AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0141730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0141730 MtrunA17_Chr1g0165671 97.284 405 11 0 1 405 73 477 0.0 815
Msa0141730 MtrunA17_Chr1g0165681 75.676 407 97 2 1 405 55 461 0.0 642
Msa0141730 MtrunA17_Chr1g0165661 74.693 407 98 3 1 405 55 458 0.0 615
Msa0141730 MtrunA17_Chr1g0165711 72.906 406 66 4 1 405 55 417 0.0 584
Msa0141730 MtrunA17_Chr5g0433641 58.537 410 160 6 1 405 56 460 5.24e-174 493
Msa0141730 MtrunA17_Chr7g0273791 58.603 401 157 6 1 396 56 452 4.64e-172 489
Msa0141730 MtrunA17_Chr7g0273781 57.750 400 154 7 1 392 89 481 1.49e-169 483
Msa0141730 MtrunA17_Chr7g0273721 57.805 410 164 5 1 405 57 462 2.30e-169 482
Msa0141730 MtrunA17_Chr1g0191081 60.982 387 146 5 1 384 64 448 1.26e-165 472
Msa0141730 MtrunA17_Chr7g0273761 57.385 413 161 8 1 405 428 833 6.59e-165 483
Msa0141730 MtrunA17_Chr7g0273761 55.449 312 127 6 92 396 32 338 1.97e-109 340
Msa0141730 MtrunA17_Chr7g0273731 57.841 389 156 5 1 384 139 524 9.15e-164 470
Msa0141730 MtrunA17_Chr6g0471381 69.811 265 78 2 143 405 6 270 5.09e-134 385
Msa0141730 MtrunA17_Chr6g0471971 51.477 237 86 6 107 337 1 214 9.41e-78 239
Msa0141730 MtrunA17_Chr3g0132001 34.474 380 235 7 2 376 58 428 1.22e-75 241
Msa0141730 MtrunA17_Chr8g0386141 33.588 393 248 6 3 390 52 436 4.15e-74 238
Msa0141730 MtrunA17_Chr6g0471891 60.406 197 52 3 176 370 1 173 2.80e-72 226
Msa0141730 MtrunA17_Chr7g0224391 35.588 340 210 5 2 335 60 396 2.27e-71 230
Msa0141730 MtrunA17_Chr7g0273701 57.303 178 70 4 230 405 1 174 7.14e-64 202
Msa0141730 MtrunA17_Chr2g0306321 31.807 393 247 12 8 384 76 463 9.45e-54 185
Msa0141730 MtrunA17_Chr1g0165721 75.214 117 29 0 289 405 1 117 2.75e-53 173
Msa0141730 MtrunA17_Chr4g0027071 29.442 394 259 7 4 381 63 453 1.04e-52 182
Msa0141730 MtrunA17_Chr1g0165731 70.370 108 31 1 107 213 1 108 4.56e-51 167
Msa0141730 MtrunA17_Chr4g0001861 32.031 384 233 13 9 376 55 426 4.90e-51 177
Msa0141730 MtrunA17_Chr1g0165741 86.022 93 13 0 1 93 55 147 5.26e-51 168
Msa0141730 MtrunA17_Chr3g0141851 33.429 347 213 9 7 340 58 399 1.40e-49 174
Msa0141730 MtrunA17_Chr6g0471371 81.250 96 18 0 1 96 55 150 8.65e-49 163
Msa0141730 MtrunA17_Chr2g0334001 30.184 381 243 9 9 376 55 425 4.59e-48 169
Msa0141730 MtrunA17_Chr8g0371821 30.909 385 239 10 9 380 55 425 5.99e-47 166
Msa0141730 MtrunA17_Chr8g0353421 96.000 75 3 0 1 75 55 129 4.89e-44 149
Msa0141730 MtrunA17_Chr3g0113821 30.311 386 241 11 10 380 60 432 4.41e-42 153
Msa0141730 MtrunA17_Chr6g0470591 29.340 409 253 13 2 383 63 462 5.42e-42 153
Msa0141730 MtrunA17_Chr2g0283361 30.052 386 256 11 5 380 53 434 1.89e-41 152
Msa0141730 MtrunA17_Chr7g0253501 28.605 423 228 16 7 378 52 451 2.05e-37 141
Msa0141730 MtrunA17_Chr4g0072041 27.318 399 250 11 10 380 70 456 4.14e-37 140
Msa0141730 MtrunA17_Chr7g0273741 60.215 93 37 0 1 93 56 148 7.38e-37 131
Msa0141730 MtrunA17_Chr7g0253541 27.318 399 239 16 7 368 57 441 1.78e-36 138
Msa0141730 MtrunA17_Chr7g0253531 27.578 417 240 15 7 378 52 451 9.96e-36 136
Msa0141730 MtrunA17_Chr3g0098961 61.290 93 36 0 1 93 59 151 3.25e-34 125
Msa0141730 MtrunA17_Chr7g0252731 29.326 341 199 11 10 335 70 383 1.12e-33 130
Msa0141730 MtrunA17_Chr4g0054081 27.820 399 249 13 10 380 65 452 6.39e-33 128
Msa0141730 MtrunA17_Chr3g0087401 25.069 363 235 8 7 336 51 409 3.27e-32 126
Msa0141730 MtrunA17_Chr7g0270771 30.738 244 137 6 3 235 48 270 7.54e-32 122
Msa0141730 MtrunA17_Chr7g0235531 27.110 391 242 12 13 378 61 433 9.01e-32 125
Msa0141730 MtrunA17_Chr3g0106691 28.117 409 251 13 5 383 109 504 3.99e-31 124
Msa0141730 MtrunA17_Chr3g0106661 29.398 415 250 14 2 383 60 464 1.20e-29 119
Msa0141730 MtrunA17_Chr2g0311401 27.815 302 197 8 88 376 2 295 1.51e-29 116
Msa0141730 MtrunA17_Chr3g0106761 26.716 408 257 14 5 383 9 403 2.68e-28 115
Msa0141730 MtrunA17_Chr3g0106741 27.763 389 239 13 24 383 59 434 5.99e-28 114
Msa0141730 MtrunA17_Chr3g0106721 28.523 298 188 7 2 282 60 349 1.71e-27 113
Msa0141730 MtrunA17_Chr7g0273691 54.945 91 37 2 111 198 1 90 2.30e-27 104
Msa0141730 MtrunA17_Chr4g0053081 25.570 395 253 14 3 374 57 433 4.65e-27 112
Msa0141730 MtrunA17_Chr5g0442701 26.065 399 235 14 7 371 63 435 1.81e-25 107
Msa0141730 MtrunA17_Chr4g0053111 27.204 397 244 16 5 374 57 435 2.46e-25 107
Msa0141730 MtrunA17_Chr3g0134071 28.804 368 217 16 10 345 70 424 2.78e-25 107
Msa0141730 MtrunA17_Chr5g0442691 25.490 408 226 14 7 371 86 458 4.86e-25 106
Msa0141730 MtrunA17_Chr5g0442721 26.250 400 234 15 7 371 64 437 9.82e-25 105
Msa0141730 MtrunA17_Chr4g0010121 27.072 362 224 13 5 341 94 440 2.39e-24 104
Msa0141730 MtrunA17_Chr3g0134091 30.000 280 176 10 7 273 65 337 2.82e-24 104
Msa0141730 MtrunA17_Chr8g0374911 25.307 407 244 16 5 374 75 458 9.79e-24 102
Msa0141730 MtrunA17_Chr4g0053061 25.061 407 245 16 5 374 82 465 2.35e-23 101
Msa0141730 MtrunA17_Chr4g0010361 27.250 400 241 19 5 374 50 429 2.43e-23 101
Msa0141730 MtrunA17_Chr8g0359251 27.182 401 232 18 5 374 58 429 3.36e-23 100
Msa0141730 MtrunA17_Chr7g0224131 28.316 392 243 18 5 374 50 425 4.33e-23 100
Msa0141730 MtrunA17_Chr3g0134041 26.984 378 213 15 7 345 63 416 4.95e-23 100
Msa0141730 MtrunA17_Chr3g0095721 25.000 404 220 14 10 369 72 436 9.19e-23 99.8
Msa0141730 MtrunA17_Chr8g0374641 27.160 405 234 20 5 374 54 432 1.67e-22 99.0
Msa0141730 MtrunA17_Chr3g0106711 37.762 143 83 3 5 143 64 204 2.10e-22 95.1
Msa0141730 MtrunA17_Chr3g0125001 27.848 395 250 16 5 381 50 427 2.15e-22 98.6
Msa0141730 MtrunA17_Chr4g0053091 24.242 396 245 12 2 374 59 422 3.16e-22 98.2
Msa0141730 MtrunA17_Chr3g0134061 25.135 370 240 13 7 345 65 428 5.38e-22 97.4
Msa0141730 MtrunA17_Chr4g0010331 26.031 388 254 14 5 374 50 422 1.33e-21 96.3
Msa0141730 MtrunA17_Chr8g0354371 25.700 393 246 15 5 374 59 428 1.87e-21 95.9
Msa0141730 MtrunA17_Chr3g0134121 26.471 408 249 18 7 387 66 449 3.30e-21 95.5
Msa0141730 MtrunA17_Chr4g0010311 26.221 389 248 17 10 374 55 428 4.16e-21 94.7
Msa0141730 MtrunA17_Chr3g0123061 24.485 388 242 15 8 381 59 409 2.05e-20 92.4
Msa0141730 MtrunA17_Chr3g0134111 27.151 372 224 15 7 345 62 419 4.59e-20 91.7
Msa0141730 MtrunA17_Chr4g0010351 30.037 273 173 9 10 267 55 324 1.17e-18 87.0
Msa0141730 MtrunA17_Chr5g0438701 22.874 341 223 12 10 332 59 377 2.06e-18 86.7
Msa0141730 MtrunA17_Chr5g0438681 23.684 342 218 12 10 332 59 376 3.70e-18 85.9
Msa0141730 MtrunA17_Chr7g0273681 65.306 49 17 0 37 85 1 49 1.27e-17 76.3
Msa0141730 MtrunA17_Chr7g0252771 27.711 249 164 6 137 376 6 247 7.88e-17 79.7
Msa0141730 MtrunA17_Chr1g0199561 23.014 365 237 12 10 347 60 407 1.11e-16 81.6
Msa0141730 MtrunA17_Chr6g0471981 57.812 64 25 1 46 109 1 62 7.32e-16 71.6
Msa0141730 MtrunA17_Chr8g0376891 22.034 413 267 13 7 394 56 438 2.07e-15 77.8
Msa0141730 MtrunA17_Chr5g0414431 63.462 52 14 1 1 52 6 52 1.40e-14 67.8
Msa0141730 MtrunA17_Chr4g0004691 27.966 118 85 0 10 127 53 170 2.25e-13 68.2
Msa0141730 MtrunA17_Chr5g0438721 32.000 125 68 5 10 128 9 122 1.60e-12 64.7
Msa0141730 MtrunA17_Chr4g0004701 27.273 198 116 8 199 376 45 234 2.40e-12 66.6
Msa0141730 MtrunA17_Chr1g0169471 35.052 97 55 2 33 128 11 100 3.43e-12 63.2
Msa0141730 MtrunA17_Chr3g0097841 26.923 156 96 3 195 334 47 200 1.74e-11 63.9
Msa0141730 MtrunA17_Chr2g0311411 40.000 90 52 1 9 98 55 142 2.56e-11 61.6
Msa0141730 MtrunA17_Chr3g0134131 28.395 162 112 1 174 331 122 283 3.39e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0141730 AT5G17540.1 58.333 408 159 8 5 405 58 461 1.35e-161 462
Msa0141730 AT3G03480.1 57.216 388 154 9 5 385 68 450 2.46e-148 428
Msa0141730 AT5G41040.2 34.021 388 237 8 2 380 61 438 7.01e-78 248
Msa0141730 AT5G41040.1 34.021 388 237 8 2 380 77 454 9.39e-78 248
Msa0141730 AT5G63560.1 33.592 387 236 9 2 381 53 425 2.87e-71 230
Msa0141730 AT3G48720.1 31.937 382 243 9 2 376 53 424 3.30e-67 219
Msa0141730 AT3G62160.1 31.111 405 238 15 3 390 48 428 3.71e-52 180
Msa0141730 AT1G27620.1 31.043 393 246 10 10 390 58 437 1.22e-51 179
Msa0141730 AT1G65450.1 31.122 392 239 10 10 384 72 449 3.93e-48 170
Msa0141730 AT1G28680.1 29.825 399 260 12 1 385 53 445 7.47e-47 167
Msa0141730 AT2G19070.1 29.208 404 245 13 7 380 55 447 7.51e-46 164
Msa0141730 AT5G57840.1 28.718 390 240 8 12 378 58 432 1.39e-44 160
Msa0141730 AT2G40230.1 33.036 336 210 8 10 334 59 390 1.47e-44 160
Msa0141730 AT5G48930.1 29.231 390 244 10 9 380 55 430 1.71e-44 160
Msa0141730 AT1G03390.1 29.948 384 252 12 10 380 76 455 3.14e-43 157
Msa0141730 AT1G28680.2 32.781 302 192 8 1 295 53 350 1.60e-42 154
Msa0141730 AT3G47170.1 27.635 427 235 16 6 384 66 466 4.21e-39 146
Msa0141730 AT5G07080.1 28.710 411 230 15 5 381 65 446 7.14e-38 142
Msa0141730 AT2G25150.1 29.104 402 255 14 5 385 62 454 4.51e-36 137
Msa0141730 AT2G23510.1 29.136 405 241 15 6 383 65 450 2.01e-35 135
Msa0141730 AT1G78990.1 27.820 399 250 12 8 385 65 446 8.85e-33 128
Msa0141730 AT5G07860.1 27.638 398 234 14 10 381 72 441 8.12e-28 114
Msa0141730 AT1G65450.3 28.041 296 184 8 105 384 3 285 2.25e-27 110
Msa0141730 AT5G07870.1 27.654 405 225 16 10 374 72 448 2.77e-26 110
Msa0141730 AT1G31490.1 25.788 349 216 11 7 333 59 386 3.18e-26 110
Msa0141730 AT1G32910.1 25.990 404 258 13 8 385 63 451 5.13e-26 109
Msa0141730 AT5G42830.1 28.989 376 211 14 7 345 61 417 9.11e-25 105
Msa0141730 AT5G07850.1 27.019 359 232 13 10 345 70 421 1.75e-23 102
Msa0141730 AT3G50270.1 27.419 372 211 14 10 343 62 412 2.82e-23 101
Msa0141730 AT3G50280.1 26.611 357 231 15 10 343 59 407 3.69e-23 101
Msa0141730 AT4G31910.1 26.904 394 255 11 10 376 64 451 5.86e-23 100
Msa0141730 AT5G67150.1 25.628 398 242 16 10 376 61 435 8.84e-23 100
Msa0141730 AT1G24430.1 24.304 395 247 15 1 376 53 414 7.08e-22 97.4
Msa0141730 AT3G50300.1 26.685 356 222 13 10 338 59 402 8.45e-22 97.4
Msa0141730 AT5G38130.1 25.706 354 230 12 13 343 82 425 5.17e-21 95.1
Msa0141730 AT5G16410.1 25.862 348 223 12 8 334 89 422 9.28e-20 91.3
Msa0141730 AT4G15390.1 24.304 395 241 15 10 376 64 428 1.82e-19 90.5
Msa0141730 AT4G15390.2 24.304 395 241 15 10 376 64 428 1.82e-19 90.5
Msa0141730 AT5G67160.1 26.406 409 238 15 4 381 50 426 3.00e-19 89.7
Msa0141730 AT1G24420.2 25.816 337 195 14 20 332 75 380 3.36e-19 89.4
Msa0141730 AT1G24420.1 25.816 337 195 14 20 332 75 380 3.36e-19 89.4
Msa0141730 AT3G50280.2 26.007 273 184 10 84 343 10 277 6.72e-16 78.6
Msa0141730 AT5G23940.1 24.823 423 238 16 7 384 62 449 9.57e-16 79.3
Msa0141730 AT3G50290.1 26.022 269 160 9 84 328 16 269 7.17e-14 72.0
Msa0141730 AT3G23840.1 28.643 199 117 7 26 215 81 263 4.58e-12 67.8
Msa0141730 AT4G15400.1 25.060 415 244 19 10 394 58 435 1.16e-11 66.6
Msa0141730 AT5G47950.1 23.684 380 238 16 10 373 62 405 3.09e-11 65.1

Find 134 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCCATTGTTGGTGATTC+AGG 0.100165 1_4:+436886 Msa0141730:CDS
TTCAAACCAACATTAGTTTA+AGG 0.181902 1_4:-439511 None:intergenic
AATTATACCTTTCCTCATTA+TGG 0.183641 1_4:+436450 Msa0141730:five_prime_UTR
TGCGGTGAGGATTTCGAAAT+TGG 0.188633 1_4:-438899 None:intergenic
GGGTCTTTCCCTGCCATATT+TGG 0.199762 1_4:-436626 None:intergenic
GCATTCAGTTGCGGATTTAA+TGG 0.222413 1_4:+439158 Msa0141730:CDS
TTTGAGGGTCTTACAAATTC+AGG 0.246276 1_4:-436508 None:intergenic
TTTGAAAACGTAACCCTTCT+TGG 0.271683 1_4:-436574 None:intergenic
GGTATTTAGCTCAATTAATA+AGG 0.282791 1_4:-439614 None:intergenic
TTGATAGTGGTTATTATAGA+AGG 0.302952 1_4:-436409 None:intergenic
TAAACGAACTTATGATAATA+AGG 0.304041 1_4:-439814 None:intergenic
CACCTAAGGCACTCATGAAT+TGG 0.305227 1_4:-438645 None:intergenic
TGAATTTCCTTTGCTATGAT+TGG 0.316077 1_4:+439530 Msa0141730:three_prime_UTR
GCGATGGGCCATGTCATCTA+AGG 0.318314 1_4:-438806 None:intergenic
ATCCATTTGCAGGTAGGTTA+AGG 0.320236 1_4:+436696 Msa0141730:CDS
TAAGGGAATAATTGTTCCTT+TGG 0.330029 1_4:-438788 None:intergenic
TTGGAGATGTTGACTTTGGT+TGG 0.341202 1_4:+439250 Msa0141730:CDS
GGTTGGGGAAAAGCTGTATA+TGG 0.341822 1_4:+439267 Msa0141730:CDS
CCCGTCGCGGTTACAACCGC+TGG 0.344616 1_4:+439057 Msa0141730:CDS
GATGTTAGTTTGAAAGAATT+TGG 0.347283 1_4:+436788 Msa0141730:CDS
GAAACAATGAAACTAGGTTT+TGG 0.349312 1_4:-439477 None:intergenic
TTATAAGTATGATCCAAATA+TGG 0.357454 1_4:+436613 Msa0141730:exon
GATGTGACACGTGCTGGATT+TGG 0.363433 1_4:+439231 Msa0141730:CDS
TGCTAAAGGAGAGGAAGGTT+TGG 0.383515 1_4:+439356 Msa0141730:CDS
TGGAGATGTTGACTTTGGTT+GGG 0.387463 1_4:+439251 Msa0141730:CDS
CTGCTGCGTCACACATTGTA+TGG 0.389180 1_4:-438615 None:intergenic
AACATTAACGAACCTACTAT+TGG 0.395610 1_4:+439450 Msa0141730:CDS
AAATCCTCACCGCATGCCTT+TGG 0.410833 1_4:+438908 Msa0141730:CDS
ATAATTGTTCCTTTGGTGTC+GGG 0.411133 1_4:-438781 None:intergenic
CGAACTTCTTCGTCAGTGTC+TGG 0.418327 1_4:-438955 None:intergenic
AAACTAATGGAAAATCCGTT+AGG 0.419834 1_4:+439081 Msa0141730:CDS
TCCATTTGCAGGTAGGTTAA+GGG 0.425790 1_4:+436697 Msa0141730:CDS
TGCGGATTTAATGGTTATCA+AGG 0.430701 1_4:+439167 Msa0141730:CDS
CCTTAGGTGAAATTTCTTGC+GGG 0.433387 1_4:+438659 Msa0141730:CDS
GTCCAATTCATGAGTGCCTT+AGG 0.433988 1_4:+438643 Msa0141730:CDS
TACAACCGCTGGGAAACTAA+TGG 0.437340 1_4:+439068 Msa0141730:CDS
TCCCTTAACCTACCTGCAAA+TGG 0.437418 1_4:-436698 None:intergenic
CCAGCGGTTGTAACCGCGAC+GGG 0.438069 1_4:-439057 None:intergenic
AATGTGTGACGCAGCAGGTT+TGG 0.438562 1_4:+438621 Msa0141730:CDS
TCACTGTGAATGATAGAGAT+TGG 0.444139 1_4:-436474 None:intergenic
AGGTCCTGACAGGAAACTTA+TGG 0.445457 1_4:+436721 Msa0141730:CDS
AGTGCTTAACTCTCCATTGT+TGG 0.450887 1_4:+436877 Msa0141730:CDS
GAAAGGTATAATTGAAGAAA+GGG 0.453489 1_4:-436440 None:intergenic
GCATTACCGTAGTAACCATT+TGG 0.454482 1_4:-439024 None:intergenic
ATATAATGCGCCACATGCAT+TGG 0.460594 1_4:-436366 None:intergenic
TACTTATAAAATTGTATCAC+TGG 0.463814 1_4:-436599 None:intergenic
AAGGGTACTAAATTTGATAG+TGG 0.464191 1_4:-436422 None:intergenic
CACTGTGAATGATAGAGATT+GGG 0.466767 1_4:-436473 None:intergenic
TTGAGGGTCTTACAAATTCA+GGG 0.476311 1_4:-436507 None:intergenic
GGAAAGGTATAATTGAAGAA+AGG 0.476453 1_4:-436441 None:intergenic
CAATCTCACCGGTATGGCAT+AGG 0.484304 1_4:+438695 Msa0141730:CDS
TGTAGACCTTAAACTAATGT+TGG 0.490789 1_4:+439505 Msa0141730:exon
TATAATGCGCCACATGCATT+GGG 0.492129 1_4:-436365 None:intergenic
TTTAATCCACCGTTACCAAA+TGG 0.496653 1_4:+439009 Msa0141730:CDS
GGATTTGGAGATGTTGACTT+TGG 0.497797 1_4:+439246 Msa0141730:CDS
AAGTATGATCCAAATATGGC+AGG 0.501582 1_4:+436617 Msa0141730:exon
GTGTCTGATGTGACACGTGC+TGG 0.502256 1_4:+439225 Msa0141730:CDS
TGAGTACATGTTACTCTTGG+TGG 0.504345 1_4:-438739 None:intergenic
CGAATTGTGGCTACCTCGGT+TGG 0.505273 1_4:-438844 None:intergenic
AGCTCATCCAAACAAGGAAA+AGG 0.507667 1_4:-436827 None:intergenic
CGATGAGTACATGTTACTCT+TGG 0.511451 1_4:-438742 None:intergenic
AACTTCTCCATGGCTTGAGC+TGG 0.517646 1_4:-439396 None:intergenic
AATAATTGTTCCTTTGGTGT+CGG 0.522248 1_4:-438782 None:intergenic
TGCCATACCGGTGAGATTGA+AGG 0.523692 1_4:-438691 None:intergenic
AACTAATGGAAAATCCGTTA+GGG 0.528322 1_4:+439082 Msa0141730:CDS
GGAGATGTTGACTTTGGTTG+GGG 0.528669 1_4:+439252 Msa0141730:CDS
AACGATAAGACATTATATGA+TGG 0.532826 1_4:-439724 None:intergenic
GGAGGAGTTGGTGCTATACC+TGG 0.535378 1_4:+439303 Msa0141730:CDS
CACACATTGTATGGTTTAGA+CGG 0.536195 1_4:-438606 None:intergenic
CATGTATTCTTGAGTTACAT+CGG 0.537828 1_4:-439136 None:intergenic
GCACCAACTCCTCCTTTAGC+TGG 0.539177 1_4:-439294 None:intergenic
GTATATGGTGGACCAGCTAA+AGG 0.539427 1_4:+439282 Msa0141730:CDS
GCCTTAGGTGAAATTTCTTG+CGG 0.540414 1_4:+438658 Msa0141730:CDS
ATTATTGCAGCAGCACTATG+TGG 0.541966 1_4:+436337 None:intergenic
CTCTTTGACGAACTTCTCCA+TGG 0.543009 1_4:-439406 None:intergenic
AATCTCACCGGTATGGCATA+GGG 0.546127 1_4:+438696 Msa0141730:CDS
TCCACCATAAGTTTCCTGTC+AGG 0.550793 1_4:-436725 None:intergenic
GGTTAAGGGAAGGTCCTGAC+AGG 0.552476 1_4:+436711 Msa0141730:CDS
TTGTTTACCAGCTCAAGCCA+TGG 0.552757 1_4:+439389 Msa0141730:CDS
AGAAGCTCCCTATGCCATAC+CGG 0.552944 1_4:-438703 None:intergenic
GGACCAGCTAAAGGAGGAGT+TGG 0.553044 1_4:+439291 Msa0141730:CDS
ATAGGGAGCTTCTAAATGCA+AGG 0.554177 1_4:+438713 Msa0141730:CDS
CAGATATAGATGACCAAGAA+GGG 0.559168 1_4:+436561 Msa0141730:five_prime_UTR
ATAGAGATTGGGCCATAATG+AGG 0.559544 1_4:-436462 None:intergenic
TTTATTATCCATTTGCAGGT+AGG 0.559740 1_4:+436690 Msa0141730:CDS
AGTGGTTATTATAGAAGGTA+CGG 0.560178 1_4:-436404 None:intergenic
ATTAACGAACCTACTATTGG+TGG 0.562257 1_4:+439453 Msa0141730:CDS
TCAGATATAGATGACCAAGA+AGG 0.565827 1_4:+436560 Msa0141730:five_prime_UTR
AGCGCGAATTGTGGCTACCT+CGG 0.567132 1_4:-438848 None:intergenic
AACCTTCAATCTCACCGGTA+TGG 0.568331 1_4:+438689 Msa0141730:CDS
CCCGCAAGAAATTTCACCTA+AGG 0.570019 1_4:-438659 None:intergenic
CTCTATCATTCACAGTGAAG+AGG 0.571132 1_4:+436479 Msa0141730:five_prime_UTR
CCGTCGCGGTTACAACCGCT+GGG 0.572108 1_4:+439058 Msa0141730:CDS
AGTATGATCCAAATATGGCA+GGG 0.572294 1_4:+436618 Msa0141730:exon
AACTCTAGTGCATACCCTAA+CGG 0.573134 1_4:-439096 None:intergenic
CCGTTACCAAATGGTTACTA+CGG 0.574521 1_4:+439018 Msa0141730:CDS
AGAATACATGCATTCAGTTG+CGG 0.574989 1_4:+439149 Msa0141730:CDS
AATTATTCCCTTAGATGACA+TGG 0.576689 1_4:+438798 Msa0141730:CDS
CATACAATGTGTGACGCAGC+AGG 0.579280 1_4:+438616 Msa0141730:CDS
CGATTGTACGACAACGCCAA+AGG 0.587072 1_4:-438924 None:intergenic
AATACCTGAATCACCAACAA+TGG 0.589517 1_4:-436890 None:intergenic
ACATTAGTTTAAGGTCTACA+AGG 0.590944 1_4:-439502 None:intergenic
CGATGGGCCATGTCATCTAA+GGG 0.603002 1_4:-438805 None:intergenic
AAGGAAGAAACAATGAAACT+AGG 0.603955 1_4:-439483 None:intergenic
TTATTGCAGCAGCACTATGT+GGG 0.605382 1_4:+436338 None:intergenic
GCGGATTTAATGGTTATCAA+GGG 0.606067 1_4:+439168 Msa0141730:CDS
ATCTGCATTGTCAATGCTCG+TGG 0.609350 1_4:+438982 Msa0141730:CDS
CCGTAGTAACCATTTGGTAA+CGG 0.610295 1_4:-439018 None:intergenic
ATATAAAAGCTAGCAACACC+AGG 0.611199 1_4:-439321 None:intergenic
TCTCACACCAATCATAGCAA+AGG 0.613641 1_4:-439537 None:intergenic
GAACGAACCTTCAATCTCAC+CGG 0.615082 1_4:+438684 Msa0141730:CDS
TGGGAGAAGAGCGCGAATTG+TGG 0.616656 1_4:-438857 None:intergenic
CTTCTAACCACAGTAAAATG+TGG 0.618528 1_4:-439195 None:intergenic
AAAAGTAGCTCATCCAAACA+AGG 0.619216 1_4:-436833 None:intergenic
TGAGGGTCTTACAAATTCAG+GGG 0.622901 1_4:-436506 None:intergenic
CCCAGCGGTTGTAACCGCGA+CGG 0.625128 1_4:-439058 None:intergenic
ATGGTGGATTGTAATGATGA+AGG 0.626655 1_4:+436740 Msa0141730:CDS
GATTGGGCCATAATGAGGAA+AGG 0.628876 1_4:-436457 None:intergenic
GAACAAGTTCCCGACACCAA+AGG 0.634936 1_4:+438772 Msa0141730:CDS
GCTTGAGCTGGTAAACAAAG+TGG 0.635861 1_4:-439384 None:intergenic
TCATCCAAACAAGGAAAAGG+AGG 0.640809 1_4:-436824 None:intergenic
TAGGGAGCTTCTAAATGCAA+GGG 0.644953 1_4:+438714 Msa0141730:CDS
TTTGCAGGTAGGTTAAGGGA+AGG 0.651163 1_4:+436701 Msa0141730:CDS
TAGTAACCATTTGGTAACGG+TGG 0.653193 1_4:-439015 None:intergenic
TAGGTAACACGTCTCAAGTG+TGG 0.654765 1_4:+438565 Msa0141730:intron
AACGCCAAAGGCATGCGGTG+AGG 0.656317 1_4:-438912 None:intergenic
TCCTGACAGGAAACTTATGG+TGG 0.657173 1_4:+436724 Msa0141730:CDS
TGGGGAAAAGCTGTATATGG+TGG 0.663720 1_4:+439270 Msa0141730:CDS
TATGGTGGACCAGCTAAAGG+AGG 0.665823 1_4:+439285 Msa0141730:CDS
TTAAGCACTTCTGAAGAACC+AGG 0.667440 1_4:-436863 None:intergenic
GTGGGTTATCCCAATGCATG+TGG 0.683744 1_4:+436356 Msa0141730:five_prime_UTR
GTTGCAAAAGATAATCACGA+TGG 0.685988 1_4:+439573 Msa0141730:three_prime_UTR
GTAACACGTCTCAAGTGTGG+TGG 0.692143 1_4:+438568 Msa0141730:CDS
ACGACAACGCCAAAGGCATG+CGG 0.713965 1_4:-438917 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTCTTTTGAAAAATTAA+CGG - chr1_4:437091-437110 None:intergenic 10.0%
!! AAATAGATAACTTTATATTA+TGG + chr1_4:437106-437125 Msa0141730:intron 10.0%
!!! TATTTCTTTTGAAAAATTAA+CGG - chr1_4:437091-437110 None:intergenic 10.0%
!! AAATAGATAACTTTATATTA+TGG + chr1_4:437106-437125 Msa0141730:intron 10.0%
!!! ATTATTTTAGGTTATTATTT+TGG - chr1_4:437683-437702 None:intergenic 10.0%
!!! TAACCTAAAATAATTTTTTA+AGG + chr1_4:437689-437708 Msa0141730:intron 10.0%
!!! TTTCCTTAAAAAATTATTTT+AGG - chr1_4:437695-437714 None:intergenic 10.0%
!!! ATTATCATTTTTCATTTTTT+TGG + chr1_4:437868-437887 Msa0141730:intron 10.0%
!!! TTATCATTTTTCATTTTTTT+GGG + chr1_4:437869-437888 Msa0141730:intron 10.0%
!! TATATTTCATAAATTTACAG+TGG + chr1_4:436958-436977 Msa0141730:intron 15.0%
!!! ATTTTGGACTTTTTTTTTTT+TGG + chr1_4:437461-437480 Msa0141730:intron 15.0%
!!! TTTTATGCAATTAAATAAGA+TGG - chr1_4:437794-437813 None:intergenic 15.0%
!! TATTTAATTGCATAAAAAAC+TGG + chr1_4:437797-437816 Msa0141730:intron 15.0%
!!! TATCATTTTTCATTTTTTTG+GGG + chr1_4:437870-437889 Msa0141730:intron 15.0%
!! TCAAAAAAAAAAAAAAGAGT+GGG + chr1_4:438213-438232 Msa0141730:intron 15.0%
!! TACTTATAAAATTGTATCAC+TGG - chr1_4:436602-436621 None:intergenic 20.0%
!! TTATAAGTATGATCCAAATA+TGG + chr1_4:436613-436632 Msa0141730:exon 20.0%
!!! CTTTTCTAATAATATCAACA+GGG - chr1_4:436649-436668 None:intergenic 20.0%
!! TGAAACAATATTTAAATCCT+AGG + chr1_4:437607-437626 Msa0141730:intron 20.0%
!!! ATCATTTTTCATTTTTTTGG+GGG + chr1_4:437871-437890 Msa0141730:intron 20.0%
!! CTTACATGTAAATTTGTAAA+TGG + chr1_4:437897-437916 Msa0141730:intron 20.0%
!!! TGGCACATATATTTTTTTAA+AGG + chr1_4:437917-437936 Msa0141730:intron 20.0%
!!! CAAGCACAAATAATTTTTTT+GGG + chr1_4:438012-438031 Msa0141730:intron 20.0%
!! AAAAAAATTTAATTCAGCTG+AGG + chr1_4:438136-438155 Msa0141730:intron 20.0%
!! GTCAAAAAAAAAAAAAAGAG+TGG + chr1_4:438212-438231 Msa0141730:intron 20.0%
!! TAGACAAAATTTATACATCA+CGG + chr1_4:438385-438404 Msa0141730:intron 20.0%
!!! AACTTTAACAACATTTTTCA+CGG - chr1_4:439639-439658 None:intergenic 20.0%
!! TAAACGAACTTATGATAATA+AGG - chr1_4:439817-439836 None:intergenic 20.0%
!!! TTGATAGTGGTTATTATAGA+AGG - chr1_4:436412-436431 None:intergenic 25.0%
!! GAAAGGTATAATTGAAGAAA+GGG - chr1_4:436443-436462 None:intergenic 25.0%
! AATTATACCTTTCCTCATTA+TGG + chr1_4:436450-436469 Msa0141730:five_prime_UTR 25.0%
!! GCTTTTCTAATAATATCAAC+AGG - chr1_4:436650-436669 None:intergenic 25.0%
!! GATGTTAGTTTGAAAGAATT+TGG + chr1_4:436788-436807 Msa0141730:CDS 25.0%
! ATATAGTACAAGTGTATGTA+CGG - chr1_4:437015-437034 None:intergenic 25.0%
! ATATAGTACGAGTATATGTT+GGG - chr1_4:437061-437080 None:intergenic 25.0%
! AATATAGTACGAGTATATGT+TGG - chr1_4:437062-437081 None:intergenic 25.0%
! CATATACTCGTACTATATTA+AGG + chr1_4:437063-437082 Msa0141730:intron 25.0%
!!! AACTTAAGTTGGTTAGATTT+TGG + chr1_4:437445-437464 Msa0141730:intron 25.0%
!!! GACTTTTTTTTTTTTGGTAG+TGG + chr1_4:437467-437486 Msa0141730:intron 25.0%
! TTTCTATCGAATAGAATCTT+AGG + chr1_4:437654-437673 Msa0141730:intron 25.0%
! ATGCAATTAAATAAGATGGT+AGG - chr1_4:437790-437809 None:intergenic 25.0%
!! GTTCACAAACAAAATTTTAC+AGG + chr1_4:437989-438008 Msa0141730:intron 25.0%
!!! GCAAGCACAAATAATTTTTT+TGG + chr1_4:438011-438030 Msa0141730:intron 25.0%
! AAAAAAAAAAAAAGAGTGGG+TGG + chr1_4:438216-438235 Msa0141730:intron 25.0%
! AAAAAAAAAAAAGAGTGGGT+GGG + chr1_4:438217-438236 Msa0141730:intron 25.0%
! ATACCTTTCAAAAATGCTAA+AGG + chr1_4:439342-439361 Msa0141730:CDS 25.0%
!! TTCAAACCAACATTAGTTTA+AGG - chr1_4:439514-439533 None:intergenic 25.0%
!! GGTATTTAGCTCAATTAATA+AGG - chr1_4:439617-439636 None:intergenic 25.0%
!! ACTTTAACAACATTTTTCAC+GGG - chr1_4:439638-439657 None:intergenic 25.0%
! AACGATAAGACATTATATGA+TGG - chr1_4:439727-439746 None:intergenic 25.0%
AGTGGTTATTATAGAAGGTA+CGG - chr1_4:436407-436426 None:intergenic 30.0%
!! AAGGGTACTAAATTTGATAG+TGG - chr1_4:436425-436444 None:intergenic 30.0%
! GGAAAGGTATAATTGAAGAA+AGG - chr1_4:436444-436463 None:intergenic 30.0%
! GAGAGTAATTTTACTTCATG+AGG - chr1_4:436542-436561 None:intergenic 30.0%
!! GTGTTTTATTATCCATTTGC+AGG + chr1_4:436686-436705 Msa0141730:CDS 30.0%
TTTATTATCCATTTGCAGGT+AGG + chr1_4:436690-436709 Msa0141730:CDS 30.0%
AACTTAGACACAACTTAAGT+TGG + chr1_4:437434-437453 Msa0141730:intron 30.0%
!!! TTTTTTTTTTTGGTAGTGGC+TGG + chr1_4:437471-437490 Msa0141730:intron 30.0%
!!! TTTTTTTTTTGGTAGTGGCT+GGG + chr1_4:437472-437491 Msa0141730:intron 30.0%
! ACACTTAGATTTTGGACTAA+TGG + chr1_4:437559-437578 Msa0141730:intron 30.0%
! TTGATTGAAACGACACATTT+TGG - chr1_4:438244-438263 None:intergenic 30.0%
!! CTTGAATTTTGACCATTTGT+AGG + chr1_4:438546-438565 Msa0141730:intron 30.0%
! AATAATTGTTCCTTTGGTGT+CGG - chr1_4:438785-438804 None:intergenic 30.0%
TAAGGGAATAATTGTTCCTT+TGG - chr1_4:438791-438810 None:intergenic 30.0%
AATTATTCCCTTAGATGACA+TGG + chr1_4:438798-438817 Msa0141730:CDS 30.0%
!!! CGAAATTGGATTGTTTTTGT+TGG - chr1_4:438888-438907 None:intergenic 30.0%
AAACTAATGGAAAATCCGTT+AGG + chr1_4:439081-439100 Msa0141730:CDS 30.0%
AACTAATGGAAAATCCGTTA+GGG + chr1_4:439082-439101 Msa0141730:CDS 30.0%
CATGTATTCTTGAGTTACAT+CGG - chr1_4:439139-439158 None:intergenic 30.0%
! TCTCCTTTAGCATTTTTGAA+AGG - chr1_4:439348-439367 None:intergenic 30.0%
TTTCAAAAATGCTAAAGGAG+AGG + chr1_4:439347-439366 Msa0141730:CDS 30.0%
AACATTAACGAACCTACTAT+TGG + chr1_4:439450-439469 Msa0141730:CDS 30.0%
! GAAACAATGAAACTAGGTTT+TGG - chr1_4:439480-439499 None:intergenic 30.0%
AAGGAAGAAACAATGAAACT+AGG - chr1_4:439486-439505 None:intergenic 30.0%
! ACATTAGTTTAAGGTCTACA+AGG - chr1_4:439505-439524 None:intergenic 30.0%
TGTAGACCTTAAACTAATGT+TGG + chr1_4:439505-439524 Msa0141730:exon 30.0%
! TGAATTTCCTTTGCTATGAT+TGG + chr1_4:439530-439549 Msa0141730:three_prime_UTR 30.0%
CACTGTGAATGATAGAGATT+GGG - chr1_4:436476-436495 None:intergenic 35.0%
TCACTGTGAATGATAGAGAT+TGG - chr1_4:436477-436496 None:intergenic 35.0%
TTGAGGGTCTTACAAATTCA+GGG - chr1_4:436510-436529 None:intergenic 35.0%
TTTGAGGGTCTTACAAATTC+AGG - chr1_4:436511-436530 None:intergenic 35.0%
!!! CATGAGGTGTTTTGTTTTGA+GGG - chr1_4:436526-436545 None:intergenic 35.0%
!!! TCATGAGGTGTTTTGTTTTG+AGG - chr1_4:436527-436546 None:intergenic 35.0%
TCAGATATAGATGACCAAGA+AGG + chr1_4:436560-436579 Msa0141730:five_prime_UTR 35.0%
CAGATATAGATGACCAAGAA+GGG + chr1_4:436561-436580 Msa0141730:five_prime_UTR 35.0%
TTTGAAAACGTAACCCTTCT+TGG - chr1_4:436577-436596 None:intergenic 35.0%
AAGTATGATCCAAATATGGC+AGG + chr1_4:436617-436636 Msa0141730:exon 35.0%
AGTATGATCCAAATATGGCA+GGG + chr1_4:436618-436637 Msa0141730:exon 35.0%
!! ATGGTGGATTGTAATGATGA+AGG + chr1_4:436740-436759 Msa0141730:CDS 35.0%
!!! TGAAGGTGTTTTGTTCATTG+AGG + chr1_4:436757-436776 Msa0141730:CDS 35.0%
AAAAGTAGCTCATCCAAACA+AGG - chr1_4:436836-436855 None:intergenic 35.0%
AATACCTGAATCACCAACAA+TGG - chr1_4:436893-436912 None:intergenic 35.0%
!!! TTTTTTTTTGGTAGTGGCTG+GGG + chr1_4:437473-437492 Msa0141730:intron 35.0%
GACAATGCATAAAGTATGCA+AGG - chr1_4:437512-437531 None:intergenic 35.0%
! TGCAAGACACTTTTATTCCT+AGG - chr1_4:437627-437646 None:intergenic 35.0%
AAATGATTCAAGTGAGGTCA+AGG - chr1_4:438084-438103 None:intergenic 35.0%
CTACACAAATGATTCAAGTG+AGG - chr1_4:438090-438109 None:intergenic 35.0%
CTCACTTGAATCATTTGTGT+AGG + chr1_4:438088-438107 Msa0141730:intron 35.0%
! TCTTTGTTTCCTAGAAAGAG+TGG + chr1_4:438184-438203 Msa0141730:intron 35.0%
! CTTTGTTTCCTAGAAAGAGT+GGG + chr1_4:438185-438204 Msa0141730:intron 35.0%
TTTGTTTCCTAGAAAGAGTG+GGG + chr1_4:438186-438205 Msa0141730:intron 35.0%
AGACACACACTAGAGTTAAA+AGG - chr1_4:438283-438302 None:intergenic 35.0%
CACAACAAGTGTTTCATTTG+TGG + chr1_4:438470-438489 Msa0141730:intron 35.0%
CACACATTGTATGGTTTAGA+CGG - chr1_4:438609-438628 None:intergenic 35.0%
!! ATAATTGTTCCTTTGGTGTC+GGG - chr1_4:438784-438803 None:intergenic 35.0%
!!! ATTGTTTTTGTTGGTGAGGT+GGG - chr1_4:438879-438898 None:intergenic 35.0%
!!! TTGGATTGTTTTTGTTGGTG+AGG - chr1_4:438883-438902 None:intergenic 35.0%
TTTAATCCACCGTTACCAAA+TGG + chr1_4:439009-439028 Msa0141730:CDS 35.0%
!! ATTTTCCATTAGTTTCCCAG+CGG - chr1_4:439076-439095 None:intergenic 35.0%
AGAATACATGCATTCAGTTG+CGG + chr1_4:439149-439168 Msa0141730:CDS 35.0%
TGCGGATTTAATGGTTATCA+AGG + chr1_4:439167-439186 Msa0141730:CDS 35.0%
GCGGATTTAATGGTTATCAA+GGG + chr1_4:439168-439187 Msa0141730:CDS 35.0%
CTTCTAACCACAGTAAAATG+TGG - chr1_4:439198-439217 None:intergenic 35.0%
ATATAAAAGCTAGCAACACC+AGG - chr1_4:439324-439343 None:intergenic 35.0%
AAAAATGCTAAAGGAGAGGA+AGG + chr1_4:439351-439370 Msa0141730:CDS 35.0%
ATTAACGAACCTACTATTGG+TGG + chr1_4:439453-439472 Msa0141730:CDS 35.0%
GTTGCAAAAGATAATCACGA+TGG + chr1_4:439573-439592 Msa0141730:three_prime_UTR 35.0%
TATAATGCGCCACATGCATT+GGG - chr1_4:436368-436387 None:intergenic 40.0%
ATATAATGCGCCACATGCAT+TGG - chr1_4:436369-436388 None:intergenic 40.0%
ATAGAGATTGGGCCATAATG+AGG - chr1_4:436465-436484 None:intergenic 40.0%
CTCTATCATTCACAGTGAAG+AGG + chr1_4:436479-436498 Msa0141730:five_prime_UTR 40.0%
TGAGGGTCTTACAAATTCAG+GGG - chr1_4:436509-436528 None:intergenic 40.0%
ATCCATTTGCAGGTAGGTTA+AGG + chr1_4:436696-436715 Msa0141730:CDS 40.0%
TCCATTTGCAGGTAGGTTAA+GGG + chr1_4:436697-436716 Msa0141730:CDS 40.0%
! TCAACCTCCTTTTCCTTGTT+TGG + chr1_4:436820-436839 Msa0141730:CDS 40.0%
TCATCCAAACAAGGAAAAGG+AGG - chr1_4:436827-436846 None:intergenic 40.0%
AGCTCATCCAAACAAGGAAA+AGG - chr1_4:436830-436849 None:intergenic 40.0%
!!! GAGCTACTTTTTGATGTTCC+TGG + chr1_4:436845-436864 Msa0141730:CDS 40.0%
! TTAAGCACTTCTGAAGAACC+AGG - chr1_4:436866-436885 None:intergenic 40.0%
AGTGCTTAACTCTCCATTGT+TGG + chr1_4:436877-436896 Msa0141730:CDS 40.0%
CAAGTGTATGTACGGATTTG+CGG - chr1_4:437007-437026 None:intergenic 40.0%
! TGAGTTTAGCTCAGTTGGTT+GGG - chr1_4:437534-437553 None:intergenic 40.0%
! TCACGAGGACACTTAGATTT+TGG + chr1_4:437551-437570 Msa0141730:intron 40.0%
TCACGGATCAAGCTATTACA+TGG + chr1_4:438402-438421 Msa0141730:intron 40.0%
GGATCAAGCTATTACATGGT+TGG + chr1_4:438406-438425 Msa0141730:intron 40.0%
TGAGACGTGTTACCTACAAA+TGG - chr1_4:438561-438580 None:intergenic 40.0%
GCCTTAGGTGAAATTTCTTG+CGG + chr1_4:438658-438677 Msa0141730:CDS 40.0%
CCTTAGGTGAAATTTCTTGC+GGG + chr1_4:438659-438678 Msa0141730:CDS 40.0%
ATAGGGAGCTTCTAAATGCA+AGG + chr1_4:438713-438732 Msa0141730:CDS 40.0%
TAGGGAGCTTCTAAATGCAA+GGG + chr1_4:438714-438733 Msa0141730:CDS 40.0%
TGAGTACATGTTACTCTTGG+TGG - chr1_4:438742-438761 None:intergenic 40.0%
CGATGAGTACATGTTACTCT+TGG - chr1_4:438745-438764 None:intergenic 40.0%
ACCGAAGAAAAAAGAGCGAT+GGG - chr1_4:438824-438843 None:intergenic 40.0%
!!! GATTGTTTTTGTTGGTGAGG+TGG - chr1_4:438880-438899 None:intergenic 40.0%
!! TAGTAACCATTTGGTAACGG+TGG - chr1_4:439018-439037 None:intergenic 40.0%
CCGTAGTAACCATTTGGTAA+CGG - chr1_4:439021-439040 None:intergenic 40.0%
CCGTTACCAAATGGTTACTA+CGG + chr1_4:439018-439037 Msa0141730:CDS 40.0%
GCATTACCGTAGTAACCATT+TGG - chr1_4:439027-439046 None:intergenic 40.0%
AACTCTAGTGCATACCCTAA+CGG - chr1_4:439099-439118 None:intergenic 40.0%
GCATTCAGTTGCGGATTTAA+TGG + chr1_4:439158-439177 Msa0141730:CDS 40.0%
!! GGATTTGGAGATGTTGACTT+TGG + chr1_4:439246-439265 Msa0141730:CDS 40.0%
!! TTGGAGATGTTGACTTTGGT+TGG + chr1_4:439250-439269 Msa0141730:CDS 40.0%
!! TGGAGATGTTGACTTTGGTT+GGG + chr1_4:439251-439270 Msa0141730:CDS 40.0%
TCTCACACCAATCATAGCAA+AGG - chr1_4:439540-439559 None:intergenic 40.0%
GATTGGGCCATAATGAGGAA+AGG - chr1_4:436460-436479 None:intergenic 45.0%
TCCCTTAACCTACCTGCAAA+TGG - chr1_4:436701-436720 None:intergenic 45.0%
TTTGCAGGTAGGTTAAGGGA+AGG + chr1_4:436701-436720 Msa0141730:CDS 45.0%
AGGTCCTGACAGGAAACTTA+TGG + chr1_4:436721-436740 Msa0141730:CDS 45.0%
TCCTGACAGGAAACTTATGG+TGG + chr1_4:436724-436743 Msa0141730:CDS 45.0%
TCCACCATAAGTTTCCTGTC+AGG - chr1_4:436728-436747 None:intergenic 45.0%
!! CTCTCCATTGTTGGTGATTC+AGG + chr1_4:436886-436905 Msa0141730:CDS 45.0%
CGAGTATATGTTGGGATGAG+TGG - chr1_4:437053-437072 None:intergenic 45.0%
CATAAAGTATGCAAGGTCCG+AGG - chr1_4:437505-437524 None:intergenic 45.0%
! GTGAGTTTAGCTCAGTTGGT+TGG - chr1_4:437535-437554 None:intergenic 45.0%
TGACAAACCCCACTCTTTCT+AGG - chr1_4:438196-438215 None:intergenic 45.0%
TAGGTAACACGTCTCAAGTG+TGG + chr1_4:438565-438584 Msa0141730:intron 45.0%
GTCCAATTCATGAGTGCCTT+AGG + chr1_4:438643-438662 Msa0141730:CDS 45.0%
! CACCTAAGGCACTCATGAAT+TGG - chr1_4:438648-438667 None:intergenic 45.0%
CCCGCAAGAAATTTCACCTA+AGG - chr1_4:438662-438681 None:intergenic 45.0%
GAACGAACCTTCAATCTCAC+CGG + chr1_4:438684-438703 Msa0141730:CDS 45.0%
AACCTTCAATCTCACCGGTA+TGG + chr1_4:438689-438708 Msa0141730:CDS 45.0%
AATCTCACCGGTATGGCATA+GGG + chr1_4:438696-438715 Msa0141730:CDS 45.0%
!!! GCCCATCGCTCTTTTTTCTT+CGG + chr1_4:438820-438839 Msa0141730:CDS 45.0%
GACCGAAGAAAAAAGAGCGA+TGG - chr1_4:438825-438844 None:intergenic 45.0%
!!! TTTTTTCTTCGGTCCAACCG+AGG + chr1_4:438831-438850 Msa0141730:CDS 45.0%
TGCGGTGAGGATTTCGAAAT+TGG - chr1_4:438902-438921 None:intergenic 45.0%
ATCTGCATTGTCAATGCTCG+TGG + chr1_4:438982-439001 Msa0141730:CDS 45.0%
TACAACCGCTGGGAAACTAA+TGG + chr1_4:439068-439087 Msa0141730:CDS 45.0%
! GGGTAGACCACATTTTACTG+TGG + chr1_4:439188-439207 Msa0141730:CDS 45.0%
!! GGAGATGTTGACTTTGGTTG+GGG + chr1_4:439252-439271 Msa0141730:CDS 45.0%
GGTTGGGGAAAAGCTGTATA+TGG + chr1_4:439267-439286 Msa0141730:CDS 45.0%
TGGGGAAAAGCTGTATATGG+TGG + chr1_4:439270-439289 Msa0141730:CDS 45.0%
!! GTATATGGTGGACCAGCTAA+AGG + chr1_4:439282-439301 Msa0141730:CDS 45.0%
TGCTAAAGGAGAGGAAGGTT+TGG + chr1_4:439356-439375 Msa0141730:CDS 45.0%
GCTTGAGCTGGTAAACAAAG+TGG - chr1_4:439387-439406 None:intergenic 45.0%
TTGTTTACCAGCTCAAGCCA+TGG + chr1_4:439389-439408 Msa0141730:CDS 45.0%
CTCTTTGACGAACTTCTCCA+TGG - chr1_4:439409-439428 None:intergenic 45.0%
!!! GGTTTTGGACCACCAATAGT+AGG - chr1_4:439465-439484 None:intergenic 45.0%
!! ATAAATTAATTAGTTAAAAT+TGG - chr1_4:438497-438516 None:intergenic 5.0%
! GTGGGTTATCCCAATGCATG+TGG + chr1_4:436356-436375 Msa0141730:five_prime_UTR 50.0%
GGGTCTTTCCCTGCCATATT+TGG - chr1_4:436629-436648 None:intergenic 50.0%
!!! TTTTGGTAGTGGCTGGGGTT+TGG + chr1_4:437478-437497 Msa0141730:intron 50.0%
! CCTCGTGAGTTTAGCTCAGT+TGG - chr1_4:437539-437558 None:intergenic 50.0%
CCAACTGAGCTAAACTCACG+AGG + chr1_4:437536-437555 Msa0141730:intron 50.0%
GTAACACGTCTCAAGTGTGG+TGG + chr1_4:438568-438587 Msa0141730:CDS 50.0%
CTGCTGCGTCACACATTGTA+TGG - chr1_4:438618-438637 None:intergenic 50.0%
CATACAATGTGTGACGCAGC+AGG + chr1_4:438616-438635 Msa0141730:CDS 50.0%
AATGTGTGACGCAGCAGGTT+TGG + chr1_4:438621-438640 Msa0141730:CDS 50.0%
TGCCATACCGGTGAGATTGA+AGG - chr1_4:438694-438713 None:intergenic 50.0%
CAATCTCACCGGTATGGCAT+AGG + chr1_4:438695-438714 Msa0141730:CDS 50.0%
AGAAGCTCCCTATGCCATAC+CGG - chr1_4:438706-438725 None:intergenic 50.0%
GAACAAGTTCCCGACACCAA+AGG + chr1_4:438772-438791 Msa0141730:CDS 50.0%
CGATGGGCCATGTCATCTAA+GGG - chr1_4:438808-438827 None:intergenic 50.0%
AAATCCTCACCGCATGCCTT+TGG + chr1_4:438908-438927 Msa0141730:CDS 50.0%
CGATTGTACGACAACGCCAA+AGG - chr1_4:438927-438946 None:intergenic 50.0%
CGAACTTCTTCGTCAGTGTC+TGG - chr1_4:438958-438977 None:intergenic 50.0%
GATGTGACACGTGCTGGATT+TGG + chr1_4:439231-439250 Msa0141730:CDS 50.0%
!! TATGGTGGACCAGCTAAAGG+AGG + chr1_4:439285-439304 Msa0141730:CDS 50.0%
AACTTCTCCATGGCTTGAGC+TGG - chr1_4:439399-439418 None:intergenic 50.0%
GGTTAAGGGAAGGTCCTGAC+AGG + chr1_4:436711-436730 Msa0141730:CDS 55.0%
GCGATGGGCCATGTCATCTA+AGG - chr1_4:438809-438828 None:intergenic 55.0%
CGAATTGTGGCTACCTCGGT+TGG - chr1_4:438847-438866 None:intergenic 55.0%
AGCGCGAATTGTGGCTACCT+CGG - chr1_4:438851-438870 None:intergenic 55.0%
! TGGGAGAAGAGCGCGAATTG+TGG - chr1_4:438860-438879 None:intergenic 55.0%
! ACGACAACGCCAAAGGCATG+CGG - chr1_4:438920-438939 None:intergenic 55.0%
! TGCTTTTGCGTTTCCCGTCG+CGG + chr1_4:439044-439063 Msa0141730:CDS 55.0%
GTGTCTGATGTGACACGTGC+TGG + chr1_4:439225-439244 Msa0141730:CDS 55.0%
GGACCAGCTAAAGGAGGAGT+TGG + chr1_4:439291-439310 Msa0141730:CDS 55.0%
GCACCAACTCCTCCTTTAGC+TGG - chr1_4:439297-439316 None:intergenic 55.0%
!! GGAGGAGTTGGTGCTATACC+TGG + chr1_4:439303-439322 Msa0141730:CDS 55.0%
AGTGGCTGGGGTTTGGACCT+CGG + chr1_4:437485-437504 Msa0141730:intron 60.0%
! AACGCCAAAGGCATGCGGTG+AGG - chr1_4:438915-438934 None:intergenic 60.0%
CCAGCGGTTGTAACCGCGAC+GGG - chr1_4:439060-439079 None:intergenic 65.0%
CCCAGCGGTTGTAACCGCGA+CGG - chr1_4:439061-439080 None:intergenic 65.0%
CCGTCGCGGTTACAACCGCT+GGG + chr1_4:439058-439077 Msa0141730:CDS 65.0%
CCCGTCGCGGTTACAACCGC+TGG + chr1_4:439057-439076 Msa0141730:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 436340 439840 436340 ID=Msa0141730;Name=Msa0141730
chr1_4 mRNA 436340 439840 436340 ID=Msa0141730-mRNA-1;Parent=Msa0141730;Name=Msa0141730-mRNA-1;_AED=0.14;_eAED=0.14;_QI=292|1|1|1|1|1|2|331|405
chr1_4 exon 436340 436907 436340 ID=Msa0141730-mRNA-1:exon:43;Parent=Msa0141730-mRNA-1
chr1_4 exon 438568 439840 438568 ID=Msa0141730-mRNA-1:exon:44;Parent=Msa0141730-mRNA-1
chr1_4 five_prime_UTR 436340 436631 436340 ID=Msa0141730-mRNA-1:five_prime_utr;Parent=Msa0141730-mRNA-1
chr1_4 CDS 436632 436907 436632 ID=Msa0141730-mRNA-1:cds;Parent=Msa0141730-mRNA-1
chr1_4 CDS 438568 439509 438568 ID=Msa0141730-mRNA-1:cds;Parent=Msa0141730-mRNA-1
chr1_4 three_prime_UTR 439510 439840 439510 ID=Msa0141730-mRNA-1:three_prime_utr;Parent=Msa0141730-mRNA-1
Gene Sequence

>Msa0141730

ATGGCAGGGAAAGACCCTGTTGATATTATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGGTTAAGGGAAGGTCCTGACAGGAAACTTATGGTGGATTGTAATGATGAAGGTGTTTTGTTCATTGAGGCTGATGCTGATGTTAGTTTGAAAGAATTTGGTGATGCTCTTCAACCTCCTTTTCCTTGTTTGGATGAGCTACTTTTTGATGTTCCTGGTTCTTCAGAAGTGCTTAACTCTCCATTGTTGGTGATTCAGGTAACACGTCTCAAGTGTGGTGGTTTCATTTTTGCTCTCCGTCTAAACCATACAATGTGTGACGCAGCAGGTTTGGTCCAATTCATGAGTGCCTTAGGTGAAATTTCTTGCGGGATGAACGAACCTTCAATCTCACCGGTATGGCATAGGGAGCTTCTAAATGCAAGGGATCCACCAAGAGTAACATGTACTCATCGTGAATATGAACAAGTTCCCGACACCAAAGGAACAATTATTCCCTTAGATGACATGGCCCATCGCTCTTTTTTCTTCGGTCCAACCGAGGTAGCCACAATTCGCGCTCTTCTCCCACCTCACCAACAAAAACAATCCAATTTCGAAATCCTCACCGCATGCCTTTGGCGTTGTCGTACAATCGCATTACAACCAGACACTGACGAAGAAGTTCGCATAATCTGCATTGTCAATGCTCGTGGCAAGTTTAATCCACCGTTACCAAATGGTTACTACGGTAATGCTTTTGCGTTTCCCGTCGCGGTTACAACCGCTGGGAAACTAATGGAAAATCCGTTAGGGTATGCACTAGAGTTAGTTAAAAAAGCAAAATCCGATGTAACTCAAGAATACATGCATTCAGTTGCGGATTTAATGGTTATCAAGGGTAGACCACATTTTACTGTGGTTAGAAGTTATCTAGTGTCTGATGTGACACGTGCTGGATTTGGAGATGTTGACTTTGGTTGGGGAAAAGCTGTATATGGTGGACCAGCTAAAGGAGGAGTTGGTGCTATACCTGGTGTTGCTAGCTTTTATATACCTTTCAAAAATGCTAAAGGAGAGGAAGGTTTGGTTATACCACTTTGTTTACCAGCTCAAGCCATGGAGAAGTTCGTCAAAGAGTTAGATAGTGTACTTAAGAACAACATTAACGAACCTACTATTGGTGGTCCAAAACCTAGTTTCATTGTTTCTTCCTTGTAG
Protein sequence

>Msa0141730

MAGKDPVDIIRKALAKTLVFYYPFAGRLREGPDRKLMVDCNDEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNSPLLVIQVTRLKCGGFIFALRLNHTMCDAAGLVQFMSALGEISCGMNEPSISPVWHRELLNARDPPRVTCTHREYEQVPDTKGTIIPLDDMAHRSFFFGPTEVATIRALLPPHQQKQSNFEILTACLWRCRTIALQPDTDEEVRIICIVNARGKFNPPLPNGYYGNAFAFPVAVTTAGKLMENPLGYALELVKKAKSDVTQEYMHSVADLMVIKGRPHFTVVRSYLVSDVTRAGFGDVDFGWGKAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKELDSVLKNNINEPTIGGPKPSFIVSSL*