AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0173030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0173030 MtrunA17_Chr1g0192471 97.400 423 11 0 1 423 1 423 0.0 848
Msa0173030 MtrunA17_Chr7g0271971 85.308 422 60 2 1 422 1 420 0.0 716
Msa0173030 MtrunA17_Chr1g0209451 68.632 424 129 3 1 422 1 422 0.0 592
Msa0173030 MtrunA17_Chr1g0153671 56.341 410 177 2 14 423 11 418 1.61e-168 479
Msa0173030 MtrunA17_Chr7g0225501 62.673 434 137 13 1 422 19 439 8.25e-167 475
Msa0173030 MtrunA17_Chr3g0133801 55.556 414 175 3 14 422 10 419 3.67e-163 466
Msa0173030 MtrunA17_Chr2g0326251 64.486 214 61 8 109 317 7 210 7.66e-74 230
Msa0173030 MtrunA17_Chr1g0150451 31.802 283 154 12 25 298 72 324 9.91e-29 115
Msa0173030 MtrunA17_Chr3g0136711 31.325 249 143 9 57 298 54 281 1.79e-27 110
Msa0173030 MtrunA17_Chr1g0212961 31.673 281 155 14 25 299 90 339 1.50e-25 106
Msa0173030 MtrunA17_Chr3g0105351 30.147 272 162 11 33 297 64 314 2.29e-25 105
Msa0173030 MtrunA17_Chr3g0105371 30.403 273 160 10 33 297 88 338 3.57e-25 105
Msa0173030 MtrunA17_Chr5g0430481 28.625 269 164 11 36 297 127 374 1.73e-22 98.2
Msa0173030 MtrunA17_Chr8g0360351 30.043 233 139 8 68 295 66 279 3.54e-21 92.8
Msa0173030 MtrunA17_Chr2g0317021 29.572 257 152 11 46 297 100 332 3.17e-20 91.3
Msa0173030 MtrunA17_Chr4g0070871 27.365 296 159 10 46 297 220 503 6.98e-20 91.7
Msa0173030 MtrunA17_Chr3g0126511 29.020 255 152 9 46 295 108 338 2.47e-19 89.0
Msa0173030 MtrunA17_Chr4g0050381 27.698 278 168 10 27 295 21 274 4.46e-19 87.0
Msa0173030 MtrunA17_Chr1g0171571 31.301 246 136 7 69 299 419 646 4.51e-19 89.7
Msa0173030 MtrunA17_Chr3g0115851 29.032 279 163 11 27 295 26 279 2.94e-18 84.7
Msa0173030 MtrunA17_Chr2g0279151 25.818 275 177 9 27 295 25 278 3.97e-18 84.3
Msa0173030 MtrunA17_Chr5g0407161 26.894 264 153 9 67 314 123 362 4.87e-18 85.5
Msa0173030 MtrunA17_Chr5g0426141 29.749 279 161 12 27 295 26 279 1.03e-17 82.8
Msa0173030 MtrunA17_Chr5g0396731 29.524 210 123 7 66 265 105 299 2.25e-17 83.2
Msa0173030 MtrunA17_Chr1g0150781 30.712 267 137 10 69 299 291 545 1.11e-16 82.0
Msa0173030 MtrunA17_Chr4g0051661 29.921 254 150 10 46 295 166 395 1.16e-16 81.3
Msa0173030 MtrunA17_Chr8g0389921 29.949 197 117 6 69 258 199 381 1.82e-16 81.3
Msa0173030 MtrunA17_Chr3g0112291 29.032 217 122 6 62 261 117 318 1.97e-16 80.5
Msa0173030 MtrunA17_Chr6g0466641 87.805 41 5 0 1 41 1 41 2.72e-16 73.9
Msa0173030 MtrunA17_Chr2g0299981 25.857 321 162 10 40 297 228 535 3.16e-16 80.9
Msa0173030 MtrunA17_Chr4g0065141 30.040 253 130 11 65 295 121 348 5.19e-16 79.3
Msa0173030 MtrunA17_Chr6g0483411 28.053 303 165 13 26 295 34 316 5.27e-16 78.6
Msa0173030 MtrunA17_Chr2g0293811 31.373 255 131 11 64 295 96 329 6.97e-16 78.6
Msa0173030 MtrunA17_Chr3g0135961 27.574 272 149 8 62 297 280 539 1.22e-15 79.0
Msa0173030 MtrunA17_Chr1g0158781 31.048 248 138 9 69 297 146 379 9.63e-15 75.5
Msa0173030 MtrunA17_Chr7g0272061 33.898 177 95 9 116 276 165 335 1.04e-14 75.1
Msa0173030 MtrunA17_Chr5g0435721 28.019 207 123 7 69 262 224 417 4.78e-14 73.9
Msa0173030 MtrunA17_Chr7g0221691 30.403 273 142 14 36 283 139 388 9.96e-14 72.8
Msa0173030 MtrunA17_Chr3g0138281 27.969 261 145 9 67 308 120 356 1.94e-13 71.6
Msa0173030 MtrunA17_Chr6g0486661 27.181 298 168 14 36 297 97 381 2.78e-13 72.0
Msa0173030 MtrunA17_Chr4g0066371 31.126 302 148 19 36 297 59 340 4.27e-13 70.5
Msa0173030 MtrunA17_Chr5g0394181 27.907 258 140 11 66 297 360 597 1.19e-12 69.7
Msa0173030 MtrunA17_Chr2g0286951 27.431 288 163 12 43 297 67 341 2.79e-12 68.2
Msa0173030 MtrunA17_Chr4g0001761 34.043 141 66 5 128 257 156 280 3.62e-12 67.4
Msa0173030 MtrunA17_Chr8g0342431 30.189 159 94 7 136 283 201 353 3.04e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0173030 AT1G09160.2 75.355 422 102 1 1 422 1 420 0.0 646
Msa0173030 AT1G09160.1 75.355 422 102 1 1 422 1 420 0.0 646
Msa0173030 AT1G68410.4 68.675 415 126 2 11 422 16 429 0.0 580
Msa0173030 AT1G68410.3 68.675 415 126 2 11 422 16 429 0.0 580
Msa0173030 AT1G68410.1 68.675 415 126 2 11 422 16 429 0.0 580
Msa0173030 AT1G68410.2 68.675 415 126 2 11 422 16 429 0.0 580
Msa0173030 AT1G47380.1 56.341 410 178 1 14 423 10 418 8.58e-170 483
Msa0173030 AT5G10740.1 31.250 304 167 13 6 298 8 280 2.53e-27 112
Msa0173030 AT5G10740.2 31.250 304 167 13 6 298 8 280 2.53e-27 112
Msa0173030 AT2G34740.1 33.054 239 136 8 65 298 116 335 8.02e-27 110
Msa0173030 AT5G24940.1 31.228 285 153 13 25 298 28 280 1.05e-25 108
Msa0173030 AT1G07160.1 30.686 277 157 11 33 297 123 376 1.28e-25 107
Msa0173030 AT1G67820.2 32.787 244 139 10 34 270 78 303 1.30e-25 108
Msa0173030 AT4G31750.1 29.508 305 162 13 25 306 28 302 1.58e-25 106
Msa0173030 AT4G31750.3 29.508 305 162 13 25 306 28 302 1.58e-25 106
Msa0173030 AT1G67820.1 32.787 244 139 10 34 270 114 339 1.66e-25 108
Msa0173030 AT4G31750.2 30.466 279 157 11 25 295 28 277 1.97e-25 105
Msa0173030 AT1G22280.3 30.901 233 143 7 68 295 65 284 5.56e-24 101
Msa0173030 AT1G43900.1 31.599 269 149 14 36 295 124 366 5.94e-24 102
Msa0173030 AT2G40180.1 28.269 283 183 8 21 297 117 385 8.87e-24 102
Msa0173030 AT1G34750.2 29.749 279 161 11 27 295 26 279 1.69e-23 99.8
Msa0173030 AT1G34750.1 29.749 279 161 11 27 295 26 279 1.69e-23 99.8
Msa0173030 AT1G34750.3 29.749 279 161 11 27 295 26 279 1.69e-23 99.8
Msa0173030 AT1G78200.1 30.357 280 161 11 27 295 24 280 1.92e-23 99.8
Msa0173030 AT1G78200.3 30.357 280 161 11 27 295 24 280 1.92e-23 99.8
Msa0173030 AT1G78200.2 30.357 280 161 11 27 295 24 280 1.92e-23 99.8
Msa0173030 AT1G78200.5 31.128 257 147 10 46 295 2 235 2.09e-23 98.6
Msa0173030 AT1G78200.4 31.128 257 147 10 46 295 2 235 2.09e-23 98.6
Msa0173030 AT2G30020.1 28.782 271 163 11 36 297 143 392 9.92e-23 99.8
Msa0173030 AT4G28400.3 29.258 229 146 6 68 295 45 258 1.01e-22 97.1
Msa0173030 AT5G53140.1 29.412 255 151 10 46 295 115 345 1.35e-22 99.8
Msa0173030 AT5G53140.2 29.412 255 151 10 46 295 115 345 1.35e-22 99.8
Msa0173030 AT4G28400.1 29.258 229 146 6 68 295 67 280 1.97e-22 97.1
Msa0173030 AT1G22280.1 30.901 233 137 8 68 295 65 278 3.47e-22 96.3
Msa0173030 AT2G20630.1 29.104 268 158 12 33 295 36 276 5.00e-22 95.5
Msa0173030 AT4G26080.1 27.737 274 149 7 61 299 165 424 1.72e-21 96.7
Msa0173030 AT2G20630.2 28.839 267 158 12 33 294 36 275 2.25e-21 94.0
Msa0173030 AT3G51470.2 28.475 295 169 11 36 314 32 300 5.75e-21 93.6
Msa0173030 AT3G51470.1 28.475 295 169 11 36 314 76 344 6.72e-21 94.0
Msa0173030 AT5G57050.2 28.464 267 145 9 65 297 117 371 4.68e-20 91.7
Msa0173030 AT5G57050.1 28.464 267 145 9 65 297 157 411 6.00e-20 91.7
Msa0173030 AT2G40860.1 29.286 280 157 9 36 299 396 650 1.20e-19 92.0
Msa0173030 AT2G40860.4 29.286 280 157 9 36 299 298 552 1.98e-19 90.9
Msa0173030 AT2G25620.1 30.075 266 137 10 67 308 127 367 2.41e-19 89.7
Msa0173030 AT2G25620.2 30.075 266 137 10 67 308 127 367 2.41e-19 89.7
Msa0173030 AT2G34740.3 29.665 209 127 5 65 270 112 303 3.21e-19 88.2
Msa0173030 AT2G34740.2 29.665 209 127 5 65 270 130 321 3.23e-19 88.6
Msa0173030 AT3G62260.3 29.771 262 148 12 67 307 26 272 6.23e-18 84.3
Msa0173030 AT2G40860.2 28.409 264 148 8 36 282 396 635 9.36e-18 85.9
Msa0173030 AT3G62260.1 29.771 262 148 12 67 307 115 361 1.82e-17 84.0
Msa0173030 AT1G48040.1 32.692 260 129 12 63 295 107 347 1.84e-17 84.0
Msa0173030 AT3G62260.2 29.771 262 148 12 67 307 116 362 1.87e-17 84.0
Msa0173030 AT3G15260.2 30.342 234 137 9 68 295 73 286 3.58e-17 82.0
Msa0173030 AT3G15260.1 30.342 234 137 9 68 295 73 286 3.58e-17 82.0
Msa0173030 AT1G34750.4 28.085 235 136 9 27 252 26 236 1.26e-16 79.3
Msa0173030 AT5G59220.1 28.287 251 150 10 31 265 107 343 3.60e-15 77.4
Msa0173030 AT1G18030.1 29.020 255 135 10 69 295 110 346 3.91e-15 76.6
Msa0173030 AT4G28400.2 27.568 185 120 4 68 252 67 237 5.95e-15 74.7
Msa0173030 AT1G07430.1 28.054 221 126 7 62 265 151 355 4.65e-14 73.9
Msa0173030 AT4G27800.1 24.848 330 173 12 40 322 67 368 7.92e-14 73.2
Msa0173030 AT3G11410.1 25.446 224 126 7 62 260 131 338 1.90e-12 68.9
Msa0173030 AT2G40860.3 29.114 237 127 8 36 255 396 608 2.53e-12 69.3
Msa0173030 AT4G27800.2 26.119 268 134 9 40 265 67 312 3.20e-12 67.8
Msa0173030 AT4G27800.3 26.119 268 134 9 40 265 67 312 3.20e-12 67.8
Msa0173030 AT1G16220.1 23.580 352 196 13 5 301 45 378 5.58e-12 67.8
Msa0173030 AT3G17250.1 27.668 253 141 13 67 295 157 391 5.80e-12 67.4
Msa0173030 AT5G27930.3 34.146 164 90 8 100 251 144 301 7.07e-12 66.6
Msa0173030 AT1G17550.1 27.876 226 119 8 69 265 238 448 7.11e-12 67.4
Msa0173030 AT1G17550.2 27.876 226 119 8 69 265 238 448 7.11e-12 67.4
Msa0173030 AT1G79630.2 29.798 198 121 6 131 314 151 344 1.41e-11 66.2
Msa0173030 AT1G79630.7 29.798 198 121 6 131 314 151 344 1.41e-11 66.2
Msa0173030 AT1G79630.6 29.500 200 123 6 131 316 111 306 1.66e-11 65.9
Msa0173030 AT1G79630.3 29.500 200 123 6 131 316 111 306 1.66e-11 65.9
Msa0173030 AT1G79630.4 29.500 200 123 6 131 316 111 306 1.66e-11 65.9
Msa0173030 AT1G79630.5 29.500 200 123 6 131 316 111 306 1.66e-11 65.9
Msa0173030 AT1G79630.8 29.500 200 123 6 131 316 217 412 1.75e-11 66.2
Msa0173030 AT1G79630.1 29.500 200 123 6 131 316 221 416 2.54e-11 65.9
Msa0173030 AT1G18030.2 30.288 208 104 9 69 253 110 299 3.15e-11 64.3
Msa0173030 AT2G29380.1 27.928 222 132 8 59 265 103 311 3.85e-11 64.7
Msa0173030 AT4G32950.1 30.114 176 110 5 131 297 138 309 4.08e-11 64.3
Msa0173030 AT5G51760.2 24.900 249 153 6 40 265 86 323 6.28e-11 64.3
Msa0173030 AT5G51760.1 24.900 249 153 6 40 265 116 353 6.42e-11 64.3
Msa0173030 AT5G36250.1 25.778 225 137 9 100 300 171 389 6.76e-11 64.3
Msa0173030 AT1G72770.4 25.090 279 143 11 69 297 239 501 7.32e-11 64.3
Msa0173030 AT1G72770.1 25.090 279 143 11 69 297 239 501 7.32e-11 64.3
Msa0173030 AT1G72770.3 25.090 279 143 11 69 297 239 501 7.32e-11 64.3

Find 138 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGCTTCTGATGGAATT+TGG 0.123861 1_4:-58218409 Msa0173030:CDS
AGCACATTATGTTTCTTTCT+TGG 0.197447 1_4:+58218109 None:intergenic
GTTCCTTCCAATACAAATTC+TGG 0.255485 1_4:-58217474 Msa0173030:CDS
TGTTTCTTTCTTGGAGTTGC+TGG 0.268275 1_4:+58218118 None:intergenic
CTGTGCTCTCAACACCCATT+AGG 0.273813 1_4:-58220307 Msa0173030:five_prime_UTR
GTGCTTAGTTCACTTCTCTT+TGG 0.288508 1_4:-58218091 Msa0173030:CDS
GCAATGCTTGGTTCTACATT+TGG 0.316695 1_4:-58217203 Msa0173030:three_prime_UTR
TCACCTGCAGGGGAAACTTC+TGG 0.319705 1_4:-58218955 Msa0173030:intron
TATAGCTTCTGATGGAATTT+GGG 0.324404 1_4:-58218408 Msa0173030:CDS
ACTGTTGTTGCTCGGACTTC+AGG 0.329448 1_4:+58217293 None:intergenic
GCTACGTTTGTGATTATTGA+TGG 0.331297 1_4:-58218925 Msa0173030:CDS
TGTGCTCTCAACACCCATTA+GGG 0.338960 1_4:-58220306 Msa0173030:five_prime_UTR
CCGTTACAGTTGCATCTGTT+GGG 0.351128 1_4:-58218897 Msa0173030:CDS
CAATGCTTGGTTCTACATTT+GGG 0.352020 1_4:-58217202 Msa0173030:three_prime_UTR
ACAGATTGCCATAGAGTTCC+TGG 0.368715 1_4:-58219575 Msa0173030:CDS
ACTTGCTTAACATGTGGTAT+TGG 0.372029 1_4:+58218565 None:intergenic
TGCTGCCAAGCTGGTGGTTA+AGG 0.391611 1_4:-58218330 Msa0173030:intron
TAATTTGCAACAACCTTCAA+TGG 0.406917 1_4:+58217116 None:intergenic
AAGACGCCATGGAAGGAAAA+AGG 0.416517 1_4:-58217317 Msa0173030:CDS
CAGATCCTTGACGGGCATAA+TGG 0.417208 1_4:-58219203 Msa0173030:CDS
GGTTGAGAATTGAGTTAGCT+AGG 0.417286 1_4:-58217145 Msa0173030:three_prime_UTR
GGGCCTCTTCGCTGTTGGCC+TGG 0.420476 1_4:-58218643 Msa0173030:CDS
AACCTGAACAGGAAGCTCTA+AGG 0.420809 1_4:-58218216 Msa0173030:intron
TTTGGCTTGTGCTCAGCTGT+TGG 0.425707 1_4:-58217090 Msa0173030:three_prime_UTR
GTAGGAAGACTCAACGTATT+CGG 0.426631 1_4:-58218760 Msa0173030:CDS
GTTTCCTAGCTTTCAAATGC+TGG 0.427993 1_4:-58218443 Msa0173030:intron
CTTGATTGGCAGATCCTTGA+CGG 0.435403 1_4:-58219212 Msa0173030:intron
TGGAAGAAAATGTAGAAGAG+AGG 0.437691 1_4:-58218810 Msa0173030:CDS
GTGAATACCAGAATTTGTAT+TGG 0.439513 1_4:+58217467 None:intergenic
CTTAAAATGCAGGCTAGGTA+AGG 0.443855 1_4:-58217499 Msa0173030:intron
ATGGCTTCAAGCACTGCCTC+GGG 0.448779 1_4:-58219099 Msa0173030:CDS
ACTAATAAGCTTTCTGCAGT+TGG 0.455037 1_4:-58218037 Msa0173030:CDS
TCGTAGCTCACGGCCGATCA+AGG 0.456909 1_4:+58219679 None:intergenic
GGATACTTTGTCTTCTGATA+CGG 0.461894 1_4:-58218387 Msa0173030:CDS
AATGGCTTCAAGCACTGCCT+CGG 0.462446 1_4:-58219100 Msa0173030:CDS
ATCGGCCGTGAGCTACGAAA+TGG 0.463670 1_4:-58219674 Msa0173030:CDS
GTTCCGTTGGCAACCTTGAT+CGG 0.465033 1_4:-58219692 Msa0173030:CDS
AGAATTGAGTTAGCTAGGTT+AGG 0.469351 1_4:-58217140 Msa0173030:three_prime_UTR
AAGAATCGCCAGGAACTCTA+TGG 0.471757 1_4:+58219567 None:intergenic
GCCTGTCCATACTTCACAAA+AGG 0.476504 1_4:+58219638 None:intergenic
TTTCTTGGAGTTGCTGGCAT+CGG 0.478380 1_4:+58218124 None:intergenic
GAAGTATGGACAGGCTGGTT+TGG 0.479502 1_4:-58219630 Msa0173030:CDS
GTGGAGGAGTTATTTGAAGA+GGG 0.481037 1_4:-58218010 Msa0173030:CDS
CTGGGTATCTAATATGCATC+GGG 0.481436 1_4:+58218870 None:intergenic
ACCGTTACAGTTGCATCTGT+TGG 0.483048 1_4:-58218898 Msa0173030:CDS
TGCAGAACTTGCTGCCAAGC+TGG 0.486136 1_4:-58218339 Msa0173030:CDS
TCTTCCTACTTCACCACCAC+TGG 0.489968 1_4:+58218774 None:intergenic
AATAATATCTACAACAAGGC+AGG 0.494608 1_4:+58218168 None:intergenic
GCTTTCTGCAGTTGGTATTG+TGG 0.496447 1_4:-58218029 Msa0173030:CDS
CCATACCGCAAGGTATAAGC+AGG 0.497999 1_4:-58219127 Msa0173030:CDS
AGCTAGGTTAGGTCCATTGA+AGG 0.499451 1_4:-58217129 Msa0173030:three_prime_UTR
TGACAGTTTGTGCAGAGGAA+TGG 0.499633 1_4:+58217348 None:intergenic
CCTTCCCATGGCTTAGATAC+CGG 0.499863 1_4:+58217372 None:intergenic
TGCCTAGCAGATCAACCATC+TGG 0.501426 1_4:-58217432 Msa0173030:CDS
TTTGTGAAGTATGGACAGGC+TGG 0.503204 1_4:-58219635 Msa0173030:CDS
AAAATGTAGAAGAGAGGGAG+CGG 0.505425 1_4:-58218804 Msa0173030:CDS
TATATTGATTTGATTGCTAA+CGG 0.510016 1_4:-58216951 Msa0173030:three_prime_UTR
CGTTTGTGATTATTGATGGA+TGG 0.510062 1_4:-58218921 Msa0173030:CDS
AGAGAGAAACAACACCTCCC+TGG 0.510635 1_4:+58218851 None:intergenic
TTGGCAGTGTGAGCAATGCT+TGG 0.513571 1_4:-58217215 Msa0173030:three_prime_UTR
TTCTGCAGTTGGTATTGTGG+AGG 0.515246 1_4:-58218026 Msa0173030:CDS
AGCTTGGCAGCAAGTTCTGC+AGG 0.516289 1_4:+58218341 None:intergenic
AGGTAGGGCCTCTTCGCTGT+TGG 0.519693 1_4:-58218648 Msa0173030:CDS
ACAGCCAGTGGTGGTGAAGT+AGG 0.520024 1_4:-58218778 Msa0173030:CDS
CGTTACAGTTGCATCTGTTG+GGG 0.531574 1_4:-58218896 Msa0173030:CDS
TTGATTGGCAGATCCTTGAC+GGG 0.533037 1_4:-58219211 Msa0173030:intron
GAAGCAACCACTTGTTCCGT+TGG 0.533409 1_4:-58219705 Msa0173030:CDS
TGAGTAAGACTGAATTATCA+AGG 0.534598 1_4:-58219730 Msa0173030:CDS
ATAAAATGATCGGTGTTCAC+TGG 0.535304 1_4:+58217399 None:intergenic
CCTCTTCGCTGTTGGCCTGG+TGG 0.536837 1_4:-58218640 Msa0173030:CDS
AAATGTAGAAGAGAGGGAGC+GGG 0.539423 1_4:-58218803 Msa0173030:CDS
TGTGGAGGAGTTATTTGAAG+AGG 0.540145 1_4:-58218011 Msa0173030:CDS
AATGACGTTGAAGAATCGCC+AGG 0.544088 1_4:+58219557 None:intergenic
CCTGGGTATCTAATATGCAT+CGG 0.544206 1_4:+58218869 None:intergenic
ATACCAGAATTTGTATTGGA+AGG 0.544653 1_4:+58217471 None:intergenic
CCTGCTTATACCTTGCGGTA+TGG 0.547950 1_4:+58219127 None:intergenic
TCACTCCGGTATCTAAGCCA+TGG 0.550001 1_4:-58217377 Msa0173030:CDS
AGACTAATTATAGCTTCTGA+TGG 0.551958 1_4:-58218416 Msa0173030:CDS
TCTAAGGTCAAGAGGGCTGA+AGG 0.552829 1_4:-58218200 Msa0173030:CDS
TGTCACCAATCGATCTAGAA+AGG 0.554127 1_4:+58218611 None:intergenic
GAGAGAAACAACACCTCCCT+GGG 0.554466 1_4:+58218852 None:intergenic
AGGGAGCGGGTTACAGCCAG+TGG 0.559334 1_4:-58218790 Msa0173030:CDS
AAGGTATAAGCAGGGAGGAA+TGG 0.559959 1_4:-58219118 Msa0173030:CDS
GGAAGAAAATGTAGAAGAGA+GGG 0.560448 1_4:-58218809 Msa0173030:CDS
CACTCCGGTATCTAAGCCAT+GGG 0.564768 1_4:-58217376 Msa0173030:CDS
CTAGAAAGGCACAATCCACC+AGG 0.566834 1_4:+58218625 None:intergenic
CCGATGCATATTAGATACCC+AGG 0.567386 1_4:-58218869 Msa0173030:CDS
TCTTCTGACAGTTTGTGCAG+AGG 0.570739 1_4:+58217343 None:intergenic
TTGTGCCTTTCTAGATCGAT+TGG 0.574242 1_4:-58218616 Msa0173030:CDS
TCCTCCCTGCTTATACCTTG+CGG 0.574320 1_4:+58219122 None:intergenic
CACCAGATGGTTGATCTGCT+AGG 0.577655 1_4:+58217430 None:intergenic
AGGTACACCACGACATGACT+TGG 0.578313 1_4:+58218361 None:intergenic
CAGGAAGCTCTAAGGTCAAG+AGG 0.580501 1_4:-58218208 Msa0173030:CDS
GTTGCTGGCATCGGCAACAC+AGG 0.580752 1_4:+58218133 None:intergenic
ACCTTCACTTGCTTAACATG+TGG 0.582513 1_4:+58218559 None:intergenic
CAGAGGAATGGACCTTCCCA+TGG 0.585428 1_4:+58217360 None:intergenic
TCTCAAAACTCGACAAACAA+AGG 0.587947 1_4:-58218061 Msa0173030:CDS
TGCTTGGTTCTACATTTGGG+TGG 0.590730 1_4:-58217199 Msa0173030:three_prime_UTR
ATAACTATACTGTTGTTGCT+CGG 0.593054 1_4:+58217285 None:intergenic
CTTGACAGTTGATCACAGAC+TGG 0.593422 1_4:-58218830 Msa0173030:CDS
AAGAAGAAAGACGCCATGGA+AGG 0.594109 1_4:-58217324 Msa0173030:CDS
ATCGATTGGTGACACCGATG+TGG 0.601496 1_4:-58218602 Msa0173030:CDS
GCAGATCAACCATCTGGTGA+TGG 0.602774 1_4:-58217426 Msa0173030:CDS
AAGCATTGCTCACACTGCCA+AGG 0.610583 1_4:+58217217 None:intergenic
TACCGGAGTGATAAAATGAT+CGG 0.613081 1_4:+58217389 None:intergenic
CATCACCTTAACCACCAGCT+TGG 0.613564 1_4:+58218325 None:intergenic
GAAATGTTGACTTATAAACT+AGG 0.614349 1_4:-58219754 Msa0173030:five_prime_UTR
CCACCAGGCCAACAGCGAAG+AGG 0.616089 1_4:+58218640 None:intergenic
TCGATTGGTGACACCGATGT+GGG 0.617709 1_4:-58218601 Msa0173030:CDS
AAGGTTGCCAACGGAACAAG+TGG 0.618923 1_4:+58219698 None:intergenic
ATATACCATTATGCCCGTCA+AGG 0.625718 1_4:+58219198 None:intergenic
GAGCGGGTTACAGCCAGTGG+TGG 0.626341 1_4:-58218787 Msa0173030:CDS
AGGAAGCTCTAAGGTCAAGA+GGG 0.627250 1_4:-58218207 Msa0173030:CDS
ACCACATGTTAAGCAAGTGA+AGG 0.627901 1_4:-58218560 Msa0173030:intron
GTCATGAGTGCCATACCGCA+AGG 0.630194 1_4:-58219137 Msa0173030:CDS
TCAGAAGAAGAAAGACGCCA+TGG 0.630309 1_4:-58217328 Msa0173030:CDS
CCCAACAGATGCAACTGTAA+CGG 0.630410 1_4:+58218897 None:intergenic
CGGCCGATCAAGGTTGCCAA+CGG 0.631386 1_4:+58219689 None:intergenic
TCCTAGCTTTCAAATGCTGG+CGG 0.631854 1_4:-58218440 Msa0173030:intron
CCGGTATCTAAGCCATGGGA+AGG 0.632107 1_4:-58217372 Msa0173030:CDS
TACAGAAATGTTGTATACAG+AGG 0.632924 1_4:+58220368 None:intergenic
GGCAACACAGGATAGTCTGA+AGG 0.635849 1_4:+58218145 None:intergenic
AAAGCGACAACTAGAGCCCG+AGG 0.640273 1_4:+58219083 None:intergenic
CAACGTATTCGGAGGCAATG+AGG 0.642352 1_4:-58218749 Msa0173030:intron
CGATGCATATTAGATACCCA+GGG 0.643134 1_4:-58218868 Msa0173030:CDS
ACTGGTAAACCATCACCAGA+TGG 0.656343 1_4:+58217417 None:intergenic
AAGTGTGTGTGTGACAAATG+AGG 0.666861 1_4:-58217166 Msa0173030:three_prime_UTR
GGAAGACTCAACGTATTCGG+AGG 0.678380 1_4:-58218757 Msa0173030:CDS
AACGATATACTCTCCCACAT+CGG 0.681964 1_4:+58218588 None:intergenic
AGAACTTGCTGCCAAGCTGG+TGG 0.683075 1_4:-58218336 Msa0173030:CDS
ACGGCTGCCAAGTCATGTCG+TGG 0.691102 1_4:-58218368 Msa0173030:CDS
TGTTGTATACAGAGGAAACG+CGG 0.694406 1_4:+58220376 None:intergenic
AAGGAATAATATCTACAACA+AGG 0.708500 1_4:+58218164 None:intergenic
CATACCGCAAGGTATAAGCA+GGG 0.741792 1_4:-58219126 Msa0173030:CDS
ACCGCAAGGTATAAGCAGGG+AGG 0.743248 1_4:-58219123 Msa0173030:CDS
GTTCTGCAATGCTTACAGAG+AGG 0.751165 1_4:-58217988 Msa0173030:intron
TGCATATTAGATACCCAGGG+AGG 0.753250 1_4:-58218865 Msa0173030:CDS
CTGCTAGGCAAACTGCACAG+CGG 0.759343 1_4:+58217445 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAATAATTGTTTTTTA+AGG + chr1_4:58216966-58216985 None:intergenic 10.0%
!! ATAAAAAGATAAATAAAATC+TGG + chr1_4:58217203-58217222 None:intergenic 10.0%
!!! ATAAATATATTGATTTTGTT+TGG - chr1_4:58217588-58217607 Msa0173030:intron 10.0%
!!! TTGAAAATGCATTTTATAAA+TGG - chr1_4:58219601-58219620 Msa0173030:CDS 15.0%
!!! TCAATTTTTACTTTTTACAT+CGG + chr1_4:58219786-58219805 None:intergenic 15.0%
!!! ATATTTGTATAATTTTTCCT+TGG - chr1_4:58220149-58220168 Msa0173030:intron 15.0%
!!! CTATGATTAATTTGATTCTA+TGG - chr1_4:58217404-58217423 Msa0173030:CDS 20.0%
!! ATAAGATCTTATGAATGAAT+TGG - chr1_4:58217986-58218005 Msa0173030:intron 20.0%
!! TAAGGAAGAAATTATATGAA+AGG + chr1_4:58218685-58218704 None:intergenic 20.0%
!! AAGAACTACTAAACTTTAAA+AGG + chr1_4:58219463-58219482 None:intergenic 20.0%
!!! CATTTGATTATGAATCTTTT+TGG + chr1_4:58219728-58219747 None:intergenic 20.0%
!!! TTTTTGTTTTCTTAAAATGC+AGG - chr1_4:58219874-58219893 Msa0173030:intron 20.0%
!!! TTAACATTACACTTTTTGTT+TGG - chr1_4:58220401-58220420 Msa0173030:five_prime_UTR 20.0%
!!! TATATTGATTTGATTGCTAA+CGG - chr1_4:58220432-58220451 Msa0173030:five_prime_UTR 20.0%
! AAATGAAGAAGAAAAACTCA+CGG + chr1_4:58217164-58217183 None:intergenic 25.0%
!! AGAATGTTGATTATTATAGC+TGG + chr1_4:58217349-58217368 None:intergenic 25.0%
! AATTTGATTCTATGGAAGTA+AGG - chr1_4:58217412-58217431 Msa0173030:CDS 25.0%
!! TAGATCTATTAAGAGTCTTT+TGG - chr1_4:58217545-58217564 Msa0173030:intron 25.0%
!!! TTGATTTTGTTTGGATGATT+AGG - chr1_4:58217597-58217616 Msa0173030:intron 25.0%
!!! GAAATGTTGACTTATAAACT+AGG - chr1_4:58217629-58217648 Msa0173030:intron 25.0%
! ATTGATATCATAACCATTGT+GGG + chr1_4:58218143-58218162 None:intergenic 25.0%
! TATTGATATCATAACCATTG+TGG + chr1_4:58218144-58218163 None:intergenic 25.0%
! CAATGGTTATGATATCAATA+AGG - chr1_4:58218144-58218163 Msa0173030:CDS 25.0%
! ATCAATAAGGAATTCTTGAT+TGG - chr1_4:58218157-58218176 Msa0173030:CDS 25.0%
!! AGTTTTCTTTGTATCCTTAT+TGG - chr1_4:58218365-58218384 Msa0173030:CDS 25.0%
!! TAAAATTTAATTTCACCTGC+AGG - chr1_4:58218416-58218435 Msa0173030:CDS 25.0%
!! AAAATTTAATTTCACCTGCA+GGG - chr1_4:58218417-58218436 Msa0173030:CDS 25.0%
!! TTTGGTTCCTTAATATGTTT+AGG - chr1_4:58218715-58218734 Msa0173030:intron 25.0%
! TTCCTTAATATGTTTAGGTA+GGG - chr1_4:58218720-58218739 Msa0173030:intron 25.0%
! AAGGAATAATATCTACAACA+AGG + chr1_4:58219222-58219241 None:intergenic 25.0%
! TGATTGGAACAAAAATGTTT+AGG - chr1_4:58219502-58219521 Msa0173030:intron 25.0%
! GATTGGAACAAAAATGTTTA+GGG - chr1_4:58219503-58219522 Msa0173030:intron 25.0%
!!! GGTTGTTGCAAATTATTTTT+TGG - chr1_4:58220275-58220294 Msa0173030:five_prime_UTR 25.0%
TACAGAAATGTTGTATACAG+AGG + chr1_4:58217018-58217037 None:intergenic 30.0%
!! TTTGCTCTTTGATTTTCGAT+TGG + chr1_4:58217041-58217060 None:intergenic 30.0%
AAGAGTTGAAAAACCCTAAT+GGG + chr1_4:58217093-58217112 None:intergenic 30.0%
!! CAGAATTTTGAGTATGAGAA+AGG + chr1_4:58217118-58217137 None:intergenic 30.0%
AATGAAGAAGAAAAACTCAC+GGG + chr1_4:58217163-58217182 None:intergenic 30.0%
AGTATCTTAGCTTAGTTAGT+TGG - chr1_4:58217272-58217291 Msa0173030:three_prime_UTR 30.0%
! TTTTGTACTTCTAGACTCAA+TGG - chr1_4:58217476-58217495 Msa0173030:CDS 30.0%
TGAGTAAGACTGAATTATCA+AGG - chr1_4:58217653-58217672 Msa0173030:intron 30.0%
TTAGTGAAATGTTTGATGAG+AGG - chr1_4:58217928-58217947 Msa0173030:intron 30.0%
!! TTTGATGAGAGGTATTTGAT+TGG - chr1_4:58217939-58217958 Msa0173030:intron 30.0%
TAAGAAAGATACATTCCCTT+TGG + chr1_4:58218034-58218053 None:intergenic 30.0%
AAAGGGAATGTATCTTTCTT+AGG - chr1_4:58218033-58218052 Msa0173030:CDS 30.0%
GACATGGAATTTCAGAAAAA+AGG - chr1_4:58218333-58218352 Msa0173030:CDS 30.0%
! AAATTTAATTTCACCTGCAG+GGG - chr1_4:58218418-58218437 Msa0173030:CDS 30.0%
!!! CTTCCTTATCTAACTTGTTT+TGG - chr1_4:58218697-58218716 Msa0173030:intron 30.0%
GAACCAAAACAAGTTAGATA+AGG + chr1_4:58218703-58218722 None:intergenic 30.0%
GTTCCTTAATATGTTTAGGT+AGG - chr1_4:58218719-58218738 Msa0173030:intron 30.0%
GTAAACACCTTCACATAAAA+GGG + chr1_4:58218885-58218904 None:intergenic 30.0%
AGTAAACACCTTCACATAAA+AGG + chr1_4:58218886-58218905 None:intergenic 30.0%
AGACTAATTATAGCTTCTGA+TGG - chr1_4:58218967-58218986 Msa0173030:intron 30.0%
TTATAGCTTCTGATGGAATT+TGG - chr1_4:58218974-58218993 Msa0173030:intron 30.0%
TATAGCTTCTGATGGAATTT+GGG - chr1_4:58218975-58218994 Msa0173030:intron 30.0%
GAATTCAAGTCTCATTATGT+TGG - chr1_4:58219118-58219137 Msa0173030:CDS 30.0%
!!! TCTATTGTTTTAACCTGAAC+AGG - chr1_4:58219156-58219175 Msa0173030:CDS 30.0%
AATAATATCTACAACAAGGC+AGG + chr1_4:58219218-58219237 None:intergenic 30.0%
AGCACATTATGTTTCTTTCT+TGG + chr1_4:58219277-58219296 None:intergenic 30.0%
!! AACTAGAGAAAGTGCATTTT+CGG + chr1_4:58219680-58219699 None:intergenic 30.0%
!!! ATGTCCTTATTTTCCCATTT+TGG - chr1_4:58219827-58219846 Msa0173030:intron 30.0%
!!! TGTCCTTATTTTCCCATTTT+GGG - chr1_4:58219828-58219847 Msa0173030:intron 30.0%
ATACCAGAATTTGTATTGGA+AGG + chr1_4:58219915-58219934 None:intergenic 30.0%
GTGAATACCAGAATTTGTAT+TGG + chr1_4:58219919-58219938 None:intergenic 30.0%
ATAACTATACTGTTGTTGCT+CGG + chr1_4:58220101-58220120 None:intergenic 30.0%
! TAATTTGCAACAACCTTCAA+TGG + chr1_4:58220270-58220289 None:intergenic 30.0%
GAAGAGTTGAAAAACCCTAA+TGG + chr1_4:58217094-58217113 None:intergenic 35.0%
ATGAGCACTATAGTGAAACA+TGG + chr1_4:58217443-58217462 None:intergenic 35.0%
! TTTTTCCATTTCGTAGCTCA+CGG + chr1_4:58217717-58217736 None:intergenic 35.0%
TGAGCTACGAAATGGAAAAA+CGG - chr1_4:58217717-58217736 Msa0173030:intron 35.0%
!! AACGTCATTTTCTGTTTTCG+CGG - chr1_4:58217840-58217859 Msa0173030:intron 35.0%
! ACATTTTCTCAGTGATCAAG+TGG - chr1_4:58218103-58218122 Msa0173030:CDS 35.0%
GCTACGTTTGTGATTATTGA+TGG - chr1_4:58218458-58218477 Msa0173030:intron 35.0%
CGTTTGTGATTATTGATGGA+TGG - chr1_4:58218462-58218481 Msa0173030:intron 35.0%
TGGAAGAAAATGTAGAAGAG+AGG - chr1_4:58218573-58218592 Msa0173030:CDS 35.0%
GGAAGAAAATGTAGAAGAGA+GGG - chr1_4:58218574-58218593 Msa0173030:CDS 35.0%
ACTTGCTTAACATGTGGTAT+TGG + chr1_4:58218821-58218840 None:intergenic 35.0%
!!! GTGAGTTGGTTTCATTTTCA+TGG - chr1_4:58218845-58218864 Msa0173030:CDS 35.0%
!! TTCTAAAGTTCAATGCTCAC+TGG - chr1_4:58218905-58218924 Msa0173030:CDS 35.0%
AAAGCTAGGAAACAAATCAG+GGG + chr1_4:58218933-58218952 None:intergenic 35.0%
GAAAGCTAGGAAACAAATCA+GGG + chr1_4:58218934-58218953 None:intergenic 35.0%
TGAAAGCTAGGAAACAAATC+AGG + chr1_4:58218935-58218954 None:intergenic 35.0%
GGATACTTTGTCTTCTGATA+CGG - chr1_4:58218996-58219015 Msa0173030:intron 35.0%
! GTTAAGGTGATGGTTATTTG+TGG - chr1_4:58219069-58219088 Msa0173030:CDS 35.0%
!!! GGCAGTGTTCTTATTTTTTG+AGG - chr1_4:58219096-58219115 Msa0173030:CDS 35.0%
TCTCAAAACTCGACAAACAA+AGG - chr1_4:58219322-58219341 Msa0173030:intron 35.0%
ACTAATAAGCTTTCTGCAGT+TGG - chr1_4:58219346-58219365 Msa0173030:intron 35.0%
! TTCCAATCACTTGTTGATGA+TGG + chr1_4:58219491-58219510 None:intergenic 35.0%
! TGTTTAGGGTCTGTTTACTT+CGG - chr1_4:58219517-58219536 Msa0173030:intron 35.0%
ACACCCAAAATGGGAAAATA+AGG + chr1_4:58219834-58219853 None:intergenic 35.0%
CACATGAATACACCCAAAAT+GGG + chr1_4:58219843-58219862 None:intergenic 35.0%
ACACATGAATACACCCAAAA+TGG + chr1_4:58219844-58219863 None:intergenic 35.0%
! GTTTTCTTAAAATGCAGGCT+AGG - chr1_4:58219879-58219898 Msa0173030:intron 35.0%
GTTCCTTCCAATACAAATTC+TGG - chr1_4:58219909-58219928 Msa0173030:intron 35.0%
! ATAAAATGATCGGTGTTCAC+TGG + chr1_4:58219987-58220006 None:intergenic 35.0%
TACCGGAGTGATAAAATGAT+CGG + chr1_4:58219997-58220016 None:intergenic 35.0%
!! CAATGCTTGGTTCTACATTT+GGG - chr1_4:58220181-58220200 Msa0173030:intron 35.0%
AGAATTGAGTTAGCTAGGTT+AGG - chr1_4:58220243-58220262 Msa0173030:five_prime_UTR 35.0%
TGTTGTATACAGAGGAAACG+CGG + chr1_4:58217010-58217029 None:intergenic 40.0%
TCTACGCATGTTGAGTGAAT+TGG + chr1_4:58217529-58217548 None:intergenic 40.0%
! GAGAAGCCTTTTGTGAAGTA+TGG - chr1_4:58217739-58217758 Msa0173030:intron 40.0%
! TCATTTTCTGTTTTCGCGGT+AGG - chr1_4:58217844-58217863 Msa0173030:intron 40.0%
GCGTCAATTTATTCCCACAA+TGG - chr1_4:58218127-58218146 Msa0173030:CDS 40.0%
ATATACCATTATGCCCGTCA+AGG + chr1_4:58218188-58218207 None:intergenic 40.0%
TTCCTTCGCAAAAATAGCAG+CGG + chr1_4:58218211-58218230 None:intergenic 40.0%
! CGCTTTTGTGAAAACTGACA+TGG - chr1_4:58218317-58218336 Msa0173030:intron 40.0%
GAACAAGTACAGCTCCAATA+AGG + chr1_4:58218382-58218401 None:intergenic 40.0%
CTGGGTATCTAATATGCATC+GGG + chr1_4:58218516-58218535 None:intergenic 40.0%
CCTGGGTATCTAATATGCAT+CGG + chr1_4:58218517-58218536 None:intergenic 40.0%
CGATGCATATTAGATACCCA+GGG - chr1_4:58218515-58218534 Msa0173030:intron 40.0%
AAAATGTAGAAGAGAGGGAG+CGG - chr1_4:58218579-58218598 Msa0173030:CDS 40.0%
GTAGGAAGACTCAACGTATT+CGG - chr1_4:58218623-58218642 Msa0173030:CDS 40.0%
GGCCCTACCTAAACATATTA+AGG + chr1_4:58218725-58218744 None:intergenic 40.0%
TTGTGCCTTTCTAGATCGAT+TGG - chr1_4:58218767-58218786 Msa0173030:CDS 40.0%
TGTCACCAATCGATCTAGAA+AGG + chr1_4:58218775-58218794 None:intergenic 40.0%
AACGATATACTCTCCCACAT+CGG + chr1_4:58218798-58218817 None:intergenic 40.0%
ACCACATGTTAAGCAAGTGA+AGG - chr1_4:58218823-58218842 Msa0173030:CDS 40.0%
ACCTTCACTTGCTTAACATG+TGG + chr1_4:58218827-58218846 None:intergenic 40.0%
TTAAGCAAGTGAAGGTGAGT+TGG - chr1_4:58218831-58218850 Msa0173030:CDS 40.0%
! CTAGTGACCCTTTTATGTGA+AGG - chr1_4:58218875-58218894 Msa0173030:CDS 40.0%
! GTTTCCTAGCTTTCAAATGC+TGG - chr1_4:58218940-58218959 Msa0173030:CDS 40.0%
TGTTTCTTTCTTGGAGTTGC+TGG + chr1_4:58219268-58219287 None:intergenic 40.0%
GTGCTTAGTTCACTTCTCTT+TGG - chr1_4:58219292-58219311 Msa0173030:intron 40.0%
TGTGGAGGAGTTATTTGAAG+AGG - chr1_4:58219372-58219391 Msa0173030:intron 40.0%
GTGGAGGAGTTATTTGAAGA+GGG - chr1_4:58219373-58219392 Msa0173030:intron 40.0%
TGCCATCATCAACAAGTGAT+TGG - chr1_4:58219486-58219505 Msa0173030:intron 40.0%
! GTTTAGGGTCTGTTTACTTC+GGG - chr1_4:58219518-58219537 Msa0173030:intron 40.0%
CTGTCATTGAGAGAACATGA+AGG + chr1_4:58219705-58219724 None:intergenic 40.0%
!!! TTACTTTTTACATCGGCAGC+TGG + chr1_4:58219779-58219798 None:intergenic 40.0%
CTTAAAATGCAGGCTAGGTA+AGG - chr1_4:58219884-58219903 Msa0173030:intron 40.0%
! CACCGATCATTTTATCACTC+CGG - chr1_4:58219992-58220011 Msa0173030:intron 40.0%
!! GCAATGCTTGGTTCTACATT+TGG - chr1_4:58220180-58220199 Msa0173030:intron 40.0%
AAGTGTGTGTGTGACAAATG+AGG - chr1_4:58220217-58220236 Msa0173030:intron 40.0%
GGTTGAGAATTGAGTTAGCT+AGG - chr1_4:58220238-58220257 Msa0173030:intron 40.0%
TGTGCTCTCAACACCCATTA+GGG - chr1_4:58217077-58217096 Msa0173030:three_prime_UTR 45.0%
! GCCTTTTGTGAAGTATGGAC+AGG - chr1_4:58217744-58217763 Msa0173030:intron 45.0%
GCCTGTCCATACTTCACAAA+AGG + chr1_4:58217748-58217767 None:intergenic 45.0%
TTTGTGAAGTATGGACAGGC+TGG - chr1_4:58217748-58217767 Msa0173030:intron 45.0%
!! CAGGCTGGTTTGGCAAAAAA+AGG - chr1_4:58217763-58217782 Msa0173030:intron 45.0%
ACAGATTGCCATAGAGTTCC+TGG - chr1_4:58217808-58217827 Msa0173030:intron 45.0%
AAGAATCGCCAGGAACTCTA+TGG + chr1_4:58217819-58217838 None:intergenic 45.0%
AATGACGTTGAAGAATCGCC+AGG + chr1_4:58217829-58217848 None:intergenic 45.0%
TTCTGCTTCGAGAGACCAAA+GGG - chr1_4:58218016-58218035 Msa0173030:CDS 45.0%
CTTGATTGGCAGATCCTTGA+CGG - chr1_4:58218171-58218190 Msa0173030:CDS 45.0%
TTGATTGGCAGATCCTTGAC+GGG - chr1_4:58218172-58218191 Msa0173030:CDS 45.0%
!!! ATCCGCTGCTATTTTTGCGA+AGG - chr1_4:58218206-58218225 Msa0173030:CDS 45.0%
CATACCGCAAGGTATAAGCA+GGG - chr1_4:58218257-58218276 Msa0173030:intron 45.0%
AAGGTATAAGCAGGGAGGAA+TGG - chr1_4:58218265-58218284 Msa0173030:intron 45.0%
ACCGTTACAGTTGCATCTGT+TGG - chr1_4:58218485-58218504 Msa0173030:intron 45.0%
CCCAACAGATGCAACTGTAA+CGG + chr1_4:58218489-58218508 None:intergenic 45.0%
CCGTTACAGTTGCATCTGTT+GGG - chr1_4:58218486-58218505 Msa0173030:intron 45.0%
CGTTACAGTTGCATCTGTTG+GGG - chr1_4:58218487-58218506 Msa0173030:intron 45.0%
CCGATGCATATTAGATACCC+AGG - chr1_4:58218514-58218533 Msa0173030:intron 45.0%
TGCATATTAGATACCCAGGG+AGG - chr1_4:58218518-58218537 Msa0173030:intron 45.0%
CTTGACAGTTGATCACAGAC+TGG - chr1_4:58218553-58218572 Msa0173030:intron 45.0%
AAATGTAGAAGAGAGGGAGC+GGG - chr1_4:58218580-58218599 Msa0173030:CDS 45.0%
! TCCTAGCTTTCAAATGCTGG+CGG - chr1_4:58218943-58218962 Msa0173030:CDS 45.0%
GTGATGGTTATTTGTGGCAG+TGG - chr1_4:58219075-58219094 Msa0173030:CDS 45.0%
AACCTGAACAGGAAGCTCTA+AGG - chr1_4:58219167-58219186 Msa0173030:CDS 45.0%
!! AGGAAGCTCTAAGGTCAAGA+GGG - chr1_4:58219176-58219195 Msa0173030:CDS 45.0%
TTTCTTGGAGTTGCTGGCAT+CGG + chr1_4:58219262-58219281 None:intergenic 45.0%
! GCTTTCTGCAGTTGGTATTG+TGG - chr1_4:58219354-58219373 Msa0173030:intron 45.0%
! TTCTGCAGTTGGTATTGTGG+AGG - chr1_4:58219357-58219376 Msa0173030:intron 45.0%
GTTCTGCAATGCTTACAGAG+AGG - chr1_4:58219395-58219414 Msa0173030:intron 45.0%
ACTGGTAAACCATCACCAGA+TGG + chr1_4:58219969-58219988 None:intergenic 45.0%
TGACAGTTTGTGCAGAGGAA+TGG + chr1_4:58220038-58220057 None:intergenic 45.0%
TCTTCTGACAGTTTGTGCAG+AGG + chr1_4:58220043-58220062 None:intergenic 45.0%
TCAGAAGAAGAAAGACGCCA+TGG - chr1_4:58220055-58220074 Msa0173030:intron 45.0%
AAGAAGAAAGACGCCATGGA+AGG - chr1_4:58220059-58220078 Msa0173030:intron 45.0%
AAGACGCCATGGAAGGAAAA+AGG - chr1_4:58220066-58220085 Msa0173030:intron 45.0%
! TCAGGACCTTTTTCCTTCCA+TGG + chr1_4:58220075-58220094 None:intergenic 45.0%
!! TGCTTGGTTCTACATTTGGG+TGG - chr1_4:58220184-58220203 Msa0173030:intron 45.0%
AGCTAGGTTAGGTCCATTGA+AGG - chr1_4:58220254-58220273 Msa0173030:five_prime_UTR 45.0%
CTGTGCTCTCAACACCCATT+AGG - chr1_4:58217076-58217095 Msa0173030:three_prime_UTR 50.0%
GAAGCAACCACTTGTTCCGT+TGG - chr1_4:58217678-58217697 Msa0173030:intron 50.0%
AAGGTTGCCAACGGAACAAG+TGG + chr1_4:58217688-58217707 None:intergenic 50.0%
GTTCCGTTGGCAACCTTGAT+CGG - chr1_4:58217691-58217710 Msa0173030:intron 50.0%
! GAAGTATGGACAGGCTGGTT+TGG - chr1_4:58217753-58217772 Msa0173030:intron 50.0%
GTTCTGCTTCGAGAGACCAA+AGG - chr1_4:58218015-58218034 Msa0173030:CDS 50.0%
! CAGATCCTTGACGGGCATAA+TGG - chr1_4:58218180-58218199 Msa0173030:CDS 50.0%
CCTGCTTATACCTTGCGGTA+TGG + chr1_4:58218259-58218278 None:intergenic 50.0%
CCATACCGCAAGGTATAAGC+AGG - chr1_4:58218256-58218275 Msa0173030:intron 50.0%
TCCTCCCTGCTTATACCTTG+CGG + chr1_4:58218264-58218283 None:intergenic 50.0%
! AATGGCTTCAAGCACTGCCT+CGG - chr1_4:58218283-58218302 Msa0173030:intron 50.0%
GAGAGAAACAACACCTCCCT+GGG + chr1_4:58218534-58218553 None:intergenic 50.0%
AGAGAGAAACAACACCTCCC+TGG + chr1_4:58218535-58218554 None:intergenic 50.0%
TCTTCCTACTTCACCACCAC+TGG + chr1_4:58218612-58218631 None:intergenic 50.0%
GGAAGACTCAACGTATTCGG+AGG - chr1_4:58218626-58218645 Msa0173030:CDS 50.0%
CAACGTATTCGGAGGCAATG+AGG - chr1_4:58218634-58218653 Msa0173030:CDS 50.0%
! CTAGAAAGGCACAATCCACC+AGG + chr1_4:58218761-58218780 None:intergenic 50.0%
! ATCGATTGGTGACACCGATG+TGG - chr1_4:58218781-58218800 Msa0173030:CDS 50.0%
! TCGATTGGTGACACCGATGT+GGG - chr1_4:58218782-58218801 Msa0173030:CDS 50.0%
TCCGCCAGCATTTGAAAGCT+AGG + chr1_4:58218947-58218966 None:intergenic 50.0%
AGGTACACCACGACATGACT+TGG + chr1_4:58219025-58219044 None:intergenic 50.0%
CATCACCTTAACCACCAGCT+TGG + chr1_4:58219061-58219080 None:intergenic 50.0%
CAAGCTGGTGGTTAAGGTGA+TGG - chr1_4:58219059-58219078 Msa0173030:CDS 50.0%
GACCTTAGAGCTTCCTGTTC+AGG + chr1_4:58219172-58219191 None:intergenic 50.0%
!! CAGGAAGCTCTAAGGTCAAG+AGG - chr1_4:58219175-58219194 Msa0173030:CDS 50.0%
! TCTAAGGTCAAGAGGGCTGA+AGG - chr1_4:58219183-58219202 Msa0173030:CDS 50.0%
GGCAACACAGGATAGTCTGA+AGG + chr1_4:58219241-58219260 None:intergenic 50.0%
TGCCTAGCAGATCAACCATC+TGG - chr1_4:58219951-58219970 Msa0173030:intron 50.0%
CACCAGATGGTTGATCTGCT+AGG + chr1_4:58219956-58219975 None:intergenic 50.0%
GCAGATCAACCATCTGGTGA+TGG - chr1_4:58219957-58219976 Msa0173030:intron 50.0%
TCACTCCGGTATCTAAGCCA+TGG - chr1_4:58220006-58220025 Msa0173030:intron 50.0%
CACTCCGGTATCTAAGCCAT+GGG - chr1_4:58220007-58220026 Msa0173030:intron 50.0%
CCTTCCCATGGCTTAGATAC+CGG + chr1_4:58220014-58220033 None:intergenic 50.0%
ACTGTTGTTGCTCGGACTTC+AGG + chr1_4:58220093-58220112 None:intergenic 50.0%
! AAGCATTGCTCACACTGCCA+AGG + chr1_4:58220169-58220188 None:intergenic 50.0%
TTGGCAGTGTGAGCAATGCT+TGG - chr1_4:58220168-58220187 Msa0173030:intron 50.0%
!! TTTGGCTTGTGCTCAGCTGT+TGG - chr1_4:58220293-58220312 Msa0173030:five_prime_UTR 50.0%
ATCGGCCGTGAGCTACGAAA+TGG - chr1_4:58217709-58217728 Msa0173030:intron 55.0%
GTCATGAGTGCCATACCGCA+AGG - chr1_4:58218246-58218265 Msa0173030:intron 55.0%
ACCGCAAGGTATAAGCAGGG+AGG - chr1_4:58218260-58218279 Msa0173030:intron 55.0%
! ATGGCTTCAAGCACTGCCTC+GGG - chr1_4:58218284-58218303 Msa0173030:intron 55.0%
AAAGCGACAACTAGAGCCCG+AGG + chr1_4:58218303-58218322 None:intergenic 55.0%
TCACCTGCAGGGGAAACTTC+TGG - chr1_4:58218428-58218447 Msa0173030:CDS 55.0%
GTTCCAGAAGTTTCCCCTGC+AGG + chr1_4:58218434-58218453 None:intergenic 55.0%
! ACAGCCAGTGGTGGTGAAGT+AGG - chr1_4:58218605-58218624 Msa0173030:CDS 55.0%
AGCTTGGCAGCAAGTTCTGC+AGG + chr1_4:58219045-58219064 None:intergenic 55.0%
!! TGCAGAACTTGCTGCCAAGC+TGG - chr1_4:58219044-58219063 Msa0173030:intron 55.0%
!! AGAACTTGCTGCCAAGCTGG+TGG - chr1_4:58219047-58219066 Msa0173030:intron 55.0%
TGCTGCCAAGCTGGTGGTTA+AGG - chr1_4:58219053-58219072 Msa0173030:CDS 55.0%
CTGCTAGGCAAACTGCACAG+CGG + chr1_4:58219941-58219960 None:intergenic 55.0%
CCGGTATCTAAGCCATGGGA+AGG - chr1_4:58220011-58220030 Msa0173030:intron 55.0%
CAGAGGAATGGACCTTCCCA+TGG + chr1_4:58220026-58220045 None:intergenic 55.0%
CGGCCGATCAAGGTTGCCAA+CGG + chr1_4:58217697-58217716 None:intergenic 60.0%
TCGTAGCTCACGGCCGATCA+AGG + chr1_4:58217707-58217726 None:intergenic 60.0%
AGGTAGGGCCTCTTCGCTGT+TGG - chr1_4:58218735-58218754 Msa0173030:intron 60.0%
ACGGCTGCCAAGTCATGTCG+TGG - chr1_4:58219015-58219034 Msa0173030:intron 60.0%
!! GTTGCTGGCATCGGCAACAC+AGG + chr1_4:58219253-58219272 None:intergenic 60.0%
!! AGGGAGCGGGTTACAGCCAG+TGG - chr1_4:58218593-58218612 Msa0173030:CDS 65.0%
!! GAGCGGGTTACAGCCAGTGG+TGG - chr1_4:58218596-58218615 Msa0173030:CDS 65.0%
CCACCAGGCCAACAGCGAAG+AGG + chr1_4:58218746-58218765 None:intergenic 65.0%
! CCTCTTCGCTGTTGGCCTGG+TGG - chr1_4:58218743-58218762 Msa0173030:intron 65.0%
GGGCCTCTTCGCTGTTGGCC+TGG - chr1_4:58218740-58218759 Msa0173030:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 58216950 58220455 58216950 ID=Msa0173030;Name=Msa0173030
chr1_4 mRNA 58216950 58220455 58216950 ID=Msa0173030-mRNA-1;Parent=Msa0173030;Name=Msa0173030-mRNA-1;_AED=0.09;_eAED=0.09;_QI=246|1|1|1|0.85|0.75|8|361|423
chr1_4 exon 58216950 58217509 58216950 ID=Msa0173030-mRNA-1:exon:17982;Parent=Msa0173030-mRNA-1
chr1_4 exon 58217989 58218227 58217989 ID=Msa0173030-mRNA-1:exon:17981;Parent=Msa0173030-mRNA-1
chr1_4 exon 58218331 58218456 58218331 ID=Msa0173030-mRNA-1:exon:17980;Parent=Msa0173030-mRNA-1
chr1_4 exon 58218561 58218668 58218561 ID=Msa0173030-mRNA-1:exon:17979;Parent=Msa0173030-mRNA-1
chr1_4 exon 58218750 58218967 58218750 ID=Msa0173030-mRNA-1:exon:17978;Parent=Msa0173030-mRNA-1
chr1_4 exon 58219051 58219222 58219051 ID=Msa0173030-mRNA-1:exon:17977;Parent=Msa0173030-mRNA-1
chr1_4 exon 58219544 58219786 58219544 ID=Msa0173030-mRNA-1:exon:17976;Parent=Msa0173030-mRNA-1
chr1_4 exon 58220243 58220455 58220243 ID=Msa0173030-mRNA-1:exon:17975;Parent=Msa0173030-mRNA-1
chr1_4 five_prime_UTR 58220243 58220455 58220243 ID=Msa0173030-mRNA-1:five_prime_utr;Parent=Msa0173030-mRNA-1
chr1_4 five_prime_UTR 58219754 58219786 58219754 ID=Msa0173030-mRNA-1:five_prime_utr;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58219544 58219753 58219544 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58219051 58219222 58219051 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58218750 58218967 58218750 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58218561 58218668 58218561 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58218331 58218456 58218331 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58217989 58218227 58217989 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 CDS 58217311 58217509 58217311 ID=Msa0173030-mRNA-1:cds;Parent=Msa0173030-mRNA-1
chr1_4 three_prime_UTR 58216950 58217310 58216950 ID=Msa0173030-mRNA-1:three_prime_utr;Parent=Msa0173030-mRNA-1
Gene Sequence

>Msa0173030

ATGAGTAAGACTGAATTATCAAGGATGAAGCAACCACTTGTTCCGTTGGCAACCTTGATCGGCCGTGAGCTACGAAATGGAAAAACGGAGAAGCCTTTTGTGAAGTATGGACAGGCTGGTTTGGCAAAAAAAGGAGAAGATTACTTTCTAATCAAGACAGATTGCCATAGAGTTCCTGGCGATTCTTCAACGTCATTTTCTGTTTTCGCGATCCTTGACGGGCATAATGGTATATCCGCTGCTATTTTTGCGAAGGAAAACATAATAAACAATGTCATGAGTGCCATACCGCAAGGTATAAGCAGGGAGGAATGGCTTCAAGCACTGCCTCGGGCTCTAGTTGTCGCTTTTGTGAAAACTGACATGGAATTTCAGAAAAAAGGGGAAACTTCTGGAACGACAGCTACGTTTGTGATTATTGATGGATGGACCGTTACAGTTGCATCTGTTGGGGATTCCCGATGCATATTAGATACCCAGGGAGGTGTTGTTTCTCTCTTGACAGTTGATCACAGACTGGAAGAAAATGTAGAAGAGAGGGAGCGGGTTACAGCCAGTGGTGGTGAAGTAGGAAGACTCAACGTATTCGGAGGCAATGAGGTAGGGCCTCTTCGCTGTTGGCCTGGTGGATTGTGCCTTTCTAGATCGATTGGTGACACCGATGTGGGAGAGTATATCGTTCCAATACCACATGTTAAGCAAGTGAAGCTTTCAAATGCTGGCGGAAGACTAATTATAGCTTCTGATGGAATTTGGGATACTTTGTCTTCTGATACGGCTGCCAAGTCATGTCGTGGTGTACCTGCAGAACTTGCTGCCAAGCTGGTGGTTAAGGAAGCTCTAAGGTCAAGAGGGCTGAAGGATGATACAACCTGCCTTGTTGTAGATATTATTCCTTCAGACTATCCTGTGTTGCCGATGCCAGCAACTCCAAGAAAGAAACATAATGTGCTTAGTTCACTTCTCTTTGGAAAGAAATCTCAAAACTCGACAAACAAAGGAACTAATAAGCTTTCTGCAGTTGGTATTGTGGAGGAGTTATTTGAAGAGGGTTCTGCAATGCTTACAGAGAGGCTAGGTAAGGATGTTCCTTCCAATACAAATTCTGGTATTCACCGCTGTGCAGTTTGCCTAGCAGATCAACCATCTGGTGATGGTTTACCAGTGAACACCGATCATTTTATCACTCCGGTATCTAAGCCATGGGAAGGTCCATTCCTCTGCACAAACTGTCAGAAGAAGAAAGACGCCATGGAAGGAAAAAGGTCCTGA
Protein sequence

>Msa0173030

MSKTELSRMKQPLVPLATLIGRELRNGKTEKPFVKYGQAGLAKKGEDYFLIKTDCHRVPGDSSTSFSVFAILDGHNGISAAIFAKENIINNVMSAIPQGISREEWLQALPRALVVAFVKTDMEFQKKGETSGTTATFVIIDGWTVTVASVGDSRCILDTQGGVVSLLTVDHRLEENVEERERVTASGGEVGRLNVFGGNEVGPLRCWPGGLCLSRSIGDTDVGEYIVPIPHVKQVKLSNAGGRLIIASDGIWDTLSSDTAAKSCRGVPAELAAKLVVKEALRSRGLKDDTTCLVVDIIPSDYPVLPMPATPRKKHNVLSSLLFGKKSQNSTNKGTNKLSAVGIVEELFEEGSAMLTERLGKDVPSNTNSGIHRCAVCLADQPSGDGLPVNTDHFITPVSKPWEGPFLCTNCQKKKDAMEGKRS*