AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0163970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163970 MtrunA17_Chr1g0179211 98.883 358 4 0 1 358 1 358 0.0 729
Msa0163970 MtrunA17_Chr7g0265821 87.151 358 46 0 1 358 1 358 0.0 654
Msa0163970 MtrunA17_Chr5g0429051 85.475 358 52 0 1 358 1 358 0.0 637
Msa0163970 MtrunA17_Chr5g0447091 76.536 358 83 1 1 358 1 357 0.0 560
Msa0163970 MtrunA17_Chr4g0028361 55.682 352 151 4 8 358 53 400 8.92e-132 382
Msa0163970 MtrunA17_Chr4g0035951 55.398 352 152 4 8 358 53 400 4.67e-131 380
Msa0163970 MtrunA17_Chr1g0208981 52.809 356 163 4 4 358 40 391 1.07e-128 374
Msa0163970 MtrunA17_Chr5g0429031 84.360 211 33 0 1 211 42 252 8.53e-126 361
Msa0163970 MtrunA17_Chr4g0035981 53.556 239 106 4 121 358 6 240 5.52e-82 249
Msa0163970 MtrunA17_Chr5g0429021 79.389 131 27 0 228 358 1 131 1.90e-73 224
Msa0163970 MtrunA17_Chr7g0265811 69.792 96 4 1 112 207 21 91 3.85e-37 129
Msa0163970 MtrunA17_Chr4g0035971 55.814 86 38 0 8 93 51 136 3.61e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163970 AT2G36460.1 87.151 358 46 0 1 358 1 358 0.0 647
Msa0163970 AT3G52930.1 86.592 358 48 0 1 358 1 358 0.0 640
Msa0163970 AT5G03690.2 86.072 359 49 1 1 358 1 359 0.0 614
Msa0163970 AT5G03690.1 86.533 349 46 1 11 358 45 393 0.0 599
Msa0163970 AT4G26530.1 77.933 358 79 0 1 358 1 358 0.0 574
Msa0163970 AT4G26530.3 77.933 358 79 0 1 358 1 358 0.0 574
Msa0163970 AT4G26530.2 77.933 358 79 0 1 358 1 358 0.0 574
Msa0163970 AT4G26520.1 73.184 358 96 0 1 358 1 358 0.0 534
Msa0163970 AT4G26520.3 73.315 356 95 0 1 356 1 356 0.0 530
Msa0163970 AT4G26520.2 73.066 349 94 0 10 358 7 355 0.0 519
Msa0163970 AT4G26520.4 73.066 349 94 0 10 358 7 355 0.0 519
Msa0163970 AT2G36460.3 88.015 267 32 0 92 358 1 267 6.26e-176 489
Msa0163970 AT2G36460.2 88.015 267 32 0 92 358 1 267 6.26e-176 489
Msa0163970 AT5G03690.3 87.266 267 33 1 93 358 1 267 5.62e-164 459
Msa0163970 AT5G03690.4 87.266 267 33 1 93 358 1 267 5.62e-164 459
Msa0163970 AT4G38970.1 55.367 354 153 4 6 358 49 398 1.45e-132 384
Msa0163970 AT2G21330.1 54.237 354 157 4 6 358 50 399 1.91e-129 377
Msa0163970 AT2G01140.1 55.114 352 153 4 8 358 44 391 4.91e-129 375
Msa0163970 AT2G21330.3 52.260 354 154 5 6 358 50 389 2.87e-120 353
Msa0163970 AT4G38970.2 55.212 259 114 2 6 263 49 306 1.63e-93 284
Msa0163970 AT2G21330.2 53.282 259 119 2 6 263 50 307 2.66e-91 276

Find 104 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTCTGATTGAACTTGTTT+TGG 0.229590 1_4:+42517490 Msa0163970:three_prime_UTR
TGGACTCTTACATTCTCTTA+TGG 0.280031 1_4:+42517261 Msa0163970:CDS
AGCAATGCAGCTTGAGCTTT+AGG 0.286122 1_4:-42517339 None:intergenic
CTCAAGCTGCATTGCTTGTT+AGG 0.288958 1_4:+42517346 Msa0163970:CDS
GATCCTGGTGTAACCATATT+AGG 0.309736 1_4:-42517069 None:intergenic
ACAAATACTAATATGTTAAG+AGG 0.319325 1_4:+42517460 Msa0163970:exon
GACAAGGGAGATGGTTCATT+TGG 0.333091 1_4:-42516844 None:intergenic
TCAAGCTGAACTGGCTTGAT+TGG 0.334716 1_4:+42517607 Msa0163970:three_prime_UTR
GCTGCTTACATTGGCTCTCC+TGG 0.358440 1_4:+42515813 Msa0163970:CDS
TAATGCAATGAACAAACTTA+AGG 0.360057 1_4:+42517227 Msa0163970:CDS
ATAACCTCAGGAGCAACTTT+AGG 0.366412 1_4:-42517099 None:intergenic
CCGGTGCAAGGTTCGCTAAA+TGG 0.381985 1_4:+42516800 Msa0163970:CDS
TTGTGGTAAAGTATCAATAA+TGG 0.382123 1_4:+42517582 Msa0163970:three_prime_UTR
AAGAAAGCTGGAGTTCTTCC+TGG 0.384415 1_4:+42516673 Msa0163970:CDS
TTCAGAGTCTCTTCATGTTA+AGG 0.387381 1_4:+42517434 Msa0163970:CDS
AGTTGTATGCATCATGGTTA+TGG 0.395562 1_4:+42517513 Msa0163970:three_prime_UTR
AATGCAATGAACAAACTTAA+GGG 0.427010 1_4:+42517228 Msa0163970:CDS
AGAATCTCAGGTTCTACAAT+TGG 0.430166 1_4:-42516931 None:intergenic
GACCATCATGTTCTTCTTGA+AGG 0.430660 1_4:+42517033 Msa0163970:CDS
ATAATGGCTTCAAGCTGAAC+TGG 0.434807 1_4:+42517598 Msa0163970:three_prime_UTR
CAAAGCTCGGACTGTGTGTT+GGG 0.435174 1_4:-42517124 None:intergenic
GTTTGTGCCGGCAAGTTCAA+TGG 0.437135 1_4:-42516716 None:intergenic
GCAGCAAGGATACCCTTTCC+AGG 0.444842 1_4:-42515831 None:intergenic
AACATTCTCCACATTGATGC+TGG 0.446255 1_4:-42515889 None:intergenic
CTTGAAAGAAGACATGTTAG+TGG 0.447312 1_4:-42514356 None:intergenic
AAGGGAACCATTGAACTTGC+CGG 0.451723 1_4:+42516709 Msa0163970:CDS
TGGAGTTCTTCCTGGTATCA+AGG 0.454086 1_4:+42516681 Msa0163970:CDS
ATTGAACTTGCCGGCACAAA+CGG 0.455923 1_4:+42516718 Msa0163970:CDS
TGTCGTTTCTCCGTTTGTGC+CGG 0.458489 1_4:-42516728 None:intergenic
CCCTTGTCAACCTTGATACC+AGG 0.464495 1_4:-42516691 None:intergenic
AGAAACGACAACTCAAGGTT+TGG 0.468747 1_4:+42516741 Msa0163970:CDS
AACTTAAGGGAAAGAAGCCT+TGG 0.474897 1_4:+42517241 Msa0163970:CDS
GATGAATCTACTGGCACAAT+CGG 0.481067 1_4:+42515855 Msa0163970:CDS
GCCGTCATATGTCAAGAGAA+TGG 0.487020 1_4:+42516901 Msa0163970:CDS
GATCCATCCACTAGAATCTC+AGG 0.492290 1_4:-42516943 None:intergenic
TACATTGGCTCTCCTGGAAA+GGG 0.492987 1_4:+42515819 Msa0163970:CDS
TGTTCTTGCAGCATGTTACA+AGG 0.495030 1_4:+42517002 Msa0163970:CDS
CTGAGGCAATCTAAACAACC+AGG 0.497144 1_4:-42515954 None:intergenic
ACTTCAGCAAAGTACTCTTA+AGG 0.501897 1_4:+42517290 Msa0163970:CDS
TTCTTCAACACATCCACAAA+GGG 0.502730 1_4:-42516655 None:intergenic
AGAGCCAATGTAAGCAGCAT+TGG 0.511633 1_4:-42515808 None:intergenic
GCAAAGTACTCTTAAGGCAT+GGG 0.512733 1_4:+42517296 Msa0163970:CDS
GTTGCGAACCGTTCCTGCCG+CGG 0.513144 1_4:+42517146 Msa0163970:CDS
GTGTGTTGGGCTATAACCTC+AGG 0.515555 1_4:-42517111 None:intergenic
CTTGCTGCTGATGAATCTAC+TGG 0.520412 1_4:+42515846 Msa0163970:CDS
TCGTGGATTGACAAGGGAGA+TGG 0.521733 1_4:-42516853 None:intergenic
ATTGCCAATGCTGCTTACAT+TGG 0.522063 1_4:+42515804 Msa0163970:CDS
ACAAAGCTCGGACTGTGTGT+TGG 0.522609 1_4:-42517125 None:intergenic
TTACATTGGCTCTCCTGGAA+AGG 0.522941 1_4:+42515818 Msa0163970:CDS
CTCTTACATTCTCTTATGGA+AGG 0.525023 1_4:+42517265 Msa0163970:CDS
GTGGATGTGTTGAAGAAAGC+TGG 0.525235 1_4:+42516661 Msa0163970:CDS
ACTATAGCAGGAACCGCGGC+AGG 0.528273 1_4:-42517159 None:intergenic
AGCAGGAACCGCGGCAGGAA+CGG 0.528619 1_4:-42517154 None:intergenic
AGCAAAGTACTCTTAAGGCA+TGG 0.530894 1_4:+42517295 Msa0163970:CDS
ACATGCTGCAAGAACACGTT+CGG 0.537011 1_4:-42516995 None:intergenic
TAAGAGAATGTAAGAGTCCA+AGG 0.537565 1_4:-42517258 None:intergenic
CTCTCCTAAAGTTGCTCCTG+AGG 0.537898 1_4:+42517095 Msa0163970:CDS
AGAAGACAAGTAGTAAGAAG+AGG 0.543018 1_4:-42514260 None:intergenic
GCAACTCTAGGAACTTATCA+AGG 0.543212 1_4:+42517387 Msa0163970:CDS
TCTTTCAAGAGCAAGTATGA+AGG 0.544555 1_4:+42514370 Msa0163970:CDS
GAACCTGAGATTCTAGTGGA+TGG 0.544943 1_4:+42516940 Msa0163970:CDS
ACCATTCTCTTGACATATGA+CGG 0.546843 1_4:-42516902 None:intergenic
TCAATCCACGAAAACGCCTA+CGG 0.549910 1_4:+42516865 Msa0163970:CDS
ACTTTAGGAGAGTCTGATCC+TGG 0.549951 1_4:-42517084 None:intergenic
CCAGACAAGAAAACTATAGC+AGG 0.555235 1_4:-42517171 None:intergenic
CCATTTAGCGAACCTTGCAC+CGG 0.555662 1_4:-42516800 None:intergenic
TGCAAGAAGTACTATGAAGC+CGG 0.557100 1_4:+42516781 Msa0163970:CDS
ACTTTCAGTACATAGAATGA+TGG 0.558514 1_4:-42517855 None:intergenic
AACGGAGAAACGACAACTCA+AGG 0.559975 1_4:+42516736 Msa0163970:CDS
GCAAACTCAGAAGCAACTCT+AGG 0.561535 1_4:+42517375 Msa0163970:CDS
GAACGGTTCGCAACAAAGCT+CGG 0.563519 1_4:-42517137 None:intergenic
GTGTATAGGAGTACCCTTTG+TGG 0.565160 1_4:+42516642 Msa0163970:intron
TATCAAGGTAATGCTAACCT+TGG 0.568421 1_4:+42517402 Msa0163970:CDS
TCCTGGTATCAAGGTTGACA+AGG 0.571342 1_4:+42516690 Msa0163970:CDS
CATATGTCAAGAGAATGGTT+TGG 0.575303 1_4:+42516906 Msa0163970:CDS
TGGCGTGCCGTGCTAAAGAT+CGG 0.577059 1_4:+42516820 Msa0163970:CDS
AGTACTATGAAGCCGGTGCA+AGG 0.593122 1_4:+42516788 Msa0163970:CDS
ACTCAAGGTTTGGATGACCT+TGG 0.593476 1_4:+42516751 Msa0163970:CDS
GGTTGTTTAGATTGCCTCAG+TGG 0.598140 1_4:+42515957 Msa0163970:CDS
ACTTCTTGCACCTCTGACCA+AGG 0.600417 1_4:-42516768 None:intergenic
GTTTGGATGACCTTGGTCAG+AGG 0.601427 1_4:+42516758 Msa0163970:CDS
CAAATACTAATATGTTAAGA+GGG 0.607367 1_4:+42517461 Msa0163970:exon
AGAATCTGTAATACCTGCAG+CGG 0.609033 1_4:-42516015 None:intergenic
AAGCCTAATATGGTTACACC+AGG 0.609198 1_4:+42517066 Msa0163970:CDS
TGTAGAACCTGAGATTCTAG+TGG 0.614806 1_4:+42516936 Msa0163970:CDS
AAAAGTGTAAAGCAGAAAGT+GGG 0.614949 1_4:+42517756 Msa0163970:three_prime_UTR
TCTTACATTCTCTTATGGAA+GGG 0.615325 1_4:+42517266 Msa0163970:CDS
ATTTGGACCGATCTTTAGCA+CGG 0.615328 1_4:-42516827 None:intergenic
CAGTAGATTCATCAGCAGCA+AGG 0.619396 1_4:-42515845 None:intergenic
TTTCTTCAACACATCCACAA+AGG 0.625245 1_4:-42516656 None:intergenic
GTCTGGTGGACAAAGTGAAG+AGG 0.625664 1_4:+42517188 Msa0163970:CDS
AGTACTCTTAAGGCATGGGG+TGG 0.629413 1_4:+42517300 Msa0163970:CDS
TACCTTCAAGAAGAACATGA+TGG 0.642921 1_4:-42517035 None:intergenic
CAAAGTACTCTTAAGGCATG+GGG 0.654273 1_4:+42517297 Msa0163970:CDS
GGTAATGCTAACCTTGGTGA+TGG 0.655737 1_4:+42517408 Msa0163970:CDS
AATATGTTAAGAGGGTTGTG+AGG 0.659689 1_4:+42517469 Msa0163970:exon
CCTGGTATCAAGGTTGACAA+GGG 0.660914 1_4:+42516691 Msa0163970:CDS
GCGATTTGCCAGCATCAATG+TGG 0.675073 1_4:+42515881 Msa0163970:CDS
TGAGGCAATCTAAACAACCA+GGG 0.678050 1_4:-42515953 None:intergenic
GAAAACTATAGCAGGAACCG+CGG 0.691811 1_4:-42517163 None:intergenic
GAGGCAATCTAAACAACCAG+GGG 0.706971 1_4:-42515952 None:intergenic
ACTCTGAAGCACCATCACCA+AGG 0.713624 1_4:-42517419 None:intergenic
GGCATAACGAGCTAAACCGT+AGG 0.727129 1_4:-42516881 None:intergenic
CGAACAAGATAACACCACTG+AGG 0.795503 1_4:-42515971 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTATTTTCAATACAT+AGG + chr1_4:42514438-42514457 Msa0163970:intron 10.0%
!! TTATTAAAAATTAGAAAGTA+AGG - chr1_4:42516467-42516486 None:intergenic 10.0%
!!! TATCAGATTAAATTTTCATA+TGG - chr1_4:42515435-42515454 None:intergenic 15.0%
!!! TTTTTATCTTTCATAGATAT+CGG + chr1_4:42516145-42516164 Msa0163970:intron 15.0%
!! TTAAATAAGTAAATCCAAAA+AGG - chr1_4:42516179-42516198 None:intergenic 15.0%
!! TAAAAAAAGCTAAATTCAAA+GGG - chr1_4:42517813-42517832 None:intergenic 15.0%
!! ATGAGAAAGTAAAAAACAAT+GGG - chr1_4:42514185-42514204 None:intergenic 20.0%
!! AATGAGAAAGTAAAAAACAA+TGG - chr1_4:42514186-42514205 None:intergenic 20.0%
!!! TGAGTTTTGAAGATTTATAA+AGG - chr1_4:42514236-42514255 None:intergenic 20.0%
!!! TTCTAAAAGTACTAAAAAAG+AGG + chr1_4:42514770-42514789 Msa0163970:intron 20.0%
!! AAAAAGACAAATTGTGTATA+AGG + chr1_4:42514794-42514813 Msa0163970:intron 20.0%
!! AAAAGACAAATTGTGTATAA+GGG + chr1_4:42514795-42514814 Msa0163970:intron 20.0%
!! AAAAAGTAAGAGTAAAATAG+AGG + chr1_4:42515106-42515125 Msa0163970:intron 20.0%
!! ACATTTGTTTGACAAAATTA+CGG + chr1_4:42515247-42515266 Msa0163970:intron 20.0%
!! AATTAAGATATTTGACTCTT+TGG - chr1_4:42515493-42515512 None:intergenic 20.0%
!!! AATGTTTATTTTGAATTAGC+AGG + chr1_4:42516092-42516111 Msa0163970:intron 20.0%
!! AATAAACATAAAACAGCTTA+AGG - chr1_4:42516261-42516280 None:intergenic 20.0%
!!! TTTTAATAAATTCTTTCGTG+CGG + chr1_4:42516478-42516497 Msa0163970:intron 20.0%
!! ACAAATACTAATATGTTAAG+AGG + chr1_4:42517460-42517479 Msa0163970:exon 20.0%
!! CAAATACTAATATGTTAAGA+GGG + chr1_4:42517461-42517480 Msa0163970:exon 20.0%
!!! AATAGTGTTTGTTTTTGTTT+AGG + chr1_4:42517555-42517574 Msa0163970:three_prime_UTR 20.0%
!! AAAAAAAGCTAAATTCAAAG+GGG - chr1_4:42517812-42517831 None:intergenic 20.0%
!! CTAAAAAAAGCTAAATTCAA+AGG - chr1_4:42517814-42517833 None:intergenic 20.0%
!!! CTTTGAATTTAGCTTTTTTT+AGG + chr1_4:42517812-42517831 Msa0163970:three_prime_UTR 20.0%
! TCATAATTGTGTGATTTGAT+TGG + chr1_4:42514660-42514679 Msa0163970:intron 25.0%
! CTCAAGTAAAATATGTATTG+AGG + chr1_4:42514706-42514725 Msa0163970:intron 25.0%
!!! TCGAAATTTTGATGTCAAAT+AGG + chr1_4:42514838-42514857 Msa0163970:intron 25.0%
!!! CGAAATTTTGATGTCAAATA+GGG + chr1_4:42514839-42514858 Msa0163970:intron 25.0%
! TTCTTATTGGTCAAAAACAA+TGG - chr1_4:42515020-42515039 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr1_4:42515033-42515052 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG + chr1_4:42515032-42515051 Msa0163970:intron 25.0%
! AACTTAGTGTATGTTTGAAT+TGG - chr1_4:42515355-42515374 None:intergenic 25.0%
! GTTCGATTCATAAAACTAAA+GGG + chr1_4:42515393-42515412 Msa0163970:intron 25.0%
! TGATAGTAGATTACAAACTA+TGG + chr1_4:42515450-42515469 Msa0163970:intron 25.0%
! AATCGTTGAAATCTAAATCT+AGG - chr1_4:42516126-42516145 None:intergenic 25.0%
! AAAATTAGAAAGTAAGGACA+TGG - chr1_4:42516461-42516480 None:intergenic 25.0%
! TAATGCAATGAACAAACTTA+AGG + chr1_4:42517227-42517246 Msa0163970:CDS 25.0%
! AATGCAATGAACAAACTTAA+GGG + chr1_4:42517228-42517247 Msa0163970:CDS 25.0%
!! TTGTGGTAAAGTATCAATAA+TGG + chr1_4:42517582-42517601 Msa0163970:three_prime_UTR 25.0%
!!! TTGGCATTTATTTTTCAACA+TGG + chr1_4:42517626-42517645 Msa0163970:three_prime_UTR 25.0%
!!! TTTTTTGTTACACACACTTT+TGG - chr1_4:42517740-42517759 None:intergenic 25.0%
! AAAAAAGCTAAATTCAAAGG+GGG - chr1_4:42517811-42517830 None:intergenic 25.0%
!!! GTTTTGTTTTGTGTGATGTT+TGG - chr1_4:42514293-42514312 None:intergenic 30.0%
! ATGTTACACTAGCTTCATTT+AGG + chr1_4:42514490-42514509 Msa0163970:intron 30.0%
ATGTATTGAGGTTTACGTTT+AGG + chr1_4:42514718-42514737 Msa0163970:intron 30.0%
AGGTTTACGTTTAGGTATAT+TGG + chr1_4:42514726-42514745 Msa0163970:intron 30.0%
GGTTTACGTTTAGGTATATT+GGG + chr1_4:42514727-42514746 Msa0163970:intron 30.0%
GTTTACGTTTAGGTATATTG+GGG + chr1_4:42514728-42514747 Msa0163970:intron 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + chr1_4:42514935-42514954 Msa0163970:intron 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr1_4:42515058-42515077 Msa0163970:intron 30.0%
!! ACTCGTTTTAAGTAAGATTC+CGG + chr1_4:42515144-42515163 Msa0163970:intron 30.0%
! ACTATTTACCACGGTAATTT+TGG - chr1_4:42515313-42515332 None:intergenic 30.0%
TCAAACATACACTAAGTTTG+TGG + chr1_4:42515357-42515376 Msa0163970:intron 30.0%
GGTTCGATTCATAAAACTAA+AGG + chr1_4:42515392-42515411 Msa0163970:intron 30.0%
AATTTCTCTCTGTTTGTTCA+TGG - chr1_4:42515571-42515590 None:intergenic 30.0%
TGAAAAGCTTAACTGAATTG+TGG + chr1_4:42515620-42515639 Msa0163970:intron 30.0%
GTTGAAATCTAAATCTAGGA+AGG - chr1_4:42516122-42516141 None:intergenic 30.0%
!! TTTCATAGATATCGGCTTTA+AGG + chr1_4:42516153-42516172 Msa0163970:intron 30.0%
AATAGTCTCGTAAGTGTTTA+TGG - chr1_4:42516214-42516233 None:intergenic 30.0%
TAAACACTTACGAGACTATT+TGG + chr1_4:42516214-42516233 Msa0163970:intron 30.0%
TTTATTTCCATATGTTCTCC+AGG + chr1_4:42516275-42516294 Msa0163970:intron 30.0%
TTATTTCCATATGTTCTCCA+GGG + chr1_4:42516276-42516295 Msa0163970:intron 30.0%
ACAGTTGAGAAATAAGTTAG+AGG - chr1_4:42516624-42516643 None:intergenic 30.0%
! CTTATTTCTCAACTGTGTAT+AGG + chr1_4:42516628-42516647 Msa0163970:intron 30.0%
!!! TACTCTTTTGAAGCCTAATA+TGG + chr1_4:42517056-42517075 Msa0163970:CDS 30.0%
TCTTACATTCTCTTATGGAA+GGG + chr1_4:42517266-42517285 Msa0163970:CDS 30.0%
!!! GTGTTTGTTTTTGTTTAGGT+TGG + chr1_4:42517559-42517578 Msa0163970:three_prime_UTR 30.0%
GTTGTGTGAAAAATGTATGA+TGG - chr1_4:42517674-42517693 None:intergenic 30.0%
! AAAAAGTGTAAAGCAGAAAG+TGG + chr1_4:42517755-42517774 Msa0163970:three_prime_UTR 30.0%
! AAAAGTGTAAAGCAGAAAGT+GGG + chr1_4:42517756-42517775 Msa0163970:three_prime_UTR 30.0%
! TAGAATGATGGAAAAACAAG+TGG - chr1_4:42517846-42517865 None:intergenic 30.0%
ACTTTCAGTACATAGAATGA+TGG - chr1_4:42517858-42517877 None:intergenic 30.0%
AGAAGACAAGTAGTAAGAAG+AGG - chr1_4:42514263-42514282 None:intergenic 35.0%
CTTGAAAGAAGACATGTTAG+TGG - chr1_4:42514359-42514378 None:intergenic 35.0%
TCTTTCAAGAGCAAGTATGA+AGG + chr1_4:42514370-42514389 Msa0163970:CDS 35.0%
TAGGACATGTTTACCTTTGT+TGG + chr1_4:42514457-42514476 Msa0163970:intron 35.0%
CATGAATGATCAACCAACAA+AGG - chr1_4:42514473-42514492 None:intergenic 35.0%
! CACTAGCTTCATTTAGGTAT+TGG + chr1_4:42514496-42514515 Msa0163970:intron 35.0%
TATACTCTGATGATCCTAGA+TGG + chr1_4:42514565-42514584 Msa0163970:intron 35.0%
! GGAGAACTAGTTGAGTATTT+TGG + chr1_4:42514681-42514700 Msa0163970:intron 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG - chr1_4:42514940-42514959 None:intergenic 35.0%
AGAGAGAAAAATGAAGAGAG+AGG - chr1_4:42514997-42515016 None:intergenic 35.0%
GACAAAATTACGGTTGAATG+TGG + chr1_4:42515257-42515276 Msa0163970:intron 35.0%
! AGTTTTTGCCAAAATTACCG+TGG + chr1_4:42515302-42515321 Msa0163970:intron 35.0%
!!! GTTTGAATTGGTAGCGATTT+TGG - chr1_4:42515343-42515362 None:intergenic 35.0%
AACATACACTAAGTTTGTGG+TGG + chr1_4:42515360-42515379 Msa0163970:intron 35.0%
! AGTTTGTGGTGGAAGAAATA+AGG + chr1_4:42515371-42515390 Msa0163970:intron 35.0%
TGTGAATCAAAATGAGAGCT+AGG + chr1_4:42515667-42515686 Msa0163970:intron 35.0%
AATTGTGTCCTTACTTCTTG+TGG + chr1_4:42515697-42515716 Msa0163970:intron 35.0%
TTAGAAAGTAAGGACATGGA+AGG - chr1_4:42516457-42516476 None:intergenic 35.0%
TTCTTCAACACATCCACAAA+GGG - chr1_4:42516658-42516677 None:intergenic 35.0%
TTTCTTCAACACATCCACAA+AGG - chr1_4:42516659-42516678 None:intergenic 35.0%
ACCATTCTCTTGACATATGA+CGG - chr1_4:42516905-42516924 None:intergenic 35.0%
CATATGTCAAGAGAATGGTT+TGG + chr1_4:42516906-42516925 Msa0163970:CDS 35.0%
AGAATCTCAGGTTCTACAAT+TGG - chr1_4:42516934-42516953 None:intergenic 35.0%
TACCTTCAAGAAGAACATGA+TGG - chr1_4:42517038-42517057 None:intergenic 35.0%
TAAGAGAATGTAAGAGTCCA+AGG - chr1_4:42517261-42517280 None:intergenic 35.0%
TGGACTCTTACATTCTCTTA+TGG + chr1_4:42517261-42517280 Msa0163970:CDS 35.0%
CTCTTACATTCTCTTATGGA+AGG + chr1_4:42517265-42517284 Msa0163970:CDS 35.0%
! ACTTCAGCAAAGTACTCTTA+AGG + chr1_4:42517290-42517309 Msa0163970:CDS 35.0%
TATCAAGGTAATGCTAACCT+TGG + chr1_4:42517402-42517421 Msa0163970:CDS 35.0%
TTCAGAGTCTCTTCATGTTA+AGG + chr1_4:42517434-42517453 Msa0163970:CDS 35.0%
AATATGTTAAGAGGGTTGTG+AGG + chr1_4:42517469-42517488 Msa0163970:exon 35.0%
!!! TTTTGGAGTTGTATGCATCA+TGG + chr1_4:42517507-42517526 Msa0163970:three_prime_UTR 35.0%
AGTTGTATGCATCATGGTTA+TGG + chr1_4:42517513-42517532 Msa0163970:three_prime_UTR 35.0%
!!! TGGTTATGGTGCTATTTTGT+AGG + chr1_4:42517527-42517546 Msa0163970:three_prime_UTR 35.0%
!!! GGTTATGGTGCTATTTTGTA+GGG + chr1_4:42517528-42517547 Msa0163970:three_prime_UTR 35.0%
!!! GTTTTTGTTTAGGTTGGTTG+TGG + chr1_4:42517565-42517584 Msa0163970:three_prime_UTR 35.0%
TGGAAAAACAAGTGGAGAAA+AGG - chr1_4:42517838-42517857 None:intergenic 35.0%
ACACTCTTCTCTATCCATCT+AGG - chr1_4:42514582-42514601 None:intergenic 40.0%
GGCAGAATCACTATTTACCA+CGG - chr1_4:42515322-42515341 None:intergenic 40.0%
ACAAACTATGGCGATACTTG+AGG + chr1_4:42515462-42515481 Msa0163970:intron 40.0%
! ATTGCCAATGCTGCTTACAT+TGG + chr1_4:42515804-42515823 Msa0163970:CDS 40.0%
GATGAATCTACTGGCACAAT+CGG + chr1_4:42515855-42515874 Msa0163970:CDS 40.0%
AACATTCTCCACATTGATGC+TGG - chr1_4:42515892-42515911 None:intergenic 40.0%
TGAGGCAATCTAAACAACCA+GGG - chr1_4:42515956-42515975 None:intergenic 40.0%
CTATATCAAAAAACCGCTGC+AGG + chr1_4:42516002-42516021 Msa0163970:CDS 40.0%
AGAATCTGTAATACCTGCAG+CGG - chr1_4:42516018-42516037 None:intergenic 40.0%
!!! TATCGGCTTTAAGGCCTTTT+TGG + chr1_4:42516162-42516181 Msa0163970:intron 40.0%
TTGAGATGCAAATGCATCAC+AGG - chr1_4:42516555-42516574 None:intergenic 40.0%
AGAAACGACAACTCAAGGTT+TGG + chr1_4:42516741-42516760 Msa0163970:CDS 40.0%
!! TGCAAGAAGTACTATGAAGC+CGG + chr1_4:42516781-42516800 Msa0163970:CDS 40.0%
ATTTGGACCGATCTTTAGCA+CGG - chr1_4:42516830-42516849 None:intergenic 40.0%
TGTAGAACCTGAGATTCTAG+TGG + chr1_4:42516936-42516955 Msa0163970:CDS 40.0%
TGTTCTTGCAGCATGTTACA+AGG + chr1_4:42517002-42517021 Msa0163970:CDS 40.0%
GACCATCATGTTCTTCTTGA+AGG + chr1_4:42517033-42517052 Msa0163970:CDS 40.0%
AAGCCTAATATGGTTACACC+AGG + chr1_4:42517066-42517085 Msa0163970:CDS 40.0%
GATCCTGGTGTAACCATATT+AGG - chr1_4:42517072-42517091 None:intergenic 40.0%
ATAACCTCAGGAGCAACTTT+AGG - chr1_4:42517102-42517121 None:intergenic 40.0%
CCAGACAAGAAAACTATAGC+AGG - chr1_4:42517174-42517193 None:intergenic 40.0%
! CCTGCTATAGTTTTCTTGTC+TGG + chr1_4:42517171-42517190 Msa0163970:CDS 40.0%
! GCTATAGTTTTCTTGTCTGG+TGG + chr1_4:42517174-42517193 Msa0163970:CDS 40.0%
AACTTAAGGGAAAGAAGCCT+TGG + chr1_4:42517241-42517260 Msa0163970:CDS 40.0%
! AGCAAAGTACTCTTAAGGCA+TGG + chr1_4:42517295-42517314 Msa0163970:CDS 40.0%
! GCAAAGTACTCTTAAGGCAT+GGG + chr1_4:42517296-42517315 Msa0163970:CDS 40.0%
! CAAAGTACTCTTAAGGCATG+GGG + chr1_4:42517297-42517316 Msa0163970:CDS 40.0%
! GCAACTCTAGGAACTTATCA+AGG + chr1_4:42517387-42517406 Msa0163970:CDS 40.0%
!! GGCTCTGATTGAACTTGTTT+TGG + chr1_4:42517490-42517509 Msa0163970:three_prime_UTR 40.0%
ATAATGGCTTCAAGCTGAAC+TGG + chr1_4:42517598-42517617 Msa0163970:three_prime_UTR 40.0%
AGTAAGATTCCGGTCTTGCT+TGG + chr1_4:42515154-42515173 Msa0163970:intron 45.0%
GTAAGATTCCGGTCTTGCTT+GGG + chr1_4:42515155-42515174 Msa0163970:intron 45.0%
TAACCAATCCCAAGCAAGAC+CGG - chr1_4:42515166-42515185 None:intergenic 45.0%
TTCCGTTAGCAACTAGCAGT+TGG + chr1_4:42515209-42515228 Msa0163970:intron 45.0%
CACCAACTGCTAGTTGCTAA+CGG - chr1_4:42515214-42515233 None:intergenic 45.0%
TCAGCACTCCACAAGAAGTA+AGG - chr1_4:42515708-42515727 None:intergenic 45.0%
AGAGCCAATGTAAGCAGCAT+TGG - chr1_4:42515811-42515830 None:intergenic 45.0%
!! TTACATTGGCTCTCCTGGAA+AGG + chr1_4:42515818-42515837 Msa0163970:CDS 45.0%
!! TACATTGGCTCTCCTGGAAA+GGG + chr1_4:42515819-42515838 Msa0163970:CDS 45.0%
CAGTAGATTCATCAGCAGCA+AGG - chr1_4:42515848-42515867 None:intergenic 45.0%
CTTGCTGCTGATGAATCTAC+TGG + chr1_4:42515846-42515865 Msa0163970:CDS 45.0%
GAGGCAATCTAAACAACCAG+GGG - chr1_4:42515955-42515974 None:intergenic 45.0%
CTGAGGCAATCTAAACAACC+AGG - chr1_4:42515957-42515976 None:intergenic 45.0%
GGTTGTTTAGATTGCCTCAG+TGG + chr1_4:42515957-42515976 Msa0163970:CDS 45.0%
CGAACAAGATAACACCACTG+AGG - chr1_4:42515974-42515993 None:intergenic 45.0%
ATGCTACCCTGGAGAACATA+TGG - chr1_4:42516285-42516304 None:intergenic 45.0%
GTAAGGACATGGAAGGACTA+AGG - chr1_4:42516450-42516469 None:intergenic 45.0%
TAGATGATTGATGAGGTCCG+AGG + chr1_4:42516501-42516520 Msa0163970:intron 45.0%
! TTCCGACTAAGTTGACACCT+CGG - chr1_4:42516521-42516540 None:intergenic 45.0%
GATGCAAATGCATCACAGGA+AGG - chr1_4:42516551-42516570 None:intergenic 45.0%
!! GTGTATAGGAGTACCCTTTG+TGG + chr1_4:42516642-42516661 Msa0163970:intron 45.0%
GTGGATGTGTTGAAGAAAGC+TGG + chr1_4:42516661-42516680 Msa0163970:CDS 45.0%
AAGAAAGCTGGAGTTCTTCC+TGG + chr1_4:42516673-42516692 Msa0163970:CDS 45.0%
TGGAGTTCTTCCTGGTATCA+AGG + chr1_4:42516681-42516700 Msa0163970:CDS 45.0%
TCCTGGTATCAAGGTTGACA+AGG + chr1_4:42516690-42516709 Msa0163970:CDS 45.0%
CCTGGTATCAAGGTTGACAA+GGG + chr1_4:42516691-42516710 Msa0163970:CDS 45.0%
AAGGGAACCATTGAACTTGC+CGG + chr1_4:42516709-42516728 Msa0163970:CDS 45.0%
ATTGAACTTGCCGGCACAAA+CGG + chr1_4:42516718-42516737 Msa0163970:CDS 45.0%
AACGGAGAAACGACAACTCA+AGG + chr1_4:42516736-42516755 Msa0163970:CDS 45.0%
ACTCAAGGTTTGGATGACCT+TGG + chr1_4:42516751-42516770 Msa0163970:CDS 45.0%
GACAAGGGAGATGGTTCATT+TGG - chr1_4:42516847-42516866 None:intergenic 45.0%
!! GCGTTTTCGTGGATTGACAA+GGG - chr1_4:42516862-42516881 None:intergenic 45.0%
TCAATCCACGAAAACGCCTA+CGG + chr1_4:42516865-42516884 Msa0163970:CDS 45.0%
GCCGTCATATGTCAAGAGAA+TGG + chr1_4:42516901-42516920 Msa0163970:CDS 45.0%
GAACCTGAGATTCTAGTGGA+TGG + chr1_4:42516940-42516959 Msa0163970:CDS 45.0%
GATCCATCCACTAGAATCTC+AGG - chr1_4:42516946-42516965 None:intergenic 45.0%
! AGCGCATTTTGCGATATCGT+GGG - chr1_4:42516968-42516987 None:intergenic 45.0%
ACATGCTGCAAGAACACGTT+CGG - chr1_4:42516998-42517017 None:intergenic 45.0%
! ACTTTAGGAGAGTCTGATCC+TGG - chr1_4:42517087-42517106 None:intergenic 45.0%
GAAAACTATAGCAGGAACCG+CGG - chr1_4:42517166-42517185 None:intergenic 45.0%
AGCAATGCAGCTTGAGCTTT+AGG - chr1_4:42517342-42517361 None:intergenic 45.0%
CTCAAGCTGCATTGCTTGTT+AGG + chr1_4:42517346-42517365 Msa0163970:CDS 45.0%
GCAAACTCAGAAGCAACTCT+AGG + chr1_4:42517375-42517394 Msa0163970:CDS 45.0%
GGTAATGCTAACCTTGGTGA+TGG + chr1_4:42517408-42517427 Msa0163970:CDS 45.0%
!! TCAAGCTGAACTGGCTTGAT+TGG + chr1_4:42517607-42517626 Msa0163970:three_prime_UTR 45.0%
ATTCCGGTCTTGCTTGGGAT+TGG + chr1_4:42515160-42515179 Msa0163970:intron 50.0%
GCGATTTGCCAGCATCAATG+TGG + chr1_4:42515881-42515900 Msa0163970:CDS 50.0%
! GAACTTTTGTTCACTGCCCC+TGG + chr1_4:42515936-42515955 Msa0163970:CDS 50.0%
TCCAGGGTAGCATAGCAAAG+CGG + chr1_4:42516292-42516311 Msa0163970:intron 50.0%
CGTGCGGTAGATGATTGATG+AGG + chr1_4:42516494-42516513 Msa0163970:intron 50.0%
! GTCCGAGGTGTCAACTTAGT+CGG + chr1_4:42516516-42516535 Msa0163970:intron 50.0%
CCCTTGTCAACCTTGATACC+AGG - chr1_4:42516694-42516713 None:intergenic 50.0%
! GTTTGTGCCGGCAAGTTCAA+TGG - chr1_4:42516719-42516738 None:intergenic 50.0%
! TGTCGTTTCTCCGTTTGTGC+CGG - chr1_4:42516731-42516750 None:intergenic 50.0%
GTTTGGATGACCTTGGTCAG+AGG + chr1_4:42516758-42516777 Msa0163970:CDS 50.0%
ACTTCTTGCACCTCTGACCA+AGG - chr1_4:42516771-42516790 None:intergenic 50.0%
AGTACTATGAAGCCGGTGCA+AGG + chr1_4:42516788-42516807 Msa0163970:CDS 50.0%
CCATTTAGCGAACCTTGCAC+CGG - chr1_4:42516803-42516822 None:intergenic 50.0%
! TCGTGGATTGACAAGGGAGA+TGG - chr1_4:42516856-42516875 None:intergenic 50.0%
!! GGCGTTTTCGTGGATTGACA+AGG - chr1_4:42516863-42516882 None:intergenic 50.0%
! CTAAACCGTAGGCGTTTTCG+TGG - chr1_4:42516873-42516892 None:intergenic 50.0%
GGCATAACGAGCTAAACCGT+AGG - chr1_4:42516884-42516903 None:intergenic 50.0%
! CAGCGCATTTTGCGATATCG+TGG - chr1_4:42516969-42516988 None:intergenic 50.0%
CTCTCCTAAAGTTGCTCCTG+AGG + chr1_4:42517095-42517114 Msa0163970:CDS 50.0%
GTGTGTTGGGCTATAACCTC+AGG - chr1_4:42517114-42517133 None:intergenic 50.0%
CAAAGCTCGGACTGTGTGTT+GGG - chr1_4:42517127-42517146 None:intergenic 50.0%
ACAAAGCTCGGACTGTGTGT+TGG - chr1_4:42517128-42517147 None:intergenic 50.0%
GAACGGTTCGCAACAAAGCT+CGG - chr1_4:42517140-42517159 None:intergenic 50.0%
! GTCTGGTGGACAAAGTGAAG+AGG + chr1_4:42517188-42517207 Msa0163970:CDS 50.0%
AGTACTCTTAAGGCATGGGG+TGG + chr1_4:42517300-42517319 Msa0163970:CDS 50.0%
!! ACTCTGAAGCACCATCACCA+AGG - chr1_4:42517422-42517441 None:intergenic 50.0%
!! GCTGCTTACATTGGCTCTCC+TGG + chr1_4:42515813-42515832 Msa0163970:CDS 55.0%
GCAGCAAGGATACCCTTTCC+AGG - chr1_4:42515834-42515853 None:intergenic 55.0%
! ACCGCTTTGCTATGCTACCC+TGG - chr1_4:42516296-42516315 None:intergenic 55.0%
CCGGTGCAAGGTTCGCTAAA+TGG + chr1_4:42516800-42516819 Msa0163970:CDS 55.0%
TGGCGTGCCGTGCTAAAGAT+CGG + chr1_4:42516820-42516839 Msa0163970:CDS 55.0%
ACTATAGCAGGAACCGCGGC+AGG - chr1_4:42517162-42517181 None:intergenic 60.0%
GTTGCGAACCGTTCCTGCCG+CGG + chr1_4:42517146-42517165 Msa0163970:CDS 65.0%
AGCAGGAACCGCGGCAGGAA+CGG - chr1_4:42517157-42517176 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 42514173 42517878 42514173 ID=Msa0163970;Name=Msa0163970
chr1_4 mRNA 42514173 42517878 42514173 ID=Msa0163970-mRNA-1;Parent=Msa0163970;Name=Msa0163970-mRNA-1;_AED=0.01;_eAED=0.01;_QI=191|1|1|1|1|1|3|408|358
chr1_4 exon 42514173 42514391 42514173 ID=Msa0163970-mRNA-1:exon:12311;Parent=Msa0163970-mRNA-1
chr1_4 exon 42515796 42516023 42515796 ID=Msa0163970-mRNA-1:exon:12312;Parent=Msa0163970-mRNA-1
chr1_4 exon 42516650 42517878 42516650 ID=Msa0163970-mRNA-1:exon:12313;Parent=Msa0163970-mRNA-1
chr1_4 five_prime_UTR 42514173 42514363 42514173 ID=Msa0163970-mRNA-1:five_prime_utr;Parent=Msa0163970-mRNA-1
chr1_4 CDS 42514364 42514391 42514364 ID=Msa0163970-mRNA-1:cds;Parent=Msa0163970-mRNA-1
chr1_4 CDS 42515796 42516023 42515796 ID=Msa0163970-mRNA-1:cds;Parent=Msa0163970-mRNA-1
chr1_4 CDS 42516650 42517470 42516650 ID=Msa0163970-mRNA-1:cds;Parent=Msa0163970-mRNA-1
chr1_4 three_prime_UTR 42517471 42517878 42517471 ID=Msa0163970-mRNA-1:three_prime_utr;Parent=Msa0163970-mRNA-1
Gene Sequence

>Msa0163970

ATGTCTTCTTTCAAGAGCAAGTATGAAGATGAACTCATTGCCAATGCTGCTTACATTGGCTCTCCTGGAAAGGGTATCCTTGCTGCTGATGAATCTACTGGCACAATCGGCAAGCGATTTGCCAGCATCAATGTGGAGAATGTTGAAGCAAACAGACGTACTCTTCGCGAACTTTTGTTCACTGCCCCTGGTTGTTTAGATTGCCTCAGTGGTGTTATCTTGTTCGAAGAAACACTATATCAAAAAACCGCTGCAGGAGTACCCTTTGTGGATGTGTTGAAGAAAGCTGGAGTTCTTCCTGGTATCAAGGTTGACAAGGGAACCATTGAACTTGCCGGCACAAACGGAGAAACGACAACTCAAGGTTTGGATGACCTTGGTCAGAGGTGCAAGAAGTACTATGAAGCCGGTGCAAGGTTCGCTAAATGGCGTGCCGTGCTAAAGATCGGTCCAAATGAACCATCTCCCTTGTCAATCCACGAAAACGCCTACGGTTTAGCTCGTTATGCCGTCATATGTCAAGAGAATGGTTTGGTTCCAATTGTAGAACCTGAGATTCTAGTGGATGGATCCCACGATATCGCAAAATGCGCTGATGTAACCGAACGTGTTCTTGCAGCATGTTACAAGGCTCTAAGTGACCATCATGTTCTTCTTGAAGGTACTCTTTTGAAGCCTAATATGGTTACACCAGGATCAGACTCTCCTAAAGTTGCTCCTGAGGTTATAGCCCAACACACAGTCCGAGCTTTGTTGCGAACCGTTCCTGCCGCGGTTCCTGCTATAGTTTTCTTGTCTGGTGGACAAAGTGAAGAGGAAGCAACTATTAATCTTAATGCAATGAACAAACTTAAGGGAAAGAAGCCTTGGACTCTTACATTCTCTTATGGAAGGGCACTTCAGCAAAGTACTCTTAAGGCATGGGGTGGAAAAGATGAAAACATTCCTAAAGCTCAAGCTGCATTGCTTGTTAGGTGTAAAGCAAACTCAGAAGCAACTCTAGGAACTTATCAAGGTAATGCTAACCTTGGTGATGGTGCTTCAGAGTCTCTTCATGTTAAGGACTACAAATACTAA
Protein sequence

>Msa0163970

MSSFKSKYEDELIANAAYIGSPGKGILAADESTGTIGKRFASINVENVEANRRTLRELLFTAPGCLDCLSGVILFEETLYQKTAAGVPFVDVLKKAGVLPGIKVDKGTIELAGTNGETTTQGLDDLGQRCKKYYEAGARFAKWRAVLKIGPNEPSPLSIHENAYGLARYAVICQENGLVPIVEPEILVDGSHDIAKCADVTERVLAACYKALSDHHVLLEGTLLKPNMVTPGSDSPKVAPEVIAQHTVRALLRTVPAAVPAIVFLSGGQSEEEATINLNAMNKLKGKKPWTLTFSYGRALQQSTLKAWGGKDENIPKAQAALLVRCKANSEATLGTYQGNANLGDGASESLHVKDYKY*