AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0313080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313080 MtrunA17_Chr2g0282521 91.608 143 10 1 33 173 1 143 1.14e-78 230
Msa0313080 MtrunA17_Chr4g0046991 64.085 142 48 2 33 172 11 151 5.45e-51 160
Msa0313080 MtrunA17_Chr8g0360361 67.500 80 26 0 92 171 63 142 1.20e-35 122
Msa0313080 MtrunA17_Chr7g0231911 70.238 84 25 0 86 169 141 224 3.79e-35 125
Msa0313080 MtrunA17_Chr8g0334671 67.470 83 27 0 86 168 179 261 1.56e-34 123
Msa0313080 MtrunA17_Chr5g0420051 61.053 95 37 0 78 172 165 259 4.66e-34 124
Msa0313080 MtrunA17_Chr1g0162871 57.018 114 44 2 55 168 70 178 5.07e-34 126
Msa0313080 MtrunA17_Chr7g0258471 67.059 85 24 1 87 167 131 215 5.19e-33 121
Msa0313080 MtrunA17_Chr7g0270541 56.075 107 37 1 65 171 126 222 1.31e-32 122
Msa0313080 MtrunA17_Chr4g0061651 70.886 79 23 0 89 167 118 196 3.23e-32 119
Msa0313080 MtrunA17_Chr3g0122411 53.509 114 51 2 60 171 129 242 3.49e-32 119
Msa0313080 MtrunA17_Chr7g0224821 66.250 80 27 0 88 167 71 150 5.61e-32 117
Msa0313080 MtrunA17_Chr1g0194121 61.957 92 34 1 81 171 133 224 1.02e-31 120
Msa0313080 MtrunA17_Chr2g0308321 67.500 80 26 0 94 173 171 250 1.10e-31 119
Msa0313080 MtrunA17_Chr2g0302791 56.436 101 34 1 68 168 123 213 1.42e-31 119
Msa0313080 MtrunA17_Chr3g0132091 62.651 83 31 0 89 171 68 150 3.40e-30 112
Msa0313080 MtrunA17_Chr8g0386271 61.905 84 32 0 88 171 93 176 1.34e-28 108
Msa0313080 MtrunA17_Chr8g0377421 57.895 76 32 0 92 167 130 205 6.64e-25 97.8
Msa0313080 MtrunA17_Chr8g0377401 57.895 76 32 0 91 166 159 234 1.61e-24 95.9
Msa0313080 MtrunA17_Chr2g0317041 55.000 60 27 0 81 140 86 145 5.99e-19 79.0
Msa0313080 MtrunA17_Chr2g0286661 50.000 74 37 0 94 167 103 176 6.22e-19 83.2
Msa0313080 MtrunA17_Chr1g0175031 60.714 56 22 0 85 140 65 120 1.72e-16 72.0
Msa0313080 MtrunA17_Chr8g0377431 48.214 56 29 0 92 147 149 204 1.36e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313080 AT2G33810.1 70.000 90 24 1 85 171 42 131 8.07e-39 129
Msa0313080 AT1G53160.2 62.626 99 37 0 73 171 33 131 1.16e-37 127
Msa0313080 AT1G53160.1 62.626 99 37 0 73 171 33 131 1.16e-37 127
Msa0313080 AT1G53160.3 62.626 99 37 0 73 171 33 131 1.29e-37 127
Msa0313080 AT1G20980.1 68.539 89 26 1 81 169 109 195 2.23e-34 127
Msa0313080 AT3G60030.1 63.830 94 29 2 82 171 112 204 1.46e-33 125
Msa0313080 AT1G02065.1 71.429 77 22 0 91 167 185 261 1.84e-33 121
Msa0313080 AT1G76580.2 71.429 77 22 0 92 168 112 188 3.32e-33 124
Msa0313080 AT1G76580.3 71.429 77 22 0 92 168 112 188 3.35e-33 124
Msa0313080 AT2G47070.1 60.825 97 34 1 75 171 91 183 4.07e-33 124
Msa0313080 AT3G15270.1 67.568 74 24 0 94 167 63 136 4.47e-33 116
Msa0313080 AT1G76580.1 71.429 77 22 0 92 168 112 188 4.90e-33 124
Msa0313080 AT1G69170.1 67.045 88 27 1 82 167 110 197 1.84e-32 120
Msa0313080 AT1G27370.6 55.670 97 41 1 73 167 153 249 2.97e-31 115
Msa0313080 AT1G27370.1 55.670 97 41 1 73 167 153 249 6.66e-31 115
Msa0313080 AT1G27370.5 55.670 97 41 1 73 167 153 249 6.66e-31 115
Msa0313080 AT1G27370.3 55.670 97 41 1 73 167 153 249 6.66e-31 115
Msa0313080 AT1G27370.2 55.670 97 41 1 73 167 153 249 6.66e-31 115
Msa0313080 AT1G27370.4 55.670 97 41 1 73 167 153 249 6.66e-31 115
Msa0313080 AT1G27370.7 55.670 97 41 1 73 167 153 249 6.66e-31 115
Msa0313080 AT3G57920.1 56.731 104 40 1 64 167 34 132 4.67e-30 112
Msa0313080 AT1G27360.2 56.701 97 41 1 72 167 152 248 5.63e-30 113
Msa0313080 AT1G27360.1 56.701 97 41 1 72 167 152 248 5.63e-30 113
Msa0313080 AT1G27360.3 56.701 97 41 1 72 167 152 248 5.63e-30 113
Msa0313080 AT1G27360.4 56.701 97 41 1 72 167 152 248 5.63e-30 113
Msa0313080 AT5G43270.3 64.935 77 27 0 91 167 166 242 8.41e-30 113
Msa0313080 AT5G43270.1 64.935 77 27 0 91 167 166 242 8.41e-30 113
Msa0313080 AT5G43270.2 64.935 77 27 0 91 167 166 242 8.41e-30 113
Msa0313080 AT2G42200.1 64.474 76 27 0 92 167 72 147 1.84e-29 111
Msa0313080 AT5G50570.1 56.667 90 39 0 83 172 90 179 2.51e-29 110
Msa0313080 AT5G50570.2 56.667 90 39 0 83 172 90 179 2.51e-29 110
Msa0313080 AT5G50670.1 56.667 90 39 0 83 172 90 179 2.51e-29 110
Msa0313080 AT5G50670.2 56.667 90 39 0 83 172 90 179 2.51e-29 110
Msa0313080 AT1G02065.2 65.000 60 19 1 91 148 185 244 2.09e-20 85.1
Msa0313080 AT5G18830.2 49.383 81 41 0 87 167 131 211 3.88e-20 87.0
Msa0313080 AT5G18830.1 49.383 81 41 0 87 167 131 211 4.38e-20 87.0
Msa0313080 AT5G18830.3 49.383 81 41 0 87 167 131 211 4.70e-20 87.0
Msa0313080 AT2G47070.2 67.925 53 17 0 119 171 2 54 9.05e-18 80.1

Find 68 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATTTACCTTTACTTA+TGG 0.220696 2_4:-8346647 Msa0313080:CDS
ACAAAAGGGTGCTCATTTAT+TGG 0.224690 2_4:+8346786 None:intergenic
CACAAACCTTATGGCGCTTA+TGG 0.248219 2_4:+8346398 None:intergenic
ATATCCCTCAAATATATTAT+TGG 0.277920 2_4:+8344237 None:intergenic
AATGGTATTCACAAACCTTA+TGG 0.308780 2_4:+8346389 None:intergenic
AAGCCATGGTAATAAATTAA+TGG 0.327177 2_4:+8344265 None:intergenic
TTTACTTATGGATGGAAGTT+GGG 0.337434 2_4:-8346635 Msa0313080:CDS
ATGACTTGAAAAGGAGTTGT+AGG 0.355530 2_4:-8344582 Msa0313080:CDS
GGTTTGTGAATACCATTCTA+AGG 0.368542 2_4:-8346383 Msa0313080:CDS
TTGTCAACCTGCTACATTGC+TGG 0.372075 2_4:+8346320 None:intergenic
GAAGAGGAAGATGTGAGTTA+TGG 0.375825 2_4:-8346553 Msa0313080:CDS
CTTTACTTATGGATGGAAGT+TGG 0.392836 2_4:-8346636 Msa0313080:CDS
ATTCAAATTGATGTTATTTG+TGG 0.399031 2_4:-8344430 Msa0313080:three_prime_UTR
CTCACCAATAATATATTTGA+GGG 0.417935 2_4:-8344241 Msa0313080:three_prime_UTR
CAGAATTTGATGACTTGAAA+AGG 0.424158 2_4:-8344591 Msa0313080:CDS
AAGAGGGTGTGGACCACAAA+AGG 0.442762 2_4:+8346771 None:intergenic
TCTCACCAATAATATATTTG+AGG 0.442874 2_4:-8344242 Msa0313080:three_prime_UTR
GAGATGATGGGAAGAAGAAA+AGG 0.443829 2_4:-8346531 Msa0313080:CDS
AAGGAGTTGTAGGAGGCGTT+TGG 0.456087 2_4:-8344572 Msa0313080:CDS
TGAAGTAGAAGTAGAAGAAG+AGG 0.463169 2_4:-8346569 Msa0313080:CDS
ATGGATGGAAGTTGGGGTGA+GGG 0.465253 2_4:-8346628 Msa0313080:CDS
TTTATTGGTTGCCAACTTGA+GGG 0.465401 2_4:+8346801 None:intergenic
GATGTGAGTTATGGAGATGA+TGG 0.466595 2_4:-8346544 Msa0313080:CDS
AACAGGAAAGGGTCAAAAGC+TGG 0.479614 2_4:-8346487 Msa0313080:CDS
ATAAGGGTAAATAAATAGAA+TGG 0.480302 2_4:+8346745 None:intergenic
AGTTGTAGGAGGCGTTTGGC+TGG 0.487448 2_4:-8344568 Msa0313080:CDS
ATTTATTGGTTGCCAACTTG+AGG 0.488594 2_4:+8346800 None:intergenic
TAACATCAATTTGAATATGA+AGG 0.494767 2_4:+8344437 None:intergenic
CTTCTATACCAAGGGTGTTG+TGG 0.499867 2_4:-8346712 Msa0313080:CDS
TATGGATGGAAGTTGGGGTG+AGG 0.500828 2_4:-8346629 Msa0313080:CDS
TGACTGATCATCTTTACAAC+AGG 0.501584 2_4:-8346504 Msa0313080:CDS
GTTATTAAAGAATGAAAAGA+GGG 0.503992 2_4:+8346672 None:intergenic
CAACTTGAGGGTAACAGTGA+TGG 0.504518 2_4:+8346813 None:intergenic
TGAAAAGAGGGTGAAGTCAA+GGG 0.507320 2_4:+8346684 None:intergenic
ATGAAAAGAGGGTGAAGTCA+AGG 0.509681 2_4:+8346683 None:intergenic
ATCATCTTTACAACAGGAAA+GGG 0.510630 2_4:-8346498 Msa0313080:CDS
GATTTACCTTTACTTATGGA+TGG 0.521525 2_4:-8346643 Msa0313080:CDS
CAACTTCCATCCATAAGTAA+AGG 0.525127 2_4:+8346637 None:intergenic
TTGAAGCTCTGAAATGAGTA+CGG 0.530837 2_4:+8346354 None:intergenic
GCAGTACCATAAGCGCCATA+AGG 0.534642 2_4:-8346404 Msa0313080:CDS
TTACTTATGGATGGAAGTTG+GGG 0.539498 2_4:-8346634 Msa0313080:CDS
GGTAATAAATTAATGGTACA+AGG 0.560402 2_4:+8344272 None:intergenic
AGTTATTAAAGAATGAAAAG+AGG 0.561099 2_4:+8346671 None:intergenic
GCAGCAAACTTAGCATAAAG+AGG 0.566131 2_4:+8344373 None:intergenic
GGGGAAGACCACAACACCCT+TGG 0.567908 2_4:+8346704 None:intergenic
GATCATCTTTACAACAGGAA+AGG 0.569404 2_4:-8346499 Msa0313080:CDS
GAAAAGAGGGTGAAGTCAAG+GGG 0.570578 2_4:+8346685 None:intergenic
GTACGGAATGAGCCTTAGAA+TGG 0.572289 2_4:+8346371 None:intergenic
AGATGATGGGAAGAAGAAAA+GGG 0.572790 2_4:-8346530 Msa0313080:CDS
GTACCATTAATTTATTACCA+TGG 0.575456 2_4:-8344268 Msa0313080:three_prime_UTR
CAGTTATCAACTTGACAAGA+AGG 0.576877 2_4:+8346451 None:intergenic
ATTTGAATATGAAGGACTCA+AGG 0.580481 2_4:+8344445 None:intergenic
CACATACAATACAATTGCAA+AGG 0.586026 2_4:+8344307 None:intergenic
AACTTGAGGGTAACAGTGAT+GGG 0.587158 2_4:+8346814 None:intergenic
ACGTCTGAATATCACTGAGA+AGG 0.591085 2_4:-8344520 Msa0313080:exon
ATGTGAGTTATGGAGATGAT+GGG 0.595276 2_4:-8346543 Msa0313080:CDS
TCATTTCAGAGCTTCAACAA+AGG 0.599380 2_4:-8346348 Msa0313080:CDS
AGAGGGTGTGGACCACAAAA+GGG 0.600294 2_4:+8346772 None:intergenic
CATCACTGTTACCCTCAAGT+TGG 0.601011 2_4:-8346812 Msa0313080:five_prime_UTR
CAGCAAACTTAGCATAAAGA+GGG 0.607629 2_4:+8344374 None:intergenic
CTATATAGAGCATTAGCTGA+GGG 0.637733 2_4:+8346844 None:intergenic
ACTTGAAAAGGAGTTGTAGG+AGG 0.645868 2_4:-8344579 Msa0313080:CDS
TATTATTGGTGAGAAAGCCA+TGG 0.646118 2_4:+8344251 None:intergenic
CATAAGTAAAGGTAAATCTG+AGG 0.648136 2_4:+8346648 None:intergenic
TGATGGGAAGAAGAAAAGGG+TGG 0.655448 2_4:-8346527 Msa0313080:CDS
AGGAAAGGGTCAAAAGCTGG+AGG 0.666819 2_4:-8346484 Msa0313080:CDS
ACTATATAGAGCATTAGCTG+AGG 0.670283 2_4:+8346843 None:intergenic
GTTTGGCTGGACATAACGAG+AGG 0.681910 2_4:-8344555 Msa0313080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTCTATCAAATA+AGG - chr2_4:8344973-8344992 Msa0313080:intron 10.0%
!! ATATTAATATTTACTAGAAA+TGG + chr2_4:8345031-8345050 None:intergenic 10.0%
!!! TTATTTTTTAATATTCCAAT+AGG - chr2_4:8345155-8345174 Msa0313080:intron 10.0%
!!! AAAATGATAGATTTTTATAA+GGG + chr2_4:8345257-8345276 None:intergenic 10.0%
!!! TAAAATGATAGATTTTTATA+AGG + chr2_4:8345258-8345277 None:intergenic 10.0%
!!! TAAAAAAAACTTGTTTTATA+TGG + chr2_4:8345324-8345343 None:intergenic 10.0%
!!! AAAACAAGTTTTTTTTAATA+TGG - chr2_4:8345327-8345346 Msa0313080:intron 10.0%
!!! ATTTTGTAAAAATAAGATTA+AGG - chr2_4:8345408-8345427 Msa0313080:intron 10.0%
!!! TTTTGTAAAAATAAGATTAA+GGG - chr2_4:8345409-8345428 Msa0313080:intron 10.0%
!!! ATTTCATCATTATAATTTTT+TGG - chr2_4:8345495-8345514 Msa0313080:intron 10.0%
!!! TTTTTTATTGATAAATACCT+TGG - chr2_4:8344891-8344910 Msa0313080:intron 15.0%
!!! TCATTTTACTAAAAATAGTA+AGG - chr2_4:8345270-8345289 Msa0313080:intron 15.0%
!!! CATTTTACTAAAAATAGTAA+GGG - chr2_4:8345271-8345290 Msa0313080:intron 15.0%
!!! TTTATATGGACTTTTAAAAA+AGG + chr2_4:8345310-8345329 None:intergenic 15.0%
!! ATTAATTAGTTGTAAATCAT+GGG + chr2_4:8346391-8346410 None:intergenic 15.0%
!! AATTAATTAGTTGTAAATCA+TGG + chr2_4:8346392-8346411 None:intergenic 15.0%
!! TAATTATTAACTTATAGCTT+TGG - chr2_4:8346407-8346426 Msa0313080:CDS 15.0%
!! ATAAATAGAATGGAAAAAGA+GGG + chr2_4:8344301-8344320 None:intergenic 20.0%
!! AATAAATAGAATGGAAAAAG+AGG + chr2_4:8344302-8344321 None:intergenic 20.0%
!! ATAAGGGTAAATAAATAGAA+TGG + chr2_4:8344311-8344330 None:intergenic 20.0%
!! TAGAAGTAGAAAAAACATAA+GGG + chr2_4:8344327-8344346 None:intergenic 20.0%
!! ATAGAAGTAGAAAAAACATA+AGG + chr2_4:8344328-8344347 None:intergenic 20.0%
!! GTTATTAAAGAATGAAAAGA+GGG + chr2_4:8344384-8344403 None:intergenic 20.0%
!! AGTTATTAAAGAATGAAAAG+AGG + chr2_4:8344385-8344404 None:intergenic 20.0%
!! AAGATATTCAAGGAAAAAAA+AGG + chr2_4:8344771-8344790 None:intergenic 20.0%
!! TACTAATACTAAGATATTCA+AGG + chr2_4:8344781-8344800 None:intergenic 20.0%
!! AAATATTGAGGTTCAATATA+AGG + chr2_4:8344813-8344832 None:intergenic 20.0%
!! TTTGTGCATCATTATAAAAT+GGG - chr2_4:8345065-8345084 Msa0313080:intron 20.0%
!! TTTAAGCTTCTTAACAAATA+AGG - chr2_4:8345209-8345228 Msa0313080:intron 20.0%
!! TTAAGCTTCTTAACAAATAA+GGG - chr2_4:8345210-8345229 Msa0313080:intron 20.0%
!! TCAAAAAAAATGTTCAAATG+TGG - chr2_4:8345353-8345372 Msa0313080:intron 20.0%
!!! TTTTACTATAAAAGAGGTAA+TGG + chr2_4:8345380-8345399 None:intergenic 20.0%
!!! ACCTCTTTTATAGTAAAATA+TGG - chr2_4:8345382-8345401 Msa0313080:intron 20.0%
!!! ACCATATTTTACTATAAAAG+AGG + chr2_4:8345386-8345405 None:intergenic 20.0%
!! ATAAGATTAAGGGTAAAATA+CGG - chr2_4:8345419-8345438 Msa0313080:intron 20.0%
!! AAAAATAAAATAAAGGGAAG+TGG - chr2_4:8345452-8345471 Msa0313080:intron 20.0%
!! TGAACATCAATAAATATTCA+GGG + chr2_4:8345828-8345847 None:intergenic 20.0%
!! ATGAACATCAATAAATATTC+AGG + chr2_4:8345829-8345848 None:intergenic 20.0%
!!! CATGAAGTATCTTTAAAAAA+TGG + chr2_4:8346133-8346152 None:intergenic 20.0%
!!! CATTTTTTAAAGATACTTCA+TGG - chr2_4:8346131-8346150 Msa0313080:intron 20.0%
!! AAGACAGGAAAAAAAAATTA+GGG - chr2_4:8346158-8346177 Msa0313080:intron 20.0%
!! TAACATCAATTTGAATATGA+AGG + chr2_4:8346619-8346638 None:intergenic 20.0%
!!! ATTCAAATTGATGTTATTTG+TGG - chr2_4:8346623-8346642 Msa0313080:CDS 20.0%
!! ATATCCCTCAAATATATTAT+TGG + chr2_4:8346819-8346838 None:intergenic 20.0%
!!! TTTTTTCTACTTCTATACCA+AGG - chr2_4:8344332-8344351 Msa0313080:three_prime_UTR 25.0%
!! TTTTTCTACTTCTATACCAA+GGG - chr2_4:8344333-8344352 Msa0313080:three_prime_UTR 25.0%
! GGAAAAAAAAGGTGAAATTA+GGG + chr2_4:8344760-8344779 None:intergenic 25.0%
! AGGAAAAAAAAGGTGAAATT+AGG + chr2_4:8344761-8344780 None:intergenic 25.0%
!!! CACTTTTGAACATTTTTTAG+AGG + chr2_4:8345000-8345019 None:intergenic 25.0%
! CTTTGTGCATCATTATAAAA+TGG - chr2_4:8345064-8345083 Msa0313080:intron 25.0%
! ATGCTAACAATTGTATGTTA+AGG - chr2_4:8345090-8345109 Msa0313080:intron 25.0%
! GAAAATTGTAATCAACCTAT+TGG + chr2_4:8345173-8345192 None:intergenic 25.0%
!!! TAGGTTGATTACAATTTTCA+TGG - chr2_4:8345174-8345193 Msa0313080:intron 25.0%
! TTCTTAACAAATAAGGGTTA+TGG - chr2_4:8345216-8345235 Msa0313080:intron 25.0%
!!! TCATATTAGATGCACTTTTT+TGG - chr2_4:8345521-8345540 Msa0313080:intron 25.0%
!! ACCAACAAATTTTTCCAATT+GGG + chr2_4:8345783-8345802 None:intergenic 25.0%
!! TACCAACAAATTTTTCCAAT+TGG + chr2_4:8345784-8345803 None:intergenic 25.0%
! GAACATCAATAAATATTCAG+GGG + chr2_4:8345827-8345846 None:intergenic 25.0%
! ATATATGATCTTGAAGTTGA+AGG + chr2_4:8345973-8345992 None:intergenic 25.0%
!! CTTTAGTCATCACTTAATTT+TGG - chr2_4:8346055-8346074 Msa0313080:intron 25.0%
!!! TTTTTAAAGATACTTCATGG+AGG - chr2_4:8346134-8346153 Msa0313080:intron 25.0%
! CAAGACAGGAAAAAAAAATT+AGG - chr2_4:8346157-8346176 Msa0313080:intron 25.0%
!!! TTTTTTTTTGAAGAAGCACA+AGG + chr2_4:8346277-8346296 None:intergenic 25.0%
!! AGAACATTGTGTATATGTTT+TGG + chr2_4:8346336-8346355 None:intergenic 25.0%
! ACACAATGTTCTTCATATAT+AGG - chr2_4:8346344-8346363 Msa0313080:CDS 25.0%
!!! TAACTTATAGCTTTGGAATA+TGG - chr2_4:8346414-8346433 Msa0313080:CDS 25.0%
!! TTGGAATATGGTTTTTCTAT+AGG - chr2_4:8346426-8346445 Msa0313080:CDS 25.0%
! GGTAATAAATTAATGGTACA+AGG + chr2_4:8346784-8346803 None:intergenic 25.0%
! GTACCATTAATTTATTACCA+TGG - chr2_4:8346785-8346804 Msa0313080:five_prime_UTR 25.0%
! AAGCCATGGTAATAAATTAA+TGG + chr2_4:8346791-8346810 None:intergenic 25.0%
! TCTCACCAATAATATATTTG+AGG - chr2_4:8346811-8346830 Msa0313080:five_prime_UTR 25.0%
! CTCACCAATAATATATTTGA+GGG - chr2_4:8346812-8346831 Msa0313080:five_prime_UTR 25.0%
CATAAGTAAAGGTAAATCTG+AGG + chr2_4:8344408-8344427 None:intergenic 30.0%
CTCAGATTTACCTTTACTTA+TGG - chr2_4:8344406-8344425 Msa0313080:three_prime_UTR 30.0%
GATTTACCTTTACTTATGGA+TGG - chr2_4:8344410-8344429 Msa0313080:three_prime_UTR 30.0%
TTTACTTATGGATGGAAGTT+GGG - chr2_4:8344418-8344437 Msa0313080:three_prime_UTR 30.0%
ATCATCTTTACAACAGGAAA+GGG - chr2_4:8344555-8344574 Msa0313080:CDS 30.0%
AATGGTATTCACAAACCTTA+TGG + chr2_4:8344667-8344686 None:intergenic 30.0%
CTTAGTTATTAGTAAGACCA+AGG + chr2_4:8344911-8344930 None:intergenic 30.0%
TTTAAAAAAGGAGCCAAATG+TGG + chr2_4:8345298-8345317 None:intergenic 30.0%
!!! TTAGATGCACTTTTTTGGAT+AGG - chr2_4:8345526-8345545 Msa0313080:intron 30.0%
TTTGTAGGAGTGAAAATCAA+AGG + chr2_4:8345669-8345688 None:intergenic 30.0%
!! TTTGATTTTCACTCCTACAA+AGG - chr2_4:8345668-8345687 Msa0313080:intron 30.0%
AAGTTTAGCACTATATAGCT+AGG + chr2_4:8345704-8345723 None:intergenic 30.0%
!! TTGGAAAAATTTGTTGGTAC+AGG - chr2_4:8345785-8345804 Msa0313080:intron 30.0%
AAATGCTATTCGTCTATAGT+TGG + chr2_4:8346032-8346051 None:intergenic 30.0%
CAAATTTGTGACTTAAACGT+TGG - chr2_4:8346224-8346243 Msa0313080:intron 30.0%
TGGTTAATTTAGTATGCACA+AGG + chr2_4:8346316-8346335 None:intergenic 30.0%
! GTTTTTCTATAGGTTTCATG+AGG - chr2_4:8346436-8346455 Msa0313080:CDS 30.0%
! CAGAATTTGATGACTTGAAA+AGG - chr2_4:8346462-8346481 Msa0313080:CDS 30.0%
ATTTGAATATGAAGGACTCA+AGG + chr2_4:8346611-8346630 None:intergenic 30.0%
CACATACAATACAATTGCAA+AGG + chr2_4:8346749-8346768 None:intergenic 30.0%
! CTATATAGAGCATTAGCTGA+GGG + chr2_4:8344212-8344231 None:intergenic 35.0%
! ACTATATAGAGCATTAGCTG+AGG + chr2_4:8344213-8344232 None:intergenic 35.0%
! TTTATTGGTTGCCAACTTGA+GGG + chr2_4:8344255-8344274 None:intergenic 35.0%
! ATTTATTGGTTGCCAACTTG+AGG + chr2_4:8344256-8344275 None:intergenic 35.0%
!! ACAAAAGGGTGCTCATTTAT+TGG + chr2_4:8344270-8344289 None:intergenic 35.0%
! AATAAATGAGCACCCTTTTG+TGG - chr2_4:8344269-8344288 Msa0313080:three_prime_UTR 35.0%
CAACTTCCATCCATAAGTAA+AGG + chr2_4:8344419-8344438 None:intergenic 35.0%
CTTTACTTATGGATGGAAGT+TGG - chr2_4:8344417-8344436 Msa0313080:three_prime_UTR 35.0%
TTACTTATGGATGGAAGTTG+GGG - chr2_4:8344419-8344438 Msa0313080:three_prime_UTR 35.0%
TGAAGTAGAAGTAGAAGAAG+AGG - chr2_4:8344484-8344503 Msa0313080:three_prime_UTR 35.0%
ATGTGAGTTATGGAGATGAT+GGG - chr2_4:8344510-8344529 Msa0313080:exon 35.0%
!! AGATGATGGGAAGAAGAAAA+GGG - chr2_4:8344523-8344542 Msa0313080:exon 35.0%
TGACTGATCATCTTTACAAC+AGG - chr2_4:8344549-8344568 Msa0313080:CDS 35.0%
GATCATCTTTACAACAGGAA+AGG - chr2_4:8344554-8344573 Msa0313080:CDS 35.0%
CAGTTATCAACTTGACAAGA+AGG + chr2_4:8344605-8344624 None:intergenic 35.0%
GGTTTGTGAATACCATTCTA+AGG - chr2_4:8344670-8344689 Msa0313080:intron 35.0%
!! TTGAAGCTCTGAAATGAGTA+CGG + chr2_4:8344702-8344721 None:intergenic 35.0%
TCATTTCAGAGCTTCAACAA+AGG - chr2_4:8344705-8344724 Msa0313080:intron 35.0%
CCACTTCACATCAAATATTG+AGG + chr2_4:8344825-8344844 None:intergenic 35.0%
CCTCAATATTTGATGTGAAG+TGG - chr2_4:8344822-8344841 Msa0313080:intron 35.0%
AAATAGTAAGGGTCCACATT+TGG - chr2_4:8345282-8345301 Msa0313080:intron 35.0%
GGAAAATGTCTGTAAGTTAC+TGG - chr2_4:8345547-8345566 Msa0313080:intron 35.0%
AGGCTAACTATATCCTTTGT+AGG + chr2_4:8345684-8345703 None:intergenic 35.0%
GCCCAATTGGAAAAATTTGT+TGG - chr2_4:8345779-8345798 Msa0313080:intron 35.0%
! TATAGCAGTGTTACTGTGAT+TGG - chr2_4:8345992-8346011 Msa0313080:intron 35.0%
! TTTTGTGTTGTCAACTTCGT+GGG + chr2_4:8346097-8346116 None:intergenic 35.0%
GACACAGGTTAAAACTGATT+TGG + chr2_4:8346255-8346274 None:intergenic 35.0%
ATGACTTGAAAAGGAGTTGT+AGG - chr2_4:8346471-8346490 Msa0313080:CDS 35.0%
CAGCAAACTTAGCATAAAGA+GGG + chr2_4:8346682-8346701 None:intergenic 35.0%
TATTATTGGTGAGAAAGCCA+TGG + chr2_4:8346805-8346824 None:intergenic 35.0%
AACTTGAGGGTAACAGTGAT+GGG + chr2_4:8344242-8344261 None:intergenic 40.0%
TAGAATGGAAAAAGAGGGTG+TGG + chr2_4:8344296-8344315 None:intergenic 40.0%
TGAAAAGAGGGTGAAGTCAA+GGG + chr2_4:8344372-8344391 None:intergenic 40.0%
ATGAAAAGAGGGTGAAGTCA+AGG + chr2_4:8344373-8344392 None:intergenic 40.0%
GAAGAGGAAGATGTGAGTTA+TGG - chr2_4:8344500-8344519 Msa0313080:three_prime_UTR 40.0%
GATGTGAGTTATGGAGATGA+TGG - chr2_4:8344509-8344528 Msa0313080:exon 40.0%
!! GAGATGATGGGAAGAAGAAA+AGG - chr2_4:8344522-8344541 Msa0313080:exon 40.0%
TATTTGATGTGAAGTGGCTG+AGG - chr2_4:8344828-8344847 Msa0313080:intron 40.0%
ATTTGATGTGAAGTGGCTGA+GGG - chr2_4:8344829-8344848 Msa0313080:intron 40.0%
CTATGAGTTGTCTCTTGCTA+GGG + chr2_4:8344934-8344953 None:intergenic 40.0%
GATTTAGCAACTTGCCCAAT+TGG - chr2_4:8345766-8345785 Msa0313080:intron 40.0%
TACATAGAACTCACACTCAC+AGG - chr2_4:8345863-8345882 Msa0313080:intron 40.0%
ACATAGAACTCACACTCACA+GGG - chr2_4:8345864-8345883 Msa0313080:intron 40.0%
TTTGTGTTGTCAACTTCGTG+GGG + chr2_4:8346096-8346115 None:intergenic 40.0%
! CTTTTGTGTTGTCAACTTCG+TGG + chr2_4:8346098-8346117 None:intergenic 40.0%
CACGAAGTTGACAACACAAA+AGG - chr2_4:8346096-8346115 Msa0313080:intron 40.0%
TTTAGTATGCACAAGGACAC+AGG + chr2_4:8346309-8346328 None:intergenic 40.0%
ACTTGAAAAGGAGTTGTAGG+AGG - chr2_4:8346474-8346493 Msa0313080:CDS 40.0%
! GATATTCAGACGTGCTTTTG+CGG + chr2_4:8346526-8346545 None:intergenic 40.0%
ACGTCTGAATATCACTGAGA+AGG - chr2_4:8346533-8346552 Msa0313080:CDS 40.0%
GCAGCAAACTTAGCATAAAG+AGG + chr2_4:8346683-8346702 None:intergenic 40.0%
CAACTTGAGGGTAACAGTGA+TGG + chr2_4:8344243-8344262 None:intergenic 45.0%
CATCACTGTTACCCTCAAGT+TGG - chr2_4:8344241-8344260 Msa0313080:three_prime_UTR 45.0%
!! CTTCTATACCAAGGGTGTTG+TGG - chr2_4:8344341-8344360 Msa0313080:three_prime_UTR 45.0%
GAAAAGAGGGTGAAGTCAAG+GGG + chr2_4:8344371-8344390 None:intergenic 45.0%
TGATGGGAAGAAGAAAAGGG+TGG - chr2_4:8344526-8344545 Msa0313080:CDS 45.0%
AACAGGAAAGGGTCAAAAGC+TGG - chr2_4:8344566-8344585 Msa0313080:CDS 45.0%
CACAAACCTTATGGCGCTTA+TGG + chr2_4:8344658-8344677 None:intergenic 45.0%
GTACGGAATGAGCCTTAGAA+TGG + chr2_4:8344685-8344704 None:intergenic 45.0%
TTGTCAACCTGCTACATTGC+TGG + chr2_4:8344736-8344755 None:intergenic 45.0%
CCTATGAGTTGTCTCTTGCT+AGG + chr2_4:8344935-8344954 None:intergenic 45.0%
CCTAGCAAGAGACAACTCAT+AGG - chr2_4:8344932-8344951 Msa0313080:intron 45.0%
TTACCCCAACATGCTGAGAT+AGG - chr2_4:8345929-8345948 Msa0313080:intron 45.0%
TACCCCAACATGCTGAGATA+GGG - chr2_4:8345930-8345949 Msa0313080:intron 45.0%
TGTCCCTATCTCAGCATGTT+GGG + chr2_4:8345936-8345955 None:intergenic 45.0%
ATGTCCCTATCTCAGCATGT+TGG + chr2_4:8345937-8345956 None:intergenic 45.0%
ATACTTCATGGAGGCAAGAC+AGG - chr2_4:8346143-8346162 Msa0313080:intron 45.0%
! TTGAAGAAGCACAAGGACAC+AGG + chr2_4:8346270-8346289 None:intergenic 45.0%
!!! ATTTTGTAAAAATAAAATAA+AGG - chr2_4:8345445-8345464 Msa0313080:intron 5.0%
!!! TTTTGTAAAAATAAAATAAA+GGG - chr2_4:8345446-8345465 Msa0313080:intron 5.0%
! AGAGGGTGTGGACCACAAAA+GGG + chr2_4:8344284-8344303 None:intergenic 50.0%
! AAGAGGGTGTGGACCACAAA+AGG + chr2_4:8344285-8344304 None:intergenic 50.0%
TATGGATGGAAGTTGGGGTG+AGG - chr2_4:8344424-8344443 Msa0313080:three_prime_UTR 50.0%
ATGGATGGAAGTTGGGGTGA+GGG - chr2_4:8344425-8344444 Msa0313080:three_prime_UTR 50.0%
AGGAAAGGGTCAAAAGCTGG+AGG - chr2_4:8344569-8344588 Msa0313080:CDS 50.0%
GCAGTACCATAAGCGCCATA+AGG - chr2_4:8344649-8344668 Msa0313080:intron 50.0%
! GGTTTTGCCAGCAATGTAGC+AGG - chr2_4:8344726-8344745 Msa0313080:intron 50.0%
GTCCCTATCTCAGCATGTTG+GGG + chr2_4:8345935-8345954 None:intergenic 50.0%
!! AAGGAGTTGTAGGAGGCGTT+TGG - chr2_4:8346481-8346500 Msa0313080:CDS 50.0%
GTTTGGCTGGACATAACGAG+AGG - chr2_4:8346498-8346517 Msa0313080:CDS 50.0%
!! AGTTGTAGGAGGCGTTTGGC+TGG - chr2_4:8346485-8346504 Msa0313080:CDS 55.0%
GGGGAAGACCACAACACCCT+TGG + chr2_4:8344352-8344371 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 8344202 8346873 8344202 ID=Msa0313080;Name=Msa0313080
chr2_4 mRNA 8344202 8346873 8344202 ID=Msa0313080-mRNA-1;Parent=Msa0313080;Name=Msa0313080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=127|1|1|1|1|1|2|323|173
chr2_4 exon 8344202 8344627 8344202 ID=Msa0313080-mRNA-1:exon:3225;Parent=Msa0313080-mRNA-1
chr2_4 exon 8346328 8346873 8346328 ID=Msa0313080-mRNA-1:exon:3224;Parent=Msa0313080-mRNA-1
chr2_4 five_prime_UTR 8346747 8346873 8346747 ID=Msa0313080-mRNA-1:five_prime_utr;Parent=Msa0313080-mRNA-1
chr2_4 CDS 8346328 8346746 8346328 ID=Msa0313080-mRNA-1:cds;Parent=Msa0313080-mRNA-1
chr2_4 CDS 8344525 8344627 8344525 ID=Msa0313080-mRNA-1:cds;Parent=Msa0313080-mRNA-1
chr2_4 three_prime_UTR 8344202 8344524 8344202 ID=Msa0313080-mRNA-1:three_prime_utr;Parent=Msa0313080-mRNA-1
Gene Sequence

>Msa0313080

ATGTTTTTTCTACTTCTATACCAAGGGTGTTGTGGTCTTCCCCTTGACTTCACCCTCTTTTCATTCTTTAATAACTCCTCAGATTTACCTTTACTTATGGATGGAAGTTGGGGTGAGGGAAAGAGAATCTATGAGTACAGAGAAGAAGATGAGTATGAAGTAGAAGTAGAAGAAGAGGAAGATGTGAGTTATGGAGATGATGGGAAGAAGAAAAGGGTGGTGACTGATCATCTTTACAACAGGAAAGGGTCAAAAGCTGGAGGATCAGTGACACCTTCTTGTCAAGTTGATAACTGTAATGCTGATCTAAGTGTTGCTAAGCAGTACCATAAGCGCCATAAGGTTTGTGAATACCATTCTAAGGCTCATTCCGTACTCATTTCAGAGCTTCAACAAAGGTTTTGCCAGCAATGTAGCAGGTTTCATGAGGTATCAGAATTTGATGACTTGAAAAGGAGTTGTAGGAGGCGTTTGGCTGGACATAACGAGAGGCGCCGCAAAAGCACGTCTGAATATCACTGA
Protein sequence

>Msa0313080

MFFLLLYQGCCGLPLDFTLFSFFNNSSDLPLLMDGSWGEGKRIYEYREEDEYEVEVEEEEDVSYGDDGKKKRVVTDHLYNRKGSKAGGSVTPSCQVDNCNADLSVAKQYHKRHKVCEYHSKAHSVLISELQQRFCQQCSRFHEVSEFDDLKRSCRRRLAGHNERRRKSTSEYH*