AlfalfaGEDB | Msa0027580

M. sativa cultivar zhongmu-4 / Msa0027580

KEGG: K09647
GO: -
PFAM: PF10502.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0027580	-0.848088	7.657738e-60	-8.615850e-47
Msa0004060	Msa0027580	0.815201	1.043626e-51	-8.615850e-47
Msa0004540	Msa0027580	0.804949	1.689899e-49	-8.615850e-47
Msa0004950	Msa0027580	0.808768	2.632822e-50	-8.615850e-47
Msa0006290	Msa0027580	0.818181	2.241602e-52	-8.615850e-47
Msa0006880	Msa0027580	0.839964	1.156499e-57	-8.615850e-47
Msa0007850	Msa0027580	0.825048	5.797822e-54	-8.615850e-47
Msa0014130	Msa0027580	0.814000	1.925043e-51	-8.615850e-47
Msa0026150	Msa0027580	0.824227	9.047682e-54	-8.615850e-47
Msa0026660	Msa0027580	0.832976	6.951223e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0027580	MtrunA17_Chr1g0183841	99.512	205	1	0	1	205	1	205	1.29e-156	431
Msa0027580	MtrunA17_Chr1g0183821	80.488	205	40	0	1	205	1	205	9.34e-122	343
Msa0027580	MtrunA17_Chr1g0183831	83.065	124	20	1	82	205	18	140	1.50e-69	208
Msa0027580	MtrunA17_Chr1g0183801	64.286	84	24	1	122	205	12	89	2.20e-29	105
Msa0027580	MtrunA17_Chr8g0353441	31.034	145	90	4	39	178	22	161	1.29e-12	63.2
Msa0027580	MtrunA17_Chr8g0353451	29.078	141	90	3	43	178	26	161	2.15e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0027580	AT2G31140.1	57.073	205	88	1	205	1	205	3.46e-87	256
Msa0027580	AT1G06200.1	57.073	205	88	1	205	1	205	1.13e-86	254
Msa0027580	AT1G06200.2	57.073	205	88	1	205	38	242	3.78e-86	254

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Find 57 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAGAAATTGAAAGCCA+AGG	0.607182	1_1:-51942479	Msa0027580:intron
AACAAGAAGAGTAGATCCTT+TGG	0.482482	1_1:+51943680	None:intergenic
AATGGCTTCTACTGATGAAA+AGG	0.484277	1_1:-51942548	Msa0027580:CDS
ACCATGGCCGTGTACAGAAC+AGG	0.474301	1_1:-51941306	Msa0027580:intron
ACCTGTTCTGTACACGGCCA+TGG	0.512549	1_1:+51941305	None:intergenic
ACGATGTTGGTCATGTGAAT+TGG	0.448954	1_1:+51941364	None:intergenic
AGAAGATTAGCTGCGACTGA+AGG	0.584300	1_1:-51942577	Msa0027580:CDS
ATCCTTTGGCATCAATAGTA+GGG	0.497448	1_1:+51943694	None:intergenic
ATCGATGGTGTATTGAGCTT+TGG	0.370737	1_1:+51943993	None:intergenic
ATTCACATGACCAACATCGT+AGG	0.663772	1_1:-51941361	Msa0027580:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTGAAAGATAATGAGCTAA+TGG	-	chr1_1:51941057-51941076	Msa0027580:three_prime_UTR	30.0%
AGCATTTATTTATAAGCGAG+AGG	-	chr1_1:51941476-51941495	Msa0027580:intron	30.0%
TATAGGTCAAACAATTGCTT+AGG	-	chr1_1:51941694-51941713	Msa0027580:intron	30.0%
TTTCTTGTTACCTATAAGTC+AGG	-	chr1_1:51941792-51941811	Msa0027580:intron	30.0%
TTGAAATTGACACAGTAAGT+TGG	+	chr1_1:51942027-51942046	None:intergenic	30.0%
GTTAAGAGATTATCAGACAA+TGG	-	chr1_1:51942106-51942125	Msa0027580:intron	30.0%
AAATGAGAAATTGAAAGCCA+AGG	-	chr1_1:51942365-51942384	Msa0027580:intron	30.0%
ATTGAAAGCCAAGGTATAAA+TGG	-	chr1_1:51942374-51942393	Msa0027580:intron	30.0%
TCTAAAAGCCATTTATACCT+TGG	+	chr1_1:51942385-51942404	None:intergenic	30.0%
TGATATGCATGTATTTAAGG+CGG	-	chr1_1:51942573-51942592	Msa0027580:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	51940733	51944133	51940733	ID=Msa0027580;Name=Msa0027580
chr1_1	mRNA	51940733	51944133	51940733	ID=Msa0027580-mRNA-1;Parent=Msa0027580;Name=Msa0027580-mRNA-1;_AED=0.29;_eAED=0.29;_QI=81\|1\|1\|1\|1\|1\|5\|409\|205
chr1_1	exon	51940733	51941223	51940733	ID=Msa0027580-mRNA-1:exon:15291;Parent=Msa0027580-mRNA-1
chr1_1	exon	51941307	51941413	51941307	ID=Msa0027580-mRNA-1:exon:15290;Parent=Msa0027580-mRNA-1
chr1_1	exon	51942480	51942663	51942480	ID=Msa0027580-mRNA-1:exon:15289;Parent=Msa0027580-mRNA-1
chr1_1	exon	51943654	51943826	51943654	ID=Msa0027580-mRNA-1:exon:15288;Parent=Msa0027580-mRNA-1
chr1_1	exon	51943981	51944133	51943981	ID=Msa0027580-mRNA-1:exon:15287;Parent=Msa0027580-mRNA-1
chr1_1	five_prime_UTR	51944053	51944133	51944053	ID=Msa0027580-mRNA-1:five_prime_utr;Parent=Msa0027580-mRNA-1
chr1_1	CDS	51943981	51944052	51943981	ID=Msa0027580-mRNA-1:cds;Parent=Msa0027580-mRNA-1
chr1_1	CDS	51943654	51943826	51943654	ID=Msa0027580-mRNA-1:cds;Parent=Msa0027580-mRNA-1
chr1_1	CDS	51942480	51942663	51942480	ID=Msa0027580-mRNA-1:cds;Parent=Msa0027580-mRNA-1
chr1_1	CDS	51941307	51941413	51941307	ID=Msa0027580-mRNA-1:cds;Parent=Msa0027580-mRNA-1
chr1_1	CDS	51941142	51941223	51941142	ID=Msa0027580-mRNA-1:cds;Parent=Msa0027580-mRNA-1
chr1_1	three_prime_UTR	51940733	51941141	51940733	ID=Msa0027580-mRNA-1:three_prime_utr;Parent=Msa0027580-mRNA-1

Gene Sequence

>Msa0027580

ATGGTTTCCTTCACAACGTGGTGTCGCTACCTTCAATCCAAAGCTCAATACACCATCGATCTCGGCTACAAGAATCATAAAGGAGGTCATGTGAAAGATAATGAGCTAATGGATTTTATTTTCAAGCATTTCACTAATGGAAAGATGACCTTTTTGCATTGGACTAAAGGAGAGGAGATGGCCCCTACTATTGATGCCAAAGGATCTACTCTTCTTGTTCGTAGATTACCGATTCCCGACCAAAAACGTGTTTATGTAGGAGATGTTGTGGTCTTGAAGAACCCTGAAAAGCCAGACGACTATCTAGTCAGAAGATTAGCTGCGACTGAAGGTTATGAAATGGCTTCTACTGATGAAAAGGACGAATCGTTTGTTCTTGAGAAGGATCAGTGTTGGGTAGTGGCTGAAAATGAGAAATTGAAAGCCAAGGAAGCAATTGACAGTCGAACATTTGGTCCAATTCACATGACCAACATCGTAGGCAGAGTCCTATACTGCCTGCGAAATGCTGTAGACCATGGCCGTGTACAGAACAGTCCTTTCAGTATGCAAAAGGATTCACCAGTGTTGGAAATGGAACTTGATGTTGATGAGATGGTAAAAAGTCACAAAGCCTGA

Protein sequence

>Msa0027580

MVSFTTWCRYLQSKAQYTIDLGYKNHKGGHVKDNELMDFIFKHFTNGKMTFLHWTKGEEMAPTIDAKGSTLLVRRLPIPDQKRVYVGDVVVLKNPEKPDDYLVRRLAATEGYEMASTDEKDESFVLEKDQCWVVAENEKLKAKEAIDSRTFGPIHMTNIVGRVLYCLRNAVDHGRVQNSPFSMQKDSPVLEMELDVDEMVKSHKA*