AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0178010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178010 MtrunA17_Chr1g0200981 98.611 360 5 0 1 360 1 360 0.0 743
Msa0178010 MtrunA17_Chr6g0465111 69.296 355 109 0 6 360 7 361 0.0 537
Msa0178010 MtrunA17_Chr6g0465121 65.915 355 116 1 6 360 20 369 1.19e-180 505
Msa0178010 MtrunA17_Chr6g0464971 61.582 354 136 0 6 359 9 362 1.04e-166 469
Msa0178010 MtrunA17_Chr6g0464961 62.994 354 131 0 6 359 31 384 2.14e-158 451
Msa0178010 MtrunA17_Chr6g0477771 52.355 361 167 2 2 358 3 362 9.00e-139 399
Msa0178010 MtrunA17_Chr3g0110001 49.300 357 177 2 6 358 7 363 9.87e-124 360
Msa0178010 MtrunA17_Chr6g0464931 67.647 102 33 0 6 107 9 110 1.96e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178010 AT4G09670.1 65.266 357 124 0 2 358 3 359 3.80e-179 501
Msa0178010 AT1G34200.1 57.955 352 136 3 6 356 7 347 9.38e-144 411
Msa0178010 AT1G66130.1 39.227 362 207 6 6 358 7 364 1.95e-84 260

Find 100 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGCTCAATTGGCATCTT+TGG 0.150387 1_4:-65383763 Msa0178010:three_prime_UTR
GGATAATGGGGTTCAGTTTA+TGG 0.156726 1_4:-65385510 Msa0178010:CDS
GAAGCAAATGCTTCTTATTC+TGG 0.175761 1_4:+65385591 None:intergenic
TCGCTGCATTATCAAATTAA+AGG 0.182090 1_4:+65386049 None:intergenic
GATCAGTGCTTCTGACATTT+TGG 0.185299 1_4:+65385868 None:intergenic
TAACAAAGATGGAGTTATAT+TGG 0.254546 1_4:-65384250 Msa0178010:CDS
TAATCCTAGGACTGCTAAAA+TGG 0.281705 1_4:-65385468 Msa0178010:CDS
TGGGAGCTTGTGAGGATAAT+GGG 0.292083 1_4:-65385523 Msa0178010:CDS
CGGCGCAGATGGTGGCGTTT+GGG 0.299088 1_4:+65385783 None:intergenic
ACGGCGCAGATGGTGGCGTT+TGG 0.303690 1_4:+65385782 None:intergenic
CTGTTAAGTCTTCAATTCAT+AGG 0.306990 1_4:-65383864 Msa0178010:CDS
TTTATATTATATTGAGAGAT+TGG 0.310543 1_4:-65385962 Msa0178010:five_prime_UTR
GGCGTTTGGGGATAGGTTGA+TGG 0.319210 1_4:+65385796 None:intergenic
GTGGGAGCTTGTGAGGATAA+TGG 0.326462 1_4:-65385524 Msa0178010:CDS
CAAAATGTGATCTGCTCAAT+TGG 0.327124 1_4:-65383772 Msa0178010:three_prime_UTR
GGTTACAGAATTTGCTCGTT+TGG 0.359965 1_4:-65383968 Msa0178010:CDS
TTGATGATCTTGTTACTTGT+TGG 0.364651 1_4:-65384047 Msa0178010:CDS
ACAATGGATATAACAGCTAT+TGG 0.368508 1_4:-65384156 Msa0178010:CDS
GATGCTTCATTCTCTTCATA+AGG 0.382468 1_4:+65384096 None:intergenic
ATGCATTTGTGAGCTGTTCA+TGG 0.388784 1_4:-65383694 Msa0178010:three_prime_UTR
CCTCTGACAGCGAAGGTATA+CGG 0.391217 1_4:-65385707 Msa0178010:CDS
CACTTAAGGTGCAAGCTTGT+TGG 0.397452 1_4:+65385629 None:intergenic
CGGCAACTACGAAGCTCTAC+TGG 0.398318 1_4:-65385687 Msa0178010:CDS
CACAGCCTACTACTCCGATT+TGG 0.398824 1_4:+65385846 None:intergenic
TTCAATGATCCGCACCGCTT+TGG 0.402052 1_4:-65385437 Msa0178010:CDS
CCGCAAGAACGGCCCACTTA+AGG 0.402847 1_4:+65385615 None:intergenic
GGCTTTGTCGTAGTTGCGGC+TGG 0.408541 1_4:+65385757 None:intergenic
ATTGTGGTGCATCACTATAT+TGG 0.411258 1_4:-65384227 Msa0178010:CDS
GTCGTAGTTGCGGCTGGCGA+CGG 0.414698 1_4:+65385763 None:intergenic
GCCGCTTTCGCCACCGCCAA+TGG 0.415224 1_4:-65385734 Msa0178010:CDS
TCTTCCTCAAGAGGCTCTCT+TGG 0.424130 1_4:-65383989 Msa0178010:CDS
AAGCAAATGCTTCTTATTCT+GGG 0.425925 1_4:+65385592 None:intergenic
GAACCATGTGCGTCAAGATC+TGG 0.426777 1_4:+65384369 None:intergenic
AGATGGTGGCGTTTGGGGAT+AGG 0.426809 1_4:+65385789 None:intergenic
CTTCACTCCTCAATCTGAAC+AGG 0.431496 1_4:+65383832 None:intergenic
ACAATTGGCCACTTCTTCTC+AGG 0.432874 1_4:+65383916 None:intergenic
ATAACAGCTATTGGAACAAA+AGG 0.438590 1_4:-65384147 Msa0178010:CDS
CAACAAGCTTGCACCTTAAG+TGG 0.446324 1_4:-65385628 Msa0178010:CDS
TCAGCAATATCCAGCGCAAC+GGG 0.447221 1_4:+65385560 None:intergenic
TGTTAAGTCTTCAATTCATA+GGG 0.452020 1_4:-65383863 Msa0178010:CDS
TTGTGGTGCATCACTATATT+GGG 0.454615 1_4:-65384226 Msa0178010:CDS
ATATACACAGCGTCCACATC+AGG 0.464643 1_4:+65385659 None:intergenic
TCTTGAGAAGCCCGTTGCGC+TGG 0.465138 1_4:-65385570 Msa0178010:CDS
AGGTGCAAGCTTGTTGGTAG+TGG 0.470113 1_4:+65385635 None:intergenic
CTGATCCAAATCGGAGTAGT+AGG 0.471545 1_4:-65385851 Msa0178010:CDS
AGAAGTGGCCAATTGTTAGT+AGG 0.484377 1_4:-65383909 Msa0178010:CDS
ACTCCATCTTTGTTAAGCAC+TGG 0.485256 1_4:+65384258 None:intergenic
GCGGCTGGCGACGGCGCAGA+TGG 0.488870 1_4:+65385772 None:intergenic
TGCTGAGTTTGATGAAATTG+TGG 0.489673 1_4:-65385543 Msa0178010:CDS
CCGCTTTGGTCAACTCAAAT+CGG 0.490151 1_4:-65385423 Msa0178010:intron
GCCATTGGCGGTGGCGAAAG+CGG 0.494977 1_4:+65385733 None:intergenic
ATCTGCAGCAAATGTAAAGC+AGG 0.496037 1_4:+65384425 None:intergenic
CTATGTGGGTTCATAATCCT+AGG 0.500670 1_4:-65385481 Msa0178010:CDS
TACAGAATTTGCTCGTTTGG+TGG 0.508036 1_4:-65383965 Msa0178010:CDS
GCTGGCGACGGCGCAGATGG+TGG 0.508895 1_4:+65385775 None:intergenic
TCAGAAGCACTGATCCAAAT+CGG 0.512386 1_4:-65385860 Msa0178010:CDS
CTTGACGCACATGGTTCTCT+AGG 0.513476 1_4:-65384363 Msa0178010:CDS
CGCTGTCAGAGGGTAGCCAT+TGG 0.518909 1_4:+65385718 None:intergenic
TGTCAGAGGGTAGCCATTGG+CGG 0.521007 1_4:+65385721 None:intergenic
GCTGAGTTTGATGAAATTGT+GGG 0.524578 1_4:-65385542 Msa0178010:CDS
TATAATATAAAATCATTACT+TGG 0.524816 1_4:+65385974 None:intergenic
CCTTAAGTGGGCCGTTCTTG+CGG 0.525534 1_4:-65385615 Msa0178010:CDS
ACAGAATTTGCTCGTTTGGT+GGG 0.526034 1_4:-65383964 Msa0178010:CDS
CATGGTTCTCTAGGTGATGC+AGG 0.528921 1_4:-65384354 Msa0178010:CDS
TTTCTTAGCAAACTTGACAA+TGG 0.530527 1_4:-65384172 Msa0178010:CDS
GGCGCAGATGGTGGCGTTTG+GGG 0.530549 1_4:+65385784 None:intergenic
CAGAGGGTAGCCATTGGCGG+TGG 0.533110 1_4:+65385724 None:intergenic
GTTTATGGATAATACTATGT+GGG 0.534341 1_4:-65385495 Msa0178010:CDS
CCGTATACCTTCGCTGTCAG+AGG 0.534458 1_4:+65385707 None:intergenic
CTTATTCTGGGCCGCAAGAA+CGG 0.540934 1_4:+65385604 None:intergenic
GTTCGTAGTTGGCAGCTAAG+AGG 0.542085 1_4:+65384310 None:intergenic
AGAAACTTTACGAGCTATGT+CGG 0.550870 1_4:+65385823 None:intergenic
CGGCGGCTTTGTCGTAGTTG+CGG 0.552909 1_4:+65385753 None:intergenic
GTTCTCTAGGTGATGCAGGT+TGG 0.556860 1_4:-65384350 Msa0178010:CDS
AGTTTATGGATAATACTATG+TGG 0.557985 1_4:-65385496 Msa0178010:CDS
ACTCTAAGCCTGAGAAGAAG+TGG 0.559686 1_4:-65383924 Msa0178010:CDS
ATTGGCGGTGGCGAAAGCGG+CGG 0.561135 1_4:+65385736 None:intergenic
CTCAGCAATATCCAGCGCAA+CGG 0.568452 1_4:+65385559 None:intergenic
GCATCACTATATTGGGAGGA+TGG 0.568538 1_4:-65384219 Msa0178010:CDS
TAAAACTGATCTTCCTCAAG+AGG 0.573676 1_4:-65383998 Msa0178010:CDS
GAACCAGTGCTTAACAAAGA+TGG 0.577418 1_4:-65384261 Msa0178010:CDS
TGAAATTGTGGGAGCTTGTG+AGG 0.599951 1_4:-65385531 Msa0178010:CDS
CGTATACCTTCGCTGTCAGA+GGG 0.599957 1_4:+65385708 None:intergenic
GGGAGCTTGTGAGGATAATG+GGG 0.604851 1_4:-65385522 Msa0178010:CDS
GAGTGTGGTATCTATGTACT+TGG 0.608899 1_4:+65385932 None:intergenic
AAGCCAGATCTTGACGCACA+TGG 0.611601 1_4:-65384372 Msa0178010:CDS
GTTAAGCACTGGTTCACGTG+AGG 0.613013 1_4:+65384269 None:intergenic
AACAAGCTTGCACCTTAAGT+GGG 0.617418 1_4:-65385627 Msa0178010:CDS
GATGGAGTTATATTGGATTG+TGG 0.629420 1_4:-65384243 Msa0178010:CDS
GTTAAGTCTTCAATTCATAG+GGG 0.633153 1_4:-65383862 Msa0178010:CDS
TTGAGTTGACCAAAGCGGTG+CGG 0.636462 1_4:+65385428 None:intergenic
GTAACCAAGAGAGCCTCTTG+AGG 0.645260 1_4:+65383985 None:intergenic
TCAGATTGAGGAGTGAAGCA+AGG 0.645911 1_4:-65383827 Msa0178010:exon
TGGCTACCCTCTGACAGCGA+AGG 0.653414 1_4:-65385714 Msa0178010:CDS
TAGACTGAGCTGAGTGAGTG+TGG 0.654005 1_4:+65385917 None:intergenic
CCGATTTGAGTTGACCAAAG+CGG 0.657625 1_4:+65385423 None:intergenic
TATACACAGCGTCCACATCA+GGG 0.659580 1_4:+65385660 None:intergenic
TTTGAGAAGATACAGGCATG+TGG 0.695799 1_4:+65383791 None:intergenic
TCTACTGGAAGACCCTGATG+TGG 0.699995 1_4:-65385672 Msa0178010:CDS
TGGTGCATCACTATATTGGG+AGG 0.737696 1_4:-65384223 Msa0178010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATATAAAATCATTACT+TGG + chr1_4:65383897-65383916 None:intergenic 10.0%
!! TTTATATTATATTGAGAGAT+TGG - chr1_4:65383906-65383925 Msa0178010:CDS 15.0%
!! TACTTATTACTATACAAAAA+TGG + chr1_4:65384676-65384695 None:intergenic 15.0%
!! GAAAAAAATAGTGAAAACAA+AGG - chr1_4:65384884-65384903 Msa0178010:intron 20.0%
!!! TAAAATGACATTAAGTTGTT+CGG - chr1_4:65385124-65385143 Msa0178010:intron 20.0%
!!! ATTTTGAAATATTGATAGTG+TGG - chr1_4:65385277-65385296 Msa0178010:intron 20.0%
!!! TTTTGAAATATTGATAGTGT+GGG - chr1_4:65385278-65385297 Msa0178010:intron 20.0%
!! ACTTGTTTACTTGTAAAATT+TGG - chr1_4:65385326-65385345 Msa0178010:intron 20.0%
!! CTTGCAATACAAATTTTTAC+TGG + chr1_4:65383853-65383872 None:intergenic 25.0%
! AGTTTATGGATAATACTATG+TGG - chr1_4:65384372-65384391 Msa0178010:CDS 25.0%
! GTTTATGGATAATACTATGT+GGG - chr1_4:65384373-65384392 Msa0178010:CDS 25.0%
! CACAGCTTAACAAATTTATT+TGG + chr1_4:65384519-65384538 None:intergenic 25.0%
! TAATTTCAAGACTAGTGTAT+GGG + chr1_4:65384567-65384586 None:intergenic 25.0%
! ACTAGAATACTAGATTGATA+TGG + chr1_4:65385030-65385049 None:intergenic 25.0%
!! TTGTTACTTCAAAGATCATT+TGG + chr1_4:65385072-65385091 None:intergenic 25.0%
! TAACAAAGATGGAGTTATAT+TGG - chr1_4:65385618-65385637 Msa0178010:CDS 25.0%
!!! GTTTTAACAGTACACTTAAT+TGG + chr1_4:65385856-65385875 None:intergenic 25.0%
! TGTTAAGTCTTCAATTCATA+GGG - chr1_4:65386005-65386024 Msa0178010:five_prime_UTR 25.0%
TCGCTGCATTATCAAATTAA+AGG + chr1_4:65383822-65383841 None:intergenic 30.0%
! GCAATACAAATTTTTACTGG+AGG + chr1_4:65383850-65383869 None:intergenic 30.0%
AAGCAAATGCTTCTTATTCT+GGG + chr1_4:65384279-65384298 None:intergenic 30.0%
GTAATTTCAAGACTAGTGTA+TGG + chr1_4:65384568-65384587 None:intergenic 30.0%
AGAGAGAAAAGAGAAAATAG+AGG - chr1_4:65384844-65384863 Msa0178010:intron 30.0%
GAGAGAAAAGAGAAAATAGA+GGG - chr1_4:65384845-65384864 Msa0178010:intron 30.0%
ATGGTAGTAAGTACTCAAAT+TGG - chr1_4:65384987-65385006 Msa0178010:intron 30.0%
! TAAGTACTCAAATTGGTTCT+TGG - chr1_4:65384994-65385013 Msa0178010:intron 30.0%
! AAATTGGTTCTTGGATTTGT+AGG - chr1_4:65385003-65385022 Msa0178010:intron 30.0%
! ATTTTCACCACTAGACTTTT+TGG - chr1_4:65385215-65385234 Msa0178010:intron 30.0%
TGCCTATTTACTGATATAAG+TGG - chr1_4:65385394-65385413 Msa0178010:intron 30.0%
CACCACTTATATCAGTAAAT+AGG + chr1_4:65385399-65385418 None:intergenic 30.0%
CTATTTACTGATATAAGTGG+TGG - chr1_4:65385397-65385416 Msa0178010:intron 30.0%
TTTCTTAGCAAACTTGACAA+TGG - chr1_4:65385696-65385715 Msa0178010:CDS 30.0%
ACAATGGATATAACAGCTAT+TGG - chr1_4:65385712-65385731 Msa0178010:CDS 30.0%
ATAACAGCTATTGGAACAAA+AGG - chr1_4:65385721-65385740 Msa0178010:CDS 30.0%
!! AATGAAGCATCTTTTCATGT+TGG - chr1_4:65385784-65385803 Msa0178010:CDS 30.0%
! TTGATGATCTTGTTACTTGT+TGG - chr1_4:65385821-65385840 Msa0178010:CDS 30.0%
! TGTGTTTTCCTACTAACAAT+TGG + chr1_4:65385970-65385989 None:intergenic 30.0%
CTGTTAAGTCTTCAATTCAT+AGG - chr1_4:65386004-65386023 Msa0178010:five_prime_UTR 30.0%
GTTAAGTCTTCAATTCATAG+GGG - chr1_4:65386006-65386025 Msa0178010:five_prime_UTR 30.0%
!! ATCACATTTTGAGAAGATAC+AGG + chr1_4:65386087-65386106 None:intergenic 30.0%
AGAAACTTTACGAGCTATGT+CGG + chr1_4:65384048-65384067 None:intergenic 35.0%
GAAGCAAATGCTTCTTATTC+TGG + chr1_4:65384280-65384299 None:intergenic 35.0%
TGCTGAGTTTGATGAAATTG+TGG - chr1_4:65384325-65384344 Msa0178010:CDS 35.0%
GCTGAGTTTGATGAAATTGT+GGG - chr1_4:65384326-65384345 Msa0178010:CDS 35.0%
TAATCCTAGGACTGCTAAAA+TGG - chr1_4:65384400-65384419 Msa0178010:CDS 35.0%
CCAAAAATAACAGTAGAGCT+AGG + chr1_4:65384492-65384511 None:intergenic 35.0%
!!! CCTAGCTCTACTGTTATTTT+TGG - chr1_4:65384489-65384508 Msa0178010:intron 35.0%
TAAGACTTTCAGACTTGAGA+AGG + chr1_4:65384543-65384562 None:intergenic 35.0%
TGTAGTCAAAGTCAAGATGT+AGG - chr1_4:65384739-65384758 Msa0178010:intron 35.0%
TCAAAGTCAAGATGTAGGAT+TGG - chr1_4:65384744-65384763 Msa0178010:intron 35.0%
TCATGTTTACGTTCCTTCAT+TGG + chr1_4:65384774-65384793 None:intergenic 35.0%
AATGAAGGAACGTAAACATG+AGG - chr1_4:65384773-65384792 Msa0178010:intron 35.0%
ACGTAACACAATTCGTAAGT+AGG - chr1_4:65384797-65384816 Msa0178010:intron 35.0%
ACACAATTCGTAAGTAGGAT+CGG - chr1_4:65384802-65384821 Msa0178010:intron 35.0%
TTGTAGTAAAGATCGAGTGT+TGG + chr1_4:65384968-65384987 None:intergenic 35.0%
ACACTCGATCTTTACTACAA+TGG - chr1_4:65384968-65384987 Msa0178010:intron 35.0%
AATCAAATTTGTCGCTCTGT+TGG - chr1_4:65385098-65385117 Msa0178010:intron 35.0%
ATCAAATTTGTCGCTCTGTT+GGG - chr1_4:65385099-65385118 Msa0178010:intron 35.0%
GACTGCAAAGTTTGATATGT+TGG - chr1_4:65385170-65385189 Msa0178010:intron 35.0%
ACTTTGTCCAAAAAGTCTAG+TGG + chr1_4:65385225-65385244 None:intergenic 35.0%
!! ATTGATAGTGTGGGAGATTA+AGG - chr1_4:65385287-65385306 Msa0178010:intron 35.0%
GATGGAGTTATATTGGATTG+TGG - chr1_4:65385625-65385644 Msa0178010:CDS 35.0%
ATTGTGGTGCATCACTATAT+TGG - chr1_4:65385641-65385660 Msa0178010:CDS 35.0%
TTGTGGTGCATCACTATATT+GGG - chr1_4:65385642-65385661 Msa0178010:CDS 35.0%
GATGCTTCATTCTCTTCATA+AGG + chr1_4:65385775-65385794 None:intergenic 35.0%
TAAAACTGATCTTCCTCAAG+AGG - chr1_4:65385870-65385889 Msa0178010:exon 35.0%
CAAAATGTGATCTGCTCAAT+TGG - chr1_4:65386096-65386115 Msa0178010:five_prime_UTR 35.0%
GAGTGTGGTATCTATGTACT+TGG + chr1_4:65383939-65383958 None:intergenic 40.0%
! GATCAGTGCTTCTGACATTT+TGG + chr1_4:65384003-65384022 None:intergenic 40.0%
!! TCAGAAGCACTGATCCAAAT+CGG - chr1_4:65384008-65384027 Msa0178010:CDS 40.0%
AACAAGCTTGCACCTTAAGT+GGG - chr1_4:65384241-65384260 Msa0178010:CDS 40.0%
!! GGATAATGGGGTTCAGTTTA+TGG - chr1_4:65384358-65384377 Msa0178010:CDS 40.0%
CTATGTGGGTTCATAATCCT+AGG - chr1_4:65384387-65384406 Msa0178010:CDS 40.0%
! AGTCAAGATGTAGGATTGGT+CGG - chr1_4:65384748-65384767 Msa0178010:intron 40.0%
AATTCGTAAGTAGGATCGGT+AGG - chr1_4:65384806-65384825 Msa0178010:intron 40.0%
CAGAGCGACAAATTTGATTG+AGG + chr1_4:65385097-65385116 None:intergenic 40.0%
CTGATATAAGTGGTGGCTTT+CGG - chr1_4:65385404-65385423 Msa0178010:intron 40.0%
TGATATAAGTGGTGGCTTTC+GGG - chr1_4:65385405-65385424 Msa0178010:intron 40.0%
ATCTGCAGCAAATGTAAAGC+AGG + chr1_4:65385446-65385465 None:intergenic 40.0%
!!! TGTTTTAGGGAGTTCGTAGT+TGG + chr1_4:65385572-65385591 None:intergenic 40.0%
! GTGAGGCTATCACTGTTTTA+GGG + chr1_4:65385585-65385604 None:intergenic 40.0%
GAACCAGTGCTTAACAAAGA+TGG - chr1_4:65385607-65385626 Msa0178010:CDS 40.0%
ACTCCATCTTTGTTAAGCAC+TGG + chr1_4:65385613-65385632 None:intergenic 40.0%
! GGTTACAGAATTTGCTCGTT+TGG - chr1_4:65385900-65385919 Msa0178010:five_prime_UTR 40.0%
! TACAGAATTTGCTCGTTTGG+TGG - chr1_4:65385903-65385922 Msa0178010:five_prime_UTR 40.0%
! ACAGAATTTGCTCGTTTGGT+GGG - chr1_4:65385904-65385923 Msa0178010:five_prime_UTR 40.0%
AGAAGTGGCCAATTGTTAGT+AGG - chr1_4:65385959-65385978 Msa0178010:five_prime_UTR 40.0%
! TTTTGAGCCTGTTCAGATTG+AGG - chr1_4:65386029-65386048 Msa0178010:five_prime_UTR 40.0%
TTTGAGAAGATACAGGCATG+TGG + chr1_4:65386080-65386099 None:intergenic 40.0%
ATCTGCTCAATTGGCATCTT+TGG - chr1_4:65386105-65386124 Msa0178010:five_prime_UTR 40.0%
ATGCATTTGTGAGCTGTTCA+TGG - chr1_4:65386174-65386193 Msa0178010:five_prime_UTR 40.0%
AAGATAGAAGTCCCCCTACA+AGG + chr1_4:65383652-65383671 None:intergenic 45.0%
CTGATCCAAATCGGAGTAGT+AGG - chr1_4:65384017-65384036 Msa0178010:CDS 45.0%
TATACACAGCGTCCACATCA+GGG + chr1_4:65384211-65384230 None:intergenic 45.0%
ATATACACAGCGTCCACATC+AGG + chr1_4:65384212-65384231 None:intergenic 45.0%
CACTTAAGGTGCAAGCTTGT+TGG + chr1_4:65384242-65384261 None:intergenic 45.0%
CAACAAGCTTGCACCTTAAG+TGG - chr1_4:65384240-65384259 Msa0178010:CDS 45.0%
TGAAATTGTGGGAGCTTGTG+AGG - chr1_4:65384337-65384356 Msa0178010:CDS 45.0%
TGGGAGCTTGTGAGGATAAT+GGG - chr1_4:65384345-65384364 Msa0178010:CDS 45.0%
!!! TGTGCCATTTTAGCAGTCCT+AGG + chr1_4:65384407-65384426 None:intergenic 45.0%
!! CCGATTTGAGTTGACCAAAG+CGG + chr1_4:65384448-65384467 None:intergenic 45.0%
CCGCTTTGGTCAACTCAAAT+CGG - chr1_4:65384445-65384464 Msa0178010:intron 45.0%
TAGGCAATGAACATGCAGTC+AGG + chr1_4:65385380-65385399 None:intergenic 45.0%
! CGTGAGGCTATCACTGTTTT+AGG + chr1_4:65385586-65385605 None:intergenic 45.0%
TGGTGCATCACTATATTGGG+AGG - chr1_4:65385645-65385664 Msa0178010:CDS 45.0%
GCATCACTATATTGGGAGGA+TGG - chr1_4:65385649-65385668 Msa0178010:CDS 45.0%
ACTCTAAGCCTGAGAAGAAG+TGG - chr1_4:65385944-65385963 Msa0178010:five_prime_UTR 45.0%
ACAATTGGCCACTTCTTCTC+AGG + chr1_4:65385955-65385974 None:intergenic 45.0%
CTTCACTCCTCAATCTGAAC+AGG + chr1_4:65386039-65386058 None:intergenic 45.0%
TCAGATTGAGGAGTGAAGCA+AGG - chr1_4:65386041-65386060 Msa0178010:five_prime_UTR 45.0%
TAGACTGAGCTGAGTGAGTG+TGG + chr1_4:65383954-65383973 None:intergenic 50.0%
CACAGCCTACTACTCCGATT+TGG + chr1_4:65384025-65384044 None:intergenic 50.0%
CGTATACCTTCGCTGTCAGA+GGG + chr1_4:65384163-65384182 None:intergenic 50.0%
! CCTCTGACAGCGAAGGTATA+CGG - chr1_4:65384161-65384180 Msa0178010:CDS 50.0%
TCTACTGGAAGACCCTGATG+TGG - chr1_4:65384196-65384215 Msa0178010:CDS 50.0%
!! AGGTGCAAGCTTGTTGGTAG+TGG + chr1_4:65384236-65384255 None:intergenic 50.0%
CTTATTCTGGGCCGCAAGAA+CGG + chr1_4:65384267-65384286 None:intergenic 50.0%
TCAGCAATATCCAGCGCAAC+GGG + chr1_4:65384311-65384330 None:intergenic 50.0%
CTCAGCAATATCCAGCGCAA+CGG + chr1_4:65384312-65384331 None:intergenic 50.0%
GTGGGAGCTTGTGAGGATAA+TGG - chr1_4:65384344-65384363 Msa0178010:CDS 50.0%
GGGAGCTTGTGAGGATAATG+GGG - chr1_4:65384346-65384365 Msa0178010:CDS 50.0%
TTCAATGATCCGCACCGCTT+TGG - chr1_4:65384431-65384450 Msa0178010:CDS 50.0%
!! TTGAGTTGACCAAAGCGGTG+CGG + chr1_4:65384443-65384462 None:intergenic 50.0%
!! TAGGATTGGTCGGCCAATGA+AGG - chr1_4:65384758-65384777 Msa0178010:intron 50.0%
GTAACATCGAAGCACGGTTG+AGG - chr1_4:65384921-65384940 Msa0178010:intron 50.0%
!! AAGCACGGTTGAGGTGTTGT+GGG - chr1_4:65384930-65384949 Msa0178010:intron 50.0%
AAGCCAGATCTTGACGCACA+TGG - chr1_4:65385496-65385515 Msa0178010:CDS 50.0%
GAACCATGTGCGTCAAGATC+TGG + chr1_4:65385502-65385521 None:intergenic 50.0%
CTTGACGCACATGGTTCTCT+AGG - chr1_4:65385505-65385524 Msa0178010:CDS 50.0%
CATGGTTCTCTAGGTGATGC+AGG - chr1_4:65385514-65385533 Msa0178010:CDS 50.0%
GTTCTCTAGGTGATGCAGGT+TGG - chr1_4:65385518-65385537 Msa0178010:CDS 50.0%
GTTCGTAGTTGGCAGCTAAG+AGG + chr1_4:65385561-65385580 None:intergenic 50.0%
!! GTTAAGCACTGGTTCACGTG+AGG + chr1_4:65385602-65385621 None:intergenic 50.0%
TCTTCCTCAAGAGGCTCTCT+TGG - chr1_4:65385879-65385898 Msa0178010:exon 50.0%
GTAACCAAGAGAGCCTCTTG+AGG + chr1_4:65385886-65385905 None:intergenic 50.0%
! GGCGTTTGGGGATAGGTTGA+TGG + chr1_4:65384075-65384094 None:intergenic 55.0%
! AGATGGTGGCGTTTGGGGAT+AGG + chr1_4:65384082-65384101 None:intergenic 55.0%
TGTCAGAGGGTAGCCATTGG+CGG + chr1_4:65384150-65384169 None:intergenic 55.0%
CCGTATACCTTCGCTGTCAG+AGG + chr1_4:65384164-65384183 None:intergenic 55.0%
CGGCAACTACGAAGCTCTAC+TGG - chr1_4:65384181-65384200 Msa0178010:CDS 55.0%
CCTTAAGTGGGCCGTTCTTG+CGG - chr1_4:65384253-65384272 Msa0178010:CDS 55.0%
CCGTGCTTCGATGTTACGCA+GGG + chr1_4:65384918-65384937 None:intergenic 55.0%
CCCTGCGTAACATCGAAGCA+CGG - chr1_4:65384915-65384934 Msa0178010:intron 55.0%
ACCGTGCTTCGATGTTACGC+AGG + chr1_4:65384919-65384938 None:intergenic 55.0%
!! GAAGCACGGTTGAGGTGTTG+TGG - chr1_4:65384929-65384948 Msa0178010:intron 55.0%
! GGCTTTGTCGTAGTTGCGGC+TGG + chr1_4:65384114-65384133 None:intergenic 60.0%
! CGGCGGCTTTGTCGTAGTTG+CGG + chr1_4:65384118-65384137 None:intergenic 60.0%
CGCTGTCAGAGGGTAGCCAT+TGG + chr1_4:65384153-65384172 None:intergenic 60.0%
TGGCTACCCTCTGACAGCGA+AGG - chr1_4:65384154-65384173 Msa0178010:CDS 60.0%
CCGCAAGAACGGCCCACTTA+AGG + chr1_4:65384256-65384275 None:intergenic 60.0%
TCTTGAGAAGCCCGTTGCGC+TGG - chr1_4:65384298-65384317 Msa0178010:CDS 60.0%
TGGTGGCTTTCGGGCTGTAC+AGG - chr1_4:65385414-65385433 Msa0178010:intron 60.0%
! GGCGCAGATGGTGGCGTTTG+GGG + chr1_4:65384087-65384106 None:intergenic 65.0%
! CGGCGCAGATGGTGGCGTTT+GGG + chr1_4:65384088-65384107 None:intergenic 65.0%
! ACGGCGCAGATGGTGGCGTT+TGG + chr1_4:65384089-65384108 None:intergenic 65.0%
GTCGTAGTTGCGGCTGGCGA+CGG + chr1_4:65384108-65384127 None:intergenic 65.0%
ATTGGCGGTGGCGAAAGCGG+CGG + chr1_4:65384135-65384154 None:intergenic 65.0%
GCCATTGGCGGTGGCGAAAG+CGG + chr1_4:65384138-65384157 None:intergenic 65.0%
CAGAGGGTAGCCATTGGCGG+TGG + chr1_4:65384147-65384166 None:intergenic 65.0%
! GCCGCTTTCGCCACCGCCAA+TGG - chr1_4:65384134-65384153 Msa0178010:CDS 70.0%
GCTGGCGACGGCGCAGATGG+TGG + chr1_4:65384096-65384115 None:intergenic 75.0%
! GCGGCTGGCGACGGCGCAGA+TGG + chr1_4:65384099-65384118 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 65383640 65386250 65383640 ID=Msa0178010;Name=Msa0178010
chr1_4 mRNA 65383640 65386250 65383640 ID=Msa0178010-mRNA-1;Parent=Msa0178010;Name=Msa0178010-mRNA-1;_AED=0.02;_eAED=0.02;_QI=365|1|1|1|1|1|2|194|360
chr1_4 exon 65383640 65384454 65383640 ID=Msa0178010-mRNA-1:exon:21218;Parent=Msa0178010-mRNA-1
chr1_4 exon 65385424 65386250 65385424 ID=Msa0178010-mRNA-1:exon:21217;Parent=Msa0178010-mRNA-1
chr1_4 five_prime_UTR 65385886 65386250 65385886 ID=Msa0178010-mRNA-1:five_prime_utr;Parent=Msa0178010-mRNA-1
chr1_4 CDS 65385424 65385885 65385424 ID=Msa0178010-mRNA-1:cds;Parent=Msa0178010-mRNA-1
chr1_4 CDS 65383834 65384454 65383834 ID=Msa0178010-mRNA-1:cds;Parent=Msa0178010-mRNA-1
chr1_4 three_prime_UTR 65383640 65383833 65383640 ID=Msa0178010-mRNA-1:three_prime_utr;Parent=Msa0178010-mRNA-1
Gene Sequence

>Msa0178010

ATGTCAGAAGCACTGATCCAAATCGGAGTAGTAGGCTGTGCCGACATAGCTCGTAAAGTTTCTCGCGCCATCAACCTATCCCCAAACGCCACCATCTGCGCCGTCGCCAGCCGCAACTACGACAAAGCCGCCGCTTTCGCCACCGCCAATGGCTACCCTCTGACAGCGAAGGTATACGGCAACTACGAAGCTCTACTGGAAGACCCTGATGTGGACGCTGTGTATATGCCACTACCAACAAGCTTGCACCTTAAGTGGGCCGTTCTTGCGGCCCAGAATAAGAAGCATTTGCTTCTTGAGAAGCCCGTTGCGCTGGATATTGCTGAGTTTGATGAAATTGTGGGAGCTTGTGAGGATAATGGGGTTCAGTTTATGGATAATACTATGTGGGTTCATAATCCTAGGACTGCTAAAATGGCACAGTTCTTCAATGATCCGCACCGCTTTGGTCAACTCAAATCGGTTCGCACCTGCTTTACATTTGCTGCAGATTCTGAATATCTAAAGAAAGACATTCGTGTGAAGCCAGATCTTGACGCACATGGTTCTCTAGGTGATGCAGGTTGGTATTGCATTCGTGCAATCCTCTTAGCTGCCAACTACGAACTCCCTAAAACAGTGATAGCCTCACGTGAACCAGTGCTTAACAAAGATGGAGTTATATTGGATTGTGGTGCATCACTATATTGGGAGGATGGTAAAGTAGCTACATTTCATTGCTCTTTCTTAGCAAACTTGACAATGGATATAACAGCTATTGGAACAAAAGGAACTCTTCATGTTCATGATTTTATTATTCCTTATGAAGAGAATGAAGCATCTTTTCATGTTGGTACTGAAACTAGCTTTGATGATCTTGTTACTTGTTGGTCTAAACAGCCAATTAAGTGTACTGTTAAAACTGATCTTCCTCAAGAGGCTCTCTTGGTTACAGAATTTGCTCGTTTGGTGGGTGAAATTAAATTTAGAAACTCTAAGCCTGAGAAGAAGTGGCCAATTGTTAGTAGGAAAACACAACTTGTGTTGAATGCTGTTAAGTCTTCAATTCATAGGGGTTTTGAGCCTGTTCAGATTGAGGAGTGA
Protein sequence

>Msa0178010

MSEALIQIGVVGCADIARKVSRAINLSPNATICAVASRNYDKAAAFATANGYPLTAKVYGNYEALLEDPDVDAVYMPLPTSLHLKWAVLAAQNKKHLLLEKPVALDIAEFDEIVGACEDNGVQFMDNTMWVHNPRTAKMAQFFNDPHRFGQLKSVRTCFTFAADSEYLKKDIRVKPDLDAHGSLGDAGWYCIRAILLAANYELPKTVIASREPVLNKDGVILDCGASLYWEDGKVATFHCSFLANLTMDITAIGTKGTLHVHDFIIPYEENEASFHVGTETSFDDLVTCWSKQPIKCTVKTDLPQEALLVTEFARLVGEIKFRNSKPEKKWPIVSRKTQLVLNAVKSSIHRGFEPVQIEE*