AlfalfaGEDB | Msa0301280

M. sativa cultivar zhongmu-4 / Msa0301280

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301280	XP_003593617.1	91.608	143	10	1	1	141	1	143	2.79e-75	230

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301280	sp\|Q38741\|SBP1_ANTMA	58.865	141	48	3	1	141	1	131	2.46e-49	157

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0301280	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0118400	Msa0301280	0.804484	2.112490e-49	-8.615850e-47
Msa0192200	Msa0301280	0.942955	2.862015e-102	-8.615850e-47
Msa0192320	Msa0301280	0.981125	7.799045e-152	-8.615850e-47
Msa0301280	Msa0313080	0.904552	1.089063e-79	-8.615850e-47
Msa0301280	Msa0695410	0.812331	4.474760e-51	-8.615850e-47
Msa0301280	Msa1271280	0.800094	1.693536e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301280	MtrunA17_Chr2g0282521	91.608	143	10	1	1	141	1	143	2.56e-79	230
Msa0301280	MtrunA17_Chr4g0046991	64.085	142	48	2	1	140	11	151	9.95e-52	161
Msa0301280	MtrunA17_Chr8g0360361	66.667	81	27	0	59	139	62	142	1.53e-36	123
Msa0301280	MtrunA17_Chr7g0231911	70.238	84	25	0	54	137	141	224	1.20e-35	125
Msa0301280	MtrunA17_Chr8g0334671	67.470	83	27	0	54	136	179	261	3.88e-35	124
Msa0301280	MtrunA17_Chr5g0420051	61.053	95	37	0	46	140	165	259	5.62e-35	125
Msa0301280	MtrunA17_Chr1g0162871	56.410	117	40	3	23	136	70	178	1.10e-34	127
Msa0301280	MtrunA17_Chr7g0258471	67.059	85	24	1	55	135	131	215	1.55e-33	121
Msa0301280	MtrunA17_Chr2g0302791	69.333	75	23	0	62	136	139	213	2.65e-33	123
Msa0301280	MtrunA17_Chr4g0061651	70.886	79	23	0	57	135	118	196	7.93e-33	120

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301280	AT2G33810.1	70.000	90	24	1	53	139	42	131	2.50e-39	129
Msa0301280	AT1G53160.2	62.626	99	37	0	41	139	33	131	2.74e-38	128
Msa0301280	AT1G53160.1	62.626	99	37	0	41	139	33	131	2.74e-38	128
Msa0301280	AT1G53160.3	62.626	99	37	0	41	139	33	131	2.87e-38	128
Msa0301280	AT1G20980.1	68.539	89	26	1	49	137	109	195	7.14e-36	130
Msa0301280	AT1G76580.1	68.750	80	25	0	60	139	112	191	1.89e-34	126
Msa0301280	AT3G60030.1	63.830	94	29	2	50	139	112	204	4.39e-34	125
Msa0301280	AT1G02065.1	68.293	82	26	0	54	135	180	261	4.56e-34	121
Msa0301280	AT1G76580.3	68.750	80	25	0	60	139	112	191	8.65e-34	124
Msa0301280	AT3G15270.1	65.823	79	25	1	59	135	58	136	1.03e-33	116

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Find 71 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGGAAAGGGTCAAAAGC+TGG	0.479614	2_3:-57123217	Msa0301280:CDS
AACTTGAGGGTAACAGTGAT+GGG	0.587158	2_3:+57123544	None:intergenic
AAGAGGAAGATGTGAGTTAT+GGG	0.331465	2_3:-57123282	Msa0301280:CDS
AAGAGGGTGTGGACCACAAA+AGG	0.442762	2_3:+57123501	None:intergenic
AAGCCATGGTAATAAATTAA+TGG	0.327177	2_3:+57120988	None:intergenic
AAGGAGTTGTAGGAGGCGTT+TGG	0.456087	2_3:-57121295	Msa0301280:CDS
AATGGTATTCACAAACCTTA+TGG	0.308780	2_3:+57123119	None:intergenic
ACAAAAGGGTGCTCATTTAT+TGG	0.224690	2_3:+57123516	None:intergenic
ACGTCTGAATATCACTGAGA+AGG	0.591085	2_3:-57121243	Msa0301280:exon
ACTATATAGAGCATTAGCTG+AGG	0.670283	2_3:+57123573	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATAAGTTAAGGTAAATCTG+AGG	+	chr2_3:57121131-57121150	None:intergenic	30.0%
CTCAGATTTACCTTAACTTA+TGG	-	chr2_3:57121129-57121148	Msa0301280:three_prime_UTR	30.0%
GATTTACCTTAACTTATGGA+TGG	-	chr2_3:57121133-57121152	Msa0301280:three_prime_UTR	30.0%
TTAACTTATGGATGGAAGTT+GGG	-	chr2_3:57121141-57121160	Msa0301280:three_prime_UTR	30.0%
ATCATCTTTACAACAGGAAA+GGG	-	chr2_3:57121278-57121297	Msa0301280:CDS	30.0%
AATGGTATTCACAAACCTTA+TGG	+	chr2_3:57121390-57121409	None:intergenic	30.0%
GGAAAAAAAGAGGTGAAATT+AGG	+	chr2_3:57121484-57121503	None:intergenic	30.0%
CTTAGTTATTAGTAAGACCA+AGG	+	chr2_3:57121635-57121654	None:intergenic	30.0%
TTTAAAAAAGGAGCCAAATG+TGG	+	chr2_3:57122026-57122045	None:intergenic	30.0%
TAGCTAACTATATCCTTTGT+AGG	+	chr2_3:57122416-57122435	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	57120925	57123603	57120925	ID=Msa0301280;Name=Msa0301280
chr2_3	mRNA	57120925	57123603	57120925	ID=Msa0301280-mRNA-1;Parent=Msa0301280;Name=Msa0301280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=223\|1\|1\|1\|1\|1\|2\|323\|141
chr2_3	exon	57120925	57121350	57120925	ID=Msa0301280-mRNA-1:exon:18393;Parent=Msa0301280-mRNA-1
chr2_3	exon	57123058	57123603	57123058	ID=Msa0301280-mRNA-1:exon:18392;Parent=Msa0301280-mRNA-1
chr2_3	five_prime_UTR	57123381	57123603	57123381	ID=Msa0301280-mRNA-1:five_prime_utr;Parent=Msa0301280-mRNA-1
chr2_3	CDS	57123058	57123380	57123058	ID=Msa0301280-mRNA-1:cds;Parent=Msa0301280-mRNA-1
chr2_3	CDS	57121248	57121350	57121248	ID=Msa0301280-mRNA-1:cds;Parent=Msa0301280-mRNA-1
chr2_3	three_prime_UTR	57120925	57121247	57120925	ID=Msa0301280-mRNA-1:three_prime_utr;Parent=Msa0301280-mRNA-1

Gene Sequence

>Msa0301280

ATGGATGGAAGTTGGGGTGAGGGAAAGAGAATCTATGAGTACAGAGAAGAAGATGAGTATGAAGTAGAAGTAGAAGAAGAGGAAGATGTGAGTTATGGGGATGATGGGAAGAAGAAAAGGGTGGTGACTGATCATCTTTACAACAGGAAAGGGTCAAAAGCTGGAGGATCAGTGACACCTTCTTGTCAAGTTGATAACTGTAATGCTGATCTAAGTGTTGCTAAGCAGTACCATAAGCGCCATAAGGTTTGTGAATACCATTCTAAGGCTCATTCCGTACTCATTTCAGAGCTTCAACAAAGGTTTTGCCAGCAATGTAGCAGGTTTCATGAGGTATCAGAATTTGATGACTTGAAAAGGAGTTGTAGGAGGCGTTTGGCTGGACATAACGAGAGGCGCCGCAAAAGCACGTCTGAATATCACTGA

Protein sequence

>Msa0301280

MDGSWGEGKRIYEYREEDEYEVEVEEEEDVSYGDDGKKKRVVTDHLYNRKGSKAGGSVTPSCQVDNCNADLSVAKQYHKRHKVCEYHSKAHSVLISELQQRFCQQCSRFHEVSEFDDLKRSCRRRLAGHNERRRKSTSEYH*