AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0082240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082240 MtrunA17_Chr1g0195661 94.118 170 4 2 1 167 1 167 1.16e-112 317
Msa0082240 MtrunA17_Chr7g0269301 68.831 154 46 2 16 167 9 162 4.92e-66 199
Msa0082240 MtrunA17_Chr1g0176321 40.000 170 81 4 18 167 6 174 1.95e-33 116
Msa0082240 MtrunA17_Chr1g0176311 40.244 164 83 4 18 167 25 187 4.61e-33 115
Msa0082240 MtrunA17_Chr1g0194801 40.764 157 89 3 11 163 2 158 6.05e-33 114
Msa0082240 MtrunA17_Chr1g0192651 39.752 161 86 5 10 165 5 159 2.06e-31 110
Msa0082240 MtrunA17_Chr5g0427291 33.951 162 91 4 16 161 9 170 7.69e-23 89.4
Msa0082240 MtrunA17_Chr2g0323291 30.818 159 94 3 19 164 29 184 3.55e-18 78.2
Msa0082240 MtrunA17_Chr4g0032261 34.211 152 90 4 19 162 9 158 2.12e-17 74.7
Msa0082240 MtrunA17_Chr4g0001641 49.206 63 32 0 99 161 3 65 9.88e-16 68.2
Msa0082240 MtrunA17_Chr4g0008801 31.373 153 91 4 19 161 9 157 1.38e-13 64.7
Msa0082240 MtrunA17_Chr5g0433951 29.221 154 93 5 19 161 9 157 1.85e-13 64.7
Msa0082240 MtrunA17_Chr2g0294421 28.025 157 103 4 19 167 9 163 8.56e-13 62.8
Msa0082240 MtrunA17_Chr7g0240701 30.968 155 89 6 19 161 9 157 1.88e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082240 AT2G47710.1 63.354 161 57 1 8 166 1 161 4.91e-73 217
Msa0082240 AT3G11930.1 40.678 177 85 5 7 164 20 195 8.28e-35 121
Msa0082240 AT3G11930.2 39.888 178 86 5 7 164 20 196 2.53e-34 119
Msa0082240 AT3G11930.4 39.665 179 86 5 7 164 20 197 2.72e-34 119
Msa0082240 AT1G09740.2 38.994 159 84 5 17 164 10 166 8.38e-29 104
Msa0082240 AT1G09740.1 38.994 159 84 5 17 164 10 166 8.38e-29 104
Msa0082240 AT3G11930.3 34.783 207 82 7 7 164 20 222 2.74e-28 105
Msa0082240 AT5G49050.1 44.737 114 61 1 13 124 37 150 3.30e-27 100
Msa0082240 AT3G58450.1 37.888 161 86 4 17 164 32 191 1.44e-26 100
Msa0082240 AT3G58450.2 36.774 155 89 3 17 164 32 184 7.79e-26 98.2
Msa0082240 AT3G62550.1 36.527 167 84 7 7 162 2 157 5.91e-25 94.7
Msa0082240 AT1G11360.2 33.721 172 93 5 10 166 33 198 1.51e-19 82.8
Msa0082240 AT1G11360.1 33.721 172 93 5 10 166 33 198 1.51e-19 82.8
Msa0082240 AT1G11360.4 33.721 172 93 5 10 166 33 198 1.51e-19 82.8
Msa0082240 AT1G11360.3 33.721 172 93 5 10 166 33 198 1.51e-19 82.8
Msa0082240 AT1G68300.1 35.220 159 91 7 11 161 3 157 1.74e-17 75.5
Msa0082240 AT5G14680.1 28.144 167 99 6 12 162 2 163 6.21e-15 68.9
Msa0082240 AT3G17020.1 28.947 152 97 4 19 161 11 160 1.57e-13 65.1
Msa0082240 AT3G01520.1 29.078 141 85 3 30 162 30 163 1.98e-13 65.1
Msa0082240 AT3G53990.1 26.797 153 98 4 19 161 9 157 5.26e-13 63.5
Msa0082240 AT2G21620.2 30.201 149 80 4 17 160 42 171 1.41e-11 60.5
Msa0082240 AT2G21620.1 29.861 144 81 3 17 160 42 165 1.50e-11 60.1
Msa0082240 AT3G03270.2 28.750 160 86 7 19 161 9 157 8.78e-11 57.8

Find 40 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTGATACTTTATGAAAA+TGG 0.199567 1_2:+61036584 Msa0082240:three_prime_UTR
TTCATATACAAAGTAAAATA+AGG 0.268743 1_2:-61036503 None:intergenic
TTGTAGGTAGTCACGGTTAT+GGG 0.301582 1_2:+61035742 Msa0082240:CDS
AGTTTATCCTATGGCATGTT+TGG 0.308645 1_2:+61036419 Msa0082240:three_prime_UTR
TCGATTGCAACTAACATTAC+TGG 0.334213 1_2:-61034547 None:intergenic
GTTGTAGGTAGTCACGGTTA+TGG 0.361010 1_2:+61035741 Msa0082240:CDS
CGTGCTTCAATCTTGGTTGT+AGG 0.387207 1_2:+61035726 Msa0082240:CDS
CTCAAATCAGAATCCACAAT+TGG 0.390759 1_2:-61034815 None:intergenic
AAAGTATCGTGCTTCAATCT+TGG 0.402940 1_2:+61035719 Msa0082240:CDS
ACTTCTTCTGTTGGCCTCGC+TGG 0.403590 1_2:+61034676 Msa0082240:CDS
ACAGTGCATACGCTCTGAAA+TGG 0.408844 1_2:+61034581 Msa0082240:CDS
TCTGTTGGCCTCGCTGGTCC+TGG 0.423133 1_2:+61034682 Msa0082240:CDS
GATTATATATTTATTCTTGT+AGG 0.425496 1_2:-61036349 None:intergenic
AACCAAGCACTGATCTATAC+TGG 0.427898 1_2:+61036321 Msa0082240:exon
ACGGTTATGGGGCTATAAAA+AGG 0.451906 1_2:+61035754 Msa0082240:CDS
ACAAAGTAAAATAAGGCCTC+CGG 0.457393 1_2:-61036496 None:intergenic
TTGTGACTTAGTTTATCCTA+TGG 0.465564 1_2:+61036410 Msa0082240:three_prime_UTR
ATACTTTATGAAAATGGCAA+TGG 0.499210 1_2:+61036590 Msa0082240:three_prime_UTR
GCTATTTCACTTTAGCTGAA+AGG 0.508675 1_2:-61036371 None:intergenic
TCAGGTAAATGATGTAATAG+TGG 0.511125 1_2:+61035650 Msa0082240:intron
ATCTTGGTTGTAGGTAGTCA+CGG 0.516671 1_2:+61035735 Msa0082240:CDS
CCCACCGCTACTTCTTCTGT+TGG 0.524480 1_2:+61034667 Msa0082240:CDS
CAAAGTAAAATAAGGCCTCC+GGG 0.542553 1_2:-61036495 None:intergenic
TGCCAGTATAGATCAGTGCT+TGG 0.553700 1_2:-61036323 None:intergenic
TGATGTAATAGTGGAGGTGG+TGG 0.556420 1_2:+61035659 Msa0082240:CDS
AAATAAGGCCTCCGGGTAGG+AGG 0.557118 1_2:-61036488 None:intergenic
AAATGATGTAATAGTGGAGG+TGG 0.562868 1_2:+61035656 Msa0082240:CDS
GATTGTTGTTGCCTCCTACC+CGG 0.564104 1_2:+61036477 Msa0082240:three_prime_UTR
GTAAAATAAGGCCTCCGGGT+AGG 0.586447 1_2:-61036491 None:intergenic
CTACAAACCAAACATGCCAT+AGG 0.591562 1_2:-61036426 None:intergenic
AGTGTACGCAGGAGCTGCTG+AGG 0.594737 1_2:+61034787 Msa0082240:intron
TGTAGGTAGTCACGGTTATG+GGG 0.599167 1_2:+61035743 Msa0082240:CDS
ATAGAAAATATGCAATTGTG+TGG 0.605193 1_2:+61036455 Msa0082240:three_prime_UTR
GCCAACAGAAGAAGTAGCGG+TGG 0.605329 1_2:-61034668 None:intergenic
GTAATAGTGGAGGTGGTGGA+AGG 0.626493 1_2:+61035663 Msa0082240:CDS
GGTAAATGATGTAATAGTGG+AGG 0.637317 1_2:+61035653 Msa0082240:intron
GAGGCCAACAGAAGAAGTAG+CGG 0.655250 1_2:-61034671 None:intergenic
CCAACAGAAGAAGTAGCGGT+GGG 0.684778 1_2:-61034667 None:intergenic
TGTTGTTGCCTCCTACCCGG+AGG 0.694844 1_2:+61036480 Msa0082240:three_prime_UTR
GAATTATACCAGGACCAGCG+AGG 0.726201 1_2:-61034690 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATGAAAAGAATTA+GGG - chr1_2:61035077-61035096 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG - chr1_2:61035078-61035097 None:intergenic 10.0%
!!! TTTCATAATTTTATTTGATA+AGG + chr1_2:61035084-61035103 Msa0082240:intron 10.0%
!!! ATGATATTATTTTAGATAAT+AGG + chr1_2:61035210-61035229 Msa0082240:intron 10.0%
!! TAAAATTATGAAAAGAATTA+GGG - chr1_2:61035077-61035096 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG - chr1_2:61035078-61035097 None:intergenic 10.0%
!!! TTTCATAATTTTATTTGATA+AGG + chr1_2:61035084-61035103 Msa0082240:intron 10.0%
!!! ATGATATTATTTTAGATAAT+AGG + chr1_2:61035210-61035229 Msa0082240:intron 10.0%
!! AAAACAAAAATAGAAACATA+CGG - chr1_2:61034894-61034913 None:intergenic 15.0%
!!! ATTTTATTTGATAAGGAAAA+TGG + chr1_2:61035091-61035110 Msa0082240:intron 15.0%
!! AAAAATGTTAGAATTAAATG+CGG - chr1_2:61035892-61035911 None:intergenic 15.0%
!!! ATTACATTTATTCATGTTTT+TGG - chr1_2:61035959-61035978 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG + chr1_2:61036130-61036149 Msa0082240:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG + chr1_2:61036131-61036150 Msa0082240:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG + chr1_2:61036132-61036151 Msa0082240:intron 15.0%
!! GATTATATATTTATTCTTGT+AGG - chr1_2:61036352-61036371 None:intergenic 15.0%
!! TTCATATACAAAGTAAAATA+AGG - chr1_2:61036506-61036525 None:intergenic 15.0%
!! TTGAAAAATAATCATTGTTA+AGG + chr1_2:61036632-61036651 Msa0082240:three_prime_UTR 15.0%
!! AAAACAAAAATAGAAACATA+CGG - chr1_2:61034894-61034913 None:intergenic 15.0%
!!! ATTTTATTTGATAAGGAAAA+TGG + chr1_2:61035091-61035110 Msa0082240:intron 15.0%
!! AAAAATGTTAGAATTAAATG+CGG - chr1_2:61035892-61035911 None:intergenic 15.0%
!!! ATTACATTTATTCATGTTTT+TGG - chr1_2:61035959-61035978 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG + chr1_2:61036130-61036149 Msa0082240:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG + chr1_2:61036131-61036150 Msa0082240:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG + chr1_2:61036132-61036151 Msa0082240:intron 15.0%
!! GATTATATATTTATTCTTGT+AGG - chr1_2:61036352-61036371 None:intergenic 15.0%
!! TTCATATACAAAGTAAAATA+AGG - chr1_2:61036506-61036525 None:intergenic 15.0%
!!! TTTGTTGATGTTTTGATAAT+TGG + chr1_2:61034744-61034763 Msa0082240:intron 20.0%
!!! AAAATTTTAAACAACAGTCA+AGG + chr1_2:61034927-61034946 Msa0082240:intron 20.0%
!!! TTTTTTGTTAGTTTGATGAA+AGG + chr1_2:61035321-61035340 Msa0082240:intron 20.0%
!!! TAATGTATACATTTTTCCTT+AGG - chr1_2:61035575-61035594 None:intergenic 20.0%
!!! TTTCATTTTATTTATACTGG+GGG + chr1_2:61036133-61036152 Msa0082240:intron 20.0%
!!! TTTGTTGATGTTTTGATAAT+TGG + chr1_2:61034744-61034763 Msa0082240:intron 20.0%
!!! AAAATTTTAAACAACAGTCA+AGG + chr1_2:61034927-61034946 Msa0082240:intron 20.0%
!!! TTTTTTGTTAGTTTGATGAA+AGG + chr1_2:61035321-61035340 Msa0082240:intron 20.0%
!!! TAATGTATACATTTTTCCTT+AGG - chr1_2:61035575-61035594 None:intergenic 20.0%
!!! TTTCATTTTATTTATACTGG+GGG + chr1_2:61036133-61036152 Msa0082240:intron 20.0%
! ATGAAATTGAGAATTATACC+AGG - chr1_2:61034703-61034722 None:intergenic 25.0%
!!! TGAATTTTCTCTGGTTTATA+CGG + chr1_2:61035126-61035145 Msa0082240:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG - chr1_2:61035520-61035539 None:intergenic 25.0%
! TTAAGTACTTATGTCATATG+TGG + chr1_2:61035601-61035620 Msa0082240:intron 25.0%
! CAAATAACTTAGAAAGCAAA+AGG - chr1_2:61036110-61036129 None:intergenic 25.0%
! ATAGAAAATATGCAATTGTG+TGG + chr1_2:61036455-61036474 Msa0082240:three_prime_UTR 25.0%
!! GCAAAAAACAGTAATTTTTC+AGG - chr1_2:61036566-61036585 None:intergenic 25.0%
! ATACTTTATGAAAATGGCAA+TGG + chr1_2:61036590-61036609 Msa0082240:three_prime_UTR 25.0%
! ATGAAATTGAGAATTATACC+AGG - chr1_2:61034703-61034722 None:intergenic 25.0%
!!! TGAATTTTCTCTGGTTTATA+CGG + chr1_2:61035126-61035145 Msa0082240:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG - chr1_2:61035520-61035539 None:intergenic 25.0%
! TTAAGTACTTATGTCATATG+TGG + chr1_2:61035601-61035620 Msa0082240:intron 25.0%
! CAAATAACTTAGAAAGCAAA+AGG - chr1_2:61036110-61036129 None:intergenic 25.0%
! ATAGAAAATATGCAATTGTG+TGG + chr1_2:61036455-61036474 Msa0082240:three_prime_UTR 25.0%
!! GCAAAAAACAGTAATTTTTC+AGG - chr1_2:61036566-61036585 None:intergenic 25.0%
! ATACTTTATGAAAATGGCAA+TGG + chr1_2:61036590-61036609 Msa0082240:three_prime_UTR 25.0%
!! TCCGTTAGTGATTTTTAGAA+TGG + chr1_2:61034486-61034505 Msa0082240:exon 30.0%
! GCCATTCTAAAAATCACTAA+CGG - chr1_2:61034490-61034509 None:intergenic 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG + chr1_2:61035102-61035121 Msa0082240:intron 30.0%
! TTGAATGGATGAATTTTCTC+TGG + chr1_2:61035117-61035136 Msa0082240:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG + chr1_2:61035149-61035168 Msa0082240:intron 30.0%
CCTGTATTTATATGTTGTGT+GGG + chr1_2:61035150-61035169 Msa0082240:intron 30.0%
TGATGAAAGGAGATAATTGA+AGG + chr1_2:61035334-61035353 Msa0082240:intron 30.0%
TTCACTTAAATTGCTTCAAC+TGG + chr1_2:61035399-61035418 Msa0082240:intron 30.0%
TTACCATCATCTTTGTATGT+CGG + chr1_2:61035433-61035452 Msa0082240:intron 30.0%
! TCTGATAACTGAATTTTCTC+AGG + chr1_2:61035632-61035651 Msa0082240:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG + chr1_2:61035650-61035669 Msa0082240:intron 30.0%
GGTTACAAATTATCATCGTT+TGG + chr1_2:61035791-61035810 Msa0082240:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG + chr1_2:61035850-61035869 Msa0082240:intron 30.0%
AATTAAATGCGGCATATTCA+TGG - chr1_2:61035881-61035900 None:intergenic 30.0%
TAACTTAGAAAGCAAAAGGA+AGG - chr1_2:61036106-61036125 None:intergenic 30.0%
TTGTGACTTAGTTTATCCTA+TGG + chr1_2:61036410-61036429 Msa0082240:three_prime_UTR 30.0%
GCCTTGATACTTTATGAAAA+TGG + chr1_2:61036584-61036603 Msa0082240:three_prime_UTR 30.0%
!! GCCATTTTCATAAAGTATCA+AGG - chr1_2:61036588-61036607 None:intergenic 30.0%
!! TCCGTTAGTGATTTTTAGAA+TGG + chr1_2:61034486-61034505 Msa0082240:exon 30.0%
! GCCATTCTAAAAATCACTAA+CGG - chr1_2:61034490-61034509 None:intergenic 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG + chr1_2:61035102-61035121 Msa0082240:intron 30.0%
! TTGAATGGATGAATTTTCTC+TGG + chr1_2:61035117-61035136 Msa0082240:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG + chr1_2:61035149-61035168 Msa0082240:intron 30.0%
CCTGTATTTATATGTTGTGT+GGG + chr1_2:61035150-61035169 Msa0082240:intron 30.0%
TGATGAAAGGAGATAATTGA+AGG + chr1_2:61035334-61035353 Msa0082240:intron 30.0%
TTCACTTAAATTGCTTCAAC+TGG + chr1_2:61035399-61035418 Msa0082240:intron 30.0%
TTACCATCATCTTTGTATGT+CGG + chr1_2:61035433-61035452 Msa0082240:intron 30.0%
! TCTGATAACTGAATTTTCTC+AGG + chr1_2:61035632-61035651 Msa0082240:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG + chr1_2:61035650-61035669 Msa0082240:intron 30.0%
GGTTACAAATTATCATCGTT+TGG + chr1_2:61035791-61035810 Msa0082240:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG + chr1_2:61035850-61035869 Msa0082240:intron 30.0%
AATTAAATGCGGCATATTCA+TGG - chr1_2:61035881-61035900 None:intergenic 30.0%
TAACTTAGAAAGCAAAAGGA+AGG - chr1_2:61036106-61036125 None:intergenic 30.0%
TTGTGACTTAGTTTATCCTA+TGG + chr1_2:61036410-61036429 Msa0082240:three_prime_UTR 30.0%
GCCTTGATACTTTATGAAAA+TGG + chr1_2:61036584-61036603 Msa0082240:three_prime_UTR 30.0%
!! GCCATTTTCATAAAGTATCA+AGG - chr1_2:61036588-61036607 None:intergenic 30.0%
TCGATTGCAACTAACATTAC+TGG - chr1_2:61034550-61034569 None:intergenic 35.0%
CTCAAATCAGAATCCACAAT+TGG - chr1_2:61034818-61034837 None:intergenic 35.0%
TTCGGTTGTTACAGATGTTA+TGG + chr1_2:61034958-61034977 Msa0082240:intron 35.0%
CCCACACAACATATAAATAC+AGG - chr1_2:61035153-61035172 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG + chr1_2:61035156-61035175 Msa0082240:intron 35.0%
TAACCGACATACAAAGATGA+TGG - chr1_2:61035439-61035458 None:intergenic 35.0%
! TCGGTTAATTGTCATGCTTT+TGG + chr1_2:61035452-61035471 Msa0082240:intron 35.0%
AACTAATAGGGATATGGGTA+TGG - chr1_2:61035493-61035512 None:intergenic 35.0%
TACCCATATCCCTATTAGTT+AGG + chr1_2:61035493-61035512 Msa0082240:intron 35.0%
ACCCATATCCCTATTAGTTA+GGG + chr1_2:61035494-61035513 Msa0082240:intron 35.0%
TTTGTTGGCCCTAACTAATA+GGG - chr1_2:61035505-61035524 None:intergenic 35.0%
! TTTTGTTGGCCCTAACTAAT+AGG - chr1_2:61035506-61035525 None:intergenic 35.0%
! TTTTTCTACTTTCGAGCCTA+AGG + chr1_2:61035556-61035575 Msa0082240:intron 35.0%
GGTAAATGATGTAATAGTGG+AGG + chr1_2:61035653-61035672 Msa0082240:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG + chr1_2:61035656-61035675 Msa0082240:CDS 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG + chr1_2:61035719-61035738 Msa0082240:CDS 35.0%
TATGGGGCTATAAAAAGGTA+TGG + chr1_2:61035759-61035778 Msa0082240:intron 35.0%
! AAAAAGGTATGGCTTCATTG+TGG + chr1_2:61035770-61035789 Msa0082240:intron 35.0%
TAATCTTGTGTTTGTGATCG+TGG - chr1_2:61035931-61035950 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG - chr1_2:61035985-61036004 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG + chr1_2:61035994-61036013 Msa0082240:intron 35.0%
CAAAAGGAAGGTTTGATTGA+TGG - chr1_2:61036094-61036113 None:intergenic 35.0%
!! ATAGATCAGTGCTTGGTTTT+TGG - chr1_2:61036319-61036338 None:intergenic 35.0%
! GCTATTTCACTTTAGCTGAA+AGG - chr1_2:61036374-61036393 None:intergenic 35.0%
AGTTTATCCTATGGCATGTT+TGG + chr1_2:61036419-61036438 Msa0082240:three_prime_UTR 35.0%
ACAAAGTAAAATAAGGCCTC+CGG - chr1_2:61036499-61036518 None:intergenic 35.0%
TCGATTGCAACTAACATTAC+TGG - chr1_2:61034550-61034569 None:intergenic 35.0%
CTCAAATCAGAATCCACAAT+TGG - chr1_2:61034818-61034837 None:intergenic 35.0%
TTCGGTTGTTACAGATGTTA+TGG + chr1_2:61034958-61034977 Msa0082240:intron 35.0%
CCCACACAACATATAAATAC+AGG - chr1_2:61035153-61035172 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG + chr1_2:61035156-61035175 Msa0082240:intron 35.0%
TAACCGACATACAAAGATGA+TGG - chr1_2:61035439-61035458 None:intergenic 35.0%
! TCGGTTAATTGTCATGCTTT+TGG + chr1_2:61035452-61035471 Msa0082240:intron 35.0%
AACTAATAGGGATATGGGTA+TGG - chr1_2:61035493-61035512 None:intergenic 35.0%
TACCCATATCCCTATTAGTT+AGG + chr1_2:61035493-61035512 Msa0082240:intron 35.0%
ACCCATATCCCTATTAGTTA+GGG + chr1_2:61035494-61035513 Msa0082240:intron 35.0%
TTTGTTGGCCCTAACTAATA+GGG - chr1_2:61035505-61035524 None:intergenic 35.0%
! TTTTGTTGGCCCTAACTAAT+AGG - chr1_2:61035506-61035525 None:intergenic 35.0%
! TTTTTCTACTTTCGAGCCTA+AGG + chr1_2:61035556-61035575 Msa0082240:intron 35.0%
GGTAAATGATGTAATAGTGG+AGG + chr1_2:61035653-61035672 Msa0082240:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG + chr1_2:61035656-61035675 Msa0082240:CDS 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG + chr1_2:61035719-61035738 Msa0082240:CDS 35.0%
TATGGGGCTATAAAAAGGTA+TGG + chr1_2:61035759-61035778 Msa0082240:intron 35.0%
! AAAAAGGTATGGCTTCATTG+TGG + chr1_2:61035770-61035789 Msa0082240:intron 35.0%
TAATCTTGTGTTTGTGATCG+TGG - chr1_2:61035931-61035950 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG - chr1_2:61035985-61036004 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG + chr1_2:61035994-61036013 Msa0082240:intron 35.0%
CAAAAGGAAGGTTTGATTGA+TGG - chr1_2:61036094-61036113 None:intergenic 35.0%
!! ATAGATCAGTGCTTGGTTTT+TGG - chr1_2:61036319-61036338 None:intergenic 35.0%
! GCTATTTCACTTTAGCTGAA+AGG - chr1_2:61036374-61036393 None:intergenic 35.0%
AGTTTATCCTATGGCATGTT+TGG + chr1_2:61036419-61036438 Msa0082240:three_prime_UTR 35.0%
ACAAAGTAAAATAAGGCCTC+CGG - chr1_2:61036499-61036518 None:intergenic 35.0%
TTGTGCTAATTAGTGTACGC+AGG + chr1_2:61034776-61034795 Msa0082240:intron 40.0%
GCCCTAACTAATAGGGATAT+GGG - chr1_2:61035498-61035517 None:intergenic 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG + chr1_2:61035735-61035754 Msa0082240:CDS 40.0%
TTGTAGGTAGTCACGGTTAT+GGG + chr1_2:61035742-61035761 Msa0082240:CDS 40.0%
ACGGTTATGGGGCTATAAAA+AGG + chr1_2:61035754-61035773 Msa0082240:CDS 40.0%
CTGCAGGTTAAAGATTTCAG+TGG - chr1_2:61035989-61036008 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG - chr1_2:61036210-61036229 None:intergenic 40.0%
! ACTCGACTCTTTTAATCTGC+AGG + chr1_2:61036224-61036243 Msa0082240:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG + chr1_2:61036225-61036244 Msa0082240:intron 40.0%
!! AACCAAGCACTGATCTATAC+TGG + chr1_2:61036321-61036340 Msa0082240:exon 40.0%
CTACAAACCAAACATGCCAT+AGG - chr1_2:61036429-61036448 None:intergenic 40.0%
CAAAGTAAAATAAGGCCTCC+GGG - chr1_2:61036498-61036517 None:intergenic 40.0%
TTGTGCTAATTAGTGTACGC+AGG + chr1_2:61034776-61034795 Msa0082240:intron 40.0%
GCCCTAACTAATAGGGATAT+GGG - chr1_2:61035498-61035517 None:intergenic 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG + chr1_2:61035735-61035754 Msa0082240:CDS 40.0%
TTGTAGGTAGTCACGGTTAT+GGG + chr1_2:61035742-61035761 Msa0082240:CDS 40.0%
ACGGTTATGGGGCTATAAAA+AGG + chr1_2:61035754-61035773 Msa0082240:CDS 40.0%
CTGCAGGTTAAAGATTTCAG+TGG - chr1_2:61035989-61036008 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG - chr1_2:61036210-61036229 None:intergenic 40.0%
! ACTCGACTCTTTTAATCTGC+AGG + chr1_2:61036224-61036243 Msa0082240:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG + chr1_2:61036225-61036244 Msa0082240:intron 40.0%
!! AACCAAGCACTGATCTATAC+TGG + chr1_2:61036321-61036340 Msa0082240:exon 40.0%
CTACAAACCAAACATGCCAT+AGG - chr1_2:61036429-61036448 None:intergenic 40.0%
CAAAGTAAAATAAGGCCTCC+GGG - chr1_2:61036498-61036517 None:intergenic 40.0%
!! ACAGTGCATACGCTCTGAAA+TGG + chr1_2:61034581-61034600 Msa0082240:CDS 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG + chr1_2:61034802-61034821 Msa0082240:CDS 45.0%
ACAGTCAAGGTGTGCGATTT+CGG + chr1_2:61034940-61034959 Msa0082240:intron 45.0%
GGGACTTGGAGCATACTATA+TGG + chr1_2:61035170-61035189 Msa0082240:intron 45.0%
GGCCCTAACTAATAGGGATA+TGG - chr1_2:61035499-61035518 None:intergenic 45.0%
! TGATGTAATAGTGGAGGTGG+TGG + chr1_2:61035659-61035678 Msa0082240:CDS 45.0%
CGTGCTTCAATCTTGGTTGT+AGG + chr1_2:61035726-61035745 Msa0082240:CDS 45.0%
GTTGTAGGTAGTCACGGTTA+TGG + chr1_2:61035741-61035760 Msa0082240:CDS 45.0%
TGTAGGTAGTCACGGTTATG+GGG + chr1_2:61035743-61035762 Msa0082240:CDS 45.0%
TCTTGTGTTTGTGATCGTGG+TGG - chr1_2:61035928-61035947 None:intergenic 45.0%
! GACTCTTTTAATCTGCAGGG+CGG + chr1_2:61036228-61036247 Msa0082240:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG + chr1_2:61036235-61036254 Msa0082240:intron 45.0%
TGCCAGTATAGATCAGTGCT+TGG - chr1_2:61036326-61036345 None:intergenic 45.0%
!! ACAGTGCATACGCTCTGAAA+TGG + chr1_2:61034581-61034600 Msa0082240:CDS 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG + chr1_2:61034802-61034821 Msa0082240:CDS 45.0%
ACAGTCAAGGTGTGCGATTT+CGG + chr1_2:61034940-61034959 Msa0082240:intron 45.0%
GGGACTTGGAGCATACTATA+TGG + chr1_2:61035170-61035189 Msa0082240:intron 45.0%
GGCCCTAACTAATAGGGATA+TGG - chr1_2:61035499-61035518 None:intergenic 45.0%
! TGATGTAATAGTGGAGGTGG+TGG + chr1_2:61035659-61035678 Msa0082240:CDS 45.0%
CGTGCTTCAATCTTGGTTGT+AGG + chr1_2:61035726-61035745 Msa0082240:CDS 45.0%
GTTGTAGGTAGTCACGGTTA+TGG + chr1_2:61035741-61035760 Msa0082240:CDS 45.0%
TGTAGGTAGTCACGGTTATG+GGG + chr1_2:61035743-61035762 Msa0082240:CDS 45.0%
TCTTGTGTTTGTGATCGTGG+TGG - chr1_2:61035928-61035947 None:intergenic 45.0%
! GACTCTTTTAATCTGCAGGG+CGG + chr1_2:61036228-61036247 Msa0082240:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG + chr1_2:61036235-61036254 Msa0082240:intron 45.0%
TGCCAGTATAGATCAGTGCT+TGG - chr1_2:61036326-61036345 None:intergenic 45.0%
CCAACAGAAGAAGTAGCGGT+GGG - chr1_2:61034670-61034689 None:intergenic 50.0%
GAGGCCAACAGAAGAAGTAG+CGG - chr1_2:61034674-61034693 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG - chr1_2:61034693-61034712 None:intergenic 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG + chr1_2:61035663-61035682 Msa0082240:CDS 50.0%
TACTGCTGCCTATCTTCTGC+AGG - chr1_2:61036005-61036024 None:intergenic 50.0%
! GATTGTTGTTGCCTCCTACC+CGG + chr1_2:61036477-61036496 Msa0082240:three_prime_UTR 50.0%
GTAAAATAAGGCCTCCGGGT+AGG - chr1_2:61036494-61036513 None:intergenic 50.0%
CCAACAGAAGAAGTAGCGGT+GGG - chr1_2:61034670-61034689 None:intergenic 50.0%
GAGGCCAACAGAAGAAGTAG+CGG - chr1_2:61034674-61034693 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG - chr1_2:61034693-61034712 None:intergenic 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG + chr1_2:61035663-61035682 Msa0082240:CDS 50.0%
TACTGCTGCCTATCTTCTGC+AGG - chr1_2:61036005-61036024 None:intergenic 50.0%
! GATTGTTGTTGCCTCCTACC+CGG + chr1_2:61036477-61036496 Msa0082240:three_prime_UTR 50.0%
GTAAAATAAGGCCTCCGGGT+AGG - chr1_2:61036494-61036513 None:intergenic 50.0%
CCCACCGCTACTTCTTCTGT+TGG + chr1_2:61034667-61034686 Msa0082240:CDS 55.0%
GCCAACAGAAGAAGTAGCGG+TGG - chr1_2:61034671-61034690 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG + chr1_2:61034676-61034695 Msa0082240:CDS 55.0%
AAATAAGGCCTCCGGGTAGG+AGG - chr1_2:61036491-61036510 None:intergenic 55.0%
CCCACCGCTACTTCTTCTGT+TGG + chr1_2:61034667-61034686 Msa0082240:CDS 55.0%
GCCAACAGAAGAAGTAGCGG+TGG - chr1_2:61034671-61034690 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG + chr1_2:61034676-61034695 Msa0082240:CDS 55.0%
AAATAAGGCCTCCGGGTAGG+AGG - chr1_2:61036491-61036510 None:intergenic 55.0%
AGTGTACGCAGGAGCTGCTG+AGG + chr1_2:61034787-61034806 Msa0082240:intron 60.0%
TGTTGTTGCCTCCTACCCGG+AGG + chr1_2:61036480-61036499 Msa0082240:three_prime_UTR 60.0%
AGTGTACGCAGGAGCTGCTG+AGG + chr1_2:61034787-61034806 Msa0082240:intron 60.0%
TGTTGTTGCCTCCTACCCGG+AGG + chr1_2:61036480-61036499 Msa0082240:three_prime_UTR 60.0%
! TCTGTTGGCCTCGCTGGTCC+TGG + chr1_2:61034682-61034701 Msa0082240:CDS 65.0%
! TCTGTTGGCCTCGCTGGTCC+TGG + chr1_2:61034682-61034701 Msa0082240:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 61034443 61036652 61034443 ID=Msa0082240;Name=Msa0082240
chr1_2 mRNA 61034443 61036652 61034443 ID=Msa0082240-mRNA-1;Parent=Msa0082240;Name=Msa0082240-mRNA-1;_AED=0.04;_eAED=0.04;_QI=62|0.66|0.75|1|1|1|4|319|167
chr1_2 exon 61034443 61034703 61034443 ID=Msa0082240-mRNA-1:exon:20032;Parent=Msa0082240-mRNA-1
chr1_2 exon 61034798 61034892 61034798 ID=Msa0082240-mRNA-1:exon:20033;Parent=Msa0082240-mRNA-1
chr1_2 exon 61035654 61035775 61035654 ID=Msa0082240-mRNA-1:exon:20034;Parent=Msa0082240-mRNA-1
chr1_2 exon 61036246 61036652 61036246 ID=Msa0082240-mRNA-1:exon:20035;Parent=Msa0082240-mRNA-1
chr1_2 five_prime_UTR 61034443 61034504 61034443 ID=Msa0082240-mRNA-1:five_prime_utr;Parent=Msa0082240-mRNA-1
chr1_2 CDS 61034505 61034703 61034505 ID=Msa0082240-mRNA-1:cds;Parent=Msa0082240-mRNA-1
chr1_2 CDS 61034798 61034892 61034798 ID=Msa0082240-mRNA-1:cds;Parent=Msa0082240-mRNA-1
chr1_2 CDS 61035654 61035775 61035654 ID=Msa0082240-mRNA-1:cds;Parent=Msa0082240-mRNA-1
chr1_2 CDS 61036246 61036333 61036246 ID=Msa0082240-mRNA-1:cds;Parent=Msa0082240-mRNA-1
chr1_2 three_prime_UTR 61036334 61036652 61036334 ID=Msa0082240-mRNA-1:three_prime_utr;Parent=Msa0082240-mRNA-1
Gene Sequence

>Msa0082240

ATGGCGAGTGCGAATTCAGAACAATCAACAGAGAAATCACAACCAGTAATGTTAGTTGCAATCGATGAGAGCGATCACAGTGCATACGCTCTGAAATGGACTCTCGATCATTTCTTCAGCACAAACAATCATGTTTTCAAACTTGTTCTTGTTCATGCTAGACCCACCGCTACTTCTTCTGTTGGCCTCGCTGGTCCTGGAGCTGCTGAGGTTTTGCCAATTGTGGATTCTGATTTGAGAAAGATTGCTGCTAGAGTTGCTGAGAATGCTAAACAACTTTGCATTAAGAAATCCGTAAATGATGTAATAGTGGAGGTGGTGGAAGGTGATGCTAGAAATGTTCTCTGTGATACAGTAGAAAAGTATCGTGCTTCAATCTTGGTTGTAGGTAGTCACGGTTATGGGGCTATAAAAAGGGCGGTTTTAGGTAGTGTGAGTGACTACTGTGCTCATCATGCTCATTGCACTGTGATGATTGTGAAGAAGCCAAAAACCAAGCACTGA
Protein sequence

>Msa0082240

MASANSEQSTEKSQPVMLVAIDESDHSAYALKWTLDHFFSTNNHVFKLVLVHARPTATSSVGLAGPGAAEVLPIVDSDLRKIAARVAENAKQLCIKKSVNDVIVEVVEGDARNVLCDTVEKYRASILVVGSHGYGAIKRAVLGSVSDYCAHHAHCTVMIVKKPKTKH*