AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0131610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0131610 MtrunA17_Chr1g0192471 97.400 423 11 0 1 423 1 423 0.0 847
Msa0131610 MtrunA17_Chr7g0271971 85.071 422 61 2 1 422 1 420 0.0 717
Msa0131610 MtrunA17_Chr1g0209451 68.396 424 130 3 1 422 1 422 0.0 589
Msa0131610 MtrunA17_Chr1g0153671 56.341 410 177 2 14 423 11 418 1.92e-168 479
Msa0131610 MtrunA17_Chr7g0225501 62.442 434 138 13 1 422 19 439 1.71e-166 474
Msa0131610 MtrunA17_Chr3g0133801 55.556 414 175 3 14 422 10 419 4.61e-163 465
Msa0131610 MtrunA17_Chr2g0326251 64.486 214 61 8 109 317 7 210 9.41e-74 230
Msa0131610 MtrunA17_Chr1g0150451 31.449 283 155 12 25 298 72 324 1.57e-28 114
Msa0131610 MtrunA17_Chr3g0136711 30.924 249 144 9 57 298 54 281 3.39e-27 110
Msa0131610 MtrunA17_Chr1g0212961 31.673 281 155 14 25 299 90 339 1.44e-25 106
Msa0131610 MtrunA17_Chr3g0105351 29.779 272 163 11 33 297 64 314 3.95e-25 104
Msa0131610 MtrunA17_Chr3g0105371 30.037 273 161 10 33 297 88 338 5.29e-25 105
Msa0131610 MtrunA17_Chr5g0430481 28.625 269 164 11 36 297 127 374 1.05e-22 99.0
Msa0131610 MtrunA17_Chr8g0360351 30.043 233 139 8 68 295 66 279 3.24e-21 93.2
Msa0131610 MtrunA17_Chr2g0317021 29.572 257 152 11 46 297 100 332 2.93e-20 91.3
Msa0131610 MtrunA17_Chr4g0070871 27.365 296 159 10 46 297 220 503 4.07e-20 92.4
Msa0131610 MtrunA17_Chr1g0171571 31.301 246 136 7 69 299 419 646 2.31e-19 90.5
Msa0131610 MtrunA17_Chr3g0126511 29.020 255 152 9 46 295 108 338 2.77e-19 89.0
Msa0131610 MtrunA17_Chr4g0050381 27.338 278 169 9 27 295 21 274 4.50e-19 86.7
Msa0131610 MtrunA17_Chr3g0115851 29.032 279 163 11 27 295 26 279 2.97e-18 84.7
Msa0131610 MtrunA17_Chr5g0407161 26.894 264 153 9 67 314 123 362 4.04e-18 85.5
Msa0131610 MtrunA17_Chr2g0279151 25.818 275 177 9 27 295 25 278 4.20e-18 84.0
Msa0131610 MtrunA17_Chr5g0426141 29.749 279 161 12 27 295 26 279 8.66e-18 83.2
Msa0131610 MtrunA17_Chr6g0466641 87.805 41 5 0 1 41 1 41 1.04e-16 75.1
Msa0131610 MtrunA17_Chr5g0396731 29.048 210 124 7 66 265 105 299 1.08e-16 80.9
Msa0131610 MtrunA17_Chr4g0051661 29.921 254 150 10 46 295 166 395 1.20e-16 81.3
Msa0131610 MtrunA17_Chr8g0389921 29.442 197 118 6 69 258 199 381 2.12e-16 80.9
Msa0131610 MtrunA17_Chr4g0065141 29.644 253 131 11 65 295 121 348 2.93e-16 80.1
Msa0131610 MtrunA17_Chr2g0299981 25.857 321 162 10 40 297 228 535 3.58e-16 80.5
Msa0131610 MtrunA17_Chr1g0150781 29.520 271 135 12 69 299 291 545 3.94e-16 80.5
Msa0131610 MtrunA17_Chr2g0293811 31.076 251 137 10 64 295 96 329 7.50e-16 78.6
Msa0131610 MtrunA17_Chr3g0112291 28.571 217 123 6 62 261 117 318 9.75e-16 78.6
Msa0131610 MtrunA17_Chr1g0194191 29.213 267 152 12 67 313 117 366 1.59e-15 77.8
Msa0131610 MtrunA17_Chr6g0483411 27.723 303 166 13 26 295 34 316 1.74e-15 77.0
Msa0131610 MtrunA17_Chr3g0135961 27.206 272 150 8 62 297 280 539 5.51e-15 77.0
Msa0131610 MtrunA17_Chr1g0158781 31.048 248 138 9 69 297 146 379 7.40e-15 75.9
Msa0131610 MtrunA17_Chr7g0272061 33.898 177 95 9 116 276 165 335 1.07e-14 75.1
Msa0131610 MtrunA17_Chr5g0435721 28.019 207 123 7 69 262 224 417 3.82e-14 73.9
Msa0131610 MtrunA17_Chr7g0221691 36.145 166 85 8 131 283 231 388 1.29e-13 72.4
Msa0131610 MtrunA17_Chr3g0138281 27.969 261 145 9 67 308 120 356 2.16e-13 71.2
Msa0131610 MtrunA17_Chr6g0486661 27.517 298 167 14 36 297 97 381 3.06e-13 72.0
Msa0131610 MtrunA17_Chr4g0066371 31.126 302 148 19 36 297 59 340 3.83e-13 70.5
Msa0131610 MtrunA17_Chr5g0394181 27.907 258 140 11 66 297 360 597 9.94e-13 70.1
Msa0131610 MtrunA17_Chr2g0286951 27.431 288 163 12 43 297 67 341 3.19e-12 67.8
Msa0131610 MtrunA17_Chr4g0001761 34.043 141 66 5 128 257 156 280 3.62e-12 67.4
Msa0131610 MtrunA17_Chr8g0342431 30.189 159 94 7 136 283 201 353 3.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0131610 AT1G09160.2 75.118 422 103 1 1 422 1 420 0.0 645
Msa0131610 AT1G09160.1 75.118 422 103 1 1 422 1 420 0.0 645
Msa0131610 AT1G68410.4 68.434 415 127 2 11 422 16 429 0.0 578
Msa0131610 AT1G68410.3 68.434 415 127 2 11 422 16 429 0.0 578
Msa0131610 AT1G68410.1 68.434 415 127 2 11 422 16 429 0.0 578
Msa0131610 AT1G68410.2 68.434 415 127 2 11 422 16 429 0.0 578
Msa0131610 AT1G47380.1 56.341 410 178 1 14 423 10 418 7.69e-170 483
Msa0131610 AT5G10740.1 30.921 304 168 13 6 298 8 280 5.41e-27 111
Msa0131610 AT5G10740.2 30.921 304 168 13 6 298 8 280 5.41e-27 111
Msa0131610 AT2G34740.1 33.054 239 136 8 65 298 116 335 7.79e-27 110
Msa0131610 AT1G67820.2 32.787 244 139 10 34 270 78 303 6.64e-26 108
Msa0131610 AT1G67820.1 32.787 244 139 10 34 270 114 339 8.88e-26 108
Msa0131610 AT5G24940.1 30.877 285 154 13 25 298 28 280 1.49e-25 108
Msa0131610 AT4G31750.1 29.180 305 163 13 25 306 28 302 2.62e-25 105
Msa0131610 AT4G31750.3 29.180 305 163 13 25 306 28 302 2.62e-25 105
Msa0131610 AT4G31750.2 30.108 279 158 11 25 295 28 277 3.08e-25 105
Msa0131610 AT1G07160.1 30.325 277 158 11 33 297 123 376 4.66e-25 106
Msa0131610 AT2G40180.1 28.622 283 182 8 21 297 117 385 2.39e-24 104
Msa0131610 AT1G43900.1 31.970 269 148 14 36 295 124 366 3.52e-24 103
Msa0131610 AT1G22280.3 30.901 233 143 7 68 295 65 284 3.71e-24 101
Msa0131610 AT1G34750.2 29.749 279 161 11 27 295 26 279 1.74e-23 99.8
Msa0131610 AT1G34750.1 29.749 279 161 11 27 295 26 279 1.74e-23 99.8
Msa0131610 AT1G34750.3 29.749 279 161 11 27 295 26 279 1.74e-23 99.8
Msa0131610 AT1G78200.5 31.128 257 147 10 46 295 2 235 1.97e-23 98.6
Msa0131610 AT1G78200.4 31.128 257 147 10 46 295 2 235 1.97e-23 98.6
Msa0131610 AT1G78200.1 30.357 280 161 11 27 295 24 280 2.06e-23 99.8
Msa0131610 AT1G78200.3 30.357 280 161 11 27 295 24 280 2.06e-23 99.8
Msa0131610 AT1G78200.2 30.357 280 161 11 27 295 24 280 2.06e-23 99.8
Msa0131610 AT2G30020.1 28.782 271 163 11 36 297 143 392 7.17e-23 100
Msa0131610 AT4G28400.3 29.258 229 146 6 68 295 45 258 8.29e-23 97.4
Msa0131610 AT5G53140.1 29.412 255 151 10 46 295 115 345 1.37e-22 99.8
Msa0131610 AT5G53140.2 29.412 255 151 10 46 295 115 345 1.37e-22 99.8
Msa0131610 AT4G28400.1 29.258 229 146 6 68 295 67 280 1.50e-22 97.4
Msa0131610 AT1G22280.1 30.901 233 137 8 68 295 65 278 3.90e-22 95.9
Msa0131610 AT2G20630.1 29.104 268 158 12 33 295 36 276 5.52e-22 95.5
Msa0131610 AT4G26080.1 27.737 274 149 7 61 299 165 424 9.52e-22 97.4
Msa0131610 AT2G20630.2 28.839 267 158 12 33 294 36 275 2.11e-21 94.0
Msa0131610 AT3G51470.2 28.475 295 169 11 36 314 32 300 5.98e-21 93.2
Msa0131610 AT3G51470.1 28.475 295 169 11 36 314 76 344 8.05e-21 93.6
Msa0131610 AT5G57050.2 28.464 267 145 9 65 297 117 371 3.40e-20 92.0
Msa0131610 AT5G57050.1 28.464 267 145 9 65 297 157 411 4.33e-20 92.4
Msa0131610 AT2G40860.1 29.286 280 157 9 36 299 396 650 1.16e-19 92.0
Msa0131610 AT2G40860.4 29.286 280 157 9 36 299 298 552 1.71e-19 91.3
Msa0131610 AT2G25620.1 30.451 266 136 10 67 308 127 367 1.87e-19 90.1
Msa0131610 AT2G25620.2 30.451 266 136 10 67 308 127 367 1.87e-19 90.1
Msa0131610 AT2G34740.3 29.665 209 127 5 65 270 112 303 2.68e-19 88.6
Msa0131610 AT2G34740.2 29.665 209 127 5 65 270 130 321 3.29e-19 88.6
Msa0131610 AT3G62260.3 29.771 262 148 12 67 307 26 272 3.29e-18 85.1
Msa0131610 AT2G40860.2 28.409 264 148 8 36 282 396 635 8.87e-18 86.3
Msa0131610 AT3G62260.1 29.771 262 148 12 67 307 115 361 1.14e-17 84.7
Msa0131610 AT1G48040.1 32.692 260 129 12 63 295 107 347 1.16e-17 84.7
Msa0131610 AT3G62260.2 29.771 262 148 12 67 307 116 362 1.22e-17 84.7
Msa0131610 AT3G15260.2 29.185 233 141 8 68 295 73 286 6.40e-17 81.3
Msa0131610 AT3G15260.1 29.185 233 141 8 68 295 73 286 6.40e-17 81.3
Msa0131610 AT1G34750.4 28.085 235 136 9 27 252 26 236 1.30e-16 79.3
Msa0131610 AT5G59220.1 28.287 251 150 10 31 265 107 343 3.80e-15 77.4
Msa0131610 AT4G28400.2 27.568 185 120 4 68 252 67 237 4.23e-15 75.1
Msa0131610 AT1G07430.1 29.148 223 121 8 62 265 151 355 3.92e-14 74.3
Msa0131610 AT4G27800.1 25.378 331 170 13 40 322 67 368 3.14e-13 71.2
Msa0131610 AT2G20050.2 28.053 303 156 16 36 297 116 397 5.21e-13 71.6
Msa0131610 AT2G20050.1 27.393 303 158 15 36 297 116 397 5.50e-13 71.6
Msa0131610 AT3G11410.1 25.446 224 126 7 62 260 131 338 1.18e-12 69.7
Msa0131610 AT3G17250.1 28.063 253 140 13 67 295 157 391 1.91e-12 68.9
Msa0131610 AT2G40860.3 29.114 237 127 8 36 255 396 608 2.49e-12 69.3
Msa0131610 AT1G16220.1 23.864 352 195 13 5 301 45 378 2.67e-12 68.9
Msa0131610 AT1G17550.1 27.434 226 120 8 69 265 238 448 8.95e-12 67.4
Msa0131610 AT1G17550.2 27.434 226 120 8 69 265 238 448 8.95e-12 67.4
Msa0131610 AT5G27930.3 33.544 158 88 7 105 251 150 301 9.21e-12 66.2
Msa0131610 AT4G27800.3 26.766 269 131 10 40 265 67 312 1.27e-11 65.9
Msa0131610 AT4G27800.2 26.766 269 131 10 40 265 67 312 1.44e-11 65.9
Msa0131610 AT1G79630.2 29.798 198 121 6 131 314 151 344 1.55e-11 66.2
Msa0131610 AT1G79630.7 29.798 198 121 6 131 314 151 344 1.55e-11 66.2
Msa0131610 AT1G79630.6 29.500 200 123 6 131 316 111 306 1.78e-11 65.9
Msa0131610 AT1G79630.3 29.500 200 123 6 131 316 111 306 1.78e-11 65.9
Msa0131610 AT1G79630.4 29.500 200 123 6 131 316 111 306 1.78e-11 65.9
Msa0131610 AT1G79630.5 29.500 200 123 6 131 316 111 306 1.78e-11 65.9
Msa0131610 AT1G79630.8 29.500 200 123 6 131 316 217 412 1.92e-11 66.2
Msa0131610 AT1G79630.1 29.500 200 123 6 131 316 221 416 2.96e-11 65.5
Msa0131610 AT5G36250.1 26.222 225 136 9 100 300 171 389 4.86e-11 64.7
Msa0131610 AT4G32950.1 30.114 176 110 5 131 297 138 309 5.03e-11 63.9
Msa0131610 AT2G29380.1 27.928 222 132 8 59 265 103 311 6.79e-11 63.9
Msa0131610 AT1G72770.4 24.727 275 149 9 69 297 239 501 7.06e-11 64.3
Msa0131610 AT1G72770.1 24.727 275 149 9 69 297 239 501 7.06e-11 64.3
Msa0131610 AT1G72770.3 24.727 275 149 9 69 297 239 501 7.06e-11 64.3

Find 137 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGCTTCTGATGGAATT+TGG 0.123861 1_3:-62485172 Msa0131610:CDS
AGCACATTATGTTTCTTTCT+TGG 0.197447 1_3:+62484878 None:intergenic
GTTCCTTCCAATACAAATTC+TGG 0.255485 1_3:-62484266 Msa0131610:CDS
TGTTTCTTTCTTGGAGTTGC+TGG 0.268275 1_3:+62484887 None:intergenic
CTGTGCTCCCAACACCCATT+AGG 0.273813 1_3:-62487068 Msa0131610:five_prime_UTR
GTGCTTAGTTCACTTCTCTT+TGG 0.288508 1_3:-62484860 Msa0131610:CDS
GCAATGCTTGGTTCTACATT+TGG 0.316695 1_3:-62483995 Msa0131610:three_prime_UTR
TCACCTGCAGGGGAAACTTC+TGG 0.319705 1_3:-62485717 Msa0131610:intron
TATAGCTTCTGATGGAATTT+GGG 0.324404 1_3:-62485171 Msa0131610:CDS
ACTGTTGTTGCTCGGACTTC+AGG 0.329448 1_3:+62484085 None:intergenic
GCTACGTTTGTGATTATTGA+TGG 0.331297 1_3:-62485687 Msa0131610:CDS
TGTGCTCCCAACACCCATTA+GGG 0.343384 1_3:-62487067 Msa0131610:five_prime_UTR
CCGTTACAGTTGCATCTGTT+GGG 0.351128 1_3:-62485659 Msa0131610:CDS
CAATGCTTGGTTCTACATTT+GGG 0.352020 1_3:-62483994 Msa0131610:three_prime_UTR
ACAGATTGCCATAGAGTTCC+TGG 0.368715 1_3:-62486337 Msa0131610:CDS
ACTTGCTTAACATGTGGTAT+TGG 0.372029 1_3:+62485328 None:intergenic
TGCTGCCAAGCTGGTGGTTA+AGG 0.391611 1_3:-62485093 Msa0131610:intron
TAATTTGCAACAACCTTCAA+TGG 0.406917 1_3:+62483908 None:intergenic
TATATTGATTTGATTGATAA+CGG 0.408391 1_3:-62483743 Msa0131610:three_prime_UTR
AAGACGCCATGGAAGGAAAA+AGG 0.416517 1_3:-62484109 Msa0131610:CDS
CAGATCCTTGACGGGCATAA+TGG 0.417208 1_3:-62485965 Msa0131610:CDS
GGGCCTCTTCGCTGTTGGCC+TGG 0.420476 1_3:-62485406 Msa0131610:CDS
AACCTGAACAGGAAGCTCTA+AGG 0.420809 1_3:-62484985 Msa0131610:intron
TTTGGCTTGTGCTCAGCTGT+TGG 0.425707 1_3:-62483882 Msa0131610:three_prime_UTR
GTAGGAAGACTCAACGTATT+CGG 0.426631 1_3:-62485522 Msa0131610:CDS
GTTTCCTAGCTTTCAAATGC+TGG 0.427993 1_3:-62485206 Msa0131610:intron
CTTGATTGGCAGATCCTTGA+CGG 0.435403 1_3:-62485974 Msa0131610:intron
TGGAAGAAAATGTAGAAGAG+AGG 0.437691 1_3:-62485572 Msa0131610:CDS
GTGAATACCAGAATTTGTAT+TGG 0.439513 1_3:+62484259 None:intergenic
CTTAAAATGCAGGCTAGGTA+AGG 0.443855 1_3:-62484291 Msa0131610:intron
ATGGCTTCAAGCACTGCCTC+GGG 0.448779 1_3:-62485861 Msa0131610:CDS
ACTAATAAGCTTTCTGCAGT+TGG 0.455037 1_3:-62484806 Msa0131610:CDS
TCGTAGCTCACGGCCGATCA+AGG 0.456909 1_3:+62486441 None:intergenic
CCATACCGCAAGCTGTAAGC+AGG 0.459728 1_3:-62485889 Msa0131610:CDS
GGATACTTTGTCTTCTGATA+CGG 0.461894 1_3:-62485150 Msa0131610:CDS
AATGGCTTCAAGCACTGCCT+CGG 0.462446 1_3:-62485862 Msa0131610:CDS
ATCGGCCGTGAGCTACGAAA+TGG 0.463670 1_3:-62486436 Msa0131610:CDS
GTTCCGTTGGCAACCTTGAT+CGG 0.465033 1_3:-62486454 Msa0131610:CDS
ACAATTGAGTTAGCTAGGTT+AGG 0.469351 1_3:-62483932 Msa0131610:three_prime_UTR
AAGAATCGCCAGGAACTCTA+TGG 0.471757 1_3:+62486329 None:intergenic
GCCTGTCCATACTTCACAAA+AGG 0.476504 1_3:+62486400 None:intergenic
TTTCTTGGAGTTGCTGGCAT+CGG 0.478380 1_3:+62484893 None:intergenic
GAAGTATGGACAGGCTGGTT+TGG 0.479502 1_3:-62486392 Msa0131610:CDS
GTGGAGGAGTTATTTGAAGA+GGG 0.481037 1_3:-62484779 Msa0131610:CDS
CTGGGTATCTAATATGCATC+GGG 0.481436 1_3:+62485632 None:intergenic
ACCGTTACAGTTGCATCTGT+TGG 0.483048 1_3:-62485660 Msa0131610:CDS
TGCAGAACTTGCTGCCAAGC+TGG 0.486136 1_3:-62485102 Msa0131610:CDS
GGTTGACAATTGAGTTAGCT+AGG 0.487191 1_3:-62483937 Msa0131610:three_prime_UTR
TCTTCCTACTTCACCACCAC+TGG 0.489968 1_3:+62485536 None:intergenic
AATAATATCTACAACAAGGC+AGG 0.494608 1_3:+62484937 None:intergenic
GCTTTCTGCAGTTGGTATTG+TGG 0.496447 1_3:-62484798 Msa0131610:CDS
AGCTAGGTTAGGTCCATTGA+AGG 0.499451 1_3:-62483921 Msa0131610:three_prime_UTR
TGACAGTTTGTGCAGAGGAA+TGG 0.499633 1_3:+62484140 None:intergenic
CCTTCCCATGGCTTAGATAC+CGG 0.499863 1_3:+62484164 None:intergenic
TGCCTAGCAGATCAACCATC+TGG 0.501426 1_3:-62484224 Msa0131610:CDS
TTTGTGAAGTATGGACAGGC+TGG 0.503204 1_3:-62486397 Msa0131610:CDS
AAAATGTAGAAGAGAGGGAG+CGG 0.505425 1_3:-62485566 Msa0131610:CDS
CCTGCTTACAGCTTGCGGTA+TGG 0.506291 1_3:+62485889 None:intergenic
CGTTTGTGATTATTGATGGA+TGG 0.510062 1_3:-62485683 Msa0131610:CDS
AGAGAGAAACAACACCTCCC+TGG 0.510635 1_3:+62485613 None:intergenic
TCCTCCCTGCTTACAGCTTG+CGG 0.511139 1_3:+62485884 None:intergenic
TTGGCAGTGTGAGCAATGCT+TGG 0.513571 1_3:-62484007 Msa0131610:three_prime_UTR
TTCTGCAGTTGGTATTGTGG+AGG 0.515246 1_3:-62484795 Msa0131610:CDS
AGCTTGGCAGCAAGTTCTGC+AGG 0.516289 1_3:+62485104 None:intergenic
AGGTAGGGCCTCTTCGCTGT+TGG 0.519693 1_3:-62485411 Msa0131610:CDS
ACAGCCAGTGGTGGTGAAGT+AGG 0.520024 1_3:-62485540 Msa0131610:CDS
CGTTACAGTTGCATCTGTTG+GGG 0.531574 1_3:-62485658 Msa0131610:CDS
TTGATTGGCAGATCCTTGAC+GGG 0.533037 1_3:-62485973 Msa0131610:intron
GAAGCAACCACTTGTTCCGT+TGG 0.533409 1_3:-62486467 Msa0131610:CDS
TGAGTAAGACTGAATTATCA+AGG 0.534598 1_3:-62486492 Msa0131610:CDS
ATAAAATGATCGGTGTTCAC+TGG 0.535304 1_3:+62484191 None:intergenic
CCTCTTCGCTGTTGGCCTGG+TGG 0.536837 1_3:-62485403 Msa0131610:CDS
AAATGTAGAAGAGAGGGAGC+GGG 0.539423 1_3:-62485565 Msa0131610:CDS
TGTGGAGGAGTTATTTGAAG+AGG 0.540145 1_3:-62484780 Msa0131610:CDS
AATGACGTTGAAGAATCGCC+AGG 0.544088 1_3:+62486319 None:intergenic
CCTGGGTATCTAATATGCAT+CGG 0.544206 1_3:+62485631 None:intergenic
ATACCAGAATTTGTATTGGA+AGG 0.544653 1_3:+62484263 None:intergenic
TCACTCCGGTATCTAAGCCA+TGG 0.550001 1_3:-62484169 Msa0131610:CDS
AGACTAATTATAGCTTCTGA+TGG 0.551958 1_3:-62485179 Msa0131610:CDS
TCTAAGGTCAAGAGGGCTGA+AGG 0.552829 1_3:-62484969 Msa0131610:CDS
TGTCACCAATCGATCTAGAA+AGG 0.554127 1_3:+62485374 None:intergenic
GAGAGAAACAACACCTCCCT+GGG 0.554466 1_3:+62485614 None:intergenic
AGGGAGCGGGTTACAGCCAG+TGG 0.559334 1_3:-62485552 Msa0131610:CDS
GGAAGAAAATGTAGAAGAGA+GGG 0.560448 1_3:-62485571 Msa0131610:CDS
CACTCCGGTATCTAAGCCAT+GGG 0.564768 1_3:-62484168 Msa0131610:CDS
CTAGAAAGGCACAATCCACC+AGG 0.566834 1_3:+62485388 None:intergenic
CCGATGCATATTAGATACCC+AGG 0.567386 1_3:-62485631 Msa0131610:CDS
AAGCTGTAAGCAGGGAGGAA+TGG 0.567687 1_3:-62485880 Msa0131610:CDS
TCTTCTGACAGTTTGTGCAG+AGG 0.570739 1_3:+62484135 None:intergenic
TTGTGCCTTTCTAGATCGAT+TGG 0.574242 1_3:-62485379 Msa0131610:CDS
CACCAGATGGTTGATCTGCT+AGG 0.577655 1_3:+62484222 None:intergenic
AGGTACACCACGACATGACT+TGG 0.578313 1_3:+62485124 None:intergenic
CAGGAAGCTCTAAGGTCAAG+AGG 0.580501 1_3:-62484977 Msa0131610:CDS
GTTGCTGGCATCGGCAACAC+AGG 0.580752 1_3:+62484902 None:intergenic
ACCTTCACTTGCTTAACATG+TGG 0.582513 1_3:+62485322 None:intergenic
CAGAGGAATGGACCTTCCCA+TGG 0.585428 1_3:+62484152 None:intergenic
TCTCAAAACTCGACAAACAA+AGG 0.587947 1_3:-62484830 Msa0131610:CDS
TGCTTGGTTCTACATTTGGG+TGG 0.590730 1_3:-62483991 Msa0131610:three_prime_UTR
ATAACTATACTGTTGTTGCT+CGG 0.593054 1_3:+62484077 None:intergenic
CTTGACAGTTGATCACAGAC+TGG 0.593422 1_3:-62485592 Msa0131610:CDS
AAGAAGAAAGACGCCATGGA+AGG 0.594109 1_3:-62484116 Msa0131610:CDS
ATCGATTGGTGACACCGATG+TGG 0.601496 1_3:-62485365 Msa0131610:CDS
GCAGATCAACCATCTGGTGA+TGG 0.602774 1_3:-62484218 Msa0131610:CDS
AAGCATTGCTCACACTGCCA+AGG 0.610583 1_3:+62484009 None:intergenic
TACCGGAGTGATAAAATGAT+CGG 0.613081 1_3:+62484181 None:intergenic
CATCACCTTAACCACCAGCT+TGG 0.613564 1_3:+62485088 None:intergenic
GAAATGTTGACTTATAAACT+AGG 0.614349 1_3:-62486516 Msa0131610:five_prime_UTR
CCACCAGGCCAACAGCGAAG+AGG 0.616089 1_3:+62485403 None:intergenic
TCGATTGGTGACACCGATGT+GGG 0.617709 1_3:-62485364 Msa0131610:CDS
AAGGTTGCCAACGGAACAAG+TGG 0.618923 1_3:+62486460 None:intergenic
ATATACCATTATGCCCGTCA+AGG 0.625718 1_3:+62485960 None:intergenic
GAGCGGGTTACAGCCAGTGG+TGG 0.626341 1_3:-62485549 Msa0131610:CDS
AGGAAGCTCTAAGGTCAAGA+GGG 0.627250 1_3:-62484976 Msa0131610:CDS
ACCACATGTTAAGCAAGTGA+AGG 0.627901 1_3:-62485323 Msa0131610:intron
TCAGAAGAAGAAAGACGCCA+TGG 0.630309 1_3:-62484120 Msa0131610:CDS
CCCAACAGATGCAACTGTAA+CGG 0.630410 1_3:+62485659 None:intergenic
CGGCCGATCAAGGTTGCCAA+CGG 0.631386 1_3:+62486451 None:intergenic
TCCTAGCTTTCAAATGCTGG+CGG 0.631854 1_3:-62485203 Msa0131610:intron
CCGGTATCTAAGCCATGGGA+AGG 0.632107 1_3:-62484164 Msa0131610:CDS
TACAGAAATGTTGTATACAG+AGG 0.632924 1_3:+62487129 None:intergenic
GGCAACACAGGATAGTCTGA+AGG 0.635849 1_3:+62484914 None:intergenic
AAAGCGACAACTAGAGCCCG+AGG 0.640273 1_3:+62485845 None:intergenic
CAACGTATTCGGAGGCAATG+AGG 0.642352 1_3:-62485511 Msa0131610:intron
CGATGCATATTAGATACCCA+GGG 0.643134 1_3:-62485630 Msa0131610:CDS
ACTGGTAAACCATCACCAGA+TGG 0.656343 1_3:+62484209 None:intergenic
CATACCGCAAGCTGTAAGCA+GGG 0.656614 1_3:-62485888 Msa0131610:CDS
AAGTGTGTGTGTGACAAATG+AGG 0.666861 1_3:-62483958 Msa0131610:three_prime_UTR
GGAAGACTCAACGTATTCGG+AGG 0.678380 1_3:-62485519 Msa0131610:CDS
AACGATATACTCTCCCACAT+CGG 0.681964 1_3:+62485351 None:intergenic
AGAACTTGCTGCCAAGCTGG+TGG 0.683075 1_3:-62485099 Msa0131610:CDS
ACGGCTGCCAAGTCATGTCG+TGG 0.691102 1_3:-62485131 Msa0131610:CDS
TGTTGTATACAGAGGAAACG+CGG 0.694406 1_3:+62487137 None:intergenic
AAGGAATAATATCTACAACA+AGG 0.708500 1_3:+62484933 None:intergenic
ACCGCAAGCTGTAAGCAGGG+AGG 0.738061 1_3:-62485885 Msa0131610:CDS
GTTCTGCAATGCTTACAGAG+AGG 0.751165 1_3:-62484757 Msa0131610:intron
TGCATATTAGATACCCAGGG+AGG 0.753250 1_3:-62485627 Msa0131610:CDS
CTGCTAGGCAAACTGCACAG+CGG 0.759343 1_3:+62484237 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAATAATTGTTTTTTA+AGG + chr1_3:62483760-62483779 None:intergenic 10.0%
!! ATAAAAAGATAAATAAAATC+TGG + chr1_3:62483997-62484016 None:intergenic 10.0%
!!! ATAAATATATTGATTTTGTT+TGG - chr1_3:62484381-62484400 Msa0131610:intron 10.0%
!!! TTGAAAATGCATTTTATAAA+TGG - chr1_3:62486387-62486406 Msa0131610:CDS 15.0%
!!! TCAATTTTTACTTTTTACAT+CGG + chr1_3:62486549-62486568 None:intergenic 15.0%
!!! ATATTTGTATAATTTTTCCT+TGG - chr1_3:62486912-62486931 Msa0131610:intron 15.0%
!!! TATATTGATTTGATTGATAA+CGG - chr1_3:62487195-62487214 Msa0131610:five_prime_UTR 15.0%
!!! CTATGATTAATTTGATTCTA+TGG - chr1_3:62484197-62484216 Msa0131610:CDS 20.0%
!! ATAAGATCTTATGAATGAAT+TGG - chr1_3:62484779-62484798 Msa0131610:CDS 20.0%
!! TAAGGAAGAAATTATATGAA+AGG + chr1_3:62485478-62485497 None:intergenic 20.0%
!!! TTTTGGTTCCTTAATATTTT+AGG - chr1_3:62485507-62485526 Msa0131610:intron 20.0%
!! AAGAACTACTAAACTTTAAA+AGG + chr1_3:62486249-62486268 None:intergenic 20.0%
!!! CATTTGATTATGAATCTTTT+TGG + chr1_3:62486514-62486533 None:intergenic 20.0%
!!! TTTTTGTTTTCTTAAAATGC+AGG - chr1_3:62486637-62486656 Msa0131610:intron 20.0%
!!! TTAACATTACACTTTTTGTT+TGG - chr1_3:62487164-62487183 Msa0131610:five_prime_UTR 20.0%
! AAATGAAGAAGAAAAACTCA+CGG + chr1_3:62483958-62483977 None:intergenic 25.0%
!! AGAATGTTGATTATTATAGC+TGG + chr1_3:62484142-62484161 None:intergenic 25.0%
! AATTTGATTCTATGGAAGTA+AGG - chr1_3:62484205-62484224 Msa0131610:CDS 25.0%
!! TAGATCTATTAAGAGTCTTT+TGG - chr1_3:62484338-62484357 Msa0131610:intron 25.0%
!!! TTGATTTTGTTTGGATGATT+AGG - chr1_3:62484390-62484409 Msa0131610:intron 25.0%
!!! GAAATGTTGACTTATAAACT+AGG - chr1_3:62484422-62484441 Msa0131610:intron 25.0%
! ATTGATATCATAACCATTGT+GGG + chr1_3:62484936-62484955 None:intergenic 25.0%
! TATTGATATCATAACCATTG+TGG + chr1_3:62484937-62484956 None:intergenic 25.0%
! CAATGGTTATGATATCAATA+AGG - chr1_3:62484937-62484956 Msa0131610:CDS 25.0%
! ATCAATAAGGAATTCTTGAT+TGG - chr1_3:62484950-62484969 Msa0131610:CDS 25.0%
!! AGTTTTCTTTGTATCCTTAT+TGG - chr1_3:62485158-62485177 Msa0131610:CDS 25.0%
!! TAAAATTTAATTTCACCTGC+AGG - chr1_3:62485209-62485228 Msa0131610:intron 25.0%
!! AAAATTTAATTTCACCTGCA+GGG - chr1_3:62485210-62485229 Msa0131610:intron 25.0%
!!! GTTCCTTAATATTTTAGGTA+GGG - chr1_3:62485512-62485531 Msa0131610:CDS 25.0%
! AAGGAATAATATCTACAACA+AGG + chr1_3:62486008-62486027 None:intergenic 25.0%
! TGATTGGAACAAAAATGTTT+AGG - chr1_3:62486288-62486307 Msa0131610:intron 25.0%
! GATTGGAACAAAAATGTTTA+GGG - chr1_3:62486289-62486308 Msa0131610:intron 25.0%
!!! GGTTGTTGCAAATTATTTTT+TGG - chr1_3:62487038-62487057 Msa0131610:five_prime_UTR 25.0%
TACAGAAATGTTGTATACAG+AGG + chr1_3:62483812-62483831 None:intergenic 30.0%
AAGAGTTGAAAAACCCTAAT+GGG + chr1_3:62483887-62483906 None:intergenic 30.0%
!! CAGAATTTTGAGTATGAGAA+AGG + chr1_3:62483912-62483931 None:intergenic 30.0%
AATGAAGAAGAAAAACTCAC+GGG + chr1_3:62483957-62483976 None:intergenic 30.0%
AGTATCTTAGCTTAGTTAGT+TGG - chr1_3:62484065-62484084 Msa0131610:three_prime_UTR 30.0%
! TTTTGTACTTCTAGACTCAA+TGG - chr1_3:62484269-62484288 Msa0131610:CDS 30.0%
TGAGTAAGACTGAATTATCA+AGG - chr1_3:62484446-62484465 Msa0131610:intron 30.0%
TTAGTGAAATGTTTGATGAG+AGG - chr1_3:62484721-62484740 Msa0131610:intron 30.0%
!! TTTGATGAGAGGTATTTGAT+TGG - chr1_3:62484732-62484751 Msa0131610:intron 30.0%
TAAGAAAGATACATTCCCTT+TGG + chr1_3:62484827-62484846 None:intergenic 30.0%
AAAGGGAATGTATCTTTCTT+AGG - chr1_3:62484826-62484845 Msa0131610:CDS 30.0%
GACATGGAATTTCAGAAAAA+AGG - chr1_3:62485126-62485145 Msa0131610:CDS 30.0%
! AAATTTAATTTCACCTGCAG+GGG - chr1_3:62485211-62485230 Msa0131610:intron 30.0%
!!! CTTCCTTATCTAACTTGTTT+TGG - chr1_3:62485490-62485509 Msa0131610:intron 30.0%
GAACCAAAACAAGTTAGATA+AGG + chr1_3:62485496-62485515 None:intergenic 30.0%
!! GGTTCCTTAATATTTTAGGT+AGG - chr1_3:62485511-62485530 Msa0131610:intron 30.0%
GTAAACACCTTCACATAAAA+GGG + chr1_3:62485677-62485696 None:intergenic 30.0%
AGTAAACACCTTCACATAAA+AGG + chr1_3:62485678-62485697 None:intergenic 30.0%
AGACTAATTATAGCTTCTGA+TGG - chr1_3:62485759-62485778 Msa0131610:intron 30.0%
TTATAGCTTCTGATGGAATT+TGG - chr1_3:62485766-62485785 Msa0131610:intron 30.0%
TATAGCTTCTGATGGAATTT+GGG - chr1_3:62485767-62485786 Msa0131610:intron 30.0%
GAATTCAAGTCTCATTATGT+TGG - chr1_3:62485904-62485923 Msa0131610:CDS 30.0%
!!! TCTATTGTTTTAACCTGAAC+AGG - chr1_3:62485942-62485961 Msa0131610:CDS 30.0%
AATAATATCTACAACAAGGC+AGG + chr1_3:62486004-62486023 None:intergenic 30.0%
AGCACATTATGTTTCTTTCT+TGG + chr1_3:62486063-62486082 None:intergenic 30.0%
!! AACTAGAGAAAGTGCATTTT+CGG + chr1_3:62486466-62486485 None:intergenic 30.0%
!!! ATGTCCTTATTTTCCCATTT+TGG - chr1_3:62486590-62486609 Msa0131610:intron 30.0%
!!! TGTCCTTATTTTCCCATTTT+GGG - chr1_3:62486591-62486610 Msa0131610:intron 30.0%
ATACCAGAATTTGTATTGGA+AGG + chr1_3:62486678-62486697 None:intergenic 30.0%
GTGAATACCAGAATTTGTAT+TGG + chr1_3:62486682-62486701 None:intergenic 30.0%
ATAACTATACTGTTGTTGCT+CGG + chr1_3:62486864-62486883 None:intergenic 30.0%
! TAATTTGCAACAACCTTCAA+TGG + chr1_3:62487033-62487052 None:intergenic 30.0%
! TTTGCTCTCTGATTTTCGAT+TGG + chr1_3:62483835-62483854 None:intergenic 35.0%
GAAGAGTTGAAAAACCCTAA+TGG + chr1_3:62483888-62483907 None:intergenic 35.0%
ATGAGCACTATAGTGAAACA+TGG + chr1_3:62484236-62484255 None:intergenic 35.0%
TCTACACATGTTGAGTGAAT+TGG + chr1_3:62484322-62484341 None:intergenic 35.0%
! TTTTTCCATTTCGTAGCTCA+CGG + chr1_3:62484510-62484529 None:intergenic 35.0%
TGAGCTACGAAATGGAAAAA+CGG - chr1_3:62484510-62484529 Msa0131610:intron 35.0%
!! AACGTCATTTTCTGTTTTCG+CGG - chr1_3:62484633-62484652 Msa0131610:intron 35.0%
! ACATTTTCTCAGTGATCAAG+TGG - chr1_3:62484896-62484915 Msa0131610:CDS 35.0%
GCTACGTTTGTGATTATTGA+TGG - chr1_3:62485251-62485270 Msa0131610:intron 35.0%
CGTTTGTGATTATTGATGGA+TGG - chr1_3:62485255-62485274 Msa0131610:intron 35.0%
TGGAAGAAAATGTAGAAGAG+AGG - chr1_3:62485366-62485385 Msa0131610:CDS 35.0%
GGAAGAAAATGTAGAAGAGA+GGG - chr1_3:62485367-62485386 Msa0131610:CDS 35.0%
AGGCCCTACCTAAAATATTA+AGG + chr1_3:62485518-62485537 None:intergenic 35.0%
ACTTGCTTAACATGTGGTAT+TGG + chr1_3:62485613-62485632 None:intergenic 35.0%
!!! GTGAGTTGGTTTCATTTTCA+TGG - chr1_3:62485637-62485656 Msa0131610:CDS 35.0%
!! TTCTAAAGTTCAATGCTCAC+TGG - chr1_3:62485697-62485716 Msa0131610:CDS 35.0%
AAAGCTAGGAAACAAATCAG+GGG + chr1_3:62485725-62485744 None:intergenic 35.0%
GAAAGCTAGGAAACAAATCA+GGG + chr1_3:62485726-62485745 None:intergenic 35.0%
TGAAAGCTAGGAAACAAATC+AGG + chr1_3:62485727-62485746 None:intergenic 35.0%
GGATACTTTGTCTTCTGATA+CGG - chr1_3:62485788-62485807 Msa0131610:intron 35.0%
! GTTAAGGTGATGGTTATTTG+TGG - chr1_3:62485861-62485880 Msa0131610:CDS 35.0%
!!! GGCAGTGTTCTTATTTTTTG+AGG - chr1_3:62485882-62485901 Msa0131610:CDS 35.0%
TCTCAAAACTCGACAAACAA+AGG - chr1_3:62486108-62486127 Msa0131610:intron 35.0%
ACTAATAAGCTTTCTGCAGT+TGG - chr1_3:62486132-62486151 Msa0131610:intron 35.0%
! TTCCAATCACTTGTTGATGA+TGG + chr1_3:62486277-62486296 None:intergenic 35.0%
! TGTTTAGGGTCTGTTTACTT+CGG - chr1_3:62486303-62486322 Msa0131610:intron 35.0%
ACACCCAAAATGGGAAAATA+AGG + chr1_3:62486597-62486616 None:intergenic 35.0%
CACATGAATACACCCAAAAT+GGG + chr1_3:62486606-62486625 None:intergenic 35.0%
ACACATGAATACACCCAAAA+TGG + chr1_3:62486607-62486626 None:intergenic 35.0%
! GTTTTCTTAAAATGCAGGCT+AGG - chr1_3:62486642-62486661 Msa0131610:intron 35.0%
GTTCCTTCCAATACAAATTC+TGG - chr1_3:62486672-62486691 Msa0131610:intron 35.0%
! ATAAAATGATCGGTGTTCAC+TGG + chr1_3:62486750-62486769 None:intergenic 35.0%
TACCGGAGTGATAAAATGAT+CGG + chr1_3:62486760-62486779 None:intergenic 35.0%
!! CAATGCTTGGTTCTACATTT+GGG - chr1_3:62486944-62486963 Msa0131610:intron 35.0%
ACAATTGAGTTAGCTAGGTT+AGG - chr1_3:62487006-62487025 Msa0131610:five_prime_UTR 35.0%
TGTTGTATACAGAGGAAACG+CGG + chr1_3:62483804-62483823 None:intergenic 40.0%
! GAAAAACCCTAATGGGTGTT+GGG + chr1_3:62483880-62483899 None:intergenic 40.0%
! TGAAAAACCCTAATGGGTGT+TGG + chr1_3:62483881-62483900 None:intergenic 40.0%
! GAGAAGCCTTTTGTGAAGTA+TGG - chr1_3:62484532-62484551 Msa0131610:intron 40.0%
! TCATTTTCTGTTTTCGCGGT+AGG - chr1_3:62484637-62484656 Msa0131610:intron 40.0%
GCGTCAATTTATTCCCACAA+TGG - chr1_3:62484920-62484939 Msa0131610:CDS 40.0%
ATATACCATTATGCCCGTCA+AGG + chr1_3:62484981-62485000 None:intergenic 40.0%
TTCCTTCGCAAAAATAGCAG+CGG + chr1_3:62485004-62485023 None:intergenic 40.0%
! CGCTTTTGTGAAAACTGACA+TGG - chr1_3:62485110-62485129 Msa0131610:CDS 40.0%
GAACAAGTACAGCTCCAATA+AGG + chr1_3:62485175-62485194 None:intergenic 40.0%
CTGGGTATCTAATATGCATC+GGG + chr1_3:62485309-62485328 None:intergenic 40.0%
CCTGGGTATCTAATATGCAT+CGG + chr1_3:62485310-62485329 None:intergenic 40.0%
CGATGCATATTAGATACCCA+GGG - chr1_3:62485308-62485327 Msa0131610:intron 40.0%
AAAATGTAGAAGAGAGGGAG+CGG - chr1_3:62485372-62485391 Msa0131610:CDS 40.0%
GTAGGAAGACTCAACGTATT+CGG - chr1_3:62485416-62485435 Msa0131610:intron 40.0%
TTGTGCCTTTCTAGATCGAT+TGG - chr1_3:62485559-62485578 Msa0131610:CDS 40.0%
TGTCACCAATCGATCTAGAA+AGG + chr1_3:62485567-62485586 None:intergenic 40.0%
AACGATATACTCTCCCACAT+CGG + chr1_3:62485590-62485609 None:intergenic 40.0%
ACCACATGTTAAGCAAGTGA+AGG - chr1_3:62485615-62485634 Msa0131610:CDS 40.0%
ACCTTCACTTGCTTAACATG+TGG + chr1_3:62485619-62485638 None:intergenic 40.0%
TTAAGCAAGTGAAGGTGAGT+TGG - chr1_3:62485623-62485642 Msa0131610:CDS 40.0%
! CTAGTGACCCTTTTATGTGA+AGG - chr1_3:62485667-62485686 Msa0131610:CDS 40.0%
! GTTTCCTAGCTTTCAAATGC+TGG - chr1_3:62485732-62485751 Msa0131610:intron 40.0%
TGTTTCTTTCTTGGAGTTGC+TGG + chr1_3:62486054-62486073 None:intergenic 40.0%
GTGCTTAGTTCACTTCTCTT+TGG - chr1_3:62486078-62486097 Msa0131610:intron 40.0%
TGTGGAGGAGTTATTTGAAG+AGG - chr1_3:62486158-62486177 Msa0131610:intron 40.0%
GTGGAGGAGTTATTTGAAGA+GGG - chr1_3:62486159-62486178 Msa0131610:intron 40.0%
TGCCATCATCAACAAGTGAT+TGG - chr1_3:62486272-62486291 Msa0131610:intron 40.0%
! GTTTAGGGTCTGTTTACTTC+GGG - chr1_3:62486304-62486323 Msa0131610:intron 40.0%
CTGTCATTGAGAGAACATGA+AGG + chr1_3:62486491-62486510 None:intergenic 40.0%
!!! TTACTTTTTACATCGGCAGC+TGG + chr1_3:62486542-62486561 None:intergenic 40.0%
CTTAAAATGCAGGCTAGGTA+AGG - chr1_3:62486647-62486666 Msa0131610:intron 40.0%
! CACCGATCATTTTATCACTC+CGG - chr1_3:62486755-62486774 Msa0131610:intron 40.0%
!! GCAATGCTTGGTTCTACATT+TGG - chr1_3:62486943-62486962 Msa0131610:intron 40.0%
AAGTGTGTGTGTGACAAATG+AGG - chr1_3:62486980-62486999 Msa0131610:intron 40.0%
GGTTGACAATTGAGTTAGCT+AGG - chr1_3:62487001-62487020 Msa0131610:intron 40.0%
! GCCTTTTGTGAAGTATGGAC+AGG - chr1_3:62484537-62484556 Msa0131610:intron 45.0%
GCCTGTCCATACTTCACAAA+AGG + chr1_3:62484541-62484560 None:intergenic 45.0%
TTTGTGAAGTATGGACAGGC+TGG - chr1_3:62484541-62484560 Msa0131610:intron 45.0%
!! CAGGCTGGTTTGGCAAAAAA+AGG - chr1_3:62484556-62484575 Msa0131610:intron 45.0%
ACAGATTGCCATAGAGTTCC+TGG - chr1_3:62484601-62484620 Msa0131610:intron 45.0%
AAGAATCGCCAGGAACTCTA+TGG + chr1_3:62484612-62484631 None:intergenic 45.0%
AATGACGTTGAAGAATCGCC+AGG + chr1_3:62484622-62484641 None:intergenic 45.0%
TTCTGCTTCGAGAGACCAAA+GGG - chr1_3:62484809-62484828 Msa0131610:CDS 45.0%
CTTGATTGGCAGATCCTTGA+CGG - chr1_3:62484964-62484983 Msa0131610:CDS 45.0%
TTGATTGGCAGATCCTTGAC+GGG - chr1_3:62484965-62484984 Msa0131610:CDS 45.0%
!!! ATCCGCTGCTATTTTTGCGA+AGG - chr1_3:62484999-62485018 Msa0131610:intron 45.0%
ACCGTTACAGTTGCATCTGT+TGG - chr1_3:62485278-62485297 Msa0131610:intron 45.0%
CCCAACAGATGCAACTGTAA+CGG + chr1_3:62485282-62485301 None:intergenic 45.0%
CCGTTACAGTTGCATCTGTT+GGG - chr1_3:62485279-62485298 Msa0131610:intron 45.0%
CGTTACAGTTGCATCTGTTG+GGG - chr1_3:62485280-62485299 Msa0131610:intron 45.0%
CCGATGCATATTAGATACCC+AGG - chr1_3:62485307-62485326 Msa0131610:intron 45.0%
TGCATATTAGATACCCAGGG+AGG - chr1_3:62485311-62485330 Msa0131610:intron 45.0%
CTTGACAGTTGATCACAGAC+TGG - chr1_3:62485346-62485365 Msa0131610:CDS 45.0%
AAATGTAGAAGAGAGGGAGC+GGG - chr1_3:62485373-62485392 Msa0131610:CDS 45.0%
! TCCTAGCTTTCAAATGCTGG+CGG - chr1_3:62485735-62485754 Msa0131610:intron 45.0%
AACCTGAACAGGAAGCTCTA+AGG - chr1_3:62485953-62485972 Msa0131610:CDS 45.0%
!! AGGAAGCTCTAAGGTCAAGA+GGG - chr1_3:62485962-62485981 Msa0131610:CDS 45.0%
TTTCTTGGAGTTGCTGGCAT+CGG + chr1_3:62486048-62486067 None:intergenic 45.0%
! GCTTTCTGCAGTTGGTATTG+TGG - chr1_3:62486140-62486159 Msa0131610:intron 45.0%
! TTCTGCAGTTGGTATTGTGG+AGG - chr1_3:62486143-62486162 Msa0131610:intron 45.0%
GTTCTGCAATGCTTACAGAG+AGG - chr1_3:62486181-62486200 Msa0131610:intron 45.0%
ACTGGTAAACCATCACCAGA+TGG + chr1_3:62486732-62486751 None:intergenic 45.0%
TGACAGTTTGTGCAGAGGAA+TGG + chr1_3:62486801-62486820 None:intergenic 45.0%
TCTTCTGACAGTTTGTGCAG+AGG + chr1_3:62486806-62486825 None:intergenic 45.0%
TCAGAAGAAGAAAGACGCCA+TGG - chr1_3:62486818-62486837 Msa0131610:intron 45.0%
AAGAAGAAAGACGCCATGGA+AGG - chr1_3:62486822-62486841 Msa0131610:intron 45.0%
AAGACGCCATGGAAGGAAAA+AGG - chr1_3:62486829-62486848 Msa0131610:intron 45.0%
! TCAGGACCTTTTTCCTTCCA+TGG + chr1_3:62486838-62486857 None:intergenic 45.0%
!! TGCTTGGTTCTACATTTGGG+TGG - chr1_3:62486947-62486966 Msa0131610:intron 45.0%
AGCTAGGTTAGGTCCATTGA+AGG - chr1_3:62487017-62487036 Msa0131610:five_prime_UTR 45.0%
TGTGCTCCCAACACCCATTA+GGG - chr1_3:62483871-62483890 Msa0131610:three_prime_UTR 50.0%
GAAGCAACCACTTGTTCCGT+TGG - chr1_3:62484471-62484490 Msa0131610:intron 50.0%
AAGGTTGCCAACGGAACAAG+TGG + chr1_3:62484481-62484500 None:intergenic 50.0%
GTTCCGTTGGCAACCTTGAT+CGG - chr1_3:62484484-62484503 Msa0131610:intron 50.0%
! GAAGTATGGACAGGCTGGTT+TGG - chr1_3:62484546-62484565 Msa0131610:intron 50.0%
GTTCTGCTTCGAGAGACCAA+AGG - chr1_3:62484808-62484827 Msa0131610:CDS 50.0%
! CAGATCCTTGACGGGCATAA+TGG - chr1_3:62484973-62484992 Msa0131610:CDS 50.0%
CATACCGCAAGCTGTAAGCA+GGG - chr1_3:62485050-62485069 Msa0131610:intron 50.0%
AAGCTGTAAGCAGGGAGGAA+TGG - chr1_3:62485058-62485077 Msa0131610:intron 50.0%
! AATGGCTTCAAGCACTGCCT+CGG - chr1_3:62485076-62485095 Msa0131610:intron 50.0%
GAGAGAAACAACACCTCCCT+GGG + chr1_3:62485327-62485346 None:intergenic 50.0%
AGAGAGAAACAACACCTCCC+TGG + chr1_3:62485328-62485347 None:intergenic 50.0%
TCTTCCTACTTCACCACCAC+TGG + chr1_3:62485405-62485424 None:intergenic 50.0%
GGAAGACTCAACGTATTCGG+AGG - chr1_3:62485419-62485438 Msa0131610:intron 50.0%
CAACGTATTCGGAGGCAATG+AGG - chr1_3:62485427-62485446 Msa0131610:intron 50.0%
! CTAGAAAGGCACAATCCACC+AGG + chr1_3:62485553-62485572 None:intergenic 50.0%
! ATCGATTGGTGACACCGATG+TGG - chr1_3:62485573-62485592 Msa0131610:CDS 50.0%
! TCGATTGGTGACACCGATGT+GGG - chr1_3:62485574-62485593 Msa0131610:CDS 50.0%
TCCGCCAGCATTTGAAAGCT+AGG + chr1_3:62485739-62485758 None:intergenic 50.0%
AGGTACACCACGACATGACT+TGG + chr1_3:62485817-62485836 None:intergenic 50.0%
CATCACCTTAACCACCAGCT+TGG + chr1_3:62485853-62485872 None:intergenic 50.0%
CAAGCTGGTGGTTAAGGTGA+TGG - chr1_3:62485851-62485870 Msa0131610:CDS 50.0%
GACCTTAGAGCTTCCTGTTC+AGG + chr1_3:62485958-62485977 None:intergenic 50.0%
!! CAGGAAGCTCTAAGGTCAAG+AGG - chr1_3:62485961-62485980 Msa0131610:CDS 50.0%
! TCTAAGGTCAAGAGGGCTGA+AGG - chr1_3:62485969-62485988 Msa0131610:intron 50.0%
GGCAACACAGGATAGTCTGA+AGG + chr1_3:62486027-62486046 None:intergenic 50.0%
TGCCTAGCAGATCAACCATC+TGG - chr1_3:62486714-62486733 Msa0131610:intron 50.0%
CACCAGATGGTTGATCTGCT+AGG + chr1_3:62486719-62486738 None:intergenic 50.0%
GCAGATCAACCATCTGGTGA+TGG - chr1_3:62486720-62486739 Msa0131610:intron 50.0%
TCACTCCGGTATCTAAGCCA+TGG - chr1_3:62486769-62486788 Msa0131610:intron 50.0%
CACTCCGGTATCTAAGCCAT+GGG - chr1_3:62486770-62486789 Msa0131610:intron 50.0%
CCTTCCCATGGCTTAGATAC+CGG + chr1_3:62486777-62486796 None:intergenic 50.0%
ACTGTTGTTGCTCGGACTTC+AGG + chr1_3:62486856-62486875 None:intergenic 50.0%
! AAGCATTGCTCACACTGCCA+AGG + chr1_3:62486932-62486951 None:intergenic 50.0%
TTGGCAGTGTGAGCAATGCT+TGG - chr1_3:62486931-62486950 Msa0131610:intron 50.0%
!! TTTGGCTTGTGCTCAGCTGT+TGG - chr1_3:62487056-62487075 Msa0131610:five_prime_UTR 50.0%
CTGTGCTCCCAACACCCATT+AGG - chr1_3:62483870-62483889 Msa0131610:three_prime_UTR 55.0%
ATCGGCCGTGAGCTACGAAA+TGG - chr1_3:62484502-62484521 Msa0131610:intron 55.0%
CCTGCTTACAGCTTGCGGTA+TGG + chr1_3:62485052-62485071 None:intergenic 55.0%
CCATACCGCAAGCTGTAAGC+AGG - chr1_3:62485049-62485068 Msa0131610:intron 55.0%
TCCTCCCTGCTTACAGCTTG+CGG + chr1_3:62485057-62485076 None:intergenic 55.0%
! ATGGCTTCAAGCACTGCCTC+GGG - chr1_3:62485077-62485096 Msa0131610:intron 55.0%
AAAGCGACAACTAGAGCCCG+AGG + chr1_3:62485096-62485115 None:intergenic 55.0%
TCACCTGCAGGGGAAACTTC+TGG - chr1_3:62485221-62485240 Msa0131610:intron 55.0%
GTTCCAGAAGTTTCCCCTGC+AGG + chr1_3:62485227-62485246 None:intergenic 55.0%
! ACAGCCAGTGGTGGTGAAGT+AGG - chr1_3:62485398-62485417 Msa0131610:CDS 55.0%
AGCTTGGCAGCAAGTTCTGC+AGG + chr1_3:62485837-62485856 None:intergenic 55.0%
!! TGCAGAACTTGCTGCCAAGC+TGG - chr1_3:62485836-62485855 Msa0131610:CDS 55.0%
!! AGAACTTGCTGCCAAGCTGG+TGG - chr1_3:62485839-62485858 Msa0131610:CDS 55.0%
TGCTGCCAAGCTGGTGGTTA+AGG - chr1_3:62485845-62485864 Msa0131610:CDS 55.0%
CTGCTAGGCAAACTGCACAG+CGG + chr1_3:62486704-62486723 None:intergenic 55.0%
CCGGTATCTAAGCCATGGGA+AGG - chr1_3:62486774-62486793 Msa0131610:intron 55.0%
CAGAGGAATGGACCTTCCCA+TGG + chr1_3:62486789-62486808 None:intergenic 55.0%
CGGCCGATCAAGGTTGCCAA+CGG + chr1_3:62484490-62484509 None:intergenic 60.0%
TCGTAGCTCACGGCCGATCA+AGG + chr1_3:62484500-62484519 None:intergenic 60.0%
ACCGCAAGCTGTAAGCAGGG+AGG - chr1_3:62485053-62485072 Msa0131610:intron 60.0%
AGGTAGGGCCTCTTCGCTGT+TGG - chr1_3:62485527-62485546 Msa0131610:CDS 60.0%
ACGGCTGCCAAGTCATGTCG+TGG - chr1_3:62485807-62485826 Msa0131610:intron 60.0%
!! GTTGCTGGCATCGGCAACAC+AGG + chr1_3:62486039-62486058 None:intergenic 60.0%
!! AGGGAGCGGGTTACAGCCAG+TGG - chr1_3:62485386-62485405 Msa0131610:CDS 65.0%
!! GAGCGGGTTACAGCCAGTGG+TGG - chr1_3:62485389-62485408 Msa0131610:CDS 65.0%
CCACCAGGCCAACAGCGAAG+AGG + chr1_3:62485538-62485557 None:intergenic 65.0%
! CCTCTTCGCTGTTGGCCTGG+TGG - chr1_3:62485535-62485554 Msa0131610:CDS 65.0%
GGGCCTCTTCGCTGTTGGCC+TGG - chr1_3:62485532-62485551 Msa0131610:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 62483742 62487218 62483742 ID=Msa0131610;Name=Msa0131610
chr1_3 mRNA 62483742 62487218 62483742 ID=Msa0131610-mRNA-1;Parent=Msa0131610;Name=Msa0131610-mRNA-1;_AED=0.07;_eAED=0.07;_QI=248|1|1|1|0.85|0.75|8|361|423
chr1_3 exon 62483742 62484301 62483742 ID=Msa0131610-mRNA-1:exon:20865;Parent=Msa0131610-mRNA-1
chr1_3 exon 62484758 62484996 62484758 ID=Msa0131610-mRNA-1:exon:20864;Parent=Msa0131610-mRNA-1
chr1_3 exon 62485094 62485219 62485094 ID=Msa0131610-mRNA-1:exon:20863;Parent=Msa0131610-mRNA-1
chr1_3 exon 62485324 62485431 62485324 ID=Msa0131610-mRNA-1:exon:20862;Parent=Msa0131610-mRNA-1
chr1_3 exon 62485512 62485729 62485512 ID=Msa0131610-mRNA-1:exon:20861;Parent=Msa0131610-mRNA-1
chr1_3 exon 62485813 62485984 62485813 ID=Msa0131610-mRNA-1:exon:20860;Parent=Msa0131610-mRNA-1
chr1_3 exon 62486306 62486548 62486306 ID=Msa0131610-mRNA-1:exon:20859;Parent=Msa0131610-mRNA-1
chr1_3 exon 62487004 62487218 62487004 ID=Msa0131610-mRNA-1:exon:20858;Parent=Msa0131610-mRNA-1
chr1_3 five_prime_UTR 62487004 62487218 62487004 ID=Msa0131610-mRNA-1:five_prime_utr;Parent=Msa0131610-mRNA-1
chr1_3 five_prime_UTR 62486516 62486548 62486516 ID=Msa0131610-mRNA-1:five_prime_utr;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62486306 62486515 62486306 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62485813 62485984 62485813 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62485512 62485729 62485512 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62485324 62485431 62485324 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62485094 62485219 62485094 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62484758 62484996 62484758 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 CDS 62484103 62484301 62484103 ID=Msa0131610-mRNA-1:cds;Parent=Msa0131610-mRNA-1
chr1_3 three_prime_UTR 62483742 62484102 62483742 ID=Msa0131610-mRNA-1:three_prime_utr;Parent=Msa0131610-mRNA-1
Gene Sequence

>Msa0131610

ATGAGTAAGACTGAATTATCAAGGATGAAGCAACCACTTGTTCCGTTGGCAACCTTGATCGGCCGTGAGCTACGAAATGGAAAAACGGAGAAGCCTTTTGTGAAGTATGGACAGGCTGGTTTGGCAAAAAAAGGAGAAGATTACTTTCTAATCAAGACAGATTGCCATAGAGTTCCTGGCGATTCTTCAACGTCATTTTCTGTTTTCGCGATCCTTGACGGGCATAATGGTATATCCGCTGCTATTTTTGCGAAGGAAAACATAATAAACAATGTCATGAGTGCCATACCGCAAGCTGTAAGCAGGGAGGAATGGCTTCAAGCACTGCCTCGGGCTCTAGTTGTCGCTTTTGTGAAAACTGACATGGAATTTCAGAAAAAAGGGGAAACTTCTGGAACGACAGCTACGTTTGTGATTATTGATGGATGGACCGTTACAGTTGCATCTGTTGGGGATTCCCGATGCATATTAGATACCCAGGGAGGTGTTGTTTCTCTCTTGACAGTTGATCACAGACTGGAAGAAAATGTAGAAGAGAGGGAGCGGGTTACAGCCAGTGGTGGTGAAGTAGGAAGACTCAACGTATTCGGAGGCAATGAGGTAGGGCCTCTTCGCTGTTGGCCTGGTGGATTGTGCCTTTCTAGATCGATTGGTGACACCGATGTGGGAGAGTATATCGTTCCAATACCACATGTTAAGCAAGTGAAGCTTTCAAATGCTGGCGGAAGACTAATTATAGCTTCTGATGGAATTTGGGATACTTTGTCTTCTGATACGGCTGCCAAGTCATGTCGTGGTGTACCTGCAGAACTTGCTGCCAAGCTGGTGGTTAAGGAAGCTCTAAGGTCAAGAGGGCTGAAGGATGATACAACCTGCCTTGTTGTAGATATTATTCCTTCAGACTATCCTGTGTTGCCGATGCCAGCAACTCCAAGAAAGAAACATAATGTGCTTAGTTCACTTCTCTTTGGAAAGAAATCTCAAAACTCGACAAACAAAGGAACTAATAAGCTTTCTGCAGTTGGTATTGTGGAGGAGTTATTTGAAGAGGGTTCTGCAATGCTTACAGAGAGGCTAGGTAAGGATGTTCCTTCCAATACAAATTCTGGTATTCACCGCTGTGCAGTTTGCCTAGCAGATCAACCATCTGGTGATGGTTTACCAGTGAACACCGATCATTTTATCACTCCGGTATCTAAGCCATGGGAAGGTCCATTCCTCTGCACAAACTGTCAGAAGAAGAAAGACGCCATGGAAGGAAAAAGGTCCTGA
Protein sequence

>Msa0131610

MSKTELSRMKQPLVPLATLIGRELRNGKTEKPFVKYGQAGLAKKGEDYFLIKTDCHRVPGDSSTSFSVFAILDGHNGISAAIFAKENIINNVMSAIPQAVSREEWLQALPRALVVAFVKTDMEFQKKGETSGTTATFVIIDGWTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENVEERERVTASGGEVGRLNVFGGNEVGPLRCWPGGLCLSRSIGDTDVGEYIVPIPHVKQVKLSNAGGRLIIASDGIWDTLSSDTAAKSCRGVPAELAAKLVVKEALRSRGLKDDTTCLVVDIIPSDYPVLPMPATPRKKHNVLSSLLFGKKSQNSTNKGTNKLSAVGIVEELFEEGSAMLTERLGKDVPSNTNSGIHRCAVCLADQPSGDGLPVNTDHFITPVSKPWEGPFLCTNCQKKKDAMEGKRS*