGTACATGAAGTTGTCAGTGGAACTGAAGTTGAAAAGTGTAATGCAATGGAGCAAGTAATAGCTGACAAGGATGTGAGTGGTGTTTCTGCTTTGAATACTGAACAATCTGATGTACGTGAGGGGATGGAAATTGATGTTGAAGATCAACAAGGAATAGAAAGAAGTCGAACAATGAATCGAACTGCCGAGGTTAAAGGATTGCTGGTATCTATTGAGGGCGAGGAAAATTTTCATGCTAATGCAATTGTAGAAGAAGGCACTCAAATTGCTGAGCAAGGGAGCCACGTACTGTTGCATGATGGAAAGGAAAAACTCAACGAGGAGTCAAATATGAGGCAAAAGGTTGAAGAGCAAGAATGCGTTGACAAGGACAATTCATTTTTTGTTGACGAGGAACAAGATTTGTTGGAATATGCAACTCCTGTTGTTAATACAGTCGATCAAGCTGGTCCTAGAAATTTAAATAATCAAAGCAGCGCGCATCAAAAGCGTAAATATAATTTCAAGGATACTATGCATCCAACAAAGAAGGAAAAAGGGATGTCAGACCCAATGAATGACGGTGATTGTTGGACTCCAGTTCATCTGGTTTCACCTGAACATTCCAAGAAAAGGAGCACCTTCGATCACTTTGATGATGACTTCAAAATGCAAACTGGGAAAAAAACAATTTCTGTTGCAGAAGTTTCAAATACCACAAATTCATCGTTTACAATTGGTGACTGTATTCGTAGGGCTGATAGTCAACTCACTCAGAAGACAGATGTAGACATTGGTGTTTTTCCTGAGAATATATTGGAT
Protein sequence

>Msa0114680

MSNFGNDKFDANSNIVVDIMETIDSILNMPIVNDKVDGRASIVHEVVSGTEVEKCNAMEQVIADKDVSGVSALNTEQSDVREGMEIDVEDQQGIERSRTMNRTAEVKGLLVSIEGEENFHANAIVEEGTQIAEQGSHVLLHDGKEKLNEESNMRQKVEEQECVDKDNSFFVDEEQDLLEYATPVVNTVDQAGPRNLNNQSSAHQKRKYNFKDTMHPTKKEKGMSDPMNDGDCWTPVHLVSPEHSKKRSTFDHFDDDFKMQTGKKTISVAEVSNTTNSSFTIGDCIRRADSQLTQKTDVDIGVFPENILD