AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0167870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0167870 MtrunA17_Chr1g0183841 100.000 205 0 0 1 205 1 205 4.61e-157 432
Msa0167870 MtrunA17_Chr1g0183821 80.976 205 39 0 1 205 1 205 3.64e-122 344
Msa0167870 MtrunA17_Chr1g0183831 83.065 124 20 1 82 205 18 140 1.65e-69 208
Msa0167870 MtrunA17_Chr1g0183801 64.286 84 24 1 122 205 12 89 2.18e-29 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0167870 AT2G31140.1 57.073 205 88 0 1 205 1 205 7.37e-87 255
Msa0167870 AT1G06200.1 56.585 205 89 0 1 205 1 205 2.43e-86 254
Msa0167870 AT1G06200.2 56.585 205 89 0 1 205 38 242 1.08e-85 253

Find 57 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCGATGGTGTATTGAGCTT+TGG 0.370737 1_4:-49923273 None:intergenic
CAGCATTTCGCAGGCAGTAT+AGG 0.382636 1_4:-49926029 None:intergenic
GCTCCTTTGGCATCAATAGT+AGG 0.391625 1_4:-49923573 None:intergenic
GCAATTGACAGTCGAACATT+TGG 0.396826 1_4:+49925973 Msa0167870:CDS
TTTGATTGACAGAATCATAA+AGG 0.398944 1_4:+49923450 Msa0167870:intron
TCCTTTAGTCCAATGCAAAA+AGG 0.413433 1_4:-49923538 None:intergenic
CTGTGTTCGTTCTCCAACAA+TGG 0.421436 1_4:+49923217 Msa0167870:exon
GCTTCTCTGAACTAAAGTTC+AGG 0.425492 1_4:-49926237 None:intergenic
CAGTCCTTTCAGTATGCAAA+AGG 0.439464 1_4:+49926157 Msa0167870:intron
TGTGAAAGATAATGAGCTAA+TGG 0.443389 1_4:+49923479 Msa0167870:CDS
ACGATGTTGGTCATGTGAAT+TGG 0.448954 1_4:-49925997 None:intergenic
GGATTCACCAGTGTTGGAAA+TGG 0.450418 1_4:+49926178 Msa0167870:CDS
TGTGTTGTAAGCTGAGGTTC+TGG 0.459240 1_4:+49926450 Msa0167870:three_prime_UTR
ACCATGGCCGTGTACAGAAC+AGG 0.474301 1_4:+49926055 Msa0167870:CDS
AACAAGAAGAGTAGCTCCTT+TGG 0.474364 1_4:-49923586 None:intergenic
TGCGACTGAAGGTTATGAAA+TGG 0.475975 1_4:+49924796 Msa0167870:CDS
CTCCTTTGGCATCAATAGTA+GGG 0.480449 1_4:-49923572 None:intergenic
TTTAAATTGTTGCTATGGGA+TGG 0.482887 1_4:+49926338 Msa0167870:three_prime_UTR
AATGGCTTCTACTGATGAAA+AGG 0.484277 1_4:+49924814 Msa0167870:CDS
CACCATCGATCTCGGCTACA+AGG 0.488299 1_4:+49923286 Msa0167870:CDS
GAGAAGAAAGGAAAATAAGC+TGG 0.491539 1_4:-49926597 None:intergenic
TGTATTGAGCTTTGGATTGA+AGG 0.493410 1_4:-49923265 None:intergenic
GCAAAAGGATTCACCAGTGT+TGG 0.493784 1_4:+49926172 Msa0167870:CDS
TTTCTTCTCTGCTTAATGAC+AGG 0.496700 1_4:+49926611 Msa0167870:three_prime_UTR
TCTTGAGAAGGATCAGTGTT+GGG 0.510455 1_4:+49924850 Msa0167870:CDS
CGAATCGTTTGTTCTTGAGA+AGG 0.511595 1_4:+49924838 Msa0167870:CDS
ACCTGTTCTGTACACGGCCA+TGG 0.512549 1_4:-49926056 None:intergenic
TTTGACAGACGTGTTTATGT+AGG 0.513138 1_4:+49924713 Msa0167870:intron
CTTCTGACTAGATAGTCGTC+TGG 0.513958 1_4:-49924767 None:intergenic
TAAAATCCACAAGAAACAAA+AGG 0.518398 1_4:-49926282 None:intergenic
TTCTTGAGAAGGATCAGTGT+TGG 0.523682 1_4:+49924849 Msa0167870:CDS
TTCTCTGCTTAATGACAGGA+AGG 0.530468 1_4:+49926615 Msa0167870:three_prime_UTR
GTTGTGAAGGAAACCATTGT+TGG 0.532107 1_4:-49923230 None:intergenic
TTGATTACCTGTTCTGTACA+CGG 0.535222 1_4:-49926062 None:intergenic
GCTCAATACACCATCGATCT+CGG 0.545489 1_4:+49923278 Msa0167870:CDS
TGTCATTAAGCAGAGAAGAA+AGG 0.547378 1_4:-49926609 None:intergenic
TTAAACTAAATCTGTAACAC+AGG 0.555523 1_4:-49926424 None:intergenic
GAAGGATCAGTGTTGGGTAG+TGG 0.557582 1_4:+49924856 Msa0167870:CDS
GACCTTGTAGCCGAGATCGA+TGG 0.558078 1_4:-49923288 None:intergenic
TTGGACTAAAGGAGAGGAGA+TGG 0.566801 1_4:+49923548 Msa0167870:CDS
CATGGTCTACAGCATTTCGC+AGG 0.571516 1_4:-49926038 None:intergenic
TAGGACTCTGCCTACGATGT+TGG 0.572719 1_4:-49926010 None:intergenic
TCAAGTTCCATTTCCAACAC+TGG 0.579157 1_4:-49926185 None:intergenic
TAACATTGTGTTGTAAGCTG+AGG 0.583043 1_4:+49926444 Msa0167870:three_prime_UTR
AGAAGATTAGCTGCGACTGA+AGG 0.584300 1_4:+49924785 Msa0167870:CDS
CCCAACCAGAAAATCTGAAG+CGG 0.584646 1_4:+49926401 Msa0167870:three_prime_UTR
GGAACTTGATGTTGATGAGA+TGG 0.587426 1_4:+49926199 Msa0167870:CDS
GCCCCTACTATTGATGCCAA+AGG 0.592866 1_4:+49923570 Msa0167870:CDS
AAATGAGAAATTGAAAGCCA+AGG 0.607182 1_4:+49924883 Msa0167870:CDS
TGTTTATGTAGGAGATGTTG+TGG 0.623948 1_4:+49924724 Msa0167870:CDS
TTTGCATTGGACTAAAGGAG+AGG 0.625454 1_4:+49923542 Msa0167870:CDS
TCCTTTGGCATCAATAGTAG+GGG 0.637535 1_4:-49923571 None:intergenic
GTAGCGACACCACGTTGTGA+AGG 0.640169 1_4:-49923243 None:intergenic
ATTCACATGACCAACATCGT+AGG 0.663772 1_4:+49926000 Msa0167870:CDS
GATTGACAGAATCATAAAGG+AGG 0.664267 1_4:+49923453 Msa0167870:intron
CAATGGTTTCCTTCACAACG+TGG 0.665055 1_4:+49923234 Msa0167870:exon
CTGCGAAATGCTGTAGACCA+TGG 0.666500 1_4:+49926039 Msa0167870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTTTTA+CGG + chr1_4:49924447-49924466 Msa0167870:intron 0.0%
!! TTAAATTTGGATTTAAATAT+TGG + chr1_4:49924134-49924153 Msa0167870:intron 10.0%
!!! TATTTTATTTGTTAAGAAAA+GGG - chr1_4:49925303-49925322 None:intergenic 10.0%
!!! TTATTTTATTTGTTAAGAAA+AGG - chr1_4:49925304-49925323 None:intergenic 10.0%
!! TATAATCTTATATTGTATAT+TGG + chr1_4:49925866-49925885 Msa0167870:intron 10.0%
!! ATTAGATATGATTTATGATA+GGG + chr1_4:49924160-49924179 Msa0167870:intron 15.0%
!!! TCAATTTCAAAATTTGAAAT+TGG + chr1_4:49924555-49924574 Msa0167870:intron 15.0%
!!! ATTTTATTTGTTAAGAAAAG+GGG - chr1_4:49925302-49925321 None:intergenic 15.0%
!! TACAATTAAAGCTAATATTT+AGG - chr1_4:49925647-49925666 None:intergenic 15.0%
!! TTTCTATTAATACTTAGATA+TGG - chr1_4:49925828-49925847 None:intergenic 15.0%
!!! TCTTTTTTCATTTCTCTTTT+AGG + chr1_4:49923332-49923351 Msa0167870:intron 20.0%
!!! CTTTTTTCATTTCTCTTTTA+GGG + chr1_4:49923333-49923352 Msa0167870:intron 20.0%
!!! TATGATTAATTACTTTTGGT+CGG - chr1_4:49923628-49923647 None:intergenic 20.0%
!!! TGAGTATGATTAATTACTTT+TGG - chr1_4:49923632-49923651 None:intergenic 20.0%
!! CATTAGATATGATTTATGAT+AGG + chr1_4:49924159-49924178 Msa0167870:intron 20.0%
!! TTATGAAGTTATAAATTAGC+TGG + chr1_4:49924368-49924387 Msa0167870:intron 20.0%
!!! TAAAATTGTTTTGAAGCTAA+TGG + chr1_4:49924476-49924495 Msa0167870:intron 20.0%
!! AAAGTTAGTTAAGAGATTAT+CGG + chr1_4:49924617-49924636 Msa0167870:intron 20.0%
!!! ATTACTTTTATGCTATCAAT+AGG + chr1_4:49924953-49924972 Msa0167870:intron 20.0%
!!! TTAACTAATGGTTTGATTTT+TGG + chr1_4:49925175-49925194 Msa0167870:intron 20.0%
!! CTATTGACAAATGTAAAAAT+TGG - chr1_4:49925220-49925239 None:intergenic 20.0%
!!! TTTTATTTGTTAAGAAAAGG+GGG - chr1_4:49925301-49925320 None:intergenic 20.0%
!!! TCAGGTTCAAATAATTTTAT+CGG - chr1_4:49925748-49925767 None:intergenic 20.0%
!! AAAAACTAAAAAGACAGAAA+AGG - chr1_4:49925780-49925799 None:intergenic 20.0%
!!! TTAATAGAAAAGTTTTCACA+AGG + chr1_4:49925838-49925857 Msa0167870:intron 20.0%
!!! TCTTTGTTTACAAGAAATTA+AGG - chr1_4:49925907-49925926 None:intergenic 20.0%
!!! ATCACAAGTTCTACATTTTT+TGG + chr1_4:49923160-49923179 Msa0167870:five_prime_UTR 25.0%
!!! TCACAAGTTCTACATTTTTT+GGG + chr1_4:49923161-49923180 Msa0167870:five_prime_UTR 25.0%
!!! TATTGTTAAGCTGTTTTGAA+TGG + chr1_4:49923422-49923441 Msa0167870:intron 25.0%
! TTTGATTGACAGAATCATAA+AGG + chr1_4:49923450-49923469 Msa0167870:intron 25.0%
!!! ATTTTCAAGCATTTCACTAA+TGG + chr1_4:49923507-49923526 Msa0167870:CDS 25.0%
!!! ATGATTAATTACTTTTGGTC+GGG - chr1_4:49923627-49923646 None:intergenic 25.0%
!! TAGAGTAAAAGTTTGCATTT+TGG + chr1_4:49923764-49923783 Msa0167870:intron 25.0%
! AGCATTTATTTATAAGTGAG+AGG + chr1_4:49923898-49923917 Msa0167870:intron 25.0%
! GTCAAACAATTGCTTAAATT+TGG + chr1_4:49924121-49924140 Msa0167870:intron 25.0%
! TAGGAATAACAAGAACAAAA+TGG - chr1_4:49924588-49924607 None:intergenic 25.0%
!! AATCTCTTAACTAACTTTTG+TGG - chr1_4:49924615-49924634 None:intergenic 25.0%
! TGTCTATCACTAACAAATAA+TGG - chr1_4:49924687-49924706 None:intergenic 25.0%
! CATTGATATGCATGTATTTA+AGG + chr1_4:49925088-49925107 Msa0167870:intron 25.0%
! TCAATAGATGTTGACATTAT+TGG + chr1_4:49925233-49925252 Msa0167870:intron 25.0%
!! GAATTAAGAATTTTCACTTG+AGG - chr1_4:49925423-49925442 None:intergenic 25.0%
!! TTATCAGGAAAAAGCTATTT+TGG - chr1_4:49925537-49925556 None:intergenic 25.0%
!! TATTTAGGAAGAAACTGTTT+TGG - chr1_4:49925632-49925651 None:intergenic 25.0%
!!! TATAATCCTTTTGTTTCTTG+TGG + chr1_4:49926276-49926295 Msa0167870:three_prime_UTR 25.0%
! TAAAATCCACAAGAAACAAA+AGG - chr1_4:49926285-49926304 None:intergenic 25.0%
!!! TGAGTTTTAAATTGTTGCTA+TGG + chr1_4:49926333-49926352 Msa0167870:three_prime_UTR 25.0%
!!! GAGTTTTAAATTGTTGCTAT+GGG + chr1_4:49926334-49926353 Msa0167870:three_prime_UTR 25.0%
! TTAAACTAAATCTGTAACAC+AGG - chr1_4:49926427-49926446 None:intergenic 25.0%
!! TTTTCTGTTTGTATGTCATT+TGG + chr1_4:49926493-49926512 Msa0167870:three_prime_UTR 25.0%
!!! CACAAGTTCTACATTTTTTG+GGG + chr1_4:49923162-49923181 Msa0167870:five_prime_UTR 30.0%
!!! ACAAGTTCTACATTTTTTGG+GGG + chr1_4:49923163-49923182 Msa0167870:five_prime_UTR 30.0%
TGTGAAAGATAATGAGCTAA+TGG + chr1_4:49923479-49923498 Msa0167870:CDS 30.0%
!! AGTTTGCATTTTGGAAATGA+TGG + chr1_4:49923773-49923792 Msa0167870:intron 30.0%
!! TGTAACAAGTTTGAGTTTTC+TGG + chr1_4:49923841-49923860 Msa0167870:intron 30.0%
!! GTCTAGAAGTCTTATTAGAA+AGG + chr1_4:49923866-49923885 Msa0167870:intron 30.0%
!! TAGAAGTCTTATTAGAAAGG+TGG + chr1_4:49923869-49923888 Msa0167870:intron 30.0%
GATATGATTTATGATAGGGT+AGG + chr1_4:49924164-49924183 Msa0167870:intron 30.0%
TTTCTTGTTACCTATAAGTC+AGG + chr1_4:49924304-49924323 Msa0167870:intron 30.0%
! GTAGAAACAAACGTTTGTTT+TGG + chr1_4:49924335-49924354 Msa0167870:intron 30.0%
!!! ATTGTTTTGAAGCTAATGGA+TGG + chr1_4:49924480-49924499 Msa0167870:intron 30.0%
!!! AGTTGGTAATCTTTTTACAG+TGG - chr1_4:49924528-49924547 None:intergenic 30.0%
TTGAAATTGACACAGTAAGT+TGG - chr1_4:49924545-49924564 None:intergenic 30.0%
! AACTAACTTTTGTGGAACTT+AGG - chr1_4:49924607-49924626 None:intergenic 30.0%
AAATGAGAAATTGAAAGCCA+AGG + chr1_4:49924883-49924902 Msa0167870:CDS 30.0%
ATTGAAAGCCAAGGTATAAA+TGG + chr1_4:49924892-49924911 Msa0167870:intron 30.0%
TCTAAAAGCCATTTATACCT+TGG - chr1_4:49924903-49924922 None:intergenic 30.0%
! ACTTTTATGCTATCAATAGG+TGG + chr1_4:49924956-49924975 Msa0167870:intron 30.0%
! CTTTTATGCTATCAATAGGT+GGG + chr1_4:49924957-49924976 Msa0167870:intron 30.0%
TGATATGCATGTATTTAAGG+CGG + chr1_4:49925091-49925110 Msa0167870:intron 30.0%
GTGATATTAACATGATACCT+AGG + chr1_4:49925151-49925170 Msa0167870:intron 30.0%
TGATACCTAGGTTTAACTAA+TGG + chr1_4:49925163-49925182 Msa0167870:intron 30.0%
TCAAACCATTAGTTAAACCT+AGG - chr1_4:49925171-49925190 None:intergenic 30.0%
! CTATTAACTTAAGCAGCTTT+TGG + chr1_4:49925341-49925360 Msa0167870:intron 30.0%
TTCTGTGTATGACTTTGATA+AGG + chr1_4:49925366-49925385 Msa0167870:intron 30.0%
TATGACTTTGATAAGGTTCA+AGG + chr1_4:49925373-49925392 Msa0167870:intron 30.0%
!!! ATTCCTGTTTTAACCAAACT+AGG + chr1_4:49925439-49925458 Msa0167870:intron 30.0%
!! TATTTAGGAAGTACCTAGTT+TGG - chr1_4:49925455-49925474 None:intergenic 30.0%
CATTGCAGCTACTATTATTT+AGG - chr1_4:49925470-49925489 None:intergenic 30.0%
CTAAATAATAGTAGCTGCAA+TGG + chr1_4:49925468-49925487 Msa0167870:intron 30.0%
! GAATTAAGCATTTTCACTTG+AGG - chr1_4:49925505-49925524 None:intergenic 30.0%
!!! GAAACTGTTTTGGTTAAAAC+AGG - chr1_4:49925622-49925641 None:intergenic 30.0%
CAGAAAAGGACAAAAAATTC+AGG - chr1_4:49925766-49925785 None:intergenic 30.0%
ATTAGTACATTGTGCACAAA+TGG + chr1_4:49926094-49926113 Msa0167870:intron 30.0%
TTTAAATTGTTGCTATGGGA+TGG + chr1_4:49926338-49926357 Msa0167870:three_prime_UTR 30.0%
ACAAACAGAAAAAGAAACAG+AGG - chr1_4:49926485-49926504 None:intergenic 30.0%
!!! CAAGTTCTACATTTTTTGGG+GGG + chr1_4:49923164-49923183 Msa0167870:five_prime_UTR 35.0%
!!! AAGTTCTACATTTTTTGGGG+GGG + chr1_4:49923165-49923184 Msa0167870:five_prime_UTR 35.0%
!! TGTATTGAGCTTTGGATTGA+AGG - chr1_4:49923268-49923287 None:intergenic 35.0%
GATTGACAGAATCATAAAGG+AGG + chr1_4:49923453-49923472 Msa0167870:intron 35.0%
! GAAAGATGACCTTTTTGCAT+TGG + chr1_4:49923529-49923548 Msa0167870:CDS 35.0%
! ACCTTTTTGCATTGGACTAA+AGG + chr1_4:49923537-49923556 Msa0167870:CDS 35.0%
TCCTTTAGTCCAATGCAAAA+AGG - chr1_4:49923541-49923560 None:intergenic 35.0%
!! AATTACTTTTGGTCGGGAAT+CGG - chr1_4:49923621-49923640 None:intergenic 35.0%
! GAAATGATGGCAGAGTTTTA+TGG + chr1_4:49923786-49923805 Msa0167870:intron 35.0%
!! AAGTCTTATTAGAAAGGTGG+AGG + chr1_4:49923872-49923891 Msa0167870:intron 35.0%
ACTCAAAGACATTGGGTTTA+TGG - chr1_4:49923926-49923945 None:intergenic 35.0%
CACTTTCACTCAAAGACATT+GGG - chr1_4:49923933-49923952 None:intergenic 35.0%
ACACTTTCACTCAAAGACAT+TGG - chr1_4:49923934-49923953 None:intergenic 35.0%
TAATTTCTCTCTCACTTGTG+TGG + chr1_4:49923953-49923972 Msa0167870:intron 35.0%
TTCTCCTATTGAGTAATGAG+GGG - chr1_4:49924014-49924033 None:intergenic 35.0%
CTTCTCCTATTGAGTAATGA+GGG - chr1_4:49924015-49924034 None:intergenic 35.0%
TCTTCTCCTATTGAGTAATG+AGG - chr1_4:49924016-49924035 None:intergenic 35.0%
TGTCTGATAGTTACATGTAG+AGG + chr1_4:49924078-49924097 Msa0167870:intron 35.0%
GTCTGATAGTTACATGTAGA+GGG + chr1_4:49924079-49924098 Msa0167870:intron 35.0%
! AACCAAAGTGTTTCCTTACT+AGG - chr1_4:49924222-49924241 None:intergenic 35.0%
TAGTAAGGAAACACTTTGGT+TGG + chr1_4:49924221-49924240 Msa0167870:intron 35.0%
ACAACTACTACCTGACTTAT+AGG - chr1_4:49924317-49924336 None:intergenic 35.0%
! TATAAATTAGCTGGTTCCAG+TGG + chr1_4:49924377-49924396 Msa0167870:intron 35.0%
CATGAAATTAGTGAAACCAC+TGG - chr1_4:49924396-49924415 None:intergenic 35.0%
! GGTAATCTTTTTACAGTGGA+GGG - chr1_4:49924524-49924543 None:intergenic 35.0%
! TGGTAATCTTTTTACAGTGG+AGG - chr1_4:49924525-49924544 None:intergenic 35.0%
GTTAAGAGATTATCGGACAA+TGG + chr1_4:49924624-49924643 Msa0167870:intron 35.0%
TTTGACAGACGTGTTTATGT+AGG + chr1_4:49924713-49924732 Msa0167870:intron 35.0%
TGTTTATGTAGGAGATGTTG+TGG + chr1_4:49924724-49924743 Msa0167870:CDS 35.0%
AATGGCTTCTACTGATGAAA+AGG + chr1_4:49924814-49924833 Msa0167870:CDS 35.0%
TAGATGTTGACATTATTGGC+AGG + chr1_4:49925237-49925256 Msa0167870:intron 35.0%
GTTAAGAAAAGGGGGTTTAA+CGG - chr1_4:49925293-49925312 None:intergenic 35.0%
TTAATAGACGTGCAAAACTG+TGG - chr1_4:49925328-49925347 None:intergenic 35.0%
GTACCTAGTTTGGTTAAAAC+AGG - chr1_4:49925445-49925464 None:intergenic 35.0%
TCACTATTGCAGCTATTATC+AGG - chr1_4:49925552-49925571 None:intergenic 35.0%
! CATTTTCACTTTACGAACTC+AGG - chr1_4:49925592-49925611 None:intergenic 35.0%
! GAAATCATTAGCTTGAGTTC+AGG - chr1_4:49925671-49925690 None:intergenic 35.0%
AAGCTAATGATTTCAGTCGT+TGG + chr1_4:49925677-49925696 Msa0167870:intron 35.0%
! GGCTCTTAAGTTTTTCTATG+TGG + chr1_4:49925698-49925717 Msa0167870:intron 35.0%
TTGATTACCTGTTCTGTACA+CGG - chr1_4:49926065-49926084 None:intergenic 35.0%
! GAATCCTTTTGCATACTGAA+AGG - chr1_4:49926164-49926183 None:intergenic 35.0%
! TGTGGATTTTACTTGAAGCA+AGG + chr1_4:49926294-49926313 Msa0167870:three_prime_UTR 35.0%
TAACATTGTGTTGTAAGCTG+AGG + chr1_4:49926444-49926463 Msa0167870:three_prime_UTR 35.0%
GAGAAGAAAGGAAAATAAGC+TGG - chr1_4:49926600-49926619 None:intergenic 35.0%
TGTCATTAAGCAGAGAAGAA+AGG - chr1_4:49926612-49926631 None:intergenic 35.0%
TTTCTTCTCTGCTTAATGAC+AGG + chr1_4:49926611-49926630 Msa0167870:three_prime_UTR 35.0%
!!! ACATTTTTTGGGGGGGTTTA+AGG + chr1_4:49923172-49923191 Msa0167870:five_prime_UTR 40.0%
!!! CATTTTTTGGGGGGGTTTAA+GGG + chr1_4:49923173-49923192 Msa0167870:five_prime_UTR 40.0%
GTTGTGAAGGAAACCATTGT+TGG - chr1_4:49923233-49923252 None:intergenic 40.0%
ATCGATGGTGTATTGAGCTT+TGG - chr1_4:49923276-49923295 None:intergenic 40.0%
TTTGCATTGGACTAAAGGAG+AGG + chr1_4:49923542-49923561 Msa0167870:CDS 40.0%
TCCTTTGGCATCAATAGTAG+GGG - chr1_4:49923574-49923593 None:intergenic 40.0%
CTCCTTTGGCATCAATAGTA+GGG - chr1_4:49923575-49923594 None:intergenic 40.0%
AACAAGAAGAGTAGCTCCTT+TGG - chr1_4:49923589-49923608 None:intergenic 40.0%
TCCTTTCAATTTGTCGTGTG+TGG + chr1_4:49923670-49923689 Msa0167870:intron 40.0%
ACCACACACGACAAATTGAA+AGG - chr1_4:49923674-49923693 None:intergenic 40.0%
!! GCAGAGTTTTATGGTAGTCT+GGG + chr1_4:49923795-49923814 Msa0167870:intron 40.0%
TTACATGTAGAGGGAGTTCA+AGG + chr1_4:49924088-49924107 Msa0167870:intron 40.0%
GGGAGTTCAAGGAAAACTAT+AGG + chr1_4:49924099-49924118 Msa0167870:intron 40.0%
! CAAAGTGTTTCCTTACTAGG+TGG - chr1_4:49924219-49924238 None:intergenic 40.0%
CACCTAGTAAGGAAACACTT+TGG + chr1_4:49924217-49924236 Msa0167870:intron 40.0%
TCTATTCAAGAACGTGCTCA+AGG + chr1_4:49924253-49924272 Msa0167870:intron 40.0%
CTATTCAAGAACGTGCTCAA+GGG + chr1_4:49924254-49924273 Msa0167870:intron 40.0%
ACTAATTTCATGCTCATCCC+AGG + chr1_4:49924404-49924423 Msa0167870:intron 40.0%
! GAGATGAGAGTGTACATAAG+GGG - chr1_4:49924665-49924684 None:intergenic 40.0%
! GGAGATGAGAGTGTACATAA+GGG - chr1_4:49924666-49924685 None:intergenic 40.0%
! TGGAGATGAGAGTGTACATA+AGG - chr1_4:49924667-49924686 None:intergenic 40.0%
TGCGACTGAAGGTTATGAAA+TGG + chr1_4:49924796-49924815 Msa0167870:CDS 40.0%
CGAATCGTTTGTTCTTGAGA+AGG + chr1_4:49924838-49924857 Msa0167870:CDS 40.0%
TTCTTGAGAAGGATCAGTGT+TGG + chr1_4:49924849-49924868 Msa0167870:CDS 40.0%
TCTTGAGAAGGATCAGTGTT+GGG + chr1_4:49924850-49924869 Msa0167870:CDS 40.0%
TCAATAGGTGGGTCATTGTT+AGG + chr1_4:49924968-49924987 Msa0167870:intron 40.0%
TATTGGCAGGATCTAGATTC+TGG + chr1_4:49925250-49925269 Msa0167870:intron 40.0%
TTCAATGCTCCCAACACATA+TGG + chr1_4:49925944-49925963 Msa0167870:intron 40.0%
GCAATTGACAGTCGAACATT+TGG + chr1_4:49925973-49925992 Msa0167870:CDS 40.0%
ACGATGTTGGTCATGTGAAT+TGG - chr1_4:49926000-49926019 None:intergenic 40.0%
ATTCACATGACCAACATCGT+AGG + chr1_4:49926000-49926019 Msa0167870:CDS 40.0%
CAGTCCTTTCAGTATGCAAA+AGG + chr1_4:49926157-49926176 Msa0167870:intron 40.0%
TCAAGTTCCATTTCCAACAC+TGG - chr1_4:49926188-49926207 None:intergenic 40.0%
! GGAACTTGATGTTGATGAGA+TGG + chr1_4:49926199-49926218 Msa0167870:CDS 40.0%
GCTTCTCTGAACTAAAGTTC+AGG - chr1_4:49926240-49926259 None:intergenic 40.0%
TTCTCTGCTTAATGACAGGA+AGG + chr1_4:49926615-49926634 Msa0167870:three_prime_UTR 40.0%
CTGTGTTCGTTCTCCAACAA+TGG + chr1_4:49923217-49923236 Msa0167870:exon 45.0%
CAATGGTTTCCTTCACAACG+TGG + chr1_4:49923234-49923253 Msa0167870:exon 45.0%
GCTCAATACACCATCGATCT+CGG + chr1_4:49923278-49923297 Msa0167870:CDS 45.0%
TTGGACTAAAGGAGAGGAGA+TGG + chr1_4:49923548-49923567 Msa0167870:CDS 45.0%
GCTCCTTTGGCATCAATAGT+AGG - chr1_4:49923576-49923595 None:intergenic 45.0%
! CTGCAATCGAAGCACTAACA+CGG - chr1_4:49923699-49923718 None:intergenic 45.0%
!! GGCAGAGTTTTATGGTAGTC+TGG + chr1_4:49923794-49923813 Msa0167870:intron 45.0%
! GTTTTATGGTAGTCTGGGTC+CGG + chr1_4:49923800-49923819 Msa0167870:intron 45.0%
! TTTTATGGTAGTCTGGGTCC+GGG + chr1_4:49923801-49923820 Msa0167870:intron 45.0%
ACAACATCTCCACACATACC+CGG - chr1_4:49923822-49923841 None:intergenic 45.0%
ATTGAGTAATGAGGGGAGCA+CGG - chr1_4:49924007-49924026 None:intergenic 45.0%
TGCTCCCCTCATTACTCAAT+AGG + chr1_4:49924007-49924026 Msa0167870:intron 45.0%
ATCAGAACTACCCTCCTTAC+TGG - chr1_4:49924051-49924070 None:intergenic 45.0%
!! AAGGAGGGTAGTTCTGATTG+AGG + chr1_4:49924053-49924072 Msa0167870:intron 45.0%
!! TTCGTCGATTCCACCTAGTA+AGG + chr1_4:49924206-49924225 Msa0167870:intron 45.0%
TGAGCCTTTCTGCAACGAAT+AGG + chr1_4:49924277-49924296 Msa0167870:intron 45.0%
GCAACCTATTCGTTGCAGAA+AGG - chr1_4:49924284-49924303 None:intergenic 45.0%
TGAACACATAACCACTGCCT+GGG - chr1_4:49924424-49924443 None:intergenic 45.0%
TTGAACACATAACCACTGCC+TGG - chr1_4:49924425-49924444 None:intergenic 45.0%
!!! GATAGTCGTCTGGCTTTTCA+GGG - chr1_4:49924760-49924779 None:intergenic 45.0%
!!! AGATAGTCGTCTGGCTTTTC+AGG - chr1_4:49924761-49924780 None:intergenic 45.0%
CTTCTGACTAGATAGTCGTC+TGG - chr1_4:49924770-49924789 None:intergenic 45.0%
! AGAAGATTAGCTGCGACTGA+AGG + chr1_4:49924785-49924804 Msa0167870:CDS 45.0%
TTCTGGACATCAGTTGCCAT+CGG + chr1_4:49925267-49925286 Msa0167870:intron 45.0%
ACCCAGATCACAAGCATACA+CGG - chr1_4:49925399-49925418 None:intergenic 45.0%
TAGTAGCTGCAATGGTAACC+TGG + chr1_4:49925476-49925495 Msa0167870:intron 45.0%
AGTAGCTGCAATGGTAACCT+GGG + chr1_4:49925477-49925496 Msa0167870:intron 45.0%
! CATTTTCACTTGAGGAACCC+AGG - chr1_4:49925497-49925516 None:intergenic 45.0%
TGCTTCCTGCCATATGTGTT+GGG - chr1_4:49925956-49925975 None:intergenic 45.0%
TTGCTTCCTGCCATATGTGT+TGG - chr1_4:49925957-49925976 None:intergenic 45.0%
ACATTGTGCACAAATGGCGA+TGG + chr1_4:49926100-49926119 Msa0167870:intron 45.0%
GCAAAAGGATTCACCAGTGT+TGG + chr1_4:49926172-49926191 Msa0167870:CDS 45.0%
! GGATTCACCAGTGTTGGAAA+TGG + chr1_4:49926178-49926197 Msa0167870:CDS 45.0%
!!! CCGCTTCAGATTTTCTGGTT+GGG - chr1_4:49926404-49926423 None:intergenic 45.0%
CCCAACCAGAAAATCTGAAG+CGG + chr1_4:49926401-49926420 Msa0167870:three_prime_UTR 45.0%
TGTGTTGTAAGCTGAGGTTC+TGG + chr1_4:49926450-49926469 Msa0167870:three_prime_UTR 45.0%
!! ATAAAAAAAATACTAAAAAT+TGG - chr1_4:49926535-49926554 None:intergenic 5.0%
!!! AATTTTTAGTATTTTTTTTA+TGG + chr1_4:49926534-49926553 Msa0167870:three_prime_UTR 5.0%
GCCCCTACTATTGATGCCAA+AGG + chr1_4:49923570-49923589 Msa0167870:CDS 50.0%
GTGCTTCGATTGCAGAGACA+CGG + chr1_4:49923704-49923723 Msa0167870:intron 50.0%
CACGGAGGATCATCCACATT+GGG - chr1_4:49923989-49924008 None:intergenic 50.0%
CTCCTTACTGGTGCGTCAAA+GGG - chr1_4:49924039-49924058 None:intergenic 50.0%
CTTTGACGCACCAGTAAGGA+GGG + chr1_4:49924038-49924057 Msa0167870:intron 50.0%
! GAAGGATCAGTGTTGGGTAG+TGG + chr1_4:49924856-49924875 Msa0167870:CDS 50.0%
GCCGTGTATGCTTGTGATCT+GGG + chr1_4:49925395-49925414 Msa0167870:intron 50.0%
ATGCTCCCAACACATATGGC+AGG + chr1_4:49925948-49925967 Msa0167870:intron 50.0%
TAGGACTCTGCCTACGATGT+TGG - chr1_4:49926013-49926032 None:intergenic 50.0%
CAGCATTTCGCAGGCAGTAT+AGG - chr1_4:49926032-49926051 None:intergenic 50.0%
CATGGTCTACAGCATTTCGC+AGG - chr1_4:49926041-49926060 None:intergenic 50.0%
CTGCGAAATGCTGTAGACCA+TGG + chr1_4:49926039-49926058 Msa0167870:CDS 50.0%
!! GCCGCTTCAGATTTTCTGGT+TGG - chr1_4:49926405-49926424 None:intergenic 50.0%
! ACAGGCCGCTTCAGATTTTC+TGG - chr1_4:49926409-49926428 None:intergenic 50.0%
GTAGCGACACCACGTTGTGA+AGG - chr1_4:49923246-49923265 None:intergenic 55.0%
CACCATCGATCTCGGCTACA+AGG + chr1_4:49923286-49923305 Msa0167870:CDS 55.0%
GACCTTGTAGCCGAGATCGA+TGG - chr1_4:49923291-49923310 None:intergenic 55.0%
! TGGTTGCTCTGAGCCCAATG+TGG + chr1_4:49923973-49923992 Msa0167870:intron 55.0%
GCACGGAGGATCATCCACAT+TGG - chr1_4:49923990-49924009 None:intergenic 55.0%
GACCCTTTGACGCACCAGTA+AGG + chr1_4:49924034-49924053 Msa0167870:intron 55.0%
CCTCCTTACTGGTGCGTCAA+AGG - chr1_4:49924040-49924059 None:intergenic 55.0%
CCTTTGACGCACCAGTAAGG+AGG + chr1_4:49924037-49924056 Msa0167870:intron 55.0%
TTCATGCTCATCCCAGGCAG+TGG + chr1_4:49924410-49924429 Msa0167870:intron 55.0%
AAAGGGGGTTTAACGGCCGA+TGG - chr1_4:49925286-49925305 None:intergenic 55.0%
GGCCGTGTATGCTTGTGATC+TGG + chr1_4:49925394-49925413 Msa0167870:intron 55.0%
ACCATGGCCGTGTACAGAAC+AGG + chr1_4:49926055-49926074 Msa0167870:CDS 55.0%
ACCTGTTCTGTACACGGCCA+TGG - chr1_4:49926059-49926078 None:intergenic 55.0%
AGTCTGGGTCCGGGTATGTG+TGG + chr1_4:49923810-49923829 Msa0167870:intron 60.0%
! GAGTAATGAGGGGAGCACGG+AGG - chr1_4:49924004-49924023 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 49923155 49926649 49923155 ID=Msa0167870;Name=Msa0167870
chr1_4 mRNA 49923155 49926649 49923155 ID=Msa0167870-mRNA-1;Parent=Msa0167870;Name=Msa0167870-mRNA-1;_AED=0.08;_eAED=0.08;_QI=81|1|1|1|1|1|5|408|205
chr1_4 exon 49923155 49923307 49923155 ID=Msa0167870-mRNA-1:exon:14725;Parent=Msa0167870-mRNA-1
chr1_4 exon 49923462 49923634 49923462 ID=Msa0167870-mRNA-1:exon:14726;Parent=Msa0167870-mRNA-1
chr1_4 exon 49924721 49924904 49924721 ID=Msa0167870-mRNA-1:exon:14727;Parent=Msa0167870-mRNA-1
chr1_4 exon 49925970 49926076 49925970 ID=Msa0167870-mRNA-1:exon:14728;Parent=Msa0167870-mRNA-1
chr1_4 exon 49926160 49926649 49926160 ID=Msa0167870-mRNA-1:exon:14729;Parent=Msa0167870-mRNA-1
chr1_4 five_prime_UTR 49923155 49923235 49923155 ID=Msa0167870-mRNA-1:five_prime_utr;Parent=Msa0167870-mRNA-1
chr1_4 CDS 49923236 49923307 49923236 ID=Msa0167870-mRNA-1:cds;Parent=Msa0167870-mRNA-1
chr1_4 CDS 49923462 49923634 49923462 ID=Msa0167870-mRNA-1:cds;Parent=Msa0167870-mRNA-1
chr1_4 CDS 49924721 49924904 49924721 ID=Msa0167870-mRNA-1:cds;Parent=Msa0167870-mRNA-1
chr1_4 CDS 49925970 49926076 49925970 ID=Msa0167870-mRNA-1:cds;Parent=Msa0167870-mRNA-1
chr1_4 CDS 49926160 49926241 49926160 ID=Msa0167870-mRNA-1:cds;Parent=Msa0167870-mRNA-1
chr1_4 three_prime_UTR 49926242 49926649 49926242 ID=Msa0167870-mRNA-1:three_prime_utr;Parent=Msa0167870-mRNA-1
Gene Sequence

>Msa0167870

ATGGTTTCCTTCACAACGTGGTGTCGCTACCTTCAATCCAAAGCTCAATACACCATCGATCTCGGCTACAAGAATCATAAAGGAGGTCATGTGAAAGATAATGAGCTAATGGATTTTATTTTCAAGCATTTCACTAATGGAAAGATGACCTTTTTGCATTGGACTAAAGGAGAGGAGATGGCCCCTACTATTGATGCCAAAGGAGCTACTCTTCTTGTTCGTAGATTACCGATTCCCGACCAAAAACGTGTTTATGTAGGAGATGTTGTGGTCTTGAAGAACCCTGAAAAGCCAGACGACTATCTAGTCAGAAGATTAGCTGCGACTGAAGGTTATGAAATGGCTTCTACTGATGAAAAGGACGAATCGTTTGTTCTTGAGAAGGATCAGTGTTGGGTAGTGGCTGAAAATGAGAAATTGAAAGCCAAGGAAGCAATTGACAGTCGAACATTTGGTCCAATTCACATGACCAACATCGTAGGCAGAGTCCTATACTGCCTGCGAAATGCTGTAGACCATGGCCGTGTACAGAACAGTCCTTTCAGTATGCAAAAGGATTCACCAGTGTTGGAAATGGAACTTGATGTTGATGAGATGGTAAAAAGTCACAAAGCCTGA
Protein sequence

>Msa0167870

MVSFTTWCRYLQSKAQYTIDLGYKNHKGGHVKDNELMDFIFKHFTNGKMTFLHWTKGEEMAPTIDAKGATLLVRRLPIPDQKRVYVGDVVVLKNPEKPDDYLVRRLAATEGYEMASTDEKDESFVLEKDQCWVVAENEKLKAKEAIDSRTFGPIHMTNIVGRVLYCLRNAVDHGRVQNSPFSMQKDSPVLEMELDVDEMVKSHKA*