AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0234040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0234040 MtrunA17_Chr2g0281851 97.568 329 7 1 1 329 27 354 0.0 660
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0234040 AT4G04930.1 81.682 333 56 2 1 329 1 332 0.0 567

Find 102 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCTCAATGGTTTATTTC+TGG 0.042011 2_2:-6507905 Msa0234040:CDS
AACCGAAACCTCCAGGTATT+TGG 0.110935 2_2:-6507962 Msa0234040:CDS
TAAACAAATGGCTTGCTATC+TGG 0.171894 2_2:+6507427 None:intergenic
ACATGATCATGGTCAGTTTA+AGG 0.208576 2_2:-6507219 Msa0234040:three_prime_UTR
GGTTATTTACATGTATATTA+TGG 0.220265 2_2:-6507610 Msa0234040:CDS
TTGTTTAATTTGAGGGTATT+TGG 0.221781 2_2:+6509566 None:intergenic
TTTCTCTTCATTCTGCTATA+AGG 0.250507 2_2:+6507573 None:intergenic
CCTCAACCACAATCTCTTCT+TGG 0.268270 2_2:-6508252 Msa0234040:CDS
ACAATGGTGATGAATGTTAT+TGG 0.292521 2_2:-6507394 Msa0234040:three_prime_UTR
TGTCTCAATGGTTTATTTCT+GGG 0.295906 2_2:-6507904 Msa0234040:CDS
TGATGGCTGGTGATTTCTTT+TGG 0.300981 2_2:-6509635 Msa0234040:CDS
ACTTTCTTTGTTGAAATGTT+TGG 0.306805 2_2:+6507343 None:intergenic
AGGCGTCGATGGCGTTGATA+TGG 0.307309 2_2:-6508090 Msa0234040:CDS
ACCTTGTGAAGTTTGTTTCC+AGG 0.308162 2_2:+6507690 None:intergenic
TCCCAAATACCTGGAGGTTT+CGG 0.315484 2_2:+6507960 None:intergenic
TAAGAATATGTCTCTTGTTC+AGG 0.318858 2_2:+6507783 None:intergenic
ACCGAAACCTCCAGGTATTT+GGG 0.325860 2_2:-6507961 Msa0234040:CDS
CCTCAAATTAAACAACTCTT+TGG 0.348261 2_2:-6509558 Msa0234040:CDS
AGTTTGTTTCCAGGAATTCT+TGG 0.349785 2_2:+6507699 None:intergenic
AAACAAATGGCTTGCTATCT+GGG 0.353317 2_2:+6507428 None:intergenic
GGTAAAGGCGGAGGAGGAGG+AGG 0.367528 2_2:-6509678 Msa0234040:CDS
CTCATACTAGCCACATTTGT+TGG 0.367569 2_2:-6507861 Msa0234040:CDS
ATGTTCTTGCGAAAACCATT+TGG 0.369501 2_2:-6508031 Msa0234040:CDS
CAGGAATTCTTGGAAAATCA+TGG 0.376419 2_2:+6507709 None:intergenic
GGTGCTCCAAGTGATACTTT+TGG 0.379124 2_2:+6508123 None:intergenic
TGATCCCTTCGCATTCTTCA+AGG 0.379772 2_2:-6509532 Msa0234040:intron
GACAAACTATCATAATATTC+TGG 0.380490 2_2:+6507654 None:intergenic
AGTTTATAAAGAATATTCAT+AGG 0.384167 2_2:+6507505 None:intergenic
ATGGGTAAAGGCGGAGGAGG+AGG 0.384192 2_2:-6509681 Msa0234040:CDS
TTGAAGAATGCGAAGGGATC+AGG 0.397880 2_2:+6509534 None:intergenic
TGTTCTTGCGAAAACCATTT+GGG 0.401291 2_2:-6508030 Msa0234040:CDS
CAATGGTTTATTTCTGGGGT+TGG 0.403013 2_2:-6507899 Msa0234040:CDS
CTGTCTACAACCGTTGGCTT+GGG 0.405458 2_2:-6508190 Msa0234040:CDS
TCAGTTTAAGGTTGAAGAAA+TGG 0.411609 2_2:-6507207 Msa0234040:three_prime_UTR
CAAAGAGTTGTTTAATTTGA+GGG 0.413350 2_2:+6509559 None:intergenic
CCAAAGAGTTGTTTAATTTG+AGG 0.414984 2_2:+6509558 None:intergenic
CAGACATAGGTACGCCTATA+GGG 0.434208 2_2:+6508153 None:intergenic
AATGTTTGGACACATATTGA+TGG 0.435853 2_2:+6507357 None:intergenic
CATTATTTGTCACTAGATAG+TGG 0.442233 2_2:-6507123 Msa0234040:three_prime_UTR
AAAATGGGTAAAGGCGGAGG+AGG 0.447267 2_2:-6509684 Msa0234040:CDS
CCTGTCTACAACCGTTGGCT+TGG 0.454505 2_2:-6508191 Msa0234040:CDS
CCAAGAAGAGATTGTGGTTG+AGG 0.459624 2_2:+6508252 None:intergenic
TATCAACGCCATCGACGCCT+TGG 0.464202 2_2:+6508093 None:intergenic
ACTACAGTCAACATGCGTTC+CGG 0.466049 2_2:-6507278 Msa0234040:three_prime_UTR
ACAGACATAGGTACGCCTAT+AGG 0.470286 2_2:+6508152 None:intergenic
GATTGCCCTTGATGTCTCAA+TGG 0.470604 2_2:-6507916 Msa0234040:CDS
AATAAACCATTGAGACATCA+AGG 0.477537 2_2:+6507910 None:intergenic
GTTTATAAAGAATATTCATA+GGG 0.479471 2_2:+6507506 None:intergenic
CATCGCTTCCAAGGCGTCGA+TGG 0.485169 2_2:-6508101 Msa0234040:CDS
GTAATAAGCTAATAAACAAA+TGG 0.485651 2_2:+6507415 None:intergenic
CAAGATTGTGACTGAGCTCA+TGG 0.487021 2_2:+6508225 None:intergenic
GACATGTGCTTCCATGTGAC+TGG 0.492679 2_2:+6508061 None:intergenic
ACACTATAAATCATGGAGTC+AGG 0.494471 2_2:-6507631 Msa0234040:CDS
TTTCTTAAACCGAAACCTCC+AGG 0.504284 2_2:-6507969 Msa0234040:CDS
GTCTCAATGGTTTATTTCTG+GGG 0.504698 2_2:-6507903 Msa0234040:CDS
CCAAGCCAACGGTTGTAGAC+AGG 0.507037 2_2:+6508191 None:intergenic
GATTAGCAGATTACTAGTAA+TGG 0.512849 2_2:-6507247 Msa0234040:three_prime_UTR
GTTTGTCACACTATAAATCA+TGG 0.516695 2_2:-6507638 Msa0234040:CDS
GGAGGAGGAGAAGATGAAGA+AGG 0.517452 2_2:-6509663 Msa0234040:CDS
CAACTCCTGTCTACAACCGT+TGG 0.519760 2_2:-6508196 Msa0234040:CDS
AATCTTCTCACATGGCATGT+AGG 0.525317 2_2:-6507747 Msa0234040:CDS
TTGGAGCACCATCGCTTCCA+AGG 0.530014 2_2:-6508110 Msa0234040:CDS
AACCTTCCAAAAGTATCACT+TGG 0.535024 2_2:-6508129 Msa0234040:CDS
ATACTAGCCACATTTGTTGG+TGG 0.545798 2_2:-6507858 Msa0234040:CDS
CTAGCCACATTTGTTGGTGG+AGG 0.551745 2_2:-6507855 Msa0234040:CDS
AGATGAAGAAGGTGTAATGA+TGG 0.551961 2_2:-6509652 Msa0234040:CDS
AACTAGAGAATGTACATGAT+TGG 0.552599 2_2:-6507310 Msa0234040:three_prime_UTR
TATATTATGGACAAAACAGT+TGG 0.553526 2_2:-6507597 Msa0234040:CDS
TGTTTATTAGCTTATTACAA+TGG 0.556839 2_2:-6507410 Msa0234040:three_prime_UTR
CAAGAGACATATTCTTACTA+CGG 0.561519 2_2:-6507777 Msa0234040:CDS
GGCTAGTATGAGATAAGCTA+AGG 0.565655 2_2:+6507872 None:intergenic
TAATTTGAGGGTATTTGGAG+AGG 0.567285 2_2:+6509571 None:intergenic
TTGATGAATTCCCAAATACC+TGG 0.573711 2_2:+6507951 None:intergenic
GAAATGAAGTGACCAGCCAT+TGG 0.573733 2_2:+6507825 None:intergenic
GGAGGAATGCATCCAATGGC+TGG 0.575417 2_2:-6507837 Msa0234040:CDS
TAATGGACAGAACATGATCA+TGG 0.577168 2_2:-6507230 Msa0234040:three_prime_UTR
GAAGAAGGTGTAATGATGGC+TGG 0.581573 2_2:-6509648 Msa0234040:CDS
CATCGACGCCTTGGAAGCGA+TGG 0.586947 2_2:+6508102 None:intergenic
ATCTGCTAATCTTTCCACTC+CGG 0.594440 2_2:+6507259 None:intergenic
GACCTTTGAATCTTCTCACA+TGG 0.598138 2_2:-6507755 Msa0234040:CDS
GCGAGAAGCATGAGGTTCAT+CGG 0.598370 2_2:+6509605 None:intergenic
TGCCATGTGAGAAGATTCAA+AGG 0.605214 2_2:+6507753 None:intergenic
CATTCCTCCACCAACAAATG+TGG 0.605926 2_2:+6507851 None:intergenic
ACATGTGCTTCCATGTGACT+GGG 0.609728 2_2:+6508062 None:intergenic
GTTTGGACACATATTGATGG+AGG 0.617544 2_2:+6507360 None:intergenic
TATGGATATCCCCAGTCACA+TGG 0.618891 2_2:-6508072 Msa0234040:CDS
CTCACCTTGAAGAATGCGAA+GGG 0.618939 2_2:+6509528 None:intergenic
TCCTGGAAACAAACTTCACA+AGG 0.620833 2_2:-6507691 Msa0234040:CDS
AGTCAACATGCGTTCCGGAG+TGG 0.622695 2_2:-6507273 Msa0234040:three_prime_UTR
GAAAATCATGGTGTTCATTG+TGG 0.623404 2_2:+6507721 None:intergenic
ACTCACCTTGAAGAATGCGA+AGG 0.629782 2_2:+6509527 None:intergenic
GGATAGCCAAGAAGAGATTG+TGG 0.634306 2_2:+6508246 None:intergenic
ATAAACCATTGAGACATCAA+GGG 0.649348 2_2:+6507911 None:intergenic
AAACAAACTTCACAAGGTGA+AGG 0.651727 2_2:-6507685 Msa0234040:CDS
TTTATAAAGAATATTCATAG+GGG 0.654681 2_2:+6507507 None:intergenic
TAGCGAAAATCCCAAGCCAA+CGG 0.655542 2_2:+6508180 None:intergenic
TGACGACGGCGAGAAGCATG+AGG 0.663294 2_2:+6509597 None:intergenic
ATGAATTCCCAAATACCTGG+AGG 0.666515 2_2:+6507954 None:intergenic
TGGTGGAGGAATGCATCCAA+TGG 0.675962 2_2:-6507841 Msa0234040:CDS
GGTTTCGGTTTAAGAAACAA+CGG 0.699596 2_2:+6507975 None:intergenic
CATGTGCTTCCATGTGACTG+GGG 0.710334 2_2:+6508063 None:intergenic
ATTTGGAGAGGATCTGACGA+CGG 0.745086 2_2:+6509583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAATGTATTAAAAAGA+TGG - chr2_2:6509616-6509635 Msa0234040:CDS 10.0%
!!! AAATTGATTTTGATATGTTT+GGG + chr2_2:6508133-6508152 None:intergenic 15.0%
!!! AAAATTGATTTTGATATGTT+TGG + chr2_2:6508134-6508153 None:intergenic 15.0%
!! TTTATAAAGAATATTCATAG+GGG + chr2_2:6509159-6509178 None:intergenic 15.0%
!! GTTTATAAAGAATATTCATA+GGG + chr2_2:6509160-6509179 None:intergenic 15.0%
!! AGTTTATAAAGAATATTCAT+AGG + chr2_2:6509161-6509180 None:intergenic 15.0%
!!! TCTTGAATTTCATTTTAATT+AGG - chr2_2:6509572-6509591 Msa0234040:CDS 15.0%
!! TATCAAACATGTACTAAAAA+CGG + chr2_2:6507202-6507221 None:intergenic 20.0%
!! AGAATCATATATTAGAATCA+CGG - chr2_2:6507238-6507257 Msa0234040:three_prime_UTR 20.0%
!!! ATGGTTTTTAGGAAAAATAT+TGG - chr2_2:6507337-6507356 Msa0234040:three_prime_UTR 20.0%
!!! TTTTTAGGAAAAATATTGGT+GGG - chr2_2:6507341-6507360 Msa0234040:three_prime_UTR 20.0%
!!! TTTTAGTTTAAAGTGAGTTT+AGG - chr2_2:6507748-6507767 Msa0234040:CDS 20.0%
!!! TTTAGTTTAAAGTGAGTTTA+GGG - chr2_2:6507749-6507768 Msa0234040:CDS 20.0%
!! AAGTATGTTGAACAATAATT+TGG - chr2_2:6508022-6508041 Msa0234040:CDS 20.0%
!! AGTATGTTGAACAATAATTT+GGG - chr2_2:6508023-6508042 Msa0234040:CDS 20.0%
!! AATAATTTGGGTGTAAAATT+TGG - chr2_2:6508035-6508054 Msa0234040:CDS 20.0%
!! TAGTGTAGATTATATTAGTT+TGG - chr2_2:6508180-6508199 Msa0234040:CDS 20.0%
!!! AGTGTAGATTATATTAGTTT+GGG - chr2_2:6508181-6508200 Msa0234040:CDS 20.0%
!! GGTTATTTACATGTATATTA+TGG - chr2_2:6509053-6509072 Msa0234040:intron 20.0%
!! GTAATAAGCTAATAAACAAA+TGG + chr2_2:6509251-6509270 None:intergenic 20.0%
!!! TGTTTATTAGCTTATTACAA+TGG - chr2_2:6509253-6509272 Msa0234040:intron 20.0%
!!! TTGTTTAATTTGAGGGTATT+TGG + chr2_2:6507100-6507119 None:intergenic 25.0%
!!! CAAAGAGTTGTTTAATTTGA+GGG + chr2_2:6507107-6507126 None:intergenic 25.0%
! TTAGTACATGTTTGATATCA+TGG - chr2_2:6507205-6507224 Msa0234040:three_prime_UTR 25.0%
!!! GTTTTTAGGAAAAATATTGG+TGG - chr2_2:6507340-6507359 Msa0234040:three_prime_UTR 25.0%
! AGTAAGTAACAACTTTGAAT+TGG - chr2_2:6507649-6507668 Msa0234040:CDS 25.0%
! AACAATTGAGAAAATTCCAA+TGG + chr2_2:6507787-6507806 None:intergenic 25.0%
!! GATTATATTAGTTTGGGATA+TGG - chr2_2:6508187-6508206 Msa0234040:CDS 25.0%
!! GTATTTTATGTGAATGTGTA+TGG - chr2_2:6508219-6508238 Msa0234040:CDS 25.0%
!! TTTTAGATCTGTATTAGATC+TGG - chr2_2:6508257-6508276 Msa0234040:CDS 25.0%
!!! TGTTACTGCTATTTTACTTA+AGG - chr2_2:6508339-6508358 Msa0234040:CDS 25.0%
! GACAAACTATCATAATATTC+TGG + chr2_2:6509012-6509031 None:intergenic 25.0%
! TATATTATGGACAAAACAGT+TGG - chr2_2:6509066-6509085 Msa0234040:intron 25.0%
! ACTTTCTTTGTTGAAATGTT+TGG + chr2_2:6509323-6509342 None:intergenic 25.0%
!! CCAAAGAGTTGTTTAATTTG+AGG + chr2_2:6507108-6507127 None:intergenic 30.0%
CCTCAAATTAAACAACTCTT+TGG - chr2_2:6507105-6507124 Msa0234040:three_prime_UTR 30.0%
AGTAACATTAGAGATCTAGA+TGG + chr2_2:6507171-6507190 None:intergenic 30.0%
CTAAAAACCATTGTTACACA+TGG + chr2_2:6507328-6507347 None:intergenic 30.0%
! CATGTGTAACAATGGTTTTT+AGG - chr2_2:6507326-6507345 Msa0234040:three_prime_UTR 30.0%
TAAAGATGTGAGATAACTTG+TGG - chr2_2:6507534-6507553 Msa0234040:three_prime_UTR 30.0%
! AAAGATGTGAGATAACTTGT+GGG - chr2_2:6507535-6507554 Msa0234040:exon 30.0%
TAAATCTGATGTGAGATTGT+AGG - chr2_2:6507616-6507635 Msa0234040:CDS 30.0%
AAATCTGATGTGAGATTGTA+GGG - chr2_2:6507617-6507636 Msa0234040:CDS 30.0%
! TTTTTCTCTCTCATGTTTGA+TGG - chr2_2:6507835-6507854 Msa0234040:CDS 30.0%
ATGTTGTATAAGATTTGCAG+TGG - chr2_2:6507941-6507960 Msa0234040:CDS 30.0%
ATGTGAATGTGTATGGTTTA+TGG - chr2_2:6508226-6508245 Msa0234040:CDS 30.0%
! CTATTTTACTTAAGGATGCA+GGG - chr2_2:6508347-6508366 Msa0234040:CDS 30.0%
!!! CTTCTAGTAGCCTATTTTTT+TGG - chr2_2:6508382-6508401 Msa0234040:intron 30.0%
ATAAACCATTGAGACATCAA+GGG + chr2_2:6508755-6508774 None:intergenic 30.0%
AATAAACCATTGAGACATCA+AGG + chr2_2:6508756-6508775 None:intergenic 30.0%
ATGTCTCAATGGTTTATTTC+TGG - chr2_2:6508758-6508777 Msa0234040:intron 30.0%
! TGTCTCAATGGTTTATTTCT+GGG - chr2_2:6508759-6508778 Msa0234040:intron 30.0%
TAAGAATATGTCTCTTGTTC+AGG + chr2_2:6508883-6508902 None:intergenic 30.0%
CAAGAGACATATTCTTACTA+CGG - chr2_2:6508886-6508905 Msa0234040:intron 30.0%
GTTTGTCACACTATAAATCA+TGG - chr2_2:6509025-6509044 Msa0234040:intron 30.0%
TTTCTCTTCATTCTGCTATA+AGG + chr2_2:6509093-6509112 None:intergenic 30.0%
! TCTATTCATTCAGTTTTGCT+TGG + chr2_2:6509117-6509136 None:intergenic 30.0%
! ACAATGGTGATGAATGTTAT+TGG - chr2_2:6509269-6509288 Msa0234040:intron 30.0%
AATGTTTGGACACATATTGA+TGG + chr2_2:6509309-6509328 None:intergenic 30.0%
AACTAGAGAATGTACATGAT+TGG - chr2_2:6509353-6509372 Msa0234040:intron 30.0%
GATTAGCAGATTACTAGTAA+TGG - chr2_2:6509416-6509435 Msa0234040:intron 30.0%
! TCAGTTTAAGGTTGAAGAAA+TGG - chr2_2:6509456-6509475 Msa0234040:intron 30.0%
CATTATTTGTCACTAGATAG+TGG - chr2_2:6509540-6509559 Msa0234040:CDS 30.0%
! AGATGAAGAAGGTGTAATGA+TGG - chr2_2:6507011-6507030 Msa0234040:three_prime_UTR 35.0%
!! TAATTTGAGGGTATTTGGAG+AGG + chr2_2:6507095-6507114 None:intergenic 35.0%
GATATGTTGCTGACATAGAT+AGG - chr2_2:6507419-6507438 Msa0234040:three_prime_UTR 35.0%
ACACCATTTGTGTGAAAAGT+TGG - chr2_2:6507584-6507603 Msa0234040:CDS 35.0%
! TCACCAACTTTTCACACAAA+TGG + chr2_2:6507590-6507609 None:intergenic 35.0%
! AAGTTGTTACTTACTTCTCG+TGG + chr2_2:6507644-6507663 None:intergenic 35.0%
AAAAGAGGAGATTGAGTTTG+TGG + chr2_2:6507819-6507838 None:intergenic 35.0%
CAAACATGAGAGAGAAAAAG+AGG + chr2_2:6507834-6507853 None:intergenic 35.0%
TCCAATGTTGACAATTCCAA+TGG - chr2_2:6507971-6507990 Msa0234040:CDS 35.0%
CAAGCTGAAATCAATTCTAG+AGG - chr2_2:6508083-6508102 Msa0234040:CDS 35.0%
ATCTACACTAGTGACAAGTT+TGG + chr2_2:6508170-6508189 None:intergenic 35.0%
!! GCTATTTTACTTAAGGATGC+AGG - chr2_2:6508346-6508365 Msa0234040:CDS 35.0%
GAGGAATGAGCCAAAAAAAT+AGG + chr2_2:6508395-6508414 None:intergenic 35.0%
! ATTTTCGCTAATCTCCCTAT+AGG - chr2_2:6508496-6508515 Msa0234040:intron 35.0%
!! TTTTGGAAGGTTACAGACAT+AGG + chr2_2:6508526-6508545 None:intergenic 35.0%
!! AACCTTCCAAAAGTATCACT+TGG - chr2_2:6508534-6508553 Msa0234040:intron 35.0%
ATGTTCTTGCGAAAACCATT+TGG - chr2_2:6508632-6508651 Msa0234040:intron 35.0%
TGTTCTTGCGAAAACCATTT+GGG - chr2_2:6508633-6508652 Msa0234040:intron 35.0%
TAGCTGTAAAAAGACCCAAA+TGG + chr2_2:6508650-6508669 None:intergenic 35.0%
GGTTTCGGTTTAAGAAACAA+CGG + chr2_2:6508691-6508710 None:intergenic 35.0%
TTGATGAATTCCCAAATACC+TGG + chr2_2:6508715-6508734 None:intergenic 35.0%
!! GTCTCAATGGTTTATTTCTG+GGG - chr2_2:6508760-6508779 Msa0234040:intron 35.0%
GAAAATCATGGTGTTCATTG+TGG + chr2_2:6508945-6508964 None:intergenic 35.0%
CAGGAATTCTTGGAAAATCA+TGG + chr2_2:6508957-6508976 None:intergenic 35.0%
! CATGATTTTCCAAGAATTCC+TGG - chr2_2:6508955-6508974 Msa0234040:intron 35.0%
! AGTTTGTTTCCAGGAATTCT+TGG + chr2_2:6508967-6508986 None:intergenic 35.0%
AAACAAACTTCACAAGGTGA+AGG - chr2_2:6508978-6508997 Msa0234040:intron 35.0%
ACACTATAAATCATGGAGTC+AGG - chr2_2:6509032-6509051 Msa0234040:intron 35.0%
!! AAACAAATGGCTTGCTATCT+GGG + chr2_2:6509238-6509257 None:intergenic 35.0%
!! TAAACAAATGGCTTGCTATC+TGG + chr2_2:6509239-6509258 None:intergenic 35.0%
! TAATGGACAGAACATGATCA+TGG - chr2_2:6509433-6509452 Msa0234040:intron 35.0%
ACATGATCATGGTCAGTTTA+AGG - chr2_2:6509444-6509463 Msa0234040:intron 35.0%
! TGATGGCTGGTGATTTCTTT+TGG - chr2_2:6507028-6507047 Msa0234040:three_prime_UTR 40.0%
ATTGTTACACATGGCATTCG+GGG + chr2_2:6507319-6507338 None:intergenic 40.0%
CATTGTTACACATGGCATTC+GGG + chr2_2:6507320-6507339 None:intergenic 40.0%
CCATTGTTACACATGGCATT+CGG + chr2_2:6507321-6507340 None:intergenic 40.0%
!! TCAAGGTGTCAAAGGGTTTT+GGG + chr2_2:6507368-6507387 None:intergenic 40.0%
GGGGTCATCTCTAAATCTAA+TGG + chr2_2:6507680-6507699 None:intergenic 40.0%
ATAGAACCAATGACACACTG+GGG + chr2_2:6507699-6507718 None:intergenic 40.0%
CATAGAACCAATGACACACT+GGG + chr2_2:6507700-6507719 None:intergenic 40.0%
TCATAGAACCAATGACACAC+TGG + chr2_2:6507701-6507720 None:intergenic 40.0%
! AGGGAATTGATTCTCTCCAT+TGG - chr2_2:6507768-6507787 Msa0234040:CDS 40.0%
AATCCATTTCCGTGAGCTTA+AGG - chr2_2:6507907-6507926 Msa0234040:CDS 40.0%
TTACCTTAAGCTCACGGAAA+TGG + chr2_2:6507913-6507932 None:intergenic 40.0%
AGCATGTTACCTTAAGCTCA+CGG + chr2_2:6507919-6507938 None:intergenic 40.0%
GCCATTGGAATTGTCAACAT+TGG + chr2_2:6507975-6507994 None:intergenic 40.0%
AAGCTGCAAATCTTAGCCAT+TGG + chr2_2:6507990-6508009 None:intergenic 40.0%
! TTTTACTTAAGGATGCAGGG+TGG - chr2_2:6508350-6508369 Msa0234040:CDS 40.0%
! CTCCAAGTGATACTTTTGGA+AGG + chr2_2:6508539-6508558 None:intergenic 40.0%
TTTCTTAAACCGAAACCTCC+AGG - chr2_2:6508694-6508713 Msa0234040:intron 40.0%
ATGAATTCCCAAATACCTGG+AGG + chr2_2:6508712-6508731 None:intergenic 40.0%
!! CAATGGTTTATTTCTGGGGT+TGG - chr2_2:6508764-6508783 Msa0234040:intron 40.0%
GGCTAGTATGAGATAAGCTA+AGG + chr2_2:6508794-6508813 None:intergenic 40.0%
CTCATACTAGCCACATTTGT+TGG - chr2_2:6508802-6508821 Msa0234040:intron 40.0%
! ATACTAGCCACATTTGTTGG+TGG - chr2_2:6508805-6508824 Msa0234040:intron 40.0%
GACCTTTGAATCTTCTCACA+TGG - chr2_2:6508908-6508927 Msa0234040:intron 40.0%
TGCCATGTGAGAAGATTCAA+AGG + chr2_2:6508913-6508932 None:intergenic 40.0%
AATCTTCTCACATGGCATGT+AGG - chr2_2:6508916-6508935 Msa0234040:intron 40.0%
TCCTGGAAACAAACTTCACA+AGG - chr2_2:6508972-6508991 Msa0234040:intron 40.0%
! ACCTTGTGAAGTTTGTTTCC+AGG + chr2_2:6508976-6508995 None:intergenic 40.0%
GTTTGGACACATATTGATGG+AGG + chr2_2:6509306-6509325 None:intergenic 40.0%
ATCTGCTAATCTTTCCACTC+CGG + chr2_2:6509407-6509426 None:intergenic 40.0%
! GAAGAAGGTGTAATGATGGC+TGG - chr2_2:6507015-6507034 Msa0234040:three_prime_UTR 45.0%
ATTTGGAGAGGATCTGACGA+CGG + chr2_2:6507083-6507102 None:intergenic 45.0%
TTGAAGAATGCGAAGGGATC+AGG + chr2_2:6507132-6507151 None:intergenic 45.0%
TGATCCCTTCGCATTCTTCA+AGG - chr2_2:6507131-6507150 Msa0234040:three_prime_UTR 45.0%
CTCACCTTGAAGAATGCGAA+GGG + chr2_2:6507138-6507157 None:intergenic 45.0%
ACTCACCTTGAAGAATGCGA+AGG + chr2_2:6507139-6507158 None:intergenic 45.0%
CACATGTTTGGTACCACGAT+GGG + chr2_2:6507288-6507307 None:intergenic 45.0%
CCGAATGCCATGTGTAACAA+TGG - chr2_2:6507318-6507337 Msa0234040:three_prime_UTR 45.0%
!! CAAGGTGTCAAAGGGTTTTG+GGG + chr2_2:6507367-6507386 None:intergenic 45.0%
!! CTCAAGGTGTCAAAGGGTTT+TGG + chr2_2:6507369-6507388 None:intergenic 45.0%
! CTCTCTCTCAAGGTGTCAAA+GGG + chr2_2:6507375-6507394 None:intergenic 45.0%
! TCTCTCTCTCAAGGTGTCAA+AGG + chr2_2:6507376-6507395 None:intergenic 45.0%
!! CGGAAATGGATTGTCAACGT+TGG + chr2_2:6507899-6507918 None:intergenic 45.0%
CCAAGAAGAGATTGTGGTTG+AGG + chr2_2:6508414-6508433 None:intergenic 45.0%
CCTCAACCACAATCTCTTCT+TGG - chr2_2:6508411-6508430 Msa0234040:intron 45.0%
GGATAGCCAAGAAGAGATTG+TGG + chr2_2:6508420-6508439 None:intergenic 45.0%
CAAGATTGTGACTGAGCTCA+TGG + chr2_2:6508441-6508460 None:intergenic 45.0%
TAGCGAAAATCCCAAGCCAA+CGG + chr2_2:6508486-6508505 None:intergenic 45.0%
CAGACATAGGTACGCCTATA+GGG + chr2_2:6508513-6508532 None:intergenic 45.0%
ACAGACATAGGTACGCCTAT+AGG + chr2_2:6508514-6508533 None:intergenic 45.0%
! GGTGCTCCAAGTGATACTTT+TGG + chr2_2:6508543-6508562 None:intergenic 45.0%
TATGGATATCCCCAGTCACA+TGG - chr2_2:6508591-6508610 Msa0234040:intron 45.0%
ACATGTGCTTCCATGTGACT+GGG + chr2_2:6508604-6508623 None:intergenic 45.0%
AACCGAAACCTCCAGGTATT+TGG - chr2_2:6508701-6508720 Msa0234040:intron 45.0%
ACCGAAACCTCCAGGTATTT+GGG - chr2_2:6508702-6508721 Msa0234040:intron 45.0%
TCCCAAATACCTGGAGGTTT+CGG + chr2_2:6508706-6508725 None:intergenic 45.0%
GATTGCCCTTGATGTCTCAA+TGG - chr2_2:6508747-6508766 Msa0234040:intron 45.0%
CATTCCTCCACCAACAAATG+TGG + chr2_2:6508815-6508834 None:intergenic 45.0%
GAAATGAAGTGACCAGCCAT+TGG + chr2_2:6508841-6508860 None:intergenic 45.0%
ACTACAGTCAACATGCGTTC+CGG - chr2_2:6509385-6509404 Msa0234040:intron 45.0%
GGAGGAGGAGAAGATGAAGA+AGG - chr2_2:6507000-6507019 Msa0234040:three_prime_UTR 50.0%
! GCGAGAAGCATGAGGTTCAT+CGG + chr2_2:6507061-6507080 None:intergenic 50.0%
TTGCAAATCACAGCCCATCG+TGG - chr2_2:6507272-6507291 Msa0234040:three_prime_UTR 50.0%
CCACATGTTTGGTACCACGA+TGG + chr2_2:6507289-6507308 None:intergenic 50.0%
CCATCGTGGTACCAAACATG+TGG - chr2_2:6507286-6507305 Msa0234040:three_prime_UTR 50.0%
GGGGTCAATCTCCACATGTT+TGG + chr2_2:6507300-6507319 None:intergenic 50.0%
ACACCTTGAGAGAGAGAGAG+AGG - chr2_2:6507379-6507398 Msa0234040:three_prime_UTR 50.0%
ATGCCTCTCTCTCTCTCTCA+AGG + chr2_2:6507385-6507404 None:intergenic 50.0%
AGATGACCCCAGTGTGTCAT+TGG - chr2_2:6507690-6507709 Msa0234040:CDS 50.0%
CAACTCCTGTCTACAACCGT+TGG - chr2_2:6508467-6508486 Msa0234040:intron 50.0%
!! CTGTCTACAACCGTTGGCTT+GGG - chr2_2:6508473-6508492 Msa0234040:intron 50.0%
CATGTGCTTCCATGTGACTG+GGG + chr2_2:6508603-6508622 None:intergenic 50.0%
GACATGTGCTTCCATGTGAC+TGG + chr2_2:6508605-6508624 None:intergenic 50.0%
! CTAGCCACATTTGTTGGTGG+AGG - chr2_2:6508808-6508827 Msa0234040:intron 50.0%
TGGTGGAGGAATGCATCCAA+TGG - chr2_2:6508822-6508841 Msa0234040:intron 50.0%
CCAAGCCAACGGTTGTAGAC+AGG + chr2_2:6508475-6508494 None:intergenic 55.0%
! CCTGTCTACAACCGTTGGCT+TGG - chr2_2:6508472-6508491 Msa0234040:intron 55.0%
! TTGGAGCACCATCGCTTCCA+AGG - chr2_2:6508553-6508572 Msa0234040:intron 55.0%
TATCAACGCCATCGACGCCT+TGG + chr2_2:6508573-6508592 None:intergenic 55.0%
!! AGGCGTCGATGGCGTTGATA+TGG - chr2_2:6508573-6508592 Msa0234040:intron 55.0%
GGAGGAATGCATCCAATGGC+TGG - chr2_2:6508826-6508845 Msa0234040:intron 55.0%
AGTCAACATGCGTTCCGGAG+TGG - chr2_2:6509390-6509409 Msa0234040:intron 55.0%
ATGGGTAAAGGCGGAGGAGG+AGG - chr2_2:6506982-6507001 Msa0234040:three_prime_UTR 60.0%
! TGACGACGGCGAGAAGCATG+AGG + chr2_2:6507069-6507088 None:intergenic 60.0%
CATCGACGCCTTGGAAGCGA+TGG + chr2_2:6508564-6508583 None:intergenic 60.0%
!! CATCGCTTCCAAGGCGTCGA+TGG - chr2_2:6508562-6508581 Msa0234040:intron 60.0%
GGTAAAGGCGGAGGAGGAGG+AGG - chr2_2:6506985-6507004 Msa0234040:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 6506982 6509703 6506982 ID=Msa0234040;Name=Msa0234040
chr2_2 mRNA 6506982 6509703 6506982 ID=Msa0234040-mRNA-1;Parent=Msa0234040;Name=Msa0234040-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|572|329
chr2_2 exon 6506982 6508372 6506982 ID=Msa0234040-mRNA-1:exon:2909;Parent=Msa0234040-mRNA-1
chr2_2 exon 6509533 6509703 6509533 ID=Msa0234040-mRNA-1:exon:2908;Parent=Msa0234040-mRNA-1
chr2_2 CDS 6509533 6509703 6509533 ID=Msa0234040-mRNA-1:cds;Parent=Msa0234040-mRNA-1
chr2_2 CDS 6507554 6508372 6507554 ID=Msa0234040-mRNA-1:cds;Parent=Msa0234040-mRNA-1
chr2_2 three_prime_UTR 6506982 6507553 6506982 ID=Msa0234040-mRNA-1:three_prime_utr;Parent=Msa0234040-mRNA-1
Gene Sequence

>Msa0234040

ATGGGTAAAGGCGGAGGAGGAGGAGGAGAAGATGAAGAAGGTGTAATGATGGCTGGTGATTTCTTTTGGTCATATACCGATGAACCTCATGCTTCTCGCCGTCGTCAGATCCTCTCCAAATACCCTCAAATTAAACAACTCTTTGGTCCTGATCCCTTCGCATTCTTCAAGATTAGTGTTGTTGTTCTGCTTCAACTTGTTACTGCTATTTTACTTAAGGATGCAGGGTGGCTGAAGATACTTCTAGTAGCCTATTTTTTTGGCTCATTCCTCAACCACAATCTCTTCTTGGCTATCCATGAGCTCAGTCACAATCTTGCTTTTTCAACTCCTGTCTACAACCGTTGGCTTGGGATTTTCGCTAATCTCCCTATAGGCGTACCTATGTCTGTAACCTTCCAAAAGTATCACTTGGAGCACCATCGCTTCCAAGGCGTCGATGGCGTTGATATGGATATCCCCAGTCACATGGAAGCACATGTCGTAACAAATGTTCTTGCGAAAACCATTTGGGTCTTTTTACAGCTATTCTTTTATGCATTTCGACCGTTGTTTCTTAAACCGAAACCTCCAGGTATTTGGGAATTCATCAACTTCTCTGTTCAGATTGCCCTTGATGTCTCAATGGTTTATTTCTGGGGTTGGAAATCCTTAGCTTATCTCATACTAGCCACATTTGTTGGTGGAGGAATGCATCCAATGGCTGGTCACTTCATTTCAGAACATTATGTATTCAATCCTGAACAAGAGACATATTCTTACTACGGACCTTTGAATCTTCTCACATGGCATGTAGGTTACCACAATGAACACCATGATTTTCCAAGAATTCCTGGAAACAAACTTCACAAGGTGAAGGAGATTGCACCAGAATATTATGATAGTTTGTCACACTATAAATCATGGAGTCAGGTTATTTACATGTATATTATGGACAAAACAGTTGGACCTTATAGCAGAATGAAGAGAAAGCCAAGCAAAACTGAATGA
Protein sequence

>Msa0234040

MGKGGGGGGEDEEGVMMAGDFFWSYTDEPHASRRRQILSKYPQIKQLFGPDPFAFFKISVVVLLQLVTAILLKDAGWLKILLVAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRWLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGVDMDIPSHMEAHVVTNVLAKTIWVFLQLFFYAFRPLFLKPKPPGIWEFINFSVQIALDVSMVYFWGWKSLAYLILATFVGGGMHPMAGHFISEHYVFNPEQETYSYYGPLNLLTWHVGYHNEHHDFPRIPGNKLHKVKEIAPEYYDSLSHYKSWSQVIYMYIMDKTVGPYSRMKRKPSKTE*