AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0191930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191930 MtrunA17_Chr2g0281851 97.568 329 7 1 1 329 27 354 0.0 660
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191930 AT4G04930.1 81.682 333 56 2 1 329 1 332 0.0 567

Find 102 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCTCAATGGTTTATTTC+TGG 0.042011 2_1:-7666148 Msa0191930:CDS
AACCGAAACCTCCAGGTATT+TGG 0.110935 2_1:-7666205 Msa0191930:CDS
TAAACAAATGGCTTGCTATC+TGG 0.171894 2_1:+7665670 None:intergenic
ACATGATCATGGTCAGTTTA+AGG 0.208576 2_1:-7665462 Msa0191930:three_prime_UTR
GGTTATTTACATGTATATTA+TGG 0.220265 2_1:-7665853 Msa0191930:CDS
TTGTTTAATTTGAGGGTATT+TGG 0.221781 2_1:+7667809 None:intergenic
TTTCTCTTCATTCTGCTATA+AGG 0.250507 2_1:+7665816 None:intergenic
CCTCAACCACAATCTCTTCT+TGG 0.268270 2_1:-7666495 Msa0191930:CDS
ACAATGGTGATGAATGTTAT+TGG 0.292521 2_1:-7665637 Msa0191930:three_prime_UTR
TGTCTCAATGGTTTATTTCT+GGG 0.295906 2_1:-7666147 Msa0191930:CDS
TGATGGCTGGTGATTTCTTT+TGG 0.300981 2_1:-7667878 Msa0191930:CDS
ACTTTCTTTGTTGAAATGTT+TGG 0.306805 2_1:+7665586 None:intergenic
AGGCGTCGATGGCGTTGATA+TGG 0.307309 2_1:-7666333 Msa0191930:CDS
ACCTTGTGAAGTTTGTTTCC+AGG 0.308162 2_1:+7665933 None:intergenic
TCCCAAATACCTGGAGGTTT+CGG 0.315484 2_1:+7666203 None:intergenic
TAAGAATATGTCTCTTGTTC+AGG 0.318858 2_1:+7666026 None:intergenic
ACCGAAACCTCCAGGTATTT+GGG 0.325860 2_1:-7666204 Msa0191930:CDS
CCTCAAATTAAACAACTCTT+TGG 0.348261 2_1:-7667801 Msa0191930:CDS
AGTTTGTTTCCAGGAATTCT+TGG 0.349785 2_1:+7665942 None:intergenic
AAACAAATGGCTTGCTATCT+GGG 0.353317 2_1:+7665671 None:intergenic
GGTAAAGGCGGAGGAGGAGG+AGG 0.367528 2_1:-7667921 Msa0191930:CDS
CTCATACTAGCCACATTTGT+TGG 0.367569 2_1:-7666104 Msa0191930:CDS
ATGTTCTTGCGAAAACCATT+TGG 0.369501 2_1:-7666274 Msa0191930:CDS
CAGGAATTCTTGGAAAATCA+TGG 0.376419 2_1:+7665952 None:intergenic
GGTGCTCCAAGTGATACTTT+TGG 0.379124 2_1:+7666366 None:intergenic
TGATCCCTTCGCATTCTTCA+AGG 0.379772 2_1:-7667775 Msa0191930:intron
GACAAACTATCATAATATTC+TGG 0.380490 2_1:+7665897 None:intergenic
AGTTTATAAAGAATATTCAT+AGG 0.384167 2_1:+7665748 None:intergenic
ATGGGTAAAGGCGGAGGAGG+AGG 0.384192 2_1:-7667924 Msa0191930:CDS
TTGAAGAATGCGAAGGGATC+AGG 0.397880 2_1:+7667777 None:intergenic
TGTTCTTGCGAAAACCATTT+GGG 0.401291 2_1:-7666273 Msa0191930:CDS
CAATGGTTTATTTCTGGGGT+TGG 0.403013 2_1:-7666142 Msa0191930:CDS
CTGTCTACAACCGTTGGCTT+GGG 0.405458 2_1:-7666433 Msa0191930:CDS
TCAGTTTAAGGTTGAAGAAA+TGG 0.411609 2_1:-7665450 Msa0191930:three_prime_UTR
CAAAGAGTTGTTTAATTTGA+GGG 0.413350 2_1:+7667802 None:intergenic
CCAAAGAGTTGTTTAATTTG+AGG 0.414984 2_1:+7667801 None:intergenic
CAGACATAGGTACGCCTATA+GGG 0.434208 2_1:+7666396 None:intergenic
AATGTTTGGACACATATTGA+TGG 0.435853 2_1:+7665600 None:intergenic
CATTATTTGTCACTAGATAG+TGG 0.442233 2_1:-7665366 Msa0191930:three_prime_UTR
AAAATGGGTAAAGGCGGAGG+AGG 0.447267 2_1:-7667927 Msa0191930:CDS
CCTGTCTACAACCGTTGGCT+TGG 0.454505 2_1:-7666434 Msa0191930:CDS
CCAAGAAGAGATTGTGGTTG+AGG 0.459624 2_1:+7666495 None:intergenic
TATCAACGCCATCGACGCCT+TGG 0.464202 2_1:+7666336 None:intergenic
ACTACAGTCAACATGCGTTC+CGG 0.466049 2_1:-7665521 Msa0191930:three_prime_UTR
ACAGACATAGGTACGCCTAT+AGG 0.470286 2_1:+7666395 None:intergenic
GATTGCCCTTGATGTCTCAA+TGG 0.470604 2_1:-7666159 Msa0191930:CDS
AATAAACCATTGAGACATCA+AGG 0.477537 2_1:+7666153 None:intergenic
GTTTATAAAGAATATTCATA+GGG 0.479471 2_1:+7665749 None:intergenic
CATCGCTTCCAAGGCGTCGA+TGG 0.485169 2_1:-7666344 Msa0191930:CDS
GTAATAAGCTAATAAACAAA+TGG 0.485651 2_1:+7665658 None:intergenic
CAAGATTGTGACTGAGCTCA+TGG 0.487021 2_1:+7666468 None:intergenic
GACATGTGCTTCCATGTGAC+TGG 0.492679 2_1:+7666304 None:intergenic
ACACTATAAATCATGGAGTC+AGG 0.494471 2_1:-7665874 Msa0191930:CDS
TTTCTTAAACCGAAACCTCC+AGG 0.504284 2_1:-7666212 Msa0191930:CDS
GTCTCAATGGTTTATTTCTG+GGG 0.504698 2_1:-7666146 Msa0191930:CDS
CCAAGCCAACGGTTGTAGAC+AGG 0.507037 2_1:+7666434 None:intergenic
GATTAGCAGATTACTAGTAA+TGG 0.512849 2_1:-7665490 Msa0191930:three_prime_UTR
GTTTGTCACACTATAAATCA+TGG 0.516695 2_1:-7665881 Msa0191930:CDS
GGAGGAGGAGAAGATGAAGA+AGG 0.517452 2_1:-7667906 Msa0191930:CDS
CAACTCCTGTCTACAACCGT+TGG 0.519760 2_1:-7666439 Msa0191930:CDS
AATCTTCTCACATGGCATGT+AGG 0.525317 2_1:-7665990 Msa0191930:CDS
TTGGAGCACCATCGCTTCCA+AGG 0.530014 2_1:-7666353 Msa0191930:CDS
AACCTTCCAAAAGTATCACT+TGG 0.535024 2_1:-7666372 Msa0191930:CDS
ATACTAGCCACATTTGTTGG+TGG 0.545798 2_1:-7666101 Msa0191930:CDS
CTAGCCACATTTGTTGGTGG+AGG 0.551745 2_1:-7666098 Msa0191930:CDS
AGATGAAGAAGGTGTAATGA+TGG 0.551961 2_1:-7667895 Msa0191930:CDS
AACTAGAGAATGTACATGAT+TGG 0.552599 2_1:-7665553 Msa0191930:three_prime_UTR
TATATTATGGACAAAACAGT+TGG 0.553526 2_1:-7665840 Msa0191930:CDS
TGTTTATTAGCTTATTACAA+TGG 0.556839 2_1:-7665653 Msa0191930:three_prime_UTR
CAAGAGACATATTCTTACTA+CGG 0.561519 2_1:-7666020 Msa0191930:CDS
GGCTAGTATGAGATAAGCTA+AGG 0.565655 2_1:+7666115 None:intergenic
TAATTTGAGGGTATTTGGAG+AGG 0.567285 2_1:+7667814 None:intergenic
TTGATGAATTCCCAAATACC+TGG 0.573711 2_1:+7666194 None:intergenic
GAAATGAAGTGACCAGCCAT+TGG 0.573733 2_1:+7666068 None:intergenic
GGAGGAATGCATCCAATGGC+TGG 0.575417 2_1:-7666080 Msa0191930:CDS
TAATGGACAGAACATGATCA+TGG 0.577168 2_1:-7665473 Msa0191930:three_prime_UTR
GAAGAAGGTGTAATGATGGC+TGG 0.581573 2_1:-7667891 Msa0191930:CDS
CATCGACGCCTTGGAAGCGA+TGG 0.586947 2_1:+7666345 None:intergenic
ATCTGCTAATCTTTCCACTC+CGG 0.594440 2_1:+7665502 None:intergenic
GACCTTTGAATCTTCTCACA+TGG 0.598138 2_1:-7665998 Msa0191930:CDS
GCGAGAAGCATGAGGTTCAT+CGG 0.598370 2_1:+7667848 None:intergenic
TGCCATGTGAGAAGATTCAA+AGG 0.605214 2_1:+7665996 None:intergenic
CATTCCTCCACCAACAAATG+TGG 0.605926 2_1:+7666094 None:intergenic
ACATGTGCTTCCATGTGACT+GGG 0.609728 2_1:+7666305 None:intergenic
GTTTGGACACATATTGATGG+AGG 0.617544 2_1:+7665603 None:intergenic
TATGGATATCCCCAGTCACA+TGG 0.618891 2_1:-7666315 Msa0191930:CDS
CTCACCTTGAAGAATGCGAA+GGG 0.618939 2_1:+7667771 None:intergenic
TCCTGGAAACAAACTTCACA+AGG 0.620833 2_1:-7665934 Msa0191930:CDS
AGTCAACATGCGTTCCGGAG+TGG 0.622695 2_1:-7665516 Msa0191930:three_prime_UTR
GAAAATCATGGTGTTCATTG+TGG 0.623404 2_1:+7665964 None:intergenic
ACTCACCTTGAAGAATGCGA+AGG 0.629782 2_1:+7667770 None:intergenic
GGATAGCCAAGAAGAGATTG+TGG 0.634306 2_1:+7666489 None:intergenic
ATAAACCATTGAGACATCAA+GGG 0.649348 2_1:+7666154 None:intergenic
AAACAAACTTCACAAGGTGA+AGG 0.651727 2_1:-7665928 Msa0191930:CDS
TTTATAAAGAATATTCATAG+GGG 0.654681 2_1:+7665750 None:intergenic
TAGCGAAAATCCCAAGCCAA+CGG 0.655542 2_1:+7666423 None:intergenic
TGACGACGGCGAGAAGCATG+AGG 0.663294 2_1:+7667840 None:intergenic
ATGAATTCCCAAATACCTGG+AGG 0.666515 2_1:+7666197 None:intergenic
TGGTGGAGGAATGCATCCAA+TGG 0.675962 2_1:-7666084 Msa0191930:CDS
GGTTTCGGTTTAAGAAACAA+CGG 0.699596 2_1:+7666218 None:intergenic
CATGTGCTTCCATGTGACTG+GGG 0.710334 2_1:+7666306 None:intergenic
ATTTGGAGAGGATCTGACGA+CGG 0.745086 2_1:+7667826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAATGTATTAAAAAGA+TGG - chr2_1:7667859-7667878 Msa0191930:CDS 10.0%
!!! AAATTGATTTTGATATGTTT+GGG + chr2_1:7666376-7666395 None:intergenic 15.0%
!!! AAAATTGATTTTGATATGTT+TGG + chr2_1:7666377-7666396 None:intergenic 15.0%
!! TTTATAAAGAATATTCATAG+GGG + chr2_1:7667402-7667421 None:intergenic 15.0%
!! GTTTATAAAGAATATTCATA+GGG + chr2_1:7667403-7667422 None:intergenic 15.0%
!! AGTTTATAAAGAATATTCAT+AGG + chr2_1:7667404-7667423 None:intergenic 15.0%
!!! TCTTGAATTTCATTTTAATT+AGG - chr2_1:7667815-7667834 Msa0191930:CDS 15.0%
!! TATCAAACATGTACTAAAAA+CGG + chr2_1:7665445-7665464 None:intergenic 20.0%
!! AGAATCATATATTAGAATCA+CGG - chr2_1:7665481-7665500 Msa0191930:three_prime_UTR 20.0%
!!! ATGGTTTTTAGGAAAAATAT+TGG - chr2_1:7665580-7665599 Msa0191930:three_prime_UTR 20.0%
!!! TTTTTAGGAAAAATATTGGT+GGG - chr2_1:7665584-7665603 Msa0191930:three_prime_UTR 20.0%
!!! TTTTAGTTTAAAGTGAGTTT+AGG - chr2_1:7665991-7666010 Msa0191930:CDS 20.0%
!!! TTTAGTTTAAAGTGAGTTTA+GGG - chr2_1:7665992-7666011 Msa0191930:CDS 20.0%
!! AAGTATGTTGAACAATAATT+TGG - chr2_1:7666265-7666284 Msa0191930:CDS 20.0%
!! AGTATGTTGAACAATAATTT+GGG - chr2_1:7666266-7666285 Msa0191930:CDS 20.0%
!! AATAATTTGGGTGTAAAATT+TGG - chr2_1:7666278-7666297 Msa0191930:CDS 20.0%
!! TAGTGTAGATTATATTAGTT+TGG - chr2_1:7666423-7666442 Msa0191930:CDS 20.0%
!!! AGTGTAGATTATATTAGTTT+GGG - chr2_1:7666424-7666443 Msa0191930:CDS 20.0%
!! GGTTATTTACATGTATATTA+TGG - chr2_1:7667296-7667315 Msa0191930:intron 20.0%
!! GTAATAAGCTAATAAACAAA+TGG + chr2_1:7667494-7667513 None:intergenic 20.0%
!!! TGTTTATTAGCTTATTACAA+TGG - chr2_1:7667496-7667515 Msa0191930:intron 20.0%
!!! TTGTTTAATTTGAGGGTATT+TGG + chr2_1:7665343-7665362 None:intergenic 25.0%
!!! CAAAGAGTTGTTTAATTTGA+GGG + chr2_1:7665350-7665369 None:intergenic 25.0%
! TTAGTACATGTTTGATATCA+TGG - chr2_1:7665448-7665467 Msa0191930:three_prime_UTR 25.0%
!!! GTTTTTAGGAAAAATATTGG+TGG - chr2_1:7665583-7665602 Msa0191930:three_prime_UTR 25.0%
! AGTAAGTAACAACTTTGAAT+TGG - chr2_1:7665892-7665911 Msa0191930:CDS 25.0%
! AACAATTGAGAAAATTCCAA+TGG + chr2_1:7666030-7666049 None:intergenic 25.0%
!! GATTATATTAGTTTGGGATA+TGG - chr2_1:7666430-7666449 Msa0191930:CDS 25.0%
!! GTATTTTATGTGAATGTGTA+TGG - chr2_1:7666462-7666481 Msa0191930:CDS 25.0%
!! TTTTAGATCTGTATTAGATC+TGG - chr2_1:7666500-7666519 Msa0191930:CDS 25.0%
!!! TGTTACTGCTATTTTACTTA+AGG - chr2_1:7666582-7666601 Msa0191930:CDS 25.0%
! GACAAACTATCATAATATTC+TGG + chr2_1:7667255-7667274 None:intergenic 25.0%
! TATATTATGGACAAAACAGT+TGG - chr2_1:7667309-7667328 Msa0191930:intron 25.0%
! ACTTTCTTTGTTGAAATGTT+TGG + chr2_1:7667566-7667585 None:intergenic 25.0%
!! CCAAAGAGTTGTTTAATTTG+AGG + chr2_1:7665351-7665370 None:intergenic 30.0%
CCTCAAATTAAACAACTCTT+TGG - chr2_1:7665348-7665367 Msa0191930:three_prime_UTR 30.0%
AGTAACATTAGAGATCTAGA+TGG + chr2_1:7665414-7665433 None:intergenic 30.0%
CTAAAAACCATTGTTACACA+TGG + chr2_1:7665571-7665590 None:intergenic 30.0%
! CATGTGTAACAATGGTTTTT+AGG - chr2_1:7665569-7665588 Msa0191930:three_prime_UTR 30.0%
TAAAGATGTGAGATAACTTG+TGG - chr2_1:7665777-7665796 Msa0191930:three_prime_UTR 30.0%
! AAAGATGTGAGATAACTTGT+GGG - chr2_1:7665778-7665797 Msa0191930:exon 30.0%
TAAATCTGATGTGAGATTGT+AGG - chr2_1:7665859-7665878 Msa0191930:CDS 30.0%
AAATCTGATGTGAGATTGTA+GGG - chr2_1:7665860-7665879 Msa0191930:CDS 30.0%
! TTTTTCTCTCTCATGTTTGA+TGG - chr2_1:7666078-7666097 Msa0191930:CDS 30.0%
ATGTTGTATAAGATTTGCAG+TGG - chr2_1:7666184-7666203 Msa0191930:CDS 30.0%
ATGTGAATGTGTATGGTTTA+TGG - chr2_1:7666469-7666488 Msa0191930:CDS 30.0%
! CTATTTTACTTAAGGATGCA+GGG - chr2_1:7666590-7666609 Msa0191930:CDS 30.0%
!!! CTTCTAGTAGCCTATTTTTT+TGG - chr2_1:7666625-7666644 Msa0191930:intron 30.0%
ATAAACCATTGAGACATCAA+GGG + chr2_1:7666998-7667017 None:intergenic 30.0%
AATAAACCATTGAGACATCA+AGG + chr2_1:7666999-7667018 None:intergenic 30.0%
ATGTCTCAATGGTTTATTTC+TGG - chr2_1:7667001-7667020 Msa0191930:intron 30.0%
! TGTCTCAATGGTTTATTTCT+GGG - chr2_1:7667002-7667021 Msa0191930:intron 30.0%
TAAGAATATGTCTCTTGTTC+AGG + chr2_1:7667126-7667145 None:intergenic 30.0%
CAAGAGACATATTCTTACTA+CGG - chr2_1:7667129-7667148 Msa0191930:intron 30.0%
GTTTGTCACACTATAAATCA+TGG - chr2_1:7667268-7667287 Msa0191930:intron 30.0%
TTTCTCTTCATTCTGCTATA+AGG + chr2_1:7667336-7667355 None:intergenic 30.0%
! TCTATTCATTCAGTTTTGCT+TGG + chr2_1:7667360-7667379 None:intergenic 30.0%
! ACAATGGTGATGAATGTTAT+TGG - chr2_1:7667512-7667531 Msa0191930:intron 30.0%
AATGTTTGGACACATATTGA+TGG + chr2_1:7667552-7667571 None:intergenic 30.0%
AACTAGAGAATGTACATGAT+TGG - chr2_1:7667596-7667615 Msa0191930:intron 30.0%
GATTAGCAGATTACTAGTAA+TGG - chr2_1:7667659-7667678 Msa0191930:intron 30.0%
! TCAGTTTAAGGTTGAAGAAA+TGG - chr2_1:7667699-7667718 Msa0191930:intron 30.0%
CATTATTTGTCACTAGATAG+TGG - chr2_1:7667783-7667802 Msa0191930:CDS 30.0%
! AGATGAAGAAGGTGTAATGA+TGG - chr2_1:7665254-7665273 Msa0191930:three_prime_UTR 35.0%
!! TAATTTGAGGGTATTTGGAG+AGG + chr2_1:7665338-7665357 None:intergenic 35.0%
!! TCAAGGTGTCAAAAGGTTTT+GGG + chr2_1:7665611-7665630 None:intergenic 35.0%
GATATGTTGCTGACATAGAT+AGG - chr2_1:7665662-7665681 Msa0191930:three_prime_UTR 35.0%
ACACCATTTGTGTGAAAAGT+TGG - chr2_1:7665827-7665846 Msa0191930:CDS 35.0%
! TCACCAACTTTTCACACAAA+TGG + chr2_1:7665833-7665852 None:intergenic 35.0%
! AAGTTGTTACTTACTTCTCG+TGG + chr2_1:7665887-7665906 None:intergenic 35.0%
AAAAGAGGAGATTGAGTTTG+TGG + chr2_1:7666062-7666081 None:intergenic 35.0%
CAAACATGAGAGAGAAAAAG+AGG + chr2_1:7666077-7666096 None:intergenic 35.0%
TCCAATGTTGACAATTCCAA+TGG - chr2_1:7666214-7666233 Msa0191930:CDS 35.0%
CAAGCTGAAATCAATTCTAG+AGG - chr2_1:7666326-7666345 Msa0191930:CDS 35.0%
ATCTACACTAGTGACAAGTT+TGG + chr2_1:7666413-7666432 None:intergenic 35.0%
!! GCTATTTTACTTAAGGATGC+AGG - chr2_1:7666589-7666608 Msa0191930:CDS 35.0%
GAGGAATGAGCCAAAAAAAT+AGG + chr2_1:7666638-7666657 None:intergenic 35.0%
! ATTTTCGCTAATCTCCCTAT+AGG - chr2_1:7666739-7666758 Msa0191930:intron 35.0%
!! TTTTGGAAGGTTACAGACAT+AGG + chr2_1:7666769-7666788 None:intergenic 35.0%
!! AACCTTCCAAAAGTATCACT+TGG - chr2_1:7666777-7666796 Msa0191930:intron 35.0%
ATGTTCTTGCGAAAACCATT+TGG - chr2_1:7666875-7666894 Msa0191930:intron 35.0%
TGTTCTTGCGAAAACCATTT+GGG - chr2_1:7666876-7666895 Msa0191930:intron 35.0%
TAGCTGTAAAAAGACCCAAA+TGG + chr2_1:7666893-7666912 None:intergenic 35.0%
GGTTTCGGTTTAAGAAACAA+CGG + chr2_1:7666934-7666953 None:intergenic 35.0%
TTGATGAATTCCCAAATACC+TGG + chr2_1:7666958-7666977 None:intergenic 35.0%
!! GTCTCAATGGTTTATTTCTG+GGG - chr2_1:7667003-7667022 Msa0191930:intron 35.0%
GAAAATCATGGTGTTCATTG+TGG + chr2_1:7667188-7667207 None:intergenic 35.0%
CAGGAATTCTTGGAAAATCA+TGG + chr2_1:7667200-7667219 None:intergenic 35.0%
! CATGATTTTCCAAGAATTCC+TGG - chr2_1:7667198-7667217 Msa0191930:intron 35.0%
! AGTTTGTTTCCAGGAATTCT+TGG + chr2_1:7667210-7667229 None:intergenic 35.0%
AAACAAACTTCACAAGGTGA+AGG - chr2_1:7667221-7667240 Msa0191930:intron 35.0%
ACACTATAAATCATGGAGTC+AGG - chr2_1:7667275-7667294 Msa0191930:intron 35.0%
!! AAACAAATGGCTTGCTATCT+GGG + chr2_1:7667481-7667500 None:intergenic 35.0%
!! TAAACAAATGGCTTGCTATC+TGG + chr2_1:7667482-7667501 None:intergenic 35.0%
! TAATGGACAGAACATGATCA+TGG - chr2_1:7667676-7667695 Msa0191930:intron 35.0%
ACATGATCATGGTCAGTTTA+AGG - chr2_1:7667687-7667706 Msa0191930:intron 35.0%
! TGATGGCTGGTGATTTCTTT+TGG - chr2_1:7665271-7665290 Msa0191930:three_prime_UTR 40.0%
ATTGTTACACATGGCATTCG+GGG + chr2_1:7665562-7665581 None:intergenic 40.0%
CATTGTTACACATGGCATTC+GGG + chr2_1:7665563-7665582 None:intergenic 40.0%
CCATTGTTACACATGGCATT+CGG + chr2_1:7665564-7665583 None:intergenic 40.0%
!! CAAGGTGTCAAAAGGTTTTG+GGG + chr2_1:7665610-7665629 None:intergenic 40.0%
!! CTCAAGGTGTCAAAAGGTTT+TGG + chr2_1:7665612-7665631 None:intergenic 40.0%
GGGGTCATCTCTAAATCTAA+TGG + chr2_1:7665923-7665942 None:intergenic 40.0%
ATAGAACCAATGACACACTG+GGG + chr2_1:7665942-7665961 None:intergenic 40.0%
CATAGAACCAATGACACACT+GGG + chr2_1:7665943-7665962 None:intergenic 40.0%
TCATAGAACCAATGACACAC+TGG + chr2_1:7665944-7665963 None:intergenic 40.0%
! AGGGAATTGATTCTCTCCAT+TGG - chr2_1:7666011-7666030 Msa0191930:CDS 40.0%
AATCCATTTCCGTGAGCTTA+AGG - chr2_1:7666150-7666169 Msa0191930:CDS 40.0%
TTACCTTAAGCTCACGGAAA+TGG + chr2_1:7666156-7666175 None:intergenic 40.0%
AGCATGTTACCTTAAGCTCA+CGG + chr2_1:7666162-7666181 None:intergenic 40.0%
GCCATTGGAATTGTCAACAT+TGG + chr2_1:7666218-7666237 None:intergenic 40.0%
AAGCTGCAAATCTTAGCCAT+TGG + chr2_1:7666233-7666252 None:intergenic 40.0%
! TTTTACTTAAGGATGCAGGG+TGG - chr2_1:7666593-7666612 Msa0191930:CDS 40.0%
! CTCCAAGTGATACTTTTGGA+AGG + chr2_1:7666782-7666801 None:intergenic 40.0%
TTTCTTAAACCGAAACCTCC+AGG - chr2_1:7666937-7666956 Msa0191930:intron 40.0%
ATGAATTCCCAAATACCTGG+AGG + chr2_1:7666955-7666974 None:intergenic 40.0%
!! CAATGGTTTATTTCTGGGGT+TGG - chr2_1:7667007-7667026 Msa0191930:intron 40.0%
GGCTAGTATGAGATAAGCTA+AGG + chr2_1:7667037-7667056 None:intergenic 40.0%
CTCATACTAGCCACATTTGT+TGG - chr2_1:7667045-7667064 Msa0191930:intron 40.0%
! ATACTAGCCACATTTGTTGG+TGG - chr2_1:7667048-7667067 Msa0191930:intron 40.0%
GACCTTTGAATCTTCTCACA+TGG - chr2_1:7667151-7667170 Msa0191930:intron 40.0%
TGCCATGTGAGAAGATTCAA+AGG + chr2_1:7667156-7667175 None:intergenic 40.0%
AATCTTCTCACATGGCATGT+AGG - chr2_1:7667159-7667178 Msa0191930:intron 40.0%
TCCTGGAAACAAACTTCACA+AGG - chr2_1:7667215-7667234 Msa0191930:intron 40.0%
! ACCTTGTGAAGTTTGTTTCC+AGG + chr2_1:7667219-7667238 None:intergenic 40.0%
GTTTGGACACATATTGATGG+AGG + chr2_1:7667549-7667568 None:intergenic 40.0%
ATCTGCTAATCTTTCCACTC+CGG + chr2_1:7667650-7667669 None:intergenic 40.0%
! GAAGAAGGTGTAATGATGGC+TGG - chr2_1:7665258-7665277 Msa0191930:three_prime_UTR 45.0%
ATTTGGAGAGGATCTGACGA+CGG + chr2_1:7665326-7665345 None:intergenic 45.0%
TTGAAGAATGCGAAGGGATC+AGG + chr2_1:7665375-7665394 None:intergenic 45.0%
TGATCCCTTCGCATTCTTCA+AGG - chr2_1:7665374-7665393 Msa0191930:three_prime_UTR 45.0%
CTCACCTTGAAGAATGCGAA+GGG + chr2_1:7665381-7665400 None:intergenic 45.0%
ACTCACCTTGAAGAATGCGA+AGG + chr2_1:7665382-7665401 None:intergenic 45.0%
CACATGTTTGGTACCACGAT+GGG + chr2_1:7665531-7665550 None:intergenic 45.0%
CCGAATGCCATGTGTAACAA+TGG - chr2_1:7665561-7665580 Msa0191930:three_prime_UTR 45.0%
! CTCTCTCTCAAGGTGTCAAA+AGG + chr2_1:7665618-7665637 None:intergenic 45.0%
!! CGGAAATGGATTGTCAACGT+TGG + chr2_1:7666142-7666161 None:intergenic 45.0%
CCAAGAAGAGATTGTGGTTG+AGG + chr2_1:7666657-7666676 None:intergenic 45.0%
CCTCAACCACAATCTCTTCT+TGG - chr2_1:7666654-7666673 Msa0191930:intron 45.0%
GGATAGCCAAGAAGAGATTG+TGG + chr2_1:7666663-7666682 None:intergenic 45.0%
CAAGATTGTGACTGAGCTCA+TGG + chr2_1:7666684-7666703 None:intergenic 45.0%
TAGCGAAAATCCCAAGCCAA+CGG + chr2_1:7666729-7666748 None:intergenic 45.0%
CAGACATAGGTACGCCTATA+GGG + chr2_1:7666756-7666775 None:intergenic 45.0%
ACAGACATAGGTACGCCTAT+AGG + chr2_1:7666757-7666776 None:intergenic 45.0%
! GGTGCTCCAAGTGATACTTT+TGG + chr2_1:7666786-7666805 None:intergenic 45.0%
TATGGATATCCCCAGTCACA+TGG - chr2_1:7666834-7666853 Msa0191930:intron 45.0%
ACATGTGCTTCCATGTGACT+GGG + chr2_1:7666847-7666866 None:intergenic 45.0%
AACCGAAACCTCCAGGTATT+TGG - chr2_1:7666944-7666963 Msa0191930:intron 45.0%
ACCGAAACCTCCAGGTATTT+GGG - chr2_1:7666945-7666964 Msa0191930:intron 45.0%
TCCCAAATACCTGGAGGTTT+CGG + chr2_1:7666949-7666968 None:intergenic 45.0%
GATTGCCCTTGATGTCTCAA+TGG - chr2_1:7666990-7667009 Msa0191930:intron 45.0%
CATTCCTCCACCAACAAATG+TGG + chr2_1:7667058-7667077 None:intergenic 45.0%
GAAATGAAGTGACCAGCCAT+TGG + chr2_1:7667084-7667103 None:intergenic 45.0%
ACTACAGTCAACATGCGTTC+CGG - chr2_1:7667628-7667647 Msa0191930:intron 45.0%
GGAGGAGGAGAAGATGAAGA+AGG - chr2_1:7665243-7665262 Msa0191930:three_prime_UTR 50.0%
! GCGAGAAGCATGAGGTTCAT+CGG + chr2_1:7665304-7665323 None:intergenic 50.0%
TTGCAAATCACAGCCCATCG+TGG - chr2_1:7665515-7665534 Msa0191930:three_prime_UTR 50.0%
CCACATGTTTGGTACCACGA+TGG + chr2_1:7665532-7665551 None:intergenic 50.0%
CCATCGTGGTACCAAACATG+TGG - chr2_1:7665529-7665548 Msa0191930:three_prime_UTR 50.0%
GGGGTCAATCTCCACATGTT+TGG + chr2_1:7665543-7665562 None:intergenic 50.0%
ACACCTTGAGAGAGAGAGAG+AGG - chr2_1:7665622-7665641 Msa0191930:three_prime_UTR 50.0%
ATGCCTCTCTCTCTCTCTCA+AGG + chr2_1:7665628-7665647 None:intergenic 50.0%
AGATGACCCCAGTGTGTCAT+TGG - chr2_1:7665933-7665952 Msa0191930:CDS 50.0%
CAACTCCTGTCTACAACCGT+TGG - chr2_1:7666710-7666729 Msa0191930:intron 50.0%
!! CTGTCTACAACCGTTGGCTT+GGG - chr2_1:7666716-7666735 Msa0191930:intron 50.0%
CATGTGCTTCCATGTGACTG+GGG + chr2_1:7666846-7666865 None:intergenic 50.0%
GACATGTGCTTCCATGTGAC+TGG + chr2_1:7666848-7666867 None:intergenic 50.0%
! CTAGCCACATTTGTTGGTGG+AGG - chr2_1:7667051-7667070 Msa0191930:intron 50.0%
TGGTGGAGGAATGCATCCAA+TGG - chr2_1:7667065-7667084 Msa0191930:intron 50.0%
CCAAGCCAACGGTTGTAGAC+AGG + chr2_1:7666718-7666737 None:intergenic 55.0%
! CCTGTCTACAACCGTTGGCT+TGG - chr2_1:7666715-7666734 Msa0191930:intron 55.0%
! TTGGAGCACCATCGCTTCCA+AGG - chr2_1:7666796-7666815 Msa0191930:intron 55.0%
TATCAACGCCATCGACGCCT+TGG + chr2_1:7666816-7666835 None:intergenic 55.0%
!! AGGCGTCGATGGCGTTGATA+TGG - chr2_1:7666816-7666835 Msa0191930:intron 55.0%
GGAGGAATGCATCCAATGGC+TGG - chr2_1:7667069-7667088 Msa0191930:intron 55.0%
AGTCAACATGCGTTCCGGAG+TGG - chr2_1:7667633-7667652 Msa0191930:intron 55.0%
ATGGGTAAAGGCGGAGGAGG+AGG - chr2_1:7665225-7665244 Msa0191930:three_prime_UTR 60.0%
! TGACGACGGCGAGAAGCATG+AGG + chr2_1:7665312-7665331 None:intergenic 60.0%
CATCGACGCCTTGGAAGCGA+TGG + chr2_1:7666807-7666826 None:intergenic 60.0%
!! CATCGCTTCCAAGGCGTCGA+TGG - chr2_1:7666805-7666824 Msa0191930:intron 60.0%
GGTAAAGGCGGAGGAGGAGG+AGG - chr2_1:7665228-7665247 Msa0191930:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 7665225 7667946 7665225 ID=Msa0191930;Name=Msa0191930
chr2_1 mRNA 7665225 7667946 7665225 ID=Msa0191930-mRNA-1;Parent=Msa0191930;Name=Msa0191930-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|572|329
chr2_1 exon 7665225 7666615 7665225 ID=Msa0191930-mRNA-1:exon:3492;Parent=Msa0191930-mRNA-1
chr2_1 exon 7667776 7667946 7667776 ID=Msa0191930-mRNA-1:exon:3491;Parent=Msa0191930-mRNA-1
chr2_1 CDS 7667776 7667946 7667776 ID=Msa0191930-mRNA-1:cds;Parent=Msa0191930-mRNA-1
chr2_1 CDS 7665797 7666615 7665797 ID=Msa0191930-mRNA-1:cds;Parent=Msa0191930-mRNA-1
chr2_1 three_prime_UTR 7665225 7665796 7665225 ID=Msa0191930-mRNA-1:three_prime_utr;Parent=Msa0191930-mRNA-1
Gene Sequence

>Msa0191930

ATGGGTAAAGGCGGAGGAGGAGGAGGAGAAGATGAAGAAGGTGTAATGATGGCTGGTGATTTCTTTTGGTCATATACCGATGAACCTCATGCTTCTCGCCGTCGTCAGATCCTCTCCAAATACCCTCAAATTAAACAACTCTTTGGTCCTGATCCCTTCGCATTCTTCAAGATTAGTGTTGTTGTTCTGCTTCAACTTGTTACTGCTATTTTACTTAAGGATGCAGGGTGGCTGAAGATACTTCTAGTAGCCTATTTTTTTGGCTCATTCCTCAACCACAATCTCTTCTTGGCTATCCATGAGCTCAGTCACAATCTTGCTTTTTCAACTCCTGTCTACAACCGTTGGCTTGGGATTTTCGCTAATCTCCCTATAGGCGTACCTATGTCTGTAACCTTCCAAAAGTATCACTTGGAGCACCATCGCTTCCAAGGCGTCGATGGCGTTGATATGGATATCCCCAGTCACATGGAAGCACATGTCGTAACAAATGTTCTTGCGAAAACCATTTGGGTCTTTTTACAGCTATTCTTTTATGCATTTCGACCGTTGTTTCTTAAACCGAAACCTCCAGGTATTTGGGAATTCATCAACTTCTCTGTTCAGATTGCCCTTGATGTCTCAATGGTTTATTTCTGGGGTTGGAAATCCTTAGCTTATCTCATACTAGCCACATTTGTTGGTGGAGGAATGCATCCAATGGCTGGTCACTTCATTTCAGAACATTATGTATTCAATCCTGAACAAGAGACATATTCTTACTACGGACCTTTGAATCTTCTCACATGGCATGTAGGTTACCACAATGAACACCATGATTTTCCAAGAATTCCTGGAAACAAACTTCACAAGGTGAAGGAGATTGCACCAGAATATTATGATAGTTTGTCACACTATAAATCATGGAGTCAGGTTATTTACATGTATATTATGGACAAAACAGTTGGACCTTATAGCAGAATGAAGAGAAAGCCAAGCAAAACTGAATGA
Protein sequence

>Msa0191930

MGKGGGGGGEDEEGVMMAGDFFWSYTDEPHASRRRQILSKYPQIKQLFGPDPFAFFKISVVVLLQLVTAILLKDAGWLKILLVAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGVDMDIPSHMEAHVVTNVLAKTIWVFLQLFFYAFRPLFLKPKPPGIWEFINFSVQIALDVSMVYFWGWKSLAYLILATFVGGGMHPMAGHFISEHYVFNPEQETYSYYGPLNLLTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDSLSHYKSWSQVIYMYIMDKTVGPYSRMKRKPSKTE*