AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1439870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439870 MtrunA17_Chr1g0200981 98.611 360 5 0 1 360 1 360 0.0 743
Msa1439870 MtrunA17_Chr6g0465111 69.296 355 109 0 6 360 7 361 0.0 537
Msa1439870 MtrunA17_Chr6g0465121 65.915 355 116 1 6 360 20 369 1.19e-180 505
Msa1439870 MtrunA17_Chr6g0464971 61.582 354 136 0 6 359 9 362 1.04e-166 469
Msa1439870 MtrunA17_Chr6g0464961 62.994 354 131 0 6 359 31 384 2.14e-158 451
Msa1439870 MtrunA17_Chr6g0477771 52.355 361 167 2 2 358 3 362 9.00e-139 399
Msa1439870 MtrunA17_Chr3g0110001 49.300 357 177 2 6 358 7 363 9.87e-124 360
Msa1439870 MtrunA17_Chr6g0464931 67.647 102 33 0 6 107 9 110 1.96e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439870 AT4G09670.1 65.266 357 124 0 2 358 3 359 3.80e-179 501
Msa1439870 AT1G34200.1 57.955 352 136 3 6 356 7 347 9.38e-144 411
Msa1439870 AT1G66130.1 39.227 362 207 6 6 358 7 364 1.95e-84 260

Find 100 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGCTCAATTGGCATCTT+TGG 0.150387 tig0028900:-33346 Msa1439870:three_prime_UTR
GGATAATGGGGTTCAGTTTA+TGG 0.156726 tig0028900:-35093 Msa1439870:CDS
GAAGCAAATGCTTCTTATTC+TGG 0.175761 tig0028900:+35174 None:intergenic
TCGCTGCATTATCAAATTAA+AGG 0.182090 tig0028900:+35632 None:intergenic
GATCAGTGCTTCTGACATTT+TGG 0.185299 tig0028900:+35451 None:intergenic
TAACAAAGATGGAGTTATAT+TGG 0.254546 tig0028900:-33833 Msa1439870:CDS
TAATCCTAGGACTGCTAAAA+TGG 0.281705 tig0028900:-35051 Msa1439870:CDS
TGGGAGCTTGTGAGGATAAT+GGG 0.292083 tig0028900:-35106 Msa1439870:CDS
CGGCGCAGATGGTGGCGTTT+GGG 0.299088 tig0028900:+35366 None:intergenic
ACGGCGCAGATGGTGGCGTT+TGG 0.303690 tig0028900:+35365 None:intergenic
CTGTTAAGTCTTCAATTCAT+AGG 0.306990 tig0028900:-33447 Msa1439870:CDS
TTTATATTATATTGAGAGAT+TGG 0.310543 tig0028900:-35545 Msa1439870:five_prime_UTR
GGCGTTTGGGGATAGGTTGA+TGG 0.319210 tig0028900:+35379 None:intergenic
GTGGGAGCTTGTGAGGATAA+TGG 0.326462 tig0028900:-35107 Msa1439870:CDS
CAAAATGTGATCTGCTCAAT+TGG 0.327124 tig0028900:-33355 Msa1439870:three_prime_UTR
GGTTACAGAATTTGCTCGTT+TGG 0.359965 tig0028900:-33551 Msa1439870:CDS
TTGATGATCTTGTTACTTGT+TGG 0.364651 tig0028900:-33630 Msa1439870:CDS
ACAATGGATATAACAGCTAT+TGG 0.368508 tig0028900:-33739 Msa1439870:CDS
GATGCTTCATTCTCTTCATA+AGG 0.382468 tig0028900:+33679 None:intergenic
ATGCATTTGTGAGCTGTTCA+TGG 0.388784 tig0028900:-33277 Msa1439870:three_prime_UTR
CCTCTGACAGCGAAGGTATA+CGG 0.391217 tig0028900:-35290 Msa1439870:CDS
CACTTAAGGTGCAAGCTTGT+TGG 0.397452 tig0028900:+35212 None:intergenic
CGGCAACTACGAAGCTCTAC+TGG 0.398318 tig0028900:-35270 Msa1439870:CDS
CACAGCCTACTACTCCGATT+TGG 0.398824 tig0028900:+35429 None:intergenic
TTCAATGATCCGCACCGCTT+TGG 0.402052 tig0028900:-35020 Msa1439870:CDS
CCGCAAGAACGGCCCACTTA+AGG 0.402847 tig0028900:+35198 None:intergenic
GGCTTTGTCGTAGTTGCGGC+TGG 0.408541 tig0028900:+35340 None:intergenic
ATTGTGGTGCATCACTATAT+TGG 0.411258 tig0028900:-33810 Msa1439870:CDS
GTCGTAGTTGCGGCTGGCGA+CGG 0.414698 tig0028900:+35346 None:intergenic
GCCGCTTTCGCCACCGCCAA+TGG 0.415224 tig0028900:-35317 Msa1439870:CDS
TCTTCCTCAAGAGGCTCTCT+TGG 0.424130 tig0028900:-33572 Msa1439870:CDS
AAGCAAATGCTTCTTATTCT+GGG 0.425925 tig0028900:+35175 None:intergenic
GAACCATGTGCGTCAAGATC+TGG 0.426777 tig0028900:+33952 None:intergenic
AGATGGTGGCGTTTGGGGAT+AGG 0.426809 tig0028900:+35372 None:intergenic
CTTCACTCCTCAATCTGAAC+AGG 0.431496 tig0028900:+33415 None:intergenic
ACAATTGGCCACTTCTTCTC+AGG 0.432874 tig0028900:+33499 None:intergenic
ATAACAGCTATTGGAACAAA+AGG 0.438590 tig0028900:-33730 Msa1439870:CDS
CAACAAGCTTGCACCTTAAG+TGG 0.446324 tig0028900:-35211 Msa1439870:CDS
TCAGCAATATCCAGCGCAAC+GGG 0.447221 tig0028900:+35143 None:intergenic
TGTTAAGTCTTCAATTCATA+GGG 0.452020 tig0028900:-33446 Msa1439870:CDS
TTGTGGTGCATCACTATATT+GGG 0.454615 tig0028900:-33809 Msa1439870:CDS
ATATACACAGCGTCCACATC+AGG 0.464643 tig0028900:+35242 None:intergenic
TCTTGAGAAGCCCGTTGCGC+TGG 0.465138 tig0028900:-35153 Msa1439870:CDS
AGGTGCAAGCTTGTTGGTAG+TGG 0.470113 tig0028900:+35218 None:intergenic
CTGATCCAAATCGGAGTAGT+AGG 0.471545 tig0028900:-35434 Msa1439870:CDS
AGAAGTGGCCAATTGTTAGT+AGG 0.484377 tig0028900:-33492 Msa1439870:CDS
ACTCCATCTTTGTTAAGCAC+TGG 0.485256 tig0028900:+33841 None:intergenic
GCGGCTGGCGACGGCGCAGA+TGG 0.488870 tig0028900:+35355 None:intergenic
TGCTGAGTTTGATGAAATTG+TGG 0.489673 tig0028900:-35126 Msa1439870:CDS
CCGCTTTGGTCAACTCAAAT+CGG 0.490151 tig0028900:-35006 Msa1439870:intron
GCCATTGGCGGTGGCGAAAG+CGG 0.494977 tig0028900:+35316 None:intergenic
ATCTGCAGCAAATGTAAAGC+AGG 0.496037 tig0028900:+34008 None:intergenic
CTATGTGGGTTCATAATCCT+AGG 0.500670 tig0028900:-35064 Msa1439870:CDS
TACAGAATTTGCTCGTTTGG+TGG 0.508036 tig0028900:-33548 Msa1439870:CDS
GCTGGCGACGGCGCAGATGG+TGG 0.508895 tig0028900:+35358 None:intergenic
TCAGAAGCACTGATCCAAAT+CGG 0.512386 tig0028900:-35443 Msa1439870:CDS
CTTGACGCACATGGTTCTCT+AGG 0.513476 tig0028900:-33946 Msa1439870:CDS
CGCTGTCAGAGGGTAGCCAT+TGG 0.518909 tig0028900:+35301 None:intergenic
TGTCAGAGGGTAGCCATTGG+CGG 0.521007 tig0028900:+35304 None:intergenic
GCTGAGTTTGATGAAATTGT+GGG 0.524578 tig0028900:-35125 Msa1439870:CDS
TATAATATAAAATCATTACT+TGG 0.524816 tig0028900:+35557 None:intergenic
CCTTAAGTGGGCCGTTCTTG+CGG 0.525534 tig0028900:-35198 Msa1439870:CDS
ACAGAATTTGCTCGTTTGGT+GGG 0.526034 tig0028900:-33547 Msa1439870:CDS
CATGGTTCTCTAGGTGATGC+AGG 0.528921 tig0028900:-33937 Msa1439870:CDS
TTTCTTAGCAAACTTGACAA+TGG 0.530527 tig0028900:-33755 Msa1439870:CDS
GGCGCAGATGGTGGCGTTTG+GGG 0.530549 tig0028900:+35367 None:intergenic
CAGAGGGTAGCCATTGGCGG+TGG 0.533110 tig0028900:+35307 None:intergenic
GTTTATGGATAATACTATGT+GGG 0.534341 tig0028900:-35078 Msa1439870:CDS
CCGTATACCTTCGCTGTCAG+AGG 0.534458 tig0028900:+35290 None:intergenic
CTTATTCTGGGCCGCAAGAA+CGG 0.540934 tig0028900:+35187 None:intergenic
GTTCGTAGTTGGCAGCTAAG+AGG 0.542085 tig0028900:+33893 None:intergenic
AGAAACTTTACGAGCTATGT+CGG 0.550870 tig0028900:+35406 None:intergenic
CGGCGGCTTTGTCGTAGTTG+CGG 0.552909 tig0028900:+35336 None:intergenic
GTTCTCTAGGTGATGCAGGT+TGG 0.556860 tig0028900:-33933 Msa1439870:CDS
AGTTTATGGATAATACTATG+TGG 0.557985 tig0028900:-35079 Msa1439870:CDS
ACTCTAAGCCTGAGAAGAAG+TGG 0.559686 tig0028900:-33507 Msa1439870:CDS
ATTGGCGGTGGCGAAAGCGG+CGG 0.561135 tig0028900:+35319 None:intergenic
CTCAGCAATATCCAGCGCAA+CGG 0.568452 tig0028900:+35142 None:intergenic
GCATCACTATATTGGGAGGA+TGG 0.568538 tig0028900:-33802 Msa1439870:CDS
TAAAACTGATCTTCCTCAAG+AGG 0.573676 tig0028900:-33581 Msa1439870:CDS
GAACCAGTGCTTAACAAAGA+TGG 0.577418 tig0028900:-33844 Msa1439870:CDS
TGAAATTGTGGGAGCTTGTG+AGG 0.599951 tig0028900:-35114 Msa1439870:CDS
CGTATACCTTCGCTGTCAGA+GGG 0.599957 tig0028900:+35291 None:intergenic
GGGAGCTTGTGAGGATAATG+GGG 0.604851 tig0028900:-35105 Msa1439870:CDS
GAGTGTGGTATCTATGTACT+TGG 0.608899 tig0028900:+35515 None:intergenic
AAGCCAGATCTTGACGCACA+TGG 0.611601 tig0028900:-33955 Msa1439870:CDS
GTTAAGCACTGGTTCACGTG+AGG 0.613013 tig0028900:+33852 None:intergenic
AACAAGCTTGCACCTTAAGT+GGG 0.617418 tig0028900:-35210 Msa1439870:CDS
GATGGAGTTATATTGGATTG+TGG 0.629420 tig0028900:-33826 Msa1439870:CDS
GTTAAGTCTTCAATTCATAG+GGG 0.633153 tig0028900:-33445 Msa1439870:CDS
TTGAGTTGACCAAAGCGGTG+CGG 0.636462 tig0028900:+35011 None:intergenic
GTAACCAAGAGAGCCTCTTG+AGG 0.645260 tig0028900:+33568 None:intergenic
TCAGATTGAGGAGTGAAGCA+AGG 0.645911 tig0028900:-33410 Msa1439870:exon
TGGCTACCCTCTGACAGCGA+AGG 0.653414 tig0028900:-35297 Msa1439870:CDS
TAGACTGAGCTGAGTGAGTG+TGG 0.654005 tig0028900:+35500 None:intergenic
CCGATTTGAGTTGACCAAAG+CGG 0.657625 tig0028900:+35006 None:intergenic
TATACACAGCGTCCACATCA+GGG 0.659580 tig0028900:+35243 None:intergenic
TTTGAGAAGATACAGGCATG+TGG 0.695799 tig0028900:+33374 None:intergenic
TCTACTGGAAGACCCTGATG+TGG 0.699995 tig0028900:-35255 Msa1439870:CDS
TGGTGCATCACTATATTGGG+AGG 0.737696 tig0028900:-33806 Msa1439870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATATAAAATCATTACT+TGG + tig0028900:33480-33499 None:intergenic 10.0%
!! TTTATATTATATTGAGAGAT+TGG - tig0028900:33489-33508 Msa1439870:CDS 15.0%
!! TACTTATTACTATACAAAAA+TGG + tig0028900:34259-34278 None:intergenic 15.0%
!! GAAAAAAATAGTGAAAACAA+AGG - tig0028900:34467-34486 Msa1439870:intron 20.0%
!!! TAAAATGACATTAAGTTGTT+CGG - tig0028900:34707-34726 Msa1439870:intron 20.0%
!!! ATTTTGAAATATTGATAGTG+TGG - tig0028900:34860-34879 Msa1439870:intron 20.0%
!!! TTTTGAAATATTGATAGTGT+GGG - tig0028900:34861-34880 Msa1439870:intron 20.0%
!! ACTTGTTTACTTGTAAAATT+TGG - tig0028900:34909-34928 Msa1439870:intron 20.0%
!! CTTGCAATACAAATTTTTAC+TGG + tig0028900:33436-33455 None:intergenic 25.0%
! AGTTTATGGATAATACTATG+TGG - tig0028900:33955-33974 Msa1439870:CDS 25.0%
! GTTTATGGATAATACTATGT+GGG - tig0028900:33956-33975 Msa1439870:CDS 25.0%
! CACAGCTTAACAAATTTATT+TGG + tig0028900:34102-34121 None:intergenic 25.0%
! TAATTTCAAGACTAGTGTAT+GGG + tig0028900:34150-34169 None:intergenic 25.0%
! ACTAGAATACTAGATTGATA+TGG + tig0028900:34613-34632 None:intergenic 25.0%
!! TTGTTACTTCAAAGATCATT+TGG + tig0028900:34655-34674 None:intergenic 25.0%
! TAACAAAGATGGAGTTATAT+TGG - tig0028900:35201-35220 Msa1439870:CDS 25.0%
!!! GTTTTAACAGTACACTTAAT+TGG + tig0028900:35439-35458 None:intergenic 25.0%
! TGTTAAGTCTTCAATTCATA+GGG - tig0028900:35588-35607 Msa1439870:five_prime_UTR 25.0%
TCGCTGCATTATCAAATTAA+AGG + tig0028900:33405-33424 None:intergenic 30.0%
! GCAATACAAATTTTTACTGG+AGG + tig0028900:33433-33452 None:intergenic 30.0%
AAGCAAATGCTTCTTATTCT+GGG + tig0028900:33862-33881 None:intergenic 30.0%
GTAATTTCAAGACTAGTGTA+TGG + tig0028900:34151-34170 None:intergenic 30.0%
AGAGAGAAAAGAGAAAATAG+AGG - tig0028900:34427-34446 Msa1439870:intron 30.0%
GAGAGAAAAGAGAAAATAGA+GGG - tig0028900:34428-34447 Msa1439870:intron 30.0%
ATGGTAGTAAGTACTCAAAT+TGG - tig0028900:34570-34589 Msa1439870:intron 30.0%
! TAAGTACTCAAATTGGTTCT+TGG - tig0028900:34577-34596 Msa1439870:intron 30.0%
! AAATTGGTTCTTGGATTTGT+AGG - tig0028900:34586-34605 Msa1439870:intron 30.0%
! ATTTTCACCACTAGACTTTT+TGG - tig0028900:34798-34817 Msa1439870:intron 30.0%
TGCCTATTTACTGATATAAG+TGG - tig0028900:34977-34996 Msa1439870:intron 30.0%
CACCACTTATATCAGTAAAT+AGG + tig0028900:34982-35001 None:intergenic 30.0%
CTATTTACTGATATAAGTGG+TGG - tig0028900:34980-34999 Msa1439870:intron 30.0%
TTTCTTAGCAAACTTGACAA+TGG - tig0028900:35279-35298 Msa1439870:CDS 30.0%
ACAATGGATATAACAGCTAT+TGG - tig0028900:35295-35314 Msa1439870:CDS 30.0%
ATAACAGCTATTGGAACAAA+AGG - tig0028900:35304-35323 Msa1439870:CDS 30.0%
!! AATGAAGCATCTTTTCATGT+TGG - tig0028900:35367-35386 Msa1439870:CDS 30.0%
! TTGATGATCTTGTTACTTGT+TGG - tig0028900:35404-35423 Msa1439870:CDS 30.0%
! TGTGTTTTCCTACTAACAAT+TGG + tig0028900:35553-35572 None:intergenic 30.0%
CTGTTAAGTCTTCAATTCAT+AGG - tig0028900:35587-35606 Msa1439870:five_prime_UTR 30.0%
GTTAAGTCTTCAATTCATAG+GGG - tig0028900:35589-35608 Msa1439870:five_prime_UTR 30.0%
!! ATCACATTTTGAGAAGATAC+AGG + tig0028900:35670-35689 None:intergenic 30.0%
AGAAACTTTACGAGCTATGT+CGG + tig0028900:33631-33650 None:intergenic 35.0%
GAAGCAAATGCTTCTTATTC+TGG + tig0028900:33863-33882 None:intergenic 35.0%
TGCTGAGTTTGATGAAATTG+TGG - tig0028900:33908-33927 Msa1439870:CDS 35.0%
GCTGAGTTTGATGAAATTGT+GGG - tig0028900:33909-33928 Msa1439870:CDS 35.0%
TAATCCTAGGACTGCTAAAA+TGG - tig0028900:33983-34002 Msa1439870:CDS 35.0%
CCAAAAATAACAGTAGAGCT+AGG + tig0028900:34075-34094 None:intergenic 35.0%
!!! CCTAGCTCTACTGTTATTTT+TGG - tig0028900:34072-34091 Msa1439870:intron 35.0%
TAAGACTTTCAGACTTGAGA+AGG + tig0028900:34126-34145 None:intergenic 35.0%
TGTAGTCAAAGTCAAGATGT+AGG - tig0028900:34322-34341 Msa1439870:intron 35.0%
TCAAAGTCAAGATGTAGGAT+TGG - tig0028900:34327-34346 Msa1439870:intron 35.0%
TCATGTTTACGTTCCTTCAT+TGG + tig0028900:34357-34376 None:intergenic 35.0%
AATGAAGGAACGTAAACATG+AGG - tig0028900:34356-34375 Msa1439870:intron 35.0%
ACGTAACACAATTCGTAAGT+AGG - tig0028900:34380-34399 Msa1439870:intron 35.0%
ACACAATTCGTAAGTAGGAT+CGG - tig0028900:34385-34404 Msa1439870:intron 35.0%
TTGTAGTAAAGATCGAGTGT+TGG + tig0028900:34551-34570 None:intergenic 35.0%
ACACTCGATCTTTACTACAA+TGG - tig0028900:34551-34570 Msa1439870:intron 35.0%
AATCAAATTTGTCGCTCTGT+TGG - tig0028900:34681-34700 Msa1439870:intron 35.0%
ATCAAATTTGTCGCTCTGTT+GGG - tig0028900:34682-34701 Msa1439870:intron 35.0%
GACTGCAAAGTTTGATATGT+TGG - tig0028900:34753-34772 Msa1439870:intron 35.0%
ACTTTGTCCAAAAAGTCTAG+TGG + tig0028900:34808-34827 None:intergenic 35.0%
!! ATTGATAGTGTGGGAGATTA+AGG - tig0028900:34870-34889 Msa1439870:intron 35.0%
GATGGAGTTATATTGGATTG+TGG - tig0028900:35208-35227 Msa1439870:CDS 35.0%
ATTGTGGTGCATCACTATAT+TGG - tig0028900:35224-35243 Msa1439870:CDS 35.0%
TTGTGGTGCATCACTATATT+GGG - tig0028900:35225-35244 Msa1439870:CDS 35.0%
GATGCTTCATTCTCTTCATA+AGG + tig0028900:35358-35377 None:intergenic 35.0%
TAAAACTGATCTTCCTCAAG+AGG - tig0028900:35453-35472 Msa1439870:exon 35.0%
CAAAATGTGATCTGCTCAAT+TGG - tig0028900:35679-35698 Msa1439870:five_prime_UTR 35.0%
GAGTGTGGTATCTATGTACT+TGG + tig0028900:33522-33541 None:intergenic 40.0%
! GATCAGTGCTTCTGACATTT+TGG + tig0028900:33586-33605 None:intergenic 40.0%
!! TCAGAAGCACTGATCCAAAT+CGG - tig0028900:33591-33610 Msa1439870:CDS 40.0%
AACAAGCTTGCACCTTAAGT+GGG - tig0028900:33824-33843 Msa1439870:CDS 40.0%
!! GGATAATGGGGTTCAGTTTA+TGG - tig0028900:33941-33960 Msa1439870:CDS 40.0%
CTATGTGGGTTCATAATCCT+AGG - tig0028900:33970-33989 Msa1439870:CDS 40.0%
! AGTCAAGATGTAGGATTGGT+CGG - tig0028900:34331-34350 Msa1439870:intron 40.0%
AATTCGTAAGTAGGATCGGT+AGG - tig0028900:34389-34408 Msa1439870:intron 40.0%
CAGAGCGACAAATTTGATTG+AGG + tig0028900:34680-34699 None:intergenic 40.0%
CTGATATAAGTGGTGGCTTT+CGG - tig0028900:34987-35006 Msa1439870:intron 40.0%
TGATATAAGTGGTGGCTTTC+GGG - tig0028900:34988-35007 Msa1439870:intron 40.0%
ATCTGCAGCAAATGTAAAGC+AGG + tig0028900:35029-35048 None:intergenic 40.0%
!!! TGTTTTAGGGAGTTCGTAGT+TGG + tig0028900:35155-35174 None:intergenic 40.0%
! GTGAGGCTATCACTGTTTTA+GGG + tig0028900:35168-35187 None:intergenic 40.0%
GAACCAGTGCTTAACAAAGA+TGG - tig0028900:35190-35209 Msa1439870:CDS 40.0%
ACTCCATCTTTGTTAAGCAC+TGG + tig0028900:35196-35215 None:intergenic 40.0%
! GGTTACAGAATTTGCTCGTT+TGG - tig0028900:35483-35502 Msa1439870:five_prime_UTR 40.0%
! TACAGAATTTGCTCGTTTGG+TGG - tig0028900:35486-35505 Msa1439870:five_prime_UTR 40.0%
! ACAGAATTTGCTCGTTTGGT+GGG - tig0028900:35487-35506 Msa1439870:five_prime_UTR 40.0%
AGAAGTGGCCAATTGTTAGT+AGG - tig0028900:35542-35561 Msa1439870:five_prime_UTR 40.0%
! TTTTGAGCCTGTTCAGATTG+AGG - tig0028900:35612-35631 Msa1439870:five_prime_UTR 40.0%
TTTGAGAAGATACAGGCATG+TGG + tig0028900:35663-35682 None:intergenic 40.0%
ATCTGCTCAATTGGCATCTT+TGG - tig0028900:35688-35707 Msa1439870:five_prime_UTR 40.0%
ATGCATTTGTGAGCTGTTCA+TGG - tig0028900:35757-35776 Msa1439870:five_prime_UTR 40.0%
AAGATAGAAGTCCCCCTACA+AGG + tig0028900:33235-33254 None:intergenic 45.0%
CTGATCCAAATCGGAGTAGT+AGG - tig0028900:33600-33619 Msa1439870:CDS 45.0%
TATACACAGCGTCCACATCA+GGG + tig0028900:33794-33813 None:intergenic 45.0%
ATATACACAGCGTCCACATC+AGG + tig0028900:33795-33814 None:intergenic 45.0%
CACTTAAGGTGCAAGCTTGT+TGG + tig0028900:33825-33844 None:intergenic 45.0%
CAACAAGCTTGCACCTTAAG+TGG - tig0028900:33823-33842 Msa1439870:CDS 45.0%
TGAAATTGTGGGAGCTTGTG+AGG - tig0028900:33920-33939 Msa1439870:CDS 45.0%
TGGGAGCTTGTGAGGATAAT+GGG - tig0028900:33928-33947 Msa1439870:CDS 45.0%
!!! TGTGCCATTTTAGCAGTCCT+AGG + tig0028900:33990-34009 None:intergenic 45.0%
!! CCGATTTGAGTTGACCAAAG+CGG + tig0028900:34031-34050 None:intergenic 45.0%
CCGCTTTGGTCAACTCAAAT+CGG - tig0028900:34028-34047 Msa1439870:intron 45.0%
TAGGCAATGAACATGCAGTC+AGG + tig0028900:34963-34982 None:intergenic 45.0%
! CGTGAGGCTATCACTGTTTT+AGG + tig0028900:35169-35188 None:intergenic 45.0%
TGGTGCATCACTATATTGGG+AGG - tig0028900:35228-35247 Msa1439870:CDS 45.0%
GCATCACTATATTGGGAGGA+TGG - tig0028900:35232-35251 Msa1439870:CDS 45.0%
ACTCTAAGCCTGAGAAGAAG+TGG - tig0028900:35527-35546 Msa1439870:five_prime_UTR 45.0%
ACAATTGGCCACTTCTTCTC+AGG + tig0028900:35538-35557 None:intergenic 45.0%
CTTCACTCCTCAATCTGAAC+AGG + tig0028900:35622-35641 None:intergenic 45.0%
TCAGATTGAGGAGTGAAGCA+AGG - tig0028900:35624-35643 Msa1439870:five_prime_UTR 45.0%
TAGACTGAGCTGAGTGAGTG+TGG + tig0028900:33537-33556 None:intergenic 50.0%
CACAGCCTACTACTCCGATT+TGG + tig0028900:33608-33627 None:intergenic 50.0%
CGTATACCTTCGCTGTCAGA+GGG + tig0028900:33746-33765 None:intergenic 50.0%
! CCTCTGACAGCGAAGGTATA+CGG - tig0028900:33744-33763 Msa1439870:CDS 50.0%
TCTACTGGAAGACCCTGATG+TGG - tig0028900:33779-33798 Msa1439870:CDS 50.0%
!! AGGTGCAAGCTTGTTGGTAG+TGG + tig0028900:33819-33838 None:intergenic 50.0%
CTTATTCTGGGCCGCAAGAA+CGG + tig0028900:33850-33869 None:intergenic 50.0%
TCAGCAATATCCAGCGCAAC+GGG + tig0028900:33894-33913 None:intergenic 50.0%
CTCAGCAATATCCAGCGCAA+CGG + tig0028900:33895-33914 None:intergenic 50.0%
GTGGGAGCTTGTGAGGATAA+TGG - tig0028900:33927-33946 Msa1439870:CDS 50.0%
GGGAGCTTGTGAGGATAATG+GGG - tig0028900:33929-33948 Msa1439870:CDS 50.0%
TTCAATGATCCGCACCGCTT+TGG - tig0028900:34014-34033 Msa1439870:CDS 50.0%
!! TTGAGTTGACCAAAGCGGTG+CGG + tig0028900:34026-34045 None:intergenic 50.0%
!! TAGGATTGGTCGGCCAATGA+AGG - tig0028900:34341-34360 Msa1439870:intron 50.0%
GTAACATCGAAGCACGGTTG+AGG - tig0028900:34504-34523 Msa1439870:intron 50.0%
!! AAGCACGGTTGAGGTGTTGT+GGG - tig0028900:34513-34532 Msa1439870:intron 50.0%
AAGCCAGATCTTGACGCACA+TGG - tig0028900:35079-35098 Msa1439870:CDS 50.0%
GAACCATGTGCGTCAAGATC+TGG + tig0028900:35085-35104 None:intergenic 50.0%
CTTGACGCACATGGTTCTCT+AGG - tig0028900:35088-35107 Msa1439870:CDS 50.0%
CATGGTTCTCTAGGTGATGC+AGG - tig0028900:35097-35116 Msa1439870:CDS 50.0%
GTTCTCTAGGTGATGCAGGT+TGG - tig0028900:35101-35120 Msa1439870:CDS 50.0%
GTTCGTAGTTGGCAGCTAAG+AGG + tig0028900:35144-35163 None:intergenic 50.0%
!! GTTAAGCACTGGTTCACGTG+AGG + tig0028900:35185-35204 None:intergenic 50.0%
TCTTCCTCAAGAGGCTCTCT+TGG - tig0028900:35462-35481 Msa1439870:exon 50.0%
GTAACCAAGAGAGCCTCTTG+AGG + tig0028900:35469-35488 None:intergenic 50.0%
! GGCGTTTGGGGATAGGTTGA+TGG + tig0028900:33658-33677 None:intergenic 55.0%
! AGATGGTGGCGTTTGGGGAT+AGG + tig0028900:33665-33684 None:intergenic 55.0%
TGTCAGAGGGTAGCCATTGG+CGG + tig0028900:33733-33752 None:intergenic 55.0%
CCGTATACCTTCGCTGTCAG+AGG + tig0028900:33747-33766 None:intergenic 55.0%
CGGCAACTACGAAGCTCTAC+TGG - tig0028900:33764-33783 Msa1439870:CDS 55.0%
CCTTAAGTGGGCCGTTCTTG+CGG - tig0028900:33836-33855 Msa1439870:CDS 55.0%
CCGTGCTTCGATGTTACGCA+GGG + tig0028900:34501-34520 None:intergenic 55.0%
CCCTGCGTAACATCGAAGCA+CGG - tig0028900:34498-34517 Msa1439870:intron 55.0%
ACCGTGCTTCGATGTTACGC+AGG + tig0028900:34502-34521 None:intergenic 55.0%
!! GAAGCACGGTTGAGGTGTTG+TGG - tig0028900:34512-34531 Msa1439870:intron 55.0%
! GGCTTTGTCGTAGTTGCGGC+TGG + tig0028900:33697-33716 None:intergenic 60.0%
! CGGCGGCTTTGTCGTAGTTG+CGG + tig0028900:33701-33720 None:intergenic 60.0%
CGCTGTCAGAGGGTAGCCAT+TGG + tig0028900:33736-33755 None:intergenic 60.0%
TGGCTACCCTCTGACAGCGA+AGG - tig0028900:33737-33756 Msa1439870:CDS 60.0%
CCGCAAGAACGGCCCACTTA+AGG + tig0028900:33839-33858 None:intergenic 60.0%
TCTTGAGAAGCCCGTTGCGC+TGG - tig0028900:33881-33900 Msa1439870:CDS 60.0%
TGGTGGCTTTCGGGCTGTAC+AGG - tig0028900:34997-35016 Msa1439870:intron 60.0%
! GGCGCAGATGGTGGCGTTTG+GGG + tig0028900:33670-33689 None:intergenic 65.0%
! CGGCGCAGATGGTGGCGTTT+GGG + tig0028900:33671-33690 None:intergenic 65.0%
! ACGGCGCAGATGGTGGCGTT+TGG + tig0028900:33672-33691 None:intergenic 65.0%
GTCGTAGTTGCGGCTGGCGA+CGG + tig0028900:33691-33710 None:intergenic 65.0%
ATTGGCGGTGGCGAAAGCGG+CGG + tig0028900:33718-33737 None:intergenic 65.0%
GCCATTGGCGGTGGCGAAAG+CGG + tig0028900:33721-33740 None:intergenic 65.0%
CAGAGGGTAGCCATTGGCGG+TGG + tig0028900:33730-33749 None:intergenic 65.0%
! GCCGCTTTCGCCACCGCCAA+TGG - tig0028900:33717-33736 Msa1439870:CDS 70.0%
GCTGGCGACGGCGCAGATGG+TGG + tig0028900:33679-33698 None:intergenic 75.0%
! GCGGCTGGCGACGGCGCAGA+TGG + tig0028900:33682-33701 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
tig0028900 gene 33223 35833 33223 ID=Msa1439870;Name=Msa1439870
tig0028900 mRNA 33223 35833 33223 ID=Msa1439870-mRNA-1;Parent=Msa1439870;Name=Msa1439870-mRNA-1;_AED=0.02;_eAED=0.02;_QI=365|1|1|1|1|1|2|194|360
tig0028900 exon 33223 34037 33223 ID=Msa1439870-mRNA-1:exon:11969;Parent=Msa1439870-mRNA-1
tig0028900 exon 35007 35833 35007 ID=Msa1439870-mRNA-1:exon:11968;Parent=Msa1439870-mRNA-1
tig0028900 five_prime_UTR 35469 35833 35469 ID=Msa1439870-mRNA-1:five_prime_utr;Parent=Msa1439870-mRNA-1
tig0028900 CDS 35007 35468 35007 ID=Msa1439870-mRNA-1:cds;Parent=Msa1439870-mRNA-1
tig0028900 CDS 33417 34037 33417 ID=Msa1439870-mRNA-1:cds;Parent=Msa1439870-mRNA-1
tig0028900 three_prime_UTR 33223 33416 33223 ID=Msa1439870-mRNA-1:three_prime_utr;Parent=Msa1439870-mRNA-1
Gene Sequence

>Msa1439870

ATGTCAGAAGCACTGATCCAAATCGGAGTAGTAGGCTGTGCCGACATAGCTCGTAAAGTTTCTCGCGCCATCAACCTATCCCCAAACGCCACCATCTGCGCCGTCGCCAGCCGCAACTACGACAAAGCCGCCGCTTTCGCCACCGCCAATGGCTACCCTCTGACAGCGAAGGTATACGGCAACTACGAAGCTCTACTGGAAGACCCTGATGTGGACGCTGTGTATATGCCACTACCAACAAGCTTGCACCTTAAGTGGGCCGTTCTTGCGGCCCAGAATAAGAAGCATTTGCTTCTTGAGAAGCCCGTTGCGCTGGATATTGCTGAGTTTGATGAAATTGTGGGAGCTTGTGAGGATAATGGGGTTCAGTTTATGGATAATACTATGTGGGTTCATAATCCTAGGACTGCTAAAATGGCACAGTTCTTCAATGATCCGCACCGCTTTGGTCAACTCAAATCGGTTCGCACCTGCTTTACATTTGCTGCAGATTCTGAATATCTAAAGAAAGACATTCGTGTGAAGCCAGATCTTGACGCACATGGTTCTCTAGGTGATGCAGGTTGGTATTGCATTCGTGCAATCCTCTTAGCTGCCAACTACGAACTCCCTAAAACAGTGATAGCCTCACGTGAACCAGTGCTTAACAAAGATGGAGTTATATTGGATTGTGGTGCATCACTATATTGGGAGGATGGTAAAGTAGCTACATTTCATTGCTCTTTCTTAGCAAACTTGACAATGGATATAACAGCTATTGGAACAAAAGGAACTCTTCATGTTCATGATTTTATTATTCCTTATGAAGAGAATGAAGCATCTTTTCATGTTGGTACTGAAACTAGCTTTGATGATCTTGTTACTTGTTGGTCTAAACAGCCAATTAAGTGTACTGTTAAAACTGATCTTCCTCAAGAGGCTCTCTTGGTTACAGAATTTGCTCGTTTGGTGGGTGAAATTAAATTTAGAAACTCTAAGCCTGAGAAGAAGTGGCCAATTGTTAGTAGGAAAACACAACTTGTGTTGAATGCTGTTAAGTCTTCAATTCATAGGGGTTTTGAGCCTGTTCAGATTGAGGAGTGA
Protein sequence

>Msa1439870

MSEALIQIGVVGCADIARKVSRAINLSPNATICAVASRNYDKAAAFATANGYPLTAKVYGNYEALLEDPDVDAVYMPLPTSLHLKWAVLAAQNKKHLLLEKPVALDIAEFDEIVGACEDNGVQFMDNTMWVHNPRTAKMAQFFNDPHRFGQLKSVRTCFTFAADSEYLKKDIRVKPDLDAHGSLGDAGWYCIRAILLAANYELPKTVIASREPVLNKDGVILDCGASLYWEDGKVATFHCSFLANLTMDITAIGTKGTLHVHDFIIPYEENEASFHVGTETSFDDLVTCWSKQPIKCTVKTDLPQEALLVTEFARLVGEIKFRNSKPEKKWPIVSRKTQLVLNAVKSSIHRGFEPVQIEE*