AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1370820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370820 MtrunA17_Chr1g0195661 95.882 170 4 1 1 170 1 167 3.72e-117 328
Msa1370820 MtrunA17_Chr7g0269301 68.590 156 46 2 16 170 9 162 2.15e-66 199
Msa1370820 MtrunA17_Chr1g0194801 40.252 159 90 3 11 166 2 158 2.67e-34 118
Msa1370820 MtrunA17_Chr1g0176321 40.462 173 79 5 18 170 6 174 1.80e-33 116
Msa1370820 MtrunA17_Chr1g0192651 39.752 161 89 4 10 168 5 159 1.35e-32 114
Msa1370820 MtrunA17_Chr1g0176311 37.805 164 90 3 18 170 25 187 6.08e-31 110
Msa1370820 MtrunA17_Chr5g0427291 32.716 162 96 3 16 164 9 170 2.91e-22 88.2
Msa1370820 MtrunA17_Chr2g0323291 30.189 159 98 4 19 167 29 184 1.86e-16 73.9
Msa1370820 MtrunA17_Chr4g0032261 32.903 155 91 4 19 165 9 158 2.57e-16 72.0
Msa1370820 MtrunA17_Chr4g0001641 49.206 63 32 0 102 164 3 65 1.05e-15 68.2
Msa1370820 MtrunA17_Chr6g0450171 24.051 158 109 3 17 165 8 163 1.31e-12 62.8
Msa1370820 MtrunA17_Chr5g0433951 29.032 155 95 5 19 164 9 157 1.78e-12 62.0
Msa1370820 MtrunA17_Chr2g0294421 29.375 160 100 5 19 170 9 163 3.08e-12 61.2
Msa1370820 MtrunA17_Chr7g0240701 29.299 157 92 5 19 164 9 157 8.72e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370820 AT2G47710.1 62.195 164 57 2 8 169 1 161 8.68e-71 211
Msa1370820 AT3G11930.1 40.449 178 87 6 7 167 20 195 5.95e-34 119
Msa1370820 AT3G11930.2 40.223 179 87 6 7 167 20 196 8.44e-34 118
Msa1370820 AT3G11930.4 40.000 180 87 6 7 167 20 197 1.17e-33 118
Msa1370820 AT1G09740.2 38.365 159 88 5 17 167 10 166 3.55e-28 103
Msa1370820 AT1G09740.1 38.365 159 88 5 17 167 10 166 3.55e-28 103
Msa1370820 AT3G11930.3 35.096 208 83 8 7 167 20 222 1.02e-27 103
Msa1370820 AT3G58450.1 37.952 166 82 5 17 167 32 191 1.02e-26 100
Msa1370820 AT3G62550.1 36.905 168 85 8 7 165 2 157 1.44e-25 96.3
Msa1370820 AT3G58450.2 36.420 162 83 5 17 167 32 184 1.59e-25 97.4
Msa1370820 AT5G49050.1 43.590 117 61 2 13 127 37 150 3.67e-25 95.1
Msa1370820 AT1G11360.2 33.143 175 93 6 10 169 33 198 1.48e-19 82.8
Msa1370820 AT1G11360.1 33.143 175 93 6 10 169 33 198 1.48e-19 82.8
Msa1370820 AT1G11360.4 33.143 175 93 6 10 169 33 198 1.48e-19 82.8
Msa1370820 AT1G11360.3 33.143 175 93 6 10 169 33 198 1.48e-19 82.8
Msa1370820 AT1G68300.1 32.278 158 100 4 11 164 3 157 2.73e-18 77.4
Msa1370820 AT5G14680.1 27.647 170 99 5 12 165 2 163 4.14e-16 72.4
Msa1370820 AT3G01520.1 28.676 136 95 2 30 165 30 163 7.52e-15 68.9
Msa1370820 AT3G17020.1 28.477 151 102 2 19 164 11 160 8.54e-13 63.2
Msa1370820 AT3G53990.1 25.325 154 102 4 19 164 9 157 2.71e-12 61.6
Msa1370820 AT2G21620.2 30.612 147 85 4 17 163 42 171 1.99e-11 60.1
Msa1370820 AT2G21620.1 29.932 147 80 4 17 163 42 165 2.06e-11 60.1

Find 41 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTGATACTTTATGAAAA+TGG 0.199567 tig0019143:+128786 Msa1370820:three_prime_UTR
TTCATATACAAAGTAAAATA+AGG 0.268743 tig0019143:-128704 None:intergenic
TTGTAGGTAGTCACGGTTAT+GGG 0.301582 tig0019143:+127958 Msa1370820:CDS
AGTTTATCCTATGGCATGTT+TGG 0.308645 tig0019143:+128620 Msa1370820:three_prime_UTR
TCGATTGCAACTAACATTAC+TGG 0.334213 tig0019143:-126852 None:intergenic
GTTGTAGGTAGTCACGGTTA+TGG 0.361010 tig0019143:+127957 Msa1370820:CDS
CGTGCTTCAATCTTGGTTGT+AGG 0.387207 tig0019143:+127942 Msa1370820:CDS
CTCAAATCAGAATCCACAAT+TGG 0.390759 tig0019143:-127121 None:intergenic
AAAGTATCGTGCTTCAATCT+TGG 0.402940 tig0019143:+127935 Msa1370820:CDS
ACTTCTTCTGTTGGCCTCGC+TGG 0.403590 tig0019143:+126981 Msa1370820:CDS
ACAGTGCATACGCTCTGAAA+TGG 0.408844 tig0019143:+126886 Msa1370820:CDS
TCTGTTGGCCTCGCTGGTCC+TGG 0.423133 tig0019143:+126987 Msa1370820:CDS
GATTATATATTTATTCTTGT+AGG 0.425496 tig0019143:-128550 None:intergenic
AACCAAGCACTGATCTATAC+TGG 0.427898 tig0019143:+128522 Msa1370820:exon
ACGGTTATGGGGCTATAAAA+AGG 0.451906 tig0019143:+127970 Msa1370820:CDS
ACAAAGTAAAATAAGGCCTC+CGG 0.457393 tig0019143:-128697 None:intergenic
TTGTGACTTAGTTTATCCTA+TGG 0.465564 tig0019143:+128611 Msa1370820:three_prime_UTR
ATACTTTATGAAAATGGCAA+TGG 0.499210 tig0019143:+128792 Msa1370820:three_prime_UTR
GCTATTTCACTTTAGCTGAA+AGG 0.508675 tig0019143:-128572 None:intergenic
TCAGGTAAATGATGTAATAG+TGG 0.511125 tig0019143:+127866 Msa1370820:intron
ATCTTGGTTGTAGGTAGTCA+CGG 0.516671 tig0019143:+127951 Msa1370820:CDS
CCCACCGCTACTTCTTCTGT+TGG 0.524480 tig0019143:+126972 Msa1370820:CDS
CAAAGTAAAATAAGGCCTCC+GGG 0.542553 tig0019143:-128696 None:intergenic
TGCCAGTATAGATCAGTGCT+TGG 0.553700 tig0019143:-128524 None:intergenic
TGATGTAATAGTGGAGGTGG+TGG 0.556420 tig0019143:+127875 Msa1370820:CDS
AAATAAGGCCTCCGGGTAGG+AGG 0.557118 tig0019143:-128689 None:intergenic
AAATGATGTAATAGTGGAGG+TGG 0.562868 tig0019143:+127872 Msa1370820:CDS
GATTGTTGTTGCCTCCTACC+CGG 0.564104 tig0019143:+128678 Msa1370820:three_prime_UTR
GTAAAATAAGGCCTCCGGGT+AGG 0.586447 tig0019143:-128692 None:intergenic
CTACAAACCAAACATGCCAT+AGG 0.591562 tig0019143:-128627 None:intergenic
AGTGTACGCAGGAGCTGCTG+AGG 0.594737 tig0019143:+127093 Msa1370820:intron
TGTAGGTAGTCACGGTTATG+GGG 0.599167 tig0019143:+127959 Msa1370820:CDS
ATAGAAAATATGCAATTGTG+TGG 0.605193 tig0019143:+128656 Msa1370820:three_prime_UTR
GCCAACAGAAGAAGTAGCGG+TGG 0.605329 tig0019143:-126973 None:intergenic
TTGTGCTAATTAGTGTACGC+AGG 0.608881 tig0019143:+127082 Msa1370820:intron
GTAATAGTGGAGGTGGTGGA+AGG 0.626493 tig0019143:+127879 Msa1370820:CDS
GGTAAATGATGTAATAGTGG+AGG 0.637317 tig0019143:+127869 Msa1370820:intron
GAGGCCAACAGAAGAAGTAG+CGG 0.655250 tig0019143:-126976 None:intergenic
CCAACAGAAGAAGTAGCGGT+GGG 0.684778 tig0019143:-126972 None:intergenic
TGTTGTTGCCTCCTACCCGG+AGG 0.694844 tig0019143:+128681 Msa1370820:three_prime_UTR
GAATTATACCAGGACCAGCG+AGG 0.726201 tig0019143:-126995 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTATGAAAAGAATTA+GGG - tig0019143:127298-127317 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG - tig0019143:127299-127318 None:intergenic 10.0%
!!! TTTCATAATTTTATTTGATA+AGG + tig0019143:127305-127324 Msa1370820:intron 10.0%
!!! ATGATATTATTTTAGATAAT+AGG + tig0019143:127431-127450 Msa1370820:intron 10.0%
!! TAAAATTATGAAAAGAATTA+GGG - tig0019143:127298-127317 None:intergenic 10.0%
!! ATAAAATTATGAAAAGAATT+AGG - tig0019143:127299-127318 None:intergenic 10.0%
!!! TTTCATAATTTTATTTGATA+AGG + tig0019143:127305-127324 Msa1370820:intron 10.0%
!!! ATGATATTATTTTAGATAAT+AGG + tig0019143:127431-127450 Msa1370820:intron 10.0%
!!! ATTTTATTTGATAAGGAAAA+TGG + tig0019143:127312-127331 Msa1370820:intron 15.0%
!! AAAAATGTTAGAATTAAATG+CGG - tig0019143:128093-128112 None:intergenic 15.0%
!!! ATTACATTTATTCATGTTTT+TGG - tig0019143:128160-128179 None:intergenic 15.0%
!! AAATAAACTTAAAAAGCAAA+AGG - tig0019143:128310-128329 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG + tig0019143:128331-128350 Msa1370820:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG + tig0019143:128332-128351 Msa1370820:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG + tig0019143:128333-128352 Msa1370820:intron 15.0%
!! GATTATATATTTATTCTTGT+AGG - tig0019143:128553-128572 None:intergenic 15.0%
!! TTCATATACAAAGTAAAATA+AGG - tig0019143:128707-128726 None:intergenic 15.0%
!! TTGAAAAATAATCATTGTTA+AGG + tig0019143:128835-128854 Msa1370820:three_prime_UTR 15.0%
!!! ATTTTATTTGATAAGGAAAA+TGG + tig0019143:127312-127331 Msa1370820:intron 15.0%
!! AAAAATGTTAGAATTAAATG+CGG - tig0019143:128093-128112 None:intergenic 15.0%
!!! ATTACATTTATTCATGTTTT+TGG - tig0019143:128160-128179 None:intergenic 15.0%
!! AAATAAACTTAAAAAGCAAA+AGG - tig0019143:128310-128329 None:intergenic 15.0%
!!! ACTTTTCATTTTATTTATAC+TGG + tig0019143:128331-128350 Msa1370820:intron 15.0%
!!! CTTTTCATTTTATTTATACT+GGG + tig0019143:128332-128351 Msa1370820:intron 15.0%
!!! TTTTCATTTTATTTATACTG+GGG + tig0019143:128333-128352 Msa1370820:intron 15.0%
!! GATTATATATTTATTCTTGT+AGG - tig0019143:128553-128572 None:intergenic 15.0%
!! TTCATATACAAAGTAAAATA+AGG - tig0019143:128707-128726 None:intergenic 15.0%
!!! TTTGTTGATGTTTTGATAAT+TGG + tig0019143:127050-127069 Msa1370820:intron 20.0%
!! AACAAAAATAGAAACACATA+CGG - tig0019143:127200-127219 None:intergenic 20.0%
!!! TTTTTTGTTAGTTTGATGAA+AGG + tig0019143:127543-127562 Msa1370820:intron 20.0%
!!! TTTCATTTTATTTATACTGG+GGG + tig0019143:128334-128353 Msa1370820:intron 20.0%
!!! TTTGTTGATGTTTTGATAAT+TGG + tig0019143:127050-127069 Msa1370820:intron 20.0%
!! AACAAAAATAGAAACACATA+CGG - tig0019143:127200-127219 None:intergenic 20.0%
!!! TTTTTTGTTAGTTTGATGAA+AGG + tig0019143:127543-127562 Msa1370820:intron 20.0%
!!! TTTCATTTTATTTATACTGG+GGG + tig0019143:128334-128353 Msa1370820:intron 20.0%
! ATGAAATTGAGAATTATACC+AGG - tig0019143:127008-127027 None:intergenic 25.0%
!!! TGAATTTTCTCTGGTTTATA+CGG + tig0019143:127347-127366 Msa1370820:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG - tig0019143:127736-127755 None:intergenic 25.0%
! TTAAGTACTTATGTCATATG+TGG + tig0019143:127817-127836 Msa1370820:intron 25.0%
! AAACTTAAAAAGCAAAAGGA+AGG - tig0019143:128306-128325 None:intergenic 25.0%
! ATAGAAAATATGCAATTGTG+TGG + tig0019143:128656-128675 Msa1370820:three_prime_UTR 25.0%
!! GCAAAAAACAGTAATTTTTC+AGG - tig0019143:128768-128787 None:intergenic 25.0%
! ATACTTTATGAAAATGGCAA+TGG + tig0019143:128792-128811 Msa1370820:three_prime_UTR 25.0%
! ATGAAATTGAGAATTATACC+AGG - tig0019143:127008-127027 None:intergenic 25.0%
!!! TGAATTTTCTCTGGTTTATA+CGG + tig0019143:127347-127366 Msa1370820:intron 25.0%
!!! TTAATACACACAAGTTTTGT+TGG - tig0019143:127736-127755 None:intergenic 25.0%
! TTAAGTACTTATGTCATATG+TGG + tig0019143:127817-127836 Msa1370820:intron 25.0%
! AAACTTAAAAAGCAAAAGGA+AGG - tig0019143:128306-128325 None:intergenic 25.0%
! ATAGAAAATATGCAATTGTG+TGG + tig0019143:128656-128675 Msa1370820:three_prime_UTR 25.0%
!! GCAAAAAACAGTAATTTTTC+AGG - tig0019143:128768-128787 None:intergenic 25.0%
! ATACTTTATGAAAATGGCAA+TGG + tig0019143:128792-128811 Msa1370820:three_prime_UTR 25.0%
!! TCCGTTAGTGATTTTTAGAA+TGG + tig0019143:126791-126810 Msa1370820:exon 30.0%
! GCCATTCTAAAAATCACTAA+CGG - tig0019143:126795-126814 None:intergenic 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG + tig0019143:127323-127342 Msa1370820:intron 30.0%
! TTGAATGGATGAATTTTCTC+TGG + tig0019143:127338-127357 Msa1370820:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG + tig0019143:127370-127389 Msa1370820:intron 30.0%
CCTGTATTTATATGTTGTGT+GGG + tig0019143:127371-127390 Msa1370820:intron 30.0%
GATGAAAGGAGATAAATTGA+AGG + tig0019143:127557-127576 Msa1370820:intron 30.0%
TTACCATCATCTTTGTATGT+CGG + tig0019143:127657-127676 Msa1370820:intron 30.0%
!! TTTTTCTACTTTCGAGTCTA+AGG + tig0019143:127772-127791 Msa1370820:intron 30.0%
! TCTGATAACTGAATTTTCTC+AGG + tig0019143:127848-127867 Msa1370820:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG + tig0019143:127866-127885 Msa1370820:intron 30.0%
TACAATTATCATCCTTCGTT+TGG + tig0019143:128010-128029 Msa1370820:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG + tig0019143:128051-128070 Msa1370820:intron 30.0%
AATTAAATGCGGCATATTCA+TGG - tig0019143:128082-128101 None:intergenic 30.0%
! TAATTTTGTGTTTGTGATCG+TGG - tig0019143:128132-128151 None:intergenic 30.0%
TTGTGACTTAGTTTATCCTA+TGG + tig0019143:128611-128630 Msa1370820:three_prime_UTR 30.0%
GCCTTGATACTTTATGAAAA+TGG + tig0019143:128786-128805 Msa1370820:three_prime_UTR 30.0%
!! GCCATTTTCATAAAGTATCA+AGG - tig0019143:128790-128809 None:intergenic 30.0%
!! TCCGTTAGTGATTTTTAGAA+TGG + tig0019143:126791-126810 Msa1370820:exon 30.0%
! GCCATTCTAAAAATCACTAA+CGG - tig0019143:126795-126814 None:intergenic 30.0%
! TAAGGAAAATGGCTTTTGAA+TGG + tig0019143:127323-127342 Msa1370820:intron 30.0%
! TTGAATGGATGAATTTTCTC+TGG + tig0019143:127338-127357 Msa1370820:intron 30.0%
TCCTGTATTTATATGTTGTG+TGG + tig0019143:127370-127389 Msa1370820:intron 30.0%
CCTGTATTTATATGTTGTGT+GGG + tig0019143:127371-127390 Msa1370820:intron 30.0%
GATGAAAGGAGATAAATTGA+AGG + tig0019143:127557-127576 Msa1370820:intron 30.0%
TTACCATCATCTTTGTATGT+CGG + tig0019143:127657-127676 Msa1370820:intron 30.0%
!! TTTTTCTACTTTCGAGTCTA+AGG + tig0019143:127772-127791 Msa1370820:intron 30.0%
! TCTGATAACTGAATTTTCTC+AGG + tig0019143:127848-127867 Msa1370820:intron 30.0%
TCAGGTAAATGATGTAATAG+TGG + tig0019143:127866-127885 Msa1370820:intron 30.0%
TACAATTATCATCCTTCGTT+TGG + tig0019143:128010-128029 Msa1370820:intron 30.0%
!!! CTTTTCTGAAACTTGTGAAT+AGG + tig0019143:128051-128070 Msa1370820:intron 30.0%
AATTAAATGCGGCATATTCA+TGG - tig0019143:128082-128101 None:intergenic 30.0%
! TAATTTTGTGTTTGTGATCG+TGG - tig0019143:128132-128151 None:intergenic 30.0%
TTGTGACTTAGTTTATCCTA+TGG + tig0019143:128611-128630 Msa1370820:three_prime_UTR 30.0%
GCCTTGATACTTTATGAAAA+TGG + tig0019143:128786-128805 Msa1370820:three_prime_UTR 30.0%
!! GCCATTTTCATAAAGTATCA+AGG - tig0019143:128790-128809 None:intergenic 30.0%
TCGATTGCAACTAACATTAC+TGG - tig0019143:126855-126874 None:intergenic 35.0%
CTCAAATCAGAATCCACAAT+TGG - tig0019143:127124-127143 None:intergenic 35.0%
CCCACACAACATATAAATAC+AGG - tig0019143:127374-127393 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG + tig0019143:127377-127396 Msa1370820:intron 35.0%
TCCACTTAAATTGCTTCAAC+TGG + tig0019143:127623-127642 Msa1370820:intron 35.0%
ACCAGTTGAAGCAATTTAAG+TGG - tig0019143:127627-127646 None:intergenic 35.0%
TAACCGACATACAAAGATGA+TGG - tig0019143:127663-127682 None:intergenic 35.0%
! TCGGTTAATTGTCATGCTTT+TGG + tig0019143:127676-127695 Msa1370820:intron 35.0%
GGTAAATGATGTAATAGTGG+AGG + tig0019143:127869-127888 Msa1370820:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG + tig0019143:127872-127891 Msa1370820:CDS 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG + tig0019143:127935-127954 Msa1370820:CDS 35.0%
TATGGGGCTATAAAAAGGTA+TGG + tig0019143:127975-127994 Msa1370820:intron 35.0%
! AAAAAGGTATGGCTTCATTG+TGG + tig0019143:127986-128005 Msa1370820:intron 35.0%
TTACATATCACTCCAAACGA+AGG - tig0019143:128025-128044 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG - tig0019143:128186-128205 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG + tig0019143:128195-128214 Msa1370820:intron 35.0%
CAAAAGGAAGGTTTGATTGT+TGG - tig0019143:128294-128313 None:intergenic 35.0%
!! ATAGATCAGTGCTTGGTTTT+TGG - tig0019143:128520-128539 None:intergenic 35.0%
! GCTATTTCACTTTAGCTGAA+AGG - tig0019143:128575-128594 None:intergenic 35.0%
AGTTTATCCTATGGCATGTT+TGG + tig0019143:128620-128639 Msa1370820:three_prime_UTR 35.0%
ACAAAGTAAAATAAGGCCTC+CGG - tig0019143:128700-128719 None:intergenic 35.0%
TCGATTGCAACTAACATTAC+TGG - tig0019143:126855-126874 None:intergenic 35.0%
CTCAAATCAGAATCCACAAT+TGG - tig0019143:127124-127143 None:intergenic 35.0%
CCCACACAACATATAAATAC+AGG - tig0019143:127374-127393 None:intergenic 35.0%
TTTATATGTTGTGTGGGACT+TGG + tig0019143:127377-127396 Msa1370820:intron 35.0%
TCCACTTAAATTGCTTCAAC+TGG + tig0019143:127623-127642 Msa1370820:intron 35.0%
ACCAGTTGAAGCAATTTAAG+TGG - tig0019143:127627-127646 None:intergenic 35.0%
TAACCGACATACAAAGATGA+TGG - tig0019143:127663-127682 None:intergenic 35.0%
! TCGGTTAATTGTCATGCTTT+TGG + tig0019143:127676-127695 Msa1370820:intron 35.0%
GGTAAATGATGTAATAGTGG+AGG + tig0019143:127869-127888 Msa1370820:intron 35.0%
! AAATGATGTAATAGTGGAGG+TGG + tig0019143:127872-127891 Msa1370820:CDS 35.0%
!! AAAGTATCGTGCTTCAATCT+TGG + tig0019143:127935-127954 Msa1370820:CDS 35.0%
TATGGGGCTATAAAAAGGTA+TGG + tig0019143:127975-127994 Msa1370820:intron 35.0%
! AAAAAGGTATGGCTTCATTG+TGG + tig0019143:127986-128005 Msa1370820:intron 35.0%
TTACATATCACTCCAAACGA+AGG - tig0019143:128025-128044 None:intergenic 35.0%
AGGTTAAAGATTTCAGTGGA+TGG - tig0019143:128186-128205 None:intergenic 35.0%
ATCTTTAACCTGCAGAAGAT+AGG + tig0019143:128195-128214 Msa1370820:intron 35.0%
CAAAAGGAAGGTTTGATTGT+TGG - tig0019143:128294-128313 None:intergenic 35.0%
!! ATAGATCAGTGCTTGGTTTT+TGG - tig0019143:128520-128539 None:intergenic 35.0%
! GCTATTTCACTTTAGCTGAA+AGG - tig0019143:128575-128594 None:intergenic 35.0%
AGTTTATCCTATGGCATGTT+TGG + tig0019143:128620-128639 Msa1370820:three_prime_UTR 35.0%
ACAAAGTAAAATAAGGCCTC+CGG - tig0019143:128700-128719 None:intergenic 35.0%
TTGTGCTAATTAGTGTACGC+AGG + tig0019143:127082-127101 Msa1370820:intron 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG + tig0019143:127951-127970 Msa1370820:CDS 40.0%
TTGTAGGTAGTCACGGTTAT+GGG + tig0019143:127958-127977 Msa1370820:CDS 40.0%
ACGGTTATGGGGCTATAAAA+AGG + tig0019143:127970-127989 Msa1370820:CDS 40.0%
! TTTTGTGTTTGTGATCGTGG+TGG - tig0019143:128129-128148 None:intergenic 40.0%
CTGCAGGTTAAAGATTTCAG+TGG - tig0019143:128190-128209 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG - tig0019143:128411-128430 None:intergenic 40.0%
! ACTCGACTCTTTTAATCTGC+AGG + tig0019143:128425-128444 Msa1370820:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG + tig0019143:128426-128445 Msa1370820:intron 40.0%
!! AACCAAGCACTGATCTATAC+TGG + tig0019143:128522-128541 Msa1370820:exon 40.0%
CTACAAACCAAACATGCCAT+AGG - tig0019143:128630-128649 None:intergenic 40.0%
CAAAGTAAAATAAGGCCTCC+GGG - tig0019143:128699-128718 None:intergenic 40.0%
TTGTGCTAATTAGTGTACGC+AGG + tig0019143:127082-127101 Msa1370820:intron 40.0%
ATCTTGGTTGTAGGTAGTCA+CGG + tig0019143:127951-127970 Msa1370820:CDS 40.0%
TTGTAGGTAGTCACGGTTAT+GGG + tig0019143:127958-127977 Msa1370820:CDS 40.0%
ACGGTTATGGGGCTATAAAA+AGG + tig0019143:127970-127989 Msa1370820:CDS 40.0%
! TTTTGTGTTTGTGATCGTGG+TGG - tig0019143:128129-128148 None:intergenic 40.0%
CTGCAGGTTAAAGATTTCAG+TGG - tig0019143:128190-128209 None:intergenic 40.0%
TCGAGTGTAGTGAGTGAATT+AGG - tig0019143:128411-128430 None:intergenic 40.0%
! ACTCGACTCTTTTAATCTGC+AGG + tig0019143:128425-128444 Msa1370820:intron 40.0%
! CTCGACTCTTTTAATCTGCA+GGG + tig0019143:128426-128445 Msa1370820:intron 40.0%
!! AACCAAGCACTGATCTATAC+TGG + tig0019143:128522-128541 Msa1370820:exon 40.0%
CTACAAACCAAACATGCCAT+AGG - tig0019143:128630-128649 None:intergenic 40.0%
CAAAGTAAAATAAGGCCTCC+GGG - tig0019143:128699-128718 None:intergenic 40.0%
!! ACAGTGCATACGCTCTGAAA+TGG + tig0019143:126886-126905 Msa1370820:CDS 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG + tig0019143:127108-127127 Msa1370820:CDS 45.0%
GGGACTTGGAGCATACTATA+TGG + tig0019143:127391-127410 Msa1370820:intron 45.0%
TAGCTGAATGCATCATGCTC+AGG + tig0019143:127468-127487 Msa1370820:intron 45.0%
AGTTCCATACCCACATCCTT+AGG + tig0019143:127709-127728 Msa1370820:intron 45.0%
GTTCCATACCCACATCCTTA+GGG + tig0019143:127710-127729 Msa1370820:intron 45.0%
TTTGTTGGCCCTAAGGATGT+GGG - tig0019143:127721-127740 None:intergenic 45.0%
! TTTTGTTGGCCCTAAGGATG+TGG - tig0019143:127722-127741 None:intergenic 45.0%
!!! CACAAGTTTTGTTGGCCCTA+AGG - tig0019143:127728-127747 None:intergenic 45.0%
! TGATGTAATAGTGGAGGTGG+TGG + tig0019143:127875-127894 Msa1370820:CDS 45.0%
CGTGCTTCAATCTTGGTTGT+AGG + tig0019143:127942-127961 Msa1370820:CDS 45.0%
GTTGTAGGTAGTCACGGTTA+TGG + tig0019143:127957-127976 Msa1370820:CDS 45.0%
TGTAGGTAGTCACGGTTATG+GGG + tig0019143:127959-127978 Msa1370820:CDS 45.0%
! GACTCTTTTAATCTGCAGGG+CGG + tig0019143:128429-128448 Msa1370820:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG + tig0019143:128436-128455 Msa1370820:intron 45.0%
TGCCAGTATAGATCAGTGCT+TGG - tig0019143:128527-128546 None:intergenic 45.0%
!! ACAGTGCATACGCTCTGAAA+TGG + tig0019143:126886-126905 Msa1370820:CDS 45.0%
! TGCTGAGGTTTTGCCAATTG+TGG + tig0019143:127108-127127 Msa1370820:CDS 45.0%
GGGACTTGGAGCATACTATA+TGG + tig0019143:127391-127410 Msa1370820:intron 45.0%
TAGCTGAATGCATCATGCTC+AGG + tig0019143:127468-127487 Msa1370820:intron 45.0%
AGTTCCATACCCACATCCTT+AGG + tig0019143:127709-127728 Msa1370820:intron 45.0%
GTTCCATACCCACATCCTTA+GGG + tig0019143:127710-127729 Msa1370820:intron 45.0%
TTTGTTGGCCCTAAGGATGT+GGG - tig0019143:127721-127740 None:intergenic 45.0%
! TTTTGTTGGCCCTAAGGATG+TGG - tig0019143:127722-127741 None:intergenic 45.0%
!!! CACAAGTTTTGTTGGCCCTA+AGG - tig0019143:127728-127747 None:intergenic 45.0%
! TGATGTAATAGTGGAGGTGG+TGG + tig0019143:127875-127894 Msa1370820:CDS 45.0%
CGTGCTTCAATCTTGGTTGT+AGG + tig0019143:127942-127961 Msa1370820:CDS 45.0%
GTTGTAGGTAGTCACGGTTA+TGG + tig0019143:127957-127976 Msa1370820:CDS 45.0%
TGTAGGTAGTCACGGTTATG+GGG + tig0019143:127959-127978 Msa1370820:CDS 45.0%
! GACTCTTTTAATCTGCAGGG+CGG + tig0019143:128429-128448 Msa1370820:intron 45.0%
! TTAATCTGCAGGGCGGTTTT+AGG + tig0019143:128436-128455 Msa1370820:intron 45.0%
TGCCAGTATAGATCAGTGCT+TGG - tig0019143:128527-128546 None:intergenic 45.0%
CCAACAGAAGAAGTAGCGGT+GGG - tig0019143:126975-126994 None:intergenic 50.0%
GAGGCCAACAGAAGAAGTAG+CGG - tig0019143:126979-126998 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG - tig0019143:126998-127017 None:intergenic 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG + tig0019143:127879-127898 Msa1370820:CDS 50.0%
TACTGCTGCCTATCTTCTGC+AGG - tig0019143:128206-128225 None:intergenic 50.0%
! GATTGTTGTTGCCTCCTACC+CGG + tig0019143:128678-128697 Msa1370820:three_prime_UTR 50.0%
GTAAAATAAGGCCTCCGGGT+AGG - tig0019143:128695-128714 None:intergenic 50.0%
CCAACAGAAGAAGTAGCGGT+GGG - tig0019143:126975-126994 None:intergenic 50.0%
GAGGCCAACAGAAGAAGTAG+CGG - tig0019143:126979-126998 None:intergenic 50.0%
GAATTATACCAGGACCAGCG+AGG - tig0019143:126998-127017 None:intergenic 50.0%
!! GTAATAGTGGAGGTGGTGGA+AGG + tig0019143:127879-127898 Msa1370820:CDS 50.0%
TACTGCTGCCTATCTTCTGC+AGG - tig0019143:128206-128225 None:intergenic 50.0%
! GATTGTTGTTGCCTCCTACC+CGG + tig0019143:128678-128697 Msa1370820:three_prime_UTR 50.0%
GTAAAATAAGGCCTCCGGGT+AGG - tig0019143:128695-128714 None:intergenic 50.0%
CCCACCGCTACTTCTTCTGT+TGG + tig0019143:126972-126991 Msa1370820:CDS 55.0%
GCCAACAGAAGAAGTAGCGG+TGG - tig0019143:126976-126995 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG + tig0019143:126981-127000 Msa1370820:CDS 55.0%
TGGCCCTAAGGATGTGGGTA+TGG - tig0019143:127716-127735 None:intergenic 55.0%
AAATAAGGCCTCCGGGTAGG+AGG - tig0019143:128692-128711 None:intergenic 55.0%
CCCACCGCTACTTCTTCTGT+TGG + tig0019143:126972-126991 Msa1370820:CDS 55.0%
GCCAACAGAAGAAGTAGCGG+TGG - tig0019143:126976-126995 None:intergenic 55.0%
! ACTTCTTCTGTTGGCCTCGC+TGG + tig0019143:126981-127000 Msa1370820:CDS 55.0%
TGGCCCTAAGGATGTGGGTA+TGG - tig0019143:127716-127735 None:intergenic 55.0%
AAATAAGGCCTCCGGGTAGG+AGG - tig0019143:128692-128711 None:intergenic 55.0%
AGTGTACGCAGGAGCTGCTG+AGG + tig0019143:127093-127112 Msa1370820:intron 60.0%
TGTTGTTGCCTCCTACCCGG+AGG + tig0019143:128681-128700 Msa1370820:three_prime_UTR 60.0%
AGTGTACGCAGGAGCTGCTG+AGG + tig0019143:127093-127112 Msa1370820:intron 60.0%
TGTTGTTGCCTCCTACCCGG+AGG + tig0019143:128681-128700 Msa1370820:three_prime_UTR 60.0%
! TCTGTTGGCCTCGCTGGTCC+TGG + tig0019143:126987-127006 Msa1370820:CDS 65.0%
! TCTGTTGGCCTCGCTGGTCC+TGG + tig0019143:126987-127006 Msa1370820:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0019143 gene 126748 128855 126748 ID=Msa1370820;Name=Msa1370820
tig0019143 mRNA 126748 128855 126748 ID=Msa1370820-mRNA-1;Parent=Msa1370820;Name=Msa1370820-mRNA-1;_AED=0.05;_eAED=0.05;_QI=62|1|1|1|0.66|0.75|4|321|170
tig0019143 exon 126748 127008 126748 ID=Msa1370820-mRNA-1:exon:1804;Parent=Msa1370820-mRNA-1
tig0019143 exon 127095 127198 127095 ID=Msa1370820-mRNA-1:exon:1805;Parent=Msa1370820-mRNA-1
tig0019143 exon 127870 127991 127870 ID=Msa1370820-mRNA-1:exon:1806;Parent=Msa1370820-mRNA-1
tig0019143 exon 128447 128855 128447 ID=Msa1370820-mRNA-1:exon:1807;Parent=Msa1370820-mRNA-1
tig0019143 five_prime_UTR 126748 126809 126748 ID=Msa1370820-mRNA-1:five_prime_utr;Parent=Msa1370820-mRNA-1
tig0019143 CDS 126810 127008 126810 ID=Msa1370820-mRNA-1:cds;Parent=Msa1370820-mRNA-1
tig0019143 CDS 127095 127198 127095 ID=Msa1370820-mRNA-1:cds;Parent=Msa1370820-mRNA-1
tig0019143 CDS 127870 127991 127870 ID=Msa1370820-mRNA-1:cds;Parent=Msa1370820-mRNA-1
tig0019143 CDS 128447 128534 128447 ID=Msa1370820-mRNA-1:cds;Parent=Msa1370820-mRNA-1
tig0019143 three_prime_UTR 128535 128855 128535 ID=Msa1370820-mRNA-1:three_prime_utr;Parent=Msa1370820-mRNA-1
Gene Sequence

>Msa1370820

ATGGCGAGTGCGAATTCAGAACAATCAACAGAGAAATCACAACCAGTAATGTTAGTTGCAATCGATGAGAGCGATCACAGTGCATACGCTCTGAAATGGACTCTCGATCATTTCTTCAGCACAAACAATCATGTTTTCAAACTTGTTCTTGTTCATGCTAGACCCACCGCTACTTCTTCTGTTGGCCTCGCTGGTCCTGTGTACGCAGGAGCTGCTGAGGTTTTGCCAATTGTGGATTCTGATTTGAGAAAGATTGCTGCTAGAGTTGCTGAGAATGCTAAACAACTTTGCATTAAGAAATCCGTAAATGATGTAATAGTGGAGGTGGTGGAAGGTGATGCTAGAAATGTTCTCTGTGATACAGTAGAAAAGTATCGTGCTTCAATCTTGGTTGTAGGTAGTCACGGTTATGGGGCTATAAAAAGGGCGGTTTTAGGTAGTGTGAGTGACTACTGTGCTCATCATGCTCATTGCACTGTGATGATTGTGAAGAAGCCAAAAACCAAGCACTGA
Protein sequence

>Msa1370820

MASANSEQSTEKSQPVMLVAIDESDHSAYALKWTLDHFFSTNNHVFKLVLVHARPTATSSVGLAGPVYAGAAEVLPIVDSDLRKIAARVAENAKQLCIKKSVNDVIVEVVEGDARNVLCDTVEKYRASILVVGSHGYGAIKRAVLGSVSDYCAHHAHCTVMIVKKPKTKH*