AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0089850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0089850 MtrunA17_Chr1g0207571 99.208 379 3 0 1 379 1 379 0.0 771
Msa0089850 MtrunA17_Chr1g0213351 58.735 332 127 2 17 340 28 357 2.20e-138 399
Msa0089850 MtrunA17_Chr1g0211251 53.743 374 158 3 2 362 9 380 1.62e-137 397
Msa0089850 MtrunA17_Chr7g0250061 51.459 377 161 3 6 362 1 375 8.29e-135 390
Msa0089850 MtrunA17_Chr8g0371321 55.455 330 137 2 17 338 27 354 4.55e-130 378
Msa0089850 MtrunA17_Chr1g0175381 54.969 322 142 1 18 339 36 354 4.88e-129 375
Msa0089850 MtrunA17_Chr2g0294291 53.538 325 148 2 16 339 30 352 1.33e-123 361
Msa0089850 MtrunA17_Chr7g0263281 54.683 331 140 2 16 338 18 346 2.21e-118 348
Msa0089850 MtrunA17_Chr1g0181901 50.140 357 139 4 20 339 27 381 7.00e-112 332
Msa0089850 MtrunA17_Chr3g0089711 46.875 320 159 5 27 339 33 348 2.84e-94 286
Msa0089850 MtrunA17_Chr3g0089721 46.875 320 159 5 27 339 33 348 4.65e-94 286
Msa0089850 MtrunA17_Chr7g0256751 47.040 321 157 6 27 339 34 349 2.53e-86 268
Msa0089850 MtrunA17_Chr7g0256771 47.744 266 132 4 27 289 35 296 6.60e-75 234
Msa0089850 MtrunA17_Chr3g0089691 43.357 286 152 4 81 360 2 283 2.71e-71 225
Msa0089850 MtrunA17_Chr4g0063111 35.326 368 216 9 20 379 34 387 2.11e-62 208
Msa0089850 MtrunA17_Chr4g0063051 33.437 323 175 10 23 339 22 310 5.39e-47 163
Msa0089850 MtrunA17_Chr7g0256761 44.444 180 79 4 163 337 17 180 8.94e-39 137
Msa0089850 MtrunA17_Chr7g0238501 31.208 298 172 10 61 338 263 547 9.35e-37 140
Msa0089850 MtrunA17_Chr2g0295991 34.241 257 149 6 98 344 237 483 1.34e-36 139
Msa0089850 MtrunA17_Chr4g0067301 31.274 259 156 7 98 344 246 494 1.32e-31 125
Msa0089850 MtrunA17_Chr4g0039061 30.137 292 180 7 61 338 202 483 3.15e-31 124
Msa0089850 MtrunA17_Chr1g0193691 29.085 306 154 5 99 346 592 892 1.17e-30 124
Msa0089850 MtrunA17_Chr4g0031521 31.769 277 147 7 98 338 426 696 4.66e-29 119
Msa0089850 MtrunA17_Chr4g0054811 29.412 306 159 6 95 348 478 778 1.58e-28 117
Msa0089850 MtrunA17_Chr3g0105351 29.930 284 135 9 61 340 94 317 4.18e-26 106
Msa0089850 MtrunA17_Chr3g0105371 29.825 285 136 8 60 340 117 341 2.41e-25 105
Msa0089850 MtrunA17_Chr4g0062951 33.161 193 103 7 95 271 1 183 6.73e-25 100
Msa0089850 MtrunA17_Chr1g0212961 29.412 289 133 9 61 346 126 346 1.18e-24 103
Msa0089850 MtrunA17_Chr5g0430481 28.269 283 141 8 61 340 154 377 1.48e-23 100
Msa0089850 MtrunA17_Chr1g0150451 29.617 287 133 9 63 344 110 332 2.56e-21 93.6
Msa0089850 MtrunA17_Chr3g0112291 26.453 344 190 13 29 345 78 385 1.09e-20 92.8
Msa0089850 MtrunA17_Chr4g0070871 30.453 243 113 8 57 286 249 448 1.38e-20 93.2
Msa0089850 MtrunA17_Chr3g0126511 28.163 245 125 7 46 283 106 306 1.56e-20 92.0
Msa0089850 MtrunA17_Chr3g0135961 28.947 304 153 12 54 337 279 539 3.76e-20 92.0
Msa0089850 MtrunA17_Chr3g0136711 29.252 294 140 10 63 352 67 296 4.54e-20 89.7
Msa0089850 MtrunA17_Chr1g0194191 29.316 307 139 11 54 343 112 357 1.43e-19 89.4
Msa0089850 MtrunA17_Chr2g0293811 28.819 288 151 9 54 337 94 331 3.36e-19 88.2
Msa0089850 MtrunA17_Chr5g0407161 27.986 293 135 13 57 339 121 347 1.51e-17 83.2
Msa0089850 MtrunA17_Chr4g0050381 28.322 286 139 10 36 306 32 266 3.29e-17 80.9
Msa0089850 MtrunA17_Chr4g0001761 27.113 284 131 9 46 284 35 287 3.81e-17 82.0
Msa0089850 MtrunA17_Chr3g0136811 26.316 285 132 9 46 284 35 287 6.34e-17 81.3
Msa0089850 MtrunA17_Chr5g0435721 28.017 232 124 6 61 284 223 419 1.09e-16 81.3
Msa0089850 MtrunA17_Chr2g0317021 27.500 280 130 10 64 337 120 332 1.71e-15 76.6
Msa0089850 MtrunA17_Chr1g0150071 27.341 267 126 7 58 284 49 287 2.60e-15 76.6
Msa0089850 MtrunA17_Chr5g0426141 25.974 308 155 11 36 335 37 279 4.54e-15 74.7
Msa0089850 MtrunA17_Chr1g0171571 27.273 297 144 11 52 337 409 644 5.43e-15 76.6
Msa0089850 MtrunA17_Chr1g0158781 28.390 236 127 5 61 294 145 340 7.23e-15 75.5
Msa0089850 MtrunA17_Chr4g0051661 25.952 289 139 11 31 300 149 381 1.22e-14 74.7
Msa0089850 MtrunA17_Chr2g0279151 25.733 307 157 12 36 335 36 278 1.81e-14 73.2
Msa0089850 MtrunA17_Chr3g0144111 27.273 286 117 12 57 335 24 225 3.28e-14 72.0
Msa0089850 MtrunA17_Chr8g0360351 25.965 285 155 11 28 302 29 267 9.09e-14 71.2
Msa0089850 MtrunA17_Chr3g0127091 27.755 245 137 7 60 284 88 312 2.07e-13 70.9
Msa0089850 MtrunA17_Chr3g0115851 26.433 314 146 13 36 335 37 279 5.88e-13 68.6
Msa0089850 MtrunA17_Chr8g0389921 26.222 225 118 6 61 278 198 381 2.36e-12 68.2
Msa0089850 MtrunA17_Chr4g0022171 24.405 336 191 13 54 360 89 390 2.44e-12 68.2
Msa0089850 MtrunA17_Chr6g0483411 24.138 319 165 11 46 340 56 321 8.08e-12 65.9
Msa0089850 MtrunA17_Chr2g0295401 26.073 303 155 13 62 337 93 353 1.11e-11 65.5
Msa0089850 MtrunA17_Chr7g0221691 33.129 163 85 6 142 301 245 386 1.30e-11 65.9
Msa0089850 MtrunA17_Chr7g0272061 25.949 316 150 16 61 337 91 361 4.88e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0089850 AT4G33920.1 70.712 379 109 2 1 378 1 378 0.0 549
Msa0089850 AT4G33920.2 70.169 295 86 2 85 378 2 295 3.01e-147 419
Msa0089850 AT3G51370.1 54.648 355 148 3 1 344 8 360 4.66e-140 404
Msa0089850 AT3G51370.3 54.648 355 148 3 1 344 8 360 4.66e-140 404
Msa0089850 AT5G66080.1 56.196 347 141 2 16 353 28 372 1.10e-139 403
Msa0089850 AT3G12620.2 57.270 337 134 2 17 345 31 365 2.37e-135 392
Msa0089850 AT3G12620.1 57.270 337 134 2 17 345 31 365 2.37e-135 392
Msa0089850 AT4G33920.3 71.053 266 75 2 114 378 1 265 9.38e-134 383
Msa0089850 AT3G55050.1 57.447 329 130 2 17 337 32 358 2.72e-133 387
Msa0089850 AT3G55050.2 57.447 329 130 2 17 337 32 358 2.72e-133 387
Msa0089850 AT4G38520.2 53.955 354 153 2 17 362 29 380 1.31e-131 383
Msa0089850 AT4G38520.1 53.955 354 153 2 17 362 29 380 1.31e-131 383
Msa0089850 AT5G06750.3 57.053 319 136 1 22 339 43 361 3.84e-129 376
Msa0089850 AT5G06750.1 57.053 319 136 1 22 339 43 361 3.84e-129 376
Msa0089850 AT3G17090.1 54.985 331 144 3 22 351 45 371 5.56e-128 373
Msa0089850 AT5G02760.1 53.799 329 140 3 20 340 23 347 3.89e-117 345
Msa0089850 AT3G51370.2 55.797 276 114 3 74 344 3 275 2.65e-109 322
Msa0089850 AT5G66080.2 56.767 266 113 1 88 353 5 268 6.25e-109 321
Msa0089850 AT5G06750.2 57.200 250 106 1 91 339 30 279 3.36e-98 295
Msa0089850 AT3G17090.2 52.963 270 123 2 22 290 45 311 3.75e-98 295
Msa0089850 AT3G16560.1 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0089850 AT3G16560.4 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0089850 AT3G16560.2 33.858 254 154 5 98 345 245 490 2.68e-34 132
Msa0089850 AT5G02400.1 31.884 276 148 6 98 338 396 666 5.92e-33 130
Msa0089850 AT2G46920.3 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0089850 AT2G46920.1 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0089850 AT2G46920.2 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0089850 AT2G46920.4 28.479 309 157 5 99 348 543 846 2.84e-32 129
Msa0089850 AT2G35350.1 28.328 293 157 5 93 337 483 770 9.13e-32 127
Msa0089850 AT2G28890.1 31.317 281 150 6 96 338 371 646 3.61e-31 125
Msa0089850 AT3G09400.1 32.090 268 144 7 100 338 384 642 1.25e-30 124
Msa0089850 AT1G07630.1 31.159 276 150 5 98 338 384 654 1.40e-30 123
Msa0089850 AT2G40180.1 30.142 282 135 9 61 337 161 385 4.23e-28 114
Msa0089850 AT1G67820.2 31.461 267 128 7 60 316 114 335 7.03e-28 113
Msa0089850 AT1G67820.1 31.461 267 128 7 60 316 150 371 9.99e-28 114
Msa0089850 AT3G16560.3 33.641 217 128 4 98 306 245 453 5.57e-26 108
Msa0089850 AT1G07160.1 29.000 300 148 9 46 337 134 376 9.23e-26 107
Msa0089850 AT2G30020.1 30.605 281 133 8 63 339 172 394 4.36e-23 100
Msa0089850 AT3G09400.2 29.851 268 145 10 100 337 384 638 7.20e-22 97.8
Msa0089850 AT4G31750.2 31.048 248 121 8 63 305 66 268 1.62e-21 94.4
Msa0089850 AT4G31750.1 31.048 248 121 8 63 305 66 268 1.63e-21 94.4
Msa0089850 AT4G31750.3 31.048 248 121 8 63 305 66 268 1.63e-21 94.4
Msa0089850 AT1G43900.1 29.893 281 129 10 61 338 154 369 9.56e-21 93.2
Msa0089850 AT1G07430.1 28.520 277 145 9 25 284 114 354 3.60e-20 92.0
Msa0089850 AT2G33700.1 28.767 292 139 11 51 335 113 342 4.73e-19 88.2
Msa0089850 AT2G29380.1 27.124 306 156 12 54 337 100 360 3.48e-18 85.5
Msa0089850 AT5G10740.1 27.536 276 133 8 63 335 66 277 9.10e-18 84.3
Msa0089850 AT5G10740.2 27.536 276 133 8 63 335 66 277 9.10e-18 84.3
Msa0089850 AT1G48040.1 29.730 296 152 10 54 343 110 355 2.35e-17 83.2
Msa0089850 AT3G62260.3 30.315 254 137 10 57 304 24 243 3.16e-17 81.6
Msa0089850 AT5G53140.1 26.531 294 148 10 63 353 134 362 5.73e-17 82.4
Msa0089850 AT5G53140.2 26.531 294 148 10 63 353 134 362 5.73e-17 82.4
Msa0089850 AT2G25620.1 26.408 284 154 11 57 337 125 356 6.23e-17 82.0
Msa0089850 AT2G25620.2 26.408 284 154 11 57 337 125 356 6.23e-17 82.0
Msa0089850 AT4G26080.1 29.153 295 153 13 58 337 169 422 8.14e-17 82.0
Msa0089850 AT3G62260.1 30.315 254 137 10 57 304 113 332 1.01e-16 81.3
Msa0089850 AT5G51760.2 28.099 242 121 9 61 283 114 321 1.07e-16 81.3
Msa0089850 AT3G62260.2 30.315 254 137 10 57 304 114 333 1.20e-16 81.3
Msa0089850 AT5G24940.1 27.536 276 133 8 63 335 66 277 1.34e-16 81.3
Msa0089850 AT5G51760.1 28.099 242 121 9 61 283 144 351 1.38e-16 81.3
Msa0089850 AT5G59220.1 28.333 240 125 8 54 284 141 342 1.53e-16 80.9
Msa0089850 AT1G18030.1 26.990 289 146 10 43 303 83 334 2.15e-15 77.0
Msa0089850 AT4G31860.3 25.818 275 120 11 58 284 49 287 3.55e-15 75.9
Msa0089850 AT4G31860.1 25.818 275 120 11 58 284 49 287 6.78e-15 75.9
Msa0089850 AT3G51470.2 25.168 298 150 10 47 335 48 281 7.40e-15 75.1
Msa0089850 AT3G51470.1 25.168 298 150 10 47 335 92 325 1.12e-14 75.1
Msa0089850 AT1G78200.1 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0089850 AT1G78200.3 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0089850 AT1G78200.2 25.649 308 159 10 36 335 35 280 1.90e-14 73.6
Msa0089850 AT3G16800.2 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0089850 AT3G16800.1 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0089850 AT3G16800.3 27.483 302 150 14 60 337 92 348 2.13e-14 74.3
Msa0089850 AT5G57050.1 25.597 293 167 11 58 337 157 411 2.96e-14 74.3
Msa0089850 AT5G57050.2 25.597 293 167 11 58 337 117 371 3.10e-14 73.9
Msa0089850 AT4G08260.1 26.715 277 116 8 63 339 21 210 4.62e-14 71.2
Msa0089850 AT2G40860.1 28.369 282 137 10 63 337 425 648 9.96e-14 73.2
Msa0089850 AT2G40860.4 28.369 282 137 10 63 337 327 550 9.98e-14 73.2
Msa0089850 AT4G28400.3 27.309 249 129 10 64 306 48 250 1.83e-13 70.5
Msa0089850 AT3G16800.4 28.571 252 124 11 98 337 46 253 1.87e-13 70.1
Msa0089850 AT3G16800.6 28.571 252 124 11 98 337 46 253 1.87e-13 70.1
Msa0089850 AT4G31860.2 26.236 263 110 11 58 272 49 275 2.61e-13 70.1
Msa0089850 AT4G28400.1 27.309 249 129 10 64 306 70 272 2.69e-13 70.1
Msa0089850 AT2G25070.1 26.277 274 118 12 59 284 50 287 3.85e-13 70.5
Msa0089850 AT2G25070.2 26.277 274 118 12 59 284 50 287 3.85e-13 70.5
Msa0089850 AT2G40860.2 27.823 248 136 7 63 303 425 636 4.74e-13 70.9
Msa0089850 AT5G26010.1 26.441 295 174 10 52 337 64 324 5.12e-13 69.7
Msa0089850 AT1G22280.3 26.855 283 153 11 36 306 36 276 9.38e-13 68.6
Msa0089850 AT1G03590.1 26.361 349 186 14 54 371 83 391 2.13e-12 68.6
Msa0089850 AT1G18030.2 27.200 250 130 9 43 273 83 299 2.20e-12 67.8
Msa0089850 AT5G01700.1 26.531 294 169 11 61 339 29 290 2.38e-12 67.8
Msa0089850 AT5G01700.2 26.531 294 169 11 61 339 78 339 2.59e-12 68.2
Msa0089850 AT1G78200.5 26.996 263 138 11 54 306 9 227 3.36e-12 66.2
Msa0089850 AT1G78200.4 26.996 263 138 11 54 306 9 227 3.36e-12 66.2
Msa0089850 AT1G17550.1 25.472 318 158 13 56 337 227 501 8.26e-12 67.0
Msa0089850 AT1G17550.2 25.472 318 158 13 56 337 227 501 8.26e-12 67.0
Msa0089850 AT4G27800.3 27.711 249 136 9 58 284 85 311 1.09e-11 65.9
Msa0089850 AT4G27800.2 27.711 249 136 9 58 284 85 311 1.24e-11 65.9
Msa0089850 AT1G72770.4 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0089850 AT1G72770.1 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0089850 AT1G72770.3 25.472 318 168 10 48 337 225 501 1.39e-11 66.2
Msa0089850 AT2G34740.1 23.642 313 156 10 36 335 90 332 1.60e-11 65.5
Msa0089850 AT4G27800.1 27.106 273 155 9 58 308 85 335 1.95e-11 65.5

Find 141 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATAAGCTGACCTTATTT+TGG 0.149456 1_2:-71615044 Msa0089850:three_prime_UTR
GTGGGATCATGTTGTCTTCT+TGG 0.195703 1_2:-71616276 Msa0089850:CDS
AGATACTGACTGTGTAAATA+AGG 0.264924 1_2:+71616884 None:intergenic
TGCCGTGCTATTCAGATGTT+TGG 0.272533 1_2:-71614494 Msa0089850:three_prime_UTR
TTGGTAAGTTAAGAGAATTC+TGG 0.274260 1_2:-71614989 Msa0089850:three_prime_UTR
ACTAGTAAGAGCTGCTCTTC+AGG 0.291775 1_2:-71615321 Msa0089850:CDS
GATGGTTCAGCTGTCATTAC+TGG 0.293354 1_2:+71615569 None:intergenic
CTGAACCATCAATCATCATT+AGG 0.315695 1_2:-71615556 Msa0089850:CDS
GACGCAATCTGAGGACTAAT+TGG 0.330240 1_2:+71616300 None:intergenic
GGTGACTCTAGAGTTGTTCT+TGG 0.332422 1_2:-71616216 Msa0089850:CDS
TATAGATGATGATGGTGATT+AGG 0.354348 1_2:+71617206 None:intergenic
TATTTGCATCAGATGGCCTT+TGG 0.354565 1_2:-71615505 Msa0089850:CDS
TTAATTGATTAGTCCTTGTA+TGG 0.354606 1_2:-71614803 Msa0089850:three_prime_UTR
TTGACAAATCTAGAAGCTTC+AGG 0.361584 1_2:+71616920 None:intergenic
GCTAATGTTCGTGAATGATA+TGG 0.363428 1_2:-71614773 Msa0089850:three_prime_UTR
TTTAGAGAAAATATATCAAC+AGG 0.367089 1_2:+71615131 None:intergenic
TGAAATGAAATGAAAAGAAA+TGG 0.372964 1_2:+71617244 None:intergenic
AAACAAAAGAGAAGAAGGAA+AGG 0.384883 1_2:-71617332 Msa0089850:five_prime_UTR
TCGTTGTCCAACTTGTGTTT+CGG 0.391265 1_2:-71614449 Msa0089850:three_prime_UTR
ATTTGCATCAGATGGCCTTT+GGG 0.407098 1_2:-71615504 Msa0089850:CDS
ACTGGACGCTTCAAAGGAAT+AGG 0.409207 1_2:+71615587 None:intergenic
TGGGTGAATGCCGCGTTTAG+AGG 0.409840 1_2:+71617276 None:intergenic
GGAAAATGATTAATAGTAAA+GGG 0.415664 1_2:-71617372 Msa0089850:five_prime_UTR
ATGGGATCTCTGTAGAAATC+TGG 0.417500 1_2:+71615629 None:intergenic
ACCACAATGTGTGAATCATC+AGG 0.421762 1_2:+71616087 None:intergenic
GCCGTGGTGTTTGTTTGAAA+GGG 0.421904 1_2:+71614395 None:intergenic
AGCTCCCTAATGATGATTGA+TGG 0.423360 1_2:+71615551 None:intergenic
TGGTTTATAGCCGTGGTGTT+TGG 0.426614 1_2:-71616068 Msa0089850:CDS
GGCCGTGGTGTTTGTTTGAA+AGG 0.429085 1_2:+71614394 None:intergenic
TGAACCATCAATCATCATTA+GGG 0.429934 1_2:-71615555 Msa0089850:CDS
GAATGATATGGTACGAGGTA+AGG 0.432229 1_2:-71614761 Msa0089850:three_prime_UTR
CATAAGATACTCACAGCTCT+TGG 0.439024 1_2:+71615437 None:intergenic
TTCTTAATATTTGCATCAGA+TGG 0.445890 1_2:-71615512 Msa0089850:CDS
AATCGAAGGGTTCGAAATTG+AGG 0.446755 1_2:+71617171 None:intergenic
AGGAAAATGATTAATAGTAA+AGG 0.455208 1_2:-71617373 Msa0089850:five_prime_UTR
TAAGTGAAGATGAATATTGT+TGG 0.457861 1_2:-71615008 Msa0089850:three_prime_UTR
TTTCTCAATTGCCGTTGCTC+AGG 0.469493 1_2:-71617026 Msa0089850:CDS
TTGCAGGTGTCTAGATCTAT+TGG 0.477815 1_2:-71615671 Msa0089850:intron
GATACTGACTGTGTAAATAA+GGG 0.479550 1_2:+71616885 None:intergenic
AAGAGCATGCTTGGTTCAGT+TGG 0.486918 1_2:-71615092 Msa0089850:CDS
GAATCTGAGAAGAGCATGCT+TGG 0.489881 1_2:-71615101 Msa0089850:CDS
AGCAGCTCTTACTAGTCTCT+TGG 0.490227 1_2:+71615328 None:intergenic
AGGGAACATATCTTTGATTG+AGG 0.491568 1_2:-71617312 Msa0089850:five_prime_UTR
TCTGAATAGCACGGCAAAAG+TGG 0.492113 1_2:+71614501 None:intergenic
TGTGTTGTATGTTGCCAACC+TGG 0.492259 1_2:-71616238 Msa0089850:CDS
AACAAAAGAGAAGAAGGAAA+GGG 0.493048 1_2:-71617331 Msa0089850:five_prime_UTR
GACTTGAAACCTCACGCCTC+CGG 0.498145 1_2:-71617052 Msa0089850:CDS
TTTATAGCCGTGGTGTTTGG+AGG 0.501496 1_2:-71616065 Msa0089850:CDS
GGGTGAATGCCGCGTTTAGA+GGG 0.502983 1_2:+71617277 None:intergenic
CCGTGGTGTTTGGAGGATCA+AGG 0.503122 1_2:-71616058 Msa0089850:CDS
GTCATTACTGGACGCTTCAA+AGG 0.503992 1_2:+71615581 None:intergenic
CACATTGTGGTTTATAGCCG+TGG 0.506241 1_2:-71616075 Msa0089850:CDS
GAGGATCAAGGGCATCATAC+AGG 0.507950 1_2:-71616046 Msa0089850:intron
GTGCCTCTGACTTTCTCCTC+TGG 0.508030 1_2:-71617081 Msa0089850:CDS
CCCTGATGATTCACACATTG+TGG 0.513642 1_2:-71616088 Msa0089850:CDS
TAAGAAAACAAAAGAGAAGA+AGG 0.516063 1_2:-71617337 Msa0089850:five_prime_UTR
ACTCTAGAGTTGTTCTTGGT+AGG 0.521401 1_2:-71616212 Msa0089850:CDS
GTAGAATTTGCCTCTGAACA+AGG 0.528771 1_2:-71616402 Msa0089850:intron
TAGTCCTCAGATTGCGTCTG+TGG 0.530528 1_2:-71616295 Msa0089850:CDS
CATGCTCTTCTCAGATTCAT+CGG 0.531284 1_2:+71615106 None:intergenic
ATCAGGACCGCATAAGGCCG+TGG 0.531919 1_2:+71614379 None:intergenic
ATACAGAAAATGTGATAAAG+TGG 0.539215 1_2:-71614595 Msa0089850:three_prime_UTR
TCTCCTTCCGCCACCTATGT+CGG 0.544351 1_2:-71616965 Msa0089850:CDS
TCACCTGTGTTTCTTACCAC+AGG 0.546900 1_2:-71614343 Msa0089850:three_prime_UTR
GGAGGCTGTGCGCTTGTCAA+CGG 0.547101 1_2:-71616160 Msa0089850:CDS
AATGATATGGTACGAGGTAA+GGG 0.547720 1_2:-71614760 Msa0089850:three_prime_UTR
TGATGATGGTGATTAGGTAT+CGG 0.548228 1_2:+71617212 None:intergenic
TAGAATTTGCCTCTGAACAA+GGG 0.551971 1_2:-71616401 Msa0089850:CDS
GCTTTAGCCGAAACACAAGT+TGG 0.552383 1_2:+71614442 None:intergenic
CCGTGGTGTTTGTTTGAAAG+GGG 0.556077 1_2:+71614396 None:intergenic
CGTGGTGTTTGGAGGATCAA+GGG 0.557389 1_2:-71616057 Msa0089850:CDS
AGTCAGAGGCACCACGGCGA+CGG 0.560686 1_2:+71617092 None:intergenic
CGTGGTGTTTGTTTGAAAGG+GGG 0.565718 1_2:+71614397 None:intergenic
TCTGTATGTGAGAATGGATG+TGG 0.566163 1_2:+71614611 None:intergenic
GAGTTTAAGCAAACTGCTGT+TGG 0.576565 1_2:-71615167 Msa0089850:CDS
ACCATCGTAAACACCGACAT+AGG 0.579030 1_2:+71616952 None:intergenic
AAAAGAAATGGAAAAGTCAG+TGG 0.579546 1_2:+71617256 None:intergenic
GACATAGGTGGCGGAAGGAG+AGG 0.580248 1_2:+71616967 None:intergenic
AATGATTAATAGTAAAGGGA+AGG 0.580559 1_2:-71617368 Msa0089850:five_prime_UTR
GAACAAGAATTCTTCTGTGG+AGG 0.580777 1_2:-71616178 Msa0089850:CDS
AACTCTAGAGTCACCCAGGT+TGG 0.586854 1_2:+71616224 None:intergenic
TTGTCTGCAACTTCATGTCG+TGG 0.591077 1_2:+71614647 None:intergenic
CATCACTTAATTGTTCCCAA+AGG 0.592457 1_2:+71615489 None:intergenic
GGAACATATCTTTGATTGAG+GGG 0.592711 1_2:-71617310 Msa0089850:five_prime_UTR
GGATCATAATGTAGCTGATG+AGG 0.594161 1_2:-71616139 Msa0089850:CDS
TGAGAACAAGAATTCTTCTG+TGG 0.594227 1_2:-71616181 Msa0089850:CDS
TATCTATGTATCGAAATCGA+AGG 0.594881 1_2:+71617157 None:intergenic
TGTGTAAATAAGGGAAAAGA+CGG 0.595113 1_2:+71616894 None:intergenic
GTGATCTATCTTGATCAGCA+TGG 0.596846 1_2:-71615206 Msa0089850:CDS
ATCGTAAACACCGACATAGG+TGG 0.597276 1_2:+71616955 None:intergenic
CAGAAAATGTGATAAAGTGG+TGG 0.598245 1_2:-71614592 Msa0089850:three_prime_UTR
ACCTATGTCGGTGTTTACGA+TGG 0.599872 1_2:-71616953 Msa0089850:CDS
TAATAATTTATAGATGATGA+TGG 0.600551 1_2:+71617198 None:intergenic
GGGAGAATTGAAGCTAGGAA+AGG 0.601553 1_2:+71614417 None:intergenic
GGGAACATATCTTTGATTGA+GGG 0.603886 1_2:-71617311 Msa0089850:five_prime_UTR
GTCGGTGTTTACGATGGTCA+CGG 0.604796 1_2:-71616947 Msa0089850:CDS
ACAAACACCACGGCCTTATG+CGG 0.604808 1_2:-71614386 Msa0089850:three_prime_UTR
CGATAACAGTGATATCGTCA+TGG 0.607261 1_2:+71615228 None:intergenic
GGAGAAAGTCAGAGGCACCA+CGG 0.613215 1_2:+71617086 None:intergenic
ATCTATGTATCGAAATCGAA+GGG 0.613366 1_2:+71617158 None:intergenic
GTTTCAAGTCTGTGTGCCAG+AGG 0.616689 1_2:+71617065 None:intergenic
AACGGCAATTGAGAAATCAC+CGG 0.616907 1_2:+71617033 None:intergenic
CCACAATGTGTGAATCATCA+GGG 0.617006 1_2:+71616088 None:intergenic
ATATGGTACGAGGTAAGGGT+TGG 0.619333 1_2:-71614756 Msa0089850:three_prime_UTR
TTACCACAGGCACAGTCTCG+AGG 0.620337 1_2:-71614330 Msa0089850:three_prime_UTR
AGTCCTCAGATTGCGTCTGT+GGG 0.621414 1_2:-71616294 Msa0089850:CDS
GGTGAATGCCGCGTTTAGAG+GGG 0.622833 1_2:+71617278 None:intergenic
AAAGAAATGGAAAAGTCAGT+GGG 0.624711 1_2:+71617257 None:intergenic
CAACCTCGAGACTGTGCCTG+TGG 0.624722 1_2:+71614327 None:intergenic
GGACAACGAAAGTACGTGGA+TGG 0.627942 1_2:+71614463 None:intergenic
GAGAAATCACCGGAGGCGTG+AGG 0.628233 1_2:+71617043 None:intergenic
GTGCCAGAGGAGAAAGTCAG+AGG 0.628468 1_2:+71617078 None:intergenic
AGAATTTGCCTCTGAACAAG+GGG 0.635083 1_2:-71616400 Msa0089850:CDS
GACATAAAGAAAATTGACAA+AGG 0.637095 1_2:-71615266 Msa0089850:CDS
AAAACTGCTTGGTGGGCAGG+TGG 0.639272 1_2:-71614713 Msa0089850:three_prime_UTR
ATGTAGCTGATGAGGAAGTG+AGG 0.643026 1_2:-71616131 Msa0089850:CDS
ACACCGACATAGGTGGCGGA+AGG 0.644257 1_2:+71616962 None:intergenic
ATCCAAACATCTGAATAGCA+CGG 0.645228 1_2:+71614492 None:intergenic
CACAGGCACAGTCTCGAGGT+TGG 0.646521 1_2:-71614326 Msa0089850:three_prime_UTR
GTGTTGTATGTTGCCAACCT+GGG 0.647500 1_2:-71616237 Msa0089850:CDS
GTGCCTGTGGTAAGAAACAC+AGG 0.651716 1_2:+71614340 None:intergenic
AGAGTAATTAGCCTGAGCAA+CGG 0.654865 1_2:+71617015 None:intergenic
GTGAATGCCGCGTTTAGAGG+GGG 0.662612 1_2:+71617279 None:intergenic
GATCCCACAGACGCAATCTG+AGG 0.664939 1_2:+71616291 None:intergenic
GTAAACACCGACATAGGTGG+CGG 0.665590 1_2:+71616958 None:intergenic
CAGCATGGAAGCTCTTCTAG+TGG 0.667037 1_2:-71615191 Msa0089850:CDS
GATGTTTATGATAAGCAGAG+TGG 0.667296 1_2:-71614691 Msa0089850:three_prime_UTR
ATTAGCTATAGATCCATACA+AGG 0.667906 1_2:+71614790 None:intergenic
TAGAGAAAATATATCAACAG+GGG 0.669880 1_2:+71615133 None:intergenic
GAACAACTCTAGAGTCACCC+AGG 0.676212 1_2:+71616220 None:intergenic
AGTTGGACAACGAAAGTACG+TGG 0.681436 1_2:+71614459 None:intergenic
GGCAATTGAGAAATCACCGG+AGG 0.706892 1_2:+71617036 None:intergenic
GGCGTTTAGTAAGACCGAGG+AGG 0.718692 1_2:-71616352 Msa0089850:CDS
TTCGTGAATGATATGGTACG+AGG 0.722011 1_2:-71614766 Msa0089850:three_prime_UTR
TTAGAGAAAATATATCAACA+GGG 0.731235 1_2:+71615132 None:intergenic
CCCCTTTCAAACAAACACCA+CGG 0.736954 1_2:-71614396 Msa0089850:three_prime_UTR
CAATGTGTGAATCATCAGGG+TGG 0.738705 1_2:+71616091 None:intergenic
GAATTTGCCTCTGAACAAGG+GGG 0.739786 1_2:-71616399 Msa0089850:CDS
AAAGGCGTTTAGTAAGACCG+AGG 0.745268 1_2:-71616355 Msa0089850:CDS
CCTTGATCCTCCAAACACCA+CGG 0.748053 1_2:+71616058 None:intergenic
GGTGTTTACGATGGTCACGG+TGG 0.770607 1_2:-71616944 Msa0089850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATTTATAGATGATGA+TGG + chr1_2:71614479-71614498 None:intergenic 15.0%
!! TAAATAATAACTACAAGTAA+TGG + chr1_2:71614821-71614840 None:intergenic 15.0%
!! TATTAAGATCTAATTTGATT+TGG - chr1_2:71614938-71614957 Msa0089850:three_prime_UTR 15.0%
!!! GAATAATTTTATCCAAAATA+AGG + chr1_2:71616645-71616664 None:intergenic 15.0%
!! TGAAATGAAATGAAAAGAAA+TGG + chr1_2:71614433-71614452 None:intergenic 20.0%
!! ACATTAGCAAAAAAAAACAT+AGG + chr1_2:71615211-71615230 None:intergenic 20.0%
!! ACAAATATAATATATACCGT+TGG - chr1_2:71615652-71615671 Msa0089850:CDS 20.0%
!! ACAAAAAATGTACATAATGA+AGG + chr1_2:71615840-71615859 None:intergenic 20.0%
!! TTAGAGAAAATATATCAACA+GGG + chr1_2:71616545-71616564 None:intergenic 20.0%
!! TTTAGAGAAAATATATCAAC+AGG + chr1_2:71616546-71616565 None:intergenic 20.0%
!!! AGTTGTGAAGTTTATATTTT+TGG - chr1_2:71616795-71616814 Msa0089850:intron 20.0%
!!! GTTGTGAAGTTTATATTTTT+GGG - chr1_2:71616796-71616815 Msa0089850:intron 20.0%
! AATGATTAATAGTAAAGGGA+AGG - chr1_2:71614306-71614325 Msa0089850:three_prime_UTR 25.0%
! TAAGAAAACAAAAGAGAAGA+AGG - chr1_2:71614337-71614356 Msa0089850:three_prime_UTR 25.0%
! ATTGACAGAAAATATAACGA+TGG + chr1_2:71615133-71615152 None:intergenic 25.0%
!! TCGTTATATTTTCTGTCAAT+TGG - chr1_2:71615133-71615152 Msa0089850:CDS 25.0%
! AGCAAAAAAAAACATAGGTT+CGG + chr1_2:71615206-71615225 None:intergenic 25.0%
! TAATTGCAAATAAAAACCAC+CGG + chr1_2:71615766-71615785 None:intergenic 25.0%
!!! GTTTATTTTTGCCGATAAAT+GGG - chr1_2:71615944-71615963 Msa0089850:intron 25.0%
!!! TTTATTTTTGCCGATAAATG+GGG - chr1_2:71615945-71615964 Msa0089850:intron 25.0%
! TTCTTAATATTTGCATCAGA+TGG - chr1_2:71616162-71616181 Msa0089850:CDS 25.0%
!! AGAGTTTCAATTTTCTTACA+TGG - chr1_2:71616285-71616304 Msa0089850:CDS 25.0%
! GACATAAAGAAAATTGACAA+AGG - chr1_2:71616408-71616427 Msa0089850:intron 25.0%
! TAGAGAAAATATATCAACAG+GGG + chr1_2:71616544-71616563 None:intergenic 25.0%
! TAAGTGAAGATGAATATTGT+TGG - chr1_2:71616666-71616685 Msa0089850:intron 25.0%
!! TTAATTGATTAGTCCTTGTA+TGG - chr1_2:71616871-71616890 Msa0089850:intron 25.0%
! ATACAGAAAATGTGATAAAG+TGG - chr1_2:71617079-71617098 Msa0089850:CDS 25.0%
! GTGACGATAAAAAAAAAATC+AGG + chr1_2:71617315-71617334 None:intergenic 25.0%
AAACAAAAGAGAAGAAGGAA+AGG - chr1_2:71614342-71614361 Msa0089850:three_prime_UTR 30.0%
AACAAAAGAGAAGAAGGAAA+GGG - chr1_2:71614343-71614362 Msa0089850:three_prime_UTR 30.0%
AAAGAAATGGAAAAGTCAGT+GGG + chr1_2:71614420-71614439 None:intergenic 30.0%
AAAAGAAATGGAAAAGTCAG+TGG + chr1_2:71614421-71614440 None:intergenic 30.0%
!! TATAGATGATGATGGTGATT+AGG + chr1_2:71614471-71614490 None:intergenic 30.0%
ATCTATGTATCGAAATCGAA+GGG + chr1_2:71614519-71614538 None:intergenic 30.0%
TATCTATGTATCGAAATCGA+AGG + chr1_2:71614520-71614539 None:intergenic 30.0%
TGTGTAAATAAGGGAAAAGA+CGG + chr1_2:71614783-71614802 None:intergenic 30.0%
! GATACTGACTGTGTAAATAA+GGG + chr1_2:71614792-71614811 None:intergenic 30.0%
! AGATACTGACTGTGTAAATA+AGG + chr1_2:71614793-71614812 None:intergenic 30.0%
!! ACATGTCAATGTGTTTTTGT+TGG - chr1_2:71615081-71615100 Msa0089850:exon 30.0%
CTTTGTTAATAAGAGACCAA+CGG + chr1_2:71615671-71615690 None:intergenic 30.0%
!!! GGTTTATTTTTGCCGATAAA+TGG - chr1_2:71615943-71615962 Msa0089850:intron 30.0%
!!! CAGTTTTTTGTTTATGTTGC+AGG - chr1_2:71615987-71616006 Msa0089850:intron 30.0%
TTTCCAAATTGCCGAAAAAT+GGG + chr1_2:71616066-71616085 None:intergenic 30.0%
ATTTCCAAATTGCCGAAAAA+TGG + chr1_2:71616067-71616086 None:intergenic 30.0%
TGAACCATCAATCATCATTA+GGG - chr1_2:71616119-71616138 Msa0089850:CDS 30.0%
!! ATCATAAGCTGACCTTATTT+TGG - chr1_2:71616630-71616649 Msa0089850:intron 30.0%
TTGGTAAGTTAAGAGAATTC+TGG - chr1_2:71616685-71616704 Msa0089850:intron 30.0%
! AGTTGCTGACATATTTTTCT+TGG - chr1_2:71616753-71616772 Msa0089850:intron 30.0%
ATTAGCTATAGATCCATACA+AGG + chr1_2:71616887-71616906 None:intergenic 30.0%
! ACATTTTCTGTATGTGAGAA+TGG + chr1_2:71617072-71617091 None:intergenic 30.0%
AGGGAACATATCTTTGATTG+AGG - chr1_2:71614362-71614381 Msa0089850:three_prime_UTR 35.0%
GGGAACATATCTTTGATTGA+GGG - chr1_2:71614363-71614382 Msa0089850:three_prime_UTR 35.0%
GGAACATATCTTTGATTGAG+GGG - chr1_2:71614364-71614383 Msa0089850:three_prime_UTR 35.0%
!! TGATGATGGTGATTAGGTAT+CGG + chr1_2:71614465-71614484 None:intergenic 35.0%
TTGACAAATCTAGAAGCTTC+AGG + chr1_2:71614757-71614776 None:intergenic 35.0%
!! TTATTAGTTTGGTGCAGCAT+TGG - chr1_2:71614870-71614889 Msa0089850:three_prime_UTR 35.0%
CACATTGACATGTCAAACAT+GGG + chr1_2:71615074-71615093 None:intergenic 35.0%
ACACATTGACATGTCAAACA+TGG + chr1_2:71615075-71615094 None:intergenic 35.0%
TAGAATTTGCCTCTGAACAA+GGG - chr1_2:71615273-71615292 Msa0089850:CDS 35.0%
GTCAGCAGATGTGATAAAAA+AGG - chr1_2:71615301-71615320 Msa0089850:CDS 35.0%
TGAGAACAAGAATTCTTCTG+TGG - chr1_2:71615493-71615512 Msa0089850:CDS 35.0%
! GATTGTGGTGTAATTTTGTC+CGG - chr1_2:71615744-71615763 Msa0089850:intron 35.0%
!! TCTACAGAGATCCCATTTTT+CGG - chr1_2:71616052-71616071 Msa0089850:CDS 35.0%
CTGAACCATCAATCATCATT+AGG - chr1_2:71616118-71616137 Msa0089850:CDS 35.0%
CATCACTTAATTGTTCCCAA+AGG + chr1_2:71616188-71616207 None:intergenic 35.0%
GCTAATGTTCGTGAATGATA+TGG - chr1_2:71616901-71616920 Msa0089850:CDS 35.0%
AATGATATGGTACGAGGTAA+GGG - chr1_2:71616914-71616933 Msa0089850:CDS 35.0%
ACCAACTAACAAAAACTGCT+TGG - chr1_2:71616950-71616969 Msa0089850:CDS 35.0%
! ACCAAGCAGTTTTTGTTAGT+TGG + chr1_2:71616954-71616973 None:intergenic 35.0%
AACTAACAAAAACTGCTTGG+TGG - chr1_2:71616953-71616972 Msa0089850:CDS 35.0%
ACTAACAAAAACTGCTTGGT+GGG - chr1_2:71616954-71616973 Msa0089850:CDS 35.0%
GATGTTTATGATAAGCAGAG+TGG - chr1_2:71616983-71617002 Msa0089850:CDS 35.0%
CAGAAAATGTGATAAAGTGG+TGG - chr1_2:71617082-71617101 Msa0089850:CDS 35.0%
ATCCAAACATCTGAATAGCA+CGG + chr1_2:71617185-71617204 None:intergenic 35.0%
AAAAAAATCAGGACCGCATA+AGG + chr1_2:71617304-71617323 None:intergenic 35.0%
AATCGAAGGGTTCGAAATTG+AGG + chr1_2:71614506-71614525 None:intergenic 40.0%
GACGGAAGCAAAAATCAAGA+GGG + chr1_2:71614567-71614586 None:intergenic 40.0%
AACGGCAATTGAGAAATCAC+CGG + chr1_2:71614644-71614663 None:intergenic 40.0%
! AGAGTAATTAGCCTGAGCAA+CGG + chr1_2:71614662-71614681 None:intergenic 40.0%
GTAGAATTTGCCTCTGAACA+AGG - chr1_2:71615272-71615291 Msa0089850:CDS 40.0%
AGAATTTGCCTCTGAACAAG+GGG - chr1_2:71615274-71615293 Msa0089850:CDS 40.0%
CAAATGCAAAAAGTCCTCCT+CGG + chr1_2:71615339-71615358 None:intergenic 40.0%
!! ACTCTAGAGTTGTTCTTGGT+AGG - chr1_2:71615462-71615481 Msa0089850:CDS 40.0%
GAACAAGAATTCTTCTGTGG+AGG - chr1_2:71615496-71615515 Msa0089850:CDS 40.0%
GGATCATAATGTAGCTGATG+AGG - chr1_2:71615535-71615554 Msa0089850:CDS 40.0%
CCACAATGTGTGAATCATCA+GGG + chr1_2:71615589-71615608 None:intergenic 40.0%
ACCACAATGTGTGAATCATC+AGG + chr1_2:71615590-71615609 None:intergenic 40.0%
TACCAACATGTCTGTGATTG+TGG - chr1_2:71615729-71615748 Msa0089850:intron 40.0%
! TTTTGCCGATAAATGGGGTA+AGG - chr1_2:71615950-71615969 Msa0089850:intron 40.0%
TTTGCCGATAAATGGGGTAA+GGG - chr1_2:71615951-71615970 Msa0089850:intron 40.0%
TTGCAGGTGTCTAGATCTAT+TGG - chr1_2:71616003-71616022 Msa0089850:intron 40.0%
ATGGGATCTCTGTAGAAATC+TGG + chr1_2:71616048-71616067 None:intergenic 40.0%
!! GATCCCATTTTTCGGCAATT+TGG - chr1_2:71616060-71616079 Msa0089850:CDS 40.0%
AGCTCCCTAATGATGATTGA+TGG + chr1_2:71616126-71616145 None:intergenic 40.0%
TATTTGCATCAGATGGCCTT+TGG - chr1_2:71616169-71616188 Msa0089850:CDS 40.0%
ATTTGCATCAGATGGCCTTT+GGG - chr1_2:71616170-71616189 Msa0089850:CDS 40.0%
CATAAGATACTCACAGCTCT+TGG + chr1_2:71616240-71616259 None:intergenic 40.0%
!!! TTTGTTTTTGTTCCACGTGC+AGG - chr1_2:71616317-71616336 Msa0089850:CDS 40.0%
!!! TTGTTTTTGTTCCACGTGCA+GGG - chr1_2:71616318-71616337 Msa0089850:CDS 40.0%
CGATAACAGTGATATCGTCA+TGG + chr1_2:71616449-71616468 None:intergenic 40.0%
GTGATCTATCTTGATCAGCA+TGG - chr1_2:71616468-71616487 Msa0089850:intron 40.0%
GAGTTTAAGCAAACTGCTGT+TGG - chr1_2:71616507-71616526 Msa0089850:intron 40.0%
CATGCTCTTCTCAGATTCAT+CGG + chr1_2:71616571-71616590 None:intergenic 40.0%
TTCGTGAATGATATGGTACG+AGG - chr1_2:71616908-71616927 Msa0089850:CDS 40.0%
GAATGATATGGTACGAGGTA+AGG - chr1_2:71616913-71616932 Msa0089850:CDS 40.0%
TCTGTATGTGAGAATGGATG+TGG + chr1_2:71617066-71617085 None:intergenic 40.0%
TCGTTGTCCAACTTGTGTTT+CGG - chr1_2:71617225-71617244 Msa0089850:five_prime_UTR 40.0%
CGACGGAAGCAAAAATCAAG+AGG + chr1_2:71614568-71614587 None:intergenic 45.0%
TTTCTCAATTGCCGTTGCTC+AGG - chr1_2:71614648-71614667 Msa0089850:three_prime_UTR 45.0%
ATCGTAAACACCGACATAGG+TGG + chr1_2:71614722-71614741 None:intergenic 45.0%
! ACCTATGTCGGTGTTTACGA+TGG - chr1_2:71614721-71614740 Msa0089850:three_prime_UTR 45.0%
ACCATCGTAAACACCGACAT+AGG + chr1_2:71614725-71614744 None:intergenic 45.0%
ACACCAACACCTCTTATCGA+AGG - chr1_2:71615011-71615030 Msa0089850:three_prime_UTR 45.0%
GAATTTGCCTCTGAACAAGG+GGG - chr1_2:71615275-71615294 Msa0089850:CDS 45.0%
!! AAAGGCGTTTAGTAAGACCG+AGG - chr1_2:71615319-71615338 Msa0089850:CDS 45.0%
! CGAGGAGGACTTTTTGCATT+TGG - chr1_2:71615337-71615356 Msa0089850:CDS 45.0%
GACGCAATCTGAGGACTAAT+TGG + chr1_2:71615377-71615396 None:intergenic 45.0%
GTGGGATCATGTTGTCTTCT+TGG - chr1_2:71615398-71615417 Msa0089850:intron 45.0%
TGTGTTGTATGTTGCCAACC+TGG - chr1_2:71615436-71615455 Msa0089850:intron 45.0%
GTGTTGTATGTTGCCAACCT+GGG - chr1_2:71615437-71615456 Msa0089850:intron 45.0%
!! GGTGACTCTAGAGTTGTTCT+TGG - chr1_2:71615458-71615477 Msa0089850:CDS 45.0%
ATGTAGCTGATGAGGAAGTG+AGG - chr1_2:71615543-71615562 Msa0089850:CDS 45.0%
CAATGTGTGAATCATCAGGG+TGG + chr1_2:71615586-71615605 None:intergenic 45.0%
CCCTGATGATTCACACATTG+TGG - chr1_2:71615586-71615605 Msa0089850:CDS 45.0%
CACATTGTGGTTTATAGCCG+TGG - chr1_2:71615599-71615618 Msa0089850:CDS 45.0%
TGGTTTATAGCCGTGGTGTT+TGG - chr1_2:71615606-71615625 Msa0089850:CDS 45.0%
TTTATAGCCGTGGTGTTTGG+AGG - chr1_2:71615609-71615628 Msa0089850:CDS 45.0%
CACCACAATCACAGACATGT+TGG + chr1_2:71615734-71615753 None:intergenic 45.0%
! TGTGGTGTAATTTTGTCCGG+TGG - chr1_2:71615747-71615766 Msa0089850:intron 45.0%
GAGGCAAGCTTATACAATGC+TGG + chr1_2:71615885-71615904 None:intergenic 45.0%
TTGCCTCGCTTATCAGTTAG+AGG - chr1_2:71615898-71615917 Msa0089850:intron 45.0%
!! TATCAGTTAGAGGTGCTGCA+AGG - chr1_2:71615908-71615927 Msa0089850:intron 45.0%
!! ATCAGTTAGAGGTGCTGCAA+GGG - chr1_2:71615909-71615928 Msa0089850:intron 45.0%
GCTACCCTTACCCCATTTAT+CGG + chr1_2:71615958-71615977 None:intergenic 45.0%
ACTGGACGCTTCAAAGGAAT+AGG + chr1_2:71616090-71616109 None:intergenic 45.0%
GTCATTACTGGACGCTTCAA+AGG + chr1_2:71616096-71616115 None:intergenic 45.0%
GATGGTTCAGCTGTCATTAC+TGG + chr1_2:71616108-71616127 None:intergenic 45.0%
! AGCAGCTCTTACTAGTCTCT+TGG + chr1_2:71616349-71616368 None:intergenic 45.0%
ACTAGTAAGAGCTGCTCTTC+AGG - chr1_2:71616353-71616372 Msa0089850:CDS 45.0%
! GAATCTGAGAAGAGCATGCT+TGG - chr1_2:71616573-71616592 Msa0089850:intron 45.0%
! AAGAGCATGCTTGGTTCAGT+TGG - chr1_2:71616582-71616601 Msa0089850:intron 45.0%
ATATGGTACGAGGTAAGGGT+TGG - chr1_2:71616918-71616937 Msa0089850:CDS 45.0%
TTGTCTGCAACTTCATGTCG+TGG + chr1_2:71617030-71617049 None:intergenic 45.0%
TCTGAATAGCACGGCAAAAG+TGG + chr1_2:71617176-71617195 None:intergenic 45.0%
! TGCCGTGCTATTCAGATGTT+TGG - chr1_2:71617180-71617199 Msa0089850:five_prime_UTR 45.0%
! AGTTGGACAACGAAAGTACG+TGG + chr1_2:71617218-71617237 None:intergenic 45.0%
GCTTTAGCCGAAACACAAGT+TGG + chr1_2:71617235-71617254 None:intergenic 45.0%
GGGAGAATTGAAGCTAGGAA+AGG + chr1_2:71617260-71617279 None:intergenic 45.0%
AAAGGGGGAGAATTGAAGCT+AGG + chr1_2:71617265-71617284 None:intergenic 45.0%
CGTGGTGTTTGTTTGAAAGG+GGG + chr1_2:71617280-71617299 None:intergenic 45.0%
CCGTGGTGTTTGTTTGAAAG+GGG + chr1_2:71617281-71617300 None:intergenic 45.0%
CCCCTTTCAAACAAACACCA+CGG - chr1_2:71617278-71617297 Msa0089850:five_prime_UTR 45.0%
GCCGTGGTGTTTGTTTGAAA+GGG + chr1_2:71617282-71617301 None:intergenic 45.0%
TCACCTGTGTTTCTTACCAC+AGG - chr1_2:71617331-71617350 Msa0089850:five_prime_UTR 45.0%
!!! TTTTTTTTTTTTTATTAGTT+TGG - chr1_2:71614859-71614878 Msa0089850:three_prime_UTR 5.0%
! GTTTCAAGTCTGTGTGCCAG+AGG + chr1_2:71614612-71614631 None:intergenic 50.0%
GGCAATTGAGAAATCACCGG+AGG + chr1_2:71614641-71614660 None:intergenic 50.0%
GTAAACACCGACATAGGTGG+CGG + chr1_2:71614719-71614738 None:intergenic 50.0%
! GTCGGTGTTTACGATGGTCA+CGG - chr1_2:71614727-71614746 Msa0089850:three_prime_UTR 50.0%
! ACGCCTTCGATAAGAGGTGT+TGG + chr1_2:71615017-71615036 None:intergenic 50.0%
TGACAATCCCCCTTGTTCAG+AGG + chr1_2:71615285-71615304 None:intergenic 50.0%
TAGTCCTCAGATTGCGTCTG+TGG - chr1_2:71615379-71615398 Msa0089850:intron 50.0%
AGTCCTCAGATTGCGTCTGT+GGG - chr1_2:71615380-71615399 Msa0089850:intron 50.0%
!! AACTCTAGAGTCACCCAGGT+TGG + chr1_2:71615453-71615472 None:intergenic 50.0%
!! GAACAACTCTAGAGTCACCC+AGG + chr1_2:71615457-71615476 None:intergenic 50.0%
CCTTGATCCTCCAAACACCA+CGG + chr1_2:71615619-71615638 None:intergenic 50.0%
CGTGGTGTTTGGAGGATCAA+GGG - chr1_2:71615617-71615636 Msa0089850:CDS 50.0%
!! GAGGATCAAGGGCATCATAC+AGG - chr1_2:71615628-71615647 Msa0089850:CDS 50.0%
GCACCTCTAACTGATAAGCG+AGG + chr1_2:71615904-71615923 None:intergenic 50.0%
!! TCAGTTAGAGGTGCTGCAAG+GGG - chr1_2:71615910-71615929 Msa0089850:intron 50.0%
CAGCATGGAAGCTCTTCTAG+TGG - chr1_2:71616483-71616502 Msa0089850:intron 50.0%
! ACAAAAACTGCTTGGTGGGC+AGG - chr1_2:71616958-71616977 Msa0089850:CDS 50.0%
! GGACAACGAAAGTACGTGGA+TGG + chr1_2:71617214-71617233 None:intergenic 50.0%
GGCCGTGGTGTTTGTTTGAA+AGG + chr1_2:71617283-71617302 None:intergenic 50.0%
! ACAAACACCACGGCCTTATG+CGG - chr1_2:71617288-71617307 Msa0089850:five_prime_UTR 50.0%
GTGCCTGTGGTAAGAAACAC+AGG + chr1_2:71617337-71617356 None:intergenic 50.0%
GTGAATGCCGCGTTTAGAGG+GGG + chr1_2:71614398-71614417 None:intergenic 55.0%
GGTGAATGCCGCGTTTAGAG+GGG + chr1_2:71614399-71614418 None:intergenic 55.0%
GGGTGAATGCCGCGTTTAGA+GGG + chr1_2:71614400-71614419 None:intergenic 55.0%
TGGGTGAATGCCGCGTTTAG+AGG + chr1_2:71614401-71614420 None:intergenic 55.0%
! GATTTTTGCTTCCGTCGCCG+TGG - chr1_2:71614571-71614590 Msa0089850:three_prime_UTR 55.0%
!! GGAGAAAGTCAGAGGCACCA+CGG + chr1_2:71614591-71614610 None:intergenic 55.0%
GTGCCTCTGACTTTCTCCTC+TGG - chr1_2:71614593-71614612 Msa0089850:three_prime_UTR 55.0%
GTGCCAGAGGAGAAAGTCAG+AGG + chr1_2:71614599-71614618 None:intergenic 55.0%
GACTTGAAACCTCACGCCTC+CGG - chr1_2:71614622-71614641 Msa0089850:three_prime_UTR 55.0%
TCTCCTTCCGCCACCTATGT+CGG - chr1_2:71614709-71614728 Msa0089850:three_prime_UTR 55.0%
GGTGTTTACGATGGTCACGG+TGG - chr1_2:71614730-71614749 Msa0089850:three_prime_UTR 55.0%
CCAGACACGCCTTCGATAAG+AGG + chr1_2:71615023-71615042 None:intergenic 55.0%
! CCTCTTATCGAAGGCGTGTC+TGG - chr1_2:71615020-71615039 Msa0089850:three_prime_UTR 55.0%
! TCGAAGGCGTGTCTGGTGTT+TGG - chr1_2:71615027-71615046 Msa0089850:three_prime_UTR 55.0%
GGCGTTTAGTAAGACCGAGG+AGG - chr1_2:71615322-71615341 Msa0089850:CDS 55.0%
GATCCCACAGACGCAATCTG+AGG + chr1_2:71615386-71615405 None:intergenic 55.0%
CCGTGGTGTTTGGAGGATCA+AGG - chr1_2:71615616-71615635 Msa0089850:CDS 55.0%
!! CAGTTAGAGGTGCTGCAAGG+GGG - chr1_2:71615911-71615930 Msa0089850:intron 55.0%
TCTTGGCAATTCCCTGCACG+TGG + chr1_2:71616332-71616351 None:intergenic 55.0%
! AAAACTGCTTGGTGGGCAGG+TGG - chr1_2:71616961-71616980 Msa0089850:CDS 55.0%
TTACCACAGGCACAGTCTCG+AGG - chr1_2:71617344-71617363 Msa0089850:five_prime_UTR 55.0%
CCGCGTTTAGAGGGGGAAGA+CGG + chr1_2:71614391-71614410 None:intergenic 60.0%
CCGTCTTCCCCCTCTAAACG+CGG - chr1_2:71614388-71614407 Msa0089850:three_prime_UTR 60.0%
! GAGAAATCACCGGAGGCGTG+AGG + chr1_2:71614634-71614653 None:intergenic 60.0%
!! GACATAGGTGGCGGAAGGAG+AGG + chr1_2:71614710-71614729 None:intergenic 60.0%
!! ACACCGACATAGGTGGCGGA+AGG + chr1_2:71614715-71614734 None:intergenic 60.0%
GGAGGCTGTGCGCTTGTCAA+CGG - chr1_2:71615514-71615533 Msa0089850:CDS 60.0%
!! GAGGTGCTGCAAGGGGGAAA+TGG - chr1_2:71615917-71615936 Msa0089850:intron 60.0%
GCTGCAAGGGGGAAATGGTC+TGG - chr1_2:71615922-71615941 Msa0089850:intron 60.0%
ATCAGGACCGCATAAGGCCG+TGG + chr1_2:71617298-71617317 None:intergenic 60.0%
CAACCTCGAGACTGTGCCTG+TGG + chr1_2:71617350-71617369 None:intergenic 60.0%
CACAGGCACAGTCTCGAGGT+TGG - chr1_2:71617348-71617367 Msa0089850:five_prime_UTR 60.0%
!! AGTCAGAGGCACCACGGCGA+CGG + chr1_2:71614585-71614604 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 71614303 71617393 71614303 ID=Msa0089850;Name=Msa0089850
chr1_2 mRNA 71614303 71617393 71614303 ID=Msa0089850-mRNA-1;Parent=Msa0089850;Name=Msa0089850-mRNA-1;_AED=0.03;_eAED=0.03;_QI=241|1|1|1|1|1|4|785|379
chr1_2 exon 71614303 71615357 71614303 ID=Msa0089850-mRNA-1:exon:24717;Parent=Msa0089850-mRNA-1
chr1_2 exon 71615451 71615687 71615451 ID=Msa0089850-mRNA-1:exon:24716;Parent=Msa0089850-mRNA-1
chr1_2 exon 71616047 71616420 71616047 ID=Msa0089850-mRNA-1:exon:24715;Parent=Msa0089850-mRNA-1
chr1_2 exon 71616894 71617393 71616894 ID=Msa0089850-mRNA-1:exon:24714;Parent=Msa0089850-mRNA-1
chr1_2 five_prime_UTR 71617153 71617393 71617153 ID=Msa0089850-mRNA-1:five_prime_utr;Parent=Msa0089850-mRNA-1
chr1_2 CDS 71616894 71617152 71616894 ID=Msa0089850-mRNA-1:cds;Parent=Msa0089850-mRNA-1
chr1_2 CDS 71616047 71616420 71616047 ID=Msa0089850-mRNA-1:cds;Parent=Msa0089850-mRNA-1
chr1_2 CDS 71615451 71615687 71615451 ID=Msa0089850-mRNA-1:cds;Parent=Msa0089850-mRNA-1
chr1_2 CDS 71615088 71615357 71615088 ID=Msa0089850-mRNA-1:cds;Parent=Msa0089850-mRNA-1
chr1_2 three_prime_UTR 71614303 71615087 71614303 ID=Msa0089850-mRNA-1:three_prime_utr;Parent=Msa0089850-mRNA-1
Gene Sequence

>Msa0089850

ATGCTGCGTTTGTGCTTAAGCCCTCTTGATTTTTGCTTCCGTCGCCGTGGTGCCTCTGACTTTCTCCTCTGGCACACAGACTTGAAACCTCACGCCTCCGGTGATTTCTCAATTGCCGTTGCTCAGGCTAATTACTCTCTTGAAGATCAAAGTCAAGTCTTCACCTCTCCTTCCGCCACCTATGTCGGTGTTTACGATGGTCACGGTGGTCCTGAAGCTTCTAGATTTGTCAACAACCGTCTTTTCCCTTATTTACACAAATTTGCCTCTGAACAAGGGGGATTGTCAGCAGATGTGATAAAAAAGGCGTTTAGTAAGACCGAGGAGGACTTTTTGCATTTGGTTAAGTTGTCGTTGCCAATTAGTCCTCAGATTGCGTCTGTGGGATCATGTTGTCTTCTTGGTGCAATTTCTAACAATGTGTTGTATGTTGCCAACCTGGGTGACTCTAGAGTTGTTCTTGGTAGGAAGTATCTTGAGAACAAGAATTCTTCTGTGGAGGCTGTGCGCTTGTCAACGGATCATAATGTAGCTGATGAGGAAGTGAGGAGAGAAGTTGAAGCTCTCCACCCTGATGATTCACACATTGTGGTTTATAGCCGTGGTGTTTGGAGGATCAAGGGCATCATACAGGTGTCTAGATCTATTGGTGATGTATATTTGAAGAGACCAGATTTCTACAGAGATCCCATTTTTCGGCAATTTGGAAATCCTATTCCTTTGAAGCGTCCAGTAATGACAGCTGAACCATCAATCATCATTAGGGAGCTTGAGTCAGATGATTTATTCTTAATATTTGCATCAGATGGCCTTTGGGAACAATTAAGTGATGAAGCAGCAGTTGATATTGTTTTCAAATATCCAAGAGCTGGAATTGCCAAGAGACTAGTAAGAGCTGCTCTTCAGGAAGCTGCAAAGAAAAGAGAGATGAGATATGCTGACATAAAGAAAATTGACAAAGGAATTAGAAGACACTTCCATGACGATATCACTGTTATCGTGATCTATCTTGATCAGCATGGAAGCTCTTCTAGTGGTGAGTTTAAGCAAACTGCTGTTGGCTATACAACTGCCCCTGTTGATATATTTTCTCTAAATGCCGATGAATCTGAGAAGAGCATGCTTGGTTCAGTTGGCTAA
Protein sequence

>Msa0089850

MLRLCLSPLDFCFRRRGASDFLLWHTDLKPHASGDFSIAVAQANYSLEDQSQVFTSPSATYVGVYDGHGGPEASRFVNNRLFPYLHKFASEQGGLSADVIKKAFSKTEEDFLHLVKLSLPISPQIASVGSCCLLGAISNNVLYVANLGDSRVVLGRKYLENKNSSVEAVRLSTDHNVADEEVRREVEALHPDDSHIVVYSRGVWRIKGIIQVSRSIGDVYLKRPDFYRDPIFRQFGNPIPLKRPVMTAEPSIIIRELESDDLFLIFASDGLWEQLSDEAAVDIVFKYPRAGIAKRLVRAALQEAAKKREMRYADIKKIDKGIRRHFHDDITVIVIYLDQHGSSSSGEFKQTAVGYTTAPVDIFSLNADESEKSMLGSVG*