AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0189350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATTCCATTCTAAGTATC+TGG 0.247640 2_1:-4763624 None:intergenic
TATGTAATTTAAAATAAATT+AGG 0.313236 2_1:+4763869 Msa0189350:three_prime_UTR
ACACTTAATTCTGGCGAGTT+AGG 0.330208 2_1:+4763662 Msa0189350:CDS
TAGTACTTGTAAGTGTTGTT+AGG 0.376359 2_1:+4763788 Msa0189350:three_prime_UTR
TGGATGGATACACTTAATTC+TGG 0.408249 2_1:+4763653 Msa0189350:CDS
ATTAATTTAATTGATACAAA+TGG 0.438485 2_1:-4763977 None:intergenic
GTACTTGTAAGCACTGATAT+AGG 0.448750 2_1:+4763747 Msa0189350:three_prime_UTR
GTAATTTAACAATGTCTTGA+AGG 0.457400 2_1:-4763955 None:intergenic
TTGTTACCAGATACTTAGAA+TGG 0.491496 2_1:+4763618 Msa0189350:CDS
ATGGAATGTGAGAAGGTGGA+TGG 0.536531 2_1:+4763637 Msa0189350:CDS
TAATTTAACAATGTCTTGAA+GGG 0.548774 2_1:-4763954 None:intergenic
TAGAATGGAATGTGAGAAGG+TGG 0.592204 2_1:+4763633 Msa0189350:CDS
ACTTAGAATGGAATGTGAGA+AGG 0.609504 2_1:+4763630 Msa0189350:CDS
TAACACCGGAAATCTTCAGA+TGG 0.624072 2_1:-4763010 None:intergenic
AATCAATTGAAGTTTAACAC+CGG 0.629175 2_1:-4763024 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAAAAATTAAAAAA+AGG + chr2_1:4761361-4761380 Msa0189350:intron 0.0%
!!! AATTTATTTATTTTCAAGTT+GGG + chr2_1:4761612-4761631 Msa0189350:intron 10.0%
!! ATACAAAAAAAACAAAAAAA+AGG - chr2_1:4761912-4761931 None:intergenic 10.0%
!! ATTAATTTAATTGATACAAA+TGG - chr2_1:4763980-4763999 None:intergenic 10.0%
!!! CAATTTATTTATTTTCAAGT+TGG + chr2_1:4761611-4761630 Msa0189350:intron 15.0%
!! AAAAAAAACAAAAAAAAGGA+TGG - chr2_1:4761908-4761927 None:intergenic 15.0%
!!! CTTTAGTCTTTTTTTTTTAA+AGG - chr2_1:4762202-4762221 None:intergenic 15.0%
!!! TAAAGAATCTGAAATATATA+TGG + chr2_1:4763571-4763590 Msa0189350:intron 15.0%
!! ACTAAAGAAAAACTGAATAT+TGG + chr2_1:4762215-4762234 Msa0189350:intron 20.0%
!! CTAAAGAAAAACTGAATATT+GGG + chr2_1:4762216-4762235 Msa0189350:intron 20.0%
!!! CTTGATATTTATTTTCTGTT+GGG - chr2_1:4762551-4762570 None:intergenic 20.0%
!!! TCTTGATATTTATTTTCTGT+TGG - chr2_1:4762552-4762571 None:intergenic 20.0%
!!! TTTATTTATTTCTTCCACAA+AGG + chr2_1:4762581-4762600 Msa0189350:intron 20.0%
!!! TTATTTATTTCTTCCACAAA+GGG + chr2_1:4762582-4762601 Msa0189350:intron 20.0%
!!! AATTTATTTTGTCAAACTCT+AGG - chr2_1:4762850-4762869 None:intergenic 20.0%
!!! TCTTTAGAATATAAACTCAA+GGG + chr2_1:4762877-4762896 Msa0189350:intron 20.0%
!! ACCATTCATTTGTAATTAAT+TGG + chr2_1:4762955-4762974 Msa0189350:intron 20.0%
!! ACCAATTAATTACAAATGAA+TGG - chr2_1:4762959-4762978 None:intergenic 20.0%
!! AACTAATATCTCTAATTGTT+TGG - chr2_1:4763334-4763353 None:intergenic 20.0%
!! TAAAGCAAAAAACACAAATT+GGG - chr2_1:4763702-4763721 None:intergenic 20.0%
!! ATAAAGCAAAAAACACAAAT+TGG - chr2_1:4763703-4763722 None:intergenic 20.0%
!! TAATTTAACAATGTCTTGAA+GGG - chr2_1:4763957-4763976 None:intergenic 20.0%
! GAAAAAGAGATATATGTCAA+AGG - chr2_1:4761309-4761328 None:intergenic 25.0%
!!! TGACATATATCTCTTTTTCA+TGG + chr2_1:4761310-4761329 Msa0189350:intron 25.0%
!!! GACATATATCTCTTTTTCAT+GGG + chr2_1:4761311-4761330 Msa0189350:intron 25.0%
!!! TCTTTCATTATTTTAACCAG+TGG + chr2_1:4761937-4761956 Msa0189350:intron 25.0%
! TAATTATATGTGTCTTTAGC+AGG + chr2_1:4761961-4761980 Msa0189350:intron 25.0%
! AAAAACTAAGACATCCAATT+TGG - chr2_1:4762163-4762182 None:intergenic 25.0%
!!! ATATTTATTTTCTGTTGGGA+AGG - chr2_1:4762547-4762566 None:intergenic 25.0%
!! TCTAAAATAAAATCCCTTTG+TGG - chr2_1:4762598-4762617 None:intergenic 25.0%
!! TTTTTGCTTATATTAGTGAC+CGG + chr2_1:4762755-4762774 Msa0189350:intron 25.0%
!!! TCAAGACATTTTTTAGGTTT+GGG - chr2_1:4762792-4762811 None:intergenic 25.0%
!!! CTCTTTAGAATATAAACTCA+AGG + chr2_1:4762876-4762895 Msa0189350:intron 25.0%
! AATCAATTGAAGTTTAACAC+CGG - chr2_1:4763027-4763046 None:intergenic 25.0%
!!! GAAGGTTTTGTTAATAGAAA+CGG - chr2_1:4763285-4763304 None:intergenic 25.0%
! TCTATTAACAAAACCTTCTA+TGG + chr2_1:4763287-4763306 Msa0189350:intron 25.0%
!! AATTGTTTGGAATTAAGTGT+TGG - chr2_1:4763321-4763340 None:intergenic 25.0%
! TGGAGAAAGATGATTATTAA+TGG + chr2_1:4763446-4763465 Msa0189350:intron 25.0%
! GGAGAAAGATGATTATTAAT+GGG + chr2_1:4763447-4763466 Msa0189350:intron 25.0%
! GAGAAAGATGATTATTAATG+GGG + chr2_1:4763448-4763467 Msa0189350:intron 25.0%
!!! TTGTGTTTTTTGCTTTATGT+TGG + chr2_1:4763705-4763724 Msa0189350:CDS 25.0%
! GTAATTTAACAATGTCTTGA+AGG - chr2_1:4763958-4763977 None:intergenic 25.0%
! TGTTCTATCTATGCACTTTT+TGG - chr2_1:4761447-4761466 None:intergenic 30.0%
! TCACATATCCTCATGATTTT+AGG + chr2_1:4761521-4761540 Msa0189350:intron 30.0%
ACTTTGTACCTAAAATCATG+AGG - chr2_1:4761532-4761551 None:intergenic 30.0%
TGTATGTGTAAGTCTAACTA+AGG - chr2_1:4761560-4761579 None:intergenic 30.0%
TTGGTATTTCGTAGTATTTG+AGG - chr2_1:4761678-4761697 None:intergenic 30.0%
!!! GTATTTTTTGGTAGAGTTGA+GGG - chr2_1:4761880-4761899 None:intergenic 30.0%
!!! AGTATTTTTTGGTAGAGTTG+AGG - chr2_1:4761881-4761900 None:intergenic 30.0%
AGACACATATAATTATCCAC+TGG - chr2_1:4761956-4761975 None:intergenic 30.0%
!!! CAAGAGTTTTTTTAGCCTTA+AGG - chr2_1:4761992-4762011 None:intergenic 30.0%
!! TTGTCTGTTGGTATTGATTA+AGG + chr2_1:4762059-4762078 Msa0189350:intron 30.0%
TTCTCGTGAATTAACCAAAT+TGG + chr2_1:4762146-4762165 Msa0189350:intron 30.0%
CATCTCAAAATGGTTTAATG+TGG - chr2_1:4762386-4762405 None:intergenic 30.0%
ATGGTAAGTATCAAAATGCA+AGG - chr2_1:4762432-4762451 None:intergenic 30.0%
! TTTGATACTTACCATTTTGC+TGG + chr2_1:4762437-4762456 Msa0189350:intron 30.0%
TGCTTATATTAGTGACTAGA+GGG - chr2_1:4762642-4762661 None:intergenic 30.0%
TTGCTTATATTAGTGACTAG+AGG - chr2_1:4762643-4762662 None:intergenic 30.0%
!!! CTCAAGACATTTTTTAGGTT+TGG - chr2_1:4762793-4762812 None:intergenic 30.0%
GTTTGAATGAATTTGAGTGT+TGG + chr2_1:4762902-4762921 Msa0189350:intron 30.0%
AATTGGTTGTTTGAAAATCC+AGG + chr2_1:4762972-4762991 Msa0189350:intron 30.0%
! TTTTACCATCTGAAGATTTC+CGG + chr2_1:4763005-4763024 Msa0189350:CDS 30.0%
!! TTGTTAGATTCGAGTTTTGT+TGG + chr2_1:4763052-4763071 Msa0189350:intron 30.0%
GACTACAAACATAACTGTAT+TGG - chr2_1:4763183-4763202 None:intergenic 30.0%
TTGTTACCAGATACTTAGAA+TGG + chr2_1:4763618-4763637 Msa0189350:CDS 30.0%
AAAAACACAAATTGGGCAAT+AGG - chr2_1:4763695-4763714 None:intergenic 30.0%
! TAGTACTTGTAAGTGTTGTT+AGG + chr2_1:4763788-4763807 Msa0189350:three_prime_UTR 30.0%
TATGGGTGGAATTGGAAAAA+AGG + chr2_1:4761242-4761261 Msa0189350:intron 35.0%
AGAACAACTCTCATGTCATA+AGG + chr2_1:4761461-4761480 Msa0189350:intron 35.0%
TGTGTAAGTCTAACTAAGGT+TGG - chr2_1:4761556-4761575 None:intergenic 35.0%
CTACGAAATACCAACATAAC+CGG + chr2_1:4761684-4761703 Msa0189350:intron 35.0%
!!! AGGATGGAAGCAGTATTTTT+TGG - chr2_1:4761892-4761911 None:intergenic 35.0%
CTTTAGCAGGAAAAACCTTA+AGG + chr2_1:4761974-4761993 Msa0189350:intron 35.0%
CTCTTGCAAAGAGACATTAA+CGG + chr2_1:4762006-4762025 Msa0189350:intron 35.0%
AATCCAGAATCTCCTATCTA+AGG + chr2_1:4762035-4762054 Msa0189350:intron 35.0%
AAACCTTAGATAGGAGATTC+TGG - chr2_1:4762041-4762060 None:intergenic 35.0%
TATTGTTGTGCATGAGATAG+TGG + chr2_1:4762303-4762322 Msa0189350:intron 35.0%
! GCTAGATGGAAATGGAATTT+TGG - chr2_1:4762354-4762373 None:intergenic 35.0%
TATGTCATTAGCCAGCAAAA+TGG - chr2_1:4762451-4762470 None:intergenic 35.0%
!! GGAAGGAAAATAGTTTGCTT+TGG - chr2_1:4762530-4762549 None:intergenic 35.0%
!!! GGGTTCTCAAGACATTTTTT+AGG - chr2_1:4762798-4762817 None:intergenic 35.0%
! AATTTGCTTCTTCGACTCTT+GGG - chr2_1:4762818-4762837 None:intergenic 35.0%
! AAATTTGCTTCTTCGACTCT+TGG - chr2_1:4762819-4762838 None:intergenic 35.0%
! GTCGAAGAAGCAAATTTTCA+AGG + chr2_1:4762822-4762841 Msa0189350:intron 35.0%
TATACTTACCTGTCTAGTCA+AGG - chr2_1:4763106-4763125 None:intergenic 35.0%
!!! TTGAAGTTGGTGTTTTGGAT+TGG + chr2_1:4763404-4763423 Msa0189350:intron 35.0%
TCCATGGCATCTTAATTAGT+TGG + chr2_1:4763503-4763522 Msa0189350:intron 35.0%
CCATGGCATCTTAATTAGTT+GGG + chr2_1:4763504-4763523 Msa0189350:intron 35.0%
TATGGTTTAAATGCGTTTGC+AGG + chr2_1:4763589-4763608 Msa0189350:intron 35.0%
CACATTCCATTCTAAGTATC+TGG - chr2_1:4763627-4763646 None:intergenic 35.0%
ACTTAGAATGGAATGTGAGA+AGG + chr2_1:4763630-4763649 Msa0189350:CDS 35.0%
TGGATGGATACACTTAATTC+TGG + chr2_1:4763653-4763672 Msa0189350:CDS 35.0%
!! GTACTTGTAAGCACTGATAT+AGG + chr2_1:4763747-4763766 Msa0189350:three_prime_UTR 35.0%
GTTGCTGCAATATGGTATGA+GGG - chr2_1:4761717-4761736 None:intergenic 40.0%
AAGATCTGGTTGCTGCAATA+TGG - chr2_1:4761725-4761744 None:intergenic 40.0%
TGGTAGAGTTGAGGGAAAAA+AGG - chr2_1:4761872-4761891 None:intergenic 40.0%
CCAACAGACAAACCTTAGAT+AGG - chr2_1:4762050-4762069 None:intergenic 40.0%
! CCTATCTAAGGTTTGTCTGT+TGG + chr2_1:4762047-4762066 Msa0189350:intron 40.0%
CCAATGTAGCTAGATGGAAA+TGG - chr2_1:4762362-4762381 None:intergenic 40.0%
CCATTTCCATCTAGCTACAT+TGG + chr2_1:4762359-4762378 Msa0189350:intron 40.0%
ACTGCTGCTACATCTCAAAA+TGG - chr2_1:4762396-4762415 None:intergenic 40.0%
TTGCTTATATTAGTGACCGG+AGG + chr2_1:4762758-4762777 Msa0189350:intron 40.0%
TGCTTATATTAGTGACCGGA+GGG + chr2_1:4762759-4762778 Msa0189350:intron 40.0%
GGTTGTTTGAAAATCCAGGA+AGG + chr2_1:4762976-4762995 Msa0189350:intron 40.0%
TAACACCGGAAATCTTCAGA+TGG - chr2_1:4763013-4763032 None:intergenic 40.0%
TCACATGCCAAATCCAATAC+AGG - chr2_1:4763223-4763242 None:intergenic 40.0%
AGACAAAAGGCTTATGAGAG+AGG - chr2_1:4763366-4763385 None:intergenic 40.0%
AACACCAACTTCAAACTGCT+GGG - chr2_1:4763398-4763417 None:intergenic 40.0%
AAACACCAACTTCAAACTGC+TGG - chr2_1:4763399-4763418 None:intergenic 40.0%
!!! GCAGTTTGAAGTTGGTGTTT+TGG + chr2_1:4763399-4763418 Msa0189350:intron 40.0%
!!! GTTGGTGTTTTGGATTGGTT+TGG + chr2_1:4763409-4763428 Msa0189350:intron 40.0%
TGGGGATATATCACAAACTG+TGG + chr2_1:4763466-4763485 Msa0189350:intron 40.0%
CCCAACTAATTAAGATGCCA+TGG - chr2_1:4763507-4763526 None:intergenic 40.0%
TAGAATGGAATGTGAGAAGG+TGG + chr2_1:4763633-4763652 Msa0189350:CDS 40.0%
ACACTTAATTCTGGCGAGTT+AGG + chr2_1:4763662-4763681 Msa0189350:CDS 40.0%
ACACATACACTTGCTCACTC+AGG + chr2_1:4761571-4761590 Msa0189350:intron 45.0%
CACATACACTTGCTCACTCA+GGG + chr2_1:4761572-4761591 Msa0189350:intron 45.0%
ACATACACTTGCTCACTCAG+GGG + chr2_1:4761573-4761592 Msa0189350:intron 45.0%
ATGGTATGAGGGAGGTAAAC+CGG - chr2_1:4761706-4761725 None:intergenic 45.0%
GGTTGCTGCAATATGGTATG+AGG - chr2_1:4761718-4761737 None:intergenic 45.0%
TTCATCCACTTCTCCACCAA+CGG + chr2_1:4761763-4761782 Msa0189350:intron 45.0%
TGGTTGCTACAATGGTGTGA+GGG - chr2_1:4761796-4761815 None:intergenic 45.0%
GACAGATCTGGTTGCTACAA+TGG - chr2_1:4761804-4761823 None:intergenic 45.0%
TTCATCCACTTCTCCACCAA+CGG + chr2_1:4761763-4761782 Msa0189350:intron 45.0%
AAAAAGGATCCAACCGTTGG+TGG - chr2_1:4761856-4761875 None:intergenic 45.0%
GGAAAAAAGGATCCAACCGT+TGG - chr2_1:4761859-4761878 None:intergenic 45.0%
TGTGGACCAATGTAGCTAGA+TGG - chr2_1:4762368-4762387 None:intergenic 45.0%
TGGTAAAAACTGAGCCTTCC+TGG - chr2_1:4762993-4763012 None:intergenic 45.0%
ACTTACCTGTCTAGTCAAGG+AGG - chr2_1:4763103-4763122 None:intergenic 45.0%
GCCAAATCCAATACAGGTTG+CGG - chr2_1:4763217-4763236 None:intergenic 45.0%
GTTGGCGACAATACCATAGA+AGG - chr2_1:4763303-4763322 None:intergenic 45.0%
TGGGAGTCGACATAGACAAA+AGG - chr2_1:4763379-4763398 None:intergenic 45.0%
ATCACAAACTGTGGCACAGA+AGG + chr2_1:4763475-4763494 Msa0189350:intron 45.0%
ATGGAATGTGAGAAGGTGGA+TGG + chr2_1:4763637-4763656 Msa0189350:CDS 45.0%
!!! TTATTTTAAATTACATAATA+CGG - chr2_1:4763866-4763885 None:intergenic 5.0%
!!! TATGTAATTTAAAATAAATT+AGG + chr2_1:4763869-4763888 Msa0189350:three_prime_UTR 5.0%
CATACACTTGCTCACTCAGG+GGG + chr2_1:4761574-4761593 Msa0189350:intron 50.0%
GGGAGGTAAACCGGTTATGT+TGG - chr2_1:4761697-4761716 None:intergenic 50.0%
GCTGCAATATGGTATGAGGG+AGG - chr2_1:4761714-4761733 None:intergenic 50.0%
GCAGCAACCAGATCTTTCAC+TGG + chr2_1:4761729-4761748 Msa0189350:intron 50.0%
GCAGTCTCCAGTGAAAGATC+TGG - chr2_1:4761739-4761758 None:intergenic 50.0%
GGTGGAGAAGTGGATGAACA+CGG - chr2_1:4761761-4761780 None:intergenic 50.0%
! GTAAACCGTTGGTGGAGAAG+TGG - chr2_1:4761771-4761790 None:intergenic 50.0%
TTGCTACAATGGTGTGAGGG+GGG - chr2_1:4761793-4761812 None:intergenic 50.0%
GTTGCTACAATGGTGTGAGG+GGG - chr2_1:4761794-4761813 None:intergenic 50.0%
GGTTGCTACAATGGTGTGAG+GGG - chr2_1:4761795-4761814 None:intergenic 50.0%
CTGGTTGCTACAATGGTGTG+AGG - chr2_1:4761797-4761816 None:intergenic 50.0%
GTAGCAACCAGATCTGTCAC+TGG + chr2_1:4761806-4761825 Msa0189350:intron 50.0%
GCAGTTTCCAGTGACAGATC+TGG - chr2_1:4761816-4761835 None:intergenic 50.0%
GGTGGAGAAGTGGATGAACA+CGG - chr2_1:4761761-4761780 None:intergenic 50.0%
CTCAACCTCCTTGACTAGAC+AGG + chr2_1:4763095-4763114 Msa0189350:intron 50.0%
GAGTTTGCCGCAACCTGTAT+TGG + chr2_1:4763207-4763226 Msa0189350:intron 50.0%
GCCGCAACCTGTATTGGATT+TGG + chr2_1:4763213-4763232 Msa0189350:intron 50.0%
! GACTCCCAGCAGTTTGAAGT+TGG + chr2_1:4763391-4763410 Msa0189350:intron 50.0%
!! TGGATTGGTTTGGCAGACTG+TGG + chr2_1:4763419-4763438 Msa0189350:intron 50.0%
!! GGATTGGTTTGGCAGACTGT+GGG + chr2_1:4763420-4763439 Msa0189350:intron 50.0%
TCCACTTCTCCACCAACGGT+TGG + chr2_1:4761767-4761786 Msa0189350:intron 55.0%
! TCCAACCGTTGGTGGAGAAG+TGG - chr2_1:4761848-4761867 None:intergenic 55.0%
! GGTTTGGGATGTACACCCTC+CGG - chr2_1:4762777-4762796 None:intergenic 55.0%
GTTTGGCAGACTGTGGGAAG+TGG + chr2_1:4763426-4763445 Msa0189350:intron 55.0%
GGCACAGAAGGCAATCTCCA+TGG + chr2_1:4763487-4763506 Msa0189350:intron 55.0%
TGAGGGGGGTAAACCGTTGG+TGG - chr2_1:4761779-4761798 None:intergenic 60.0%
GTGTGAGGGGGGTAAACCGT+TGG - chr2_1:4761782-4761801 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 4761237 4764008 4761237 ID=Msa0189350;Name=Msa0189350
chr2_1 mRNA 4761237 4764008 4761237 ID=Msa0189350-mRNA-1;Parent=Msa0189350;Name=Msa0189350-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0|0.33|1|0|0.33|3|275|60
chr2_1 exon 4761237 4761240 4761237 ID=Msa0189350-mRNA-1:exon:2106;Parent=Msa0189350-mRNA-1
chr2_1 exon 4762994 4763049 4762994 ID=Msa0189350-mRNA-1:exon:2107;Parent=Msa0189350-mRNA-1
chr2_1 exon 4763611 4764008 4763611 ID=Msa0189350-mRNA-1:exon:2108;Parent=Msa0189350-mRNA-1
chr2_1 CDS 4761237 4761240 4761237 ID=Msa0189350-mRNA-1:cds;Parent=Msa0189350-mRNA-1
chr2_1 CDS 4762994 4763049 4762994 ID=Msa0189350-mRNA-1:cds;Parent=Msa0189350-mRNA-1
chr2_1 CDS 4763611 4763733 4763611 ID=Msa0189350-mRNA-1:cds;Parent=Msa0189350-mRNA-1
chr2_1 three_prime_UTR 4763734 4764008 4763734 ID=Msa0189350-mRNA-1:three_prime_utr;Parent=Msa0189350-mRNA-1
Gene Sequence

>Msa0189350

ATGGGAAGGCTCAGTTTTTACCATCTGAAGATTTCCGGTGTTAAACTTCAATTGATTCGAGAGAGAGTTGTTACCAGATACTTAGAATGGAATGTGAGAAGGTGGATGGATACACTTAATTCTGGCGAGTTAGGAGTTGAACCTATTGCCCAATTTGTGTTTTTTGCTTTATGTTGGAGTTGA

Protein sequence

>Msa0189350

MGRLSFYHLKISGVKLQLIRERVVTRYLEWNVRRWMDTLNSGELGVEPIAQFVFFALCWS*