AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0301280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0301280 MtrunA17_Chr2g0282521 91.608 143 10 1 1 141 1 143 2.56e-79 230
Msa0301280 MtrunA17_Chr4g0046991 64.085 142 48 2 1 140 11 151 9.95e-52 161
Msa0301280 MtrunA17_Chr8g0360361 66.667 81 27 0 59 139 62 142 1.53e-36 123
Msa0301280 MtrunA17_Chr7g0231911 70.238 84 25 0 54 137 141 224 1.20e-35 125
Msa0301280 MtrunA17_Chr8g0334671 67.470 83 27 0 54 136 179 261 3.88e-35 124
Msa0301280 MtrunA17_Chr5g0420051 61.053 95 37 0 46 140 165 259 5.62e-35 125
Msa0301280 MtrunA17_Chr1g0162871 56.410 117 40 3 23 136 70 178 1.10e-34 127
Msa0301280 MtrunA17_Chr7g0258471 67.059 85 24 1 55 135 131 215 1.55e-33 121
Msa0301280 MtrunA17_Chr2g0302791 69.333 75 23 0 62 136 139 213 2.65e-33 123
Msa0301280 MtrunA17_Chr4g0061651 70.886 79 23 0 57 135 118 196 7.93e-33 120
Msa0301280 MtrunA17_Chr7g0270541 57.692 104 34 1 33 136 126 219 1.08e-32 121
Msa0301280 MtrunA17_Chr3g0122411 56.842 95 40 1 46 139 148 242 1.23e-32 119
Msa0301280 MtrunA17_Chr7g0224821 65.432 81 28 0 56 136 71 151 2.07e-32 117
Msa0301280 MtrunA17_Chr2g0308321 67.500 80 26 0 62 141 171 250 3.95e-32 119
Msa0301280 MtrunA17_Chr1g0194121 61.957 92 34 1 49 139 133 224 1.09e-31 118
Msa0301280 MtrunA17_Chr3g0132091 42.568 148 70 2 7 139 3 150 8.13e-31 113
Msa0301280 MtrunA17_Chr8g0386271 61.905 84 32 0 56 139 93 176 8.58e-29 108
Msa0301280 MtrunA17_Chr8g0377421 42.857 133 58 5 8 135 86 205 2.11e-25 97.8
Msa0301280 MtrunA17_Chr8g0377401 57.895 76 32 0 59 134 159 234 3.13e-25 96.7
Msa0301280 MtrunA17_Chr2g0317041 55.000 60 27 0 49 108 86 145 3.59e-19 78.6
Msa0301280 MtrunA17_Chr2g0286661 50.000 74 37 0 62 135 103 176 7.73e-19 81.6
Msa0301280 MtrunA17_Chr1g0175031 60.714 56 22 0 53 108 65 120 9.02e-17 71.6
Msa0301280 MtrunA17_Chr8g0377431 48.214 56 29 0 60 115 149 204 6.69e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0301280 AT2G33810.1 70.000 90 24 1 53 139 42 131 2.50e-39 129
Msa0301280 AT1G53160.2 62.626 99 37 0 41 139 33 131 2.74e-38 128
Msa0301280 AT1G53160.1 62.626 99 37 0 41 139 33 131 2.74e-38 128
Msa0301280 AT1G53160.3 62.626 99 37 0 41 139 33 131 2.87e-38 128
Msa0301280 AT1G20980.1 68.539 89 26 1 49 137 109 195 7.14e-36 130
Msa0301280 AT1G76580.1 68.750 80 25 0 60 139 112 191 1.89e-34 126
Msa0301280 AT3G60030.1 63.830 94 29 2 50 139 112 204 4.39e-34 125
Msa0301280 AT1G02065.1 68.293 82 26 0 54 135 180 261 4.56e-34 121
Msa0301280 AT1G76580.3 68.750 80 25 0 60 139 112 191 8.65e-34 124
Msa0301280 AT3G15270.1 65.823 79 25 1 59 135 58 136 1.03e-33 116
Msa0301280 AT1G76580.2 68.750 80 25 0 60 139 112 191 1.09e-33 124
Msa0301280 AT2G47070.1 60.825 97 34 1 43 139 91 183 1.33e-33 124
Msa0301280 AT1G69170.1 70.886 79 23 0 57 135 119 197 4.73e-33 120
Msa0301280 AT1G27370.6 55.670 97 41 1 41 135 153 249 1.81e-31 115
Msa0301280 AT1G27370.1 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0301280 AT1G27370.5 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0301280 AT1G27370.3 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0301280 AT1G27370.2 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0301280 AT1G27370.4 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0301280 AT1G27370.7 55.670 97 41 1 41 135 153 249 4.11e-31 115
Msa0301280 AT1G27360.2 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0301280 AT1G27360.1 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0301280 AT1G27360.3 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0301280 AT1G27360.4 56.701 97 41 1 40 135 152 248 1.91e-30 113
Msa0301280 AT3G57920.1 56.731 104 40 1 32 135 34 132 2.12e-30 112
Msa0301280 AT5G43270.3 64.103 78 28 0 59 136 166 243 3.48e-30 112
Msa0301280 AT5G43270.1 64.103 78 28 0 59 136 166 243 3.48e-30 112
Msa0301280 AT5G43270.2 64.103 78 28 0 59 136 166 243 3.48e-30 112
Msa0301280 AT2G42200.1 63.636 77 28 0 60 136 72 148 4.42e-30 112
Msa0301280 AT5G50570.1 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0301280 AT5G50570.2 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0301280 AT5G50670.1 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0301280 AT5G50670.2 56.667 90 39 0 51 140 90 179 1.63e-29 110
Msa0301280 AT1G02065.2 61.538 65 23 1 54 116 180 244 7.11e-21 85.1
Msa0301280 AT5G18830.2 49.383 81 41 0 55 135 131 211 3.35e-20 85.9
Msa0301280 AT5G18830.1 49.383 81 41 0 55 135 131 211 3.88e-20 85.9
Msa0301280 AT5G18830.3 49.383 81 41 0 55 135 131 211 4.29e-20 85.5
Msa0301280 AT2G47070.2 67.925 53 17 0 87 139 2 54 3.86e-19 82.8

Find 71 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATTTACCTTAACTTA+TGG 0.220696 2_3:-57123377 Msa0301280:exon
ACAAAAGGGTGCTCATTTAT+TGG 0.224690 2_3:+57123516 None:intergenic
CACAAACCTTATGGCGCTTA+TGG 0.248219 2_3:+57123128 None:intergenic
ATATCCCTCAAATATATTAT+TGG 0.277920 2_3:+57120960 None:intergenic
AATGGTATTCACAAACCTTA+TGG 0.308780 2_3:+57123119 None:intergenic
GAAGAGGAAGATGTGAGTTA+TGG 0.319891 2_3:-57123283 Msa0301280:CDS
AAGCCATGGTAATAAATTAA+TGG 0.327177 2_3:+57120988 None:intergenic
AAGAGGAAGATGTGAGTTAT+GGG 0.331465 2_3:-57123282 Msa0301280:CDS
ATGACTTGAAAAGGAGTTGT+AGG 0.355530 2_3:-57121305 Msa0301280:CDS
TTAACTTATGGATGGAAGTT+GGG 0.358056 2_3:-57123365 Msa0301280:exon
GGTTTGTGAATACCATTCTA+AGG 0.368542 2_3:-57123113 Msa0301280:CDS
TTGTCAACCTGCTACATTGC+TGG 0.372075 2_3:+57123050 None:intergenic
CTTAACTTATGGATGGAAGT+TGG 0.386744 2_3:-57123366 Msa0301280:exon
ATTCAAATTGATGTTATTTG+TGG 0.399031 2_3:-57121153 Msa0301280:three_prime_UTR
CTCACCAATAATATATTTGA+GGG 0.417935 2_3:-57120964 Msa0301280:three_prime_UTR
CAGAATTTGATGACTTGAAA+AGG 0.424158 2_3:-57121314 Msa0301280:CDS
CAACTTCCATCCATAAGTTA+AGG 0.431263 2_3:+57123367 None:intergenic
AAGAGGGTGTGGACCACAAA+AGG 0.442762 2_3:+57123501 None:intergenic
TCTCACCAATAATATATTTG+AGG 0.442874 2_3:-57120965 Msa0301280:three_prime_UTR
GGGATGATGGGAAGAAGAAA+AGG 0.444953 2_3:-57123261 Msa0301280:CDS
AAGGAGTTGTAGGAGGCGTT+TGG 0.456087 2_3:-57121295 Msa0301280:CDS
TGAAGTAGAAGTAGAAGAAG+AGG 0.463169 2_3:-57123299 Msa0301280:CDS
ATGGATGGAAGTTGGGGTGA+GGG 0.465253 2_3:-57123358 Msa0301280:CDS
TTTATTGGTTGCCAACTTGA+GGG 0.465401 2_3:+57123531 None:intergenic
GATGTGAGTTATGGGGATGA+TGG 0.475010 2_3:-57123274 Msa0301280:CDS
AACAGGAAAGGGTCAAAAGC+TGG 0.479614 2_3:-57123217 Msa0301280:CDS
CTTCTATACCAAGGTTGTTG+TGG 0.480026 2_3:-57123442 Msa0301280:five_prime_UTR
ATAAGGGTAAATAAATAGAA+TGG 0.480302 2_3:+57123475 None:intergenic
AGTTGTAGGAGGCGTTTGGC+TGG 0.487448 2_3:-57121291 Msa0301280:CDS
ATTTATTGGTTGCCAACTTG+AGG 0.488594 2_3:+57123530 None:intergenic
TAACATCAATTTGAATATGA+AGG 0.494767 2_3:+57121160 None:intergenic
TATGGATGGAAGTTGGGGTG+AGG 0.500828 2_3:-57123359 Msa0301280:CDS
TGACTGATCATCTTTACAAC+AGG 0.501584 2_3:-57123234 Msa0301280:CDS
GTTATTAAAGAATGAAAAGA+GGG 0.503992 2_3:+57123402 None:intergenic
CAACTTGAGGGTAACAGTGA+TGG 0.504518 2_3:+57123543 None:intergenic
TGAAAAGAGGGTGAAGTCAA+GGG 0.507320 2_3:+57123414 None:intergenic
ATGAAAAGAGGGTGAAGTCA+AGG 0.509681 2_3:+57123413 None:intergenic
ATCATCTTTACAACAGGAAA+GGG 0.510630 2_3:-57123228 Msa0301280:CDS
TTGAAGCTCTGAAATGAGTA+CGG 0.530837 2_3:+57123084 None:intergenic
GCAGTACCATAAGCGCCATA+AGG 0.534642 2_3:-57123134 Msa0301280:CDS
AGAGGAAGATGTGAGTTATG+GGG 0.544242 2_3:-57123281 Msa0301280:CDS
GATTTACCTTAACTTATGGA+TGG 0.546093 2_3:-57123373 Msa0301280:exon
ATGTGAGTTATGGGGATGAT+GGG 0.550503 2_3:-57123273 Msa0301280:CDS
GGATGATGGGAAGAAGAAAA+GGG 0.556520 2_3:-57123260 Msa0301280:CDS
GGTAATAAATTAATGGTACA+AGG 0.560402 2_3:+57120995 None:intergenic
AGTTATTAAAGAATGAAAAG+AGG 0.561099 2_3:+57123401 None:intergenic
GCAGCAAACTTAGCATAAAG+AGG 0.566131 2_3:+57121096 None:intergenic
TAACTTATGGATGGAAGTTG+GGG 0.568515 2_3:-57123364 Msa0301280:exon
GATCATCTTTACAACAGGAA+AGG 0.569404 2_3:-57123229 Msa0301280:CDS
GAAAAGAGGGTGAAGTCAAG+GGG 0.570578 2_3:+57123415 None:intergenic
GTACGGAATGAGCCTTAGAA+TGG 0.572289 2_3:+57123101 None:intergenic
GTACCATTAATTTATTACCA+TGG 0.575456 2_3:-57120991 Msa0301280:three_prime_UTR
CAGTTATCAACTTGACAAGA+AGG 0.576877 2_3:+57123181 None:intergenic
ATTTGAATATGAAGGACTCA+AGG 0.580481 2_3:+57121168 None:intergenic
CACATACAATACAATTGCAA+AGG 0.586026 2_3:+57121030 None:intergenic
AACTTGAGGGTAACAGTGAT+GGG 0.587158 2_3:+57123544 None:intergenic
ATAGAGCATTAGCTGAGGGC+CGG 0.588404 2_3:+57123578 None:intergenic
ACGTCTGAATATCACTGAGA+AGG 0.591085 2_3:-57121243 Msa0301280:exon
TCATTTCAGAGCTTCAACAA+AGG 0.599380 2_3:-57123078 Msa0301280:CDS
AGAGGGTGTGGACCACAAAA+GGG 0.600294 2_3:+57123502 None:intergenic
GGGGAAGACCACAACAACCT+TGG 0.600711 2_3:+57123434 None:intergenic
CATCACTGTTACCCTCAAGT+TGG 0.601011 2_3:-57123542 Msa0301280:five_prime_UTR
CAGCAAACTTAGCATAAAGA+GGG 0.607629 2_3:+57121097 None:intergenic
ACTTGAAAAGGAGTTGTAGG+AGG 0.645868 2_3:-57121302 Msa0301280:CDS
CTATATAGAGCATTAGCTGA+GGG 0.646069 2_3:+57123574 None:intergenic
TATTATTGGTGAGAAAGCCA+TGG 0.646118 2_3:+57120974 None:intergenic
TGATGGGAAGAAGAAAAGGG+TGG 0.662923 2_3:-57123257 Msa0301280:CDS
AGGAAAGGGTCAAAAGCTGG+AGG 0.666819 2_3:-57123214 Msa0301280:CDS
ACTATATAGAGCATTAGCTG+AGG 0.670283 2_3:+57123573 None:intergenic
CATAAGTTAAGGTAAATCTG+AGG 0.674591 2_3:+57123378 None:intergenic
GTTTGGCTGGACATAACGAG+AGG 0.681910 2_3:-57121278 Msa0301280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTCTATCAAATA+AGG - chr2_3:57121701-57121720 Msa0301280:intron 10.0%
!! ATATTAATATTTACTAGAAA+TGG + chr2_3:57121759-57121778 None:intergenic 10.0%
!!! TTATTTTTTAATATTCCAAT+AGG - chr2_3:57121883-57121902 Msa0301280:intron 10.0%
!!! AAAATGATAGATTTTTATAA+GGG + chr2_3:57121985-57122004 None:intergenic 10.0%
!!! TAAAATGATAGATTTTTATA+AGG + chr2_3:57121986-57122005 None:intergenic 10.0%
!!! AAAAAAAAACTTGTTTTATA+TGG + chr2_3:57122052-57122071 None:intergenic 10.0%
!!! ATTTTGTAAAAATAAGATTA+AGG - chr2_3:57122137-57122156 Msa0301280:intron 10.0%
!!! TTTTGTAAAAATAAGATTAA+GGG - chr2_3:57122138-57122157 Msa0301280:intron 10.0%
!!! ATTTCATCATTATAATTTTT+TGG - chr2_3:57122223-57122242 Msa0301280:intron 10.0%
!!! TCATTTTACTAAAAATAGTA+AGG - chr2_3:57121998-57122017 Msa0301280:intron 15.0%
!!! CATTTTACTAAAAATAGTAA+GGG - chr2_3:57121999-57122018 Msa0301280:intron 15.0%
!!! TTTATATGGACTTTTAAAAA+AGG + chr2_3:57122038-57122057 None:intergenic 15.0%
!! ATTAATTAGTTGTAAATCAT+GGG + chr2_3:57123121-57123140 None:intergenic 15.0%
!! AATTAATTAGTTGTAAATCA+TGG + chr2_3:57123122-57123141 None:intergenic 15.0%
!! TAATTATTAACTTATAGCTT+TGG - chr2_3:57123137-57123156 Msa0301280:CDS 15.0%
!! ATAAATAGAATGGAAAAAGA+GGG + chr2_3:57121024-57121043 None:intergenic 20.0%
!! AATAAATAGAATGGAAAAAG+AGG + chr2_3:57121025-57121044 None:intergenic 20.0%
!! ATAAGGGTAAATAAATAGAA+TGG + chr2_3:57121034-57121053 None:intergenic 20.0%
!! TAGAAGTAGAAAAAACATAA+GGG + chr2_3:57121050-57121069 None:intergenic 20.0%
!! ATAGAAGTAGAAAAAACATA+AGG + chr2_3:57121051-57121070 None:intergenic 20.0%
!! GTTATTAAAGAATGAAAAGA+GGG + chr2_3:57121107-57121126 None:intergenic 20.0%
!! AGTTATTAAAGAATGAAAAG+AGG + chr2_3:57121108-57121127 None:intergenic 20.0%
!! TACTAATACTAAGATATTCA+AGG + chr2_3:57121505-57121524 None:intergenic 20.0%
!! AAATATTGAGGTTCAATATA+AGG + chr2_3:57121537-57121556 None:intergenic 20.0%
!!! CTTTTTATTGATAAATACCT+TGG - chr2_3:57121615-57121634 Msa0301280:intron 20.0%
!! TTTGTGCATCATTATAAAAT+GGG - chr2_3:57121793-57121812 Msa0301280:intron 20.0%
!! TTTAAGCTTCTTAACAAATA+AGG - chr2_3:57121937-57121956 Msa0301280:intron 20.0%
!! TTAAGCTTCTTAACAAATAA+GGG - chr2_3:57121938-57121957 Msa0301280:intron 20.0%
!! TCAAAAAAAATGTTCAAATG+TGG - chr2_3:57122082-57122101 Msa0301280:intron 20.0%
!!! TTTTACTATAAAAGAGGTAA+TGG + chr2_3:57122109-57122128 None:intergenic 20.0%
!!! ACCTCTTTTATAGTAAAATA+TGG - chr2_3:57122111-57122130 Msa0301280:intron 20.0%
!!! ACCATATTTTACTATAAAAG+AGG + chr2_3:57122115-57122134 None:intergenic 20.0%
!! ATAAGATTAAGGGTAAAATA+CGG - chr2_3:57122148-57122167 Msa0301280:intron 20.0%
!! TAAAAATAAATAAAGGGAAG+TGG - chr2_3:57122180-57122199 Msa0301280:intron 20.0%
!!! CATGAAGTATCTTTAAAAAA+TGG + chr2_3:57122864-57122883 None:intergenic 20.0%
!!! CATTTTTTAAAGATACTTCA+TGG - chr2_3:57122862-57122881 Msa0301280:intron 20.0%
!!! TTTTTTTTTGAAGAAGCATA+AGG + chr2_3:57123010-57123029 None:intergenic 20.0%
!! TAACATCAATTTGAATATGA+AGG + chr2_3:57123349-57123368 None:intergenic 20.0%
!!! ATTCAAATTGATGTTATTTG+TGG - chr2_3:57123353-57123372 Msa0301280:CDS 20.0%
!! ATATCCCTCAAATATATTAT+TGG + chr2_3:57123549-57123568 None:intergenic 20.0%
!!! TTTTTTCTACTTCTATACCA+AGG - chr2_3:57121055-57121074 Msa0301280:three_prime_UTR 25.0%
! GAAAAAAAGAGGTGAAATTA+GGG + chr2_3:57121483-57121502 None:intergenic 25.0%
! AGATATTCAAGGAAAAAAAG+AGG + chr2_3:57121494-57121513 None:intergenic 25.0%
!!! CACTTTTGAACATTTTTTAG+AGG + chr2_3:57121728-57121747 None:intergenic 25.0%
! CTTTGTGCATCATTATAAAA+TGG - chr2_3:57121792-57121811 Msa0301280:intron 25.0%
! ATGCTAACAATTGTATGTTA+AGG - chr2_3:57121818-57121837 Msa0301280:intron 25.0%
! GAAAATTGTAATCAACCTAT+TGG + chr2_3:57121901-57121920 None:intergenic 25.0%
!!! TAGGTTGATTACAATTTTCA+TGG - chr2_3:57121902-57121921 Msa0301280:intron 25.0%
! TTCTTAACAAATAAGGGTTA+TGG - chr2_3:57121944-57121963 Msa0301280:intron 25.0%
!!! TCATATTAGATGCACTTTTT+TGG - chr2_3:57122249-57122268 Msa0301280:intron 25.0%
!! ACCAACAAATTTTTCCAATT+GGG + chr2_3:57122514-57122533 None:intergenic 25.0%
!! TACCAACAAATTTTTCCAAT+TGG + chr2_3:57122515-57122534 None:intergenic 25.0%
! TGAACATCAATACATATTCA+GGG + chr2_3:57122559-57122578 None:intergenic 25.0%
! ATGAACATCAATACATATTC+AGG + chr2_3:57122560-57122579 None:intergenic 25.0%
! ATATATGATCTTGAAGTTGA+AGG + chr2_3:57122702-57122721 None:intergenic 25.0%
!! CTTTAGTCATCACTTAATTT+TGG - chr2_3:57122786-57122805 Msa0301280:intron 25.0%
!!! TTTTTAAAGATACTTCATGG+AGG - chr2_3:57122865-57122884 Msa0301280:intron 25.0%
! GACAGGAAGAAAAAAAATTA+AGG - chr2_3:57122891-57122910 Msa0301280:intron 25.0%
!! AGAACATTGTGTATATGTTT+TGG + chr2_3:57123066-57123085 None:intergenic 25.0%
! ACACAATGTTCTTCATATAT+AGG - chr2_3:57123074-57123093 Msa0301280:CDS 25.0%
!!! TAACTTATAGCTTTGGAATA+TGG - chr2_3:57123144-57123163 Msa0301280:CDS 25.0%
!! TTGGAATATGGTTTTTCTAT+AGG - chr2_3:57123156-57123175 Msa0301280:CDS 25.0%
! GGTAATAAATTAATGGTACA+AGG + chr2_3:57123514-57123533 None:intergenic 25.0%
! GTACCATTAATTTATTACCA+TGG - chr2_3:57123515-57123534 Msa0301280:five_prime_UTR 25.0%
! AAGCCATGGTAATAAATTAA+TGG + chr2_3:57123521-57123540 None:intergenic 25.0%
! TCTCACCAATAATATATTTG+AGG - chr2_3:57123541-57123560 Msa0301280:five_prime_UTR 25.0%
! CTCACCAATAATATATTTGA+GGG - chr2_3:57123542-57123561 Msa0301280:five_prime_UTR 25.0%
CATAAGTTAAGGTAAATCTG+AGG + chr2_3:57121131-57121150 None:intergenic 30.0%
CTCAGATTTACCTTAACTTA+TGG - chr2_3:57121129-57121148 Msa0301280:three_prime_UTR 30.0%
GATTTACCTTAACTTATGGA+TGG - chr2_3:57121133-57121152 Msa0301280:three_prime_UTR 30.0%
TTAACTTATGGATGGAAGTT+GGG - chr2_3:57121141-57121160 Msa0301280:three_prime_UTR 30.0%
ATCATCTTTACAACAGGAAA+GGG - chr2_3:57121278-57121297 Msa0301280:CDS 30.0%
AATGGTATTCACAAACCTTA+TGG + chr2_3:57121390-57121409 None:intergenic 30.0%
GGAAAAAAAGAGGTGAAATT+AGG + chr2_3:57121484-57121503 None:intergenic 30.0%
CTTAGTTATTAGTAAGACCA+AGG + chr2_3:57121635-57121654 None:intergenic 30.0%
TTTAAAAAAGGAGCCAAATG+TGG + chr2_3:57122026-57122045 None:intergenic 30.0%
!!! TTAGATGCACTTTTTTGGAT+AGG - chr2_3:57122254-57122273 Msa0301280:intron 30.0%
!! TTTGATTTTCACTCCTACAA+AGG - chr2_3:57122400-57122419 Msa0301280:intron 30.0%
TAGCTAACTATATCCTTTGT+AGG + chr2_3:57122416-57122435 None:intergenic 30.0%
!! TTGGAAAAATTTGTTGGTAC+AGG - chr2_3:57122516-57122535 Msa0301280:intron 30.0%
GAACATCAATACATATTCAG+GGG + chr2_3:57122558-57122577 None:intergenic 30.0%
AAATGCTATTCGTCTATAGT+TGG + chr2_3:57122763-57122782 None:intergenic 30.0%
! CAAATTTGAGACTTAAACGT+TGG - chr2_3:57122957-57122976 Msa0301280:intron 30.0%
! GTTTTTCTATAGGTTTCATG+AGG - chr2_3:57123166-57123185 Msa0301280:CDS 30.0%
! CAGAATTTGATGACTTGAAA+AGG - chr2_3:57123192-57123211 Msa0301280:CDS 30.0%
ATTTGAATATGAAGGACTCA+AGG + chr2_3:57123341-57123360 None:intergenic 30.0%
CACATACAATACAATTGCAA+AGG + chr2_3:57123479-57123498 None:intergenic 30.0%
! CTATATAGAGCATTAGCTGA+GGG + chr2_3:57120935-57120954 None:intergenic 35.0%
! ACTATATAGAGCATTAGCTG+AGG + chr2_3:57120936-57120955 None:intergenic 35.0%
! TTTATTGGTTGCCAACTTGA+GGG + chr2_3:57120978-57120997 None:intergenic 35.0%
! ATTTATTGGTTGCCAACTTG+AGG + chr2_3:57120979-57120998 None:intergenic 35.0%
!! ACAAAAGGGTGCTCATTTAT+TGG + chr2_3:57120993-57121012 None:intergenic 35.0%
! AATAAATGAGCACCCTTTTG+TGG - chr2_3:57120992-57121011 Msa0301280:three_prime_UTR 35.0%
CAACTTCCATCCATAAGTTA+AGG + chr2_3:57121142-57121161 None:intergenic 35.0%
CTTAACTTATGGATGGAAGT+TGG - chr2_3:57121140-57121159 Msa0301280:three_prime_UTR 35.0%
TAACTTATGGATGGAAGTTG+GGG - chr2_3:57121142-57121161 Msa0301280:three_prime_UTR 35.0%
TGAAGTAGAAGTAGAAGAAG+AGG - chr2_3:57121207-57121226 Msa0301280:three_prime_UTR 35.0%
AAGAGGAAGATGTGAGTTAT+GGG - chr2_3:57121224-57121243 Msa0301280:three_prime_UTR 35.0%
TGACTGATCATCTTTACAAC+AGG - chr2_3:57121272-57121291 Msa0301280:CDS 35.0%
GATCATCTTTACAACAGGAA+AGG - chr2_3:57121277-57121296 Msa0301280:CDS 35.0%
CAGTTATCAACTTGACAAGA+AGG + chr2_3:57121328-57121347 None:intergenic 35.0%
GGTTTGTGAATACCATTCTA+AGG - chr2_3:57121393-57121412 Msa0301280:intron 35.0%
!! TTGAAGCTCTGAAATGAGTA+CGG + chr2_3:57121425-57121444 None:intergenic 35.0%
TCATTTCAGAGCTTCAACAA+AGG - chr2_3:57121428-57121447 Msa0301280:intron 35.0%
CCACTTCACATCAAATATTG+AGG + chr2_3:57121549-57121568 None:intergenic 35.0%
CCTCAATATTTGATGTGAAG+TGG - chr2_3:57121546-57121565 Msa0301280:intron 35.0%
AAATAGTAAGGGTCCACATT+TGG - chr2_3:57122010-57122029 Msa0301280:intron 35.0%
AGGAAAATGTCTGTAAGTTC+TGG - chr2_3:57122274-57122293 Msa0301280:intron 35.0%
GCCCAATTGGAAAAATTTGT+TGG - chr2_3:57122510-57122529 Msa0301280:intron 35.0%
AGCATGTTGGGGTAATTAAA+AGG + chr2_3:57122655-57122674 None:intergenic 35.0%
TAGCATAGAGTTACTGTGAT+TGG - chr2_3:57122723-57122742 Msa0301280:intron 35.0%
! TTTTGTGTTGTCAACTTCGT+GGG + chr2_3:57122828-57122847 None:intergenic 35.0%
GACACAGGTTAAAACTGATT+TGG + chr2_3:57122988-57123007 None:intergenic 35.0%
TGGTTAATTTAGCATGCACA+AGG + chr2_3:57123046-57123065 None:intergenic 35.0%
ATGACTTGAAAAGGAGTTGT+AGG - chr2_3:57123201-57123220 Msa0301280:CDS 35.0%
CAGCAAACTTAGCATAAAGA+GGG + chr2_3:57123412-57123431 None:intergenic 35.0%
TATTATTGGTGAGAAAGCCA+TGG + chr2_3:57123535-57123554 None:intergenic 35.0%
AACTTGAGGGTAACAGTGAT+GGG + chr2_3:57120965-57120984 None:intergenic 40.0%
TAGAATGGAAAAAGAGGGTG+TGG + chr2_3:57121019-57121038 None:intergenic 40.0%
CTTCTATACCAAGGTTGTTG+TGG - chr2_3:57121064-57121083 Msa0301280:three_prime_UTR 40.0%
TGAAAAGAGGGTGAAGTCAA+GGG + chr2_3:57121095-57121114 None:intergenic 40.0%
ATGAAAAGAGGGTGAAGTCA+AGG + chr2_3:57121096-57121115 None:intergenic 40.0%
GAAGAGGAAGATGTGAGTTA+TGG - chr2_3:57121223-57121242 Msa0301280:three_prime_UTR 40.0%
AGAGGAAGATGTGAGTTATG+GGG - chr2_3:57121225-57121244 Msa0301280:three_prime_UTR 40.0%
ATGTGAGTTATGGGGATGAT+GGG - chr2_3:57121233-57121252 Msa0301280:exon 40.0%
!! GGATGATGGGAAGAAGAAAA+GGG - chr2_3:57121246-57121265 Msa0301280:exon 40.0%
TATTTGATGTGAAGTGGCTG+AGG - chr2_3:57121552-57121571 Msa0301280:intron 40.0%
ATTTGATGTGAAGTGGCTGA+GGG - chr2_3:57121553-57121572 Msa0301280:intron 40.0%
CTATGAGTTGTCTCTTGCTA+GGG + chr2_3:57121658-57121677 None:intergenic 40.0%
GATTTAGCAACTTGCCCAAT+TGG - chr2_3:57122497-57122516 Msa0301280:intron 40.0%
TACATAGAACTCACACTCAC+AGG - chr2_3:57122594-57122613 Msa0301280:intron 40.0%
ACATAGAACTCACACTCACA+GGG - chr2_3:57122595-57122614 Msa0301280:intron 40.0%
TTTGTGTTGTCAACTTCGTG+GGG + chr2_3:57122827-57122846 None:intergenic 40.0%
! CTTTTGTGTTGTCAACTTCG+TGG + chr2_3:57122829-57122848 None:intergenic 40.0%
CACGAAGTTGACAACACAAA+AGG - chr2_3:57122827-57122846 Msa0301280:intron 40.0%
! TTGAAGAAGCATAAGGACAC+AGG + chr2_3:57123003-57123022 None:intergenic 40.0%
ACTTGAAAAGGAGTTGTAGG+AGG - chr2_3:57123204-57123223 Msa0301280:CDS 40.0%
! GATATTCAGACGTGCTTTTG+CGG + chr2_3:57123256-57123275 None:intergenic 40.0%
ACGTCTGAATATCACTGAGA+AGG - chr2_3:57123263-57123282 Msa0301280:CDS 40.0%
GCAGCAAACTTAGCATAAAG+AGG + chr2_3:57123413-57123432 None:intergenic 40.0%
CAACTTGAGGGTAACAGTGA+TGG + chr2_3:57120966-57120985 None:intergenic 45.0%
CATCACTGTTACCCTCAAGT+TGG - chr2_3:57120964-57120983 Msa0301280:three_prime_UTR 45.0%
GAAAAGAGGGTGAAGTCAAG+GGG + chr2_3:57121094-57121113 None:intergenic 45.0%
GATGTGAGTTATGGGGATGA+TGG - chr2_3:57121232-57121251 Msa0301280:exon 45.0%
!! GGGATGATGGGAAGAAGAAA+AGG - chr2_3:57121245-57121264 Msa0301280:exon 45.0%
TGATGGGAAGAAGAAAAGGG+TGG - chr2_3:57121249-57121268 Msa0301280:CDS 45.0%
AACAGGAAAGGGTCAAAAGC+TGG - chr2_3:57121289-57121308 Msa0301280:CDS 45.0%
CACAAACCTTATGGCGCTTA+TGG + chr2_3:57121381-57121400 None:intergenic 45.0%
GTACGGAATGAGCCTTAGAA+TGG + chr2_3:57121408-57121427 None:intergenic 45.0%
TTGTCAACCTGCTACATTGC+TGG + chr2_3:57121459-57121478 None:intergenic 45.0%
CCTATGAGTTGTCTCTTGCT+AGG + chr2_3:57121659-57121678 None:intergenic 45.0%
CCTAGCAAGAGACAACTCAT+AGG - chr2_3:57121656-57121675 Msa0301280:intron 45.0%
TTACCCCAACATGCTGAGAT+AGG - chr2_3:57122660-57122679 Msa0301280:intron 45.0%
TACCCCAACATGCTGAGATA+GGG - chr2_3:57122661-57122680 Msa0301280:intron 45.0%
ATACTTCATGGAGGCAAGAC+AGG - chr2_3:57122874-57122893 Msa0301280:intron 45.0%
!!! AATTTTGTAAAAATAAATAA+AGG - chr2_3:57122173-57122192 Msa0301280:intron 5.0%
!!! ATTTTGTAAAAATAAATAAA+GGG - chr2_3:57122174-57122193 Msa0301280:intron 5.0%
! ATAGAGCATTAGCTGAGGGC+CGG + chr2_3:57120931-57120950 None:intergenic 50.0%
! AGAGGGTGTGGACCACAAAA+GGG + chr2_3:57121007-57121026 None:intergenic 50.0%
! AAGAGGGTGTGGACCACAAA+AGG + chr2_3:57121008-57121027 None:intergenic 50.0%
TATGGATGGAAGTTGGGGTG+AGG - chr2_3:57121147-57121166 Msa0301280:three_prime_UTR 50.0%
ATGGATGGAAGTTGGGGTGA+GGG - chr2_3:57121148-57121167 Msa0301280:three_prime_UTR 50.0%
AGGAAAGGGTCAAAAGCTGG+AGG - chr2_3:57121292-57121311 Msa0301280:CDS 50.0%
GCAGTACCATAAGCGCCATA+AGG - chr2_3:57121372-57121391 Msa0301280:intron 50.0%
! GGTTTTGCCAGCAATGTAGC+AGG - chr2_3:57121449-57121468 Msa0301280:intron 50.0%
ACCCCAACATGCTGAGATAG+GGG - chr2_3:57122662-57122681 Msa0301280:intron 50.0%
TGCCCCTATCTCAGCATGTT+GGG + chr2_3:57122667-57122686 None:intergenic 50.0%
ATGCCCCTATCTCAGCATGT+TGG + chr2_3:57122668-57122687 None:intergenic 50.0%
!! AAGGAGTTGTAGGAGGCGTT+TGG - chr2_3:57123211-57123230 Msa0301280:CDS 50.0%
GTTTGGCTGGACATAACGAG+AGG - chr2_3:57123228-57123247 Msa0301280:CDS 50.0%
GGGGAAGACCACAACAACCT+TGG + chr2_3:57121075-57121094 None:intergenic 55.0%
GCCCCTATCTCAGCATGTTG+GGG + chr2_3:57122666-57122685 None:intergenic 55.0%
!! AGTTGTAGGAGGCGTTTGGC+TGG - chr2_3:57123215-57123234 Msa0301280:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 57120925 57123603 57120925 ID=Msa0301280;Name=Msa0301280
chr2_3 mRNA 57120925 57123603 57120925 ID=Msa0301280-mRNA-1;Parent=Msa0301280;Name=Msa0301280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=223|1|1|1|1|1|2|323|141
chr2_3 exon 57120925 57121350 57120925 ID=Msa0301280-mRNA-1:exon:18393;Parent=Msa0301280-mRNA-1
chr2_3 exon 57123058 57123603 57123058 ID=Msa0301280-mRNA-1:exon:18392;Parent=Msa0301280-mRNA-1
chr2_3 five_prime_UTR 57123381 57123603 57123381 ID=Msa0301280-mRNA-1:five_prime_utr;Parent=Msa0301280-mRNA-1
chr2_3 CDS 57123058 57123380 57123058 ID=Msa0301280-mRNA-1:cds;Parent=Msa0301280-mRNA-1
chr2_3 CDS 57121248 57121350 57121248 ID=Msa0301280-mRNA-1:cds;Parent=Msa0301280-mRNA-1
chr2_3 three_prime_UTR 57120925 57121247 57120925 ID=Msa0301280-mRNA-1:three_prime_utr;Parent=Msa0301280-mRNA-1
Gene Sequence

>Msa0301280

ATGGATGGAAGTTGGGGTGAGGGAAAGAGAATCTATGAGTACAGAGAAGAAGATGAGTATGAAGTAGAAGTAGAAGAAGAGGAAGATGTGAGTTATGGGGATGATGGGAAGAAGAAAAGGGTGGTGACTGATCATCTTTACAACAGGAAAGGGTCAAAAGCTGGAGGATCAGTGACACCTTCTTGTCAAGTTGATAACTGTAATGCTGATCTAAGTGTTGCTAAGCAGTACCATAAGCGCCATAAGGTTTGTGAATACCATTCTAAGGCTCATTCCGTACTCATTTCAGAGCTTCAACAAAGGTTTTGCCAGCAATGTAGCAGGTTTCATGAGGTATCAGAATTTGATGACTTGAAAAGGAGTTGTAGGAGGCGTTTGGCTGGACATAACGAGAGGCGCCGCAAAAGCACGTCTGAATATCACTGA
Protein sequence

>Msa0301280

MDGSWGEGKRIYEYREEDEYEVEVEEEEDVSYGDDGKKKRVVTDHLYNRKGSKAGGSVTPSCQVDNCNADLSVAKQYHKRHKVCEYHSKAHSVLISELQQRFCQQCSRFHEVSEFDDLKRSCRRRLAGHNERRRKSTSEYH*