AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192320 MtrunA17_Chr2g0282521 91.608 143 10 1 1 141 1 143 2.56e-79 230
Msa0192320 MtrunA17_Chr4g0046991 64.085 142 48 2 1 140 11 151 9.95e-52 161
Msa0192320 MtrunA17_Chr8g0360361 66.667 81 27 0 59 139 62 142 1.53e-36 123
Msa0192320 MtrunA17_Chr7g0231911 70.238 84 25 0 54 137 141 224 1.20e-35 125
Msa0192320 MtrunA17_Chr8g0334671 67.470 83 27 0 54 136 179 261 3.88e-35 124
Msa0192320 MtrunA17_Chr5g0420051 61.053 95 37 0 46 140 165 259 5.62e-35 125
Msa0192320 MtrunA17_Chr1g0162871 56.410 117 40 3 23 136 70 178 1.10e-34 127
Msa0192320 MtrunA17_Chr7g0258471 67.059 85 24 1 55 135 131 215 1.55e-33 121
Msa0192320 MtrunA17_Chr2g0302791 69.333 75 23 0 62 136 139 213 2.65e-33 123
Msa0192320 MtrunA17_Chr4g0061651 70.886 79 23 0 57 135 118 196 7.93e-33 120
Msa0192320 MtrunA17_Chr7g0270541 57.692 104 34 1 33 136 126 219 1.08e-32 121
Msa0192320 MtrunA17_Chr3g0122411 56.842 95 40 1 46 139 148 242 1.23e-32 119
Msa0192320 MtrunA17_Chr7g0224821 65.432 81 28 0 56 136 71 151 2.07e-32 117
Msa0192320 MtrunA17_Chr2g0308321 67.500 80 26 0 62 141 171 250 3.95e-32 119
Msa0192320 MtrunA17_Chr1g0194121 61.957 92 34 1 49 139 133 224 1.09e-31 118
Msa0192320 MtrunA17_Chr3g0132091 42.568 148 70 2 7 139 3 150 8.13e-31 113
Msa0192320 MtrunA17_Chr8g0386271 61.905 84 32 0 56 139 93 176 8.58e-29 108
Msa0192320 MtrunA17_Chr8g0377421 42.857 133 58 5 8 135 86 205 2.11e-25 97.8
Msa0192320 MtrunA17_Chr8g0377401 57.895 76 32 0 59 134 159 234 3.13e-25 96.7
Msa0192320 MtrunA17_Chr2g0317041 55.000 60 27 0 49 108 86 145 3.59e-19 78.6
Msa0192320 MtrunA17_Chr2g0286661 50.000 74 37 0 62 135 103 176 7.73e-19 81.6
Msa0192320 MtrunA17_Chr1g0175031 60.714 56 22 0 53 108 65 120 9.02e-17 71.6
Msa0192320 MtrunA17_Chr8g0377431 48.214 56 29 0 60 115 149 204 6.69e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192320 AT2G33810.1 70.000 90 24 1 53 139 42 131 2.50e-39 129
Msa0192320 AT1G53160.2 62.626 99 37 0 41 139 33 131 2.74e-38 128
Msa0192320 AT1G53160.1 62.626 99 37 0 41 139 33 131 2.74e-38 128
Msa0192320 AT1G53160.3 62.626 99 37 0 41 139 33 131 2.87e-38 128
Msa0192320 AT1G20980.1 68.539 89 26 1 49 137 109 195 7.14e-36 130
Msa0192320 AT1G76580.1 68.750 80 25 0 60 139 112 191 1.89e-34 126
Msa0192320 AT3G60030.1 63.830 94 29 2 50 139 112 204 4.39e-34 125
Msa0192320 AT1G02065.1 68.293 82 26 0 54 135 180 261 4.56e-34 121
Msa0192320 AT1G76580.3 68.750 80 25 0 60 139 112 191 8.65e-34 124
Msa0192320 AT3G15270.1 65.823 79 25 1 59 135 58 136 1.03e-33 116
Msa0192320 AT1G76580.2 68.750 80 25 0 60 139 112 191 1.09e-33 124
Msa0192320 AT2G47070.1 60.825 97 34 1 43 139 91 183 1.33e-33 124
Msa0192320 AT1G69170.1 70.886 79 23 0 57 135 119 197 4.73e-33 120
Msa0192320 AT1G27370.6 55.670 97 41 1 41 135 153 249 1.81e-31 115
Msa0192320 AT1G27370.1 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0192320 AT1G27370.5 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0192320 AT1G27370.3 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0192320 AT1G27370.2 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0192320 AT1G27370.4 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0192320 AT1G27370.7 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0192320 AT1G27360.2 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0192320 AT1G27360.1 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0192320 AT1G27360.3 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0192320 AT1G27360.4 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0192320 AT3G57920.1 56.731 104 40 1 32 135 34 132 2.12e-30 112
Msa0192320 AT5G43270.3 64.103 78 28 0 59 136 166 243 3.48e-30 112
Msa0192320 AT5G43270.1 64.103 78 28 0 59 136 166 243 3.48e-30 112
Msa0192320 AT5G43270.2 64.103 78 28 0 59 136 166 243 3.48e-30 112
Msa0192320 AT2G42200.1 63.636 77 28 0 60 136 72 148 4.42e-30 112
Msa0192320 AT5G50570.1 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0192320 AT5G50570.2 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0192320 AT5G50670.1 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0192320 AT5G50670.2 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0192320 AT1G02065.2 61.538 65 23 1 54 116 180 244 7.11e-21 85.1
Msa0192320 AT5G18830.2 49.383 81 41 0 55 135 131 211 3.35e-20 85.9
Msa0192320 AT5G18830.1 49.383 81 41 0 55 135 131 211 3.88e-20 85.9
Msa0192320 AT5G18830.3 49.383 81 41 0 55 135 131 211 4.29e-20 85.5
Msa0192320 AT2G47070.2 67.925 53 17 0 87 139 2 54 3.86e-19 82.8

Find 71 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATTTACCTTAACTTA+TGG 0.220696 2_1:-8067949 Msa0192320:exon
ACAAAAGGGTGCTCATTTAT+TGG 0.224690 2_1:+8068090 None:intergenic
CACAAACCTTATGGCGCTTA+TGG 0.248219 2_1:+8067700 None:intergenic
ATATCCCTCAAATATATTAT+TGG 0.277920 2_1:+8065538 None:intergenic
AATGGTATTCACAAACCTTA+TGG 0.308780 2_1:+8067691 None:intergenic
GAAGAGGAAGATGTGAGTTA+TGG 0.319891 2_1:-8067855 Msa0192320:CDS
AAGCCATGGTAATAAATTAA+TGG 0.327177 2_1:+8065566 None:intergenic
AAGAGGAAGATGTGAGTTAT+GGG 0.331465 2_1:-8067854 Msa0192320:CDS
ATGACTTGAAAAGGAGTTGT+AGG 0.355530 2_1:-8065883 Msa0192320:CDS
TTAACTTATGGATGGAAGTT+GGG 0.358056 2_1:-8067937 Msa0192320:exon
GGTTTGTGAATACCATTCTA+AGG 0.368542 2_1:-8067685 Msa0192320:CDS
TTGTCAACCTGCTACATTGC+TGG 0.372075 2_1:+8067622 None:intergenic
CTTAACTTATGGATGGAAGT+TGG 0.386744 2_1:-8067938 Msa0192320:exon
ATTCAAATTGATGTTATTTG+TGG 0.399031 2_1:-8065731 Msa0192320:three_prime_UTR
CTCACCAATAATATATTTGA+GGG 0.417935 2_1:-8065542 Msa0192320:three_prime_UTR
CAGAATTTGATGACTTGAAA+AGG 0.424158 2_1:-8065892 Msa0192320:CDS
CAACTTCCATCCATAAGTTA+AGG 0.431263 2_1:+8067939 None:intergenic
AAGAGGGTGTGGACCACAAA+AGG 0.442762 2_1:+8068075 None:intergenic
TCTCACCAATAATATATTTG+AGG 0.442874 2_1:-8065543 Msa0192320:three_prime_UTR
GGGATGATGGGAAGAAGAAA+AGG 0.444953 2_1:-8067833 Msa0192320:CDS
AAGGAGTTGTAGGAGGCGTT+TGG 0.456087 2_1:-8065873 Msa0192320:CDS
TGAAGTAGAAGTAGAAGAAG+AGG 0.463169 2_1:-8067871 Msa0192320:CDS
ATGGATGGAAGTTGGGGTGA+GGG 0.465253 2_1:-8067930 Msa0192320:CDS
TTTATTGGTTGCCAACTTGA+GGG 0.465401 2_1:+8068105 None:intergenic
AAAAGGGTAAATAAATAGAA+TGG 0.469519 2_1:+8068049 None:intergenic
GATGTGAGTTATGGGGATGA+TGG 0.475010 2_1:-8067846 Msa0192320:CDS
AACAGGAAAGGGTCAAAAGC+TGG 0.479614 2_1:-8067789 Msa0192320:CDS
CTTCTATACCAAGGTTGTTG+TGG 0.480026 2_1:-8068014 Msa0192320:five_prime_UTR
AGTTGTAGGAGGCGTTTGGC+TGG 0.487448 2_1:-8065869 Msa0192320:CDS
ATTTATTGGTTGCCAACTTG+AGG 0.488594 2_1:+8068104 None:intergenic
TAACATCAATTTGAATATGA+AGG 0.494767 2_1:+8065738 None:intergenic
TATGGATGGAAGTTGGGGTG+AGG 0.500828 2_1:-8067931 Msa0192320:CDS
TGACTGATCATCTTTACAAC+AGG 0.501584 2_1:-8067806 Msa0192320:CDS
GTTATTAAAGAATGAAAAGA+GGG 0.503992 2_1:+8067974 None:intergenic
CAACTTGAGGGTAACAGTGA+TGG 0.504518 2_1:+8068117 None:intergenic
TGAAAAGAGGGTGAAGTCAA+GGG 0.507320 2_1:+8067986 None:intergenic
ATGAAAAGAGGGTGAAGTCA+AGG 0.509681 2_1:+8067985 None:intergenic
ATCATCTTTACAACAGGAAA+GGG 0.510630 2_1:-8067800 Msa0192320:CDS
TTGAAGCTCTGAAATGAGTA+CGG 0.530837 2_1:+8067656 None:intergenic
GCAGTACCATAAGCGCCATA+AGG 0.534642 2_1:-8067706 Msa0192320:CDS
AGAGGAAGATGTGAGTTATG+GGG 0.544242 2_1:-8067853 Msa0192320:CDS
GATTTACCTTAACTTATGGA+TGG 0.546093 2_1:-8067945 Msa0192320:exon
ATGTGAGTTATGGGGATGAT+GGG 0.550503 2_1:-8067845 Msa0192320:CDS
GGATGATGGGAAGAAGAAAA+GGG 0.556520 2_1:-8067832 Msa0192320:CDS
GGTAATAAATTAATGGTACA+AGG 0.560402 2_1:+8065573 None:intergenic
AGTTATTAAAGAATGAAAAG+AGG 0.561099 2_1:+8067973 None:intergenic
GCAGCAAACTTAGCATAAAG+AGG 0.566131 2_1:+8065674 None:intergenic
TAACTTATGGATGGAAGTTG+GGG 0.568515 2_1:-8067936 Msa0192320:exon
GATCATCTTTACAACAGGAA+AGG 0.569404 2_1:-8067801 Msa0192320:CDS
GAAAAGAGGGTGAAGTCAAG+GGG 0.570578 2_1:+8067987 None:intergenic
GTACGGAATGAGCCTTAGAA+TGG 0.572289 2_1:+8067673 None:intergenic
GTACCATTAATTTATTACCA+TGG 0.575456 2_1:-8065569 Msa0192320:three_prime_UTR
CAGTTATCAACTTGACAAGA+AGG 0.576877 2_1:+8067753 None:intergenic
ATTTGAATATGAAGGACTCA+AGG 0.580481 2_1:+8065746 None:intergenic
CACATACAATACAATTGCAA+AGG 0.586026 2_1:+8065608 None:intergenic
AACTTGAGGGTAACAGTGAT+GGG 0.587158 2_1:+8068118 None:intergenic
ATAGAGCATTAGCTGAGGGC+CGG 0.588404 2_1:+8068152 None:intergenic
ACGTCTGAATATCACTGAGA+AGG 0.591085 2_1:-8065821 Msa0192320:exon
TCATTTCAGAGCTTCAACAA+AGG 0.599380 2_1:-8067650 Msa0192320:CDS
AGAGGGTGTGGACCACAAAA+GGG 0.600294 2_1:+8068076 None:intergenic
GGGGAAGACCACAACAACCT+TGG 0.600711 2_1:+8068006 None:intergenic
CATCACTGTTACCCTCAAGT+TGG 0.601011 2_1:-8068116 Msa0192320:five_prime_UTR
CAGCAAACTTAGCATAAAGA+GGG 0.607629 2_1:+8065675 None:intergenic
ACTTGAAAAGGAGTTGTAGG+AGG 0.645868 2_1:-8065880 Msa0192320:CDS
CTATATAGAGCATTAGCTGA+GGG 0.646069 2_1:+8068148 None:intergenic
TATTATTGGTGAGAAAGCCA+TGG 0.646118 2_1:+8065552 None:intergenic
TGATGGGAAGAAGAAAAGGG+TGG 0.662923 2_1:-8067829 Msa0192320:CDS
AGGAAAGGGTCAAAAGCTGG+AGG 0.666819 2_1:-8067786 Msa0192320:CDS
ACTATATAGAGCATTAGCTG+AGG 0.670283 2_1:+8068147 None:intergenic
CATAAGTTAAGGTAAATCTG+AGG 0.674591 2_1:+8067950 None:intergenic
GTTTGGCTGGACATAACGAG+AGG 0.681910 2_1:-8065856 Msa0192320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTCTATCAAATA+AGG - chr2_1:8066275-8066294 Msa0192320:intron 10.0%
!! ATATTAATATTTACTAGAAA+TGG + chr2_1:8066333-8066352 None:intergenic 10.0%
!!! TTATTTTTTAATATTCCAAT+AGG - chr2_1:8066457-8066476 Msa0192320:intron 10.0%
!!! AAAATGATAGATTTTTATAA+GGG + chr2_1:8066559-8066578 None:intergenic 10.0%
!!! TAAAATGATAGATTTTTATA+AGG + chr2_1:8066560-8066579 None:intergenic 10.0%
!!! AAAAAAAAACTTGTTTTATA+TGG + chr2_1:8066626-8066645 None:intergenic 10.0%
!!! AAACAAGTTTTTTTTTAATA+TGG - chr2_1:8066630-8066649 Msa0192320:intron 10.0%
!!! ATTTTGTAAAAATAAGATTA+AGG - chr2_1:8066711-8066730 Msa0192320:intron 10.0%
!!! TTTTGTAAAAATAAGATTAA+GGG - chr2_1:8066712-8066731 Msa0192320:intron 10.0%
!!! ATTTCATCATTATAATTTTT+TGG - chr2_1:8066798-8066817 Msa0192320:intron 10.0%
!!! TTTTTTATTGATAAATACCT+TGG - chr2_1:8066193-8066212 Msa0192320:intron 15.0%
!!! TCATTTTACTAAAAATAGTA+AGG - chr2_1:8066572-8066591 Msa0192320:intron 15.0%
!!! CATTTTACTAAAAATAGTAA+GGG - chr2_1:8066573-8066592 Msa0192320:intron 15.0%
!!! TTTATATGGACTTTTAAAAA+AGG + chr2_1:8066612-8066631 None:intergenic 15.0%
!! ATTAATTAGTTGTAAATCAT+GGG + chr2_1:8067694-8067713 None:intergenic 15.0%
!! AATTAATTAGTTGTAAATCA+TGG + chr2_1:8067695-8067714 None:intergenic 15.0%
!! TAATTATTAACTTATAGCTT+TGG - chr2_1:8067710-8067729 Msa0192320:CDS 15.0%
!! ATAAATAGAATGGAAAAAGA+GGG + chr2_1:8065601-8065620 None:intergenic 20.0%
!! AATAAATAGAATGGAAAAAG+AGG + chr2_1:8065602-8065621 None:intergenic 20.0%
!! AAAAGGGTAAATAAATAGAA+TGG + chr2_1:8065611-8065630 None:intergenic 20.0%
!! GAAGTAGAAAAAACATAAAA+GGG + chr2_1:8065627-8065646 None:intergenic 20.0%
!! AGAAGTAGAAAAAACATAAA+AGG + chr2_1:8065628-8065647 None:intergenic 20.0%
!! GTTATTAAAGAATGAAAAGA+GGG + chr2_1:8065686-8065705 None:intergenic 20.0%
!! AGTTATTAAAGAATGAAAAG+AGG + chr2_1:8065687-8065706 None:intergenic 20.0%
!! AAGATATTCAAGGAAAAAAA+AGG + chr2_1:8066073-8066092 None:intergenic 20.0%
!! TACTAATACTAAGATATTCA+AGG + chr2_1:8066083-8066102 None:intergenic 20.0%
!! AAATATTGAGGTTCAATATA+AGG + chr2_1:8066115-8066134 None:intergenic 20.0%
!! TTTAAGCTTCTTAACAAATA+AGG - chr2_1:8066511-8066530 Msa0192320:intron 20.0%
!! TTAAGCTTCTTAACAAATAA+GGG - chr2_1:8066512-8066531 Msa0192320:intron 20.0%
!! TCAAAAAAAATGTTCAAATG+TGG - chr2_1:8066656-8066675 Msa0192320:intron 20.0%
!!! TTTTACTATAAAAGAGGTAA+TGG + chr2_1:8066683-8066702 None:intergenic 20.0%
!!! ACCTCTTTTATAGTAAAATA+TGG - chr2_1:8066685-8066704 Msa0192320:intron 20.0%
!!! ACCATATTTTACTATAAAAG+AGG + chr2_1:8066689-8066708 None:intergenic 20.0%
!! ATAAGATTAAGGGTAAAATA+CGG - chr2_1:8066722-8066741 Msa0192320:intron 20.0%
!! AAAAATAAAATAAAGGGAAG+TGG - chr2_1:8066755-8066774 Msa0192320:intron 20.0%
!! TGAACATCAATAAATATTCA+GGG + chr2_1:8067133-8067152 None:intergenic 20.0%
!! ATGAACATCAATAAATATTC+AGG + chr2_1:8067134-8067153 None:intergenic 20.0%
!!! CATGAAGTATCTTTAAAAAA+TGG + chr2_1:8067438-8067457 None:intergenic 20.0%
!!! CATTTTTTAAAGATACTTCA+TGG - chr2_1:8067436-8067455 Msa0192320:intron 20.0%
!!! TTTTTTTTTGAAGAAGCATA+AGG + chr2_1:8067584-8067603 None:intergenic 20.0%
!! TAACATCAATTTGAATATGA+AGG + chr2_1:8067922-8067941 None:intergenic 20.0%
!!! ATTCAAATTGATGTTATTTG+TGG - chr2_1:8067926-8067945 Msa0192320:CDS 20.0%
!! ATATCCCTCAAATATATTAT+TGG + chr2_1:8068122-8068141 None:intergenic 20.0%
!!! TTTTTTCTACTTCTATACCA+AGG - chr2_1:8065634-8065653 Msa0192320:three_prime_UTR 25.0%
! GGAAAAAAAAGGTGAAATTA+GGG + chr2_1:8066062-8066081 None:intergenic 25.0%
! AGGAAAAAAAAGGTGAAATT+AGG + chr2_1:8066063-8066082 None:intergenic 25.0%
!!! CACTTTTGAACATTTTTTAG+AGG + chr2_1:8066302-8066321 None:intergenic 25.0%
! TTTGTGCATCATTATAAACT+GGG - chr2_1:8066367-8066386 Msa0192320:intron 25.0%
! ATGCTAACAATTGTATGTTA+AGG - chr2_1:8066392-8066411 Msa0192320:intron 25.0%
! GAAAATTGTAATCAACCTAT+TGG + chr2_1:8066475-8066494 None:intergenic 25.0%
!!! TAGGTTGATTACAATTTTCA+TGG - chr2_1:8066476-8066495 Msa0192320:intron 25.0%
! TTCTTAACAAATAAGGGTTA+TGG - chr2_1:8066518-8066537 Msa0192320:intron 25.0%
!!! TCATATTAGATGCACTTTTT+TGG - chr2_1:8066824-8066843 Msa0192320:intron 25.0%
!! ACCAACAAATTTTTCCAATT+GGG + chr2_1:8067088-8067107 None:intergenic 25.0%
!! TACCAACAAATTTTTCCAAT+TGG + chr2_1:8067089-8067108 None:intergenic 25.0%
! GAACATCAATAAATATTCAG+GGG + chr2_1:8067132-8067151 None:intergenic 25.0%
! ATATATGATCTTGAAGTTGA+AGG + chr2_1:8067278-8067297 None:intergenic 25.0%
!! CTTTAGTCATCACTTAATTT+TGG - chr2_1:8067360-8067379 Msa0192320:intron 25.0%
!!! TTTTTAAAGATACTTCATGG+AGG - chr2_1:8067439-8067458 Msa0192320:intron 25.0%
! GACAGGAAGAAAAAAAATTA+AGG - chr2_1:8067465-8067484 Msa0192320:intron 25.0%
!! AGAACATTGTGTATATGTTT+TGG + chr2_1:8067639-8067658 None:intergenic 25.0%
! ACACAATGTTCTTCATATAT+AGG - chr2_1:8067647-8067666 Msa0192320:CDS 25.0%
!!! TAACTTATAGCTTTGGAATA+TGG - chr2_1:8067717-8067736 Msa0192320:CDS 25.0%
!! TTGGAATATGGTTTTTCTAT+AGG - chr2_1:8067729-8067748 Msa0192320:CDS 25.0%
! GGTAATAAATTAATGGTACA+AGG + chr2_1:8068087-8068106 None:intergenic 25.0%
! GTACCATTAATTTATTACCA+TGG - chr2_1:8068088-8068107 Msa0192320:five_prime_UTR 25.0%
! AAGCCATGGTAATAAATTAA+TGG + chr2_1:8068094-8068113 None:intergenic 25.0%
! TCTCACCAATAATATATTTG+AGG - chr2_1:8068114-8068133 Msa0192320:five_prime_UTR 25.0%
! CTCACCAATAATATATTTGA+GGG - chr2_1:8068115-8068134 Msa0192320:five_prime_UTR 25.0%
CATAAGTTAAGGTAAATCTG+AGG + chr2_1:8065710-8065729 None:intergenic 30.0%
CTCAGATTTACCTTAACTTA+TGG - chr2_1:8065708-8065727 Msa0192320:three_prime_UTR 30.0%
GATTTACCTTAACTTATGGA+TGG - chr2_1:8065712-8065731 Msa0192320:three_prime_UTR 30.0%
TTAACTTATGGATGGAAGTT+GGG - chr2_1:8065720-8065739 Msa0192320:three_prime_UTR 30.0%
ATCATCTTTACAACAGGAAA+GGG - chr2_1:8065857-8065876 Msa0192320:CDS 30.0%
AATGGTATTCACAAACCTTA+TGG + chr2_1:8065969-8065988 None:intergenic 30.0%
CTTAGTTATTAGTAAGACCA+AGG + chr2_1:8066213-8066232 None:intergenic 30.0%
CTTTGTGCATCATTATAAAC+TGG - chr2_1:8066366-8066385 Msa0192320:intron 30.0%
TTTAAAAAAGGAGCCAAATG+TGG + chr2_1:8066600-8066619 None:intergenic 30.0%
!!! TTAGATGCACTTTTTTGGAT+AGG - chr2_1:8066829-8066848 Msa0192320:intron 30.0%
TTTGTAGGAGTGAAAATCAA+AGG + chr2_1:8066974-8066993 None:intergenic 30.0%
!! TTTGATTTTCACTCCTACAA+AGG - chr2_1:8066973-8066992 Msa0192320:intron 30.0%
AAGTTTAGCACTATATAGCT+AGG + chr2_1:8067009-8067028 None:intergenic 30.0%
!! TTGGAAAAATTTGTTGGTAC+AGG - chr2_1:8067090-8067109 Msa0192320:intron 30.0%
AAATGCTATTCGTCTATAGT+TGG + chr2_1:8067337-8067356 None:intergenic 30.0%
! CAAATTTGAGACTTAAACGT+TGG - chr2_1:8067531-8067550 Msa0192320:intron 30.0%
! GTTTTTCTATAGGTTTCATG+AGG - chr2_1:8067739-8067758 Msa0192320:CDS 30.0%
! CAGAATTTGATGACTTGAAA+AGG - chr2_1:8067765-8067784 Msa0192320:CDS 30.0%
ATTTGAATATGAAGGACTCA+AGG + chr2_1:8067914-8067933 None:intergenic 30.0%
CACATACAATACAATTGCAA+AGG + chr2_1:8068052-8068071 None:intergenic 30.0%
! CTATATAGAGCATTAGCTGA+GGG + chr2_1:8065512-8065531 None:intergenic 35.0%
! ACTATATAGAGCATTAGCTG+AGG + chr2_1:8065513-8065532 None:intergenic 35.0%
! TTTATTGGTTGCCAACTTGA+GGG + chr2_1:8065555-8065574 None:intergenic 35.0%
! ATTTATTGGTTGCCAACTTG+AGG + chr2_1:8065556-8065575 None:intergenic 35.0%
!! ACAAAAGGGTGCTCATTTAT+TGG + chr2_1:8065570-8065589 None:intergenic 35.0%
! AATAAATGAGCACCCTTTTG+TGG - chr2_1:8065569-8065588 Msa0192320:three_prime_UTR 35.0%
CAACTTCCATCCATAAGTTA+AGG + chr2_1:8065721-8065740 None:intergenic 35.0%
CTTAACTTATGGATGGAAGT+TGG - chr2_1:8065719-8065738 Msa0192320:three_prime_UTR 35.0%
TAACTTATGGATGGAAGTTG+GGG - chr2_1:8065721-8065740 Msa0192320:three_prime_UTR 35.0%
TGAAGTAGAAGTAGAAGAAG+AGG - chr2_1:8065786-8065805 Msa0192320:three_prime_UTR 35.0%
AAGAGGAAGATGTGAGTTAT+GGG - chr2_1:8065803-8065822 Msa0192320:three_prime_UTR 35.0%
TGACTGATCATCTTTACAAC+AGG - chr2_1:8065851-8065870 Msa0192320:CDS 35.0%
GATCATCTTTACAACAGGAA+AGG - chr2_1:8065856-8065875 Msa0192320:CDS 35.0%
CAGTTATCAACTTGACAAGA+AGG + chr2_1:8065907-8065926 None:intergenic 35.0%
GGTTTGTGAATACCATTCTA+AGG - chr2_1:8065972-8065991 Msa0192320:intron 35.0%
!! TTGAAGCTCTGAAATGAGTA+CGG + chr2_1:8066004-8066023 None:intergenic 35.0%
TCATTTCAGAGCTTCAACAA+AGG - chr2_1:8066007-8066026 Msa0192320:intron 35.0%
CCACTTCACATCAAATATTG+AGG + chr2_1:8066127-8066146 None:intergenic 35.0%
CCTCAATATTTGATGTGAAG+TGG - chr2_1:8066124-8066143 Msa0192320:intron 35.0%
AAATAGTAAGGGTCCACATT+TGG - chr2_1:8066584-8066603 Msa0192320:intron 35.0%
GGAAAATGTCTGTAAGTTAC+TGG - chr2_1:8066850-8066869 Msa0192320:intron 35.0%
AGGCTAACTATATCCTTTGT+AGG + chr2_1:8066989-8067008 None:intergenic 35.0%
GCCCAATTGGAAAAATTTGT+TGG - chr2_1:8067084-8067103 Msa0192320:intron 35.0%
AGCATGTTGGGGTAATTAAA+AGG + chr2_1:8067229-8067248 None:intergenic 35.0%
! TATAGCAGTGTTACTGTGAT+TGG - chr2_1:8067297-8067316 Msa0192320:intron 35.0%
! TTTTGTGTTGTCAACTTCGT+GGG + chr2_1:8067402-8067421 None:intergenic 35.0%
GACACAGGTTAAAACTGATT+TGG + chr2_1:8067562-8067581 None:intergenic 35.0%
TGGTTAATTTAGCATGCACA+AGG + chr2_1:8067619-8067638 None:intergenic 35.0%
ATGACTTGAAAAGGAGTTGT+AGG - chr2_1:8067774-8067793 Msa0192320:CDS 35.0%
CAGCAAACTTAGCATAAAGA+GGG + chr2_1:8067985-8068004 None:intergenic 35.0%
TATTATTGGTGAGAAAGCCA+TGG + chr2_1:8068108-8068127 None:intergenic 35.0%
AACTTGAGGGTAACAGTGAT+GGG + chr2_1:8065542-8065561 None:intergenic 40.0%
TAGAATGGAAAAAGAGGGTG+TGG + chr2_1:8065596-8065615 None:intergenic 40.0%
CTTCTATACCAAGGTTGTTG+TGG - chr2_1:8065643-8065662 Msa0192320:three_prime_UTR 40.0%
TGAAAAGAGGGTGAAGTCAA+GGG + chr2_1:8065674-8065693 None:intergenic 40.0%
ATGAAAAGAGGGTGAAGTCA+AGG + chr2_1:8065675-8065694 None:intergenic 40.0%
GAAGAGGAAGATGTGAGTTA+TGG - chr2_1:8065802-8065821 Msa0192320:three_prime_UTR 40.0%
AGAGGAAGATGTGAGTTATG+GGG - chr2_1:8065804-8065823 Msa0192320:three_prime_UTR 40.0%
ATGTGAGTTATGGGGATGAT+GGG - chr2_1:8065812-8065831 Msa0192320:exon 40.0%
!! GGATGATGGGAAGAAGAAAA+GGG - chr2_1:8065825-8065844 Msa0192320:exon 40.0%
TATTTGATGTGAAGTGGCTG+AGG - chr2_1:8066130-8066149 Msa0192320:intron 40.0%
ATTTGATGTGAAGTGGCTGA+GGG - chr2_1:8066131-8066150 Msa0192320:intron 40.0%
CTATGAGTTGTCTCTTGCTA+GGG + chr2_1:8066236-8066255 None:intergenic 40.0%
GATTTAGCAACTTGCCCAAT+TGG - chr2_1:8067071-8067090 Msa0192320:intron 40.0%
TACATAGAACTCACACTCAC+AGG - chr2_1:8067168-8067187 Msa0192320:intron 40.0%
ACATAGAACTCACACTCACA+GGG - chr2_1:8067169-8067188 Msa0192320:intron 40.0%
TTTGTGTTGTCAACTTCGTG+GGG + chr2_1:8067401-8067420 None:intergenic 40.0%
! CTTTTGTGTTGTCAACTTCG+TGG + chr2_1:8067403-8067422 None:intergenic 40.0%
CACGAAGTTGACAACACAAA+AGG - chr2_1:8067401-8067420 Msa0192320:intron 40.0%
! TTGAAGAAGCATAAGGACAC+AGG + chr2_1:8067577-8067596 None:intergenic 40.0%
ACTTGAAAAGGAGTTGTAGG+AGG - chr2_1:8067777-8067796 Msa0192320:CDS 40.0%
! GATATTCAGACGTGCTTTTG+CGG + chr2_1:8067829-8067848 None:intergenic 40.0%
ACGTCTGAATATCACTGAGA+AGG - chr2_1:8067836-8067855 Msa0192320:CDS 40.0%
GCAGCAAACTTAGCATAAAG+AGG + chr2_1:8067986-8068005 None:intergenic 40.0%
CAACTTGAGGGTAACAGTGA+TGG + chr2_1:8065543-8065562 None:intergenic 45.0%
CATCACTGTTACCCTCAAGT+TGG - chr2_1:8065541-8065560 Msa0192320:three_prime_UTR 45.0%
GAAAAGAGGGTGAAGTCAAG+GGG + chr2_1:8065673-8065692 None:intergenic 45.0%
GATGTGAGTTATGGGGATGA+TGG - chr2_1:8065811-8065830 Msa0192320:exon 45.0%
!! GGGATGATGGGAAGAAGAAA+AGG - chr2_1:8065824-8065843 Msa0192320:exon 45.0%
TGATGGGAAGAAGAAAAGGG+TGG - chr2_1:8065828-8065847 Msa0192320:CDS 45.0%
AACAGGAAAGGGTCAAAAGC+TGG - chr2_1:8065868-8065887 Msa0192320:CDS 45.0%
CACAAACCTTATGGCGCTTA+TGG + chr2_1:8065960-8065979 None:intergenic 45.0%
GTACGGAATGAGCCTTAGAA+TGG + chr2_1:8065987-8066006 None:intergenic 45.0%
TTGTCAACCTGCTACATTGC+TGG + chr2_1:8066038-8066057 None:intergenic 45.0%
CCTATGAGTTGTCTCTTGCT+AGG + chr2_1:8066237-8066256 None:intergenic 45.0%
CCTAGCAAGAGACAACTCAT+AGG - chr2_1:8066234-8066253 Msa0192320:intron 45.0%
TTACCCCAACATGCTGAGAT+AGG - chr2_1:8067234-8067253 Msa0192320:intron 45.0%
TACCCCAACATGCTGAGATA+GGG - chr2_1:8067235-8067254 Msa0192320:intron 45.0%
TGTCCCTATCTCAGCATGTT+GGG + chr2_1:8067241-8067260 None:intergenic 45.0%
ATGTCCCTATCTCAGCATGT+TGG + chr2_1:8067242-8067261 None:intergenic 45.0%
ATACTTCATGGAGGCAAGAC+AGG - chr2_1:8067448-8067467 Msa0192320:intron 45.0%
!!! ATTTTGTAAAAATAAAATAA+AGG - chr2_1:8066748-8066767 Msa0192320:intron 5.0%
!!! TTTTGTAAAAATAAAATAAA+GGG - chr2_1:8066749-8066768 Msa0192320:intron 5.0%
! ATAGAGCATTAGCTGAGGGC+CGG + chr2_1:8065508-8065527 None:intergenic 50.0%
! AGAGGGTGTGGACCACAAAA+GGG + chr2_1:8065584-8065603 None:intergenic 50.0%
! AAGAGGGTGTGGACCACAAA+AGG + chr2_1:8065585-8065604 None:intergenic 50.0%
TATGGATGGAAGTTGGGGTG+AGG - chr2_1:8065726-8065745 Msa0192320:three_prime_UTR 50.0%
ATGGATGGAAGTTGGGGTGA+GGG - chr2_1:8065727-8065746 Msa0192320:three_prime_UTR 50.0%
AGGAAAGGGTCAAAAGCTGG+AGG - chr2_1:8065871-8065890 Msa0192320:CDS 50.0%
GCAGTACCATAAGCGCCATA+AGG - chr2_1:8065951-8065970 Msa0192320:intron 50.0%
! GGTTTTGCCAGCAATGTAGC+AGG - chr2_1:8066028-8066047 Msa0192320:intron 50.0%
GTCCCTATCTCAGCATGTTG+GGG + chr2_1:8067240-8067259 None:intergenic 50.0%
!! AAGGAGTTGTAGGAGGCGTT+TGG - chr2_1:8067784-8067803 Msa0192320:CDS 50.0%
GTTTGGCTGGACATAACGAG+AGG - chr2_1:8067801-8067820 Msa0192320:CDS 50.0%
GGGGAAGACCACAACAACCT+TGG + chr2_1:8065654-8065673 None:intergenic 55.0%
!! AGTTGTAGGAGGCGTTTGGC+TGG - chr2_1:8067788-8067807 Msa0192320:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8065503 8068176 8065503 ID=Msa0192320;Name=Msa0192320
chr2_1 mRNA 8065503 8068176 8065503 ID=Msa0192320-mRNA-1;Parent=Msa0192320;Name=Msa0192320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=224|1|1|1|1|1|2|323|141
chr2_1 exon 8065503 8065928 8065503 ID=Msa0192320-mRNA-1:exon:3718;Parent=Msa0192320-mRNA-1
chr2_1 exon 8067630 8068176 8067630 ID=Msa0192320-mRNA-1:exon:3717;Parent=Msa0192320-mRNA-1
chr2_1 five_prime_UTR 8067953 8068176 8067953 ID=Msa0192320-mRNA-1:five_prime_utr;Parent=Msa0192320-mRNA-1
chr2_1 CDS 8067630 8067952 8067630 ID=Msa0192320-mRNA-1:cds;Parent=Msa0192320-mRNA-1
chr2_1 CDS 8065826 8065928 8065826 ID=Msa0192320-mRNA-1:cds;Parent=Msa0192320-mRNA-1
chr2_1 three_prime_UTR 8065503 8065825 8065503 ID=Msa0192320-mRNA-1:three_prime_utr;Parent=Msa0192320-mRNA-1
Gene Sequence

>Msa0192320

ATGGATGGAAGTTGGGGTGAGGGAAAGAGAATCTATGAGTACAGAGAAGAAGATGAGTATGAAGTAGAAGTAGAAGAAGAGGAAGATGTGAGTTATGGGGATGATGGGAAGAAGAAAAGGGTGGTGACTGATCATCTTTACAACAGGAAAGGGTCAAAAGCTGGAGGATCAGTGACACCTTCTTGTCAAGTTGATAACTGTAATGCTGATCTAAGTGTTGCTAAGCAGTACCATAAGCGCCATAAGGTTTGTGAATACCATTCTAAGGCTCATTCCGTACTCATTTCAGAGCTTCAACAAAGGTTTTGCCAGCAATGTAGCAGGTTTCATGAGGTATCAGAATTTGATGACTTGAAAAGGAGTTGTAGGAGGCGTTTGGCTGGACATAACGAGAGGCGCCGCAAAAGCACGTCTGAATATCACTGA
Protein sequence

>Msa0192320

MDGSWGEGKRIYEYREEDEYEVEVEEEEDVSYGDDGKKKRVVTDHLYNRKGSKAGGSVTPSCQVDNCNADLSVAKQYHKRHKVCEYHSKAHSVLISELQQRFCQQCSRFHEVSEFDDLKRSCRRRLAGHNERRRKSTSEYH*