AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0193970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0193970 MtrunA17_Chr2g0284361 93.137 102 7 0 1 102 1 102 7.54e-66 193
Msa0193970 MtrunA17_Chr8g0379531 57.732 97 34 2 9 102 8 100 5.91e-33 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0193970 AT1G29290.1 36.634 101 52 2 9 102 12 107 7.84e-12 57.4

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGCTTGGTGCAGAATA+TGG 0.235499 2_1:-9810171 None:intergenic
GAATATGGAACTTTGCCTTT+AGG 0.334242 2_1:-9810156 None:intergenic
TCCCTTTGTTCATGTCTAGA+AGG 0.367085 2_1:+9810051 Msa0193970:CDS
GAAGGAAGAAAGCTTCATAT+TGG 0.368317 2_1:+9810069 Msa0193970:CDS
AACAAATTGTCCCTTGAAGA+TGG 0.411417 2_1:-9810120 None:intergenic
CTTCGATCTGTTCCTAGTCC+TGG 0.413490 2_1:+9810273 Msa0193970:CDS
CAGCAGGTTCGGTGCTTATG+AGG 0.439064 2_1:-9810236 None:intergenic
AATCCGTACTTCAGCAGGTT+CGG 0.461110 2_1:-9810247 None:intergenic
ACAGAGTTACCTCTCTTGCT+TGG 0.463840 2_1:-9810183 None:intergenic
TTCTTGACTTCACTTCCTAA+AGG 0.476696 2_1:+9810141 Msa0193970:CDS
AGAAGAATCCGTACTTCAGC+AGG 0.481211 2_1:-9810252 None:intergenic
TCCTTCTAGACATGAACAAA+GGG 0.484605 2_1:-9810052 None:intergenic
AGAAAATTGACCCATCTTCA+AGG 0.491293 2_1:+9810109 Msa0193970:CDS
TCTGTTCCTAGTCCTGGTGC+TGG 0.534087 2_1:+9810279 Msa0193970:CDS
TTCCTTCTAGACATGAACAA+AGG 0.543888 2_1:-9810053 None:intergenic
ATCATTCAATGTCCAGCACC+AGG 0.555598 2_1:-9810291 None:intergenic
CAATGTCCAGCACCAGGACT+AGG 0.555682 2_1:-9810285 None:intergenic
ACAAATTGTCCCTTGAAGAT+GGG 0.568936 2_1:-9810119 None:intergenic
GCACCGAACCTGCTGAAGTA+CGG 0.569118 2_1:+9810244 Msa0193970:CDS
GAAAATTGACCCATCTTCAA+GGG 0.591769 2_1:+9810110 Msa0193970:CDS
TATTCTGCACCAAGCAAGAG+AGG 0.618318 2_1:+9810174 Msa0193970:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCCTTCTAGACATGAACAAA+GGG - chr2_1:9810055-9810074 None:intergenic 35.0%
TTCCTTCTAGACATGAACAA+AGG - chr2_1:9810056-9810075 None:intergenic 35.0%
GAAGGAAGAAAGCTTCATAT+TGG + chr2_1:9810069-9810088 Msa0193970:CDS 35.0%
! TGAAGATGGGTCAATTTTCT+TGG - chr2_1:9810109-9810128 None:intergenic 35.0%
AGAAAATTGACCCATCTTCA+AGG + chr2_1:9810109-9810128 Msa0193970:CDS 35.0%
GAAAATTGACCCATCTTCAA+GGG + chr2_1:9810110-9810129 Msa0193970:CDS 35.0%
ACAAATTGTCCCTTGAAGAT+GGG - chr2_1:9810122-9810141 None:intergenic 35.0%
AACAAATTGTCCCTTGAAGA+TGG - chr2_1:9810123-9810142 None:intergenic 35.0%
TTCTTGACTTCACTTCCTAA+AGG + chr2_1:9810141-9810160 Msa0193970:CDS 35.0%
GAATATGGAACTTTGCCTTT+AGG - chr2_1:9810159-9810178 None:intergenic 35.0%
TCCCTTTGTTCATGTCTAGA+AGG + chr2_1:9810051-9810070 Msa0193970:CDS 40.0%
!! CTCTTGCTTGGTGCAGAATA+TGG - chr2_1:9810174-9810193 None:intergenic 45.0%
TATTCTGCACCAAGCAAGAG+AGG + chr2_1:9810174-9810193 Msa0193970:CDS 45.0%
ACAGAGTTACCTCTCTTGCT+TGG - chr2_1:9810186-9810205 None:intergenic 45.0%
AATCCGTACTTCAGCAGGTT+CGG - chr2_1:9810250-9810269 None:intergenic 45.0%
AGAAGAATCCGTACTTCAGC+AGG - chr2_1:9810255-9810274 None:intergenic 45.0%
CTTCGATCTGTTCCTAGTCC+TGG + chr2_1:9810273-9810292 Msa0193970:CDS 50.0%
!! CAGCAGGTTCGGTGCTTATG+AGG - chr2_1:9810239-9810258 None:intergenic 55.0%
GCACCGAACCTGCTGAAGTA+CGG + chr2_1:9810244-9810263 Msa0193970:CDS 55.0%
TCTGTTCCTAGTCCTGGTGC+TGG + chr2_1:9810279-9810298 Msa0193970:CDS 55.0%
CAATGTCCAGCACCAGGACT+AGG - chr2_1:9810288-9810307 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 9810000 9810308 9810000 ID=Msa0193970;Name=Msa0193970
chr2_1 mRNA 9810000 9810308 9810000 ID=Msa0193970-mRNA-1;Parent=Msa0193970;Name=Msa0193970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|102
chr2_1 exon 9810000 9810308 9810000 ID=Msa0193970-mRNA-1:exon:4490;Parent=Msa0193970-mRNA-1
chr2_1 CDS 9810000 9810308 9810000 ID=Msa0193970-mRNA-1:cds;Parent=Msa0193970-mRNA-1
Gene Sequence

>Msa0193970

ATGGGTCGTATTAGAAATATGCAACTAGTTATATTCTTTATTCTCTTTGTTTCCCTTTGTTCATGTCTAGAAGGAAGAAAGCTTCATATTGGTTCAAGCAAAGAAACCAAGAAAATTGACCCATCTTCAAGGGACAATTTGTTCTTGACTTCACTTCCTAAAGGCAAAGTTCCATATTCTGCACCAAGCAAGAGAGGTAACTCTGTTGAAGTTGATGAGAAGCTTGTTGCACGTCACCTCATAAGCACCGAACCTGCTGAAGTACGGATTCTTCTTCGATCTGTTCCTAGTCCTGGTGCTGGACATTGA

Protein sequence

>Msa0193970

MGRIRNMQLVIFFILFVSLCSCLEGRKLHIGSSKETKKIDPSSRDNLFLTSLPKGKVPYSAPSKRGNSVEVDEKLVARHLISTEPAEVRILLRSVPSPGAGH*