Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0085360 | Msa0132750 | -0.807103 | 5.952444e-50 | -8.615850e-47 |
| Msa0132750 | Msa0229490 | -0.807971 | 3.895004e-50 | -8.615850e-47 |
| Msa0132750 | Msa0909000 | -0.804745 | 1.863871e-49 | -8.615850e-47 |
| Msa0132750 | Msa1046020 | 0.803965 | 2.709563e-49 | -8.615850e-47 |
| Msa0132750 | Msa1046060 | 0.807148 | 5.822521e-50 | -8.615850e-47 |
| Msa0132750 | Msa1442700 | 0.827892 | 1.217321e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATTACAGAACTCTTTA+TGG | 0.136090 | 1_3:-64033556 | Msa0132750:intron |
| CATAACAATGGTTTGAATTC+TGG | 0.146314 | 1_3:-64034186 | Msa0132750:CDS |
| TTTGCAGACCCATCTCTTTC+TGG | 0.168103 | 1_3:-64033530 | Msa0132750:CDS |
| CTTGACATTGTAGGAAATTA+TGG | 0.203840 | 1_3:-64034212 | Msa0132750:intron |
| ATAACAATGGTTTGAATTCT+GGG | 0.303681 | 1_3:-64034185 | Msa0132750:CDS |
| TTCATTACAGAACTCTTTAT+GGG | 0.316740 | 1_3:-64033555 | Msa0132750:intron |
| TCTGGGTTAGATCATACTAA+TGG | 0.367578 | 1_3:-64034168 | Msa0132750:CDS |
| CTGGGTTAGATCATACTAAT+GGG | 0.448053 | 1_3:-64034167 | Msa0132750:CDS |
| TTTATATTCAATAGATGTGG+TGG | 0.536630 | 1_3:-64033832 | Msa0132750:intron |
| TCATTACAGAACTCTTTATG+GGG | 0.577132 | 1_3:-64033554 | Msa0132750:intron |
| CATTACAGAACTCTTTATGG+GGG | 0.611676 | 1_3:-64033553 | Msa0132750:intron |
| AATACATTAATTCAAGCACA+AGG | 0.660463 | 1_3:-64033476 | Msa0132750:CDS |
| AAATTATGGAAGCATAACAA+TGG | 0.673525 | 1_3:-64034198 | Msa0132750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTGATAATTTGATTATA+TGG | + | chr1_3:64034054-64034073 | None:intergenic | 10.0% |
| !!! | ATATTGATAATTTGATTATA+TGG | + | chr1_3:64034054-64034073 | None:intergenic | 10.0% |
| !! | TAATCAAATTATCAATATCA+TGG | - | chr1_3:64034056-64034075 | Msa0132750:intron | 15.0% |
| !! | TAATCAAATTATCAATATCA+TGG | - | chr1_3:64034056-64034075 | Msa0132750:intron | 15.0% |
| !! | GAAAACTTCTTAAAAAAACA+TGG | + | chr1_3:64033510-64033529 | None:intergenic | 20.0% |
| !! | TATCTAGTTATACTATAATG+AGG | - | chr1_3:64034015-64034034 | Msa0132750:intron | 20.0% |
| !! | ATCTAGTTATACTATAATGA+GGG | - | chr1_3:64034016-64034035 | Msa0132750:intron | 20.0% |
| !!! | CTTTTTATATTCAATAGATG+TGG | - | chr1_3:64034092-64034111 | Msa0132750:intron | 20.0% |
| !! | ATGTGTCATAAAGTAAATAA+TGG | + | chr1_3:64034250-64034269 | None:intergenic | 20.0% |
| !!! | CATACATGATTTTTTTTCTT+TGG | + | chr1_3:64034335-64034354 | None:intergenic | 20.0% |
| !!! | AAATCATGTATGAATTTTAC+AGG | - | chr1_3:64034343-64034362 | Msa0132750:intron | 20.0% |
| !! | GAAAACTTCTTAAAAAAACA+TGG | + | chr1_3:64033510-64033529 | None:intergenic | 20.0% |
| !! | TATCTAGTTATACTATAATG+AGG | - | chr1_3:64034015-64034034 | Msa0132750:intron | 20.0% |
| !! | ATCTAGTTATACTATAATGA+GGG | - | chr1_3:64034016-64034035 | Msa0132750:intron | 20.0% |
| !!! | CTTTTTATATTCAATAGATG+TGG | - | chr1_3:64034092-64034111 | Msa0132750:intron | 20.0% |
| !! | ATGTGTCATAAAGTAAATAA+TGG | + | chr1_3:64034250-64034269 | None:intergenic | 20.0% |
| !!! | CATACATGATTTTTTTTCTT+TGG | + | chr1_3:64034335-64034354 | None:intergenic | 20.0% |
| !!! | AAATCATGTATGAATTTTAC+AGG | - | chr1_3:64034343-64034362 | Msa0132750:intron | 20.0% |
| ! | ATGACATAATCAATTTAGCA+TGG | + | chr1_3:64033540-64033559 | None:intergenic | 25.0% |
| ! | TAATGCTTAAGCTTCTAATT+TGG | - | chr1_3:64033609-64033628 | Msa0132750:intron | 25.0% |
| !! | AAATTATGGAAGCATAACAA+TGG | - | chr1_3:64033729-64033748 | Msa0132750:intron | 25.0% |
| ! | ATAACAATGGTTTGAATTCT+GGG | - | chr1_3:64033742-64033761 | Msa0132750:intron | 25.0% |
| ! | TGTCATACTAATTATTACTG+TGG | - | chr1_3:64033839-64033858 | Msa0132750:intron | 25.0% |
| ! | AGAAAGATATCTCCAAATAA+TGG | + | chr1_3:64033958-64033977 | None:intergenic | 25.0% |
| ! | TTTATATTCAATAGATGTGG+TGG | - | chr1_3:64034095-64034114 | Msa0132750:intron | 25.0% |
| ! | TAAATAATGGTGAAAACACA+TGG | + | chr1_3:64034237-64034256 | None:intergenic | 25.0% |
| ! | AATTTCTTTCTTCTAGTACA+CGG | + | chr1_3:64034298-64034317 | None:intergenic | 25.0% |
| ! | TTTCATTACAGAACTCTTTA+TGG | - | chr1_3:64034371-64034390 | Msa0132750:intron | 25.0% |
| ! | TTCATTACAGAACTCTTTAT+GGG | - | chr1_3:64034372-64034391 | Msa0132750:intron | 25.0% |
| ! | AATACATTAATTCAAGCACA+AGG | - | chr1_3:64034451-64034470 | Msa0132750:intron | 25.0% |
| ! | ATGACATAATCAATTTAGCA+TGG | + | chr1_3:64033540-64033559 | None:intergenic | 25.0% |
| ! | TAATGCTTAAGCTTCTAATT+TGG | - | chr1_3:64033609-64033628 | Msa0132750:intron | 25.0% |
| !! | AAATTATGGAAGCATAACAA+TGG | - | chr1_3:64033729-64033748 | Msa0132750:intron | 25.0% |
| ! | ATAACAATGGTTTGAATTCT+GGG | - | chr1_3:64033742-64033761 | Msa0132750:intron | 25.0% |
| ! | TGTCATACTAATTATTACTG+TGG | - | chr1_3:64033839-64033858 | Msa0132750:intron | 25.0% |
| ! | AGAAAGATATCTCCAAATAA+TGG | + | chr1_3:64033958-64033977 | None:intergenic | 25.0% |
| ! | TTTATATTCAATAGATGTGG+TGG | - | chr1_3:64034095-64034114 | Msa0132750:intron | 25.0% |
| ! | TAAATAATGGTGAAAACACA+TGG | + | chr1_3:64034237-64034256 | None:intergenic | 25.0% |
| ! | AATTTCTTTCTTCTAGTACA+CGG | + | chr1_3:64034298-64034317 | None:intergenic | 25.0% |
| ! | TTTCATTACAGAACTCTTTA+TGG | - | chr1_3:64034371-64034390 | Msa0132750:intron | 25.0% |
| ! | TTCATTACAGAACTCTTTAT+GGG | - | chr1_3:64034372-64034391 | Msa0132750:intron | 25.0% |
| ! | AATACATTAATTCAAGCACA+AGG | - | chr1_3:64034451-64034470 | Msa0132750:intron | 25.0% |
| TACCTACCTTGATTTAAACT+TGG | + | chr1_3:64033434-64033453 | None:intergenic | 30.0% | |
| !! | TGTCATACATCATGCTTTTT+AGG | - | chr1_3:64033554-64033573 | Msa0132750:intron | 30.0% |
| GTAGTAACAACAATACTGTT+AGG | + | chr1_3:64033696-64033715 | None:intergenic | 30.0% | |
| TTGTTACTACTTGACATTGT+AGG | - | chr1_3:64033706-64033725 | Msa0132750:intron | 30.0% | |
| CTTGACATTGTAGGAAATTA+TGG | - | chr1_3:64033715-64033734 | Msa0132750:intron | 30.0% | |
| CATAACAATGGTTTGAATTC+TGG | - | chr1_3:64033741-64033760 | Msa0132750:intron | 30.0% | |
| TCAGCAATTTCAATGTCAAA+AGG | + | chr1_3:64033870-64033889 | None:intergenic | 30.0% | |
| ! | TTTTGACATTGAAATTGCTG+AGG | - | chr1_3:64033869-64033888 | Msa0132750:intron | 30.0% |
| ATTTGAATGGGACCATTATT+TGG | - | chr1_3:64033943-64033962 | Msa0132750:intron | 30.0% | |
| !! | ATATCTTTCTAGCTGGAAAT+AGG | - | chr1_3:64033968-64033987 | Msa0132750:intron | 30.0% |
| GCTAACACCAAGAAAATATA+AGG | + | chr1_3:64034197-64034216 | None:intergenic | 30.0% | |
| TCATTACAGAACTCTTTATG+GGG | - | chr1_3:64034373-64034392 | Msa0132750:intron | 30.0% | |
| !! | AACTTTTTCCAGAAAGAGAT+GGG | + | chr1_3:64034408-64034427 | None:intergenic | 30.0% |
| !! | AAACTTTTTCCAGAAAGAGA+TGG | + | chr1_3:64034409-64034428 | None:intergenic | 30.0% |
| !! | TCTTTCTGGAAAAAGTTTTC+TGG | - | chr1_3:64034411-64034430 | Msa0132750:intron | 30.0% |
| TACCTACCTTGATTTAAACT+TGG | + | chr1_3:64033434-64033453 | None:intergenic | 30.0% | |
| !! | TGTCATACATCATGCTTTTT+AGG | - | chr1_3:64033554-64033573 | Msa0132750:intron | 30.0% |
| GTAGTAACAACAATACTGTT+AGG | + | chr1_3:64033696-64033715 | None:intergenic | 30.0% | |
| TTGTTACTACTTGACATTGT+AGG | - | chr1_3:64033706-64033725 | Msa0132750:intron | 30.0% | |
| CTTGACATTGTAGGAAATTA+TGG | - | chr1_3:64033715-64033734 | Msa0132750:intron | 30.0% | |
| CATAACAATGGTTTGAATTC+TGG | - | chr1_3:64033741-64033760 | Msa0132750:intron | 30.0% | |
| TCAGCAATTTCAATGTCAAA+AGG | + | chr1_3:64033870-64033889 | None:intergenic | 30.0% | |
| ! | TTTTGACATTGAAATTGCTG+AGG | - | chr1_3:64033869-64033888 | Msa0132750:intron | 30.0% |
| ATTTGAATGGGACCATTATT+TGG | - | chr1_3:64033943-64033962 | Msa0132750:intron | 30.0% | |
| !! | ATATCTTTCTAGCTGGAAAT+AGG | - | chr1_3:64033968-64033987 | Msa0132750:intron | 30.0% |
| GCTAACACCAAGAAAATATA+AGG | + | chr1_3:64034197-64034216 | None:intergenic | 30.0% | |
| TCATTACAGAACTCTTTATG+GGG | - | chr1_3:64034373-64034392 | Msa0132750:intron | 30.0% | |
| !! | AACTTTTTCCAGAAAGAGAT+GGG | + | chr1_3:64034408-64034427 | None:intergenic | 30.0% |
| !! | AAACTTTTTCCAGAAAGAGA+TGG | + | chr1_3:64034409-64034428 | None:intergenic | 30.0% |
| !! | TCTTTCTGGAAAAAGTTTTC+TGG | - | chr1_3:64034411-64034430 | Msa0132750:intron | 30.0% |
| GTCCAAGTTTAAATCAAGGT+AGG | - | chr1_3:64033429-64033448 | Msa0132750:CDS | 35.0% | |
| ! | TCTGGGTTAGATCATACTAA+TGG | - | chr1_3:64033759-64033778 | Msa0132750:intron | 35.0% |
| ! | CTGGGTTAGATCATACTAAT+GGG | - | chr1_3:64033760-64033779 | Msa0132750:intron | 35.0% |
| !! | CTCTTTTGGTGCATTTGAAT+GGG | - | chr1_3:64033931-64033950 | Msa0132750:intron | 35.0% |
| ! | TTTGGAGATATCTTTCTAGC+TGG | - | chr1_3:64033961-64033980 | Msa0132750:intron | 35.0% |
| !! | TTTCTAGCTGGAAATAGGTT+GGG | - | chr1_3:64033973-64033992 | Msa0132750:intron | 35.0% |
| ! | TCGTGTGCCTTATATTTTCT+TGG | - | chr1_3:64034187-64034206 | Msa0132750:CDS | 35.0% |
| CATTACAGAACTCTTTATGG+GGG | - | chr1_3:64034374-64034393 | Msa0132750:intron | 35.0% | |
| GTCCAAGTTTAAATCAAGGT+AGG | - | chr1_3:64033429-64033448 | Msa0132750:CDS | 35.0% | |
| ! | TCTGGGTTAGATCATACTAA+TGG | - | chr1_3:64033759-64033778 | Msa0132750:intron | 35.0% |
| ! | CTGGGTTAGATCATACTAAT+GGG | - | chr1_3:64033760-64033779 | Msa0132750:intron | 35.0% |
| !! | CTCTTTTGGTGCATTTGAAT+GGG | - | chr1_3:64033931-64033950 | Msa0132750:intron | 35.0% |
| ! | TTTGGAGATATCTTTCTAGC+TGG | - | chr1_3:64033961-64033980 | Msa0132750:intron | 35.0% |
| !! | TTTCTAGCTGGAAATAGGTT+GGG | - | chr1_3:64033973-64033992 | Msa0132750:intron | 35.0% |
| ! | TCGTGTGCCTTATATTTTCT+TGG | - | chr1_3:64034187-64034206 | Msa0132750:CDS | 35.0% |
| CATTACAGAACTCTTTATGG+GGG | - | chr1_3:64034374-64034393 | Msa0132750:intron | 35.0% | |
| AAATTGCTGAGGACAAGCAT+TGG | - | chr1_3:64033880-64033899 | Msa0132750:intron | 40.0% | |
| AATTGCTGAGGACAAGCATT+GGG | - | chr1_3:64033881-64033900 | Msa0132750:intron | 40.0% | |
| TGGGTAGTAAAACGACTCTT+TGG | - | chr1_3:64033900-64033919 | Msa0132750:intron | 40.0% | |
| CCATTCAAATGCACCAAAAG+AGG | + | chr1_3:64033933-64033952 | None:intergenic | 40.0% | |
| !! | CCTCTTTTGGTGCATTTGAA+TGG | - | chr1_3:64033930-64033949 | Msa0132750:intron | 40.0% |
| !! | CTTTCTAGCTGGAAATAGGT+TGG | - | chr1_3:64033972-64033991 | Msa0132750:intron | 40.0% |
| ! | ATTCAATAGATGTGGTGGTG+TGG | - | chr1_3:64034100-64034119 | Msa0132750:intron | 40.0% |
| ! | TGGGAGAGTACAAAGATTAC+AGG | + | chr1_3:64034154-64034173 | None:intergenic | 40.0% |
| AAATTGCTGAGGACAAGCAT+TGG | - | chr1_3:64033880-64033899 | Msa0132750:intron | 40.0% | |
| AATTGCTGAGGACAAGCATT+GGG | - | chr1_3:64033881-64033900 | Msa0132750:intron | 40.0% | |
| TGGGTAGTAAAACGACTCTT+TGG | - | chr1_3:64033900-64033919 | Msa0132750:intron | 40.0% | |
| CCATTCAAATGCACCAAAAG+AGG | + | chr1_3:64033933-64033952 | None:intergenic | 40.0% | |
| !! | CCTCTTTTGGTGCATTTGAA+TGG | - | chr1_3:64033930-64033949 | Msa0132750:intron | 40.0% |
| !! | CTTTCTAGCTGGAAATAGGT+TGG | - | chr1_3:64033972-64033991 | Msa0132750:intron | 40.0% |
| ! | ATTCAATAGATGTGGTGGTG+TGG | - | chr1_3:64034100-64034119 | Msa0132750:intron | 40.0% |
| ! | TGGGAGAGTACAAAGATTAC+AGG | + | chr1_3:64034154-64034173 | None:intergenic | 40.0% |
| ! | CTTTGGTCACAGTCCTCTTT+TGG | - | chr1_3:64033917-64033936 | Msa0132750:intron | 45.0% |
| TTTGTACTCTCCCACTCTTG+TGG | - | chr1_3:64034160-64034179 | Msa0132750:CDS | 45.0% | |
| TTGTACTCTCCCACTCTTGT+GGG | - | chr1_3:64034161-64034180 | Msa0132750:CDS | 45.0% | |
| TTTGCAGACCCATCTCTTTC+TGG | - | chr1_3:64034397-64034416 | Msa0132750:intron | 45.0% | |
| ! | CTTTGGTCACAGTCCTCTTT+TGG | - | chr1_3:64033917-64033936 | Msa0132750:intron | 45.0% |
| TTTGTACTCTCCCACTCTTG+TGG | - | chr1_3:64034160-64034179 | Msa0132750:CDS | 45.0% | |
| TTGTACTCTCCCACTCTTGT+GGG | - | chr1_3:64034161-64034180 | Msa0132750:CDS | 45.0% | |
| TTTGCAGACCCATCTCTTTC+TGG | - | chr1_3:64034397-64034416 | Msa0132750:intron | 45.0% | |
| ! | TGGTGGTGTGGACATCATTC+TGG | - | chr1_3:64034112-64034131 | Msa0132750:intron | 50.0% |
| GGTGGTGTGGACATCATTCT+GGG | - | chr1_3:64034113-64034132 | Msa0132750:intron | 50.0% | |
| ACACGAAGTCCCACAAGAGT+GGG | + | chr1_3:64034173-64034192 | None:intergenic | 50.0% | |
| ! | TGGTGGTGTGGACATCATTC+TGG | - | chr1_3:64034112-64034131 | Msa0132750:intron | 50.0% |
| GGTGGTGTGGACATCATTCT+GGG | - | chr1_3:64034113-64034132 | Msa0132750:intron | 50.0% | |
| ACACGAAGTCCCACAAGAGT+GGG | + | chr1_3:64034173-64034192 | None:intergenic | 50.0% | |
| ! | GCTGGAAATAGGTTGGGTGC+AGG | - | chr1_3:64033979-64033998 | Msa0132750:intron | 55.0% |
| ! | GGAAATAGGTTGGGTGCAGG+TGG | - | chr1_3:64033982-64034001 | Msa0132750:intron | 55.0% |
| CACACGAAGTCCCACAAGAG+TGG | + | chr1_3:64034174-64034193 | None:intergenic | 55.0% | |
| ! | GCTGGAAATAGGTTGGGTGC+AGG | - | chr1_3:64033979-64033998 | Msa0132750:intron | 55.0% |
| ! | GGAAATAGGTTGGGTGCAGG+TGG | - | chr1_3:64033982-64034001 | Msa0132750:intron | 55.0% |
| CACACGAAGTCCCACAAGAG+TGG | + | chr1_3:64034174-64034193 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 64033427 | 64034522 | 64033427 | ID=Msa0132750;Name=Msa0132750 |
| chr1_3 | mRNA | 64033427 | 64034522 | 64033427 | ID=Msa0132750-mRNA-1;Parent=Msa0132750;Name=Msa0132750-mRNA-1;_AED=0.81;_eAED=0.83;_QI=0|0|0|0.75|0|0.75|4|0|72 |
| chr1_3 | exon | 64034515 | 64034522 | 64034515 | ID=Msa0132750-mRNA-1:exon:21464;Parent=Msa0132750-mRNA-1 |
| chr1_3 | exon | 64034160 | 64034221 | 64034160 | ID=Msa0132750-mRNA-1:exon:21463;Parent=Msa0132750-mRNA-1 |
| chr1_3 | exon | 64033833 | 64033840 | 64033833 | ID=Msa0132750-mRNA-1:exon:21462;Parent=Msa0132750-mRNA-1 |
| chr1_3 | exon | 64033427 | 64033567 | 64033427 | ID=Msa0132750-mRNA-1:exon:21461;Parent=Msa0132750-mRNA-1 |
| chr1_3 | CDS | 64034515 | 64034522 | 64034515 | ID=Msa0132750-mRNA-1:cds;Parent=Msa0132750-mRNA-1 |
| chr1_3 | CDS | 64034160 | 64034221 | 64034160 | ID=Msa0132750-mRNA-1:cds;Parent=Msa0132750-mRNA-1 |
| chr1_3 | CDS | 64033833 | 64033840 | 64033833 | ID=Msa0132750-mRNA-1:cds;Parent=Msa0132750-mRNA-1 |
| chr1_3 | CDS | 64033427 | 64033567 | 64033427 | ID=Msa0132750-mRNA-1:cds;Parent=Msa0132750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
