AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0184440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184440 MtrunA17_Chr1g0165671 96.646 477 16 0 15 491 1 477 0.0 951
Msa0184440 MtrunA17_Chr1g0165681 76.356 461 107 2 33 491 1 461 0.0 732
Msa0184440 MtrunA17_Chr1g0165661 74.403 461 113 3 33 491 1 458 0.0 699
Msa0184440 MtrunA17_Chr1g0165711 73.913 460 76 4 33 491 1 417 0.0 674
Msa0184440 MtrunA17_Chr5g0433641 59.130 460 178 6 37 491 6 460 0.0 557
Msa0184440 MtrunA17_Chr7g0273781 56.394 477 192 8 11 478 12 481 0.0 553
Msa0184440 MtrunA17_Chr7g0273791 59.202 451 175 6 37 482 6 452 0.0 553
Msa0184440 MtrunA17_Chr7g0273721 58.478 460 182 5 37 491 7 462 0.0 550
Msa0184440 MtrunA17_Chr7g0273761 55.918 490 197 10 14 491 351 833 0.0 545
Msa0184440 MtrunA17_Chr7g0273761 55.449 312 127 6 178 482 32 338 1.85e-108 341
Msa0184440 MtrunA17_Chr7g0273731 55.021 478 199 7 6 470 50 524 0.0 537
Msa0184440 MtrunA17_Chr1g0191081 60.502 438 168 5 36 470 13 448 0.0 534
Msa0184440 MtrunA17_Chr6g0471381 69.811 265 78 2 229 491 6 270 6.89e-133 385
Msa0184440 MtrunA17_Chr8g0353421 91.473 129 11 0 33 161 1 129 1.30e-79 244
Msa0184440 MtrunA17_Chr1g0165741 78.912 147 31 0 33 179 1 147 2.02e-79 244
Msa0184440 MtrunA17_Chr3g0132001 33.178 428 267 9 41 462 14 428 3.43e-78 251
Msa0184440 MtrunA17_Chr8g0386141 32.739 449 284 9 33 476 1 436 1.19e-77 249
Msa0184440 MtrunA17_Chr6g0471371 76.000 150 36 0 33 182 1 150 1.34e-77 240
Msa0184440 MtrunA17_Chr6g0471971 51.477 237 86 6 193 423 1 214 9.82e-77 239
Msa0184440 MtrunA17_Chr7g0224391 34.031 382 240 7 46 421 21 396 2.13e-73 238
Msa0184440 MtrunA17_Chr6g0471891 60.914 197 51 4 262 456 1 173 1.59e-71 227
Msa0184440 MtrunA17_Chr7g0273701 57.303 178 70 4 316 491 1 174 3.54e-63 203
Msa0184440 MtrunA17_Chr7g0273741 58.108 148 62 0 32 179 1 148 3.74e-59 192
Msa0184440 MtrunA17_Chr2g0306321 31.973 441 278 14 49 470 26 463 7.28e-59 201
Msa0184440 MtrunA17_Chr4g0027071 29.801 453 293 10 35 467 6 453 1.28e-57 197
Msa0184440 MtrunA17_Chr3g0141851 33.676 389 234 10 51 426 22 399 6.44e-56 193
Msa0184440 MtrunA17_Chr4g0001861 31.481 432 264 16 48 462 10 426 8.94e-53 184
Msa0184440 MtrunA17_Chr3g0098961 56.376 149 65 0 31 179 3 151 1.33e-52 176
Msa0184440 MtrunA17_Chr1g0165721 75.214 117 29 0 375 491 1 117 1.71e-52 173
Msa0184440 MtrunA17_Chr2g0334001 29.742 427 275 11 49 462 11 425 7.08e-51 179
Msa0184440 MtrunA17_Chr1g0165731 70.370 108 31 1 193 299 1 108 1.41e-50 168
Msa0184440 MtrunA17_Chr3g0113821 30.485 433 273 11 49 466 13 432 1.05e-48 173
Msa0184440 MtrunA17_Chr8g0371821 29.724 434 274 12 47 466 9 425 1.84e-48 172
Msa0184440 MtrunA17_Chr6g0470591 29.340 409 253 13 88 469 63 462 2.03e-41 154
Msa0184440 MtrunA17_Chr2g0283361 28.986 414 281 10 62 466 25 434 2.96e-41 153
Msa0184440 MtrunA17_Chr7g0253501 28.632 475 263 17 41 464 2 451 1.62e-40 151
Msa0184440 MtrunA17_Chr7g0252731 28.218 404 244 13 33 421 11 383 3.01e-39 147
Msa0184440 MtrunA17_Chr7g0253531 27.719 469 275 16 41 464 2 451 1.26e-38 146
Msa0184440 MtrunA17_Chr7g0270771 29.293 297 173 7 37 322 1 271 6.39e-38 139
Msa0184440 MtrunA17_Chr4g0072041 27.617 449 280 14 49 466 22 456 1.12e-37 144
Msa0184440 MtrunA17_Chr7g0253541 27.069 447 268 18 49 454 12 441 1.81e-36 140
Msa0184440 MtrunA17_Chr4g0054081 28.172 465 290 16 33 466 1 452 5.77e-36 139
Msa0184440 MtrunA17_Chr3g0087401 26.034 411 264 10 45 422 6 409 1.31e-35 137
Msa0184440 MtrunA17_Chr3g0106691 27.511 458 280 15 48 469 63 504 2.44e-33 132
Msa0184440 MtrunA17_Chr7g0235531 26.471 442 275 14 51 464 14 433 2.00e-32 128
Msa0184440 MtrunA17_Chr3g0106661 27.731 476 262 17 48 469 17 464 3.31e-32 128
Msa0184440 MtrunA17_Chr2g0311401 27.815 302 197 8 174 462 2 295 4.42e-29 116
Msa0184440 MtrunA17_Chr3g0106721 27.566 341 219 8 48 368 17 349 1.80e-28 117
Msa0184440 MtrunA17_Chr3g0106761 26.634 413 261 14 86 469 4 403 3.43e-28 116
Msa0184440 MtrunA17_Chr3g0106741 27.763 389 239 13 110 469 59 434 1.38e-27 114
Msa0184440 MtrunA17_Chr4g0053081 25.862 406 257 15 78 460 49 433 2.16e-27 114
Msa0184440 MtrunA17_Chr7g0273691 54.945 91 37 2 197 284 1 90 1.52e-26 103
Msa0184440 MtrunA17_Chr4g0053061 24.532 481 316 18 5 460 7 465 1.56e-26 112
Msa0184440 MtrunA17_Chr3g0106711 34.211 190 115 4 48 229 17 204 2.80e-26 107
Msa0184440 MtrunA17_Chr3g0134071 28.211 436 256 19 38 431 4 424 1.64e-25 109
Msa0184440 MtrunA17_Chr4g0053111 26.106 452 278 18 40 460 9 435 2.37e-25 108
Msa0184440 MtrunA17_Chr5g0442701 26.065 399 235 14 93 457 63 435 4.59e-25 107
Msa0184440 MtrunA17_Chr4g0010121 26.633 398 240 14 55 427 70 440 5.29e-25 107
Msa0184440 MtrunA17_Chr4g0010361 26.606 436 259 20 55 460 25 429 5.59e-25 107
Msa0184440 MtrunA17_Chr3g0134091 29.154 319 201 12 55 359 30 337 7.73e-25 107
Msa0184440 MtrunA17_Chr5g0442691 25.490 408 226 14 93 457 86 458 7.84e-25 107
Msa0184440 MtrunA17_Chr8g0359251 26.773 437 252 19 55 460 30 429 1.71e-24 105
Msa0184440 MtrunA17_Chr5g0442721 26.250 400 234 15 93 457 64 437 2.39e-24 105
Msa0184440 MtrunA17_Chr8g0374911 24.887 442 260 18 56 460 52 458 3.50e-24 105
Msa0184440 MtrunA17_Chr4g0053091 24.651 430 261 14 55 460 32 422 6.60e-24 103
Msa0184440 MtrunA17_Chr7g0224131 27.570 428 261 19 55 460 25 425 7.81e-24 103
Msa0184440 MtrunA17_Chr3g0134041 27.295 403 224 17 68 431 44 416 2.94e-23 102
Msa0184440 MtrunA17_Chr3g0125001 27.378 431 267 18 55 467 25 427 4.88e-23 101
Msa0184440 MtrunA17_Chr5g0438701 23.724 392 256 14 47 418 9 377 5.23e-23 101
Msa0184440 MtrunA17_Chr8g0354371 24.654 434 281 15 46 460 22 428 5.84e-23 101
Msa0184440 MtrunA17_Chr8g0374641 26.984 441 249 21 55 460 30 432 6.40e-23 101
Msa0184440 MtrunA17_Chr3g0134061 26.368 402 243 16 68 431 42 428 1.37e-22 100
Msa0184440 MtrunA17_Chr3g0134121 26.067 445 276 19 56 473 31 449 1.56e-22 100
Msa0184440 MtrunA17_Chr5g0438681 24.427 393 251 14 47 418 9 376 2.05e-22 99.8
Msa0184440 MtrunA17_Chr3g0095721 25.432 405 217 14 96 455 72 436 2.59e-22 99.8
Msa0184440 MtrunA17_Chr4g0010331 25.472 424 272 15 55 460 25 422 2.82e-22 99.4
Msa0184440 MtrunA17_Chr4g0010311 25.349 430 271 18 55 460 25 428 4.45e-22 98.6
Msa0184440 MtrunA17_Chr3g0134111 25.738 474 262 20 49 475 21 451 1.34e-20 94.4
Msa0184440 MtrunA17_Chr8g0376891 22.993 461 297 15 47 480 9 438 4.59e-20 92.8
Msa0184440 MtrunA17_Chr4g0010351 28.344 314 196 10 55 353 25 324 1.06e-19 90.9
Msa0184440 MtrunA17_Chr5g0408911 25.564 399 251 15 56 427 35 414 3.28e-19 90.1
Msa0184440 MtrunA17_Chr8g0376901 25.191 393 255 16 47 418 9 383 1.04e-18 88.6
Msa0184440 MtrunA17_Chr7g0273681 65.306 49 17 0 123 171 1 49 1.36e-17 76.6
Msa0184440 MtrunA17_Chr4g0004691 28.221 163 111 2 51 213 14 170 1.72e-17 80.5
Msa0184440 MtrunA17_Chr8g0376911 22.983 409 243 16 47 418 9 382 9.33e-17 82.4
Msa0184440 MtrunA17_Chr7g0252771 27.490 251 166 6 221 462 4 247 1.07e-16 80.1
Msa0184440 MtrunA17_Chr6g0471981 57.812 64 25 1 132 195 1 62 8.38e-16 72.0
Msa0184440 MtrunA17_Chr2g0311411 35.766 137 82 4 49 184 11 142 1.01e-14 71.6
Msa0184440 MtrunA17_Chr5g0414431 62.264 53 15 1 86 138 5 52 1.17e-14 68.2
Msa0184440 MtrunA17_Chr7g0252761 29.677 155 105 3 37 190 12 163 6.44e-14 69.7
Msa0184440 MtrunA17_Chr4g0004701 27.273 198 116 8 285 462 45 234 2.60e-12 66.6
Msa0184440 MtrunA17_Chr1g0169471 35.052 97 55 2 119 214 11 100 4.34e-12 63.2
Msa0184440 MtrunA17_Chr5g0438721 32.000 125 68 5 96 214 9 122 1.01e-11 62.8
Msa0184440 MtrunA17_Chr3g0097841 28.205 156 94 4 281 420 47 200 2.32e-11 64.3
Msa0184440 MtrunA17_Chr7g0273671 66.667 42 14 0 32 73 1 42 2.88e-11 58.5
Msa0184440 MtrunA17_Chr3g0134131 28.395 162 112 1 260 417 122 283 4.18e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184440 AT5G17540.1 59.243 449 171 9 33 474 1 444 0.0 526
Msa0184440 AT3G03480.1 57.562 443 173 11 37 471 15 450 1.84e-169 486
Msa0184440 AT5G41040.2 32.207 444 279 10 32 466 8 438 5.80e-81 258
Msa0184440 AT5G41040.1 32.207 444 279 10 32 466 24 454 1.06e-80 258
Msa0184440 AT5G63560.1 33.409 443 269 11 33 467 1 425 5.40e-79 253
Msa0184440 AT3G48720.1 31.350 437 280 11 33 462 1 424 6.88e-74 240
Msa0184440 AT1G27620.1 30.201 447 281 12 42 476 10 437 2.25e-56 194
Msa0184440 AT3G62160.1 30.197 457 273 16 37 476 1 428 1.60e-54 189
Msa0184440 AT1G65450.1 29.322 457 287 13 32 470 11 449 3.39e-51 181
Msa0184440 AT2G40230.1 33.333 387 234 11 49 420 13 390 3.75e-50 177
Msa0184440 AT1G03390.1 30.193 467 298 16 14 466 3 455 4.52e-50 177
Msa0184440 AT1G28680.1 29.025 441 290 14 48 471 11 445 1.48e-48 173
Msa0184440 AT2G19070.1 29.070 430 264 13 67 466 29 447 5.52e-48 172
Msa0184440 AT5G57840.1 28.409 440 273 11 49 464 11 432 9.14e-48 171
Msa0184440 AT5G48930.1 29.680 438 270 14 49 466 11 430 3.51e-47 169
Msa0184440 AT1G28680.2 31.395 344 222 10 48 381 11 350 1.81e-44 161
Msa0184440 AT3G47170.1 27.310 487 277 17 35 470 6 466 3.68e-43 159
Msa0184440 AT5G07080.1 28.723 470 271 16 33 467 6 446 3.36e-42 156
Msa0184440 AT2G23510.1 28.541 466 284 17 33 469 5 450 2.12e-39 148
Msa0184440 AT2G25150.1 28.758 459 290 16 37 471 9 454 9.48e-39 147
Msa0184440 AT1G78990.1 27.820 399 250 12 94 471 65 446 3.22e-32 128
Msa0184440 AT1G31490.1 26.076 395 246 13 49 419 14 386 1.00e-29 121
Msa0184440 AT5G07860.1 26.846 447 272 15 47 467 24 441 1.71e-27 115
Msa0184440 AT1G65450.3 27.946 297 185 8 190 470 2 285 7.57e-27 110
Msa0184440 AT5G07870.1 26.872 454 263 17 47 460 24 448 5.16e-26 110
Msa0184440 AT1G32910.1 26.420 405 255 15 94 471 63 451 1.21e-25 109
Msa0184440 AT5G42830.1 27.692 455 247 17 47 460 23 436 1.95e-25 108
Msa0184440 AT1G24430.1 24.570 407 255 15 75 462 41 414 2.03e-24 105
Msa0184440 AT3G50270.1 27.119 413 233 15 55 429 30 412 3.17e-24 105
Msa0184440 AT5G07850.1 26.456 412 262 15 47 431 24 421 1.08e-23 103
Msa0184440 AT4G31910.1 27.051 451 276 15 49 462 17 451 3.15e-23 102
Msa0184440 AT3G50280.1 26.382 398 252 17 55 429 28 407 3.83e-23 102
Msa0184440 AT5G38130.1 25.879 398 252 14 55 429 48 425 5.45e-23 102
Msa0184440 AT5G67150.1 25.629 437 265 18 55 462 30 435 8.23e-23 101
Msa0184440 AT3G26040.1 23.485 396 260 14 47 420 9 383 1.99e-22 100
Msa0184440 AT3G50300.1 26.870 361 225 13 96 429 59 407 1.98e-21 97.1
Msa0184440 AT1G24420.2 26.434 401 235 17 47 418 11 380 1.66e-20 94.4
Msa0184440 AT1G24420.1 26.434 401 235 17 47 418 11 380 1.66e-20 94.4
Msa0184440 AT5G16410.1 25.862 348 223 12 94 420 89 422 1.61e-19 91.7
Msa0184440 AT5G47980.1 26.180 466 277 22 47 479 11 442 2.66e-19 90.9
Msa0184440 AT5G67160.1 26.406 409 238 15 90 467 50 426 3.42e-19 90.5
Msa0184440 AT3G30280.1 24.222 450 283 18 38 459 3 422 7.29e-17 83.2
Msa0184440 AT5G23940.1 24.823 423 238 16 93 470 62 449 8.42e-16 80.1
Msa0184440 AT3G50280.2 26.007 273 184 10 170 429 10 277 1.38e-15 78.2
Msa0184440 AT3G50290.1 25.556 270 160 9 170 414 16 269 9.03e-14 72.0
Msa0184440 AT5G47950.1 22.955 440 284 18 38 459 3 405 1.90e-13 72.8
Msa0184440 AT3G23840.1 28.643 199 117 7 112 301 81 263 5.50e-12 68.2
Msa0184440 AT4G15400.1 25.060 415 244 19 96 480 58 435 1.85e-11 66.6

Find 134 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCCATTGTTGGTGATTC+AGG 0.100165 1_4:-74007111 Msa0184440:intron
TTCAAACCAACATTAGTTTA+AGG 0.181902 1_4:+74004485 None:intergenic
AATTATACCTTTCCTCATTA+TGG 0.183641 1_4:-74007546 Msa0184440:CDS
TGCGGTGAGGATTTCGAAAT+TGG 0.188633 1_4:+74005097 None:intergenic
GGGTCTTTCCCTGCCATATT+TGG 0.199762 1_4:+74007371 None:intergenic
GCATTCAGTTGCGGATTTAA+TGG 0.222413 1_4:-74004838 Msa0184440:CDS
TTTGAGGGTCTTACAAATTC+AGG 0.246276 1_4:+74007488 None:intergenic
TTTGAAAACGTAACCCTTCT+TGG 0.271683 1_4:+74007423 None:intergenic
GGTATTTAGCTCAATTAATA+AGG 0.282791 1_4:+74004382 None:intergenic
TTGATAGTGGTTATTATAGA+AGG 0.302952 1_4:+74007587 None:intergenic
TAAACGAACTTATGATAATA+AGG 0.304041 1_4:+74004182 None:intergenic
CACCTAAGGCACTCATGAAT+TGG 0.305227 1_4:+74005351 None:intergenic
TGAATTTCCTTTGCTATGAT+TGG 0.316077 1_4:-74004466 Msa0184440:three_prime_UTR
GCGATGGGCCATGTCATCTA+AGG 0.318314 1_4:+74005190 None:intergenic
ATCCATTTGCAGGTAGGTTA+AGG 0.320236 1_4:-74007301 Msa0184440:CDS
TAAGGGAATAATTGTTCCTT+TGG 0.330029 1_4:+74005208 None:intergenic
TTGGAGATGTTGACTTTGGT+TGG 0.341202 1_4:-74004746 Msa0184440:CDS
GGTTGGGGAAAAGCTGTATA+TGG 0.341822 1_4:-74004729 Msa0184440:CDS
CCCGTCGCGGTTACAACCGC+TGG 0.344616 1_4:-74004939 Msa0184440:CDS
GATGTTAGTTTGAAAGAATT+TGG 0.347283 1_4:-74007209 Msa0184440:CDS
GAAACAATGAAACTAGGTTT+TGG 0.349312 1_4:+74004519 None:intergenic
TTATAAGTATGATCCAAATA+TGG 0.357454 1_4:-74007384 Msa0184440:CDS
GATGTGACACGTGCTGGATT+TGG 0.363433 1_4:-74004765 Msa0184440:CDS
TGCTAAAGGAGAGGAAGGTT+TGG 0.383515 1_4:-74004640 Msa0184440:CDS
TGGAGATGTTGACTTTGGTT+GGG 0.387463 1_4:-74004745 Msa0184440:CDS
CTGCTGCGTCACACATTGTA+TGG 0.389180 1_4:+74005381 None:intergenic
AACATTAACGAACCTACTAT+TGG 0.395610 1_4:-74004546 Msa0184440:CDS
AAATCCTCACCGCATGCCTT+TGG 0.410833 1_4:-74005088 Msa0184440:CDS
ATAATTGTTCCTTTGGTGTC+GGG 0.411133 1_4:+74005215 None:intergenic
CGAACTTCTTCGTCAGTGTC+TGG 0.418327 1_4:+74005041 None:intergenic
AAACTAATGGAAAATCCGTT+AGG 0.419834 1_4:-74004915 Msa0184440:CDS
TCCATTTGCAGGTAGGTTAA+GGG 0.425790 1_4:-74007300 Msa0184440:CDS
TGCGGATTTAATGGTTATCA+AGG 0.430701 1_4:-74004829 Msa0184440:CDS
CCTTAGGTGAAATTTCTTGC+GGG 0.433387 1_4:-74005337 Msa0184440:CDS
GTCCAATTCATGAGTGCCTT+AGG 0.433988 1_4:-74005353 Msa0184440:CDS
TACAACCGCTGGGAAACTAA+TGG 0.437340 1_4:-74004928 Msa0184440:CDS
TCCCTTAACCTACCTGCAAA+TGG 0.437418 1_4:+74007299 None:intergenic
CCAGCGGTTGTAACCGCGAC+GGG 0.438069 1_4:+74004939 None:intergenic
AATGTGTGACGCAGCAGGTT+TGG 0.438562 1_4:-74005375 Msa0184440:CDS
TCACTGTGAATGATAGAGAT+TGG 0.444139 1_4:+74007522 None:intergenic
AGGTCCTGACAGGAAACTTA+TGG 0.445457 1_4:-74007276 Msa0184440:CDS
AGTGCTTAACTCTCCATTGT+TGG 0.450887 1_4:-74007120 Msa0184440:CDS
GAAAGGTATAATTGAAGAAA+GGG 0.453489 1_4:+74007556 None:intergenic
GCATTACCGTAGTAACCATT+TGG 0.454482 1_4:+74004972 None:intergenic
ATATAATGCGCCACATGCAT+TGG 0.460594 1_4:+74007630 None:intergenic
TACTTATAAAATTGTATCAC+TGG 0.463814 1_4:+74007398 None:intergenic
AAGGGTACTAAATTTGATAG+TGG 0.464191 1_4:+74007574 None:intergenic
CACTGTGAATGATAGAGATT+GGG 0.466767 1_4:+74007523 None:intergenic
TTGAGGGTCTTACAAATTCA+GGG 0.476311 1_4:+74007489 None:intergenic
GGAAAGGTATAATTGAAGAA+AGG 0.476453 1_4:+74007555 None:intergenic
CAATCTCACCGGTATGGCAT+AGG 0.484304 1_4:-74005301 Msa0184440:CDS
TGTAGACCTTAAACTAATGT+TGG 0.490789 1_4:-74004491 Msa0184440:exon
TATAATGCGCCACATGCATT+GGG 0.492129 1_4:+74007631 None:intergenic
TTTAATCCACCGTTACCAAA+TGG 0.496653 1_4:-74004987 Msa0184440:CDS
GGATTTGGAGATGTTGACTT+TGG 0.497797 1_4:-74004750 Msa0184440:CDS
AAGTATGATCCAAATATGGC+AGG 0.501582 1_4:-74007380 Msa0184440:CDS
GTGTCTGATGTGACACGTGC+TGG 0.502256 1_4:-74004771 Msa0184440:CDS
TGAGTACATGTTACTCTTGG+TGG 0.504345 1_4:+74005257 None:intergenic
CGAATTGTGGCTACCTCGGT+TGG 0.505273 1_4:+74005152 None:intergenic
AGCTCATCCAAACAAGGAAA+AGG 0.507667 1_4:+74007170 None:intergenic
CGATGAGTACATGTTACTCT+TGG 0.511451 1_4:+74005254 None:intergenic
AACTTCTCCATGGCTTGAGC+TGG 0.517646 1_4:+74004600 None:intergenic
AATAATTGTTCCTTTGGTGT+CGG 0.522248 1_4:+74005214 None:intergenic
TGCCATACCGGTGAGATTGA+AGG 0.523692 1_4:+74005305 None:intergenic
AACTAATGGAAAATCCGTTA+GGG 0.528322 1_4:-74004914 Msa0184440:CDS
GGAGATGTTGACTTTGGTTG+GGG 0.528669 1_4:-74004744 Msa0184440:CDS
AACGATAAGACATTATATGA+TGG 0.532826 1_4:+74004272 None:intergenic
GGAGGAGTTGGTGCTATACC+TGG 0.535378 1_4:-74004693 Msa0184440:CDS
CACACATTGTATGGTTTAGA+CGG 0.536195 1_4:+74005390 None:intergenic
CATGTATTCTTGAGTTACAT+CGG 0.537828 1_4:+74004860 None:intergenic
GCACCAACTCCTCCTTTAGC+TGG 0.539177 1_4:+74004702 None:intergenic
GTATATGGTGGACCAGCTAA+AGG 0.539427 1_4:-74004714 Msa0184440:CDS
GCCTTAGGTGAAATTTCTTG+CGG 0.540414 1_4:-74005338 Msa0184440:CDS
ATTATTGCAGCAGCACTATG+TGG 0.541966 1_4:-74007659 Msa0184440:five_prime_UTR
CTCTTTGACGAACTTCTCCA+TGG 0.543009 1_4:+74004590 None:intergenic
AATCTCACCGGTATGGCATA+GGG 0.546127 1_4:-74005300 Msa0184440:CDS
TCCACCATAAGTTTCCTGTC+AGG 0.550793 1_4:+74007272 None:intergenic
GGTTAAGGGAAGGTCCTGAC+AGG 0.552476 1_4:-74007286 Msa0184440:CDS
TTGTTTACCAGCTCAAGCCA+TGG 0.552757 1_4:-74004607 Msa0184440:CDS
AGAAGCTCCCTATGCCATAC+CGG 0.552944 1_4:+74005293 None:intergenic
GGACCAGCTAAAGGAGGAGT+TGG 0.553044 1_4:-74004705 Msa0184440:CDS
ATAGGGAGCTTCTAAATGCA+AGG 0.554177 1_4:-74005283 Msa0184440:CDS
CAGATATAGATGACCAAGAA+GGG 0.559168 1_4:-74007436 Msa0184440:CDS
ATAGAGATTGGGCCATAATG+AGG 0.559544 1_4:+74007534 None:intergenic
TTTATTATCCATTTGCAGGT+AGG 0.559740 1_4:-74007307 Msa0184440:CDS
AGTGGTTATTATAGAAGGTA+CGG 0.560178 1_4:+74007592 None:intergenic
ATTAACGAACCTACTATTGG+TGG 0.562257 1_4:-74004543 Msa0184440:CDS
TCAGATATAGATGACCAAGA+AGG 0.565827 1_4:-74007437 Msa0184440:CDS
AGCGCGAATTGTGGCTACCT+CGG 0.567132 1_4:+74005148 None:intergenic
AACCTTCAATCTCACCGGTA+TGG 0.568331 1_4:-74005307 Msa0184440:CDS
CCCGCAAGAAATTTCACCTA+AGG 0.570019 1_4:+74005337 None:intergenic
CTCTATCATTCACAGTGAAG+AGG 0.571132 1_4:-74007517 Msa0184440:CDS
CCGTCGCGGTTACAACCGCT+GGG 0.572108 1_4:-74004938 Msa0184440:CDS
AGTATGATCCAAATATGGCA+GGG 0.572294 1_4:-74007379 Msa0184440:CDS
AACTCTAGTGCATACCCTAA+CGG 0.573134 1_4:+74004900 None:intergenic
CCGTTACCAAATGGTTACTA+CGG 0.574521 1_4:-74004978 Msa0184440:CDS
AGAATACATGCATTCAGTTG+CGG 0.574989 1_4:-74004847 Msa0184440:CDS
AATTATTCCCTTAGATGACA+TGG 0.576689 1_4:-74005198 Msa0184440:CDS
CATACAATGTGTGACGCAGC+AGG 0.579280 1_4:-74005380 Msa0184440:CDS
CGATTGTACGACAACGCCAA+AGG 0.587072 1_4:+74005072 None:intergenic
AATACCTGAATCACCAACAA+TGG 0.589517 1_4:+74007107 None:intergenic
ACATTAGTTTAAGGTCTACA+AGG 0.590944 1_4:+74004494 None:intergenic
CGATGGGCCATGTCATCTAA+GGG 0.603002 1_4:+74005191 None:intergenic
AAGGAAGAAACAATGAAACT+AGG 0.603955 1_4:+74004513 None:intergenic
TTATTGCAGCAGCACTATGT+GGG 0.605382 1_4:-74007658 Msa0184440:five_prime_UTR
GCGGATTTAATGGTTATCAA+GGG 0.606067 1_4:-74004828 Msa0184440:CDS
ATCTGCATTGTCAATGCTCG+TGG 0.609350 1_4:-74005014 Msa0184440:CDS
CCGTAGTAACCATTTGGTAA+CGG 0.610295 1_4:+74004978 None:intergenic
ATATAAAAGCTAGCAACACC+AGG 0.611199 1_4:+74004675 None:intergenic
TCTCACACCAATCATAGCAA+AGG 0.613641 1_4:+74004459 None:intergenic
GAACGAACCTTCAATCTCAC+CGG 0.615082 1_4:-74005312 Msa0184440:CDS
TGGGAGAAGAGCGCGAATTG+TGG 0.616656 1_4:+74005139 None:intergenic
CTTCTAACCACAGTAAAATG+TGG 0.618528 1_4:+74004801 None:intergenic
AAAAGTAGCTCATCCAAACA+AGG 0.619216 1_4:+74007164 None:intergenic
TGAGGGTCTTACAAATTCAG+GGG 0.622901 1_4:+74007490 None:intergenic
CCCAGCGGTTGTAACCGCGA+CGG 0.625128 1_4:+74004938 None:intergenic
ATGGTGGATTGTAATGATGA+AGG 0.626655 1_4:-74007257 Msa0184440:CDS
GATTGGGCCATAATGAGGAA+AGG 0.628876 1_4:+74007539 None:intergenic
GAACAAGTTCCCGACACCAA+AGG 0.634936 1_4:-74005224 Msa0184440:CDS
GCTTGAGCTGGTAAACAAAG+TGG 0.635861 1_4:+74004612 None:intergenic
TCATCCAAACAAGGAAAAGG+AGG 0.640809 1_4:+74007173 None:intergenic
TAGGGAGCTTCTAAATGCAA+GGG 0.644953 1_4:-74005282 Msa0184440:CDS
TTTGCAGGTAGGTTAAGGGA+AGG 0.651163 1_4:-74007296 Msa0184440:CDS
TAGTAACCATTTGGTAACGG+TGG 0.653193 1_4:+74004981 None:intergenic
TAGGTAACACGTCTCAAGTG+TGG 0.654765 1_4:-74005431 Msa0184440:CDS
AACGCCAAAGGCATGCGGTG+AGG 0.656317 1_4:+74005084 None:intergenic
TCCTGACAGGAAACTTATGG+TGG 0.657173 1_4:-74007273 Msa0184440:CDS
TGGGGAAAAGCTGTATATGG+TGG 0.663720 1_4:-74004726 Msa0184440:CDS
TATGGTGGACCAGCTAAAGG+AGG 0.665823 1_4:-74004711 Msa0184440:CDS
TTAAGCACTTCTGAAGAACC+AGG 0.667440 1_4:+74007134 None:intergenic
GTGGGTTATCCCAATGCATG+TGG 0.683744 1_4:-74007640 Msa0184440:exon
GTTGCAAAAGATAATCACGA+TGG 0.685988 1_4:-74004423 Msa0184440:three_prime_UTR
GTAACACGTCTCAAGTGTGG+TGG 0.692143 1_4:-74005428 Msa0184440:CDS
ACGACAACGCCAAAGGCATG+CGG 0.713965 1_4:+74005079 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTCTTTTGAAAAATTAA+CGG + chr1_4:74004928-74004947 None:intergenic 10.0%
!! AAATAGATAACTTTATATTA+TGG - chr1_4:74004943-74004962 Msa0184440:CDS 10.0%
!!! TATTTCTTTTGAAAAATTAA+CGG + chr1_4:74004928-74004947 None:intergenic 10.0%
!! AAATAGATAACTTTATATTA+TGG - chr1_4:74004943-74004962 Msa0184440:CDS 10.0%
!!! ATTATTTTAGGTTATTATTT+TGG + chr1_4:74005520-74005539 None:intergenic 10.0%
!!! TAACCTAAAATAATTTTTTA+AGG - chr1_4:74005526-74005545 Msa0184440:intron 10.0%
!!! TTTCCTTAAAAAATTATTTT+AGG + chr1_4:74005532-74005551 None:intergenic 10.0%
!!! ATTATCATTTTTCATTTTTT+TGG - chr1_4:74005705-74005724 Msa0184440:intron 10.0%
!!! TTATCATTTTTCATTTTTTT+GGG - chr1_4:74005706-74005725 Msa0184440:intron 10.0%
!! TATATTTCATAAATTTACAG+TGG - chr1_4:74004795-74004814 Msa0184440:CDS 15.0%
!!! ATTTTGGACTTTTTTTTTTT+TGG - chr1_4:74005298-74005317 Msa0184440:CDS 15.0%
!!! TTTTATGCAATTAAATAAGA+TGG + chr1_4:74005631-74005650 None:intergenic 15.0%
!! TATTTAATTGCATAAAAAAC+TGG - chr1_4:74005634-74005653 Msa0184440:intron 15.0%
!!! TATCATTTTTCATTTTTTTG+GGG - chr1_4:74005707-74005726 Msa0184440:intron 15.0%
!! TCAAAAAAAAAAAAAAAGAG+TGG - chr1_4:74006050-74006069 Msa0184440:intron 15.0%
!! CAAAAAAAAAAAAAAAGAGT+GGG - chr1_4:74006051-74006070 Msa0184440:intron 15.0%
!! TACTTATAAAATTGTATCAC+TGG + chr1_4:74004439-74004458 None:intergenic 20.0%
!! TTATAAGTATGATCCAAATA+TGG - chr1_4:74004450-74004469 Msa0184440:three_prime_UTR 20.0%
!!! CTTTTCTAATAATATCAACA+GGG + chr1_4:74004486-74004505 None:intergenic 20.0%
!! TGAAACAATATTTAAATCCT+AGG - chr1_4:74005444-74005463 Msa0184440:intron 20.0%
!!! ATCATTTTTCATTTTTTTGG+GGG - chr1_4:74005708-74005727 Msa0184440:intron 20.0%
!! CTTACATGTAAATTTGTAAA+TGG - chr1_4:74005734-74005753 Msa0184440:intron 20.0%
!!! TGGCACATATATTTTTTTAA+AGG - chr1_4:74005754-74005773 Msa0184440:intron 20.0%
!!! CAAGCACAAATAATTTTTTT+GGG - chr1_4:74005849-74005868 Msa0184440:intron 20.0%
!! AAAAAAATTTAATTCAGCTG+AGG - chr1_4:74005973-74005992 Msa0184440:intron 20.0%
!! TAGACAAAATTTATACATCA+CGG - chr1_4:74006223-74006242 Msa0184440:intron 20.0%
!!! AACTTTAACAACATTTTTCA+CGG + chr1_4:74007477-74007496 None:intergenic 20.0%
!! TAAACGAACTTATGATAATA+AGG + chr1_4:74007655-74007674 None:intergenic 20.0%
!!! TTGATAGTGGTTATTATAGA+AGG + chr1_4:74004250-74004269 None:intergenic 25.0%
!! GAAAGGTATAATTGAAGAAA+GGG + chr1_4:74004281-74004300 None:intergenic 25.0%
! AATTATACCTTTCCTCATTA+TGG - chr1_4:74004288-74004307 Msa0184440:three_prime_UTR 25.0%
!! GCTTTTCTAATAATATCAAC+AGG + chr1_4:74004487-74004506 None:intergenic 25.0%
!! GATGTTAGTTTGAAAGAATT+TGG - chr1_4:74004625-74004644 Msa0184440:CDS 25.0%
! ATATAGTACAAGTGTATGTA+CGG + chr1_4:74004852-74004871 None:intergenic 25.0%
! ATATAGTACGAGTATATGTT+GGG + chr1_4:74004898-74004917 None:intergenic 25.0%
! AATATAGTACGAGTATATGT+TGG + chr1_4:74004899-74004918 None:intergenic 25.0%
! CATATACTCGTACTATATTA+AGG - chr1_4:74004900-74004919 Msa0184440:CDS 25.0%
!!! AACTTAAGTTGGTTAGATTT+TGG - chr1_4:74005282-74005301 Msa0184440:CDS 25.0%
!!! GACTTTTTTTTTTTTGGTAG+TGG - chr1_4:74005304-74005323 Msa0184440:CDS 25.0%
! TTTCTATCGAATAGAATCTT+AGG - chr1_4:74005491-74005510 Msa0184440:intron 25.0%
! ATGCAATTAAATAAGATGGT+AGG + chr1_4:74005627-74005646 None:intergenic 25.0%
!! GTTCACAAACAAAATTTTAC+AGG - chr1_4:74005826-74005845 Msa0184440:intron 25.0%
!!! GCAAGCACAAATAATTTTTT+TGG - chr1_4:74005848-74005867 Msa0184440:intron 25.0%
! AAAAAAAAAAAAAGAGTGGG+TGG - chr1_4:74006054-74006073 Msa0184440:intron 25.0%
! AAAAAAAAAAAAGAGTGGGT+GGG - chr1_4:74006055-74006074 Msa0184440:intron 25.0%
! ATACCTTTCAAAAATGCTAA+AGG - chr1_4:74007180-74007199 Msa0184440:CDS 25.0%
!! TTCAAACCAACATTAGTTTA+AGG + chr1_4:74007352-74007371 None:intergenic 25.0%
!! GGTATTTAGCTCAATTAATA+AGG + chr1_4:74007455-74007474 None:intergenic 25.0%
!! ACTTTAACAACATTTTTCAC+GGG + chr1_4:74007476-74007495 None:intergenic 25.0%
! AACGATAAGACATTATATGA+TGG + chr1_4:74007565-74007584 None:intergenic 25.0%
AGTGGTTATTATAGAAGGTA+CGG + chr1_4:74004245-74004264 None:intergenic 30.0%
!! AAGGGTACTAAATTTGATAG+TGG + chr1_4:74004263-74004282 None:intergenic 30.0%
! GGAAAGGTATAATTGAAGAA+AGG + chr1_4:74004282-74004301 None:intergenic 30.0%
! GAGAGTAATTTTACTTCATG+AGG + chr1_4:74004379-74004398 None:intergenic 30.0%
!! GTGTTTTATTATCCATTTGC+AGG - chr1_4:74004523-74004542 Msa0184440:CDS 30.0%
TTTATTATCCATTTGCAGGT+AGG - chr1_4:74004527-74004546 Msa0184440:CDS 30.0%
AACTTAGACACAACTTAAGT+TGG - chr1_4:74005271-74005290 Msa0184440:CDS 30.0%
!!! TTTTTTTTTTTGGTAGTGGC+TGG - chr1_4:74005308-74005327 Msa0184440:CDS 30.0%
!!! TTTTTTTTTTGGTAGTGGCT+GGG - chr1_4:74005309-74005328 Msa0184440:CDS 30.0%
! ACACTTAGATTTTGGACTAA+TGG - chr1_4:74005396-74005415 Msa0184440:CDS 30.0%
! TTGATTGAAACGACACATTT+TGG + chr1_4:74006082-74006101 None:intergenic 30.0%
!! CTTGAATTTTGACCATTTGT+AGG - chr1_4:74006384-74006403 Msa0184440:intron 30.0%
! AATAATTGTTCCTTTGGTGT+CGG + chr1_4:74006623-74006642 None:intergenic 30.0%
TAAGGGAATAATTGTTCCTT+TGG + chr1_4:74006629-74006648 None:intergenic 30.0%
AATTATTCCCTTAGATGACA+TGG - chr1_4:74006636-74006655 Msa0184440:intron 30.0%
!!! CGAAATTGGATTGTTTTTGT+TGG + chr1_4:74006726-74006745 None:intergenic 30.0%
AAACTAATGGAAAATCCGTT+AGG - chr1_4:74006919-74006938 Msa0184440:intron 30.0%
AACTAATGGAAAATCCGTTA+GGG - chr1_4:74006920-74006939 Msa0184440:intron 30.0%
CATGTATTCTTGAGTTACAT+CGG + chr1_4:74006977-74006996 None:intergenic 30.0%
! TCTCCTTTAGCATTTTTGAA+AGG + chr1_4:74007186-74007205 None:intergenic 30.0%
TTTCAAAAATGCTAAAGGAG+AGG - chr1_4:74007185-74007204 Msa0184440:CDS 30.0%
AACATTAACGAACCTACTAT+TGG - chr1_4:74007288-74007307 Msa0184440:CDS 30.0%
! GAAACAATGAAACTAGGTTT+TGG + chr1_4:74007318-74007337 None:intergenic 30.0%
AAGGAAGAAACAATGAAACT+AGG + chr1_4:74007324-74007343 None:intergenic 30.0%
! ACATTAGTTTAAGGTCTACA+AGG + chr1_4:74007343-74007362 None:intergenic 30.0%
TGTAGACCTTAAACTAATGT+TGG - chr1_4:74007343-74007362 Msa0184440:CDS 30.0%
! TGAATTTCCTTTGCTATGAT+TGG - chr1_4:74007368-74007387 Msa0184440:CDS 30.0%
CACTGTGAATGATAGAGATT+GGG + chr1_4:74004314-74004333 None:intergenic 35.0%
TCACTGTGAATGATAGAGAT+TGG + chr1_4:74004315-74004334 None:intergenic 35.0%
TTGAGGGTCTTACAAATTCA+GGG + chr1_4:74004348-74004367 None:intergenic 35.0%
TTTGAGGGTCTTACAAATTC+AGG + chr1_4:74004349-74004368 None:intergenic 35.0%
!!! TCATGAGGTGTTTGTTTTGA+GGG + chr1_4:74004364-74004383 None:intergenic 35.0%
!!! TTCATGAGGTGTTTGTTTTG+AGG + chr1_4:74004365-74004384 None:intergenic 35.0%
TCAGATATAGATGACCAAGA+AGG - chr1_4:74004397-74004416 Msa0184440:three_prime_UTR 35.0%
CAGATATAGATGACCAAGAA+GGG - chr1_4:74004398-74004417 Msa0184440:three_prime_UTR 35.0%
TTTGAAAACGTAACCCTTCT+TGG + chr1_4:74004414-74004433 None:intergenic 35.0%
AAGTATGATCCAAATATGGC+AGG - chr1_4:74004454-74004473 Msa0184440:three_prime_UTR 35.0%
AGTATGATCCAAATATGGCA+GGG - chr1_4:74004455-74004474 Msa0184440:three_prime_UTR 35.0%
!! ATGGTGGATTGTAATGATGA+AGG - chr1_4:74004577-74004596 Msa0184440:CDS 35.0%
!!! TGAAGGTGTTTTGTTCATTG+AGG - chr1_4:74004594-74004613 Msa0184440:CDS 35.0%
AAAAGTAGCTCATCCAAACA+AGG + chr1_4:74004673-74004692 None:intergenic 35.0%
AATACCTGAATCACCAACAA+TGG + chr1_4:74004730-74004749 None:intergenic 35.0%
!!! TTTTTTTTTGGTAGTGGCTG+GGG - chr1_4:74005310-74005329 Msa0184440:CDS 35.0%
GACAATGCATAAAGTATGCA+AGG + chr1_4:74005349-74005368 None:intergenic 35.0%
! TGCAAGACACTTTTATTCCT+AGG + chr1_4:74005464-74005483 None:intergenic 35.0%
AAATGATTCAAGTGAGGTCA+AGG + chr1_4:74005921-74005940 None:intergenic 35.0%
CTACACAAATGATTCAAGTG+AGG + chr1_4:74005927-74005946 None:intergenic 35.0%
CTCACTTGAATCATTTGTGT+AGG - chr1_4:74005925-74005944 Msa0184440:intron 35.0%
! TCTTTGTTTCCTAGAAAGAG+TGG - chr1_4:74006021-74006040 Msa0184440:intron 35.0%
! CTTTGTTTCCTAGAAAGAGT+GGG - chr1_4:74006022-74006041 Msa0184440:intron 35.0%
TTTGTTTCCTAGAAAGAGTG+GGG - chr1_4:74006023-74006042 Msa0184440:intron 35.0%
AGACACACACTAGAGTTAAA+AGG + chr1_4:74006121-74006140 None:intergenic 35.0%
CACAACAAGTGTTTCATTTG+TGG - chr1_4:74006308-74006327 Msa0184440:intron 35.0%
CACACATTGTATGGTTTAGA+CGG + chr1_4:74006447-74006466 None:intergenic 35.0%
!! ATAATTGTTCCTTTGGTGTC+GGG + chr1_4:74006622-74006641 None:intergenic 35.0%
!!! ATTGTTTTTGTTGGTGAGGT+GGG + chr1_4:74006717-74006736 None:intergenic 35.0%
!!! TTGGATTGTTTTTGTTGGTG+AGG + chr1_4:74006721-74006740 None:intergenic 35.0%
TTTAATCCACCGTTACCAAA+TGG - chr1_4:74006847-74006866 Msa0184440:intron 35.0%
!! ATTTTCCATTAGTTTCCCAG+CGG + chr1_4:74006914-74006933 None:intergenic 35.0%
AGAATACATGCATTCAGTTG+CGG - chr1_4:74006987-74007006 Msa0184440:intron 35.0%
TGCGGATTTAATGGTTATCA+AGG - chr1_4:74007005-74007024 Msa0184440:intron 35.0%
GCGGATTTAATGGTTATCAA+GGG - chr1_4:74007006-74007025 Msa0184440:intron 35.0%
CTTCTAACCACAGTAAAATG+TGG + chr1_4:74007036-74007055 None:intergenic 35.0%
ATATAAAAGCTAGCAACACC+AGG + chr1_4:74007162-74007181 None:intergenic 35.0%
AAAAATGCTAAAGGAGAGGA+AGG - chr1_4:74007189-74007208 Msa0184440:CDS 35.0%
ATTAACGAACCTACTATTGG+TGG - chr1_4:74007291-74007310 Msa0184440:CDS 35.0%
GTTGCAAAAGATAATCACGA+TGG - chr1_4:74007411-74007430 Msa0184440:CDS 35.0%
TATAATGCGCCACATGCATT+GGG + chr1_4:74004206-74004225 None:intergenic 40.0%
ATATAATGCGCCACATGCAT+TGG + chr1_4:74004207-74004226 None:intergenic 40.0%
ATAGAGATTGGGCCATAATG+AGG + chr1_4:74004303-74004322 None:intergenic 40.0%
CTCTATCATTCACAGTGAAG+AGG - chr1_4:74004317-74004336 Msa0184440:three_prime_UTR 40.0%
TGAGGGTCTTACAAATTCAG+GGG + chr1_4:74004347-74004366 None:intergenic 40.0%
ATCCATTTGCAGGTAGGTTA+AGG - chr1_4:74004533-74004552 Msa0184440:CDS 40.0%
TCCATTTGCAGGTAGGTTAA+GGG - chr1_4:74004534-74004553 Msa0184440:CDS 40.0%
! TCAACCTCCTTTTCCTTGTT+TGG - chr1_4:74004657-74004676 Msa0184440:CDS 40.0%
TCATCCAAACAAGGAAAAGG+AGG + chr1_4:74004664-74004683 None:intergenic 40.0%
AGCTCATCCAAACAAGGAAA+AGG + chr1_4:74004667-74004686 None:intergenic 40.0%
!!! GAGCTACTTTTTGATGTTCC+TGG - chr1_4:74004682-74004701 Msa0184440:CDS 40.0%
! TTAAGCACTTCTGAAGAACC+AGG + chr1_4:74004703-74004722 None:intergenic 40.0%
AGTGCTTAACTCTCCATTGT+TGG - chr1_4:74004714-74004733 Msa0184440:CDS 40.0%
CAAGTGTATGTACGGATTTG+CGG + chr1_4:74004844-74004863 None:intergenic 40.0%
! TGAGTTTAGCTCAGTTGGTT+GGG + chr1_4:74005371-74005390 None:intergenic 40.0%
! TCACGAGGACACTTAGATTT+TGG - chr1_4:74005388-74005407 Msa0184440:CDS 40.0%
TCACGGATCAAGCTATTACA+TGG - chr1_4:74006240-74006259 Msa0184440:intron 40.0%
GGATCAAGCTATTACATGGT+TGG - chr1_4:74006244-74006263 Msa0184440:intron 40.0%
TGAGACGTGTTACCTACAAA+TGG + chr1_4:74006399-74006418 None:intergenic 40.0%
GCCTTAGGTGAAATTTCTTG+CGG - chr1_4:74006496-74006515 Msa0184440:intron 40.0%
CCTTAGGTGAAATTTCTTGC+GGG - chr1_4:74006497-74006516 Msa0184440:intron 40.0%
ATAGGGAGCTTCTAAATGCA+AGG - chr1_4:74006551-74006570 Msa0184440:intron 40.0%
TAGGGAGCTTCTAAATGCAA+GGG - chr1_4:74006552-74006571 Msa0184440:intron 40.0%
TGAGTACATGTTACTCTTGG+TGG + chr1_4:74006580-74006599 None:intergenic 40.0%
CGATGAGTACATGTTACTCT+TGG + chr1_4:74006583-74006602 None:intergenic 40.0%
ACCGAAGAAAAAAGAGCGAT+GGG + chr1_4:74006662-74006681 None:intergenic 40.0%
!!! GATTGTTTTTGTTGGTGAGG+TGG + chr1_4:74006718-74006737 None:intergenic 40.0%
!! TAGTAACCATTTGGTAACGG+TGG + chr1_4:74006856-74006875 None:intergenic 40.0%
CCGTAGTAACCATTTGGTAA+CGG + chr1_4:74006859-74006878 None:intergenic 40.0%
CCGTTACCAAATGGTTACTA+CGG - chr1_4:74006856-74006875 Msa0184440:intron 40.0%
GCATTACCGTAGTAACCATT+TGG + chr1_4:74006865-74006884 None:intergenic 40.0%
AACTCTAGTGCATACCCTAA+CGG + chr1_4:74006937-74006956 None:intergenic 40.0%
GCATTCAGTTGCGGATTTAA+TGG - chr1_4:74006996-74007015 Msa0184440:intron 40.0%
!! GGATTTGGAGATGTTGACTT+TGG - chr1_4:74007084-74007103 Msa0184440:intron 40.0%
!! TTGGAGATGTTGACTTTGGT+TGG - chr1_4:74007088-74007107 Msa0184440:intron 40.0%
!! TGGAGATGTTGACTTTGGTT+GGG - chr1_4:74007089-74007108 Msa0184440:intron 40.0%
TCTCACACCAATCATAGCAA+AGG + chr1_4:74007378-74007397 None:intergenic 40.0%
GATTGGGCCATAATGAGGAA+AGG + chr1_4:74004298-74004317 None:intergenic 45.0%
TCCCTTAACCTACCTGCAAA+TGG + chr1_4:74004538-74004557 None:intergenic 45.0%
TTTGCAGGTAGGTTAAGGGA+AGG - chr1_4:74004538-74004557 Msa0184440:CDS 45.0%
AGGTCCTGACAGGAAACTTA+TGG - chr1_4:74004558-74004577 Msa0184440:CDS 45.0%
TCCTGACAGGAAACTTATGG+TGG - chr1_4:74004561-74004580 Msa0184440:CDS 45.0%
TCCACCATAAGTTTCCTGTC+AGG + chr1_4:74004565-74004584 None:intergenic 45.0%
!! CTCTCCATTGTTGGTGATTC+AGG - chr1_4:74004723-74004742 Msa0184440:CDS 45.0%
CGAGTATATGTTGGGATGAG+TGG + chr1_4:74004890-74004909 None:intergenic 45.0%
CATAAAGTATGCAAGGTCCG+AGG + chr1_4:74005342-74005361 None:intergenic 45.0%
! GTGAGTTTAGCTCAGTTGGT+TGG + chr1_4:74005372-74005391 None:intergenic 45.0%
TGACAAACCCCACTCTTTCT+AGG + chr1_4:74006033-74006052 None:intergenic 45.0%
TAGGTAACACGTCTCAAGTG+TGG - chr1_4:74006403-74006422 Msa0184440:intron 45.0%
GTCCAATTCATGAGTGCCTT+AGG - chr1_4:74006481-74006500 Msa0184440:intron 45.0%
! CACCTAAGGCACTCATGAAT+TGG + chr1_4:74006486-74006505 None:intergenic 45.0%
CCCGCAAGAAATTTCACCTA+AGG + chr1_4:74006500-74006519 None:intergenic 45.0%
GAACGAACCTTCAATCTCAC+CGG - chr1_4:74006522-74006541 Msa0184440:intron 45.0%
AACCTTCAATCTCACCGGTA+TGG - chr1_4:74006527-74006546 Msa0184440:intron 45.0%
AATCTCACCGGTATGGCATA+GGG - chr1_4:74006534-74006553 Msa0184440:intron 45.0%
!!! GCCCATCGCTCTTTTTTCTT+CGG - chr1_4:74006658-74006677 Msa0184440:intron 45.0%
GACCGAAGAAAAAAGAGCGA+TGG + chr1_4:74006663-74006682 None:intergenic 45.0%
!!! TTTTTTCTTCGGTCCAACCG+AGG - chr1_4:74006669-74006688 Msa0184440:intron 45.0%
TGCGGTGAGGATTTCGAAAT+TGG + chr1_4:74006740-74006759 None:intergenic 45.0%
ATCTGCATTGTCAATGCTCG+TGG - chr1_4:74006820-74006839 Msa0184440:intron 45.0%
TACAACCGCTGGGAAACTAA+TGG - chr1_4:74006906-74006925 Msa0184440:intron 45.0%
! GGGTAGACCACATTTTACTG+TGG - chr1_4:74007026-74007045 Msa0184440:intron 45.0%
!! GGAGATGTTGACTTTGGTTG+GGG - chr1_4:74007090-74007109 Msa0184440:intron 45.0%
GGTTGGGGAAAAGCTGTATA+TGG - chr1_4:74007105-74007124 Msa0184440:intron 45.0%
TGGGGAAAAGCTGTATATGG+TGG - chr1_4:74007108-74007127 Msa0184440:intron 45.0%
!! GTATATGGTGGACCAGCTAA+AGG - chr1_4:74007120-74007139 Msa0184440:CDS 45.0%
TGCTAAAGGAGAGGAAGGTT+TGG - chr1_4:74007194-74007213 Msa0184440:CDS 45.0%
GCTTGAGCTGGTAAACAAAG+TGG + chr1_4:74007225-74007244 None:intergenic 45.0%
TTGTTTACCAGCTCAAGCCA+TGG - chr1_4:74007227-74007246 Msa0184440:CDS 45.0%
CTCTTTGACGAACTTCTCCA+TGG + chr1_4:74007247-74007266 None:intergenic 45.0%
!!! GGTTTTGGACCACCAATAGT+AGG + chr1_4:74007303-74007322 None:intergenic 45.0%
!! ATAAATTAATTAGTTAAAAT+TGG + chr1_4:74006335-74006354 None:intergenic 5.0%
! GTGGGTTATCCCAATGCATG+TGG - chr1_4:74004194-74004213 Msa0184440:three_prime_UTR 50.0%
GGGTCTTTCCCTGCCATATT+TGG + chr1_4:74004466-74004485 None:intergenic 50.0%
!!! TTTTGGTAGTGGCTGGGGTT+TGG - chr1_4:74005315-74005334 Msa0184440:CDS 50.0%
! CCTCGTGAGTTTAGCTCAGT+TGG + chr1_4:74005376-74005395 None:intergenic 50.0%
CCAACTGAGCTAAACTCACG+AGG - chr1_4:74005373-74005392 Msa0184440:CDS 50.0%
GTAACACGTCTCAAGTGTGG+TGG - chr1_4:74006406-74006425 Msa0184440:intron 50.0%
CTGCTGCGTCACACATTGTA+TGG + chr1_4:74006456-74006475 None:intergenic 50.0%
CATACAATGTGTGACGCAGC+AGG - chr1_4:74006454-74006473 Msa0184440:intron 50.0%
AATGTGTGACGCAGCAGGTT+TGG - chr1_4:74006459-74006478 Msa0184440:intron 50.0%
TGCCATACCGGTGAGATTGA+AGG + chr1_4:74006532-74006551 None:intergenic 50.0%
CAATCTCACCGGTATGGCAT+AGG - chr1_4:74006533-74006552 Msa0184440:intron 50.0%
AGAAGCTCCCTATGCCATAC+CGG + chr1_4:74006544-74006563 None:intergenic 50.0%
GAACAAGTTCCCGACACCAA+AGG - chr1_4:74006610-74006629 Msa0184440:intron 50.0%
CGATGGGCCATGTCATCTAA+GGG + chr1_4:74006646-74006665 None:intergenic 50.0%
AAATCCTCACCGCATGCCTT+TGG - chr1_4:74006746-74006765 Msa0184440:intron 50.0%
CGATTGTACGACAACGCCAA+AGG + chr1_4:74006765-74006784 None:intergenic 50.0%
CGAACTTCTTCGTCAGTGTC+TGG + chr1_4:74006796-74006815 None:intergenic 50.0%
GATGTGACACGTGCTGGATT+TGG - chr1_4:74007069-74007088 Msa0184440:intron 50.0%
!! TATGGTGGACCAGCTAAAGG+AGG - chr1_4:74007123-74007142 Msa0184440:CDS 50.0%
AACTTCTCCATGGCTTGAGC+TGG + chr1_4:74007237-74007256 None:intergenic 50.0%
GGTTAAGGGAAGGTCCTGAC+AGG - chr1_4:74004548-74004567 Msa0184440:CDS 55.0%
GCGATGGGCCATGTCATCTA+AGG + chr1_4:74006647-74006666 None:intergenic 55.0%
CGAATTGTGGCTACCTCGGT+TGG + chr1_4:74006685-74006704 None:intergenic 55.0%
AGCGCGAATTGTGGCTACCT+CGG + chr1_4:74006689-74006708 None:intergenic 55.0%
! TGGGAGAAGAGCGCGAATTG+TGG + chr1_4:74006698-74006717 None:intergenic 55.0%
! ACGACAACGCCAAAGGCATG+CGG + chr1_4:74006758-74006777 None:intergenic 55.0%
! TGCTTTTGCGTTTCCCGTCG+CGG - chr1_4:74006882-74006901 Msa0184440:intron 55.0%
GTGTCTGATGTGACACGTGC+TGG - chr1_4:74007063-74007082 Msa0184440:intron 55.0%
GGACCAGCTAAAGGAGGAGT+TGG - chr1_4:74007129-74007148 Msa0184440:CDS 55.0%
GCACCAACTCCTCCTTTAGC+TGG + chr1_4:74007135-74007154 None:intergenic 55.0%
!! GGAGGAGTTGGTGCTATACC+TGG - chr1_4:74007141-74007160 Msa0184440:CDS 55.0%
AGTGGCTGGGGTTTGGACCT+CGG - chr1_4:74005322-74005341 Msa0184440:CDS 60.0%
! AACGCCAAAGGCATGCGGTG+AGG + chr1_4:74006753-74006772 None:intergenic 60.0%
CCAGCGGTTGTAACCGCGAC+GGG + chr1_4:74006898-74006917 None:intergenic 65.0%
CCCAGCGGTTGTAACCGCGA+CGG + chr1_4:74006899-74006918 None:intergenic 65.0%
CCGTCGCGGTTACAACCGCT+GGG - chr1_4:74006896-74006915 Msa0184440:intron 65.0%
CCCGTCGCGGTTACAACCGC+TGG - chr1_4:74006895-74006914 Msa0184440:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74004178 74007678 74004178 ID=Msa0184440;Name=Msa0184440
chr1_4 mRNA 74004178 74007678 74004178 ID=Msa0184440-mRNA-1;Parent=Msa0184440;Name=Msa0184440-mRNA-1;_AED=0.14;_eAED=0.14;_QI=33|1|1|1|1|1|2|331|491
chr1_4 exon 74004178 74005450 74004178 ID=Msa0184440-mRNA-1:exon:25207;Parent=Msa0184440-mRNA-1
chr1_4 exon 74007112 74007678 74007112 ID=Msa0184440-mRNA-1:exon:25206;Parent=Msa0184440-mRNA-1
chr1_4 five_prime_UTR 74007646 74007678 74007646 ID=Msa0184440-mRNA-1:five_prime_utr;Parent=Msa0184440-mRNA-1
chr1_4 CDS 74007112 74007645 74007112 ID=Msa0184440-mRNA-1:cds;Parent=Msa0184440-mRNA-1
chr1_4 CDS 74004509 74005450 74004509 ID=Msa0184440-mRNA-1:cds;Parent=Msa0184440-mRNA-1
chr1_4 three_prime_UTR 74004178 74004508 74004178 ID=Msa0184440-mRNA-1:three_prime_utr;Parent=Msa0184440-mRNA-1
Gene Sequence

>Msa0184440

ATGTGGCGCATTATATATACACTTGCATATACCGTACCTTCTATAATAACCACTATCAAATTTAGTACCCTTTCTTCAATTATACCTTTCCTCATTATGGCCCAATCTCTATCATTCACAGTGAAGAGGCATGCCCCTGAATTTGTAAGACCCTCAAAACAAACACCTCATGAAGTAAAATTACTCTCAGATATAGATGACCAAGAAGGGTTACGTTTTCAAATTCCAGTGATACAATTTTATAAGTATGATCCAAATATGGCAGGGAAAGACCCTGTTGATATTATTAGAAAAGCATTAGCAAAAACACTTGTGTTTTATTATCCATTTGCAGGTAGGTTAAGGGAAGGTCCTGACAGGAAACTTATGGTGGATTGTAATGATGAAGGTGTTTTGTTCATTGAGGCTGATGCTGATGTTAGTTTGAAAGAATTTGGTGATGCTCTTCAACCTCCTTTTCCTTGTTTGGATGAGCTACTTTTTGATGTTCCTGGTTCTTCAGAAGTGCTTAACTCTCCATTGTTGGTGATTCAGGTAACACGTCTCAAGTGTGGTGGTTTCATTTTTGCTCTCCGTCTAAACCATACAATGTGTGACGCAGCAGGTTTGGTCCAATTCATGAGTGCCTTAGGTGAAATTTCTTGCGGGATGAACGAACCTTCAATCTCACCGGTATGGCATAGGGAGCTTCTAAATGCAAGGGATCCACCAAGAGTAACATGTACTCATCGTGAATATGAACAAGTTCCCGACACCAAAGGAACAATTATTCCCTTAGATGACATGGCCCATCGCTCTTTTTTCTTCGGTCCAACCGAGGTAGCCACAATTCGCGCTCTTCTCCCACCTCACCAACAAAAACAATCCAATTTCGAAATCCTCACCGCATGCCTTTGGCGTTGTCGTACAATCGCATTACAACCAGACACTGACGAAGAAGTTCGCATAATCTGCATTGTCAATGCTCGTGGCAAGTTTAATCCACCGTTACCAAATGGTTACTACGGTAATGCTTTTGCGTTTCCCGTCGCGGTTACAACCGCTGGGAAACTAATGGAAAATCCGTTAGGGTATGCACTAGAGTTAGTTAAAAAAGCAAAATCCGATGTAACTCAAGAATACATGCATTCAGTTGCGGATTTAATGGTTATCAAGGGTAGACCACATTTTACTGTGGTTAGAAGTTATCTAGTGTCTGATGTGACACGTGCTGGATTTGGAGATGTTGACTTTGGTTGGGGAAAAGCTGTATATGGTGGACCAGCTAAAGGAGGAGTTGGTGCTATACCTGGTGTTGCTAGCTTTTATATACCTTTCAAAAATGCTAAAGGAGAGGAAGGTTTGGTTATACCACTTTGTTTACCAGCTCAAGCCATGGAGAAGTTCGTCAAAGAGTTAGATAGTGTACTTAAGAACAACATTAACGAACCTACTATTGGTGGTCCAAAACCTAGTTTCATTGTTTCTTCCTTGTAG
Protein sequence

>Msa0184440

MWRIIYTLAYTVPSIITTIKFSTLSSIIPFLIMAQSLSFTVKRHAPEFVRPSKQTPHEVKLLSDIDDQEGLRFQIPVIQFYKYDPNMAGKDPVDIIRKALAKTLVFYYPFAGRLREGPDRKLMVDCNDEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNSPLLVIQVTRLKCGGFIFALRLNHTMCDAAGLVQFMSALGEISCGMNEPSISPVWHRELLNARDPPRVTCTHREYEQVPDTKGTIIPLDDMAHRSFFFGPTEVATIRALLPPHQQKQSNFEILTACLWRCRTIALQPDTDEEVRIICIVNARGKFNPPLPNGYYGNAFAFPVAVTTAGKLMENPLGYALELVKKAKSDVTQEYMHSVADLMVIKGRPHFTVVRSYLVSDVTRAGFGDVDFGWGKAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKELDSVLKNNINEPTIGGPKPSFIVSSL*