AlfalfaGEDB | Msa0500660

M. sativa cultivar zhongmu-4 / Msa0500660

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
PFAM: PF00481.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500660	XP_003603027.1	98.341	422	7	0	14	435	1	422	0.0	857

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500660	sp\|Q9FX08\|P2C12_ARATH	76.168	428	100	1	14	441	1	426	0.0	698

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500660	G7J6C1	98.341	422	7	0	14	435	1	422	0.0	857

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0044510	Msa0500660	0.840268	9.631249e-58	-8.615850e-47
Msa0131610	Msa0500660	0.802143	6.453013e-49	-8.615850e-47
Msa0138090	Msa0500660	0.871596	6.059310e-67	-8.615850e-47
Msa0172320	Msa0500660	0.894285	2.848305e-75	-8.615850e-47
Msa0173030	Msa0500660	0.817292	3.557123e-52	-8.615850e-47
Msa0336700	Msa0500660	0.813284	2.766607e-51	-8.615850e-47
Msa0429980	Msa0500660	0.990868	1.010976e-184	-8.615850e-47
Msa0467570	Msa0500660	0.804765	1.845880e-49	-8.615850e-47
Msa0500640	Msa0500660	0.983535	5.215488e-158	-8.615850e-47
Msa0500660	Msa0526650	0.889651	2.000767e-73	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500660	MtrunA17_Chr3g0133801	98.341	422	7	0	14	435	1	422	0.0	857
Msa0500660	MtrunA17_Chr1g0153671	84.286	420	62	3	14	432	1	417	0.0	733
Msa0500660	MtrunA17_Chr1g0209451	58.824	425	167	5	16	436	6	426	1.03e-180	512
Msa0500660	MtrunA17_Chr7g0271971	57.279	419	176	2	17	435	8	423	1.38e-167	478
Msa0500660	MtrunA17_Chr1g0192471	55.961	411	178	2	23	432	14	422	2.44e-159	457
Msa0500660	MtrunA17_Chr7g0225501	53.630	427	162	16	23	432	32	439	2.66e-125	371
Msa0500660	MtrunA17_Chr2g0326251	48.357	213	94	8	120	326	7	209	1.21e-50	171
Msa0500660	MtrunA17_Chr1g0150451	30.738	244	145	8	79	317	108	332	4.26e-25	105
Msa0500660	MtrunA17_Chr3g0136711	28.514	249	154	8	79	322	65	294	2.54e-23	99.8
Msa0500660	MtrunA17_Chr1g0212961	32.766	235	134	11	80	311	127	340	3.30e-22	97.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500660	AT1G47380.1	76.168	428	100	1	14	441	1	426	0.0	698
Msa0500660	AT1G68410.4	56.355	417	177	2	23	436	19	433	1.10e-173	494
Msa0500660	AT1G68410.3	56.355	417	177	2	23	436	19	433	1.10e-173	494
Msa0500660	AT1G68410.1	56.355	417	177	2	23	436	19	433	1.10e-173	494
Msa0500660	AT1G68410.2	56.355	417	177	2	23	436	19	433	1.10e-173	494
Msa0500660	AT1G09160.2	56.532	421	180	2	15	435	6	423	6.98e-165	472
Msa0500660	AT1G09160.1	56.532	421	180	2	15	435	6	423	6.98e-165	472
Msa0500660	AT4G26080.1	30.545	275	146	8	72	313	165	427	2.25e-23	102
Msa0500660	AT5G57050.2	31.559	263	134	9	80	308	121	371	2.64e-23	101
Msa0500660	AT5G53140.1	29.856	278	160	9	57	324	115	367	2.83e-23	102

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Find 157 sgRNAs with CRISPR-Local

Find 341 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACGCATAGTTTGTTGT+TGG	0.438079	3_4:-41196225	None:intergenic
AAAGGTCCATCCCAGGGACG+CGG	0.595744	3_4:-41195438	None:intergenic
AAATACAGGCGGAGGTGCAG+AGG	0.629751	3_4:+41193670	Msa0500660:CDS
AAATCTTCTGATTGTTCATA+TGG	0.282600	3_4:+41195768	Msa0500660:exon
AACAAGTCTTCCTCCAGCAT+TGG	0.488721	3_4:-41194770	None:intergenic
AACATACGAAGATGACTCAG+AGG	0.704568	3_4:-41195143	None:intergenic
AACCACCCAAGAGTGGCGAA+AGG	0.663603	3_4:+41195878	Msa0500660:three_prime_UTR
AACCTTTCGCCACTCTTGGG+TGG	0.561700	3_4:-41195880	None:intergenic
AACTTGAACATGTTGCAGAG+TGG	0.681139	3_4:-41195333	None:intergenic
AAGAAGAAAGAAGCCATGGA+AGG	0.562499	3_4:+41195480	Msa0500660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTGTGTTTGAAGAAATTG+AGG	+	chr3_4:41191503-41191522	Msa0500660:CDS	30.0%
GTTGTGTTTGAAGAAATTGA+GGG	+	chr3_4:41191504-41191523	Msa0500660:CDS	30.0%
GCAAAGTCAACCAAAAAAAA+AGG	-	chr3_4:41192255-41192274	None:intergenic	30.0%
AAACAAAACCATTGCTTTGA+GGG	-	chr3_4:41192437-41192456	None:intergenic	30.0%
AAAACAAAACCATTGCTTTG+AGG	-	chr3_4:41192438-41192457	None:intergenic	30.0%
TCTTGCCTCCATTAAATATT+TGG	+	chr3_4:41192811-41192830	Msa0500660:intron	30.0%
ATTCATATGAAGATTCCAGA+TGG	-	chr3_4:41192859-41192878	None:intergenic	30.0%
AGAACAATGAGACAAAAAAC+AGG	+	chr3_4:41192939-41192958	Msa0500660:intron	30.0%
ACTAACTCTACTATAGGTAT+GGG	-	chr3_4:41193178-41193197	None:intergenic	30.0%
AACTAACTCTACTATAGGTA+TGG	-	chr3_4:41193179-41193198	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	41191217	41196281	41191217	ID=Msa0500660;Name=Msa0500660
chr3_4	mRNA	41191217	41196281	41191217	ID=Msa0500660-mRNA-1;Parent=Msa0500660;Name=Msa0500660-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0.88\|0.8\|1\|0.88\|0.9\|10\|513\|464
chr3_4	exon	41191217	41191223	41191217	ID=Msa0500660-mRNA-1:exon:8561;Parent=Msa0500660-mRNA-1
chr3_4	exon	41191499	41191734	41191499	ID=Msa0500660-mRNA-1:exon:8562;Parent=Msa0500660-mRNA-1
chr3_4	exon	41192614	41192785	41192614	ID=Msa0500660-mRNA-1:exon:8563;Parent=Msa0500660-mRNA-1
chr3_4	exon	41193381	41193534	41193381	ID=Msa0500660-mRNA-1:exon:8564;Parent=Msa0500660-mRNA-1
chr3_4	exon	41193628	41193691	41193628	ID=Msa0500660-mRNA-1:exon:8565;Parent=Msa0500660-mRNA-1
chr3_4	exon	41194180	41194287	41194180	ID=Msa0500660-mRNA-1:exon:8566;Parent=Msa0500660-mRNA-1
chr3_4	exon	41194766	41194891	41194766	ID=Msa0500660-mRNA-1:exon:8567;Parent=Msa0500660-mRNA-1
chr3_4	exon	41195001	41195236	41195001	ID=Msa0500660-mRNA-1:exon:8568;Parent=Msa0500660-mRNA-1
chr3_4	exon	41195317	41195516	41195317	ID=Msa0500660-mRNA-1:exon:8569;Parent=Msa0500660-mRNA-1
chr3_4	exon	41195677	41196281	41195677	ID=Msa0500660-mRNA-1:exon:8570;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41191217	41191223	41191217	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41191499	41191734	41191499	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41192614	41192785	41192614	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41193381	41193534	41193381	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41193628	41193691	41193628	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41194180	41194287	41194180	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41194766	41194891	41194766	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41195001	41195236	41195001	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41195317	41195516	41195317	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	CDS	41195677	41195768	41195677	ID=Msa0500660-mRNA-1:cds;Parent=Msa0500660-mRNA-1
chr3_4	three_prime_UTR	41195769	41196281	41195769	ID=Msa0500660-mRNA-1:three_prime_utr;Parent=Msa0500660-mRNA-1

Gene Sequence

>Msa0500660

ATGAATGGTGTTGTTGTGTTTGAAGAAATTGAGGGAACAATGTCTGGTCGGGGAGAACATCATTCGGTTCCCCTTTCGGTGCTGTTGAAGCGTGAATCGTTGAGCGAGAAAATCGAGAAACCTGATATGGTTGTTGTTTATGGAAATGCTAGCGAGAATAAGAAAGGAGAAGATTTCATTTTACTAAAGACCGAATGTCAAAGAGTCGTTTGTGATGGTGTTTCTACATATTCTGTTTTTGGGCTATTTGACGGACACAATGGATCTGCTGCTGCAATTTATGCCAAGGAGAATCTTCTGAACAATGTTTTAGGTGCCATTCCTCCTGATCTCAACAGAGATGAGTGGGTAGCAGCGTTGCCAAGGGCTTTGGTTGCTGGCTTTGTGAAGACTGATAAAGATTTTCAACAGAAAGCACAAACATCGGGAACAACTGTAACCTTCGTGATTATAGAAGGATGGGTTGTAACAGTTGCATCAGTTGGTGATTCCCGTTGCATACTTGAACCTTCTGAGGGCGGGCTTCATTACTTGTCAGCAGATCATCGACTGGACACAAATGAAGAAGAGAGGGTGCGCATCACTTCTAGTGGGGGTGAAGTTGGTCGGCTAAATACAGGCGGAGGTGCAGAGGTTGGTCCGTTGAGATGTTGGCCTGGTGGCTTGTGTCTCTCTCGATCCATTGGTGATATGGATGTTGGTGAATTTATTGTCCCTGTACCATATGTAAAGCAAGTGAAGCTTTCCAATGCTGGAGGAAGACTTGTTATCAGCAGTGATGGAGTCTGGGATGCTCTAACTGCAGAAATGGTCCTTGATTGTTGTCGTGGCATGTCGGCGGAGGCTGCAGCACCACATATTGTAAAAGAATCTTTGCAAGCAAAGGGACTTAGAGATGACACAACCTGCATCGTGGTCGATATATTACCCCAGGAGAAACCACCTACTTCTGCACCAACACAAAAGAAACCGGTAAAAGGAATGTTGAAGGCCATTTTCCGCAAAAAGTCCTCTGAGTCATCTTCGTATGTTGAAAAAGAATACGTTGAGCCAGATATGGTACGGGAACTATACGAGGAAGGATCTGCCTTGCTTTCAGAAAGGTTTGAAACAAAATATCCACTCTGCAACATGTTCAAGTTGTTCATGTGTGCAGTGTGTCAAGTAGAGATTAAACCTGGGGAGGGTATTTCAGTACATGAGGGTGCGCCTAACGTAAGGAAACCGCGTCCCTGGGATGGACCTTTCCTTTGCTTAAGTTGCCAAAAGAAGAAAGAAGCCATGGAAGGGAAACGGATATCAGAGATTGTTCATATAACGCAAAAGTCAAACTTCCAAGCAACTGTGACTCCCATTATTACATCGGGGGTTTCTCAGAATAAGGATTCATTTTGA

Protein sequence

>Msa0500660

MNGVVVFEEIEGTMSGRGEHHSVPLSVLLKRESLSEKIEKPDMVVVYGNASENKKGEDFILLKTECQRVVCDGVSTYSVFGLFDGHNGSAAAIYAKENLLNNVLGAIPPDLNRDEWVAALPRALVAGFVKTDKDFQQKAQTSGTTVTFVIIEGWVVTVASVGDSRCILEPSEGGLHYLSADHRLDTNEEERVRITSSGGEVGRLNTGGGAEVGPLRCWPGGLCLSRSIGDMDVGEFIVPVPYVKQVKLSNAGGRLVISSDGVWDALTAEMVLDCCRGMSAEAAAPHIVKESLQAKGLRDDTTCIVVDILPQEKPPTSAPTQKKPVKGMLKAIFRKKSSESSSYVEKEYVEPDMVRELYEEGSALLSERFETKYPLCNMFKLFMCAVCQVEIKPGEGISVHEGAPNVRKPRPWDGPFLCLSCQKKKEAMEGKRISEIVHITQKSNFQATVTPIITSGVSQNKDSF*