AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0308260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308260 MtrunA17_Chr2g0281851 97.568 329 7 1 1 329 27 354 0.0 660
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308260 AT4G04930.1 81.682 333 56 2 1 329 1 332 0.0 567

Find 102 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCTCAATGGTTTATTTC+TGG 0.042011 2_4:-2457792 Msa0308260:CDS
AACCGAAACCTCCAGGTATT+TGG 0.110935 2_4:-2457849 Msa0308260:CDS
TAAACAAATGGCTTGCTATC+TGG 0.171894 2_4:+2457315 None:intergenic
ACATGATCATGGTCAGTTTA+AGG 0.208576 2_4:-2457107 Msa0308260:three_prime_UTR
GGTTATTTACATGTATATTA+TGG 0.220265 2_4:-2457497 Msa0308260:CDS
TTGTTTAATTTGAGGGTATT+TGG 0.221781 2_4:+2459453 None:intergenic
TTTCTCTTCATTCTGCTATA+AGG 0.250507 2_4:+2457460 None:intergenic
CCTCAACCACAATCTCTTCT+TGG 0.268270 2_4:-2458139 Msa0308260:CDS
ACAATGGTGATGAATGTTAT+TGG 0.292521 2_4:-2457282 Msa0308260:three_prime_UTR
TGTCTCAATGGTTTATTTCT+GGG 0.295906 2_4:-2457791 Msa0308260:CDS
TGATGGCTGGTGATTTCTTT+TGG 0.300981 2_4:-2459522 Msa0308260:CDS
ACTTTCTTTGTTGAAATGTT+TGG 0.306805 2_4:+2457231 None:intergenic
AGGCGTCGATGGCGTTGATA+TGG 0.307309 2_4:-2457977 Msa0308260:CDS
ACCTTGTGAAGTTTGTTTCC+AGG 0.308162 2_4:+2457577 None:intergenic
TCCCAAATACCTGGAGGTTT+CGG 0.315484 2_4:+2457847 None:intergenic
TAAGAATATGTCTCTTGTTC+AGG 0.318858 2_4:+2457670 None:intergenic
ACCGAAACCTCCAGGTATTT+GGG 0.325860 2_4:-2457848 Msa0308260:CDS
CCTCAAATTAAACAACTCTT+TGG 0.348261 2_4:-2459445 Msa0308260:CDS
AGTTTGTTTCCAGGAATTCT+TGG 0.349785 2_4:+2457586 None:intergenic
AAACAAATGGCTTGCTATCT+GGG 0.353317 2_4:+2457316 None:intergenic
GGTAAAGGCGGAGGAGGAGG+AGG 0.367528 2_4:-2459565 Msa0308260:CDS
CTCATACTAGCCACATTTGT+TGG 0.367569 2_4:-2457748 Msa0308260:CDS
ATGTTCTTGCGAAAACCATT+TGG 0.369501 2_4:-2457918 Msa0308260:CDS
CAGGAATTCTTGGAAAATCA+TGG 0.376419 2_4:+2457596 None:intergenic
GGTGCTCCAAGTGATACTTT+TGG 0.379124 2_4:+2458010 None:intergenic
TGATCCCTTCGCATTCTTCA+AGG 0.379772 2_4:-2459419 Msa0308260:intron
GACAAACTATCATAATATTC+TGG 0.380490 2_4:+2457541 None:intergenic
AGTTTATAAAGAATATTCAT+AGG 0.384167 2_4:+2457392 None:intergenic
ATGGGTAAAGGCGGAGGAGG+AGG 0.384192 2_4:-2459568 Msa0308260:CDS
TTGAAGAATGCGAAGGGATC+AGG 0.397880 2_4:+2459421 None:intergenic
TGTTCTTGCGAAAACCATTT+GGG 0.401291 2_4:-2457917 Msa0308260:CDS
CAATGGTTTATTTCTGGGGT+TGG 0.403013 2_4:-2457786 Msa0308260:CDS
CTGTCTACAACCGTTGGCTT+GGG 0.405458 2_4:-2458077 Msa0308260:CDS
TCAGTTTAAGGTTGAAGAAA+TGG 0.411609 2_4:-2457095 Msa0308260:three_prime_UTR
CAAAGAGTTGTTTAATTTGA+GGG 0.413350 2_4:+2459446 None:intergenic
CCAAAGAGTTGTTTAATTTG+AGG 0.414984 2_4:+2459445 None:intergenic
CAGACATAGGTACGCCTATA+GGG 0.434208 2_4:+2458040 None:intergenic
AATGTTTGGACACATATTGA+TGG 0.435853 2_4:+2457245 None:intergenic
CATTATTTGTCACTAGATAG+TGG 0.442233 2_4:-2457011 Msa0308260:three_prime_UTR
AAAATGGGTAAAGGCGGAGG+AGG 0.447267 2_4:-2459571 Msa0308260:CDS
CCTGTCTACAACCGTTGGCT+TGG 0.454505 2_4:-2458078 Msa0308260:CDS
CCAAGAAGAGATTGTGGTTG+AGG 0.459624 2_4:+2458139 None:intergenic
TATCAACGCCATCGACGCCT+TGG 0.464202 2_4:+2457980 None:intergenic
ACTACAGTCAACATGCGTTC+CGG 0.466049 2_4:-2457166 Msa0308260:three_prime_UTR
ACAGACATAGGTACGCCTAT+AGG 0.470286 2_4:+2458039 None:intergenic
GATTGCCCTTGATGTCTCAA+TGG 0.470604 2_4:-2457803 Msa0308260:CDS
AATAAACCATTGAGACATCA+AGG 0.477537 2_4:+2457797 None:intergenic
GTTTATAAAGAATATTCATA+GGG 0.479471 2_4:+2457393 None:intergenic
CATCGCTTCCAAGGCGTCGA+TGG 0.485169 2_4:-2457988 Msa0308260:CDS
GTAATAAGCTAATAAACAAA+TGG 0.485651 2_4:+2457303 None:intergenic
CAAGATTGTGACTGAGCTCA+TGG 0.487021 2_4:+2458112 None:intergenic
GACATGTGCTTCCATGTGAC+TGG 0.492679 2_4:+2457948 None:intergenic
ACACTATAAATCATGGAGTC+AGG 0.494471 2_4:-2457518 Msa0308260:CDS
TTTCTTAAACCGAAACCTCC+AGG 0.504284 2_4:-2457856 Msa0308260:CDS
GTCTCAATGGTTTATTTCTG+GGG 0.504698 2_4:-2457790 Msa0308260:CDS
CCAAGCCAACGGTTGTAGAC+AGG 0.507037 2_4:+2458078 None:intergenic
GATTAGCAGATTACTAGTAA+TGG 0.512849 2_4:-2457135 Msa0308260:three_prime_UTR
GTTTGTCACACTATAAATCA+TGG 0.516695 2_4:-2457525 Msa0308260:CDS
GGAGGAGGAGAAGATGAAGA+AGG 0.517452 2_4:-2459550 Msa0308260:CDS
CAACTCCTGTCTACAACCGT+TGG 0.519760 2_4:-2458083 Msa0308260:CDS
AATCTTCTCACATGGCATGT+AGG 0.525317 2_4:-2457634 Msa0308260:CDS
TTGGAGCACCATCGCTTCCA+AGG 0.530014 2_4:-2457997 Msa0308260:CDS
AACCTTCCAAAAGTATCACT+TGG 0.535024 2_4:-2458016 Msa0308260:CDS
ATACTAGCCACATTTGTTGG+TGG 0.545798 2_4:-2457745 Msa0308260:CDS
CTAGCCACATTTGTTGGTGG+AGG 0.551745 2_4:-2457742 Msa0308260:CDS
AGATGAAGAAGGTGTAATGA+TGG 0.551961 2_4:-2459539 Msa0308260:CDS
AACTAGAGAATGTACATGAT+TGG 0.552599 2_4:-2457198 Msa0308260:three_prime_UTR
TATATTATGGACAAAACAGT+TGG 0.553526 2_4:-2457484 Msa0308260:CDS
TGTTTATTAGCTTATTACAA+TGG 0.556839 2_4:-2457298 Msa0308260:three_prime_UTR
CAAGAGACATATTCTTACTA+CGG 0.561519 2_4:-2457664 Msa0308260:CDS
GGCTAGTATGAGATAAGCTA+AGG 0.565655 2_4:+2457759 None:intergenic
TAATTTGAGGGTATTTGGAG+AGG 0.567285 2_4:+2459458 None:intergenic
TTGATGAATTCCCAAATACC+TGG 0.573711 2_4:+2457838 None:intergenic
GAAATGAAGTGACCAGCCAT+TGG 0.573733 2_4:+2457712 None:intergenic
GGAGGAATGCATCCAATGGC+TGG 0.575417 2_4:-2457724 Msa0308260:CDS
TAATGGACAGAACATGATCA+TGG 0.577168 2_4:-2457118 Msa0308260:three_prime_UTR
GAAGAAGGTGTAATGATGGC+TGG 0.581573 2_4:-2459535 Msa0308260:CDS
CATCGACGCCTTGGAAGCGA+TGG 0.586947 2_4:+2457989 None:intergenic
ATCTGCTAATCTTTCCACTC+CGG 0.594440 2_4:+2457147 None:intergenic
GACCTTTGAATCTTCTCACA+TGG 0.598138 2_4:-2457642 Msa0308260:CDS
GCGAGAAGCATGAGGTTCAT+CGG 0.598370 2_4:+2459492 None:intergenic
TGCCATGTGAGAAGATTCAA+AGG 0.605214 2_4:+2457640 None:intergenic
CATTCCTCCACCAACAAATG+TGG 0.605926 2_4:+2457738 None:intergenic
ACATGTGCTTCCATGTGACT+GGG 0.609728 2_4:+2457949 None:intergenic
GTTTGGACACATATTGATGG+AGG 0.617544 2_4:+2457248 None:intergenic
TATGGATATCCCCAGTCACA+TGG 0.618891 2_4:-2457959 Msa0308260:CDS
CTCACCTTGAAGAATGCGAA+GGG 0.618939 2_4:+2459415 None:intergenic
TCCTGGAAACAAACTTCACA+AGG 0.620833 2_4:-2457578 Msa0308260:CDS
AGTCAACATGCGTTCCGGAG+TGG 0.622695 2_4:-2457161 Msa0308260:three_prime_UTR
GAAAATCATGGTGTTCATTG+TGG 0.623404 2_4:+2457608 None:intergenic
ACTCACCTTGAAGAATGCGA+AGG 0.629782 2_4:+2459414 None:intergenic
GGATAGCCAAGAAGAGATTG+TGG 0.634306 2_4:+2458133 None:intergenic
ATAAACCATTGAGACATCAA+GGG 0.649348 2_4:+2457798 None:intergenic
AAACAAACTTCACAAGGTGA+AGG 0.651727 2_4:-2457572 Msa0308260:CDS
TTTATAAAGAATATTCATAG+GGG 0.654681 2_4:+2457394 None:intergenic
TAGCGAAAATCCCAAGCCAA+CGG 0.655542 2_4:+2458067 None:intergenic
TGACGACGGCGAGAAGCATG+AGG 0.663294 2_4:+2459484 None:intergenic
ATGAATTCCCAAATACCTGG+AGG 0.666515 2_4:+2457841 None:intergenic
TGGTGGAGGAATGCATCCAA+TGG 0.675962 2_4:-2457728 Msa0308260:CDS
GGTTTCGGTTTAAGAAACAA+CGG 0.699596 2_4:+2457862 None:intergenic
CATGTGCTTCCATGTGACTG+GGG 0.710334 2_4:+2457950 None:intergenic
ATTTGGAGAGGATCTGACGA+CGG 0.745086 2_4:+2459470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAATGTATTAAAAAGA+TGG - chr2_4:2459503-2459522 Msa0308260:CDS 10.0%
!!! AAATTGATTTTGATATGTTT+GGG + chr2_4:2458021-2458040 None:intergenic 15.0%
!!! AAAATTGATTTTGATATGTT+TGG + chr2_4:2458022-2458041 None:intergenic 15.0%
!! TTTATAAAGAATATTCATAG+GGG + chr2_4:2459047-2459066 None:intergenic 15.0%
!! GTTTATAAAGAATATTCATA+GGG + chr2_4:2459048-2459067 None:intergenic 15.0%
!! AGTTTATAAAGAATATTCAT+AGG + chr2_4:2459049-2459068 None:intergenic 15.0%
!!! TCTTGAATTTCATTTTAATT+AGG - chr2_4:2459459-2459478 Msa0308260:CDS 15.0%
!! TATCAAACATGTACTAAAAA+CGG + chr2_4:2457090-2457109 None:intergenic 20.0%
!! AGAATCATATATTAGAATCA+CGG - chr2_4:2457126-2457145 Msa0308260:three_prime_UTR 20.0%
!!! ATGGTTTTTAGGAAAAATAT+TGG - chr2_4:2457225-2457244 Msa0308260:three_prime_UTR 20.0%
!!! TTTTTAGGAAAAATATTGGT+GGG - chr2_4:2457229-2457248 Msa0308260:three_prime_UTR 20.0%
!!! TTTTAGTTTAAAGTGAGTTT+AGG - chr2_4:2457636-2457655 Msa0308260:CDS 20.0%
!!! TTTAGTTTAAAGTGAGTTTA+GGG - chr2_4:2457637-2457656 Msa0308260:CDS 20.0%
!! AAGTATGTTGAACAATAATT+TGG - chr2_4:2457910-2457929 Msa0308260:CDS 20.0%
!! AGTATGTTGAACAATAATTT+GGG - chr2_4:2457911-2457930 Msa0308260:CDS 20.0%
!! AATAATTTGGGTGTAAAATT+TGG - chr2_4:2457923-2457942 Msa0308260:CDS 20.0%
!! TAGTGTAGATTATATTAGTT+TGG - chr2_4:2458068-2458087 Msa0308260:CDS 20.0%
!!! AGTGTAGATTATATTAGTTT+GGG - chr2_4:2458069-2458088 Msa0308260:CDS 20.0%
!! GGTTATTTACATGTATATTA+TGG - chr2_4:2458941-2458960 Msa0308260:intron 20.0%
!! GTAATAAGCTAATAAACAAA+TGG + chr2_4:2459138-2459157 None:intergenic 20.0%
!!! TGTTTATTAGCTTATTACAA+TGG - chr2_4:2459140-2459159 Msa0308260:intron 20.0%
!!! TTGTTTAATTTGAGGGTATT+TGG + chr2_4:2456988-2457007 None:intergenic 25.0%
!!! CAAAGAGTTGTTTAATTTGA+GGG + chr2_4:2456995-2457014 None:intergenic 25.0%
! TTAGTACATGTTTGATATCA+TGG - chr2_4:2457093-2457112 Msa0308260:three_prime_UTR 25.0%
!!! GTTTTTAGGAAAAATATTGG+TGG - chr2_4:2457228-2457247 Msa0308260:three_prime_UTR 25.0%
! AGTAAGTAACAACTTTGAAT+TGG - chr2_4:2457537-2457556 Msa0308260:CDS 25.0%
! AACAATTGAGAAAATTCCAA+TGG + chr2_4:2457675-2457694 None:intergenic 25.0%
!! GATTATATTAGTTTGGGATA+TGG - chr2_4:2458075-2458094 Msa0308260:CDS 25.0%
!! GTATTTTATGTGAATGTGTA+TGG - chr2_4:2458107-2458126 Msa0308260:CDS 25.0%
!! TTTTAGATCTGTATTAGATC+TGG - chr2_4:2458145-2458164 Msa0308260:CDS 25.0%
!!! TGTTACTGCTATTTTACTTA+AGG - chr2_4:2458227-2458246 Msa0308260:CDS 25.0%
! GACAAACTATCATAATATTC+TGG + chr2_4:2458900-2458919 None:intergenic 25.0%
! TATATTATGGACAAAACAGT+TGG - chr2_4:2458954-2458973 Msa0308260:intron 25.0%
! ACTTTCTTTGTTGAAATGTT+TGG + chr2_4:2459210-2459229 None:intergenic 25.0%
!! CCAAAGAGTTGTTTAATTTG+AGG + chr2_4:2456996-2457015 None:intergenic 30.0%
CCTCAAATTAAACAACTCTT+TGG - chr2_4:2456993-2457012 Msa0308260:three_prime_UTR 30.0%
AGTAACATTAGAGATCTAGA+TGG + chr2_4:2457059-2457078 None:intergenic 30.0%
CTAAAAACCATTGTTACACA+TGG + chr2_4:2457216-2457235 None:intergenic 30.0%
! CATGTGTAACAATGGTTTTT+AGG - chr2_4:2457214-2457233 Msa0308260:three_prime_UTR 30.0%
TAAAGATGTGAGATAACTTG+TGG - chr2_4:2457422-2457441 Msa0308260:exon 30.0%
! AAAGATGTGAGATAACTTGT+GGG - chr2_4:2457423-2457442 Msa0308260:exon 30.0%
TAAATCTGATGTGAGATTGT+AGG - chr2_4:2457504-2457523 Msa0308260:CDS 30.0%
AAATCTGATGTGAGATTGTA+GGG - chr2_4:2457505-2457524 Msa0308260:CDS 30.0%
! TTTTTCTCTCTCATGTTTGA+TGG - chr2_4:2457723-2457742 Msa0308260:CDS 30.0%
ATGTTGTATAAGATTTGCAG+TGG - chr2_4:2457829-2457848 Msa0308260:CDS 30.0%
ATGTGAATGTGTATGGTTTA+TGG - chr2_4:2458114-2458133 Msa0308260:CDS 30.0%
! CTATTTTACTTAAGGATGCA+GGG - chr2_4:2458235-2458254 Msa0308260:CDS 30.0%
!!! CTTCTAGTAGCCTATTTTTT+TGG - chr2_4:2458270-2458289 Msa0308260:intron 30.0%
ATAAACCATTGAGACATCAA+GGG + chr2_4:2458643-2458662 None:intergenic 30.0%
AATAAACCATTGAGACATCA+AGG + chr2_4:2458644-2458663 None:intergenic 30.0%
ATGTCTCAATGGTTTATTTC+TGG - chr2_4:2458646-2458665 Msa0308260:intron 30.0%
! TGTCTCAATGGTTTATTTCT+GGG - chr2_4:2458647-2458666 Msa0308260:intron 30.0%
TAAGAATATGTCTCTTGTTC+AGG + chr2_4:2458771-2458790 None:intergenic 30.0%
CAAGAGACATATTCTTACTA+CGG - chr2_4:2458774-2458793 Msa0308260:intron 30.0%
GTTTGTCACACTATAAATCA+TGG - chr2_4:2458913-2458932 Msa0308260:intron 30.0%
TTTCTCTTCATTCTGCTATA+AGG + chr2_4:2458981-2459000 None:intergenic 30.0%
! TCTATTCATTCAGTTTTGCT+TGG + chr2_4:2459005-2459024 None:intergenic 30.0%
! ACAATGGTGATGAATGTTAT+TGG - chr2_4:2459156-2459175 Msa0308260:intron 30.0%
AATGTTTGGACACATATTGA+TGG + chr2_4:2459196-2459215 None:intergenic 30.0%
AACTAGAGAATGTACATGAT+TGG - chr2_4:2459240-2459259 Msa0308260:intron 30.0%
GATTAGCAGATTACTAGTAA+TGG - chr2_4:2459303-2459322 Msa0308260:intron 30.0%
! TCAGTTTAAGGTTGAAGAAA+TGG - chr2_4:2459343-2459362 Msa0308260:intron 30.0%
CATTATTTGTCACTAGATAG+TGG - chr2_4:2459427-2459446 Msa0308260:CDS 30.0%
! AGATGAAGAAGGTGTAATGA+TGG - chr2_4:2456899-2456918 Msa0308260:three_prime_UTR 35.0%
!! TAATTTGAGGGTATTTGGAG+AGG + chr2_4:2456983-2457002 None:intergenic 35.0%
GATATGTTGCTGACATAGAT+AGG - chr2_4:2457307-2457326 Msa0308260:three_prime_UTR 35.0%
ACACCATTTGTGTGAAAAGT+TGG - chr2_4:2457472-2457491 Msa0308260:CDS 35.0%
! TCACCAACTTTTCACACAAA+TGG + chr2_4:2457478-2457497 None:intergenic 35.0%
! AAGTTGTTACTTACTTCTCG+TGG + chr2_4:2457532-2457551 None:intergenic 35.0%
AAAAGAGGAGATTGAGTTTG+TGG + chr2_4:2457707-2457726 None:intergenic 35.0%
CAAACATGAGAGAGAAAAAG+AGG + chr2_4:2457722-2457741 None:intergenic 35.0%
TCCAATGTTGACAATTCCAA+TGG - chr2_4:2457859-2457878 Msa0308260:CDS 35.0%
CAAGCTGAAATCAATTCTAG+AGG - chr2_4:2457971-2457990 Msa0308260:CDS 35.0%
ATCTACACTAGTGACAAGTT+TGG + chr2_4:2458058-2458077 None:intergenic 35.0%
!! GCTATTTTACTTAAGGATGC+AGG - chr2_4:2458234-2458253 Msa0308260:CDS 35.0%
GAGGAATGAGCCAAAAAAAT+AGG + chr2_4:2458283-2458302 None:intergenic 35.0%
! ATTTTCGCTAATCTCCCTAT+AGG - chr2_4:2458384-2458403 Msa0308260:intron 35.0%
!! TTTTGGAAGGTTACAGACAT+AGG + chr2_4:2458414-2458433 None:intergenic 35.0%
!! AACCTTCCAAAAGTATCACT+TGG - chr2_4:2458422-2458441 Msa0308260:intron 35.0%
ATGTTCTTGCGAAAACCATT+TGG - chr2_4:2458520-2458539 Msa0308260:intron 35.0%
TGTTCTTGCGAAAACCATTT+GGG - chr2_4:2458521-2458540 Msa0308260:intron 35.0%
TAGCTGTAAAAAGACCCAAA+TGG + chr2_4:2458538-2458557 None:intergenic 35.0%
GGTTTCGGTTTAAGAAACAA+CGG + chr2_4:2458579-2458598 None:intergenic 35.0%
TTGATGAATTCCCAAATACC+TGG + chr2_4:2458603-2458622 None:intergenic 35.0%
!! GTCTCAATGGTTTATTTCTG+GGG - chr2_4:2458648-2458667 Msa0308260:intron 35.0%
GAAAATCATGGTGTTCATTG+TGG + chr2_4:2458833-2458852 None:intergenic 35.0%
CAGGAATTCTTGGAAAATCA+TGG + chr2_4:2458845-2458864 None:intergenic 35.0%
! CATGATTTTCCAAGAATTCC+TGG - chr2_4:2458843-2458862 Msa0308260:intron 35.0%
! AGTTTGTTTCCAGGAATTCT+TGG + chr2_4:2458855-2458874 None:intergenic 35.0%
AAACAAACTTCACAAGGTGA+AGG - chr2_4:2458866-2458885 Msa0308260:intron 35.0%
ACACTATAAATCATGGAGTC+AGG - chr2_4:2458920-2458939 Msa0308260:intron 35.0%
!! AAACAAATGGCTTGCTATCT+GGG + chr2_4:2459125-2459144 None:intergenic 35.0%
!! TAAACAAATGGCTTGCTATC+TGG + chr2_4:2459126-2459145 None:intergenic 35.0%
! TAATGGACAGAACATGATCA+TGG - chr2_4:2459320-2459339 Msa0308260:intron 35.0%
ACATGATCATGGTCAGTTTA+AGG - chr2_4:2459331-2459350 Msa0308260:intron 35.0%
! TGATGGCTGGTGATTTCTTT+TGG - chr2_4:2456916-2456935 Msa0308260:three_prime_UTR 40.0%
CACATGTTTGGTATCACGAT+GGG + chr2_4:2457176-2457195 None:intergenic 40.0%
ATTGTTACACATGGCATTCG+GGG + chr2_4:2457207-2457226 None:intergenic 40.0%
CATTGTTACACATGGCATTC+GGG + chr2_4:2457208-2457227 None:intergenic 40.0%
CCATTGTTACACATGGCATT+CGG + chr2_4:2457209-2457228 None:intergenic 40.0%
!! TCAAGGTGTCAAAGGGTTTT+GGG + chr2_4:2457256-2457275 None:intergenic 40.0%
GGGGTCATCTCTAAATCTAA+TGG + chr2_4:2457568-2457587 None:intergenic 40.0%
ATAGAACCAATGACACACTG+GGG + chr2_4:2457587-2457606 None:intergenic 40.0%
CATAGAACCAATGACACACT+GGG + chr2_4:2457588-2457607 None:intergenic 40.0%
TCATAGAACCAATGACACAC+TGG + chr2_4:2457589-2457608 None:intergenic 40.0%
! AGGGAATTGATTCTCTCCAT+TGG - chr2_4:2457656-2457675 Msa0308260:CDS 40.0%
AATCCATTTCCGTGAGCTTA+AGG - chr2_4:2457795-2457814 Msa0308260:CDS 40.0%
TTACCTTAAGCTCACGGAAA+TGG + chr2_4:2457801-2457820 None:intergenic 40.0%
AGCATGTTACCTTAAGCTCA+CGG + chr2_4:2457807-2457826 None:intergenic 40.0%
GCCATTGGAATTGTCAACAT+TGG + chr2_4:2457863-2457882 None:intergenic 40.0%
AAGCTGCAAATCTTAGCCAT+TGG + chr2_4:2457878-2457897 None:intergenic 40.0%
! TTTTACTTAAGGATGCAGGG+TGG - chr2_4:2458238-2458257 Msa0308260:CDS 40.0%
! CTCCAAGTGATACTTTTGGA+AGG + chr2_4:2458427-2458446 None:intergenic 40.0%
TTTCTTAAACCGAAACCTCC+AGG - chr2_4:2458582-2458601 Msa0308260:intron 40.0%
ATGAATTCCCAAATACCTGG+AGG + chr2_4:2458600-2458619 None:intergenic 40.0%
!! CAATGGTTTATTTCTGGGGT+TGG - chr2_4:2458652-2458671 Msa0308260:intron 40.0%
GGCTAGTATGAGATAAGCTA+AGG + chr2_4:2458682-2458701 None:intergenic 40.0%
CTCATACTAGCCACATTTGT+TGG - chr2_4:2458690-2458709 Msa0308260:intron 40.0%
! ATACTAGCCACATTTGTTGG+TGG - chr2_4:2458693-2458712 Msa0308260:intron 40.0%
GACCTTTGAATCTTCTCACA+TGG - chr2_4:2458796-2458815 Msa0308260:intron 40.0%
TGCCATGTGAGAAGATTCAA+AGG + chr2_4:2458801-2458820 None:intergenic 40.0%
AATCTTCTCACATGGCATGT+AGG - chr2_4:2458804-2458823 Msa0308260:intron 40.0%
TCCTGGAAACAAACTTCACA+AGG - chr2_4:2458860-2458879 Msa0308260:intron 40.0%
! ACCTTGTGAAGTTTGTTTCC+AGG + chr2_4:2458864-2458883 None:intergenic 40.0%
GTTTGGACACATATTGATGG+AGG + chr2_4:2459193-2459212 None:intergenic 40.0%
ATCTGCTAATCTTTCCACTC+CGG + chr2_4:2459294-2459313 None:intergenic 40.0%
! GAAGAAGGTGTAATGATGGC+TGG - chr2_4:2456903-2456922 Msa0308260:three_prime_UTR 45.0%
ATTTGGAGAGGATCTGACGA+CGG + chr2_4:2456971-2456990 None:intergenic 45.0%
TTGAAGAATGCGAAGGGATC+AGG + chr2_4:2457020-2457039 None:intergenic 45.0%
TGATCCCTTCGCATTCTTCA+AGG - chr2_4:2457019-2457038 Msa0308260:three_prime_UTR 45.0%
CTCACCTTGAAGAATGCGAA+GGG + chr2_4:2457026-2457045 None:intergenic 45.0%
ACTCACCTTGAAGAATGCGA+AGG + chr2_4:2457027-2457046 None:intergenic 45.0%
CCACATGTTTGGTATCACGA+TGG + chr2_4:2457177-2457196 None:intergenic 45.0%
CCATCGTGATACCAAACATG+TGG - chr2_4:2457174-2457193 Msa0308260:three_prime_UTR 45.0%
CCGAATGCCATGTGTAACAA+TGG - chr2_4:2457206-2457225 Msa0308260:three_prime_UTR 45.0%
!! CAAGGTGTCAAAGGGTTTTG+GGG + chr2_4:2457255-2457274 None:intergenic 45.0%
!! CTCAAGGTGTCAAAGGGTTT+TGG + chr2_4:2457257-2457276 None:intergenic 45.0%
! CTCTCTCTCAAGGTGTCAAA+GGG + chr2_4:2457263-2457282 None:intergenic 45.0%
! TCTCTCTCTCAAGGTGTCAA+AGG + chr2_4:2457264-2457283 None:intergenic 45.0%
!! CGGAAATGGATTGTCAACGT+TGG + chr2_4:2457787-2457806 None:intergenic 45.0%
CCAAGAAGAGATTGTGGTTG+AGG + chr2_4:2458302-2458321 None:intergenic 45.0%
CCTCAACCACAATCTCTTCT+TGG - chr2_4:2458299-2458318 Msa0308260:intron 45.0%
GGATAGCCAAGAAGAGATTG+TGG + chr2_4:2458308-2458327 None:intergenic 45.0%
CAAGATTGTGACTGAGCTCA+TGG + chr2_4:2458329-2458348 None:intergenic 45.0%
TAGCGAAAATCCCAAGCCAA+CGG + chr2_4:2458374-2458393 None:intergenic 45.0%
CAGACATAGGTACGCCTATA+GGG + chr2_4:2458401-2458420 None:intergenic 45.0%
ACAGACATAGGTACGCCTAT+AGG + chr2_4:2458402-2458421 None:intergenic 45.0%
! GGTGCTCCAAGTGATACTTT+TGG + chr2_4:2458431-2458450 None:intergenic 45.0%
TATGGATATCCCCAGTCACA+TGG - chr2_4:2458479-2458498 Msa0308260:intron 45.0%
ACATGTGCTTCCATGTGACT+GGG + chr2_4:2458492-2458511 None:intergenic 45.0%
AACCGAAACCTCCAGGTATT+TGG - chr2_4:2458589-2458608 Msa0308260:intron 45.0%
ACCGAAACCTCCAGGTATTT+GGG - chr2_4:2458590-2458609 Msa0308260:intron 45.0%
TCCCAAATACCTGGAGGTTT+CGG + chr2_4:2458594-2458613 None:intergenic 45.0%
GATTGCCCTTGATGTCTCAA+TGG - chr2_4:2458635-2458654 Msa0308260:intron 45.0%
CATTCCTCCACCAACAAATG+TGG + chr2_4:2458703-2458722 None:intergenic 45.0%
GAAATGAAGTGACCAGCCAT+TGG + chr2_4:2458729-2458748 None:intergenic 45.0%
ACTACAGTCAACATGCGTTC+CGG - chr2_4:2459272-2459291 Msa0308260:intron 45.0%
GGAGGAGGAGAAGATGAAGA+AGG - chr2_4:2456888-2456907 Msa0308260:three_prime_UTR 50.0%
! GCGAGAAGCATGAGGTTCAT+CGG + chr2_4:2456949-2456968 None:intergenic 50.0%
GGGGTCAATCTCCACATGTT+TGG + chr2_4:2457188-2457207 None:intergenic 50.0%
ACACCTTGAGAGAGAGAGAG+AGG - chr2_4:2457267-2457286 Msa0308260:three_prime_UTR 50.0%
ATGCCTCTCTCTCTCTCTCA+AGG + chr2_4:2457273-2457292 None:intergenic 50.0%
AGATGACCCCAGTGTGTCAT+TGG - chr2_4:2457578-2457597 Msa0308260:CDS 50.0%
CAACTCCTGTCTACAACCGT+TGG - chr2_4:2458355-2458374 Msa0308260:intron 50.0%
!! CTGTCTACAACCGTTGGCTT+GGG - chr2_4:2458361-2458380 Msa0308260:intron 50.0%
CATGTGCTTCCATGTGACTG+GGG + chr2_4:2458491-2458510 None:intergenic 50.0%
GACATGTGCTTCCATGTGAC+TGG + chr2_4:2458493-2458512 None:intergenic 50.0%
! CTAGCCACATTTGTTGGTGG+AGG - chr2_4:2458696-2458715 Msa0308260:intron 50.0%
TGGTGGAGGAATGCATCCAA+TGG - chr2_4:2458710-2458729 Msa0308260:intron 50.0%
CCAAGCCAACGGTTGTAGAC+AGG + chr2_4:2458363-2458382 None:intergenic 55.0%
! CCTGTCTACAACCGTTGGCT+TGG - chr2_4:2458360-2458379 Msa0308260:intron 55.0%
! TTGGAGCACCATCGCTTCCA+AGG - chr2_4:2458441-2458460 Msa0308260:intron 55.0%
TATCAACGCCATCGACGCCT+TGG + chr2_4:2458461-2458480 None:intergenic 55.0%
!! AGGCGTCGATGGCGTTGATA+TGG - chr2_4:2458461-2458480 Msa0308260:intron 55.0%
GGAGGAATGCATCCAATGGC+TGG - chr2_4:2458714-2458733 Msa0308260:intron 55.0%
AGTCAACATGCGTTCCGGAG+TGG - chr2_4:2459277-2459296 Msa0308260:intron 55.0%
ATGGGTAAAGGCGGAGGAGG+AGG - chr2_4:2456870-2456889 Msa0308260:three_prime_UTR 60.0%
! TGACGACGGCGAGAAGCATG+AGG + chr2_4:2456957-2456976 None:intergenic 60.0%
CATCGACGCCTTGGAAGCGA+TGG + chr2_4:2458452-2458471 None:intergenic 60.0%
!! CATCGCTTCCAAGGCGTCGA+TGG - chr2_4:2458450-2458469 Msa0308260:intron 60.0%
GGTAAAGGCGGAGGAGGAGG+AGG - chr2_4:2456873-2456892 Msa0308260:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 2456870 2459590 2456870 ID=Msa0308260;Name=Msa0308260
chr2_4 mRNA 2456870 2459590 2456870 ID=Msa0308260-mRNA-1;Parent=Msa0308260;Name=Msa0308260-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|571|329
chr2_4 exon 2456870 2458259 2456870 ID=Msa0308260-mRNA-1:exon:605;Parent=Msa0308260-mRNA-1
chr2_4 exon 2459420 2459590 2459420 ID=Msa0308260-mRNA-1:exon:604;Parent=Msa0308260-mRNA-1
chr2_4 CDS 2459420 2459590 2459420 ID=Msa0308260-mRNA-1:cds;Parent=Msa0308260-mRNA-1
chr2_4 CDS 2457441 2458259 2457441 ID=Msa0308260-mRNA-1:cds;Parent=Msa0308260-mRNA-1
chr2_4 three_prime_UTR 2456870 2457440 2456870 ID=Msa0308260-mRNA-1:three_prime_utr;Parent=Msa0308260-mRNA-1
Gene Sequence

>Msa0308260

ATGGGTAAAGGCGGAGGAGGAGGAGGAGAAGATGAAGAAGGTGTAATGATGGCTGGTGATTTCTTTTGGTCATATACCGATGAACCTCATGCTTCTCGCCGTCGTCAGATCCTCTCCAAATACCCTCAAATTAAACAACTCTTTGGTCCTGATCCCTTCGCATTCTTCAAGATTAGTGTTGTTGTTCTGCTTCAACTTGTTACTGCTATTTTACTTAAGGATGCAGGGTGGCTGAAGATACTTCTAGTAGCCTATTTTTTTGGCTCATTCCTCAACCACAATCTCTTCTTGGCTATCCATGAGCTCAGTCACAATCTTGCTTTTTCAACTCCTGTCTACAACCGTTGGCTTGGGATTTTCGCTAATCTCCCTATAGGCGTACCTATGTCTGTAACCTTCCAAAAGTATCACTTGGAGCACCATCGCTTCCAAGGCGTCGATGGCGTTGATATGGATATCCCCAGTCACATGGAAGCACATGTCGTAACAAATGTTCTTGCGAAAACCATTTGGGTCTTTTTACAGCTATTCTTTTATGCATTTCGACCGTTGTTTCTTAAACCGAAACCTCCAGGTATTTGGGAATTCATCAACTTCTCTGTTCAGATTGCCCTTGATGTCTCAATGGTTTATTTCTGGGGTTGGAAATCCTTAGCTTATCTCATACTAGCCACATTTGTTGGTGGAGGAATGCATCCAATGGCTGGTCACTTCATTTCAGAACATTATGTATTCAATCCTGAACAAGAGACATATTCTTACTACGGACCTTTGAATCTTCTCACATGGCATGTAGGTTACCACAATGAACACCATGATTTTCCAAGAATTCCTGGAAACAAACTTCACAAGGTGAAGGAGATTGCACCAGAATATTATGATAGTTTGTCACACTATAAATCATGGAGTCAGGTTATTTACATGTATATTATGGACAAAACAGTTGGACCTTATAGCAGAATGAAGAGAAAGCCAAGCAAAACTGAATGA
Protein sequence

>Msa0308260

MGKGGGGGGEDEEGVMMAGDFFWSYTDEPHASRRRQILSKYPQIKQLFGPDPFAFFKISVVVLLQLVTAILLKDAGWLKILLVAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGVDMDIPSHMEAHVVTNVLAKTIWVFLQLFFYAFRPLFLKPKPPGIWEFINFSVQIALDVSMVYFWGWKSLAYLILATFVGGGMHPMAGHFISEHYVFNPEQETYSYYGPLNLLTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDSLSHYKSWSQVIYMYIMDKTVGPYSRMKRKPSKTE*