AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0179100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179100 MtrunA17_Chr1g0207571 99.208 379 3 0 1 379 1 379 0.0 771
Msa0179100 MtrunA17_Chr1g0213351 58.735 332 127 2 17 340 28 357 2.20e-138 399
Msa0179100 MtrunA17_Chr1g0211251 53.743 374 158 3 2 362 9 380 1.62e-137 397
Msa0179100 MtrunA17_Chr7g0250061 51.459 377 161 3 6 362 1 375 8.29e-135 390
Msa0179100 MtrunA17_Chr8g0371321 55.455 330 137 2 17 338 27 354 4.55e-130 378
Msa0179100 MtrunA17_Chr1g0175381 54.969 322 142 1 18 339 36 354 4.88e-129 375
Msa0179100 MtrunA17_Chr2g0294291 53.538 325 148 2 16 339 30 352 1.33e-123 361
Msa0179100 MtrunA17_Chr7g0263281 54.683 331 140 2 16 338 18 346 2.21e-118 348
Msa0179100 MtrunA17_Chr1g0181901 50.140 357 139 4 20 339 27 381 7.00e-112 332
Msa0179100 MtrunA17_Chr3g0089711 46.875 320 159 5 27 339 33 348 2.84e-94 286
Msa0179100 MtrunA17_Chr3g0089721 46.875 320 159 5 27 339 33 348 4.65e-94 286
Msa0179100 MtrunA17_Chr7g0256751 47.040 321 157 6 27 339 34 349 2.53e-86 268
Msa0179100 MtrunA17_Chr7g0256771 47.744 266 132 4 27 289 35 296 6.60e-75 234
Msa0179100 MtrunA17_Chr3g0089691 43.357 286 152 4 81 360 2 283 2.71e-71 225
Msa0179100 MtrunA17_Chr4g0063111 35.326 368 216 9 20 379 34 387 2.11e-62 208
Msa0179100 MtrunA17_Chr4g0063051 33.437 323 175 10 23 339 22 310 5.39e-47 163
Msa0179100 MtrunA17_Chr7g0256761 44.444 180 79 4 163 337 17 180 8.94e-39 137
Msa0179100 MtrunA17_Chr7g0238501 31.208 298 172 10 61 338 263 547 9.35e-37 140
Msa0179100 MtrunA17_Chr2g0295991 34.241 257 149 6 98 344 237 483 1.34e-36 139
Msa0179100 MtrunA17_Chr4g0067301 31.274 259 156 7 98 344 246 494 1.32e-31 125
Msa0179100 MtrunA17_Chr4g0039061 30.137 292 180 7 61 338 202 483 3.15e-31 124
Msa0179100 MtrunA17_Chr1g0193691 29.085 306 154 5 99 346 592 892 1.17e-30 124
Msa0179100 MtrunA17_Chr4g0031521 31.769 277 147 7 98 338 426 696 4.66e-29 119
Msa0179100 MtrunA17_Chr4g0054811 29.412 306 159 6 95 348 478 778 1.58e-28 117
Msa0179100 MtrunA17_Chr3g0105351 29.930 284 135 9 61 340 94 317 4.18e-26 106
Msa0179100 MtrunA17_Chr3g0105371 29.825 285 136 8 60 340 117 341 2.41e-25 105
Msa0179100 MtrunA17_Chr4g0062951 33.161 193 103 7 95 271 1 183 6.73e-25 100
Msa0179100 MtrunA17_Chr1g0212961 29.412 289 133 9 61 346 126 346 1.18e-24 103
Msa0179100 MtrunA17_Chr5g0430481 28.269 283 141 8 61 340 154 377 1.48e-23 100
Msa0179100 MtrunA17_Chr1g0150451 29.617 287 133 9 63 344 110 332 2.56e-21 93.6
Msa0179100 MtrunA17_Chr3g0112291 26.453 344 190 13 29 345 78 385 1.09e-20 92.8
Msa0179100 MtrunA17_Chr4g0070871 30.453 243 113 8 57 286 249 448 1.38e-20 93.2
Msa0179100 MtrunA17_Chr3g0126511 28.163 245 125 7 46 283 106 306 1.56e-20 92.0
Msa0179100 MtrunA17_Chr3g0135961 28.947 304 153 12 54 337 279 539 3.76e-20 92.0
Msa0179100 MtrunA17_Chr3g0136711 29.252 294 140 10 63 352 67 296 4.54e-20 89.7
Msa0179100 MtrunA17_Chr1g0194191 29.316 307 139 11 54 343 112 357 1.43e-19 89.4
Msa0179100 MtrunA17_Chr2g0293811 28.819 288 151 9 54 337 94 331 3.36e-19 88.2
Msa0179100 MtrunA17_Chr5g0407161 27.986 293 135 13 57 339 121 347 1.51e-17 83.2
Msa0179100 MtrunA17_Chr4g0050381 28.322 286 139 10 36 306 32 266 3.29e-17 80.9
Msa0179100 MtrunA17_Chr4g0001761 27.113 284 131 9 46 284 35 287 3.81e-17 82.0
Msa0179100 MtrunA17_Chr3g0136811 26.316 285 132 9 46 284 35 287 6.34e-17 81.3
Msa0179100 MtrunA17_Chr5g0435721 28.017 232 124 6 61 284 223 419 1.09e-16 81.3
Msa0179100 MtrunA17_Chr2g0317021 27.500 280 130 10 64 337 120 332 1.71e-15 76.6
Msa0179100 MtrunA17_Chr1g0150071 27.341 267 126 7 58 284 49 287 2.60e-15 76.6
Msa0179100 MtrunA17_Chr5g0426141 25.974 308 155 11 36 335 37 279 4.54e-15 74.7
Msa0179100 MtrunA17_Chr1g0171571 27.273 297 144 11 52 337 409 644 5.43e-15 76.6
Msa0179100 MtrunA17_Chr1g0158781 28.390 236 127 5 61 294 145 340 7.23e-15 75.5
Msa0179100 MtrunA17_Chr4g0051661 25.952 289 139 11 31 300 149 381 1.22e-14 74.7
Msa0179100 MtrunA17_Chr2g0279151 25.733 307 157 12 36 335 36 278 1.81e-14 73.2
Msa0179100 MtrunA17_Chr3g0144111 27.273 286 117 12 57 335 24 225 3.28e-14 72.0
Msa0179100 MtrunA17_Chr8g0360351 25.965 285 155 11 28 302 29 267 9.09e-14 71.2
Msa0179100 MtrunA17_Chr3g0127091 27.755 245 137 7 60 284 88 312 2.07e-13 70.9
Msa0179100 MtrunA17_Chr3g0115851 26.433 314 146 13 36 335 37 279 5.88e-13 68.6
Msa0179100 MtrunA17_Chr8g0389921 26.222 225 118 6 61 278 198 381 2.36e-12 68.2
Msa0179100 MtrunA17_Chr4g0022171 24.405 336 191 13 54 360 89 390 2.44e-12 68.2
Msa0179100 MtrunA17_Chr6g0483411 24.138 319 165 11 46 340 56 321 8.08e-12 65.9
Msa0179100 MtrunA17_Chr2g0295401 26.073 303 155 13 62 337 93 353 1.11e-11 65.5
Msa0179100 MtrunA17_Chr7g0221691 33.129 163 85 6 142 301 245 386 1.30e-11 65.9
Msa0179100 MtrunA17_Chr7g0272061 25.949 316 150 16 61 337 91 361 4.88e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179100 AT4G33920.1 70.712 379 109 2 1 378 1 378 0.0 549
Msa0179100 AT4G33920.2 70.169 295 86 2 85 378 2 295 3.01e-147 419
Msa0179100 AT3G51370.1 54.648 355 148 3 1 344 8 360 4.66e-140 404
Msa0179100 AT3G51370.3 54.648 355 148 3 1 344 8 360 4.66e-140 404
Msa0179100 AT5G66080.1 56.196 347 141 2 16 353 28 372 1.10e-139 403
Msa0179100 AT3G12620.2 57.270 337 134 2 17 345 31 365 2.37e-135 392
Msa0179100 AT3G12620.1 57.270 337 134 2 17 345 31 365 2.37e-135 392
Msa0179100 AT4G33920.3 71.053 266 75 2 114 378 1 265 9.38e-134 383
Msa0179100 AT3G55050.1 57.447 329 130 2 17 337 32 358 2.72e-133 387
Msa0179100 AT3G55050.2 57.447 329 130 2 17 337 32 358 2.72e-133 387
Msa0179100 AT4G38520.2 53.955 354 153 2 17 362 29 380 1.31e-131 383
Msa0179100 AT4G38520.1 53.955 354 153 2 17 362 29 380 1.31e-131 383
Msa0179100 AT5G06750.3 57.053 319 136 1 22 339 43 361 3.84e-129 376
Msa0179100 AT5G06750.1 57.053 319 136 1 22 339 43 361 3.84e-129 376
Msa0179100 AT3G17090.1 54.985 331 144 3 22 351 45 371 5.56e-128 373
Msa0179100 AT5G02760.1 53.799 329 140 3 20 340 23 347 3.89e-117 345
Msa0179100 AT3G51370.2 55.797 276 114 3 74 344 3 275 2.65e-109 322
Msa0179100 AT5G66080.2 56.767 266 113 1 88 353 5 268 6.25e-109 321
Msa0179100 AT5G06750.2 57.200 250 106 1 91 339 30 279 3.36e-98 295
Msa0179100 AT3G17090.2 52.963 270 123 2 22 290 45 311 3.75e-98 295
Msa0179100 AT3G16560.1 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0179100 AT3G16560.4 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0179100 AT3G16560.2 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0179100 AT5G02400.1 31.884 276 148 6 98 338 396 666 5.92e-33 130
Msa0179100 AT2G46920.3 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0179100 AT2G46920.1 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0179100 AT2G46920.2 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0179100 AT2G46920.4 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0179100 AT2G35350.1 28.328 293 157 5 93 337 483 770 9.13e-32 127
Msa0179100 AT2G28890.1 31.317 281 150 6 96 338 371 646 3.61e-31 125
Msa0179100 AT3G09400.1 32.090 268 144 7 100 338 384 642 1.25e-30 124
Msa0179100 AT1G07630.1 31.159 276 150 5 98 338 384 654 1.40e-30 123
Msa0179100 AT2G40180.1 30.142 282 135 9 61 337 161 385 4.23e-28 114
Msa0179100 AT1G67820.2 31.461 267 128 7 60 316 114 335 7.03e-28 113
Msa0179100 AT1G67820.1 31.461 267 128 7 60 316 150 371 9.99e-28 114
Msa0179100 AT3G16560.3 33.641 217 128 4 98 306 245 453 5.57e-26 108
Msa0179100 AT1G07160.1 29.000 300 148 9 46 337 134 376 9.23e-26 107
Msa0179100 AT2G30020.1 30.605 281 133 8 63 339 172 394 4.36e-23 100
Msa0179100 AT3G09400.2 29.851 268 145 10 100 337 384 638 7.20e-22 97.8
Msa0179100 AT4G31750.2 31.048 248 121 8 63 305 66 268 1.62e-21 94.4
Msa0179100 AT4G31750.1 31.048 248 121 8 63 305 66 268 1.63e-21 94.4
Msa0179100 AT4G31750.3 31.048 248 121 8 63 305 66 268 1.63e-21 94.4
Msa0179100 AT1G43900.1 29.893 281 129 10 61 338 154 369 9.56e-21 93.2
Msa0179100 AT1G07430.1 28.520 277 145 9 25 284 114 354 3.60e-20 92.0
Msa0179100 AT2G33700.1 28.767 292 139 11 51 335 113 342 4.73e-19 88.2
Msa0179100 AT2G29380.1 27.124 306 156 12 54 337 100 360 3.48e-18 85.5
Msa0179100 AT5G10740.1 27.536 276 133 8 63 335 66 277 9.10e-18 84.3
Msa0179100 AT5G10740.2 27.536 276 133 8 63 335 66 277 9.10e-18 84.3
Msa0179100 AT1G48040.1 29.730 296 152 10 54 343 110 355 2.35e-17 83.2
Msa0179100 AT3G62260.3 30.315 254 137 10 57 304 24 243 3.16e-17 81.6
Msa0179100 AT5G53140.1 26.531 294 148 10 63 353 134 362 5.73e-17 82.4
Msa0179100 AT5G53140.2 26.531 294 148 10 63 353 134 362 5.73e-17 82.4
Msa0179100 AT2G25620.1 26.408 284 154 11 57 337 125 356 6.23e-17 82.0
Msa0179100 AT2G25620.2 26.408 284 154 11 57 337 125 356 6.23e-17 82.0
Msa0179100 AT4G26080.1 29.153 295 153 13 58 337 169 422 8.14e-17 82.0
Msa0179100 AT3G62260.1 30.315 254 137 10 57 304 113 332 1.01e-16 81.3
Msa0179100 AT5G51760.2 28.099 242 121 9 61 283 114 321 1.07e-16 81.3
Msa0179100 AT3G62260.2 30.315 254 137 10 57 304 114 333 1.20e-16 81.3
Msa0179100 AT5G24940.1 27.536 276 133 8 63 335 66 277 1.34e-16 81.3
Msa0179100 AT5G51760.1 28.099 242 121 9 61 283 144 351 1.38e-16 81.3
Msa0179100 AT5G59220.1 28.333 240 125 8 54 284 141 342 1.53e-16 80.9
Msa0179100 AT1G18030.1 26.990 289 146 10 43 303 83 334 2.15e-15 77.0
Msa0179100 AT4G31860.3 25.818 275 120 11 58 284 49 287 3.55e-15 75.9
Msa0179100 AT4G31860.1 25.818 275 120 11 58 284 49 287 6.78e-15 75.9
Msa0179100 AT3G51470.2 25.168 298 150 10 47 335 48 281 7.40e-15 75.1
Msa0179100 AT3G51470.1 25.168 298 150 10 47 335 92 325 1.12e-14 75.1
Msa0179100 AT1G78200.1 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0179100 AT1G78200.3 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0179100 AT1G78200.2 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0179100 AT3G16800.2 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0179100 AT3G16800.1 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0179100 AT3G16800.3 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0179100 AT5G57050.1 25.597 293 167 11 58 337 157 411 2.96e-14 74.3
Msa0179100 AT5G57050.2 25.597 293 167 11 58 337 117 371 3.10e-14 73.9
Msa0179100 AT4G08260.1 26.715 277 116 8 63 339 21 210 4.62e-14 71.2
Msa0179100 AT2G40860.1 28.369 282 137 10 63 337 425 648 9.96e-14 73.2
Msa0179100 AT2G40860.4 28.369 282 137 10 63 337 327 550 9.98e-14 73.2
Msa0179100 AT4G28400.3 27.309 249 129 10 64 306 48 250 1.83e-13 70.5
Msa0179100 AT3G16800.4 28.571 252 124 11 98 337 46 253 1.87e-13 70.1
Msa0179100 AT3G16800.6 28.571 252 124 11 98 337 46 253 1.87e-13 70.1
Msa0179100 AT4G31860.2 26.236 263 110 11 58 272 49 275 2.61e-13 70.1
Msa0179100 AT4G28400.1 27.309 249 129 10 64 306 70 272 2.69e-13 70.1
Msa0179100 AT2G25070.1 26.277 274 118 12 59 284 50 287 3.85e-13 70.5
Msa0179100 AT2G25070.2 26.277 274 118 12 59 284 50 287 3.85e-13 70.5
Msa0179100 AT2G40860.2 27.823 248 136 7 63 303 425 636 4.74e-13 70.9
Msa0179100 AT5G26010.1 26.441 295 174 10 52 337 64 324 5.12e-13 69.7
Msa0179100 AT1G22280.3 26.855 283 153 11 36 306 36 276 9.38e-13 68.6
Msa0179100 AT1G03590.1 26.361 349 186 14 54 371 83 391 2.13e-12 68.6
Msa0179100 AT1G18030.2 27.200 250 130 9 43 273 83 299 2.20e-12 67.8
Msa0179100 AT5G01700.1 26.531 294 169 11 61 339 29 290 2.38e-12 67.8
Msa0179100 AT5G01700.2 26.531 294 169 11 61 339 78 339 2.59e-12 68.2
Msa0179100 AT1G78200.5 26.996 263 138 11 54 306 9 227 3.36e-12 66.2
Msa0179100 AT1G78200.4 26.996 263 138 11 54 306 9 227 3.36e-12 66.2
Msa0179100 AT1G17550.1 25.472 318 158 13 56 337 227 501 8.26e-12 67.0
Msa0179100 AT1G17550.2 25.472 318 158 13 56 337 227 501 8.26e-12 67.0
Msa0179100 AT4G27800.3 27.711 249 136 9 58 284 85 311 1.09e-11 65.9
Msa0179100 AT4G27800.2 27.711 249 136 9 58 284 85 311 1.24e-11 65.9
Msa0179100 AT1G72770.4 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0179100 AT1G72770.1 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0179100 AT1G72770.3 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0179100 AT2G34740.1 23.642 313 156 10 36 335 90 332 1.60e-11 65.5
Msa0179100 AT4G27800.1 27.106 273 155 9 58 308 85 335 1.95e-11 65.5

Find 145 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATAAGCTGACCTTATTT+TGG 0.149456 1_4:-66511184 Msa0179100:three_prime_UTR
GTGGGATCATGTTGTCTTCT+TGG 0.195703 1_4:-66512416 Msa0179100:CDS
AGATACTGACTGTGTAAATA+AGG 0.264924 1_4:+66513024 None:intergenic
GTTGGTAAGTTAAGAGAATT+CGG 0.269624 1_4:-66511130 Msa0179100:three_prime_UTR
TGCCGTGCTATTCAGATGTT+TGG 0.272533 1_4:-66510634 Msa0179100:three_prime_UTR
ACTAGTAAGAGCTGCTCTTC+AGG 0.291775 1_4:-66511461 Msa0179100:CDS
GATGGTTCAGCTGTCATTAC+TGG 0.293354 1_4:+66511709 None:intergenic
CGCTAATGTTCGTGAATGTT+AGG 0.296140 1_4:-66510914 Msa0179100:three_prime_UTR
CTGAACCATCAATCATCATT+AGG 0.315695 1_4:-66511696 Msa0179100:CDS
GACGCAATCTGAGGACTAAT+TGG 0.330240 1_4:+66512440 None:intergenic
GGTGACTCTAGAGTTGTTCT+TGG 0.332422 1_4:-66512356 Msa0179100:CDS
TATAGATGATGATGGTGATT+AGG 0.354348 1_4:+66513346 None:intergenic
TATTTGCATCAGATGGCCTT+TGG 0.354565 1_4:-66511645 Msa0179100:CDS
TTAATTGATTAGTCCTTGTA+TGG 0.354606 1_4:-66510943 Msa0179100:three_prime_UTR
TCGTTGTCCAACGTGTGTTT+CGG 0.359086 1_4:-66510589 Msa0179100:three_prime_UTR
TTGACAAATCTAGAAGCTTC+AGG 0.361584 1_4:+66513060 None:intergenic
TTTAGAGAAAATATATCAAC+AGG 0.367089 1_4:+66511271 None:intergenic
TTGGTAAGTTAAGAGAATTC+GGG 0.377389 1_4:-66511129 Msa0179100:three_prime_UTR
AAACAAAAGAGAAGAAGGAA+AGG 0.384883 1_4:-66513472 Msa0179100:five_prime_UTR
ATTTGCATCAGATGGCCTTT+GGG 0.407098 1_4:-66511644 Msa0179100:CDS
ACTGGACGCTTCAAAGGAAT+AGG 0.409207 1_4:+66511727 None:intergenic
TGGGTGAATGCCGCGTTTAG+AGG 0.409840 1_4:+66513416 None:intergenic
TGAAATGAAATGAAATGAAA+TGG 0.412051 1_4:+66513384 None:intergenic
GGAAAATGATTAATAGTAAA+GGG 0.415664 1_4:-66513512 Msa0179100:five_prime_UTR
ATGGGATCTCTGTAGAAATC+TGG 0.417500 1_4:+66511769 None:intergenic
GCCATGGTGTTTGTTTGAAA+GGG 0.419608 1_4:+66510535 None:intergenic
CATAAGACACTCACAGCTCT+TGG 0.419984 1_4:+66511577 None:intergenic
ACCACAATGTGTGAATCATC+AGG 0.421762 1_4:+66512227 None:intergenic
AGCTCCCTAATGATGATTGA+TGG 0.423360 1_4:+66511691 None:intergenic
TGGTTTATAGCCGTGGTGTT+TGG 0.426614 1_4:-66512208 Msa0179100:CDS
TGAACCATCAATCATCATTA+GGG 0.429934 1_4:-66511695 Msa0179100:CDS
GAATGTTAGGGTACGAGGTA+AGG 0.439641 1_4:-66510901 Msa0179100:three_prime_UTR
GGCCATGGTGTTTGTTTGAA+AGG 0.444787 1_4:+66510534 None:intergenic
TTCTTAATATTTGCATCAGA+TGG 0.445890 1_4:-66511652 Msa0179100:CDS
AATCGAAGGGTTCGAAATTG+AGG 0.446755 1_4:+66513311 None:intergenic
AGGAAAATGATTAATAGTAA+AGG 0.455208 1_4:-66513513 Msa0179100:five_prime_UTR
TAAGTGAAGATGAATATTGT+TGG 0.457861 1_4:-66511148 Msa0179100:three_prime_UTR
TTTCTCAATTGCCGTTGCTC+AGG 0.469493 1_4:-66513166 Msa0179100:CDS
TTGCAGGTGTCTAGATCTAT+TGG 0.477815 1_4:-66511811 Msa0179100:intron
GATACTGACTGTGTAAATAA+GGG 0.479550 1_4:+66513025 None:intergenic
TAAGCAGAGTGGTATATTGA+TGG 0.482587 1_4:-66510820 Msa0179100:three_prime_UTR
AAGAGCATGCTTGGTTCAGT+TGG 0.486918 1_4:-66511232 Msa0179100:CDS
GAATCTGAGAAGAGCATGCT+TGG 0.489881 1_4:-66511241 Msa0179100:CDS
GTGCCTCTGACTTCCTCCTC+TGG 0.490211 1_4:-66513221 Msa0179100:CDS
AGCAGCTCTTACTAGTCTCT+TGG 0.490227 1_4:+66511468 None:intergenic
AGGGAACATATCTTTGATTG+AGG 0.491568 1_4:-66513452 Msa0179100:five_prime_UTR
TCTGAATAGCACGGCAAAAG+TGG 0.492113 1_4:+66510641 None:intergenic
AACAAAAGAGAAGAAGGAAA+GGG 0.493048 1_4:-66513471 Msa0179100:five_prime_UTR
TGTGCTGTATGTTGCCAACC+TGG 0.496291 1_4:-66512378 Msa0179100:CDS
GACTTGAAACCTCACGCCTC+CGG 0.498145 1_4:-66513192 Msa0179100:CDS
TTTATAGCCGTGGTGTTTGG+AGG 0.501496 1_4:-66512205 Msa0179100:CDS
GGGTGAATGCCGCGTTTAGA+GGG 0.502983 1_4:+66513417 None:intergenic
CCGTGGTGTTTGGAGGATCA+AGG 0.503122 1_4:-66512198 Msa0179100:CDS
GTCATTACTGGACGCTTCAA+AGG 0.503992 1_4:+66511721 None:intergenic
CACATTGTGGTTTATAGCCG+TGG 0.506241 1_4:-66512215 Msa0179100:CDS
GCTAATGTTCGTGAATGTTA+GGG 0.506360 1_4:-66510913 Msa0179100:three_prime_UTR
GAGGATCAAGGGCATCATAC+AGG 0.507950 1_4:-66512186 Msa0179100:intron
CCCTGATGATTCACACATTG+TGG 0.513642 1_4:-66512228 Msa0179100:CDS
TAAGAAAACAAAAGAGAAGA+AGG 0.516063 1_4:-66513477 Msa0179100:five_prime_UTR
ACTCTAGAGTTGTTCTTGGT+AGG 0.521401 1_4:-66512352 Msa0179100:CDS
GTAGAATTTGCCTCTGAACA+AGG 0.528771 1_4:-66512542 Msa0179100:intron
TAGTCCTCAGATTGCGTCTG+TGG 0.530528 1_4:-66512435 Msa0179100:CDS
CATGCTCTTCTCAGATTCAT+CGG 0.531284 1_4:+66511246 None:intergenic
ATACAGAAAATGTGATAAAG+TGG 0.532184 1_4:-66510735 Msa0179100:three_prime_UTR
TCTGTATCTGAGAATGGATG+TGG 0.538646 1_4:+66510751 None:intergenic
TCTCCTTCCGCCACCTATGT+CGG 0.544351 1_4:-66513105 Msa0179100:CDS
TCACCTGTGTTTCTTACCAC+AGG 0.546900 1_4:-66510482 Msa0179100:three_prime_UTR
GGAGGCTGTGCGCTTGTCAA+CGG 0.547101 1_4:-66512300 Msa0179100:CDS
TGATGATGGTGATTAGGTAT+CGG 0.548228 1_4:+66513352 None:intergenic
AGTCAGAGGCACCACGGCGA+CGG 0.549845 1_4:+66513232 None:intergenic
AAAACTGCTGGGTGGGCAGG+TGG 0.550770 1_4:-66510853 Msa0179100:three_prime_UTR
TAGAATTTGCCTCTGAACAA+GGG 0.551971 1_4:-66512541 Msa0179100:CDS
CCATGGTGTTTGTTTGAAAG+GGG 0.553781 1_4:+66510536 None:intergenic
AATGTTAGGGTACGAGGTAA+GGG 0.555204 1_4:-66510900 Msa0179100:three_prime_UTR
CGTGGTGTTTGGAGGATCAA+GGG 0.557389 1_4:-66512197 Msa0179100:CDS
ATCAGGACCGCATAAGGCCA+TGG 0.560900 1_4:+66510519 None:intergenic
CATGGTGTTTGTTTGAAAGG+GGG 0.574952 1_4:+66510537 None:intergenic
GAGTTTAAGCAAACTGCTGT+TGG 0.576565 1_4:-66511307 Msa0179100:CDS
ACCATCGTAAACACCGACAT+AGG 0.579030 1_4:+66513092 None:intergenic
GACATAGGTGGCGGAAGGAG+AGG 0.580248 1_4:+66513107 None:intergenic
AATGATTAATAGTAAAGGGA+AGG 0.580559 1_4:-66513508 Msa0179100:five_prime_UTR
GAACAAGAATTCTTCTGTGG+AGG 0.580777 1_4:-66512318 Msa0179100:CDS
AACTCTAGAGTCACCCAGGT+TGG 0.586854 1_4:+66512364 None:intergenic
TTGTCTGCAACTTCATGTCG+TGG 0.591077 1_4:+66510787 None:intergenic
CATCACTTAATTGTTCCCAA+AGG 0.592457 1_4:+66511629 None:intergenic
GGAACATATCTTTGATTGAG+GGG 0.592711 1_4:-66513450 Msa0179100:five_prime_UTR
GGATCATAATGTAGCTGATG+AGG 0.594161 1_4:-66512279 Msa0179100:CDS
TGAGAACAAGAATTCTTCTG+TGG 0.594227 1_4:-66512321 Msa0179100:CDS
TATCTATGTATCGAAATCGA+AGG 0.594881 1_4:+66513297 None:intergenic
TGTGTAAATAAGGGAAAAGA+CGG 0.595113 1_4:+66513034 None:intergenic
GTGATCTATCTTGATCAGCA+TGG 0.596846 1_4:-66511346 Msa0179100:CDS
ATCGTAAACACCGACATAGG+TGG 0.597276 1_4:+66513095 None:intergenic
CAGAAAATGTGATAAAGTGG+TGG 0.598245 1_4:-66510732 Msa0179100:three_prime_UTR
ACCTATGTCGGTGTTTACGA+TGG 0.599872 1_4:-66513093 Msa0179100:CDS
TAATAATTTATAGATGATGA+TGG 0.600551 1_4:+66513338 None:intergenic
GGGAGAATTGAAGCTAGGAA+AGG 0.601553 1_4:+66510557 None:intergenic
GTGCCAGAGGAGGAAGTCAG+AGG 0.601693 1_4:+66513218 None:intergenic
GGAGGAAGTCAGAGGCACCA+CGG 0.602149 1_4:+66513226 None:intergenic
GGGAACATATCTTTGATTGA+GGG 0.603886 1_4:-66513451 Msa0179100:five_prime_UTR
TCAAGTCTGTGTGCCAGAGG+AGG 0.604192 1_4:+66513208 None:intergenic
GTCGGTGTTTACGATGGTCA+CGG 0.604796 1_4:-66513087 Msa0179100:CDS
CGATAACAGTGATATCGTCA+TGG 0.607261 1_4:+66511368 None:intergenic
GTTTCAAGTCTGTGTGCCAG+AGG 0.609253 1_4:+66513205 None:intergenic
ATCTATGTATCGAAATCGAA+GGG 0.613366 1_4:+66513298 None:intergenic
AACGGCAATTGAGAAATCAC+CGG 0.616907 1_4:+66513173 None:intergenic
CCACAATGTGTGAATCATCA+GGG 0.617006 1_4:+66512228 None:intergenic
TTAGGGTACGAGGTAAGGGT+TGG 0.619333 1_4:-66510896 Msa0179100:three_prime_UTR
TTACCACAGGCACAGTCTCG+AGG 0.620337 1_4:-66510469 Msa0179100:three_prime_UTR
AGTCCTCAGATTGCGTCTGT+GGG 0.621414 1_4:-66512434 Msa0179100:CDS
GGTGAATGCCGCGTTTAGAG+GGG 0.622833 1_4:+66513418 None:intergenic
CAACCTCGAGACTGTGCCTG+TGG 0.624722 1_4:+66510466 None:intergenic
GAGAAATCACCGGAGGCGTG+AGG 0.628233 1_4:+66513183 None:intergenic
GTGCTGTATGTTGCCAACCT+GGG 0.633140 1_4:-66512377 Msa0179100:CDS
GATGTTTATTATAAGCAGAG+TGG 0.633974 1_4:-66510831 Msa0179100:three_prime_UTR
AGAATTTGCCTCTGAACAAG+GGG 0.635083 1_4:-66512540 Msa0179100:CDS
ACAAACACCATGGCCTTATG+CGG 0.636163 1_4:-66510526 Msa0179100:three_prime_UTR
GACATAAAGAAAATTGACAA+AGG 0.637095 1_4:-66511406 Msa0179100:CDS
TTCGTGAATGTTAGGGTACG+AGG 0.637632 1_4:-66510906 Msa0179100:three_prime_UTR
ATGTAGCTGATGAGGAAGTG+AGG 0.643026 1_4:-66512271 Msa0179100:CDS
ACACCGACATAGGTGGCGGA+AGG 0.644257 1_4:+66513102 None:intergenic
ATCCAAACATCTGAATAGCA+CGG 0.645228 1_4:+66510632 None:intergenic
CACAGGCACAGTCTCGAGGT+TGG 0.646521 1_4:-66510465 Msa0179100:three_prime_UTR
GTGCCTGTGGTAAGAAACAC+AGG 0.651716 1_4:+66510479 None:intergenic
AAATGAAATGGAAAAGTCAG+TGG 0.653608 1_4:+66513396 None:intergenic
AGAGTAATTAGCCTGAGCAA+CGG 0.654865 1_4:+66513155 None:intergenic
CGTTGGACAACGAAAGTACG+TGG 0.662589 1_4:+66510599 None:intergenic
GTGAATGCCGCGTTTAGAGG+GGG 0.662612 1_4:+66513419 None:intergenic
ATTAGCGATAGATCCATACA+AGG 0.663046 1_4:+66510930 None:intergenic
GATCCCACAGACGCAATCTG+AGG 0.664939 1_4:+66512431 None:intergenic
GTAAACACCGACATAGGTGG+CGG 0.665590 1_4:+66513098 None:intergenic
CAGCATGGAAGCTCTTCTAG+TGG 0.667037 1_4:-66511331 Msa0179100:CDS
TAGAGAAAATATATCAACAG+GGG 0.669880 1_4:+66511273 None:intergenic
GCTTTAGCCGAAACACACGT+TGG 0.672655 1_4:+66510582 None:intergenic
GGACAACGAAAGTACGTGGA+CGG 0.674893 1_4:+66510603 None:intergenic
GAACAACTCTAGAGTCACCC+AGG 0.676212 1_4:+66512360 None:intergenic
AATGAAATGGAAAAGTCAGT+GGG 0.679469 1_4:+66513397 None:intergenic
CCCCTTTCAAACAAACACCA+TGG 0.681563 1_4:-66510536 Msa0179100:three_prime_UTR
GGCAATTGAGAAATCACCGG+AGG 0.706892 1_4:+66513176 None:intergenic
GGCGTTTAGTAAGACCGAGG+AGG 0.718692 1_4:-66512492 Msa0179100:CDS
TTAGAGAAAATATATCAACA+GGG 0.731235 1_4:+66511272 None:intergenic
CAATGTGTGAATCATCAGGG+TGG 0.738705 1_4:+66512231 None:intergenic
GAATTTGCCTCTGAACAAGG+GGG 0.739786 1_4:-66512539 Msa0179100:CDS
AAAGGCGTTTAGTAAGACCG+AGG 0.745268 1_4:-66512495 Msa0179100:CDS
CCTTGATCCTCCAAACACCA+CGG 0.748053 1_4:+66512198 None:intergenic
GGTGTTTACGATGGTCACGG+TGG 0.770607 1_4:-66513084 Msa0179100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTTTTTTATTAGTT+TGG - chr1_4:66510996-66511015 Msa0179100:three_prime_UTR 10.0%
!! TAATAATTTATAGATGATGA+TGG + chr1_4:66510618-66510637 None:intergenic 15.0%
!! TAAATAATAACTACAAGTAA+TGG + chr1_4:66510960-66510979 None:intergenic 15.0%
!!! GAATAATTTTATCCAAAATA+AGG + chr1_4:66512784-66512803 None:intergenic 15.0%
!! TGAAATGAAATGAAATGAAA+TGG + chr1_4:66510572-66510591 None:intergenic 20.0%
!! TATTAAGATCCAATTTGATT+TGG - chr1_4:66511075-66511094 Msa0179100:three_prime_UTR 20.0%
!! TCAAAAAATCCAAATCAAAT+TGG + chr1_4:66511087-66511106 None:intergenic 20.0%
!! CAAAAAAAAAAACATAGGTT+CGG + chr1_4:66511343-66511362 None:intergenic 20.0%
!! ATCAGCAAAAAAAAAAACAT+AGG + chr1_4:66511348-66511367 None:intergenic 20.0%
!! ACAAATATAATATATACCGT+TGG - chr1_4:66511791-66511810 Msa0179100:CDS 20.0%
!! ACAAAAAATGTACATAATGA+AGG + chr1_4:66511979-66511998 None:intergenic 20.0%
!! TTAGAGAAAATATATCAACA+GGG + chr1_4:66512684-66512703 None:intergenic 20.0%
!! TTTAGAGAAAATATATCAAC+AGG + chr1_4:66512685-66512704 None:intergenic 20.0%
!!! AGTTGTGAAGTTTATATTTT+TGG - chr1_4:66512934-66512953 Msa0179100:intron 20.0%
!!! GTTGTGAAGTTTATATTTTT+GGG - chr1_4:66512935-66512954 Msa0179100:intron 20.0%
!! TGACGATAAAAAAAAAAATC+AGG + chr1_4:66513454-66513473 None:intergenic 20.0%
! AATGATTAATAGTAAAGGGA+AGG - chr1_4:66510445-66510464 Msa0179100:three_prime_UTR 25.0%
! TAAGAAAACAAAAGAGAAGA+AGG - chr1_4:66510476-66510495 Msa0179100:three_prime_UTR 25.0%
! ATTGACAGAAAATATAACGA+TGG + chr1_4:66511270-66511289 None:intergenic 25.0%
!! TCGTTATATTTTCTGTCAAT+TGG - chr1_4:66511270-66511289 Msa0179100:CDS 25.0%
! TAATTGCAAATAAAAACCAC+CGG + chr1_4:66511904-66511923 None:intergenic 25.0%
!!! GTTTATTTTTGCCGATAAAT+GGG - chr1_4:66512083-66512102 Msa0179100:intron 25.0%
!!! TTTATTTTTGCCGATAAATG+GGG - chr1_4:66512084-66512103 Msa0179100:intron 25.0%
! TTCTTAATATTTGCATCAGA+TGG - chr1_4:66512301-66512320 Msa0179100:CDS 25.0%
!! AGAGTTTCAATTTTCTTACA+TGG - chr1_4:66512424-66512443 Msa0179100:CDS 25.0%
! GACATAAAGAAAATTGACAA+AGG - chr1_4:66512547-66512566 Msa0179100:intron 25.0%
! TAGAGAAAATATATCAACAG+GGG + chr1_4:66512683-66512702 None:intergenic 25.0%
! TAAGTGAAGATGAATATTGT+TGG - chr1_4:66512805-66512824 Msa0179100:intron 25.0%
!! TTAATTGATTAGTCCTTGTA+TGG - chr1_4:66513010-66513029 Msa0179100:intron 25.0%
! ATACAGAAAATGTGATAAAG+TGG - chr1_4:66513218-66513237 Msa0179100:CDS 25.0%
AAACAAAAGAGAAGAAGGAA+AGG - chr1_4:66510481-66510500 Msa0179100:three_prime_UTR 30.0%
AACAAAAGAGAAGAAGGAAA+GGG - chr1_4:66510482-66510501 Msa0179100:three_prime_UTR 30.0%
AATGAAATGGAAAAGTCAGT+GGG + chr1_4:66510559-66510578 None:intergenic 30.0%
AAATGAAATGGAAAAGTCAG+TGG + chr1_4:66510560-66510579 None:intergenic 30.0%
!! TATAGATGATGATGGTGATT+AGG + chr1_4:66510610-66510629 None:intergenic 30.0%
ATCTATGTATCGAAATCGAA+GGG + chr1_4:66510658-66510677 None:intergenic 30.0%
TATCTATGTATCGAAATCGA+AGG + chr1_4:66510659-66510678 None:intergenic 30.0%
TGTGTAAATAAGGGAAAAGA+CGG + chr1_4:66510922-66510941 None:intergenic 30.0%
! GATACTGACTGTGTAAATAA+GGG + chr1_4:66510931-66510950 None:intergenic 30.0%
! AGATACTGACTGTGTAAATA+AGG + chr1_4:66510932-66510951 None:intergenic 30.0%
!! ACATGTCAATGTGTTTTTGT+TGG - chr1_4:66511218-66511237 Msa0179100:exon 30.0%
CTTTGTTAATAAGAGACCAA+CGG + chr1_4:66511810-66511829 None:intergenic 30.0%
!!! GGTTTATTTTTGCCGATAAA+TGG - chr1_4:66512082-66512101 Msa0179100:intron 30.0%
!!! CAGTTTTTTGTTTATGTTGC+AGG - chr1_4:66512126-66512145 Msa0179100:intron 30.0%
TTTCCAAATTGCCGAAAAAT+GGG + chr1_4:66512205-66512224 None:intergenic 30.0%
ATTTCCAAATTGCCGAAAAA+TGG + chr1_4:66512206-66512225 None:intergenic 30.0%
TGAACCATCAATCATCATTA+GGG - chr1_4:66512258-66512277 Msa0179100:CDS 30.0%
!! ATCATAAGCTGACCTTATTT+TGG - chr1_4:66512769-66512788 Msa0179100:intron 30.0%
! GTTGGTAAGTTAAGAGAATT+CGG - chr1_4:66512823-66512842 Msa0179100:intron 30.0%
TTGGTAAGTTAAGAGAATTC+GGG - chr1_4:66512824-66512843 Msa0179100:intron 30.0%
! AGTTGCTGACATATTTTTCT+TGG - chr1_4:66512892-66512911 Msa0179100:intron 30.0%
GATGTTTATTATAAGCAGAG+TGG - chr1_4:66513122-66513141 Msa0179100:CDS 30.0%
! ACATTTTCTGTATCTGAGAA+TGG + chr1_4:66513211-66513230 None:intergenic 30.0%
AGGGAACATATCTTTGATTG+AGG - chr1_4:66510501-66510520 Msa0179100:three_prime_UTR 35.0%
GGGAACATATCTTTGATTGA+GGG - chr1_4:66510502-66510521 Msa0179100:three_prime_UTR 35.0%
GGAACATATCTTTGATTGAG+GGG - chr1_4:66510503-66510522 Msa0179100:three_prime_UTR 35.0%
!! TGATGATGGTGATTAGGTAT+CGG + chr1_4:66510604-66510623 None:intergenic 35.0%
TTGACAAATCTAGAAGCTTC+AGG + chr1_4:66510896-66510915 None:intergenic 35.0%
!! TTATTAGTTTGGTGCAGCAT+TGG - chr1_4:66511007-66511026 Msa0179100:three_prime_UTR 35.0%
CACATTGACATGTCAAACAT+CGG + chr1_4:66511211-66511230 None:intergenic 35.0%
TAGAATTTGCCTCTGAACAA+GGG - chr1_4:66511412-66511431 Msa0179100:CDS 35.0%
GTCAGCAGATGTGATAAAAA+AGG - chr1_4:66511440-66511459 Msa0179100:CDS 35.0%
TGAGAACAAGAATTCTTCTG+TGG - chr1_4:66511632-66511651 Msa0179100:CDS 35.0%
! TGATTGTGGTGTAATTTGTC+CGG - chr1_4:66511882-66511901 Msa0179100:intron 35.0%
!! TCTACAGAGATCCCATTTTT+CGG - chr1_4:66512191-66512210 Msa0179100:CDS 35.0%
CTGAACCATCAATCATCATT+AGG - chr1_4:66512257-66512276 Msa0179100:CDS 35.0%
CATCACTTAATTGTTCCCAA+AGG + chr1_4:66512327-66512346 None:intergenic 35.0%
ATTAGCGATAGATCCATACA+AGG + chr1_4:66513026-66513045 None:intergenic 35.0%
GCTAATGTTCGTGAATGTTA+GGG - chr1_4:66513040-66513059 Msa0179100:CDS 35.0%
ACCAACTAACAAAAACTGCT+GGG - chr1_4:66513089-66513108 Msa0179100:CDS 35.0%
TAAGCAGAGTGGTATATTGA+TGG - chr1_4:66513133-66513152 Msa0179100:CDS 35.0%
CAGAAAATGTGATAAAGTGG+TGG - chr1_4:66513221-66513240 Msa0179100:CDS 35.0%
ATCCAAACATCTGAATAGCA+CGG + chr1_4:66513324-66513343 None:intergenic 35.0%
AAAAAAATCAGGACCGCATA+AGG + chr1_4:66513443-66513462 None:intergenic 35.0%
AATCGAAGGGTTCGAAATTG+AGG + chr1_4:66510645-66510664 None:intergenic 40.0%
GACGGAAGCAAAAATCAAGA+GGG + chr1_4:66510706-66510725 None:intergenic 40.0%
AACGGCAATTGAGAAATCAC+CGG + chr1_4:66510783-66510802 None:intergenic 40.0%
! AGAGTAATTAGCCTGAGCAA+CGG + chr1_4:66510801-66510820 None:intergenic 40.0%
GTAGAATTTGCCTCTGAACA+AGG - chr1_4:66511411-66511430 Msa0179100:CDS 40.0%
AGAATTTGCCTCTGAACAAG+GGG - chr1_4:66511413-66511432 Msa0179100:CDS 40.0%
CAAATGCAAAAAGTCCTCCT+CGG + chr1_4:66511478-66511497 None:intergenic 40.0%
!! ACTCTAGAGTTGTTCTTGGT+AGG - chr1_4:66511601-66511620 Msa0179100:CDS 40.0%
GAACAAGAATTCTTCTGTGG+AGG - chr1_4:66511635-66511654 Msa0179100:CDS 40.0%
GGATCATAATGTAGCTGATG+AGG - chr1_4:66511674-66511693 Msa0179100:CDS 40.0%
CCACAATGTGTGAATCATCA+GGG + chr1_4:66511728-66511747 None:intergenic 40.0%
ACCACAATGTGTGAATCATC+AGG + chr1_4:66511729-66511748 None:intergenic 40.0%
TACCAACATGTCTGTGATTG+TGG - chr1_4:66511868-66511887 Msa0179100:intron 40.0%
! TTTTGCCGATAAATGGGGTA+AGG - chr1_4:66512089-66512108 Msa0179100:intron 40.0%
TTTGCCGATAAATGGGGTAA+GGG - chr1_4:66512090-66512109 Msa0179100:intron 40.0%
TTGCAGGTGTCTAGATCTAT+TGG - chr1_4:66512142-66512161 Msa0179100:intron 40.0%
ATGGGATCTCTGTAGAAATC+TGG + chr1_4:66512187-66512206 None:intergenic 40.0%
!! GATCCCATTTTTCGGCAATT+TGG - chr1_4:66512199-66512218 Msa0179100:CDS 40.0%
AGCTCCCTAATGATGATTGA+TGG + chr1_4:66512265-66512284 None:intergenic 40.0%
TATTTGCATCAGATGGCCTT+TGG - chr1_4:66512308-66512327 Msa0179100:CDS 40.0%
ATTTGCATCAGATGGCCTTT+GGG - chr1_4:66512309-66512328 Msa0179100:CDS 40.0%
!!! TTTGTTTTTGTTCCACGTGC+AGG - chr1_4:66512456-66512475 Msa0179100:CDS 40.0%
!!! TTGTTTTTGTTCCACGTGCA+GGG - chr1_4:66512457-66512476 Msa0179100:CDS 40.0%
CGATAACAGTGATATCGTCA+TGG + chr1_4:66512588-66512607 None:intergenic 40.0%
GTGATCTATCTTGATCAGCA+TGG - chr1_4:66512607-66512626 Msa0179100:intron 40.0%
GAGTTTAAGCAAACTGCTGT+TGG - chr1_4:66512646-66512665 Msa0179100:intron 40.0%
CATGCTCTTCTCAGATTCAT+CGG + chr1_4:66512710-66512729 None:intergenic 40.0%
CGCTAATGTTCGTGAATGTT+AGG - chr1_4:66513039-66513058 Msa0179100:CDS 40.0%
AATGTTAGGGTACGAGGTAA+GGG - chr1_4:66513053-66513072 Msa0179100:CDS 40.0%
CACCAACTAACAAAAACTGC+TGG - chr1_4:66513088-66513107 Msa0179100:CDS 40.0%
! ACCCAGCAGTTTTTGTTAGT+TGG + chr1_4:66513093-66513112 None:intergenic 40.0%
AACTAACAAAAACTGCTGGG+TGG - chr1_4:66513092-66513111 Msa0179100:CDS 40.0%
ACTAACAAAAACTGCTGGGT+GGG - chr1_4:66513093-66513112 Msa0179100:CDS 40.0%
TCTGTATCTGAGAATGGATG+TGG + chr1_4:66513205-66513224 None:intergenic 40.0%
CATGGTGTTTGTTTGAAAGG+GGG + chr1_4:66513419-66513438 None:intergenic 40.0%
CCATGGTGTTTGTTTGAAAG+GGG + chr1_4:66513420-66513439 None:intergenic 40.0%
GCCATGGTGTTTGTTTGAAA+GGG + chr1_4:66513421-66513440 None:intergenic 40.0%
CGACGGAAGCAAAAATCAAG+AGG + chr1_4:66510707-66510726 None:intergenic 45.0%
TTTCTCAATTGCCGTTGCTC+AGG - chr1_4:66510787-66510806 Msa0179100:three_prime_UTR 45.0%
ATCGTAAACACCGACATAGG+TGG + chr1_4:66510861-66510880 None:intergenic 45.0%
! ACCTATGTCGGTGTTTACGA+TGG - chr1_4:66510860-66510879 Msa0179100:three_prime_UTR 45.0%
ACCATCGTAAACACCGACAT+AGG + chr1_4:66510864-66510883 None:intergenic 45.0%
ACACCAACACCTCTTATCGA+AGG - chr1_4:66511148-66511167 Msa0179100:three_prime_UTR 45.0%
GAATTTGCCTCTGAACAAGG+GGG - chr1_4:66511414-66511433 Msa0179100:CDS 45.0%
!! AAAGGCGTTTAGTAAGACCG+AGG - chr1_4:66511458-66511477 Msa0179100:CDS 45.0%
! CGAGGAGGACTTTTTGCATT+TGG - chr1_4:66511476-66511495 Msa0179100:CDS 45.0%
GACGCAATCTGAGGACTAAT+TGG + chr1_4:66511516-66511535 None:intergenic 45.0%
GTGGGATCATGTTGTCTTCT+TGG - chr1_4:66511537-66511556 Msa0179100:intron 45.0%
!! GGTGACTCTAGAGTTGTTCT+TGG - chr1_4:66511597-66511616 Msa0179100:CDS 45.0%
ATGTAGCTGATGAGGAAGTG+AGG - chr1_4:66511682-66511701 Msa0179100:CDS 45.0%
CAATGTGTGAATCATCAGGG+TGG + chr1_4:66511725-66511744 None:intergenic 45.0%
CCCTGATGATTCACACATTG+TGG - chr1_4:66511725-66511744 Msa0179100:CDS 45.0%
CACATTGTGGTTTATAGCCG+TGG - chr1_4:66511738-66511757 Msa0179100:CDS 45.0%
TGGTTTATAGCCGTGGTGTT+TGG - chr1_4:66511745-66511764 Msa0179100:CDS 45.0%
TTTATAGCCGTGGTGTTTGG+AGG - chr1_4:66511748-66511767 Msa0179100:CDS 45.0%
CACCACAATCACAGACATGT+TGG + chr1_4:66511873-66511892 None:intergenic 45.0%
! TTGTGGTGTAATTTGTCCGG+TGG - chr1_4:66511885-66511904 Msa0179100:intron 45.0%
GAGGCAAGCTTATACAATGC+TGG + chr1_4:66512024-66512043 None:intergenic 45.0%
TTGCCTCGCTTATCAGTTAG+AGG - chr1_4:66512037-66512056 Msa0179100:intron 45.0%
!! TATCAGTTAGAGGTGCTGCA+AGG - chr1_4:66512047-66512066 Msa0179100:intron 45.0%
!! ATCAGTTAGAGGTGCTGCAA+GGG - chr1_4:66512048-66512067 Msa0179100:intron 45.0%
GCTACCCTTACCCCATTTAT+CGG + chr1_4:66512097-66512116 None:intergenic 45.0%
ACTGGACGCTTCAAAGGAAT+AGG + chr1_4:66512229-66512248 None:intergenic 45.0%
GTCATTACTGGACGCTTCAA+AGG + chr1_4:66512235-66512254 None:intergenic 45.0%
GATGGTTCAGCTGTCATTAC+TGG + chr1_4:66512247-66512266 None:intergenic 45.0%
CATAAGACACTCACAGCTCT+TGG + chr1_4:66512379-66512398 None:intergenic 45.0%
! AGCAGCTCTTACTAGTCTCT+TGG + chr1_4:66512488-66512507 None:intergenic 45.0%
ACTAGTAAGAGCTGCTCTTC+AGG - chr1_4:66512492-66512511 Msa0179100:CDS 45.0%
! GAATCTGAGAAGAGCATGCT+TGG - chr1_4:66512712-66512731 Msa0179100:intron 45.0%
! AAGAGCATGCTTGGTTCAGT+TGG - chr1_4:66512721-66512740 Msa0179100:intron 45.0%
TTCGTGAATGTTAGGGTACG+AGG - chr1_4:66513047-66513066 Msa0179100:CDS 45.0%
GAATGTTAGGGTACGAGGTA+AGG - chr1_4:66513052-66513071 Msa0179100:CDS 45.0%
TTGTCTGCAACTTCATGTCG+TGG + chr1_4:66513169-66513188 None:intergenic 45.0%
TCTGAATAGCACGGCAAAAG+TGG + chr1_4:66513315-66513334 None:intergenic 45.0%
! TGCCGTGCTATTCAGATGTT+TGG - chr1_4:66513319-66513338 Msa0179100:five_prime_UTR 45.0%
TCGTTGTCCAACGTGTGTTT+CGG - chr1_4:66513364-66513383 Msa0179100:five_prime_UTR 45.0%
GGGAGAATTGAAGCTAGGAA+AGG + chr1_4:66513399-66513418 None:intergenic 45.0%
AAAGGGGGAGAATTGAAGCT+AGG + chr1_4:66513404-66513423 None:intergenic 45.0%
CCCCTTTCAAACAAACACCA+TGG - chr1_4:66513417-66513436 Msa0179100:five_prime_UTR 45.0%
GGCCATGGTGTTTGTTTGAA+AGG + chr1_4:66513422-66513441 None:intergenic 45.0%
!! ACAAACACCATGGCCTTATG+CGG - chr1_4:66513427-66513446 Msa0179100:five_prime_UTR 45.0%
TCACCTGTGTTTCTTACCAC+AGG - chr1_4:66513471-66513490 Msa0179100:five_prime_UTR 45.0%
! GTTTCAAGTCTGTGTGCCAG+AGG + chr1_4:66510751-66510770 None:intergenic 50.0%
GGCAATTGAGAAATCACCGG+AGG + chr1_4:66510780-66510799 None:intergenic 50.0%
GTAAACACCGACATAGGTGG+CGG + chr1_4:66510858-66510877 None:intergenic 50.0%
! GTCGGTGTTTACGATGGTCA+CGG - chr1_4:66510866-66510885 Msa0179100:three_prime_UTR 50.0%
! ACGCCTTCGATAAGAGGTGT+TGG + chr1_4:66511154-66511173 None:intergenic 50.0%
TGACAATCCCCCTTGTTCAG+AGG + chr1_4:66511424-66511443 None:intergenic 50.0%
TAGTCCTCAGATTGCGTCTG+TGG - chr1_4:66511518-66511537 Msa0179100:intron 50.0%
AGTCCTCAGATTGCGTCTGT+GGG - chr1_4:66511519-66511538 Msa0179100:intron 50.0%
TGTGCTGTATGTTGCCAACC+TGG - chr1_4:66511575-66511594 Msa0179100:intron 50.0%
GTGCTGTATGTTGCCAACCT+GGG - chr1_4:66511576-66511595 Msa0179100:intron 50.0%
!! AACTCTAGAGTCACCCAGGT+TGG + chr1_4:66511592-66511611 None:intergenic 50.0%
!! GAACAACTCTAGAGTCACCC+AGG + chr1_4:66511596-66511615 None:intergenic 50.0%
CCTTGATCCTCCAAACACCA+CGG + chr1_4:66511758-66511777 None:intergenic 50.0%
CGTGGTGTTTGGAGGATCAA+GGG - chr1_4:66511756-66511775 Msa0179100:CDS 50.0%
!! GAGGATCAAGGGCATCATAC+AGG - chr1_4:66511767-66511786 Msa0179100:CDS 50.0%
GCACCTCTAACTGATAAGCG+AGG + chr1_4:66512043-66512062 None:intergenic 50.0%
!! TCAGTTAGAGGTGCTGCAAG+GGG - chr1_4:66512049-66512068 Msa0179100:intron 50.0%
CAGCATGGAAGCTCTTCTAG+TGG - chr1_4:66512622-66512641 Msa0179100:intron 50.0%
TTAGGGTACGAGGTAAGGGT+TGG - chr1_4:66513057-66513076 Msa0179100:CDS 50.0%
! GGACAACGAAAGTACGTGGA+CGG + chr1_4:66513353-66513372 None:intergenic 50.0%
! CGTTGGACAACGAAAGTACG+TGG + chr1_4:66513357-66513376 None:intergenic 50.0%
GCTTTAGCCGAAACACACGT+TGG + chr1_4:66513374-66513393 None:intergenic 50.0%
GTGCCTGTGGTAAGAAACAC+AGG + chr1_4:66513477-66513496 None:intergenic 50.0%
GTGAATGCCGCGTTTAGAGG+GGG + chr1_4:66510537-66510556 None:intergenic 55.0%
GGTGAATGCCGCGTTTAGAG+GGG + chr1_4:66510538-66510557 None:intergenic 55.0%
GGGTGAATGCCGCGTTTAGA+GGG + chr1_4:66510539-66510558 None:intergenic 55.0%
TGGGTGAATGCCGCGTTTAG+AGG + chr1_4:66510540-66510559 None:intergenic 55.0%
! GATTTTTGCTTCCGTCGCCG+TGG - chr1_4:66510710-66510729 Msa0179100:three_prime_UTR 55.0%
TCAAGTCTGTGTGCCAGAGG+AGG + chr1_4:66510748-66510767 None:intergenic 55.0%
GACTTGAAACCTCACGCCTC+CGG - chr1_4:66510761-66510780 Msa0179100:three_prime_UTR 55.0%
TCTCCTTCCGCCACCTATGT+CGG - chr1_4:66510848-66510867 Msa0179100:three_prime_UTR 55.0%
GGTGTTTACGATGGTCACGG+TGG - chr1_4:66510869-66510888 Msa0179100:three_prime_UTR 55.0%
CCAGACACGCCTTCGATAAG+AGG + chr1_4:66511160-66511179 None:intergenic 55.0%
! CCTCTTATCGAAGGCGTGTC+TGG - chr1_4:66511157-66511176 Msa0179100:three_prime_UTR 55.0%
! TCGAAGGCGTGTCTGGTGTT+TGG - chr1_4:66511164-66511183 Msa0179100:three_prime_UTR 55.0%
GGCGTTTAGTAAGACCGAGG+AGG - chr1_4:66511461-66511480 Msa0179100:CDS 55.0%
GATCCCACAGACGCAATCTG+AGG + chr1_4:66511525-66511544 None:intergenic 55.0%
CCGTGGTGTTTGGAGGATCA+AGG - chr1_4:66511755-66511774 Msa0179100:CDS 55.0%
!! CAGTTAGAGGTGCTGCAAGG+GGG - chr1_4:66512050-66512069 Msa0179100:intron 55.0%
TCTTGGCAATTCCCTGCACG+TGG + chr1_4:66512471-66512490 None:intergenic 55.0%
ACAAAAACTGCTGGGTGGGC+AGG - chr1_4:66513097-66513116 Msa0179100:CDS 55.0%
ATCAGGACCGCATAAGGCCA+TGG + chr1_4:66513437-66513456 None:intergenic 55.0%
TTACCACAGGCACAGTCTCG+AGG - chr1_4:66513484-66513503 Msa0179100:five_prime_UTR 55.0%
CCGCGTTTAGAGGGGGAAGA+CGG + chr1_4:66510530-66510549 None:intergenic 60.0%
CCGTCTTCCCCCTCTAAACG+CGG - chr1_4:66510527-66510546 Msa0179100:three_prime_UTR 60.0%
!! GGAGGAAGTCAGAGGCACCA+CGG + chr1_4:66510730-66510749 None:intergenic 60.0%
GTGCCTCTGACTTCCTCCTC+TGG - chr1_4:66510732-66510751 Msa0179100:three_prime_UTR 60.0%
GTGCCAGAGGAGGAAGTCAG+AGG + chr1_4:66510738-66510757 None:intergenic 60.0%
! GAGAAATCACCGGAGGCGTG+AGG + chr1_4:66510773-66510792 None:intergenic 60.0%
!! GACATAGGTGGCGGAAGGAG+AGG + chr1_4:66510849-66510868 None:intergenic 60.0%
!! ACACCGACATAGGTGGCGGA+AGG + chr1_4:66510854-66510873 None:intergenic 60.0%
GGAGGCTGTGCGCTTGTCAA+CGG - chr1_4:66511653-66511672 Msa0179100:CDS 60.0%
!! GAGGTGCTGCAAGGGGGAAA+TGG - chr1_4:66512056-66512075 Msa0179100:intron 60.0%
GCTGCAAGGGGGAAATGGTC+TGG - chr1_4:66512061-66512080 Msa0179100:intron 60.0%
AAAACTGCTGGGTGGGCAGG+TGG - chr1_4:66513100-66513119 Msa0179100:CDS 60.0%
CAACCTCGAGACTGTGCCTG+TGG + chr1_4:66513490-66513509 None:intergenic 60.0%
CACAGGCACAGTCTCGAGGT+TGG - chr1_4:66513488-66513507 Msa0179100:five_prime_UTR 60.0%
!! AGTCAGAGGCACCACGGCGA+CGG + chr1_4:66510724-66510743 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 66510442 66513533 66510442 ID=Msa0179100;Name=Msa0179100
chr1_4 mRNA 66510442 66513533 66510442 ID=Msa0179100-mRNA-1;Parent=Msa0179100;Name=Msa0179100-mRNA-1;_AED=0.03;_eAED=0.03;_QI=241|1|1|1|1|1|4|786|379
chr1_4 exon 66510442 66511497 66510442 ID=Msa0179100-mRNA-1:exon:21815;Parent=Msa0179100-mRNA-1
chr1_4 exon 66511591 66511827 66511591 ID=Msa0179100-mRNA-1:exon:21814;Parent=Msa0179100-mRNA-1
chr1_4 exon 66512187 66512560 66512187 ID=Msa0179100-mRNA-1:exon:21813;Parent=Msa0179100-mRNA-1
chr1_4 exon 66513034 66513533 66513034 ID=Msa0179100-mRNA-1:exon:21812;Parent=Msa0179100-mRNA-1
chr1_4 five_prime_UTR 66513293 66513533 66513293 ID=Msa0179100-mRNA-1:five_prime_utr;Parent=Msa0179100-mRNA-1
chr1_4 CDS 66513034 66513292 66513034 ID=Msa0179100-mRNA-1:cds;Parent=Msa0179100-mRNA-1
chr1_4 CDS 66512187 66512560 66512187 ID=Msa0179100-mRNA-1:cds;Parent=Msa0179100-mRNA-1
chr1_4 CDS 66511591 66511827 66511591 ID=Msa0179100-mRNA-1:cds;Parent=Msa0179100-mRNA-1
chr1_4 CDS 66511228 66511497 66511228 ID=Msa0179100-mRNA-1:cds;Parent=Msa0179100-mRNA-1
chr1_4 three_prime_UTR 66510442 66511227 66510442 ID=Msa0179100-mRNA-1:three_prime_utr;Parent=Msa0179100-mRNA-1
Gene Sequence

>Msa0179100

ATGCTGCGTTTGTGCTTAAGCCCTCTTGATTTTTGCTTCCGTCGCCGTGGTGCCTCTGACTTCCTCCTCTGGCACACAGACTTGAAACCTCACGCCTCCGGTGATTTCTCAATTGCCGTTGCTCAGGCTAATTACTCTCTTGAAGATCAAAGTCAAGTCTTCACCTCTCCTTCCGCCACCTATGTCGGTGTTTACGATGGTCACGGTGGTCCTGAAGCTTCTAGATTTGTCAACAACCGTCTTTTCCCTTATTTACACAAATTTGCCTCTGAACAAGGGGGATTGTCAGCAGATGTGATAAAAAAGGCGTTTAGTAAGACCGAGGAGGACTTTTTGCATTTGGTTAAGTTGTCGTTGCCAATTAGTCCTCAGATTGCGTCTGTGGGATCATGTTGTCTTCTTGGTGCAATTTCTAACAATGTGCTGTATGTTGCCAACCTGGGTGACTCTAGAGTTGTTCTTGGTAGGAAGTATCTTGAGAACAAGAATTCTTCTGTGGAGGCTGTGCGCTTGTCAACGGATCATAATGTAGCTGATGAGGAAGTGAGGAGAGAAGTTGAAGCTCTCCACCCTGATGATTCACACATTGTGGTTTATAGCCGTGGTGTTTGGAGGATCAAGGGCATCATACAGGTGTCTAGATCTATTGGTGATGTATATTTGAAGAGACCAGATTTCTACAGAGATCCCATTTTTCGGCAATTTGGAAATCCTATTCCTTTGAAGCGTCCAGTAATGACAGCTGAACCATCAATCATCATTAGGGAGCTTGAGTCAGATGATTTATTCTTAATATTTGCATCAGATGGCCTTTGGGAACAATTAAGTGATGAAGCAGCAGTTGATATTGTTTTCAAATATCCAAGAGCTGGAATTGCCAAGAGACTAGTAAGAGCTGCTCTTCAGGAAGCTGCAAAGAAAAGAGAGATGAGATATGCTGACATAAAGAAAATTGACAAAGGAATTAGAAGACACTTCCATGACGATATCACTGTTATCGTGATCTATCTTGATCAGCATGGAAGCTCTTCTAGTGGTGAGTTTAAGCAAACTGCTGTTGGCTATACAACTGCCCCTGTTGATATATTTTCTCTAAATGCCGATGAATCTGAGAAGAGCATGCTTGGTTCAGTTGGCTAA
Protein sequence

>Msa0179100

MLRLCLSPLDFCFRRRGASDFLLWHTDLKPHASGDFSIAVAQANYSLEDQSQVFTSPSATYVGVYDGHGGPEASRFVNNRLFPYLHKFASEQGGLSADVIKKAFSKTEEDFLHLVKLSLPISPQIASVGSCCLLGAISNNVLYVANLGDSRVVLGRKYLENKNSSVEAVRLSTDHNVADEEVRREVEALHPDDSHIVVYSRGVWRIKGIIQVSRSIGDVYLKRPDFYRDPIFRQFGNPIPLKRPVMTAEPSIIIRELESDDLFLIFASDGLWEQLSDEAAVDIVFKYPRAGIAKRLVRAALQEAAKKREMRYADIKKIDKGIRRHFHDDITVIVIYLDQHGSSSSGEFKQTAVGYTTAPVDIFSLNADESEKSMLGSVG*