AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1442700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAACAATGGTTTGAATTC+TGG 0.146314 tig0030153:+8716 Msa1442700:CDS
TTTCATTACAGAACTCTTTC+TGG 0.155181 tig0030153:+9348 Msa1442700:intron
TTTGCAGACCCATCTCTTTC+TGG 0.168103 tig0030153:+9374 Msa1442700:CDS
CTTGACATTGTAGGAAATTA+TGG 0.203840 tig0030153:+8690 Msa1442700:intron
ATAACAATGGTTTGAATTCT+GGG 0.303681 tig0030153:+8717 Msa1442700:CDS
TTCATTACAGAACTCTTTCT+GGG 0.360973 tig0030153:+9349 Msa1442700:intron
TCTGGGTTAGATCATACTAA+TGG 0.367578 tig0030153:+8734 Msa1442700:CDS
CTGGGTTAGATCATACTAAT+GGG 0.448053 tig0030153:+8735 Msa1442700:CDS
TTTATATTCAATAGATGTGG+TGG 0.536630 tig0030153:+9070 Msa1442700:intron
CATTACAGAACTCTTTCTGG+GGG 0.560500 tig0030153:+9351 Msa1442700:intron
TCATTACAGAACTCTTTCTG+GGG 0.565777 tig0030153:+9350 Msa1442700:intron
AATACATTAATTCAAGCACA+AGG 0.660463 tig0030153:+9428 Msa1442700:CDS
AAATTATGGAAGCATAACAA+TGG 0.673525 tig0030153:+8704 Msa1442700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGATAATTTGATTATA+TGG - tig0030153:9029-9048 None:intergenic 10.0%
!!! ATATTGATAATTTGATTATA+TGG - tig0030153:9029-9048 None:intergenic 10.0%
!! TAATCAAATTATCAATATCA+TGG + tig0030153:9031-9050 Msa1442700:intron 15.0%
!! TAATCAAATTATCAATATCA+TGG + tig0030153:9031-9050 Msa1442700:intron 15.0%
!! GAAAACTTCTTAAAAAAACA+TGG - tig0030153:8485-8504 None:intergenic 20.0%
!! TATCTAGTTATACTATAATG+AGG + tig0030153:8990-9009 Msa1442700:intron 20.0%
!! ATCTAGTTATACTATAATGA+GGG + tig0030153:8991-9010 Msa1442700:intron 20.0%
!!! CTTTTTATATTCAATAGATG+TGG + tig0030153:9067-9086 Msa1442700:intron 20.0%
!! ATGTGTCATAAAGTAAATAA+TGG - tig0030153:9227-9246 None:intergenic 20.0%
!!! CATACATGATTTTTTTTCTT+TGG - tig0030153:9312-9331 None:intergenic 20.0%
!!! AAATCATGTATGAATTTTAC+AGG + tig0030153:9320-9339 Msa1442700:intron 20.0%
!! GAAAACTTCTTAAAAAAACA+TGG - tig0030153:8485-8504 None:intergenic 20.0%
!! TATCTAGTTATACTATAATG+AGG + tig0030153:8990-9009 Msa1442700:intron 20.0%
!! ATCTAGTTATACTATAATGA+GGG + tig0030153:8991-9010 Msa1442700:intron 20.0%
!!! CTTTTTATATTCAATAGATG+TGG + tig0030153:9067-9086 Msa1442700:intron 20.0%
!! ATGTGTCATAAAGTAAATAA+TGG - tig0030153:9227-9246 None:intergenic 20.0%
!!! CATACATGATTTTTTTTCTT+TGG - tig0030153:9312-9331 None:intergenic 20.0%
!!! AAATCATGTATGAATTTTAC+AGG + tig0030153:9320-9339 Msa1442700:intron 20.0%
! ATGACATAATCAATTTAGCA+TGG - tig0030153:8515-8534 None:intergenic 25.0%
! TAATGCTTAAGCTTCTAATT+TGG + tig0030153:8584-8603 Msa1442700:intron 25.0%
!! AAATTATGGAAGCATAACAA+TGG + tig0030153:8704-8723 Msa1442700:CDS 25.0%
! ATAACAATGGTTTGAATTCT+GGG + tig0030153:8717-8736 Msa1442700:CDS 25.0%
! TGTCATACTAATTATTACTG+TGG + tig0030153:8814-8833 Msa1442700:intron 25.0%
! AGAAAGATATCTCCAAATAA+TGG - tig0030153:8933-8952 None:intergenic 25.0%
! TTTATATTCAATAGATGTGG+TGG + tig0030153:9070-9089 Msa1442700:intron 25.0%
! AATAATGGTGAAAACACATA+TGG - tig0030153:9212-9231 None:intergenic 25.0%
! AATTTCTTTCTTCTAGTACA+CGG - tig0030153:9275-9294 None:intergenic 25.0%
! AATACATTAATTCAAGCACA+AGG + tig0030153:9428-9447 Msa1442700:CDS 25.0%
! ATGACATAATCAATTTAGCA+TGG - tig0030153:8515-8534 None:intergenic 25.0%
! TAATGCTTAAGCTTCTAATT+TGG + tig0030153:8584-8603 Msa1442700:intron 25.0%
!! AAATTATGGAAGCATAACAA+TGG + tig0030153:8704-8723 Msa1442700:CDS 25.0%
! ATAACAATGGTTTGAATTCT+GGG + tig0030153:8717-8736 Msa1442700:CDS 25.0%
! TGTCATACTAATTATTACTG+TGG + tig0030153:8814-8833 Msa1442700:intron 25.0%
! AGAAAGATATCTCCAAATAA+TGG - tig0030153:8933-8952 None:intergenic 25.0%
! TTTATATTCAATAGATGTGG+TGG + tig0030153:9070-9089 Msa1442700:intron 25.0%
! AATAATGGTGAAAACACATA+TGG - tig0030153:9212-9231 None:intergenic 25.0%
! AATTTCTTTCTTCTAGTACA+CGG - tig0030153:9275-9294 None:intergenic 25.0%
! AATACATTAATTCAAGCACA+AGG + tig0030153:9428-9447 Msa1442700:CDS 25.0%
TACCTACCTTGATTTAAACT+TGG - tig0030153:8409-8428 None:intergenic 30.0%
!! TGTCATACATCATGCTTTTT+AGG + tig0030153:8529-8548 Msa1442700:intron 30.0%
TTTAGGTGCGATATTATCAA+TGG + tig0030153:8546-8565 Msa1442700:intron 30.0%
GTAGTAACAACAATACTGTT+AGG - tig0030153:8671-8690 None:intergenic 30.0%
TTGTTACTACTTGACATTGT+AGG + tig0030153:8681-8700 Msa1442700:intron 30.0%
CTTGACATTGTAGGAAATTA+TGG + tig0030153:8690-8709 Msa1442700:intron 30.0%
CATAACAATGGTTTGAATTC+TGG + tig0030153:8716-8735 Msa1442700:CDS 30.0%
TCAGCAATTTCAATGTCAAA+AGG - tig0030153:8845-8864 None:intergenic 30.0%
! TTTTGACATTGAAATTGCTG+AGG + tig0030153:8844-8863 Msa1442700:intron 30.0%
ATTTGAATGGGACCATTATT+TGG + tig0030153:8918-8937 Msa1442700:intron 30.0%
!! ATATCTTTCTAGCTGGAAAT+AGG + tig0030153:8943-8962 Msa1442700:intron 30.0%
GCTAACACCAAGAAAATATA+AGG - tig0030153:9172-9191 None:intergenic 30.0%
TTTCATTACAGAACTCTTTC+TGG + tig0030153:9348-9367 Msa1442700:intron 30.0%
TTCATTACAGAACTCTTTCT+GGG + tig0030153:9349-9368 Msa1442700:intron 30.0%
!! AACTTTTTCCAGAAAGAGAT+GGG - tig0030153:9385-9404 None:intergenic 30.0%
!! AAACTTTTTCCAGAAAGAGA+TGG - tig0030153:9386-9405 None:intergenic 30.0%
!! TCTTTCTGGAAAAAGTTTTC+TGG + tig0030153:9388-9407 Msa1442700:CDS 30.0%
TACCTACCTTGATTTAAACT+TGG - tig0030153:8409-8428 None:intergenic 30.0%
!! TGTCATACATCATGCTTTTT+AGG + tig0030153:8529-8548 Msa1442700:intron 30.0%
TTTAGGTGCGATATTATCAA+TGG + tig0030153:8546-8565 Msa1442700:intron 30.0%
GTAGTAACAACAATACTGTT+AGG - tig0030153:8671-8690 None:intergenic 30.0%
TTGTTACTACTTGACATTGT+AGG + tig0030153:8681-8700 Msa1442700:intron 30.0%
CTTGACATTGTAGGAAATTA+TGG + tig0030153:8690-8709 Msa1442700:intron 30.0%
CATAACAATGGTTTGAATTC+TGG + tig0030153:8716-8735 Msa1442700:CDS 30.0%
TCAGCAATTTCAATGTCAAA+AGG - tig0030153:8845-8864 None:intergenic 30.0%
! TTTTGACATTGAAATTGCTG+AGG + tig0030153:8844-8863 Msa1442700:intron 30.0%
ATTTGAATGGGACCATTATT+TGG + tig0030153:8918-8937 Msa1442700:intron 30.0%
!! ATATCTTTCTAGCTGGAAAT+AGG + tig0030153:8943-8962 Msa1442700:intron 30.0%
GCTAACACCAAGAAAATATA+AGG - tig0030153:9172-9191 None:intergenic 30.0%
TTTCATTACAGAACTCTTTC+TGG + tig0030153:9348-9367 Msa1442700:intron 30.0%
TTCATTACAGAACTCTTTCT+GGG + tig0030153:9349-9368 Msa1442700:intron 30.0%
!! AACTTTTTCCAGAAAGAGAT+GGG - tig0030153:9385-9404 None:intergenic 30.0%
!! AAACTTTTTCCAGAAAGAGA+TGG - tig0030153:9386-9405 None:intergenic 30.0%
!! TCTTTCTGGAAAAAGTTTTC+TGG + tig0030153:9388-9407 Msa1442700:CDS 30.0%
GTCCAAGTTTAAATCAAGGT+AGG + tig0030153:8404-8423 Msa1442700:intron 35.0%
AGATAAAAAGCTTGAGCGTT+TGG - tig0030153:8435-8454 None:intergenic 35.0%
! TCTGGGTTAGATCATACTAA+TGG + tig0030153:8734-8753 Msa1442700:CDS 35.0%
! CTGGGTTAGATCATACTAAT+GGG + tig0030153:8735-8754 Msa1442700:CDS 35.0%
!! CTCTTTTGGTGCATTTGAAT+GGG + tig0030153:8906-8925 Msa1442700:intron 35.0%
! TTTGGAGATATCTTTCTAGC+TGG + tig0030153:8936-8955 Msa1442700:intron 35.0%
!! TTTCTAGCTGGAAATAGGTT+GGG + tig0030153:8948-8967 Msa1442700:intron 35.0%
! TCGTGTGCCTTATATTTTCT+TGG + tig0030153:9162-9181 Msa1442700:intron 35.0%
TCATTACAGAACTCTTTCTG+GGG + tig0030153:9350-9369 Msa1442700:intron 35.0%
GTCCAAGTTTAAATCAAGGT+AGG + tig0030153:8404-8423 Msa1442700:intron 35.0%
AGATAAAAAGCTTGAGCGTT+TGG - tig0030153:8435-8454 None:intergenic 35.0%
! TCTGGGTTAGATCATACTAA+TGG + tig0030153:8734-8753 Msa1442700:CDS 35.0%
! CTGGGTTAGATCATACTAAT+GGG + tig0030153:8735-8754 Msa1442700:CDS 35.0%
!! CTCTTTTGGTGCATTTGAAT+GGG + tig0030153:8906-8925 Msa1442700:intron 35.0%
! TTTGGAGATATCTTTCTAGC+TGG + tig0030153:8936-8955 Msa1442700:intron 35.0%
!! TTTCTAGCTGGAAATAGGTT+GGG + tig0030153:8948-8967 Msa1442700:intron 35.0%
! TCGTGTGCCTTATATTTTCT+TGG + tig0030153:9162-9181 Msa1442700:intron 35.0%
TCATTACAGAACTCTTTCTG+GGG + tig0030153:9350-9369 Msa1442700:intron 35.0%
AAATTGCTGAGGACAAGCAT+TGG + tig0030153:8855-8874 Msa1442700:intron 40.0%
AATTGCTGAGGACAAGCATT+GGG + tig0030153:8856-8875 Msa1442700:intron 40.0%
TGGGTAGTAAAACGACTCTT+TGG + tig0030153:8875-8894 Msa1442700:intron 40.0%
CCATTCAAATGCACCAAAAG+AGG - tig0030153:8908-8927 None:intergenic 40.0%
!! CCTCTTTTGGTGCATTTGAA+TGG + tig0030153:8905-8924 Msa1442700:intron 40.0%
!! CTTTCTAGCTGGAAATAGGT+TGG + tig0030153:8947-8966 Msa1442700:intron 40.0%
! ATTCAATAGATGTGGTGGTG+TGG + tig0030153:9075-9094 Msa1442700:intron 40.0%
! TGGGAGAGTACAAAGATTAC+AGG - tig0030153:9129-9148 None:intergenic 40.0%
CATTACAGAACTCTTTCTGG+GGG + tig0030153:9351-9370 Msa1442700:intron 40.0%
AAATTGCTGAGGACAAGCAT+TGG + tig0030153:8855-8874 Msa1442700:intron 40.0%
AATTGCTGAGGACAAGCATT+GGG + tig0030153:8856-8875 Msa1442700:intron 40.0%
TGGGTAGTAAAACGACTCTT+TGG + tig0030153:8875-8894 Msa1442700:intron 40.0%
CCATTCAAATGCACCAAAAG+AGG - tig0030153:8908-8927 None:intergenic 40.0%
!! CCTCTTTTGGTGCATTTGAA+TGG + tig0030153:8905-8924 Msa1442700:intron 40.0%
!! CTTTCTAGCTGGAAATAGGT+TGG + tig0030153:8947-8966 Msa1442700:intron 40.0%
! ATTCAATAGATGTGGTGGTG+TGG + tig0030153:9075-9094 Msa1442700:intron 40.0%
! TGGGAGAGTACAAAGATTAC+AGG - tig0030153:9129-9148 None:intergenic 40.0%
CATTACAGAACTCTTTCTGG+GGG + tig0030153:9351-9370 Msa1442700:intron 40.0%
! CTTTGGTCACAGTCCTCTTT+TGG + tig0030153:8892-8911 Msa1442700:intron 45.0%
TTTGTACTCTCCCACTCTTG+TGG + tig0030153:9135-9154 Msa1442700:intron 45.0%
TTGTACTCTCCCACTCTTGT+GGG + tig0030153:9136-9155 Msa1442700:intron 45.0%
TTTGCAGACCCATCTCTTTC+TGG + tig0030153:9374-9393 Msa1442700:CDS 45.0%
! CTTTGGTCACAGTCCTCTTT+TGG + tig0030153:8892-8911 Msa1442700:intron 45.0%
TTTGTACTCTCCCACTCTTG+TGG + tig0030153:9135-9154 Msa1442700:intron 45.0%
TTGTACTCTCCCACTCTTGT+GGG + tig0030153:9136-9155 Msa1442700:intron 45.0%
TTTGCAGACCCATCTCTTTC+TGG + tig0030153:9374-9393 Msa1442700:CDS 45.0%
ACACGAAGTCCCACAAGAGT+GGG - tig0030153:9148-9167 None:intergenic 50.0%
ACACGAAGTCCCACAAGAGT+GGG - tig0030153:9148-9167 None:intergenic 50.0%
! GCTGGAAATAGGTTGGGTGC+AGG + tig0030153:8954-8973 Msa1442700:intron 55.0%
! GGAAATAGGTTGGGTGCAGG+TGG + tig0030153:8957-8976 Msa1442700:intron 55.0%
CACACGAAGTCCCACAAGAG+TGG - tig0030153:9149-9168 None:intergenic 55.0%
! GCTGGAAATAGGTTGGGTGC+AGG + tig0030153:8954-8973 Msa1442700:intron 55.0%
! GGAAATAGGTTGGGTGCAGG+TGG + tig0030153:8957-8976 Msa1442700:intron 55.0%
CACACGAAGTCCCACAAGAG+TGG - tig0030153:9149-9168 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0030153 gene 8402 9499 8402 ID=Msa1442700;Name=Msa1442700
tig0030153 mRNA 8402 9499 8402 ID=Msa1442700-mRNA-1;Parent=Msa1442700;Name=Msa1442700-mRNA-1;_AED=0.81;_eAED=0.83;_QI=0|0|0|0.75|0|0.25|4|0|72
tig0030153 exon 8402 8409 8402 ID=Msa1442700-mRNA-1:exon:13478;Parent=Msa1442700-mRNA-1
tig0030153 exon 8703 8764 8703 ID=Msa1442700-mRNA-1:exon:13479;Parent=Msa1442700-mRNA-1
tig0030153 exon 9084 9091 9084 ID=Msa1442700-mRNA-1:exon:13480;Parent=Msa1442700-mRNA-1
tig0030153 exon 9359 9499 9359 ID=Msa1442700-mRNA-1:exon:13481;Parent=Msa1442700-mRNA-1
tig0030153 CDS 8402 8409 8402 ID=Msa1442700-mRNA-1:cds;Parent=Msa1442700-mRNA-1
tig0030153 CDS 8703 8764 8703 ID=Msa1442700-mRNA-1:cds;Parent=Msa1442700-mRNA-1
tig0030153 CDS 9084 9091 9084 ID=Msa1442700-mRNA-1:cds;Parent=Msa1442700-mRNA-1
tig0030153 CDS 9359 9499 9359 ID=Msa1442700-mRNA-1:cds;Parent=Msa1442700-mRNA-1
Gene Sequence

>Msa1442700

ATGTCCAAGAAATTATGGAAGCATAACAATGGTTTGAATTCTGGGTTAGATCATACTAATGGGATCTCAAATGTGGTGAACTCTTTCTGGGGGTTTGCAGACCCATCTCTTTCTGGAAAAAGTTTTCTGGAAAAGTCTAAACGAATTAATACATTAATTCAAGCACAAGGCAGTATGCTGCAACTCTGCAGTGATCACTTTCAATATGCTGTCATGTAA

Protein sequence

>Msa1442700

MSKKLWKHNNGLNSGLDHTNGISNVVNSFWGFADPSLSGKSFLEKSKRINTLIQAQGSMLQLCSDHFQYAVM*