AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136280 MtrunA17_Chr1g0207571 99.208 379 3 0 1 379 1 379 0.0 771
Msa0136280 MtrunA17_Chr1g0213351 58.735 332 127 2 17 340 28 357 2.20e-138 399
Msa0136280 MtrunA17_Chr1g0211251 53.743 374 158 3 2 362 9 380 1.62e-137 397
Msa0136280 MtrunA17_Chr7g0250061 51.459 377 161 3 6 362 1 375 8.29e-135 390
Msa0136280 MtrunA17_Chr8g0371321 55.455 330 137 2 17 338 27 354 4.55e-130 378
Msa0136280 MtrunA17_Chr1g0175381 54.969 322 142 1 18 339 36 354 4.88e-129 375
Msa0136280 MtrunA17_Chr2g0294291 53.538 325 148 2 16 339 30 352 1.33e-123 361
Msa0136280 MtrunA17_Chr7g0263281 54.683 331 140 2 16 338 18 346 2.21e-118 348
Msa0136280 MtrunA17_Chr1g0181901 50.140 357 139 4 20 339 27 381 7.00e-112 332
Msa0136280 MtrunA17_Chr3g0089711 46.875 320 159 5 27 339 33 348 2.84e-94 286
Msa0136280 MtrunA17_Chr3g0089721 46.875 320 159 5 27 339 33 348 4.65e-94 286
Msa0136280 MtrunA17_Chr7g0256751 47.040 321 157 6 27 339 34 349 2.53e-86 268
Msa0136280 MtrunA17_Chr7g0256771 47.744 266 132 4 27 289 35 296 6.60e-75 234
Msa0136280 MtrunA17_Chr3g0089691 43.357 286 152 4 81 360 2 283 2.71e-71 225
Msa0136280 MtrunA17_Chr4g0063111 35.326 368 216 9 20 379 34 387 2.11e-62 208
Msa0136280 MtrunA17_Chr4g0063051 33.437 323 175 10 23 339 22 310 5.39e-47 163
Msa0136280 MtrunA17_Chr7g0256761 44.444 180 79 4 163 337 17 180 8.94e-39 137
Msa0136280 MtrunA17_Chr7g0238501 31.208 298 172 10 61 338 263 547 9.35e-37 140
Msa0136280 MtrunA17_Chr2g0295991 34.241 257 149 6 98 344 237 483 1.34e-36 139
Msa0136280 MtrunA17_Chr4g0067301 31.274 259 156 7 98 344 246 494 1.32e-31 125
Msa0136280 MtrunA17_Chr4g0039061 30.137 292 180 7 61 338 202 483 3.15e-31 124
Msa0136280 MtrunA17_Chr1g0193691 29.085 306 154 5 99 346 592 892 1.17e-30 124
Msa0136280 MtrunA17_Chr4g0031521 31.769 277 147 7 98 338 426 696 4.66e-29 119
Msa0136280 MtrunA17_Chr4g0054811 29.412 306 159 6 95 348 478 778 1.58e-28 117
Msa0136280 MtrunA17_Chr3g0105351 29.930 284 135 9 61 340 94 317 4.18e-26 106
Msa0136280 MtrunA17_Chr3g0105371 29.825 285 136 8 60 340 117 341 2.41e-25 105
Msa0136280 MtrunA17_Chr4g0062951 33.161 193 103 7 95 271 1 183 6.73e-25 100
Msa0136280 MtrunA17_Chr1g0212961 29.412 289 133 9 61 346 126 346 1.18e-24 103
Msa0136280 MtrunA17_Chr5g0430481 28.269 283 141 8 61 340 154 377 1.48e-23 100
Msa0136280 MtrunA17_Chr1g0150451 29.617 287 133 9 63 344 110 332 2.56e-21 93.6
Msa0136280 MtrunA17_Chr3g0112291 26.453 344 190 13 29 345 78 385 1.09e-20 92.8
Msa0136280 MtrunA17_Chr4g0070871 30.453 243 113 8 57 286 249 448 1.38e-20 93.2
Msa0136280 MtrunA17_Chr3g0126511 28.163 245 125 7 46 283 106 306 1.56e-20 92.0
Msa0136280 MtrunA17_Chr3g0135961 28.947 304 153 12 54 337 279 539 3.76e-20 92.0
Msa0136280 MtrunA17_Chr3g0136711 29.252 294 140 10 63 352 67 296 4.54e-20 89.7
Msa0136280 MtrunA17_Chr1g0194191 29.316 307 139 11 54 343 112 357 1.43e-19 89.4
Msa0136280 MtrunA17_Chr2g0293811 28.819 288 151 9 54 337 94 331 3.36e-19 88.2
Msa0136280 MtrunA17_Chr5g0407161 27.986 293 135 13 57 339 121 347 1.51e-17 83.2
Msa0136280 MtrunA17_Chr4g0050381 28.322 286 139 10 36 306 32 266 3.29e-17 80.9
Msa0136280 MtrunA17_Chr4g0001761 27.113 284 131 9 46 284 35 287 3.81e-17 82.0
Msa0136280 MtrunA17_Chr3g0136811 26.316 285 132 9 46 284 35 287 6.34e-17 81.3
Msa0136280 MtrunA17_Chr5g0435721 28.017 232 124 6 61 284 223 419 1.09e-16 81.3
Msa0136280 MtrunA17_Chr2g0317021 27.500 280 130 10 64 337 120 332 1.71e-15 76.6
Msa0136280 MtrunA17_Chr1g0150071 27.341 267 126 7 58 284 49 287 2.60e-15 76.6
Msa0136280 MtrunA17_Chr5g0426141 25.974 308 155 11 36 335 37 279 4.54e-15 74.7
Msa0136280 MtrunA17_Chr1g0171571 27.273 297 144 11 52 337 409 644 5.43e-15 76.6
Msa0136280 MtrunA17_Chr1g0158781 28.390 236 127 5 61 294 145 340 7.23e-15 75.5
Msa0136280 MtrunA17_Chr4g0051661 25.952 289 139 11 31 300 149 381 1.22e-14 74.7
Msa0136280 MtrunA17_Chr2g0279151 25.733 307 157 12 36 335 36 278 1.81e-14 73.2
Msa0136280 MtrunA17_Chr3g0144111 27.273 286 117 12 57 335 24 225 3.28e-14 72.0
Msa0136280 MtrunA17_Chr8g0360351 25.965 285 155 11 28 302 29 267 9.09e-14 71.2
Msa0136280 MtrunA17_Chr3g0127091 27.755 245 137 7 60 284 88 312 2.07e-13 70.9
Msa0136280 MtrunA17_Chr3g0115851 26.433 314 146 13 36 335 37 279 5.88e-13 68.6
Msa0136280 MtrunA17_Chr8g0389921 26.222 225 118 6 61 278 198 381 2.36e-12 68.2
Msa0136280 MtrunA17_Chr4g0022171 24.405 336 191 13 54 360 89 390 2.44e-12 68.2
Msa0136280 MtrunA17_Chr6g0483411 24.138 319 165 11 46 340 56 321 8.08e-12 65.9
Msa0136280 MtrunA17_Chr2g0295401 26.073 303 155 13 62 337 93 353 1.11e-11 65.5
Msa0136280 MtrunA17_Chr7g0221691 33.129 163 85 6 142 301 245 386 1.30e-11 65.9
Msa0136280 MtrunA17_Chr7g0272061 25.949 316 150 16 61 337 91 361 4.88e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136280 AT4G33920.1 70.712 379 109 2 1 378 1 378 0.0 549
Msa0136280 AT4G33920.2 70.169 295 86 2 85 378 2 295 3.01e-147 419
Msa0136280 AT3G51370.1 54.648 355 148 3 1 344 8 360 4.66e-140 404
Msa0136280 AT3G51370.3 54.648 355 148 3 1 344 8 360 4.66e-140 404
Msa0136280 AT5G66080.1 56.196 347 141 2 16 353 28 372 1.10e-139 403
Msa0136280 AT3G12620.2 57.270 337 134 2 17 345 31 365 2.37e-135 392
Msa0136280 AT3G12620.1 57.270 337 134 2 17 345 31 365 2.37e-135 392
Msa0136280 AT4G33920.3 71.053 266 75 2 114 378 1 265 9.38e-134 383
Msa0136280 AT3G55050.1 57.447 329 130 2 17 337 32 358 2.72e-133 387
Msa0136280 AT3G55050.2 57.447 329 130 2 17 337 32 358 2.72e-133 387
Msa0136280 AT4G38520.2 53.955 354 153 2 17 362 29 380 1.31e-131 383
Msa0136280 AT4G38520.1 53.955 354 153 2 17 362 29 380 1.31e-131 383
Msa0136280 AT5G06750.3 57.053 319 136 1 22 339 43 361 3.84e-129 376
Msa0136280 AT5G06750.1 57.053 319 136 1 22 339 43 361 3.84e-129 376
Msa0136280 AT3G17090.1 54.985 331 144 3 22 351 45 371 5.56e-128 373
Msa0136280 AT5G02760.1 53.799 329 140 3 20 340 23 347 3.89e-117 345
Msa0136280 AT3G51370.2 55.797 276 114 3 74 344 3 275 2.65e-109 322
Msa0136280 AT5G66080.2 56.767 266 113 1 88 353 5 268 6.25e-109 321
Msa0136280 AT5G06750.2 57.200 250 106 1 91 339 30 279 3.36e-98 295
Msa0136280 AT3G17090.2 52.963 270 123 2 22 290 45 311 3.75e-98 295
Msa0136280 AT3G16560.1 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0136280 AT3G16560.4 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0136280 AT3G16560.2 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0136280 AT5G02400.1 31.884 276 148 6 98 338 396 666 5.92e-33 130
Msa0136280 AT2G46920.3 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0136280 AT2G46920.1 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0136280 AT2G46920.2 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0136280 AT2G46920.4 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0136280 AT2G35350.1 28.328 293 157 5 93 337 483 770 9.13e-32 127
Msa0136280 AT2G28890.1 31.317 281 150 6 96 338 371 646 3.61e-31 125
Msa0136280 AT3G09400.1 32.090 268 144 7 100 338 384 642 1.25e-30 124
Msa0136280 AT1G07630.1 31.159 276 150 5 98 338 384 654 1.40e-30 123
Msa0136280 AT2G40180.1 30.142 282 135 9 61 337 161 385 4.23e-28 114
Msa0136280 AT1G67820.2 31.461 267 128 7 60 316 114 335 7.03e-28 113
Msa0136280 AT1G67820.1 31.461 267 128 7 60 316 150 371 9.99e-28 114
Msa0136280 AT3G16560.3 33.641 217 128 4 98 306 245 453 5.57e-26 108
Msa0136280 AT1G07160.1 29.000 300 148 9 46 337 134 376 9.23e-26 107
Msa0136280 AT2G30020.1 30.605 281 133 8 63 339 172 394 4.36e-23 100
Msa0136280 AT3G09400.2 29.851 268 145 10 100 337 384 638 7.20e-22 97.8
Msa0136280 AT4G31750.2 31.048 248 121 8 63 305 66 268 1.62e-21 94.4
Msa0136280 AT4G31750.1 31.048 248 121 8 63 305 66 268 1.63e-21 94.4
Msa0136280 AT4G31750.3 31.048 248 121 8 63 305 66 268 1.63e-21 94.4
Msa0136280 AT1G43900.1 29.893 281 129 10 61 338 154 369 9.56e-21 93.2
Msa0136280 AT1G07430.1 28.520 277 145 9 25 284 114 354 3.60e-20 92.0
Msa0136280 AT2G33700.1 28.767 292 139 11 51 335 113 342 4.73e-19 88.2
Msa0136280 AT2G29380.1 27.124 306 156 12 54 337 100 360 3.48e-18 85.5
Msa0136280 AT5G10740.1 27.536 276 133 8 63 335 66 277 9.10e-18 84.3
Msa0136280 AT5G10740.2 27.536 276 133 8 63 335 66 277 9.10e-18 84.3
Msa0136280 AT1G48040.1 29.730 296 152 10 54 343 110 355 2.35e-17 83.2
Msa0136280 AT3G62260.3 30.315 254 137 10 57 304 24 243 3.16e-17 81.6
Msa0136280 AT5G53140.1 26.531 294 148 10 63 353 134 362 5.73e-17 82.4
Msa0136280 AT5G53140.2 26.531 294 148 10 63 353 134 362 5.73e-17 82.4
Msa0136280 AT2G25620.1 26.408 284 154 11 57 337 125 356 6.23e-17 82.0
Msa0136280 AT2G25620.2 26.408 284 154 11 57 337 125 356 6.23e-17 82.0
Msa0136280 AT4G26080.1 29.153 295 153 13 58 337 169 422 8.14e-17 82.0
Msa0136280 AT3G62260.1 30.315 254 137 10 57 304 113 332 1.01e-16 81.3
Msa0136280 AT5G51760.2 28.099 242 121 9 61 283 114 321 1.07e-16 81.3
Msa0136280 AT3G62260.2 30.315 254 137 10 57 304 114 333 1.20e-16 81.3
Msa0136280 AT5G24940.1 27.536 276 133 8 63 335 66 277 1.34e-16 81.3
Msa0136280 AT5G51760.1 28.099 242 121 9 61 283 144 351 1.38e-16 81.3
Msa0136280 AT5G59220.1 28.333 240 125 8 54 284 141 342 1.53e-16 80.9
Msa0136280 AT1G18030.1 26.990 289 146 10 43 303 83 334 2.15e-15 77.0
Msa0136280 AT4G31860.3 25.818 275 120 11 58 284 49 287 3.55e-15 75.9
Msa0136280 AT4G31860.1 25.818 275 120 11 58 284 49 287 6.78e-15 75.9
Msa0136280 AT3G51470.2 25.168 298 150 10 47 335 48 281 7.40e-15 75.1
Msa0136280 AT3G51470.1 25.168 298 150 10 47 335 92 325 1.12e-14 75.1
Msa0136280 AT1G78200.1 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0136280 AT1G78200.3 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0136280 AT1G78200.2 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0136280 AT3G16800.2 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0136280 AT3G16800.1 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0136280 AT3G16800.3 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0136280 AT5G57050.1 25.597 293 167 11 58 337 157 411 2.96e-14 74.3
Msa0136280 AT5G57050.2 25.597 293 167 11 58 337 117 371 3.10e-14 73.9
Msa0136280 AT4G08260.1 26.715 277 116 8 63 339 21 210 4.62e-14 71.2
Msa0136280 AT2G40860.1 28.369 282 137 10 63 337 425 648 9.96e-14 73.2
Msa0136280 AT2G40860.4 28.369 282 137 10 63 337 327 550 9.98e-14 73.2
Msa0136280 AT4G28400.3 27.309 249 129 10 64 306 48 250 1.83e-13 70.5
Msa0136280 AT3G16800.4 28.571 252 124 11 98 337 46 253 1.87e-13 70.1
Msa0136280 AT3G16800.6 28.571 252 124 11 98 337 46 253 1.87e-13 70.1
Msa0136280 AT4G31860.2 26.236 263 110 11 58 272 49 275 2.61e-13 70.1
Msa0136280 AT4G28400.1 27.309 249 129 10 64 306 70 272 2.69e-13 70.1
Msa0136280 AT2G25070.1 26.277 274 118 12 59 284 50 287 3.85e-13 70.5
Msa0136280 AT2G25070.2 26.277 274 118 12 59 284 50 287 3.85e-13 70.5
Msa0136280 AT2G40860.2 27.823 248 136 7 63 303 425 636 4.74e-13 70.9
Msa0136280 AT5G26010.1 26.441 295 174 10 52 337 64 324 5.12e-13 69.7
Msa0136280 AT1G22280.3 26.855 283 153 11 36 306 36 276 9.38e-13 68.6
Msa0136280 AT1G03590.1 26.361 349 186 14 54 371 83 391 2.13e-12 68.6
Msa0136280 AT1G18030.2 27.200 250 130 9 43 273 83 299 2.20e-12 67.8
Msa0136280 AT5G01700.1 26.531 294 169 11 61 339 29 290 2.38e-12 67.8
Msa0136280 AT5G01700.2 26.531 294 169 11 61 339 78 339 2.59e-12 68.2
Msa0136280 AT1G78200.5 26.996 263 138 11 54 306 9 227 3.36e-12 66.2
Msa0136280 AT1G78200.4 26.996 263 138 11 54 306 9 227 3.36e-12 66.2
Msa0136280 AT1G17550.1 25.472 318 158 13 56 337 227 501 8.26e-12 67.0
Msa0136280 AT1G17550.2 25.472 318 158 13 56 337 227 501 8.26e-12 67.0
Msa0136280 AT4G27800.3 27.711 249 136 9 58 284 85 311 1.09e-11 65.9
Msa0136280 AT4G27800.2 27.711 249 136 9 58 284 85 311 1.24e-11 65.9
Msa0136280 AT1G72770.4 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0136280 AT1G72770.1 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0136280 AT1G72770.3 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0136280 AT2G34740.1 23.642 313 156 10 36 335 90 332 1.60e-11 65.5
Msa0136280 AT4G27800.1 27.106 273 155 9 58 308 85 335 1.95e-11 65.5

Find 141 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATAAGCTGACCTTATTT+TGG 0.149456 1_3:+68111102 Msa0136280:three_prime_UTR
GTGGGATCATGTTGTCTTCT+TGG 0.195703 1_3:+68109870 Msa0136280:CDS
AGATACTGACTGTGTAAATA+AGG 0.264924 1_3:-68109262 None:intergenic
TGCCGTGCTATTCAGATGTT+TGG 0.272533 1_3:+68111652 Msa0136280:three_prime_UTR
TTGGTAAGTTAAGAGAATTC+TGG 0.274260 1_3:+68111157 Msa0136280:three_prime_UTR
ACTAGTAAGAGCTGCTCTTC+AGG 0.291775 1_3:+68110825 Msa0136280:CDS
GATGGTTCAGCTGTCATTAC+TGG 0.293354 1_3:-68110577 None:intergenic
CTGAACCATCAATCATCATT+AGG 0.315695 1_3:+68110590 Msa0136280:CDS
GACGCAATCTGAGGACTAAT+TGG 0.330240 1_3:-68109846 None:intergenic
GGTGACTCTAGAGTTGTTCT+TGG 0.332422 1_3:+68109930 Msa0136280:CDS
TATAGATGATGATGGTGATT+AGG 0.354348 1_3:-68108940 None:intergenic
TATTTGCATCAGATGGCCTT+TGG 0.354565 1_3:+68110641 Msa0136280:CDS
TTAATTGATTAGTCCTTGTA+TGG 0.354606 1_3:+68111343 Msa0136280:three_prime_UTR
TTGACAAATCTAGAAGCTTC+AGG 0.361584 1_3:-68109226 None:intergenic
GCTAATGTTCGTGAATGATA+TGG 0.363428 1_3:+68111373 Msa0136280:three_prime_UTR
TTTAGAGAAAATATATCAAC+AGG 0.367089 1_3:-68111015 None:intergenic
TGAAATGAAATGAAAAGAAA+TGG 0.372964 1_3:-68108902 None:intergenic
AAACAAAAGAGAAGAAGGAA+AGG 0.384883 1_3:+68108814 Msa0136280:five_prime_UTR
TCGTTGTCCAACTTGTGTTT+CGG 0.391265 1_3:+68111697 Msa0136280:three_prime_UTR
ATTTGCATCAGATGGCCTTT+GGG 0.407098 1_3:+68110642 Msa0136280:CDS
ACTGGACGCTTCAAAGGAAT+AGG 0.409207 1_3:-68110559 None:intergenic
TGGGTGAATGCCGCGTTTAG+AGG 0.409840 1_3:-68108870 None:intergenic
GGAAAATGATTAATAGTAAA+GGG 0.415664 1_3:+68108774 None:intergenic
ATGGGATCTCTGTAGAAATC+TGG 0.417500 1_3:-68110517 None:intergenic
ACCACAATGTGTGAATCATC+AGG 0.421762 1_3:-68110059 None:intergenic
GCCGTGGTGTTTGTTTGAAA+GGG 0.421904 1_3:-68111751 None:intergenic
AGCTCCCTAATGATGATTGA+TGG 0.423360 1_3:-68110595 None:intergenic
TGGTTTATAGCCGTGGTGTT+TGG 0.426614 1_3:+68110078 Msa0136280:CDS
GGCCGTGGTGTTTGTTTGAA+AGG 0.429085 1_3:-68111752 None:intergenic
TGAACCATCAATCATCATTA+GGG 0.429934 1_3:+68110591 Msa0136280:CDS
GAATGATATGGTACGAGGTA+AGG 0.432229 1_3:+68111385 Msa0136280:three_prime_UTR
CATAAGATACTCACAGCTCT+TGG 0.439024 1_3:-68110709 None:intergenic
TTCTTAATATTTGCATCAGA+TGG 0.445890 1_3:+68110634 Msa0136280:CDS
AATCGAAGGGTTCGAAATTG+AGG 0.446755 1_3:-68108975 None:intergenic
AGGAAAATGATTAATAGTAA+AGG 0.455208 1_3:+68108773 None:intergenic
TAAGTGAAGATGAATATTGT+TGG 0.457861 1_3:+68111138 Msa0136280:three_prime_UTR
TTTCTCAATTGCCGTTGCTC+AGG 0.469493 1_3:+68109120 Msa0136280:CDS
TTGCAGGTGTCTAGATCTAT+TGG 0.477815 1_3:+68110475 Msa0136280:intron
GATACTGACTGTGTAAATAA+GGG 0.479550 1_3:-68109261 None:intergenic
AAGAGCATGCTTGGTTCAGT+TGG 0.486918 1_3:+68111054 Msa0136280:CDS
GAATCTGAGAAGAGCATGCT+TGG 0.489881 1_3:+68111045 Msa0136280:CDS
AGCAGCTCTTACTAGTCTCT+TGG 0.490227 1_3:-68110818 None:intergenic
AGGGAACATATCTTTGATTG+AGG 0.491568 1_3:+68108834 Msa0136280:five_prime_UTR
TCTGAATAGCACGGCAAAAG+TGG 0.492113 1_3:-68111645 None:intergenic
TGTGTTGTATGTTGCCAACC+TGG 0.492259 1_3:+68109908 Msa0136280:CDS
AACAAAAGAGAAGAAGGAAA+GGG 0.493048 1_3:+68108815 Msa0136280:five_prime_UTR
GACTTGAAACCTCACGCCTC+CGG 0.498145 1_3:+68109094 Msa0136280:CDS
TTTATAGCCGTGGTGTTTGG+AGG 0.501496 1_3:+68110081 Msa0136280:CDS
GGGTGAATGCCGCGTTTAGA+GGG 0.502983 1_3:-68108869 None:intergenic
CCGTGGTGTTTGGAGGATCA+AGG 0.503122 1_3:+68110088 Msa0136280:CDS
GTCATTACTGGACGCTTCAA+AGG 0.503992 1_3:-68110565 None:intergenic
CACATTGTGGTTTATAGCCG+TGG 0.506241 1_3:+68110071 Msa0136280:CDS
GAGGATCAAGGGCATCATAC+AGG 0.507950 1_3:+68110100 Msa0136280:CDS
GTGCCTCTGACTTTCTCCTC+TGG 0.508030 1_3:+68109065 Msa0136280:CDS
CCCTGATGATTCACACATTG+TGG 0.513642 1_3:+68110058 Msa0136280:CDS
TAAGAAAACAAAAGAGAAGA+AGG 0.516063 1_3:+68108809 Msa0136280:five_prime_UTR
ACTCTAGAGTTGTTCTTGGT+AGG 0.521401 1_3:+68109934 Msa0136280:CDS
GTAGAATTTGCCTCTGAACA+AGG 0.528771 1_3:+68109744 Msa0136280:intron
TAGTCCTCAGATTGCGTCTG+TGG 0.530528 1_3:+68109851 Msa0136280:CDS
CATGCTCTTCTCAGATTCAT+CGG 0.531284 1_3:-68111040 None:intergenic
ATCAGGACCGCATAAGGCCG+TGG 0.531919 1_3:-68111767 None:intergenic
ATACAGAAAATGTGATAAAG+TGG 0.539215 1_3:+68111551 Msa0136280:three_prime_UTR
TCTCCTTCCGCCACCTATGT+CGG 0.544351 1_3:+68109181 Msa0136280:CDS
TCACCTGTGTTTCTTACCAC+AGG 0.546900 1_3:+68111803 Msa0136280:three_prime_UTR
GGAGGCTGTGCGCTTGTCAA+CGG 0.547101 1_3:+68109986 Msa0136280:CDS
AATGATATGGTACGAGGTAA+GGG 0.547720 1_3:+68111386 Msa0136280:three_prime_UTR
TGATGATGGTGATTAGGTAT+CGG 0.548228 1_3:-68108934 None:intergenic
TAGAATTTGCCTCTGAACAA+GGG 0.551971 1_3:+68109745 Msa0136280:intron
GCTTTAGCCGAAACACAAGT+TGG 0.552383 1_3:-68111704 None:intergenic
CCGTGGTGTTTGTTTGAAAG+GGG 0.556077 1_3:-68111750 None:intergenic
CGTGGTGTTTGGAGGATCAA+GGG 0.557389 1_3:+68110089 Msa0136280:CDS
AGTCAGAGGCACCACGGCGA+CGG 0.560686 1_3:-68109054 None:intergenic
CGTGGTGTTTGTTTGAAAGG+GGG 0.565718 1_3:-68111749 None:intergenic
TCTGTATGTGAGAATGGATG+TGG 0.566163 1_3:-68111535 None:intergenic
GAGTTTAAGCAAACTGCTGT+TGG 0.576565 1_3:+68110979 Msa0136280:CDS
ACCATCGTAAACACCGACAT+AGG 0.579030 1_3:-68109194 None:intergenic
AAAAGAAATGGAAAAGTCAG+TGG 0.579546 1_3:-68108890 None:intergenic
GACATAGGTGGCGGAAGGAG+AGG 0.580248 1_3:-68109179 None:intergenic
AATGATTAATAGTAAAGGGA+AGG 0.580559 1_3:+68108778 Msa0136280:five_prime_UTR
GAACAAGAATTCTTCTGTGG+AGG 0.580777 1_3:+68109968 Msa0136280:CDS
AACTCTAGAGTCACCCAGGT+TGG 0.586854 1_3:-68109922 None:intergenic
TTGTCTGCAACTTCATGTCG+TGG 0.591077 1_3:-68111499 None:intergenic
CATCACTTAATTGTTCCCAA+AGG 0.592457 1_3:-68110657 None:intergenic
GGAACATATCTTTGATTGAG+GGG 0.592711 1_3:+68108836 Msa0136280:five_prime_UTR
GGATCATAATGTAGCTGATG+AGG 0.594161 1_3:+68110007 Msa0136280:CDS
TGAGAACAAGAATTCTTCTG+TGG 0.594227 1_3:+68109965 Msa0136280:CDS
TATCTATGTATCGAAATCGA+AGG 0.594881 1_3:-68108989 None:intergenic
TGTGTAAATAAGGGAAAAGA+CGG 0.595113 1_3:-68109252 None:intergenic
GTGATCTATCTTGATCAGCA+TGG 0.596846 1_3:+68110940 Msa0136280:CDS
ATCGTAAACACCGACATAGG+TGG 0.597276 1_3:-68109191 None:intergenic
CAGAAAATGTGATAAAGTGG+TGG 0.598245 1_3:+68111554 Msa0136280:three_prime_UTR
ACCTATGTCGGTGTTTACGA+TGG 0.599872 1_3:+68109193 Msa0136280:CDS
TAATAATTTATAGATGATGA+TGG 0.600551 1_3:-68108948 None:intergenic
GGGAGAATTGAAGCTAGGAA+AGG 0.601553 1_3:-68111729 None:intergenic
GGGAACATATCTTTGATTGA+GGG 0.603886 1_3:+68108835 Msa0136280:five_prime_UTR
GTCGGTGTTTACGATGGTCA+CGG 0.604796 1_3:+68109199 Msa0136280:CDS
ACAAACACCACGGCCTTATG+CGG 0.604808 1_3:+68111760 Msa0136280:three_prime_UTR
CGATAACAGTGATATCGTCA+TGG 0.607261 1_3:-68110918 None:intergenic
GGAGAAAGTCAGAGGCACCA+CGG 0.613215 1_3:-68109060 None:intergenic
ATCTATGTATCGAAATCGAA+GGG 0.613366 1_3:-68108988 None:intergenic
GTTTCAAGTCTGTGTGCCAG+AGG 0.616689 1_3:-68109081 None:intergenic
AACGGCAATTGAGAAATCAC+CGG 0.616907 1_3:-68109113 None:intergenic
CCACAATGTGTGAATCATCA+GGG 0.617006 1_3:-68110058 None:intergenic
ATATGGTACGAGGTAAGGGT+TGG 0.619333 1_3:+68111390 Msa0136280:three_prime_UTR
TTACCACAGGCACAGTCTCG+AGG 0.620337 1_3:+68111816 Msa0136280:three_prime_UTR
AGTCCTCAGATTGCGTCTGT+GGG 0.621414 1_3:+68109852 Msa0136280:CDS
GGTGAATGCCGCGTTTAGAG+GGG 0.622833 1_3:-68108868 None:intergenic
AAAGAAATGGAAAAGTCAGT+GGG 0.624711 1_3:-68108889 None:intergenic
CAACCTCGAGACTGTGCCTG+TGG 0.624722 1_3:-68111819 None:intergenic
GGACAACGAAAGTACGTGGA+TGG 0.627942 1_3:-68111683 None:intergenic
GAGAAATCACCGGAGGCGTG+AGG 0.628233 1_3:-68109103 None:intergenic
GTGCCAGAGGAGAAAGTCAG+AGG 0.628468 1_3:-68109068 None:intergenic
AGAATTTGCCTCTGAACAAG+GGG 0.635083 1_3:+68109746 Msa0136280:intron
GACATAAAGAAAATTGACAA+AGG 0.637095 1_3:+68110880 Msa0136280:CDS
AAAACTGCTTGGTGGGCAGG+TGG 0.639272 1_3:+68111433 Msa0136280:three_prime_UTR
ATGTAGCTGATGAGGAAGTG+AGG 0.643026 1_3:+68110015 Msa0136280:CDS
ACACCGACATAGGTGGCGGA+AGG 0.644257 1_3:-68109184 None:intergenic
ATCCAAACATCTGAATAGCA+CGG 0.645228 1_3:-68111654 None:intergenic
CACAGGCACAGTCTCGAGGT+TGG 0.646521 1_3:+68111820 Msa0136280:three_prime_UTR
GTGTTGTATGTTGCCAACCT+GGG 0.647500 1_3:+68109909 Msa0136280:CDS
GTGCCTGTGGTAAGAAACAC+AGG 0.651716 1_3:-68111806 None:intergenic
AGAGTAATTAGCCTGAGCAA+CGG 0.654865 1_3:-68109131 None:intergenic
GTGAATGCCGCGTTTAGAGG+GGG 0.662612 1_3:-68108867 None:intergenic
GATCCCACAGACGCAATCTG+AGG 0.664939 1_3:-68109855 None:intergenic
GTAAACACCGACATAGGTGG+CGG 0.665590 1_3:-68109188 None:intergenic
CAGCATGGAAGCTCTTCTAG+TGG 0.667037 1_3:+68110955 Msa0136280:CDS
GATGTTTATGATAAGCAGAG+TGG 0.667296 1_3:+68111455 Msa0136280:three_prime_UTR
ATTAGCTATAGATCCATACA+AGG 0.667906 1_3:-68111356 None:intergenic
TAGAGAAAATATATCAACAG+GGG 0.669880 1_3:-68111013 None:intergenic
GAACAACTCTAGAGTCACCC+AGG 0.676212 1_3:-68109926 None:intergenic
AGTTGGACAACGAAAGTACG+TGG 0.681436 1_3:-68111687 None:intergenic
GGCAATTGAGAAATCACCGG+AGG 0.706892 1_3:-68109110 None:intergenic
GGCGTTTAGTAAGACCGAGG+AGG 0.718692 1_3:+68109794 Msa0136280:CDS
TTCGTGAATGATATGGTACG+AGG 0.722011 1_3:+68111380 Msa0136280:three_prime_UTR
TTAGAGAAAATATATCAACA+GGG 0.731235 1_3:-68111014 None:intergenic
CCCCTTTCAAACAAACACCA+CGG 0.736954 1_3:+68111750 Msa0136280:three_prime_UTR
CAATGTGTGAATCATCAGGG+TGG 0.738705 1_3:-68110055 None:intergenic
GAATTTGCCTCTGAACAAGG+GGG 0.739786 1_3:+68109747 Msa0136280:intron
AAAGGCGTTTAGTAAGACCG+AGG 0.745268 1_3:+68109791 Msa0136280:CDS
CCTTGATCCTCCAAACACCA+CGG 0.748053 1_3:-68110088 None:intergenic
GGTGTTTACGATGGTCACGG+TGG 0.770607 1_3:+68109202 Msa0136280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATTTATAGATGATGA+TGG - chr1_3:68108951-68108970 None:intergenic 15.0%
!! TAAATAATAACTACAAGTAA+TGG - chr1_3:68109293-68109312 None:intergenic 15.0%
!! TATTAAGATCTAATTTGATT+TGG + chr1_3:68109410-68109429 Msa0136280:intron 15.0%
!!! GAATAATTTTATCCAAAATA+AGG - chr1_3:68111117-68111136 None:intergenic 15.0%
!! TGAAATGAAATGAAAAGAAA+TGG - chr1_3:68108905-68108924 None:intergenic 20.0%
!! ACATTAGCAAAAAAAAACAT+AGG - chr1_3:68109683-68109702 None:intergenic 20.0%
!! ACAAATATAATATATACCGT+TGG + chr1_3:68110124-68110143 Msa0136280:intron 20.0%
!! ACAAAAAATGTACATAATGA+AGG - chr1_3:68110312-68110331 None:intergenic 20.0%
!! TTAGAGAAAATATATCAACA+GGG - chr1_3:68111017-68111036 None:intergenic 20.0%
!! TTTAGAGAAAATATATCAAC+AGG - chr1_3:68111018-68111037 None:intergenic 20.0%
!!! AGTTGTGAAGTTTATATTTT+TGG + chr1_3:68111267-68111286 Msa0136280:three_prime_UTR 20.0%
!!! GTTGTGAAGTTTATATTTTT+GGG + chr1_3:68111268-68111287 Msa0136280:three_prime_UTR 20.0%
! AATGATTAATAGTAAAGGGA+AGG + chr1_3:68108778-68108797 Msa0136280:five_prime_UTR 25.0%
! TAAGAAAACAAAAGAGAAGA+AGG + chr1_3:68108809-68108828 Msa0136280:five_prime_UTR 25.0%
! ATTGACAGAAAATATAACGA+TGG - chr1_3:68109605-68109624 None:intergenic 25.0%
!! TCGTTATATTTTCTGTCAAT+TGG + chr1_3:68109605-68109624 Msa0136280:intron 25.0%
! AGCAAAAAAAAACATAGGTT+CGG - chr1_3:68109678-68109697 None:intergenic 25.0%
! TAATTGCAAATAAAAACCAC+CGG - chr1_3:68110238-68110257 None:intergenic 25.0%
!!! GTTTATTTTTGCCGATAAAT+GGG + chr1_3:68110416-68110435 Msa0136280:intron 25.0%
!!! TTTATTTTTGCCGATAAATG+GGG + chr1_3:68110417-68110436 Msa0136280:intron 25.0%
! TTCTTAATATTTGCATCAGA+TGG + chr1_3:68110634-68110653 Msa0136280:CDS 25.0%
!! AGAGTTTCAATTTTCTTACA+TGG + chr1_3:68110757-68110776 Msa0136280:intron 25.0%
! GACATAAAGAAAATTGACAA+AGG + chr1_3:68110880-68110899 Msa0136280:CDS 25.0%
! TAGAGAAAATATATCAACAG+GGG - chr1_3:68111016-68111035 None:intergenic 25.0%
! TAAGTGAAGATGAATATTGT+TGG + chr1_3:68111138-68111157 Msa0136280:three_prime_UTR 25.0%
!! TTAATTGATTAGTCCTTGTA+TGG + chr1_3:68111343-68111362 Msa0136280:three_prime_UTR 25.0%
! ATACAGAAAATGTGATAAAG+TGG + chr1_3:68111551-68111570 Msa0136280:three_prime_UTR 25.0%
! GTGACGATAAAAAAAAAATC+AGG - chr1_3:68111787-68111806 None:intergenic 25.0%
AAACAAAAGAGAAGAAGGAA+AGG + chr1_3:68108814-68108833 Msa0136280:five_prime_UTR 30.0%
AACAAAAGAGAAGAAGGAAA+GGG + chr1_3:68108815-68108834 Msa0136280:five_prime_UTR 30.0%
AAAGAAATGGAAAAGTCAGT+GGG - chr1_3:68108892-68108911 None:intergenic 30.0%
AAAAGAAATGGAAAAGTCAG+TGG - chr1_3:68108893-68108912 None:intergenic 30.0%
!! TATAGATGATGATGGTGATT+AGG - chr1_3:68108943-68108962 None:intergenic 30.0%
ATCTATGTATCGAAATCGAA+GGG - chr1_3:68108991-68109010 None:intergenic 30.0%
TATCTATGTATCGAAATCGA+AGG - chr1_3:68108992-68109011 None:intergenic 30.0%
TGTGTAAATAAGGGAAAAGA+CGG - chr1_3:68109255-68109274 None:intergenic 30.0%
! GATACTGACTGTGTAAATAA+GGG - chr1_3:68109264-68109283 None:intergenic 30.0%
! AGATACTGACTGTGTAAATA+AGG - chr1_3:68109265-68109284 None:intergenic 30.0%
!! ACATGTCAATGTGTTTTTGT+TGG + chr1_3:68109553-68109572 Msa0136280:intron 30.0%
CTTTGTTAATAAGAGACCAA+CGG - chr1_3:68110143-68110162 None:intergenic 30.0%
!!! GGTTTATTTTTGCCGATAAA+TGG + chr1_3:68110415-68110434 Msa0136280:intron 30.0%
!!! CAGTTTTTTGTTTATGTTGC+AGG + chr1_3:68110459-68110478 Msa0136280:intron 30.0%
TTTCCAAATTGCCGAAAAAT+GGG - chr1_3:68110538-68110557 None:intergenic 30.0%
ATTTCCAAATTGCCGAAAAA+TGG - chr1_3:68110539-68110558 None:intergenic 30.0%
TGAACCATCAATCATCATTA+GGG + chr1_3:68110591-68110610 Msa0136280:CDS 30.0%
!! ATCATAAGCTGACCTTATTT+TGG + chr1_3:68111102-68111121 Msa0136280:three_prime_UTR 30.0%
TTGGTAAGTTAAGAGAATTC+TGG + chr1_3:68111157-68111176 Msa0136280:three_prime_UTR 30.0%
! AGTTGCTGACATATTTTTCT+TGG + chr1_3:68111225-68111244 Msa0136280:three_prime_UTR 30.0%
ATTAGCTATAGATCCATACA+AGG - chr1_3:68111359-68111378 None:intergenic 30.0%
! ACATTTTCTGTATGTGAGAA+TGG - chr1_3:68111544-68111563 None:intergenic 30.0%
AGGGAACATATCTTTGATTG+AGG + chr1_3:68108834-68108853 Msa0136280:five_prime_UTR 35.0%
GGGAACATATCTTTGATTGA+GGG + chr1_3:68108835-68108854 Msa0136280:five_prime_UTR 35.0%
GGAACATATCTTTGATTGAG+GGG + chr1_3:68108836-68108855 Msa0136280:five_prime_UTR 35.0%
!! TGATGATGGTGATTAGGTAT+CGG - chr1_3:68108937-68108956 None:intergenic 35.0%
TTGACAAATCTAGAAGCTTC+AGG - chr1_3:68109229-68109248 None:intergenic 35.0%
!! TTATTAGTTTGGTGCAGCAT+TGG + chr1_3:68109342-68109361 Msa0136280:intron 35.0%
CACATTGACATGTCAAACAT+GGG - chr1_3:68109546-68109565 None:intergenic 35.0%
ACACATTGACATGTCAAACA+TGG - chr1_3:68109547-68109566 None:intergenic 35.0%
TAGAATTTGCCTCTGAACAA+GGG + chr1_3:68109745-68109764 Msa0136280:intron 35.0%
GTCAGCAGATGTGATAAAAA+AGG + chr1_3:68109773-68109792 Msa0136280:CDS 35.0%
TGAGAACAAGAATTCTTCTG+TGG + chr1_3:68109965-68109984 Msa0136280:CDS 35.0%
! GATTGTGGTGTAATTTTGTC+CGG + chr1_3:68110216-68110235 Msa0136280:intron 35.0%
!! TCTACAGAGATCCCATTTTT+CGG + chr1_3:68110524-68110543 Msa0136280:CDS 35.0%
CTGAACCATCAATCATCATT+AGG + chr1_3:68110590-68110609 Msa0136280:CDS 35.0%
CATCACTTAATTGTTCCCAA+AGG - chr1_3:68110660-68110679 None:intergenic 35.0%
GCTAATGTTCGTGAATGATA+TGG + chr1_3:68111373-68111392 Msa0136280:three_prime_UTR 35.0%
AATGATATGGTACGAGGTAA+GGG + chr1_3:68111386-68111405 Msa0136280:three_prime_UTR 35.0%
ACCAACTAACAAAAACTGCT+TGG + chr1_3:68111422-68111441 Msa0136280:three_prime_UTR 35.0%
! ACCAAGCAGTTTTTGTTAGT+TGG - chr1_3:68111426-68111445 None:intergenic 35.0%
AACTAACAAAAACTGCTTGG+TGG + chr1_3:68111425-68111444 Msa0136280:three_prime_UTR 35.0%
ACTAACAAAAACTGCTTGGT+GGG + chr1_3:68111426-68111445 Msa0136280:three_prime_UTR 35.0%
GATGTTTATGATAAGCAGAG+TGG + chr1_3:68111455-68111474 Msa0136280:three_prime_UTR 35.0%
CAGAAAATGTGATAAAGTGG+TGG + chr1_3:68111554-68111573 Msa0136280:three_prime_UTR 35.0%
ATCCAAACATCTGAATAGCA+CGG - chr1_3:68111657-68111676 None:intergenic 35.0%
AAAAAAATCAGGACCGCATA+AGG - chr1_3:68111776-68111795 None:intergenic 35.0%
AATCGAAGGGTTCGAAATTG+AGG - chr1_3:68108978-68108997 None:intergenic 40.0%
GACGGAAGCAAAAATCAAGA+GGG - chr1_3:68109039-68109058 None:intergenic 40.0%
AACGGCAATTGAGAAATCAC+CGG - chr1_3:68109116-68109135 None:intergenic 40.0%
! AGAGTAATTAGCCTGAGCAA+CGG - chr1_3:68109134-68109153 None:intergenic 40.0%
GTAGAATTTGCCTCTGAACA+AGG + chr1_3:68109744-68109763 Msa0136280:intron 40.0%
AGAATTTGCCTCTGAACAAG+GGG + chr1_3:68109746-68109765 Msa0136280:intron 40.0%
CAAATGCAAAAAGTCCTCCT+CGG - chr1_3:68109811-68109830 None:intergenic 40.0%
!! ACTCTAGAGTTGTTCTTGGT+AGG + chr1_3:68109934-68109953 Msa0136280:CDS 40.0%
GAACAAGAATTCTTCTGTGG+AGG + chr1_3:68109968-68109987 Msa0136280:CDS 40.0%
GGATCATAATGTAGCTGATG+AGG + chr1_3:68110007-68110026 Msa0136280:CDS 40.0%
CCACAATGTGTGAATCATCA+GGG - chr1_3:68110061-68110080 None:intergenic 40.0%
ACCACAATGTGTGAATCATC+AGG - chr1_3:68110062-68110081 None:intergenic 40.0%
TACCAACATGTCTGTGATTG+TGG + chr1_3:68110201-68110220 Msa0136280:intron 40.0%
! TTTTGCCGATAAATGGGGTA+AGG + chr1_3:68110422-68110441 Msa0136280:intron 40.0%
TTTGCCGATAAATGGGGTAA+GGG + chr1_3:68110423-68110442 Msa0136280:intron 40.0%
TTGCAGGTGTCTAGATCTAT+TGG + chr1_3:68110475-68110494 Msa0136280:intron 40.0%
ATGGGATCTCTGTAGAAATC+TGG - chr1_3:68110520-68110539 None:intergenic 40.0%
!! GATCCCATTTTTCGGCAATT+TGG + chr1_3:68110532-68110551 Msa0136280:CDS 40.0%
AGCTCCCTAATGATGATTGA+TGG - chr1_3:68110598-68110617 None:intergenic 40.0%
TATTTGCATCAGATGGCCTT+TGG + chr1_3:68110641-68110660 Msa0136280:CDS 40.0%
ATTTGCATCAGATGGCCTTT+GGG + chr1_3:68110642-68110661 Msa0136280:CDS 40.0%
CATAAGATACTCACAGCTCT+TGG - chr1_3:68110712-68110731 None:intergenic 40.0%
!!! TTTGTTTTTGTTCCACGTGC+AGG + chr1_3:68110789-68110808 Msa0136280:intron 40.0%
!!! TTGTTTTTGTTCCACGTGCA+GGG + chr1_3:68110790-68110809 Msa0136280:intron 40.0%
CGATAACAGTGATATCGTCA+TGG - chr1_3:68110921-68110940 None:intergenic 40.0%
GTGATCTATCTTGATCAGCA+TGG + chr1_3:68110940-68110959 Msa0136280:CDS 40.0%
GAGTTTAAGCAAACTGCTGT+TGG + chr1_3:68110979-68110998 Msa0136280:CDS 40.0%
CATGCTCTTCTCAGATTCAT+CGG - chr1_3:68111043-68111062 None:intergenic 40.0%
TTCGTGAATGATATGGTACG+AGG + chr1_3:68111380-68111399 Msa0136280:three_prime_UTR 40.0%
GAATGATATGGTACGAGGTA+AGG + chr1_3:68111385-68111404 Msa0136280:three_prime_UTR 40.0%
TCTGTATGTGAGAATGGATG+TGG - chr1_3:68111538-68111557 None:intergenic 40.0%
TCGTTGTCCAACTTGTGTTT+CGG + chr1_3:68111697-68111716 Msa0136280:three_prime_UTR 40.0%
CGACGGAAGCAAAAATCAAG+AGG - chr1_3:68109040-68109059 None:intergenic 45.0%
TTTCTCAATTGCCGTTGCTC+AGG + chr1_3:68109120-68109139 Msa0136280:CDS 45.0%
ATCGTAAACACCGACATAGG+TGG - chr1_3:68109194-68109213 None:intergenic 45.0%
! ACCTATGTCGGTGTTTACGA+TGG + chr1_3:68109193-68109212 Msa0136280:CDS 45.0%
ACCATCGTAAACACCGACAT+AGG - chr1_3:68109197-68109216 None:intergenic 45.0%
ACACCAACACCTCTTATCGA+AGG + chr1_3:68109483-68109502 Msa0136280:intron 45.0%
GAATTTGCCTCTGAACAAGG+GGG + chr1_3:68109747-68109766 Msa0136280:intron 45.0%
!! AAAGGCGTTTAGTAAGACCG+AGG + chr1_3:68109791-68109810 Msa0136280:CDS 45.0%
! CGAGGAGGACTTTTTGCATT+TGG + chr1_3:68109809-68109828 Msa0136280:CDS 45.0%
GACGCAATCTGAGGACTAAT+TGG - chr1_3:68109849-68109868 None:intergenic 45.0%
GTGGGATCATGTTGTCTTCT+TGG + chr1_3:68109870-68109889 Msa0136280:CDS 45.0%
TGTGTTGTATGTTGCCAACC+TGG + chr1_3:68109908-68109927 Msa0136280:CDS 45.0%
GTGTTGTATGTTGCCAACCT+GGG + chr1_3:68109909-68109928 Msa0136280:CDS 45.0%
!! GGTGACTCTAGAGTTGTTCT+TGG + chr1_3:68109930-68109949 Msa0136280:CDS 45.0%
ATGTAGCTGATGAGGAAGTG+AGG + chr1_3:68110015-68110034 Msa0136280:CDS 45.0%
CAATGTGTGAATCATCAGGG+TGG - chr1_3:68110058-68110077 None:intergenic 45.0%
CCCTGATGATTCACACATTG+TGG + chr1_3:68110058-68110077 Msa0136280:CDS 45.0%
CACATTGTGGTTTATAGCCG+TGG + chr1_3:68110071-68110090 Msa0136280:CDS 45.0%
TGGTTTATAGCCGTGGTGTT+TGG + chr1_3:68110078-68110097 Msa0136280:CDS 45.0%
TTTATAGCCGTGGTGTTTGG+AGG + chr1_3:68110081-68110100 Msa0136280:CDS 45.0%
CACCACAATCACAGACATGT+TGG - chr1_3:68110206-68110225 None:intergenic 45.0%
! TGTGGTGTAATTTTGTCCGG+TGG + chr1_3:68110219-68110238 Msa0136280:intron 45.0%
GAGGCAAGCTTATACAATGC+TGG - chr1_3:68110357-68110376 None:intergenic 45.0%
TTGCCTCGCTTATCAGTTAG+AGG + chr1_3:68110370-68110389 Msa0136280:intron 45.0%
!! TATCAGTTAGAGGTGCTGCA+AGG + chr1_3:68110380-68110399 Msa0136280:intron 45.0%
!! ATCAGTTAGAGGTGCTGCAA+GGG + chr1_3:68110381-68110400 Msa0136280:intron 45.0%
GCTACCCTTACCCCATTTAT+CGG - chr1_3:68110430-68110449 None:intergenic 45.0%
ACTGGACGCTTCAAAGGAAT+AGG - chr1_3:68110562-68110581 None:intergenic 45.0%
GTCATTACTGGACGCTTCAA+AGG - chr1_3:68110568-68110587 None:intergenic 45.0%
GATGGTTCAGCTGTCATTAC+TGG - chr1_3:68110580-68110599 None:intergenic 45.0%
! AGCAGCTCTTACTAGTCTCT+TGG - chr1_3:68110821-68110840 None:intergenic 45.0%
ACTAGTAAGAGCTGCTCTTC+AGG + chr1_3:68110825-68110844 Msa0136280:CDS 45.0%
! GAATCTGAGAAGAGCATGCT+TGG + chr1_3:68111045-68111064 Msa0136280:CDS 45.0%
! AAGAGCATGCTTGGTTCAGT+TGG + chr1_3:68111054-68111073 Msa0136280:CDS 45.0%
ATATGGTACGAGGTAAGGGT+TGG + chr1_3:68111390-68111409 Msa0136280:three_prime_UTR 45.0%
TTGTCTGCAACTTCATGTCG+TGG - chr1_3:68111502-68111521 None:intergenic 45.0%
TCTGAATAGCACGGCAAAAG+TGG - chr1_3:68111648-68111667 None:intergenic 45.0%
! TGCCGTGCTATTCAGATGTT+TGG + chr1_3:68111652-68111671 Msa0136280:three_prime_UTR 45.0%
! AGTTGGACAACGAAAGTACG+TGG - chr1_3:68111690-68111709 None:intergenic 45.0%
GCTTTAGCCGAAACACAAGT+TGG - chr1_3:68111707-68111726 None:intergenic 45.0%
GGGAGAATTGAAGCTAGGAA+AGG - chr1_3:68111732-68111751 None:intergenic 45.0%
AAAGGGGGAGAATTGAAGCT+AGG - chr1_3:68111737-68111756 None:intergenic 45.0%
CGTGGTGTTTGTTTGAAAGG+GGG - chr1_3:68111752-68111771 None:intergenic 45.0%
CCGTGGTGTTTGTTTGAAAG+GGG - chr1_3:68111753-68111772 None:intergenic 45.0%
CCCCTTTCAAACAAACACCA+CGG + chr1_3:68111750-68111769 Msa0136280:three_prime_UTR 45.0%
GCCGTGGTGTTTGTTTGAAA+GGG - chr1_3:68111754-68111773 None:intergenic 45.0%
TCACCTGTGTTTCTTACCAC+AGG + chr1_3:68111803-68111822 Msa0136280:three_prime_UTR 45.0%
!!! TTTTTTTTTTTTTATTAGTT+TGG + chr1_3:68109331-68109350 Msa0136280:intron 5.0%
! GTTTCAAGTCTGTGTGCCAG+AGG - chr1_3:68109084-68109103 None:intergenic 50.0%
GGCAATTGAGAAATCACCGG+AGG - chr1_3:68109113-68109132 None:intergenic 50.0%
GTAAACACCGACATAGGTGG+CGG - chr1_3:68109191-68109210 None:intergenic 50.0%
! GTCGGTGTTTACGATGGTCA+CGG + chr1_3:68109199-68109218 Msa0136280:CDS 50.0%
! ACGCCTTCGATAAGAGGTGT+TGG - chr1_3:68109489-68109508 None:intergenic 50.0%
TGACAATCCCCCTTGTTCAG+AGG - chr1_3:68109757-68109776 None:intergenic 50.0%
TAGTCCTCAGATTGCGTCTG+TGG + chr1_3:68109851-68109870 Msa0136280:CDS 50.0%
AGTCCTCAGATTGCGTCTGT+GGG + chr1_3:68109852-68109871 Msa0136280:CDS 50.0%
!! AACTCTAGAGTCACCCAGGT+TGG - chr1_3:68109925-68109944 None:intergenic 50.0%
!! GAACAACTCTAGAGTCACCC+AGG - chr1_3:68109929-68109948 None:intergenic 50.0%
CCTTGATCCTCCAAACACCA+CGG - chr1_3:68110091-68110110 None:intergenic 50.0%
CGTGGTGTTTGGAGGATCAA+GGG + chr1_3:68110089-68110108 Msa0136280:CDS 50.0%
!! GAGGATCAAGGGCATCATAC+AGG + chr1_3:68110100-68110119 Msa0136280:CDS 50.0%
GCACCTCTAACTGATAAGCG+AGG - chr1_3:68110376-68110395 None:intergenic 50.0%
!! TCAGTTAGAGGTGCTGCAAG+GGG + chr1_3:68110382-68110401 Msa0136280:intron 50.0%
CAGCATGGAAGCTCTTCTAG+TGG + chr1_3:68110955-68110974 Msa0136280:CDS 50.0%
! ACAAAAACTGCTTGGTGGGC+AGG + chr1_3:68111430-68111449 Msa0136280:three_prime_UTR 50.0%
! GGACAACGAAAGTACGTGGA+TGG - chr1_3:68111686-68111705 None:intergenic 50.0%
GGCCGTGGTGTTTGTTTGAA+AGG - chr1_3:68111755-68111774 None:intergenic 50.0%
! ACAAACACCACGGCCTTATG+CGG + chr1_3:68111760-68111779 Msa0136280:three_prime_UTR 50.0%
GTGCCTGTGGTAAGAAACAC+AGG - chr1_3:68111809-68111828 None:intergenic 50.0%
GTGAATGCCGCGTTTAGAGG+GGG - chr1_3:68108870-68108889 None:intergenic 55.0%
GGTGAATGCCGCGTTTAGAG+GGG - chr1_3:68108871-68108890 None:intergenic 55.0%
GGGTGAATGCCGCGTTTAGA+GGG - chr1_3:68108872-68108891 None:intergenic 55.0%
TGGGTGAATGCCGCGTTTAG+AGG - chr1_3:68108873-68108892 None:intergenic 55.0%
! GATTTTTGCTTCCGTCGCCG+TGG + chr1_3:68109043-68109062 Msa0136280:CDS 55.0%
!! GGAGAAAGTCAGAGGCACCA+CGG - chr1_3:68109063-68109082 None:intergenic 55.0%
GTGCCTCTGACTTTCTCCTC+TGG + chr1_3:68109065-68109084 Msa0136280:CDS 55.0%
GTGCCAGAGGAGAAAGTCAG+AGG - chr1_3:68109071-68109090 None:intergenic 55.0%
GACTTGAAACCTCACGCCTC+CGG + chr1_3:68109094-68109113 Msa0136280:CDS 55.0%
TCTCCTTCCGCCACCTATGT+CGG + chr1_3:68109181-68109200 Msa0136280:CDS 55.0%
GGTGTTTACGATGGTCACGG+TGG + chr1_3:68109202-68109221 Msa0136280:CDS 55.0%
CCAGACACGCCTTCGATAAG+AGG - chr1_3:68109495-68109514 None:intergenic 55.0%
! CCTCTTATCGAAGGCGTGTC+TGG + chr1_3:68109492-68109511 Msa0136280:intron 55.0%
! TCGAAGGCGTGTCTGGTGTT+TGG + chr1_3:68109499-68109518 Msa0136280:intron 55.0%
GGCGTTTAGTAAGACCGAGG+AGG + chr1_3:68109794-68109813 Msa0136280:CDS 55.0%
GATCCCACAGACGCAATCTG+AGG - chr1_3:68109858-68109877 None:intergenic 55.0%
CCGTGGTGTTTGGAGGATCA+AGG + chr1_3:68110088-68110107 Msa0136280:CDS 55.0%
!! CAGTTAGAGGTGCTGCAAGG+GGG + chr1_3:68110383-68110402 Msa0136280:intron 55.0%
TCTTGGCAATTCCCTGCACG+TGG - chr1_3:68110804-68110823 None:intergenic 55.0%
! AAAACTGCTTGGTGGGCAGG+TGG + chr1_3:68111433-68111452 Msa0136280:three_prime_UTR 55.0%
TTACCACAGGCACAGTCTCG+AGG + chr1_3:68111816-68111835 Msa0136280:three_prime_UTR 55.0%
CCGCGTTTAGAGGGGGAAGA+CGG - chr1_3:68108863-68108882 None:intergenic 60.0%
CCGTCTTCCCCCTCTAAACG+CGG + chr1_3:68108860-68108879 Msa0136280:five_prime_UTR 60.0%
! GAGAAATCACCGGAGGCGTG+AGG - chr1_3:68109106-68109125 None:intergenic 60.0%
!! GACATAGGTGGCGGAAGGAG+AGG - chr1_3:68109182-68109201 None:intergenic 60.0%
!! ACACCGACATAGGTGGCGGA+AGG - chr1_3:68109187-68109206 None:intergenic 60.0%
GGAGGCTGTGCGCTTGTCAA+CGG + chr1_3:68109986-68110005 Msa0136280:CDS 60.0%
!! GAGGTGCTGCAAGGGGGAAA+TGG + chr1_3:68110389-68110408 Msa0136280:intron 60.0%
GCTGCAAGGGGGAAATGGTC+TGG + chr1_3:68110394-68110413 Msa0136280:intron 60.0%
ATCAGGACCGCATAAGGCCG+TGG - chr1_3:68111770-68111789 None:intergenic 60.0%
CAACCTCGAGACTGTGCCTG+TGG - chr1_3:68111822-68111841 None:intergenic 60.0%
CACAGGCACAGTCTCGAGGT+TGG + chr1_3:68111820-68111839 Msa0136280:three_prime_UTR 60.0%
!! AGTCAGAGGCACCACGGCGA+CGG - chr1_3:68109057-68109076 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 68108775 68111865 68108775 ID=Msa0136280;Name=Msa0136280
chr1_3 mRNA 68108775 68111865 68108775 ID=Msa0136280-mRNA-1;Parent=Msa0136280;Name=Msa0136280-mRNA-1;_AED=0.03;_eAED=0.03;_QI=241|1|1|1|1|1|4|785|379
chr1_3 exon 68108775 68109274 68108775 ID=Msa0136280-mRNA-1:exon:23492;Parent=Msa0136280-mRNA-1
chr1_3 exon 68109748 68110121 68109748 ID=Msa0136280-mRNA-1:exon:23493;Parent=Msa0136280-mRNA-1
chr1_3 exon 68110481 68110717 68110481 ID=Msa0136280-mRNA-1:exon:23494;Parent=Msa0136280-mRNA-1
chr1_3 exon 68110811 68111865 68110811 ID=Msa0136280-mRNA-1:exon:23495;Parent=Msa0136280-mRNA-1
chr1_3 five_prime_UTR 68108775 68109015 68108775 ID=Msa0136280-mRNA-1:five_prime_utr;Parent=Msa0136280-mRNA-1
chr1_3 CDS 68109016 68109274 68109016 ID=Msa0136280-mRNA-1:cds;Parent=Msa0136280-mRNA-1
chr1_3 CDS 68109748 68110121 68109748 ID=Msa0136280-mRNA-1:cds;Parent=Msa0136280-mRNA-1
chr1_3 CDS 68110481 68110717 68110481 ID=Msa0136280-mRNA-1:cds;Parent=Msa0136280-mRNA-1
chr1_3 CDS 68110811 68111080 68110811 ID=Msa0136280-mRNA-1:cds;Parent=Msa0136280-mRNA-1
chr1_3 three_prime_UTR 68111081 68111865 68111081 ID=Msa0136280-mRNA-1:three_prime_utr;Parent=Msa0136280-mRNA-1
Gene Sequence

>Msa0136280

ATGCTGCGTTTGTGCTTAAGCCCTCTTGATTTTTGCTTCCGTCGCCGTGGTGCCTCTGACTTTCTCCTCTGGCACACAGACTTGAAACCTCACGCCTCCGGTGATTTCTCAATTGCCGTTGCTCAGGCTAATTACTCTCTTGAAGATCAAAGTCAAGTCTTCACCTCTCCTTCCGCCACCTATGTCGGTGTTTACGATGGTCACGGTGGTCCTGAAGCTTCTAGATTTGTCAACAACCGTCTTTTCCCTTATTTACACAAATTTGCCTCTGAACAAGGGGGATTGTCAGCAGATGTGATAAAAAAGGCGTTTAGTAAGACCGAGGAGGACTTTTTGCATTTGGTTAAGTTGTCGTTGCCAATTAGTCCTCAGATTGCGTCTGTGGGATCATGTTGTCTTCTTGGTGCAATTTCTAACAATGTGTTGTATGTTGCCAACCTGGGTGACTCTAGAGTTGTTCTTGGTAGGAAGTATCTTGAGAACAAGAATTCTTCTGTGGAGGCTGTGCGCTTGTCAACGGATCATAATGTAGCTGATGAGGAAGTGAGGAGAGAAGTTGAAGCTCTCCACCCTGATGATTCACACATTGTGGTTTATAGCCGTGGTGTTTGGAGGATCAAGGGCATCATACAGGTGTCTAGATCTATTGGTGATGTATATTTGAAGAGACCAGATTTCTACAGAGATCCCATTTTTCGGCAATTTGGAAATCCTATTCCTTTGAAGCGTCCAGTAATGACAGCTGAACCATCAATCATCATTAGGGAGCTTGAGTCAGATGATTTATTCTTAATATTTGCATCAGATGGCCTTTGGGAACAATTAAGTGATGAAGCAGCAGTTGATATTGTTTTCAAATATCCAAGAGCTGGAATTGCCAAGAGACTAGTAAGAGCTGCTCTTCAGGAAGCTGCAAAGAAAAGAGAGATGAGATATGCTGACATAAAGAAAATTGACAAAGGAATTAGAAGACACTTCCATGACGATATCACTGTTATCGTGATCTATCTTGATCAGCATGGAAGCTCTTCTAGTGGTGAGTTTAAGCAAACTGCTGTTGGCTATACAACTGCCCCTGTTGATATATTTTCTCTAAATGCCGATGAATCTGAGAAGAGCATGCTTGGTTCAGTTGGCTAA
Protein sequence

>Msa0136280

MLRLCLSPLDFCFRRRGASDFLLWHTDLKPHASGDFSIAVAQANYSLEDQSQVFTSPSATYVGVYDGHGGPEASRFVNNRLFPYLHKFASEQGGLSADVIKKAFSKTEEDFLHLVKLSLPISPQIASVGSCCLLGAISNNVLYVANLGDSRVVLGRKYLENKNSSVEAVRLSTDHNVADEEVRREVEALHPDDSHIVVYSRGVWRIKGIIQVSRSIGDVYLKRPDFYRDPIFRQFGNPIPLKRPVMTAEPSIIIRELESDDLFLIFASDGLWEQLSDEAAVDIVFKYPRAGIAKRLVRAALQEAAKKREMRYADIKKIDKGIRRHFHDDITVIVIYLDQHGSSSSGEFKQTAVGYTTAPVDIFSLNADESEKSMLGSVG*