AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1221220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221220 MtrunA17_Chr4g0072541 97.727 132 3 0 1 132 1 132 7.68e-93 264
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221220 AT5G57510.1 48.000 125 59 5 6 127 3 124 7.20e-26 94.7

Find 19 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGGGAACAAAAGAGTTC+TGG 0.299960 8_1:-84770315 Msa1221220:CDS
CATTGCAGCTACGTTATTGC+AGG 0.354632 8_1:-84770332 Msa1221220:CDS
CTCCATCTCCTTGTCTTCAA+AGG 0.416512 8_1:-84770452 Msa1221220:CDS
GTTCCATGTGTGGTTGAGTA+TGG 0.434971 8_1:+84770587 None:intergenic
CCTACGAACGGCGGTGCAAA+AGG 0.478843 8_1:-84770631 Msa1221220:CDS
CTTATTGTCCTTTGAAGACA+AGG 0.489660 8_1:+84770444 None:intergenic
CTAAGTTTCAACGATCGTCC+TGG 0.511331 8_1:-84770522 Msa1221220:CDS
GTCCTTTGAAGACAAGGAGA+TGG 0.521798 8_1:+84770450 None:intergenic
TTCAGATGAAACTGATTCAG+AGG 0.544944 8_1:-84770484 Msa1221220:CDS
CCACCATACTCAACCACACA+TGG 0.550819 8_1:-84770590 Msa1221220:CDS
TCAGATGAAACTGATTCAGA+GGG 0.570568 8_1:-84770483 Msa1221220:CDS
GAAGAACACATCATCAATCC+AGG 0.580359 8_1:+84770504 None:intergenic
CCATGTGTGGTTGAGTATGG+TGG 0.585997 8_1:+84770590 None:intergenic
TTTGCACCGCCGTTCGTAGG+CGG 0.592132 8_1:+84770634 None:intergenic
GGATGACATTGATAAGAGAT+CGG 0.600101 8_1:-84770406 Msa1221220:CDS
TCAATGTCATCCTCATCTGA+AGG 0.601031 8_1:+84770417 None:intergenic
ATTGCAGCTACGTTATTGCA+GGG 0.621027 8_1:-84770331 Msa1221220:CDS
TCTACTAAGCCGCCTACGAA+CGG 0.672450 8_1:-84770643 Msa1221220:CDS
ACTAAGCCGCCTACGAACGG+CGG 0.714763 8_1:-84770640 Msa1221220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATAAGTTTTCCTTCAGATG+AGG - chr8_1:84770547-84770566 Msa1221220:CDS 30.0%
! TTCAGATGAAACTGATTCAG+AGG - chr8_1:84770490-84770509 Msa1221220:CDS 35.0%
! TCAGATGAAACTGATTCAGA+GGG - chr8_1:84770491-84770510 Msa1221220:CDS 35.0%
! CTTATTGTCCTTTGAAGACA+AGG + chr8_1:84770533-84770552 None:intergenic 35.0%
GGATGACATTGATAAGAGAT+CGG - chr8_1:84770568-84770587 Msa1221220:CDS 35.0%
GAAGAACACATCATCAATCC+AGG + chr8_1:84770473-84770492 None:intergenic 40.0%
TCAATGTCATCCTCATCTGA+AGG + chr8_1:84770560-84770579 None:intergenic 40.0%
ATTGCAGCTACGTTATTGCA+GGG - chr8_1:84770643-84770662 Msa1221220:CDS 40.0%
GTTCCATGTGTGGTTGAGTA+TGG + chr8_1:84770390-84770409 None:intergenic 45.0%
! TTTTTGCAGCGTTCCATGTG+TGG + chr8_1:84770400-84770419 None:intergenic 45.0%
AACGCTGCAAAAATGCTTCG+CGG - chr8_1:84770407-84770426 Msa1221220:CDS 45.0%
CTAAGTTTCAACGATCGTCC+TGG - chr8_1:84770452-84770471 Msa1221220:CDS 45.0%
CTCCATCTCCTTGTCTTCAA+AGG - chr8_1:84770522-84770541 Msa1221220:CDS 45.0%
! GTCCTTTGAAGACAAGGAGA+TGG + chr8_1:84770527-84770546 None:intergenic 45.0%
CATTGCAGCTACGTTATTGC+AGG - chr8_1:84770642-84770661 Msa1221220:CDS 45.0%
TGCAGGGAACAAAAGAGTTC+TGG - chr8_1:84770659-84770678 Msa1221220:CDS 45.0%
TCTACTAAGCCGCCTACGAA+CGG - chr8_1:84770331-84770350 Msa1221220:CDS 50.0%
CCATGTGTGGTTGAGTATGG+TGG + chr8_1:84770387-84770406 None:intergenic 50.0%
CCACCATACTCAACCACACA+TGG - chr8_1:84770384-84770403 Msa1221220:CDS 50.0%
ACTAAGCCGCCTACGAACGG+CGG - chr8_1:84770334-84770353 Msa1221220:CDS 60.0%
TTTGCACCGCCGTTCGTAGG+CGG + chr8_1:84770343-84770362 None:intergenic 60.0%
! CCTTTTGCACCGCCGTTCGT+AGG + chr8_1:84770346-84770365 None:intergenic 60.0%
CCTACGAACGGCGGTGCAAA+AGG - chr8_1:84770343-84770362 Msa1221220:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 84770299 84770697 84770299 ID=Msa1221220;Name=Msa1221220
chr8_1 mRNA 84770299 84770697 84770299 ID=Msa1221220-mRNA-1;Parent=Msa1221220;Name=Msa1221220-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|132
chr8_1 exon 84770299 84770697 84770299 ID=Msa1221220-mRNA-1:exon:30457;Parent=Msa1221220-mRNA-1
chr8_1 CDS 84770299 84770697 84770299 ID=Msa1221220-mRNA-1:cds;Parent=Msa1221220-mRNA-1
Gene Sequence

>Msa1221220

ATGAGCACACACAATCAAAAGCAGAACAAGTCTCTACTAAGCCGCCTACGAACGGCGGTGCAAAAGGTGAAGCTTCTTATAAGCTCCACCATACTCAACCACACATGGAACGCTGCAAAAATGCTTCGCGGTGCTTCTTTGAGCAATCGACAACTAAGTTTCAACGATCGTCCTGGATTGATGATGTGTTCTTCAGATGAAACTGATTCAGAGGGTTCTGTTTCTCCATCTCCTTGTCTTCAAAGGACAATAAGTTTTCCTTCAGATGAGGATGACATTGATAAGAGATCGGAGATTTTCATAGCTAATTTCAGACGACAACTTTTGTTAGAGAGACAAATCTCATTGCAGCTACGTTATTGCAGGGAACAAAAGAGTTCTGGATTGATATCTCCTTGA

Protein sequence

>Msa1221220

MSTHNQKQNKSLLSRLRTAVQKVKLLISSTILNHTWNAAKMLRGASLSNRQLSFNDRPGLMMCSSDETDSEGSVSPSPCLQRTISFPSDEDDIDKRSEIFIANFRRQLLLERQISLQLRYCREQKSSGLISP*