AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128810 MtrunA17_Chr1g0195661 96.471 170 3 1 1 170 1 167 1.16e-117 329
Msa0128810 MtrunA17_Chr7g0269301 68.590 156 46 2 16 170 9 162 2.35e-66 199
Msa0128810 MtrunA17_Chr1g0194801 40.252 159 90 3 11 166 2 158 2.82e-34 118
Msa0128810 MtrunA17_Chr1g0176321 40.462 173 79 5 18 170 6 174 4.05e-33 115
Msa0128810 MtrunA17_Chr1g0192651 39.752 161 89 4 10 168 5 159 1.32e-32 114
Msa0128810 MtrunA17_Chr1g0176311 37.805 164 90 3 18 170 25 187 1.06e-30 110
Msa0128810 MtrunA17_Chr5g0427291 32.716 162 96 3 16 164 9 170 3.41e-22 87.8
Msa0128810 MtrunA17_Chr2g0323291 29.560 159 99 4 19 167 29 184 9.96e-16 72.0
Msa0128810 MtrunA17_Chr4g0001641 49.206 63 32 0 102 164 3 65 1.02e-15 68.2
Msa0128810 MtrunA17_Chr4g0032261 32.258 155 92 4 19 165 9 158 1.31e-15 70.5
Msa0128810 MtrunA17_Chr6g0450171 24.051 158 109 3 17 165 8 163 1.19e-12 62.8
Msa0128810 MtrunA17_Chr5g0433951 29.032 155 95 5 19 164 9 157 1.51e-12 62.4
Msa0128810 MtrunA17_Chr2g0294421 29.375 160 100 5 19 170 9 163 2.75e-12 61.6
Msa0128810 MtrunA17_Chr7g0240701 29.936 157 91 6 19 164 9 157 1.09e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128810 AT2G47710.1 62.195 164 57 2 8 169 1 161 2.11e-70 210
Msa0128810 AT3G11930.1 40.449 178 87 6 7 167 20 195 7.54e-34 119
Msa0128810 AT3G11930.2 40.223 179 87 6 7 167 20 196 1.06e-33 118
Msa0128810 AT3G11930.4 40.000 180 87 6 7 167 20 197 1.35e-33 118
Msa0128810 AT1G09740.2 38.365 159 88 5 17 167 10 166 3.67e-28 103
Msa0128810 AT1G09740.1 38.365 159 88 5 17 167 10 166 3.67e-28 103
Msa0128810 AT3G11930.3 35.096 208 83 8 7 167 20 222 1.11e-27 103
Msa0128810 AT3G58450.1 37.349 166 83 5 17 167 32 191 4.93e-26 99.0
Msa0128810 AT3G62550.1 36.905 168 85 8 7 165 2 157 1.36e-25 96.7
Msa0128810 AT3G58450.2 36.145 166 78 6 17 167 32 184 4.90e-25 95.9
Msa0128810 AT5G49050.1 42.735 117 62 2 13 127 37 150 2.31e-24 93.2
Msa0128810 AT1G11360.2 32.571 175 94 6 10 169 33 198 9.28e-19 80.5
Msa0128810 AT1G11360.1 32.571 175 94 6 10 169 33 198 9.28e-19 80.5
Msa0128810 AT1G11360.4 32.571 175 94 6 10 169 33 198 9.28e-19 80.5
Msa0128810 AT1G11360.3 32.571 175 94 6 10 169 33 198 9.28e-19 80.5
Msa0128810 AT1G68300.1 32.278 158 100 4 11 164 3 157 2.76e-18 77.4
Msa0128810 AT5G14680.1 27.647 170 99 5 12 165 2 163 3.76e-16 72.4
Msa0128810 AT3G01520.1 28.676 136 95 2 30 165 30 163 7.29e-15 68.9
Msa0128810 AT3G17020.1 28.477 151 102 2 19 164 11 160 8.54e-13 63.2
Msa0128810 AT3G53990.1 25.325 154 102 4 19 164 9 157 2.82e-12 61.6
Msa0128810 AT2G21620.1 29.932 147 80 4 17 163 42 165 2.61e-11 59.7
Msa0128810 AT2G21620.2 30.612 147 85 4 17 163 42 171 2.68e-11 59.7

Find 41 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTGATACTTTATGAAAA+TGG 0.199567 1_3:+58647994 Msa0128810:three_prime_UTR
TTCATGTACAAAGTAAAATA+AGG 0.287810 1_3:-58647913 None:intergenic
TTGTAGGTAGTCACGGTTAT+GGG 0.301582 1_3:+58647148 Msa0128810:CDS
AGTTTATCCTATGGCATGTT+TGG 0.308645 1_3:+58647829 Msa0128810:three_prime_UTR
TCGATTGCAACTAACATTAC+TGG 0.334213 1_3:-58645944 None:intergenic
AACCAAGCACTGATCTATAT+TGG 0.359536 1_3:+58647731 Msa0128810:exon
GTTGTAGGTAGTCACGGTTA+TGG 0.361010 1_3:+58647147 Msa0128810:CDS
CGTGCTTCAATCTTGGTTGT+AGG 0.387207 1_3:+58647132 Msa0128810:CDS
CTCAAATCAGAATCCACAAT+TGG 0.390759 1_3:-58646213 None:intergenic
AAAGTATCGTGCTTCAATCT+TGG 0.402940 1_3:+58647125 Msa0128810:CDS
ACTTCTTCTGTTGGCCTCGC+TGG 0.403590 1_3:+58646073 Msa0128810:CDS
ACAGTGCATACGCTCTGAAA+TGG 0.408844 1_3:+58645978 Msa0128810:CDS
TCTGTTGGCCTCGCTGGTCC+TGG 0.423133 1_3:+58646079 Msa0128810:CDS
GATTATATATTTATTCTTGT+AGG 0.425496 1_3:-58647759 None:intergenic
ACGGTTATGGGGCTATAAAA+AGG 0.451906 1_3:+58647160 Msa0128810:CDS
TTGTGACTTAGTTTATCCTA+TGG 0.465564 1_3:+58647820 Msa0128810:three_prime_UTR
ACAAAGTAAAATAAGGCCTC+CGG 0.473405 1_3:-58647906 None:intergenic
CCCGCCGCTACTTCTTCTGT+TGG 0.478264 1_3:+58646064 Msa0128810:CDS
ATACTTTATGAAAATGGCAA+TGG 0.499210 1_3:+58648000 Msa0128810:three_prime_UTR
GCTATTTCACTTTAGCTGAA+AGG 0.508675 1_3:-58647781 None:intergenic
TCAGGTAAATGATGTAATAG+TGG 0.511125 1_3:+58647056 Msa0128810:intron
ATCTTGGTTGTAGGTAGTCA+CGG 0.516671 1_3:+58647141 Msa0128810:CDS
TGCCAATATAGATCAGTGCT+TGG 0.536544 1_3:-58647733 None:intergenic
CAAAGTAAAATAAGGCCTCC+GGG 0.542553 1_3:-58647905 None:intergenic
TGATGTAATAGTGGAGGTGG+TGG 0.556420 1_3:+58647065 Msa0128810:CDS
AAATAAGGCCTCCGGGTAGG+AGG 0.557118 1_3:-58647898 None:intergenic
AAATGATGTAATAGTGGAGG+TGG 0.562868 1_3:+58647062 Msa0128810:CDS
GATTGTTGTTGCCTCCTACC+CGG 0.564104 1_3:+58647887 Msa0128810:three_prime_UTR
GTAAAATAAGGCCTCCGGGT+AGG 0.586447 1_3:-58647901 None:intergenic
CTACAAACCAAACATGCCAT+AGG 0.591562 1_3:-58647836 None:intergenic
AGTGTACGCAGGAGCTGCTG+AGG 0.594737 1_3:+58646185 Msa0128810:intron
TGTAGGTAGTCACGGTTATG+GGG 0.599167 1_3:+58647149 Msa0128810:CDS
ATAGAAAATATGCAATTGTG+TGG 0.605193 1_3:+58647865 Msa0128810:three_prime_UTR
TTGTGCTAATTAGTGTACGC+AGG 0.608881 1_3:+58646174 Msa0128810:intron
GCCAACAGAAGAAGTAGCGG+CGG 0.613641 1_3:-58646065 None:intergenic
GAGGCCAACAGAAGAAGTAG+CGG 0.621681 1_3:-58646068 None:intergenic
CCAACAGAAGAAGTAGCGGC+GGG 0.624059 1_3:-58646064 None:intergenic
GTAATAGTGGAGGTGGTGGA+AGG 0.626493 1_3:+58647069 Msa0128810:CDS
GGTAAATGATGTAATAGTGG+AGG 0.637317 1_3:+58647059 Msa0128810:intron
TGTTGTTGCCTCCTACCCGG+AGG 0.694844 1_3:+58647890 Msa0128810:three_prime_UTR
GAATTATACCAGGACCAGCG+AGG 0.726201 1_3:-58646087 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATGAAAAGAATTA+GGG - chr1_3:58646481-58646500 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG - chr1_3:58646482-58646501 None:intergenic 10.0%
!!! TTTCATAATTTTATTTGATA+AGG + chr1_3:58646488-58646507 Msa0128810:intron 10.0%
!!! ATGATATTATTTTAGATAAT+AGG + chr1_3:58646614-58646633 Msa0128810:intron 10.0%
!! TAAAATTATGAAAAGAATTA+GGG - chr1_3:58646481-58646500 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG - chr1_3:58646482-58646501 None:intergenic 10.0%
!!! TTTCATAATTTTATTTGATA+AGG + chr1_3:58646488-58646507 Msa0128810:intron 10.0%
!!! ATGATATTATTTTAGATAAT+AGG + chr1_3:58646614-58646633 Msa0128810:intron 10.0%
!!! ATTTTATTTGATAAGGAAAA+TGG + chr1_3:58646495-58646514 Msa0128810:intron 15.0%
!! AAAAATGTTAGAATTAAATG+CGG - chr1_3:58647301-58647320 None:intergenic 15.0%
!!! ATTACATTTATTCATGTTTT+TGG - chr1_3:58647368-58647387 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG + chr1_3:58647540-58647559 Msa0128810:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG + chr1_3:58647541-58647560 Msa0128810:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG + chr1_3:58647542-58647561 Msa0128810:intron 15.0%
!! GATTATATATTTATTCTTGT+AGG - chr1_3:58647762-58647781 None:intergenic 15.0%
!!! TGAAAAATAATCATTTGTTA+AGG + chr1_3:58648044-58648063 Msa0128810:three_prime_UTR 15.0%
!!! ATTTTATTTGATAAGGAAAA+TGG + chr1_3:58646495-58646514 Msa0128810:intron 15.0%
!! AAAAATGTTAGAATTAAATG+CGG - chr1_3:58647301-58647320 None:intergenic 15.0%
!!! ATTACATTTATTCATGTTTT+TGG - chr1_3:58647368-58647387 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG + chr1_3:58647540-58647559 Msa0128810:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG + chr1_3:58647541-58647560 Msa0128810:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG + chr1_3:58647542-58647561 Msa0128810:intron 15.0%
!! GATTATATATTTATTCTTGT+AGG - chr1_3:58647762-58647781 None:intergenic 15.0%
!!! TTTGTTGATGTTTTGATAAT+TGG + chr1_3:58646142-58646161 Msa0128810:intron 20.0%
!! AACAAAAATAGAAACACATA+CGG - chr1_3:58646292-58646311 None:intergenic 20.0%
!!! AAAATTTTAAACAACAGTCA+AGG + chr1_3:58646328-58646347 Msa0128810:intron 20.0%
!!! TTTTTTGTTAGTTTGATGAA+AGG + chr1_3:58646726-58646745 Msa0128810:intron 20.0%
!!! TAATGTATACATTTTTCCTT+AGG - chr1_3:58646981-58647000 None:intergenic 20.0%
!! ACATAATTATAAAGTCTCTA+TGG + chr1_3:58647462-58647481 Msa0128810:intron 20.0%
!! AAATAAACTTAGAAAGCAAA+AGG - chr1_3:58647519-58647538 None:intergenic 20.0%
!!! TTTCATTTTATTTATACTGG+GGG + chr1_3:58647543-58647562 Msa0128810:intron 20.0%
!! TTCATGTACAAAGTAAAATA+AGG - chr1_3:58647916-58647935 None:intergenic 20.0%
!!! TTTGTTGATGTTTTGATAAT+TGG + chr1_3:58646142-58646161 Msa0128810:intron 20.0%
!! AACAAAAATAGAAACACATA+CGG - chr1_3:58646292-58646311 None:intergenic 20.0%
!!! AAAATTTTAAACAACAGTCA+AGG + chr1_3:58646328-58646347 Msa0128810:intron 20.0%
!!! TTTTTTGTTAGTTTGATGAA+AGG + chr1_3:58646726-58646745 Msa0128810:intron 20.0%
!!! TAATGTATACATTTTTCCTT+AGG - chr1_3:58646981-58647000 None:intergenic 20.0%
!! ACATAATTATAAAGTCTCTA+TGG + chr1_3:58647462-58647481 Msa0128810:intron 20.0%
!! AAATAAACTTAGAAAGCAAA+AGG - chr1_3:58647519-58647538 None:intergenic 20.0%
!!! TTTCATTTTATTTATACTGG+GGG + chr1_3:58647543-58647562 Msa0128810:intron 20.0%
!! TTCATGTACAAAGTAAAATA+AGG - chr1_3:58647916-58647935 None:intergenic 20.0%
! ATGAAATTGAGAATTATACC+AGG - chr1_3:58646100-58646119 None:intergenic 25.0%
!! TGAAATTTCTCTGGTTTATA+CGG + chr1_3:58646530-58646549 Msa0128810:intron 25.0%
! TTACCATCATCTTTGTATTT+CGG + chr1_3:58646839-58646858 Msa0128810:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG - chr1_3:58646926-58646945 None:intergenic 25.0%
! TTAAGTACTTATGTCATATG+TGG + chr1_3:58647007-58647026 Msa0128810:intron 25.0%
! ATAGAAAATATGCAATTGTG+TGG + chr1_3:58647865-58647884 Msa0128810:three_prime_UTR 25.0%
!! GCAAAAAACAGTAATTTTTC+AGG - chr1_3:58647976-58647995 None:intergenic 25.0%
! ATACTTTATGAAAATGGCAA+TGG + chr1_3:58648000-58648019 Msa0128810:three_prime_UTR 25.0%
! ATGAAATTGAGAATTATACC+AGG - chr1_3:58646100-58646119 None:intergenic 25.0%
!! TGAAATTTCTCTGGTTTATA+CGG + chr1_3:58646530-58646549 Msa0128810:intron 25.0%
! TTACCATCATCTTTGTATTT+CGG + chr1_3:58646839-58646858 Msa0128810:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG - chr1_3:58646926-58646945 None:intergenic 25.0%
! TTAAGTACTTATGTCATATG+TGG + chr1_3:58647007-58647026 Msa0128810:intron 25.0%
! ATAGAAAATATGCAATTGTG+TGG + chr1_3:58647865-58647884 Msa0128810:three_prime_UTR 25.0%
!! GCAAAAAACAGTAATTTTTC+AGG - chr1_3:58647976-58647995 None:intergenic 25.0%
! ATACTTTATGAAAATGGCAA+TGG + chr1_3:58648000-58648019 Msa0128810:three_prime_UTR 25.0%
!! TCCGTTAGTGATTTTTAGAA+TGG + chr1_3:58645883-58645902 Msa0128810:exon 30.0%
! GCCATTCTAAAAATCACTAA+CGG - chr1_3:58645887-58645906 None:intergenic 30.0%
TTCGGTTGTTACAAATGTTA+TGG + chr1_3:58646359-58646378 Msa0128810:intron 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG + chr1_3:58646506-58646525 Msa0128810:intron 30.0%
TTGAATGGATGAAATTTCTC+TGG + chr1_3:58646521-58646540 Msa0128810:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG + chr1_3:58646553-58646572 Msa0128810:intron 30.0%
CCTGTATTTATATGTTGTGT+GGG + chr1_3:58646554-58646573 Msa0128810:intron 30.0%
GATGAAAGGAGATAAATTGA+AGG + chr1_3:58646740-58646759 Msa0128810:intron 30.0%
TTCACTTAAATTGCTTCAAC+TGG + chr1_3:58646805-58646824 Msa0128810:intron 30.0%
TAACCGAAATACAAAGATGA+TGG - chr1_3:58646845-58646864 None:intergenic 30.0%
! TCTGATAACTGAATTTTCTC+AGG + chr1_3:58647038-58647057 Msa0128810:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG + chr1_3:58647056-58647075 Msa0128810:intron 30.0%
TACAATTATCATCCTTCGTT+TGG + chr1_3:58647200-58647219 Msa0128810:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG + chr1_3:58647259-58647278 Msa0128810:intron 30.0%
AATTAAATGCGGCATATTCA+TGG - chr1_3:58647290-58647309 None:intergenic 30.0%
AAACTTAGAAAGCAAAAGGA+AGG - chr1_3:58647515-58647534 None:intergenic 30.0%
TTGTGACTTAGTTTATCCTA+TGG + chr1_3:58647820-58647839 Msa0128810:three_prime_UTR 30.0%
GCCTTGATACTTTATGAAAA+TGG + chr1_3:58647994-58648013 Msa0128810:three_prime_UTR 30.0%
!! GCCATTTTCATAAAGTATCA+AGG - chr1_3:58647998-58648017 None:intergenic 30.0%
!! TCCGTTAGTGATTTTTAGAA+TGG + chr1_3:58645883-58645902 Msa0128810:exon 30.0%
! GCCATTCTAAAAATCACTAA+CGG - chr1_3:58645887-58645906 None:intergenic 30.0%
TTCGGTTGTTACAAATGTTA+TGG + chr1_3:58646359-58646378 Msa0128810:intron 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG + chr1_3:58646506-58646525 Msa0128810:intron 30.0%
TTGAATGGATGAAATTTCTC+TGG + chr1_3:58646521-58646540 Msa0128810:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG + chr1_3:58646553-58646572 Msa0128810:intron 30.0%
CCTGTATTTATATGTTGTGT+GGG + chr1_3:58646554-58646573 Msa0128810:intron 30.0%
GATGAAAGGAGATAAATTGA+AGG + chr1_3:58646740-58646759 Msa0128810:intron 30.0%
TTCACTTAAATTGCTTCAAC+TGG + chr1_3:58646805-58646824 Msa0128810:intron 30.0%
TAACCGAAATACAAAGATGA+TGG - chr1_3:58646845-58646864 None:intergenic 30.0%
! TCTGATAACTGAATTTTCTC+AGG + chr1_3:58647038-58647057 Msa0128810:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG + chr1_3:58647056-58647075 Msa0128810:intron 30.0%
TACAATTATCATCCTTCGTT+TGG + chr1_3:58647200-58647219 Msa0128810:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG + chr1_3:58647259-58647278 Msa0128810:intron 30.0%
AATTAAATGCGGCATATTCA+TGG - chr1_3:58647290-58647309 None:intergenic 30.0%
AAACTTAGAAAGCAAAAGGA+AGG - chr1_3:58647515-58647534 None:intergenic 30.0%
TTGTGACTTAGTTTATCCTA+TGG + chr1_3:58647820-58647839 Msa0128810:three_prime_UTR 30.0%
GCCTTGATACTTTATGAAAA+TGG + chr1_3:58647994-58648013 Msa0128810:three_prime_UTR 30.0%
!! GCCATTTTCATAAAGTATCA+AGG - chr1_3:58647998-58648017 None:intergenic 30.0%
TCGATTGCAACTAACATTAC+TGG - chr1_3:58645947-58645966 None:intergenic 35.0%
CTCAAATCAGAATCCACAAT+TGG - chr1_3:58646216-58646235 None:intergenic 35.0%
CCCACACAACATATAAATAC+AGG - chr1_3:58646557-58646576 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG + chr1_3:58646560-58646579 Msa0128810:intron 35.0%
! TCGGTTAATTGTCATGCTTT+TGG + chr1_3:58646858-58646877 Msa0128810:intron 35.0%
AACTAATAGGGATATGGGTA+TGG - chr1_3:58646899-58646918 None:intergenic 35.0%
TACCCATATCCCTATTAGTT+AGG + chr1_3:58646899-58646918 Msa0128810:intron 35.0%
ACCCATATCCCTATTAGTTA+GGG + chr1_3:58646900-58646919 Msa0128810:intron 35.0%
TTTGTTGGCCCTAACTAATA+GGG - chr1_3:58646911-58646930 None:intergenic 35.0%
! TTTTGTTGGCCCTAACTAAT+AGG - chr1_3:58646912-58646931 None:intergenic 35.0%
! TTTTTCTACTTTCGAGCCTA+AGG + chr1_3:58646962-58646981 Msa0128810:intron 35.0%
GGTAAATGATGTAATAGTGG+AGG + chr1_3:58647059-58647078 Msa0128810:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG + chr1_3:58647062-58647081 Msa0128810:CDS 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG + chr1_3:58647125-58647144 Msa0128810:CDS 35.0%
TATGGGGCTATAAAAAGGTA+TGG + chr1_3:58647165-58647184 Msa0128810:intron 35.0%
! AAAAAGGTATGGCTTCATTG+TGG + chr1_3:58647176-58647195 Msa0128810:intron 35.0%
TTACATATCACTCCAAACGA+AGG - chr1_3:58647215-58647234 None:intergenic 35.0%
TAATCTTGTGTTTGTGATCG+TGG - chr1_3:58647340-58647359 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG - chr1_3:58647394-58647413 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG + chr1_3:58647403-58647422 Msa0128810:intron 35.0%
CAAAAGGAAGGTTTGATTGT+TGG - chr1_3:58647503-58647522 None:intergenic 35.0%
!! ATAGATCAGTGCTTGGTTTT+TGG - chr1_3:58647729-58647748 None:intergenic 35.0%
!! AACCAAGCACTGATCTATAT+TGG + chr1_3:58647731-58647750 Msa0128810:exon 35.0%
! GCTATTTCACTTTAGCTGAA+AGG - chr1_3:58647784-58647803 None:intergenic 35.0%
AGTTTATCCTATGGCATGTT+TGG + chr1_3:58647829-58647848 Msa0128810:three_prime_UTR 35.0%
ACAAAGTAAAATAAGGCCTC+CGG - chr1_3:58647909-58647928 None:intergenic 35.0%
TCGATTGCAACTAACATTAC+TGG - chr1_3:58645947-58645966 None:intergenic 35.0%
CTCAAATCAGAATCCACAAT+TGG - chr1_3:58646216-58646235 None:intergenic 35.0%
CCCACACAACATATAAATAC+AGG - chr1_3:58646557-58646576 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG + chr1_3:58646560-58646579 Msa0128810:intron 35.0%
! TCGGTTAATTGTCATGCTTT+TGG + chr1_3:58646858-58646877 Msa0128810:intron 35.0%
AACTAATAGGGATATGGGTA+TGG - chr1_3:58646899-58646918 None:intergenic 35.0%
TACCCATATCCCTATTAGTT+AGG + chr1_3:58646899-58646918 Msa0128810:intron 35.0%
ACCCATATCCCTATTAGTTA+GGG + chr1_3:58646900-58646919 Msa0128810:intron 35.0%
TTTGTTGGCCCTAACTAATA+GGG - chr1_3:58646911-58646930 None:intergenic 35.0%
! TTTTGTTGGCCCTAACTAAT+AGG - chr1_3:58646912-58646931 None:intergenic 35.0%
! TTTTTCTACTTTCGAGCCTA+AGG + chr1_3:58646962-58646981 Msa0128810:intron 35.0%
GGTAAATGATGTAATAGTGG+AGG + chr1_3:58647059-58647078 Msa0128810:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG + chr1_3:58647062-58647081 Msa0128810:CDS 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG + chr1_3:58647125-58647144 Msa0128810:CDS 35.0%
TATGGGGCTATAAAAAGGTA+TGG + chr1_3:58647165-58647184 Msa0128810:intron 35.0%
! AAAAAGGTATGGCTTCATTG+TGG + chr1_3:58647176-58647195 Msa0128810:intron 35.0%
TTACATATCACTCCAAACGA+AGG - chr1_3:58647215-58647234 None:intergenic 35.0%
TAATCTTGTGTTTGTGATCG+TGG - chr1_3:58647340-58647359 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG - chr1_3:58647394-58647413 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG + chr1_3:58647403-58647422 Msa0128810:intron 35.0%
CAAAAGGAAGGTTTGATTGT+TGG - chr1_3:58647503-58647522 None:intergenic 35.0%
!! ATAGATCAGTGCTTGGTTTT+TGG - chr1_3:58647729-58647748 None:intergenic 35.0%
!! AACCAAGCACTGATCTATAT+TGG + chr1_3:58647731-58647750 Msa0128810:exon 35.0%
! GCTATTTCACTTTAGCTGAA+AGG - chr1_3:58647784-58647803 None:intergenic 35.0%
AGTTTATCCTATGGCATGTT+TGG + chr1_3:58647829-58647848 Msa0128810:three_prime_UTR 35.0%
ACAAAGTAAAATAAGGCCTC+CGG - chr1_3:58647909-58647928 None:intergenic 35.0%
TTGTGCTAATTAGTGTACGC+AGG + chr1_3:58646174-58646193 Msa0128810:intron 40.0%
GCCCTAACTAATAGGGATAT+GGG - chr1_3:58646904-58646923 None:intergenic 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG + chr1_3:58647141-58647160 Msa0128810:CDS 40.0%
TTGTAGGTAGTCACGGTTAT+GGG + chr1_3:58647148-58647167 Msa0128810:CDS 40.0%
ACGGTTATGGGGCTATAAAA+AGG + chr1_3:58647160-58647179 Msa0128810:CDS 40.0%
CTGCAGGTTAAAGATTTCAG+TGG - chr1_3:58647398-58647417 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG - chr1_3:58647620-58647639 None:intergenic 40.0%
! ACTCGACTCTTTTAATCTGC+AGG + chr1_3:58647634-58647653 Msa0128810:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG + chr1_3:58647635-58647654 Msa0128810:intron 40.0%
TGCCAATATAGATCAGTGCT+TGG - chr1_3:58647736-58647755 None:intergenic 40.0%
CTACAAACCAAACATGCCAT+AGG - chr1_3:58647839-58647858 None:intergenic 40.0%
CAAAGTAAAATAAGGCCTCC+GGG - chr1_3:58647908-58647927 None:intergenic 40.0%
TTGTGCTAATTAGTGTACGC+AGG + chr1_3:58646174-58646193 Msa0128810:intron 40.0%
GCCCTAACTAATAGGGATAT+GGG - chr1_3:58646904-58646923 None:intergenic 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG + chr1_3:58647141-58647160 Msa0128810:CDS 40.0%
TTGTAGGTAGTCACGGTTAT+GGG + chr1_3:58647148-58647167 Msa0128810:CDS 40.0%
ACGGTTATGGGGCTATAAAA+AGG + chr1_3:58647160-58647179 Msa0128810:CDS 40.0%
CTGCAGGTTAAAGATTTCAG+TGG - chr1_3:58647398-58647417 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG - chr1_3:58647620-58647639 None:intergenic 40.0%
! ACTCGACTCTTTTAATCTGC+AGG + chr1_3:58647634-58647653 Msa0128810:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG + chr1_3:58647635-58647654 Msa0128810:intron 40.0%
TGCCAATATAGATCAGTGCT+TGG - chr1_3:58647736-58647755 None:intergenic 40.0%
CTACAAACCAAACATGCCAT+AGG - chr1_3:58647839-58647858 None:intergenic 40.0%
CAAAGTAAAATAAGGCCTCC+GGG - chr1_3:58647908-58647927 None:intergenic 40.0%
!! ACAGTGCATACGCTCTGAAA+TGG + chr1_3:58645978-58645997 Msa0128810:CDS 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG + chr1_3:58646200-58646219 Msa0128810:CDS 45.0%
ACAGTCAAGGTGTGCGATTT+CGG + chr1_3:58646341-58646360 Msa0128810:intron 45.0%
GGGACTTGGAGCATACTATA+TGG + chr1_3:58646574-58646593 Msa0128810:intron 45.0%
TAGCTGAATGCATCATGCTC+AGG + chr1_3:58646651-58646670 Msa0128810:intron 45.0%
GGCCCTAACTAATAGGGATA+TGG - chr1_3:58646905-58646924 None:intergenic 45.0%
! TGATGTAATAGTGGAGGTGG+TGG + chr1_3:58647065-58647084 Msa0128810:CDS 45.0%
CGTGCTTCAATCTTGGTTGT+AGG + chr1_3:58647132-58647151 Msa0128810:CDS 45.0%
GTTGTAGGTAGTCACGGTTA+TGG + chr1_3:58647147-58647166 Msa0128810:CDS 45.0%
TGTAGGTAGTCACGGTTATG+GGG + chr1_3:58647149-58647168 Msa0128810:CDS 45.0%
TCTTGTGTTTGTGATCGTGG+TGG - chr1_3:58647337-58647356 None:intergenic 45.0%
! GACTCTTTTAATCTGCAGGG+CGG + chr1_3:58647638-58647657 Msa0128810:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG + chr1_3:58647645-58647664 Msa0128810:intron 45.0%
!! ACAGTGCATACGCTCTGAAA+TGG + chr1_3:58645978-58645997 Msa0128810:CDS 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG + chr1_3:58646200-58646219 Msa0128810:CDS 45.0%
ACAGTCAAGGTGTGCGATTT+CGG + chr1_3:58646341-58646360 Msa0128810:intron 45.0%
GGGACTTGGAGCATACTATA+TGG + chr1_3:58646574-58646593 Msa0128810:intron 45.0%
TAGCTGAATGCATCATGCTC+AGG + chr1_3:58646651-58646670 Msa0128810:intron 45.0%
GGCCCTAACTAATAGGGATA+TGG - chr1_3:58646905-58646924 None:intergenic 45.0%
! TGATGTAATAGTGGAGGTGG+TGG + chr1_3:58647065-58647084 Msa0128810:CDS 45.0%
CGTGCTTCAATCTTGGTTGT+AGG + chr1_3:58647132-58647151 Msa0128810:CDS 45.0%
GTTGTAGGTAGTCACGGTTA+TGG + chr1_3:58647147-58647166 Msa0128810:CDS 45.0%
TGTAGGTAGTCACGGTTATG+GGG + chr1_3:58647149-58647168 Msa0128810:CDS 45.0%
TCTTGTGTTTGTGATCGTGG+TGG - chr1_3:58647337-58647356 None:intergenic 45.0%
! GACTCTTTTAATCTGCAGGG+CGG + chr1_3:58647638-58647657 Msa0128810:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG + chr1_3:58647645-58647664 Msa0128810:intron 45.0%
GAGGCCAACAGAAGAAGTAG+CGG - chr1_3:58646071-58646090 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG - chr1_3:58646090-58646109 None:intergenic 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG + chr1_3:58647069-58647088 Msa0128810:CDS 50.0%
TACTGCTGCCTATCTTCTGC+AGG - chr1_3:58647414-58647433 None:intergenic 50.0%
! GATTGTTGTTGCCTCCTACC+CGG + chr1_3:58647887-58647906 Msa0128810:three_prime_UTR 50.0%
GTAAAATAAGGCCTCCGGGT+AGG - chr1_3:58647904-58647923 None:intergenic 50.0%
GAGGCCAACAGAAGAAGTAG+CGG - chr1_3:58646071-58646090 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG - chr1_3:58646090-58646109 None:intergenic 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG + chr1_3:58647069-58647088 Msa0128810:CDS 50.0%
TACTGCTGCCTATCTTCTGC+AGG - chr1_3:58647414-58647433 None:intergenic 50.0%
! GATTGTTGTTGCCTCCTACC+CGG + chr1_3:58647887-58647906 Msa0128810:three_prime_UTR 50.0%
GTAAAATAAGGCCTCCGGGT+AGG - chr1_3:58647904-58647923 None:intergenic 50.0%
CCAACAGAAGAAGTAGCGGC+GGG - chr1_3:58646067-58646086 None:intergenic 55.0%
GCCAACAGAAGAAGTAGCGG+CGG - chr1_3:58646068-58646087 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG + chr1_3:58646073-58646092 Msa0128810:CDS 55.0%
AAATAAGGCCTCCGGGTAGG+AGG - chr1_3:58647901-58647920 None:intergenic 55.0%
CCAACAGAAGAAGTAGCGGC+GGG - chr1_3:58646067-58646086 None:intergenic 55.0%
GCCAACAGAAGAAGTAGCGG+CGG - chr1_3:58646068-58646087 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG + chr1_3:58646073-58646092 Msa0128810:CDS 55.0%
AAATAAGGCCTCCGGGTAGG+AGG - chr1_3:58647901-58647920 None:intergenic 55.0%
CCCGCCGCTACTTCTTCTGT+TGG + chr1_3:58646064-58646083 Msa0128810:CDS 60.0%
AGTGTACGCAGGAGCTGCTG+AGG + chr1_3:58646185-58646204 Msa0128810:intron 60.0%
TGTTGTTGCCTCCTACCCGG+AGG + chr1_3:58647890-58647909 Msa0128810:three_prime_UTR 60.0%
CCCGCCGCTACTTCTTCTGT+TGG + chr1_3:58646064-58646083 Msa0128810:CDS 60.0%
AGTGTACGCAGGAGCTGCTG+AGG + chr1_3:58646185-58646204 Msa0128810:intron 60.0%
TGTTGTTGCCTCCTACCCGG+AGG + chr1_3:58647890-58647909 Msa0128810:three_prime_UTR 60.0%
! TCTGTTGGCCTCGCTGGTCC+TGG + chr1_3:58646079-58646098 Msa0128810:CDS 65.0%
! TCTGTTGGCCTCGCTGGTCC+TGG + chr1_3:58646079-58646098 Msa0128810:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58645840 58648064 58645840 ID=Msa0128810;Name=Msa0128810
chr1_3 mRNA 58645840 58648064 58645840 ID=Msa0128810-mRNA-1;Parent=Msa0128810;Name=Msa0128810-mRNA-1;_AED=0.04;_eAED=0.04;_QI=62|1|1|1|0.66|0.75|4|321|170
chr1_3 exon 58645840 58646100 58645840 ID=Msa0128810-mRNA-1:exon:18844;Parent=Msa0128810-mRNA-1
chr1_3 exon 58646187 58646290 58646187 ID=Msa0128810-mRNA-1:exon:18845;Parent=Msa0128810-mRNA-1
chr1_3 exon 58647060 58647181 58647060 ID=Msa0128810-mRNA-1:exon:18846;Parent=Msa0128810-mRNA-1
chr1_3 exon 58647656 58648064 58647656 ID=Msa0128810-mRNA-1:exon:18847;Parent=Msa0128810-mRNA-1
chr1_3 five_prime_UTR 58645840 58645901 58645840 ID=Msa0128810-mRNA-1:five_prime_utr;Parent=Msa0128810-mRNA-1
chr1_3 CDS 58645902 58646100 58645902 ID=Msa0128810-mRNA-1:cds;Parent=Msa0128810-mRNA-1
chr1_3 CDS 58646187 58646290 58646187 ID=Msa0128810-mRNA-1:cds;Parent=Msa0128810-mRNA-1
chr1_3 CDS 58647060 58647181 58647060 ID=Msa0128810-mRNA-1:cds;Parent=Msa0128810-mRNA-1
chr1_3 CDS 58647656 58647743 58647656 ID=Msa0128810-mRNA-1:cds;Parent=Msa0128810-mRNA-1
chr1_3 three_prime_UTR 58647744 58648064 58647744 ID=Msa0128810-mRNA-1:three_prime_utr;Parent=Msa0128810-mRNA-1
Gene Sequence

>Msa0128810

ATGGCGAGTGCGAATTCAGAACAATCAACAGAGAAATCACAACCAGTAATGTTAGTTGCAATCGATGAGAGCGATCACAGTGCATACGCTCTGAAATGGACTCTCGATCATTTCTTCAGCACAAACAATCATGTTTTCAAACTTGTTCTTGTTCATGCTAGACCCGCCGCTACTTCTTCTGTTGGCCTCGCTGGTCCTGTGTACGCAGGAGCTGCTGAGGTTTTGCCAATTGTGGATTCTGATTTGAGAAAGATTGCTGCTAGAGTTGCTGAGAATGCTAAACAACTTTGCATTAAGAAATCCGTAAATGATGTAATAGTGGAGGTGGTGGAAGGTGATGCTAGAAATGTTCTCTGTGATACAGTAGAAAAGTATCGTGCTTCAATCTTGGTTGTAGGTAGTCACGGTTATGGGGCTATAAAAAGGGCGGTTTTAGGTAGTGTGAGTGACTACTGTGCTCATCATGCTCATTGCACTGTGATGATTGTGAAGAAGCCAAAAACCAAGCACTGA
Protein sequence

>Msa0128810

MASANSEQSTEKSQPVMLVAIDESDHSAYALKWTLDHFFSTNNHVFKLVLVHARPAATSSVGLAGPVYAGAAEVLPIVDSDLRKIAARVAENAKQLCIKKSVNDVIVEVVEGDARNVLCDTVEKYRASILVVGSHGYGAIKRAVLGSVSDYCAHHAHCTVMIVKKPKTKH*