AlfalfaGEDB | Msa0150560

M. sativa cultivar zhongmu-4 / Msa0150560

KEGG: K14497
GO: GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010029 GO:0010030 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:0140096 GO:1900140 GO:1901419 GO:1901420 GO:1901564 GO:1905957 GO:1905958 GO:2000026
PFAM: PF00481.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0150560	sp\|Q5SN75\|P2C08_ORYSJ	55.519	308	101	7	105	379	89	393	4.66e-101	308

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0150560	A0A072VG64	93.438	381	24	1	1	380	6	386	0.0	677

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0104540	Msa0150560	0.875200	3.717562e-68	-8.615850e-47
Msa0150560	Msa0222340	0.818420	1.978852e-52	-8.615850e-47
Msa0150560	Msa0343400	0.874871	4.811447e-68	-8.615850e-47
Msa0150560	Msa0358730	0.802209	6.254793e-49	-8.615850e-47
Msa0150560	Msa0878880	0.811896	5.567388e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0150560	MtrunA17_Chr1g0158781	93.438	381	24	1	1	380	6	386	0.0	677
Msa0150560	MtrunA17_Chr3g0112291	50.955	314	120	10	84	374	74	376	5.35e-90	276
Msa0150560	MtrunA17_Chr5g0396731	53.684	285	117	5	106	377	72	354	1.08e-89	274
Msa0150560	MtrunA17_Chr4g0070871	47.401	327	133	8	87	376	182	506	2.49e-83	263
Msa0150560	MtrunA17_Chr1g0150781	50.000	306	112	7	108	374	241	544	3.51e-82	261
Msa0150560	MtrunA17_Chr3g0135961	48.232	311	117	6	108	375	232	541	3.05e-81	258
Msa0150560	MtrunA17_Chr5g0435721	52.536	276	103	5	107	360	193	462	6.36e-80	253
Msa0150560	MtrunA17_Chr2g0299981	45.000	320	127	7	104	375	219	537	3.35e-78	250
Msa0150560	MtrunA17_Chr8g0389921	46.599	294	128	5	105	374	147	435	2.43e-72	232
Msa0150560	MtrunA17_Chr1g0150451	38.754	289	146	8	86	371	61	321	1.77e-46	162

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0150560	AT2G29380.1	48.795	332	141	8	61	374	41	361	2.14e-90	276
Msa0150560	AT1G07430.1	46.023	352	134	8	72	374	90	434	3.77e-88	273
Msa0150560	AT3G11410.1	54.878	246	85	3	108	330	106	348	4.68e-84	261
Msa0150560	AT5G59220.1	55.738	244	85	4	107	332	112	350	4.59e-82	257
Msa0150560	AT4G26080.1	49.164	299	122	7	104	374	127	423	9.13e-81	254
Msa0150560	AT1G72770.4	47.649	319	110	8	108	375	191	503	4.02e-80	254
Msa0150560	AT1G72770.1	47.649	319	110	8	108	375	191	503	4.02e-80	254
Msa0150560	AT1G72770.3	47.649	319	110	8	108	375	191	503	4.02e-80	254
Msa0150560	AT5G57050.2	48.355	304	124	7	104	375	71	373	1.69e-78	246
Msa0150560	AT5G57050.1	48.355	304	124	7	104	375	111	413	2.13e-78	248

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Find 105 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGATAGGGAGGTGGC+GGG	0.596854	1_4:+12177583	Msa0150560:CDS
AAACATGAAACGACACTCAT+CGG	0.550810	1_4:+12176945	Msa0150560:CDS
AAATTAGGAGAGTTTGTCAT+GGG	0.571829	1_4:+12180173	Msa0150560:CDS
AACATTCTCTAGCTTCGTAA+CGG	0.604268	1_4:-12177004	None:intergenic
AATGCTTCAAAGGTCAAATT+AGG	0.290408	1_4:+12180158	Msa0150560:CDS
AATGGAGGATGCAGTGAGTG+TGG	0.528815	1_4:+12177375	Msa0150560:CDS
AATGTTAACTTGTGCTCTTA+AGG	0.364179	1_4:+12180354	Msa0150560:three_prime_UTR
ACATAGGATTGTGGCGGAGG+AGG	0.510112	1_4:+12177498	Msa0150560:CDS
ACCGGAGAAGATTGAAAATT+CGG	0.361358	1_4:+12177028	Msa0150560:CDS
ACGACCTCTATCGCATGGTT+CGG	0.552860	1_4:+12177327	Msa0150560:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCTTAAATTCTCTCATAAAG+AGG	+	chr1_4:12178862-12178881	Msa0150560:intron	30.0%
GATCATATACACAAAACATG+TGG	-	chr1_4:12178930-12178949	None:intergenic	30.0%
AATCCCACATAATCAAAGTT+TGG	+	chr1_4:12179182-12179201	Msa0150560:intron	30.0%
ATCCCACATAATCAAAGTTT+GGG	+	chr1_4:12179183-12179202	Msa0150560:intron	30.0%
TGATCAAGTATTTAACGAGT+CGG	-	chr1_4:12179412-12179431	None:intergenic	30.0%
TCGAGTTTAGTTAAAAACTC+AGG	-	chr1_4:12179648-12179667	None:intergenic	30.0%
GTTGTAAGAAAATGCTTCAA+AGG	+	chr1_4:12180148-12180167	Msa0150560:CDS	30.0%
AATGCTTCAAAGGTCAAATT+AGG	+	chr1_4:12180158-12180177	Msa0150560:CDS	30.0%
AAATTAGGAGAGTTTGTCAT+GGG	+	chr1_4:12180173-12180192	Msa0150560:CDS	30.0%
TAGATCTTAATGTTCCTCTT+AGG	-	chr1_4:12180300-12180319	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	12176940	12180373	12176940	ID=Msa0150560;Name=Msa0150560
chr1_4	mRNA	12176940	12180373	12176940	ID=Msa0150560-mRNA-1;Parent=Msa0150560;Name=Msa0150560-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|1\|0.5\|1\|1\|1\|4\|49\|380
chr1_4	exon	12176940	12177224	12176940	ID=Msa0150560-mRNA-1:exon:4946;Parent=Msa0150560-mRNA-1
chr1_4	exon	12177307	12177747	12177307	ID=Msa0150560-mRNA-1:exon:4947;Parent=Msa0150560-mRNA-1
chr1_4	exon	12178504	12178609	12178504	ID=Msa0150560-mRNA-1:exon:4948;Parent=Msa0150560-mRNA-1
chr1_4	exon	12180014	12180373	12180014	ID=Msa0150560-mRNA-1:exon:4949;Parent=Msa0150560-mRNA-1
chr1_4	CDS	12176940	12177224	12176940	ID=Msa0150560-mRNA-1:cds;Parent=Msa0150560-mRNA-1
chr1_4	CDS	12177307	12177747	12177307	ID=Msa0150560-mRNA-1:cds;Parent=Msa0150560-mRNA-1
chr1_4	CDS	12178504	12178609	12178504	ID=Msa0150560-mRNA-1:cds;Parent=Msa0150560-mRNA-1
chr1_4	CDS	12180014	12180324	12180014	ID=Msa0150560-mRNA-1:cds;Parent=Msa0150560-mRNA-1
chr1_4	three_prime_UTR	12180325	12180373	12180325	ID=Msa0150560-mRNA-1:three_prime_utr;Parent=Msa0150560-mRNA-1

Gene Sequence

>Msa0150560

ATGAAAAACATGAAACGACACTCATCGGAATCTTCTCTCCGACGAGTGGATTCTCCGGTTGGAGCCGTTACGAAGCTAGAGAATGTTCACCGGAGAAGATTGAAAATTCGGAGAATGAAGTATAGTTGTCAAGCAAAGATCCACGTCGGAAATGGCTCCGACGACGGTAGTTTAGAAACGGAGAATGCTCGACGAGAGATTCATGAATCGGTTGAGATATCGTTGTCGTTAGCAACTTCTTCATCATCGGAGGAAGATGATCAGCCGTCCAAGCAAAACGGAGAGGTGAAGCATTATCAACAACAACGACCTCTATCGCATGGTTCGGTGTCAACGATAGGGTGTAGGAAGGAAATGGAGGATGCAGTGAGTGTGGAGACAGATTTTACGATGAAAGATGGAGAGAAGTGTGATTTTTTCGGTGTTTATGACGGTCATGGTGGTGCTCAGGTGGCGGAGGCGTGTAGGGAGAGATTACATAGGATTGTGGCGGAGGAGGTGGAGAAGTATGGGAATGATGGGGAATTTGAGTGGGAGAGAGTGATGGAAGGGTGTTTTGGTAAAATGGATAGGGAGGTGGCGGGTGATGCCGCGGTTAGGACCGTGGGGTCTACTGCAGTTGTGGCGGTTGTTGTTAAGGAGGCGATCGTTGTTGCAAACTGTGGTGATTCTAGAGCCGTCTTGGGAAGAGGTGGTGAAGCTTTGGATTTGTCAAGTGATCATAAGCCAAACAGGCCTGATGAGTTGATGAGAATAGAAGAAGCTGGTGGAAGAGTTATAAACTGGAATGGCCACCGTGTTCTTGGTGTTCTTGCAACCTCACGATCTATAGGAGATCAATATCTTCGACCATATGTGATATCAAAACCTGAAGTCACTGTGACAAAGAGAACCAATGATGATGAATTTCTCATACTAGCAAGTGATGGCTTGTGGGGTGTAATGTCTAGTGAAATTGCATGCCAAGTTGTAAGAAAATGCTTCAAAGGTCAAATTAGGAGAGTTTGTCATGGGAATGAGAATCAAAGTAATCGTGCTGCTGAAGCTGCAACACTATTGTCTGAGATAGCATTGGCAAAGGGTAGCAGAGACAATACAAGTGTCATAGTTGTTGACCTAAGAGGAACATTAAGATCTAGTTGA

Protein sequence

>Msa0150560

MKNMKRHSSESSLRRVDSPVGAVTKLENVHRRRLKIRRMKYSCQAKIHVGNGSDDGSLETENARREIHESVEISLSLATSSSSEEDDQPSKQNGEVKHYQQQRPLSHGSVSTIGCRKEMEDAVSVETDFTMKDGEKCDFFGVYDGHGGAQVAEACRERLHRIVAEEVEKYGNDGEFEWERVMEGCFGKMDREVAGDAAVRTVGSTAVVAVVVKEAIVVANCGDSRAVLGRGGEALDLSSDHKPNRPDELMRIEEAGGRVINWNGHRVLGVLATSRSIGDQYLRPYVISKPEVTVTKRTNDDEFLILASDGLWGVMSSEIACQVVRKCFKGQIRRVCHGNENQSNRAAEAATLLSEIALAKGSRDNTSVIVVDLRGTLRSS*