AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0195560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195560 MtrunA17_Chr2g0286721 96.885 321 10 0 1 321 1 321 0.0 637
Msa0195560 MtrunA17_Chr4g0066581 81.505 319 56 1 1 316 1 319 0.0 540
Msa0195560 MtrunA17_Chr7g0259471 53.628 317 140 2 3 319 1 310 8.65e-117 346
Msa0195560 MtrunA17_Chr7g0259221 56.113 319 133 3 1 319 1 312 3.35e-116 345
Msa0195560 MtrunA17_Chr7g0273281 53.903 269 124 0 52 320 65 333 5.05e-99 301
Msa0195560 MtrunA17_Chr8g0373181 46.732 306 156 3 20 319 13 317 7.12e-90 277
Msa0195560 MtrunA17_Chr1g0155221 43.648 307 165 2 20 319 14 319 2.50e-86 268
Msa0195560 MtrunA17_Chr5g0404191 46.290 283 140 3 6 281 5 282 3.58e-81 249
Msa0195560 MtrunA17_Chr8g0373191 37.778 315 168 2 7 321 5 291 1.12e-71 229
Msa0195560 MtrunA17_Chr7g0273271 55.249 181 81 0 48 228 26 206 2.27e-69 216
Msa0195560 MtrunA17_Chr6g0457781 32.653 294 180 6 40 321 95 382 1.89e-35 135
Msa0195560 MtrunA17_Chr7g0250881 31.250 304 197 5 26 321 43 342 1.68e-33 129
Msa0195560 MtrunA17_Chr2g0303731 32.237 304 188 7 49 339 26 324 2.53e-32 127
Msa0195560 MtrunA17_Chr8g0376611 31.474 251 159 4 98 339 11 257 1.29e-29 118
Msa0195560 MtrunA17_Chr1g0213631 33.645 214 134 4 109 315 48 260 2.28e-29 117
Msa0195560 MtrunA17_Chr4g0021771 25.705 319 224 3 12 318 19 336 3.43e-29 117
Msa0195560 MtrunA17_Chr6g0486021 29.730 296 191 5 38 322 39 328 1.14e-28 115
Msa0195560 MtrunA17_Chr1g0213611 35.294 221 134 5 109 321 141 360 4.60e-28 114
Msa0195560 MtrunA17_Chr7g0214451 33.790 219 131 4 107 315 94 308 7.93e-28 113
Msa0195560 MtrunA17_Chr8g0376631 31.687 243 151 5 107 339 87 324 1.22e-27 113
Msa0195560 MtrunA17_Chr2g0282031 30.040 253 163 4 77 316 81 332 8.69e-27 110
Msa0195560 MtrunA17_Chr2g0303701 29.832 238 155 5 109 339 89 321 8.75e-27 110
Msa0195560 MtrunA17_Chr3g0115721 29.237 236 160 3 89 318 82 316 1.60e-26 109
Msa0195560 MtrunA17_Chr3g0115741 29.915 234 157 3 89 316 85 317 1.04e-25 107
Msa0195560 MtrunA17_Chr4g0034681 29.329 283 170 6 60 316 41 319 2.61e-25 106
Msa0195560 MtrunA17_Chr1g0205671 31.667 240 147 5 90 318 84 317 1.01e-24 104
Msa0195560 MtrunA17_Chr3g0085971 30.000 250 166 4 73 315 66 313 2.19e-24 103
Msa0195560 MtrunA17_Chr8g0390521 26.821 302 195 5 37 316 3 300 4.67e-24 102
Msa0195560 MtrunA17_Chr3g0144361 27.652 264 167 4 77 316 56 319 1.13e-23 101
Msa0195560 MtrunA17_Chr1g0205681 30.041 243 153 5 87 318 82 318 1.24e-23 101
Msa0195560 MtrunA17_Chr4g0039651 25.155 322 211 5 24 315 13 334 1.80e-23 100
Msa0195560 MtrunA17_Chr3g0119401 29.268 287 173 9 55 322 99 374 2.89e-23 100
Msa0195560 MtrunA17_Chr4g0004821 29.577 284 183 7 50 322 37 314 3.44e-23 100
Msa0195560 MtrunA17_Chr3g0115711 27.778 234 161 4 88 315 81 312 6.99e-23 99.4
Msa0195560 MtrunA17_Chr4g0032021 28.025 314 194 8 30 315 79 388 1.02e-22 99.0
Msa0195560 MtrunA17_Chr3g0078791 29.004 231 153 6 89 311 28 255 1.08e-22 98.2
Msa0195560 MtrunA17_Chr3g0128081 28.395 324 197 11 36 329 9 327 1.24e-22 98.6
Msa0195560 MtrunA17_Chr1g0164871 26.752 314 194 6 28 311 2 309 1.30e-22 98.6
Msa0195560 MtrunA17_Chr1g0164851 26.752 314 194 6 28 311 2 309 1.61e-22 98.6
Msa0195560 MtrunA17_Chr3g0144261 33.163 196 117 4 77 262 32 223 2.06e-22 98.6
Msa0195560 MtrunA17_Chr4g0048051 28.870 239 154 6 109 339 103 333 2.61e-22 97.8
Msa0195560 MtrunA17_Chr8g0376601 28.512 242 156 5 109 339 89 324 4.00e-22 97.4
Msa0195560 MtrunA17_Chr6g0485661 27.046 281 190 5 50 316 59 338 4.52e-22 97.1
Msa0195560 MtrunA17_Chr5g0394341 31.557 244 142 8 90 318 84 317 5.62e-22 96.7
Msa0195560 MtrunA17_Chr2g0290181 29.018 224 143 5 107 316 88 309 6.07e-22 97.1
Msa0195560 MtrunA17_Chr5g0420841 29.915 234 155 4 89 315 82 313 6.34e-22 96.7
Msa0195560 MtrunA17_Chr5g0432581 28.358 268 164 8 77 318 47 312 7.05e-22 96.3
Msa0195560 MtrunA17_Chr3g0115751 29.185 233 158 3 89 315 87 318 7.23e-22 96.3
Msa0195560 MtrunA17_Chr3g0115781 27.311 238 158 4 89 316 84 316 1.12e-21 95.9
Msa0195560 MtrunA17_Chr3g0128171 28.571 238 159 6 89 318 36 270 1.17e-21 95.5
Msa0195560 MtrunA17_Chr4g0036021 26.132 287 183 5 55 316 33 315 3.40e-21 94.4
Msa0195560 MtrunA17_Chr5g0437021 28.870 239 155 4 89 318 84 316 2.07e-20 92.0
Msa0195560 MtrunA17_Chr1g0205691 30.417 240 156 5 90 321 84 320 2.45e-20 92.0
Msa0195560 MtrunA17_Chr4g0065311 26.959 319 191 10 29 315 2 310 3.41e-20 91.3
Msa0195560 MtrunA17_Chr4g0065324 28.235 255 164 7 89 332 88 334 1.13e-19 89.7
Msa0195560 MtrunA17_Chr8g0390141 32.365 241 142 8 89 316 86 318 1.69e-19 89.4
Msa0195560 MtrunA17_Chr4g0048411 29.536 237 147 7 109 333 103 331 1.88e-19 89.4
Msa0195560 MtrunA17_Chr5g0417611 27.119 236 159 8 89 315 84 315 2.93e-19 88.6
Msa0195560 MtrunA17_Chr8g0373531 28.148 270 158 10 77 316 47 310 7.53e-19 87.4
Msa0195560 MtrunA17_Chr3g0078971 27.483 302 179 9 48 316 22 316 1.62e-18 86.7
Msa0195560 MtrunA17_Chr5g0434231 32.075 159 88 2 98 255 14 153 3.34e-18 80.5
Msa0195560 MtrunA17_Chr7g0257631 28.862 246 152 9 82 315 116 350 4.02e-18 85.5
Msa0195560 MtrunA17_Chr5g0445601 26.695 236 155 6 91 315 88 316 1.47e-17 83.6
Msa0195560 MtrunA17_Chr6g0486681 29.289 239 150 7 89 315 86 317 1.95e-17 83.2
Msa0195560 MtrunA17_Chr6g0486671 29.289 239 150 7 89 315 86 317 1.95e-17 83.2
Msa0195560 MtrunA17_Chr8g0390261 28.395 243 155 7 89 319 86 321 3.05e-17 82.8
Msa0195560 MtrunA17_Chr5g0419321 27.638 199 134 4 63 254 50 245 3.08e-17 82.8
Msa0195560 MtrunA17_Chr3g0146061 34.783 161 97 3 97 249 54 214 2.76e-16 80.1
Msa0195560 MtrunA17_Chr5g0403831 24.324 333 209 9 13 318 7 323 3.02e-16 79.7
Msa0195560 MtrunA17_Chr5g0407441 32.500 160 96 4 104 254 61 217 1.03e-15 78.2
Msa0195560 MtrunA17_Chr3g0078991 30.000 190 114 6 140 316 134 317 2.00e-14 74.3
Msa0195560 MtrunA17_Chr2g0290161 26.027 219 130 6 107 316 21 216 3.02e-14 73.6
Msa0195560 MtrunA17_Chr8g0370141 27.094 203 132 7 66 254 17 217 1.21e-13 71.2
Msa0195560 MtrunA17_Chr3g0115731 26.047 215 130 6 107 318 107 295 3.15e-12 67.4
Msa0195560 MtrunA17_Chr4g0065351 29.379 177 106 7 167 332 1 169 4.16e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195560 AT5G18840.1 67.722 316 95 2 1 315 1 310 2.60e-148 427
Msa0195560 AT2G48020.4 61.204 299 113 1 25 320 1 299 2.61e-132 383
Msa0195560 AT2G48020.3 61.204 299 113 1 25 320 1 299 3.18e-132 381
Msa0195560 AT2G48020.2 61.204 299 113 1 25 320 1 299 4.23e-131 382
Msa0195560 AT2G48020.1 61.204 299 113 1 25 320 1 299 4.23e-131 382
Msa0195560 AT3G05150.2 56.401 289 124 1 34 322 22 308 7.93e-112 334
Msa0195560 AT3G05150.1 54.455 303 131 2 34 336 22 317 8.61e-112 333
Msa0195560 AT3G05150.3 54.455 303 131 2 34 336 22 317 8.61e-112 333
Msa0195560 AT1G54730.11 55.056 267 120 0 54 320 37 303 1.70e-107 318
Msa0195560 AT1G54730.10 55.056 267 120 0 54 320 37 303 2.11e-107 317
Msa0195560 AT1G54730.6 55.056 267 120 0 54 320 37 303 2.11e-107 317
Msa0195560 AT1G54730.8 55.056 267 120 0 54 320 37 303 9.93e-107 318
Msa0195560 AT1G54730.9 55.056 267 120 0 54 320 37 303 2.34e-106 318
Msa0195560 AT1G54730.7 55.056 267 120 0 54 320 37 303 2.34e-106 318
Msa0195560 AT1G54730.4 55.056 267 120 0 54 320 37 303 4.34e-106 317
Msa0195560 AT1G54730.3 55.056 267 120 0 54 320 37 303 8.98e-106 317
Msa0195560 AT1G54730.5 55.056 267 120 0 54 320 37 303 9.50e-106 317
Msa0195560 AT1G54730.2 55.056 267 120 0 54 320 37 303 2.40e-105 317
Msa0195560 AT1G08920.3 51.273 275 134 0 48 322 31 305 3.46e-94 288
Msa0195560 AT1G08920.1 51.273 275 134 0 48 322 31 305 6.84e-94 288
Msa0195560 AT1G08920.2 51.273 275 134 0 48 322 31 305 7.03e-94 288
Msa0195560 AT1G08930.1 44.134 358 175 6 3 336 1 357 9.32e-92 283
Msa0195560 AT1G08930.2 44.134 358 175 6 3 336 1 357 9.32e-92 283
Msa0195560 AT3G20460.1 44.514 319 168 4 3 318 10 322 1.33e-89 277
Msa0195560 AT1G75220.1 42.638 326 180 2 20 339 14 338 2.40e-87 271
Msa0195560 AT3G05165.4 47.080 274 145 0 49 322 30 303 1.72e-86 268
Msa0195560 AT3G05165.5 47.080 274 145 0 49 322 30 303 1.72e-86 268
Msa0195560 AT3G05165.3 47.080 274 145 0 49 322 30 303 3.39e-86 268
Msa0195560 AT3G05165.2 47.080 274 145 0 49 322 30 303 3.39e-86 268
Msa0195560 AT3G05165.1 47.080 274 145 0 49 322 30 303 3.39e-86 268
Msa0195560 AT3G05165.6 47.080 274 145 0 49 322 30 303 1.28e-85 268
Msa0195560 AT1G19450.1 42.453 318 172 3 10 319 5 319 3.11e-85 266
Msa0195560 AT3G05155.2 44.558 294 149 2 36 318 3 293 1.59e-84 259
Msa0195560 AT3G05160.1 47.445 274 144 0 49 322 21 294 1.72e-84 263
Msa0195560 AT5G27350.1 47.810 274 143 0 49 322 31 304 2.00e-84 263
Msa0195560 AT5G27360.4 48.315 267 138 0 49 315 35 301 2.84e-83 259
Msa0195560 AT5G27360.1 48.315 267 138 0 49 315 35 301 1.21e-82 259
Msa0195560 AT3G05155.1 44.558 294 149 2 36 318 3 293 2.91e-82 257
Msa0195560 AT5G27360.3 48.315 267 138 0 49 315 83 349 4.23e-82 259
Msa0195560 AT4G04760.2 46.691 272 142 1 50 318 32 303 7.55e-81 254
Msa0195560 AT3G05400.1 46.667 270 144 0 48 317 25 294 8.47e-81 254
Msa0195560 AT4G04760.1 46.691 272 142 1 50 318 32 303 1.07e-80 254
Msa0195560 AT4G04750.1 44.964 278 151 1 41 316 28 305 2.65e-77 245
Msa0195560 AT4G04750.2 44.964 278 151 1 41 316 28 305 2.65e-77 245
Msa0195560 AT4G04750.3 44.643 280 153 1 41 318 72 351 4.15e-77 246
Msa0195560 AT1G08890.1 45.804 286 151 1 33 318 15 296 2.64e-76 242
Msa0195560 AT3G05160.3 46.502 243 130 0 80 322 3 245 2.94e-72 230
Msa0195560 AT3G05160.2 46.502 243 130 0 80 322 3 245 2.94e-72 230
Msa0195560 AT1G08900.4 45.185 270 148 0 49 318 25 294 3.26e-72 231
Msa0195560 AT1G08900.1 45.185 270 148 0 49 318 25 294 9.43e-72 230
Msa0195560 AT1G08900.2 45.185 270 148 0 49 318 25 294 9.43e-72 230
Msa0195560 AT3G05400.2 44.444 270 130 2 48 317 25 274 4.79e-70 225
Msa0195560 AT4G04760.3 46.281 242 127 1 80 318 3 244 2.32e-69 223
Msa0195560 AT1G08900.5 44.444 252 140 0 67 318 1 252 1.01e-65 213
Msa0195560 AT1G08900.3 43.704 270 144 1 49 318 25 286 3.81e-65 213
Msa0195560 AT5G27360.2 46.701 197 105 0 119 315 1 197 3.98e-55 184
Msa0195560 AT5G27360.5 46.701 197 105 0 119 315 1 197 3.98e-55 184
Msa0195560 AT5G16150.2 32.131 305 194 5 26 321 82 382 3.29e-36 137
Msa0195560 AT5G16150.1 32.131 305 194 5 26 321 82 382 3.29e-36 137
Msa0195560 AT5G16150.3 32.131 305 194 5 26 321 82 382 3.29e-36 137
Msa0195560 AT1G30220.1 33.225 307 181 10 49 339 28 326 2.29e-29 119
Msa0195560 AT1G05030.1 31.429 280 162 7 55 316 85 352 1.04e-28 116
Msa0195560 AT1G05030.2 32.636 239 135 5 93 316 73 300 3.13e-28 114
Msa0195560 AT2G43330.1 35.556 225 128 7 109 321 94 313 3.46e-27 112
Msa0195560 AT1G67300.4 29.618 314 180 7 33 322 35 331 1.64e-26 109
Msa0195560 AT1G67300.5 29.618 314 180 7 33 322 35 331 1.64e-26 109
Msa0195560 AT1G67300.6 29.618 314 180 7 33 322 35 331 2.15e-26 109
Msa0195560 AT1G67300.7 29.618 314 180 7 33 322 35 331 2.15e-26 109
Msa0195560 AT1G67300.1 29.618 314 180 7 33 322 35 331 2.67e-26 109
Msa0195560 AT1G67300.3 29.618 314 180 7 33 322 35 331 2.67e-26 109
Msa0195560 AT1G67300.2 29.618 314 180 7 33 322 35 331 2.74e-26 109
Msa0195560 AT5G17010.1 26.377 345 215 9 4 319 6 340 3.08e-26 109
Msa0195560 AT5G17010.3 26.377 345 215 9 4 319 6 340 3.08e-26 109
Msa0195560 AT5G17010.4 26.377 345 215 9 4 319 6 340 3.08e-26 109
Msa0195560 AT2G20780.1 28.521 284 185 4 49 318 55 334 5.96e-26 108
Msa0195560 AT3G05960.1 31.439 264 158 7 90 342 81 332 1.72e-25 107
Msa0195560 AT3G03090.1 27.164 335 209 8 9 319 17 340 2.83e-25 106
Msa0195560 AT5G26250.1 31.061 264 159 7 90 342 82 333 9.32e-25 105
Msa0195560 AT2G20780.2 29.020 255 163 4 78 318 26 276 9.44e-25 104
Msa0195560 AT1G07340.1 33.621 232 146 5 90 315 83 312 3.00e-24 103
Msa0195560 AT4G21480.1 32.051 234 152 5 87 315 81 312 4.36e-24 103
Msa0195560 AT1G79820.4 30.847 295 193 5 36 322 41 332 6.62e-24 102
Msa0195560 AT1G79820.2 30.667 300 187 6 36 322 41 332 9.40e-24 102
Msa0195560 AT1G79820.1 30.667 300 187 6 36 322 41 332 9.40e-24 102
Msa0195560 AT5G17010.2 24.601 313 201 7 21 298 8 320 1.39e-23 101
Msa0195560 AT1G11260.1 30.544 239 147 6 89 315 83 314 1.76e-23 101
Msa0195560 AT5G59250.1 27.848 316 201 8 30 319 80 394 1.97e-23 101
Msa0195560 AT5G23270.1 30.435 253 164 6 89 333 85 333 2.37e-23 101
Msa0195560 AT2G16120.1 27.113 284 176 5 60 316 37 316 2.84e-23 100
Msa0195560 AT3G19940.1 27.820 266 169 7 90 344 86 339 8.15e-23 99.8
Msa0195560 AT5G26340.1 32.353 238 142 6 90 315 85 315 8.44e-23 99.8
Msa0195560 AT2G16130.1 27.143 280 181 4 60 316 37 316 1.23e-22 99.0
Msa0195560 AT3G19930.1 27.273 264 176 6 89 344 82 337 2.10e-22 98.6
Msa0195560 AT3G19930.2 27.273 264 176 6 89 344 82 337 2.10e-22 98.6
Msa0195560 AT3G18830.1 26.298 289 182 5 55 316 42 326 3.69e-22 97.8
Msa0195560 AT2G35740.1 27.519 258 169 6 93 337 73 325 4.99e-22 97.4
Msa0195560 AT4G36670.1 28.322 286 180 6 55 316 23 307 3.65e-21 94.7
Msa0195560 AT4G16480.2 27.984 243 155 5 109 337 90 326 4.70e-21 94.7
Msa0195560 AT4G16480.3 27.984 243 155 5 109 337 90 326 4.70e-21 94.7
Msa0195560 AT4G16480.1 27.984 243 155 5 109 337 90 326 4.70e-21 94.7
Msa0195560 AT2G18480.1 25.979 281 177 5 63 316 36 312 5.66e-21 94.4
Msa0195560 AT1G77210.2 30.962 239 146 7 89 315 86 317 8.29e-21 93.6
Msa0195560 AT1G77210.1 30.962 239 146 7 89 315 86 317 8.29e-21 93.6
Msa0195560 AT1G50310.1 29.876 241 150 7 90 318 86 319 1.60e-20 92.8
Msa0195560 AT1G20840.2 29.148 223 144 4 51 263 6 224 4.08e-20 92.0
Msa0195560 AT1G20840.1 29.148 223 144 4 51 263 6 224 4.08e-20 92.0
Msa0195560 AT3G51490.1 35.714 168 94 4 97 254 54 217 5.94e-20 91.7
Msa0195560 AT3G51490.2 35.714 168 94 4 97 254 54 217 6.03e-20 91.7
Msa0195560 AT3G51490.3 35.714 168 94 4 97 254 4 167 6.55e-20 91.3
Msa0195560 AT4G35300.9 35.673 171 90 4 97 254 54 217 1.54e-19 90.5
Msa0195560 AT4G35300.2 35.673 171 90 4 97 254 54 217 1.54e-19 90.5
Msa0195560 AT4G35300.3 35.673 171 90 4 97 254 54 217 1.54e-19 90.5
Msa0195560 AT4G35300.4 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT4G35300.8 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT4G35300.6 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT4G35300.11 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT4G35300.1 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT4G35300.10 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT4G35300.7 34.078 179 98 4 97 262 54 225 1.73e-19 90.1
Msa0195560 AT1G54730.1 54.839 62 28 0 100 161 1 62 2.17e-17 75.9
Msa0195560 AT5G61520.1 30.435 184 112 5 142 315 142 319 3.06e-17 83.2
Msa0195560 AT5G61520.2 30.435 184 112 5 142 315 94 271 3.15e-17 82.8
Msa0195560 AT4G02050.2 31.868 182 106 6 145 315 142 316 1.01e-16 81.6
Msa0195560 AT4G02050.1 31.868 182 106 6 145 315 142 316 1.01e-16 81.6
Msa0195560 AT1G79820.3 31.696 224 134 4 36 246 41 258 5.57e-16 79.0
Msa0195560 AT1G34580.2 27.801 241 154 7 89 315 33 267 1.09e-15 78.2
Msa0195560 AT1G34580.1 27.801 241 154 7 89 315 83 317 1.36e-15 78.2
Msa0195560 AT1G77210.3 32.738 168 94 7 160 315 8 168 2.82e-13 70.5

Find 64 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAATCTGTTGGAATTAA+TGG 0.124064 2_1:+12395383 Msa0195560:CDS
TTGCAGCTTATGATTGTTAT+TGG 0.148748 2_1:+12394447 Msa0195560:intron
TCAGTAAGGCTTGCAGATTT+TGG 0.233753 2_1:-12395979 None:intergenic
TGATGAAGCTAATGAAATTC+TGG 0.251682 2_1:+12395063 Msa0195560:CDS
TCAGATTGGAGTTGGATTAA+TGG 0.253920 2_1:+12395355 Msa0195560:intron
TAATAGGAAGTCTCATAAAT+TGG 0.255488 2_1:+12394487 Msa0195560:CDS
AAGAATTTCAACTAGCATTA+AGG 0.257869 2_1:+12395011 Msa0195560:CDS
CCTTGCATTTGCTTGCTTGT+TGG 0.304574 2_1:+12394646 Msa0195560:CDS
AGCACATTTGTTGCTGTTTG+TGG 0.330585 2_1:+12391437 Msa0195560:CDS
TCAGATAAAAGTGTGGAAAA+TGG 0.337919 2_1:+12391392 Msa0195560:CDS
TTTGGATTGGCTAGGGCATT+TGG 0.339542 2_1:-12395961 None:intergenic
GCTATTTCTGCTATATATAC+TGG 0.345158 2_1:-12394307 None:intergenic
CACCCACTCAAGCGGCTATC+AGG 0.386134 2_1:+12392556 Msa0195560:CDS
CAGAGCGCACATATTTGCTT+TGG 0.390072 2_1:-12395237 None:intergenic
GCTAGCTGTCTTCTTCTCTA+AGG 0.393869 2_1:+12393696 Msa0195560:CDS
ATTGAGGATGGAGAAACTAA+TGG 0.405911 2_1:+12391308 Msa0195560:CDS
TCATTCCTGAGTCTCCTAGA+TGG 0.424540 2_1:+12394677 Msa0195560:CDS
TTGAGGATGGAGAAACTAAT+GGG 0.428281 2_1:+12391309 Msa0195560:CDS
GAACAATTTCAGATTGGAGT+TGG 0.429941 2_1:+12395347 Msa0195560:intron
GTGACCATTGGTGCAATGCT+TGG 0.438418 2_1:+12393498 Msa0195560:CDS
ATGGCATTTCAACAATCTGT+TGG 0.440440 2_1:+12395374 Msa0195560:CDS
CTGATATCATTGGCAGGAAA+GGG 0.440631 2_1:+12393544 Msa0195560:CDS
TCCTTTCCTTGTTGAATGAA+AGG 0.450559 2_1:-12391350 None:intergenic
AGGACTTGCAACAACAAATC+AGG 0.474061 2_1:+12394348 Msa0195560:CDS
AAGGATGCTTGTAAAGAAGT+TGG 0.476415 2_1:+12391368 Msa0195560:CDS
AACTAGCATTAAGGAAACTA+CGG 0.476801 2_1:+12395020 Msa0195560:CDS
TTTGGTTCTTTGGTGACCAT+TGG 0.481812 2_1:+12393486 Msa0195560:CDS
AATGACAATTGAGCAGCATA+AGG 0.485697 2_1:+12391283 None:intergenic
GAGTTCTTACACACTTGACT+TGG 0.485780 2_1:+12393816 Msa0195560:intron
GCAAGCAAATGCAAGGAACT+AGG 0.487157 2_1:-12394639 None:intergenic
AGTGTGGAAAATGGATCCAT+TGG 0.491014 2_1:+12391401 Msa0195560:CDS
TCCTGAGTCTCCTAGATGGC+TGG 0.502043 2_1:+12394681 Msa0195560:CDS
AGCTCCAAGCATTGCACCAA+TGG 0.502584 2_1:-12393502 None:intergenic
CAAGAACCTTTCATTCAACA+AGG 0.516918 2_1:+12391344 Msa0195560:CDS
TCCTTCCTTACCAGCCATCT+AGG 0.519483 2_1:-12394691 None:intergenic
ACTAGCATTAAGGAAACTAC+GGG 0.523883 2_1:+12395021 Msa0195560:CDS
GGTCGAGTTACTGATATCAT+TGG 0.527994 2_1:+12393534 Msa0195560:CDS
ACCTTTCATTCAACAAGGAA+AGG 0.530081 2_1:+12391349 Msa0195560:CDS
TCTGTTGGAATTAATGGCAT+AGG 0.532395 2_1:+12395389 Msa0195560:CDS
CTTACACACTTGACTTGGGA+AGG 0.536358 2_1:+12393821 Msa0195560:CDS
TGAGCAGCATAAGGATATTG+AGG 0.536915 2_1:+12391292 Msa0195560:CDS
ACTGATATCATTGGCAGGAA+AGG 0.545701 2_1:+12393543 Msa0195560:CDS
ACCAGCCATCTAGGAGACTC+AGG 0.545779 2_1:-12394682 None:intergenic
TTCGCCGCACTAATTCAGTA+AGG 0.550065 2_1:-12395993 None:intergenic
CAGCATAAGGATATTGAGGA+TGG 0.555700 2_1:+12391296 Msa0195560:CDS
TGGCGACAACTAGCTCTAGC+AGG 0.561924 2_1:+12394507 Msa0195560:CDS
GACCTGATAGCCGCTTGAGT+GGG 0.574441 2_1:-12392558 None:intergenic
GAGTTACTGATATCATTGGC+AGG 0.583613 2_1:+12393538 Msa0195560:CDS
AGTTCTTACACACTTGACTT+GGG 0.588739 2_1:+12393817 Msa0195560:CDS
TCCTATGATACAAAATCCTG+TGG 0.593679 2_1:-12393671 None:intergenic
AATTGGAGTTATCTCATATG+TGG 0.595991 2_1:+12393861 Msa0195560:CDS
AGACCTGATAGCCGCTTGAG+TGG 0.599979 2_1:-12392559 None:intergenic
CGAACTATCAACAAATTCTG+TGG 0.608925 2_1:+12396012 Msa0195560:CDS
CCAACAAGCAAGCAAATGCA+AGG 0.620888 2_1:-12394646 None:intergenic
CAGGCTATGAGAATTTCCAC+AGG 0.633545 2_1:+12393655 Msa0195560:intron
AGTTGGATCAGATAAAAGTG+TGG 0.634652 2_1:+12391385 Msa0195560:CDS
TTATTCAGCACCCACTCAAG+CGG 0.639807 2_1:+12392548 Msa0195560:CDS
ATGCTTGGAGCTATAACAAG+TGG 0.646646 2_1:+12393513 Msa0195560:CDS
GCTAAGCAGAACCATTCCAA+TGG 0.646752 2_1:-12391417 None:intergenic
TGATATCATTGGCAGGAAAG+GGG 0.647485 2_1:+12393545 Msa0195560:CDS
GGAAAATGGATCCATTGGAA+TGG 0.671020 2_1:+12391406 Msa0195560:CDS
CAAGCCTTACTGAATTAGTG+CGG 0.686735 2_1:+12395989 Msa0195560:CDS
TCTTAATCTATCTCTTGCTG+AGG 0.689216 2_1:+12392584 Msa0195560:CDS
CTAGCATTAAGGAAACTACG+GGG 0.788030 2_1:+12395022 Msa0195560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTTGTAAATTATTAT+TGG + chr2_1:12391493-12391512 Msa0195560:intron 10.0%
!! TTATCAATAAGAATTTAAAT+TGG - chr2_1:12392326-12392345 None:intergenic 10.0%
!! ATAATATAATAATGACAATA+AGG - chr2_1:12392633-12392652 None:intergenic 10.0%
!!! TTTTTTTATGCAATAATTTA+AGG - chr2_1:12393315-12393334 None:intergenic 10.0%
!!! TCTAAGAATAAAATTTTAAA+TGG - chr2_1:12393606-12393625 None:intergenic 10.0%
!!! AAAATTAATTTTAACATGTA+TGG + chr2_1:12393969-12393988 Msa0195560:intron 10.0%
!! TTAGAAATATTATAATAAAG+AGG - chr2_1:12394261-12394280 None:intergenic 10.0%
!!! ATTTAAAACTAATGTTATTA+TGG - chr2_1:12394538-12394557 None:intergenic 10.0%
!! ATCAATTAAAAAAAATACTA+TGG - chr2_1:12395120-12395139 None:intergenic 10.0%
!! AAATGTAATAAATACTATAA+TGG - chr2_1:12395655-12395674 None:intergenic 10.0%
!! CAACTTAATTACATTTAAAT+TGG + chr2_1:12391814-12391833 Msa0195560:intron 15.0%
!!! GATTTTGTTTATTTTTCTTT+TGG + chr2_1:12392010-12392029 Msa0195560:intron 15.0%
!!! AATAGGTTAAACTTTAAAAT+TGG + chr2_1:12393080-12393099 Msa0195560:intron 15.0%
!!! TATTTCTACATAATATGTTT+TGG + chr2_1:12393444-12393463 Msa0195560:intron 15.0%
!!! ATTTCTACATAATATGTTTT+GGG + chr2_1:12393445-12393464 Msa0195560:intron 15.0%
!! TTTAAATGGAAAGAATATTA+AGG - chr2_1:12393592-12393611 None:intergenic 15.0%
!!! TATTTTTATTCAATCATGTT+TGG + chr2_1:12393919-12393938 Msa0195560:intron 15.0%
!!! AATTTGAAATTTTTAGTTCT+AGG + chr2_1:12394029-12394048 Msa0195560:intron 15.0%
!!! AAAATTGATTTTGAATGAAT+TGG - chr2_1:12394161-12394180 None:intergenic 15.0%
!!! CATTATTCTTATTCTTTTAT+AGG - chr2_1:12394931-12394950 None:intergenic 15.0%
!!! TTAATCAATGACAAAATTTT+TGG + chr2_1:12395562-12395581 Msa0195560:intron 15.0%
!! ACAACATGATTAAACTTAAA+TGG + chr2_1:12391755-12391774 Msa0195560:intron 20.0%
!!! TTTATTTTTCTTTTGGTGTA+TGG + chr2_1:12392017-12392036 Msa0195560:intron 20.0%
!! AATATGTCAAAAATAGATGT+AGG - chr2_1:12392988-12393007 None:intergenic 20.0%
!! AGTAAAGTAAGCATTTAAAT+AGG + chr2_1:12393063-12393082 Msa0195560:intron 20.0%
!! AAAGTACAGAATTTGTAAAT+TGG + chr2_1:12393266-12393285 Msa0195560:intron 20.0%
!!! TTTCTACATAATATGTTTTG+GGG + chr2_1:12393446-12393465 Msa0195560:intron 20.0%
!!! TATTCTTAGAAGTAACATTT+TGG + chr2_1:12393616-12393635 Msa0195560:intron 20.0%
!!! TCATCAATGTCATTTTTAAT+AGG + chr2_1:12394471-12394490 Msa0195560:CDS 20.0%
!! AAGGATATAACAAATTAAGA+AGG + chr2_1:12394567-12394586 Msa0195560:intron 20.0%
!! AAAGAATAAGAATAATGTGT+CGG + chr2_1:12394934-12394953 Msa0195560:intron 20.0%
!!! AAATTATGCACAATTTTTTC+AGG + chr2_1:12394970-12394989 Msa0195560:intron 20.0%
!! GAAATTGTTCTATATAGTTT+AGG - chr2_1:12395337-12395356 None:intergenic 20.0%
!! TATATAGAACAATTTCAGAT+TGG + chr2_1:12395341-12395360 Msa0195560:intron 20.0%
!! TTAACATAGTGCTATATTTA+AGG - chr2_1:12395520-12395539 None:intergenic 20.0%
!!! GTAATAAATTTGTTGACAAT+TGG + chr2_1:12395589-12395608 Msa0195560:intron 20.0%
!! TATTTACACATTTACACATT+TGG - chr2_1:12395904-12395923 None:intergenic 20.0%
!! CGATTAAAAACAAGTCATTT+TGG - chr2_1:12391667-12391686 None:intergenic 25.0%
!!! ACTAGGCTGTAAATATTTTT+TGG - chr2_1:12391917-12391936 None:intergenic 25.0%
! CTGTTACAACAGATAAAATA+AGG - chr2_1:12392125-12392144 None:intergenic 25.0%
!! TTATTGATAAAGTGTTCGTA+AGG + chr2_1:12392336-12392355 Msa0195560:intron 25.0%
!! TCATCATATTTGTTGATAAG+TGG + chr2_1:12392702-12392721 Msa0195560:intron 25.0%
!! CATCATATTTGTTGATAAGT+GGG + chr2_1:12392703-12392722 Msa0195560:intron 25.0%
! AATAACATGTAATGTAGGTT+AGG - chr2_1:12392821-12392840 None:intergenic 25.0%
! CTTTCAATAACATGTAATGT+AGG - chr2_1:12392826-12392845 None:intergenic 25.0%
! AAAAAAAAGTCTAGCTACAT+GGG - chr2_1:12393153-12393172 None:intergenic 25.0%
! TAAAAAAAAGTCTAGCTACA+TGG - chr2_1:12393154-12393173 None:intergenic 25.0%
!! GTTAGAGAGTACAAAATTTA+TGG - chr2_1:12393293-12393312 None:intergenic 25.0%
! TGGATAATTATATGAGAAAC+AGG + chr2_1:12393636-12393655 Msa0195560:intron 25.0%
!! TTTTATTCAATCATGTTTGG+AGG + chr2_1:12393922-12393941 Msa0195560:intron 25.0%
! TGATTTGAAAGGTAAATTCA+AGG - chr2_1:12394067-12394086 None:intergenic 25.0%
! TAAGTGTATTGAATGTAAGA+TGG - chr2_1:12394115-12394134 None:intergenic 25.0%
! TCTAACAAAAATCAGCATAA+CGG + chr2_1:12394276-12394295 Msa0195560:intron 25.0%
! CTTCTTAAGTATCATTGAAT+TGG + chr2_1:12394400-12394419 Msa0195560:intron 25.0%
! TAATAGGAAGTCTCATAAAT+TGG + chr2_1:12394487-12394506 Msa0195560:CDS 25.0%
!!! AGTTTTAAATTTTTGCTCGA+AGG + chr2_1:12394548-12394567 Msa0195560:intron 25.0%
! TAGTTTATATGCGAAAAGTT+TGG + chr2_1:12394791-12394810 Msa0195560:intron 25.0%
! AAGAATTTCAACTAGCATTA+AGG + chr2_1:12395011-12395030 Msa0195560:CDS 25.0%
!! AAATCCAAAAACTTAGTCTT+AGG - chr2_1:12395214-12395233 None:intergenic 25.0%
! AATCTGTTTGATTACTCTAA+AGG + chr2_1:12395309-12395328 Msa0195560:intron 25.0%
! ATCTGTTTGATTACTCTAAA+GGG + chr2_1:12395310-12395329 Msa0195560:intron 25.0%
!! TAATTGTTGACTAAGTAACT+TGG + chr2_1:12395537-12395556 Msa0195560:intron 25.0%
! GCAAATTTCTCATTCTTAAA+TGG - chr2_1:12395633-12395652 None:intergenic 25.0%
!! CCTGATTATGTTACAATTTT+CGG - chr2_1:12395680-12395699 None:intergenic 25.0%
! CGAAAATTGTAACATAATCA+GGG + chr2_1:12395678-12395697 Msa0195560:intron 25.0%
!!! GGCATTTGTTTTTTCTTAAA+GGG - chr2_1:12395883-12395902 None:intergenic 25.0%
!!! TGGCATTTGTTTTTTCTTAA+AGG - chr2_1:12395884-12395903 None:intergenic 25.0%
! TCAGATAAAAGTGTGGAAAA+TGG + chr2_1:12391392-12391411 Msa0195560:CDS 30.0%
!! GTTTGTGGTTCTTTTTCATT+TGG + chr2_1:12391452-12391471 Msa0195560:CDS 30.0%
! ATCCTTCGATTTTCACATAA+AGG + chr2_1:12391559-12391578 Msa0195560:intron 30.0%
AAACATTTCAACCATGTGAT+TGG - chr2_1:12391711-12391730 None:intergenic 30.0%
AATTGGATAAGTGTTTGCAA+TGG + chr2_1:12391831-12391850 Msa0195560:intron 30.0%
ATTGGATAAGTGTTTGCAAT+GGG + chr2_1:12391832-12391851 Msa0195560:intron 30.0%
!!! AGGCTGTAAATATTTTTTGG+AGG - chr2_1:12391914-12391933 None:intergenic 30.0%
!!! ATACAATGTGTTTTGGAAAG+GGG - chr2_1:12392219-12392238 None:intergenic 30.0%
!!! CATACAATGTGTTTTGGAAA+GGG - chr2_1:12392220-12392239 None:intergenic 30.0%
!!! TCATACAATGTGTTTTGGAA+AGG - chr2_1:12392221-12392240 None:intergenic 30.0%
! CTCATTCATACAATGTGTTT+TGG - chr2_1:12392226-12392245 None:intergenic 30.0%
! CTAGTTTCTATTTTGTGATC+AGG + chr2_1:12392521-12392540 Msa0195560:intron 30.0%
! TTTCTATTTTGTGATCAGGT+GGG + chr2_1:12392525-12392544 Msa0195560:intron 30.0%
ATGAGTGTATCAAGAAGAAT+AGG + chr2_1:12392769-12392788 Msa0195560:intron 30.0%
AATACGTGTCATAGATTCAT+AGG - chr2_1:12393106-12393125 None:intergenic 30.0%
ATGAATCTATGACACGTATT+AGG + chr2_1:12393106-12393125 Msa0195560:intron 30.0%
TGTATATCACTATCCATTAC+CGG + chr2_1:12393240-12393259 Msa0195560:intron 30.0%
!!! ACATAATATGTTTTGGGGAT+TGG + chr2_1:12393451-12393470 Msa0195560:intron 30.0%
!!! GTTTTCAATGTTTGGTTCTT+TGG + chr2_1:12393476-12393495 Msa0195560:intron 30.0%
! TTTTTCACAGGATATGGAAT+TGG + chr2_1:12393844-12393863 Msa0195560:CDS 30.0%
AATTGGAGTTATCTCATATG+TGG + chr2_1:12393861-12393880 Msa0195560:CDS 30.0%
CATTCATCAAGTGATTTGAA+AGG - chr2_1:12394078-12394097 None:intergenic 30.0%
! TGGTTCTCTGAAAATGATTA+TGG - chr2_1:12394141-12394160 None:intergenic 30.0%
CAATCAGTATGTGTTTAGTT+TGG - chr2_1:12394197-12394216 None:intergenic 30.0%
!! GCTATTTCTGCTATATATAC+TGG - chr2_1:12394310-12394329 None:intergenic 30.0%
ATAGCACCAAAAAATCTTAG+AGG + chr2_1:12394325-12394344 Msa0195560:CDS 30.0%
TTGCAGCTTATGATTGTTAT+TGG + chr2_1:12394447-12394466 Msa0195560:intron 30.0%
!! ACTCTTGATTTGATTTTGCT+TGG + chr2_1:12394600-12394619 Msa0195560:intron 30.0%
AACTAGCATTAAGGAAACTA+CGG + chr2_1:12395020-12395039 Msa0195560:CDS 30.0%
TGATGAAGCTAATGAAATTC+TGG + chr2_1:12395063-12395082 Msa0195560:CDS 30.0%
CTTGATCAGTTAAGAAAGTT+TGG - chr2_1:12395095-12395114 None:intergenic 30.0%
AGCAAAATTGAGTTCAGATA+AGG - chr2_1:12395159-12395178 None:intergenic 30.0%
! CTGAACTCAATTTTGCTTAT+AGG + chr2_1:12395162-12395181 Msa0195560:intron 30.0%
CAACAATCTGTTGGAATTAA+TGG + chr2_1:12395383-12395402 Msa0195560:CDS 30.0%
CATAGTGCTATATTTAAGGA+CGG - chr2_1:12395516-12395535 None:intergenic 30.0%
CCGAAAATTGTAACATAATC+AGG + chr2_1:12395677-12395696 Msa0195560:intron 30.0%
ATTGAGGATGGAGAAACTAA+TGG + chr2_1:12391308-12391327 Msa0195560:CDS 35.0%
TTGAGGATGGAGAAACTAAT+GGG + chr2_1:12391309-12391328 Msa0195560:CDS 35.0%
CAAGAACCTTTCATTCAACA+AGG + chr2_1:12391344-12391363 Msa0195560:CDS 35.0%
ACCTTTCATTCAACAAGGAA+AGG + chr2_1:12391349-12391368 Msa0195560:CDS 35.0%
TCCTTTCCTTGTTGAATGAA+AGG - chr2_1:12391353-12391372 None:intergenic 35.0%
AAGGATGCTTGTAAAGAAGT+TGG + chr2_1:12391368-12391387 Msa0195560:CDS 35.0%
AGTTGGATCAGATAAAAGTG+TGG + chr2_1:12391385-12391404 Msa0195560:CDS 35.0%
GTCCTTTATGTGAAAATCGA+AGG - chr2_1:12391564-12391583 None:intergenic 35.0%
! GACTTTGATAACCTAGAGTT+CGG + chr2_1:12391581-12391600 Msa0195560:intron 35.0%
TTATCATCTTACCGAACTCT+AGG - chr2_1:12391595-12391614 None:intergenic 35.0%
ATAGGAACTGGTATGTTAGT+GGG - chr2_1:12391944-12391963 None:intergenic 35.0%
!!! TGTTGCTGTTTCGGTATTTT+GGG + chr2_1:12391984-12392003 Msa0195560:intron 35.0%
!! TCTTTTGGTGTATGGCTTTT+TGG + chr2_1:12392025-12392044 Msa0195560:intron 35.0%
AAAGGACACAGCAGAAAAAA+AGG - chr2_1:12392069-12392088 None:intergenic 35.0%
TACTATACCAAAGCAAACAG+CGG - chr2_1:12392093-12392112 None:intergenic 35.0%
! AATGAGCCTCATTTTACTTC+CGG + chr2_1:12392240-12392259 Msa0195560:intron 35.0%
AAACTAACACATGCAAACCA+GGG - chr2_1:12392294-12392313 None:intergenic 35.0%
TAAACTAACACATGCAAACC+AGG - chr2_1:12392295-12392314 None:intergenic 35.0%
! GTTTCTATTTTGTGATCAGG+TGG + chr2_1:12392524-12392543 Msa0195560:intron 35.0%
TCTTAATCTATCTCTTGCTG+AGG + chr2_1:12392584-12392603 Msa0195560:CDS 35.0%
GTATATCACTATCCATTACC+GGG + chr2_1:12393241-12393260 Msa0195560:intron 35.0%
TATATCACTATCCATTACCG+GGG + chr2_1:12393242-12393261 Msa0195560:intron 35.0%
ACAAATTCTGTACTTTACCC+CGG - chr2_1:12393262-12393281 None:intergenic 35.0%
! GATTGGCAGTTTTCAATGTT+TGG + chr2_1:12393468-12393487 Msa0195560:intron 35.0%
! TCCACAGGATTTTGTATCAT+AGG + chr2_1:12393670-12393689 Msa0195560:CDS 35.0%
TCCTATGATACAAAATCCTG+TGG - chr2_1:12393674-12393693 None:intergenic 35.0%
! CAGGATTTTGTATCATAGGA+TGG + chr2_1:12393674-12393693 Msa0195560:CDS 35.0%
AGTTCTTACACACTTGACTT+GGG + chr2_1:12393817-12393836 Msa0195560:CDS 35.0%
!! TGCATGCAGTGTTACTTAAT+TGG + chr2_1:12394215-12394234 Msa0195560:intron 35.0%
!! AAAAATCAGCATAACGGATC+AGG + chr2_1:12394282-12394301 Msa0195560:intron 35.0%
!!! AGTCCTCCTCTAAGATTTTT+TGG - chr2_1:12394334-12394353 None:intergenic 35.0%
!! TTGATTTGATTTTGCTTGGC+TGG + chr2_1:12394604-12394623 Msa0195560:intron 35.0%
!!! TGCACAATTTTTTCAGGCAA+AGG + chr2_1:12394976-12394995 Msa0195560:intron 35.0%
!!! CAATTTTTTCAGGCAAAGGT+TGG + chr2_1:12394980-12394999 Msa0195560:intron 35.0%
ACTAGCATTAAGGAAACTAC+GGG + chr2_1:12395021-12395040 Msa0195560:CDS 35.0%
!! CCAAAAACTTAGTCTTAGGA+AGG - chr2_1:12395210-12395229 None:intergenic 35.0%
! CCTTCCTAAGACTAAGTTTT+TGG + chr2_1:12395207-12395226 Msa0195560:CDS 35.0%
GAACAATTTCAGATTGGAGT+TGG + chr2_1:12395347-12395366 Msa0195560:intron 35.0%
TCAGATTGGAGTTGGATTAA+TGG + chr2_1:12395355-12395374 Msa0195560:intron 35.0%
ATGGCATTTCAACAATCTGT+TGG + chr2_1:12395374-12395393 Msa0195560:CDS 35.0%
TCTGTTGGAATTAATGGCAT+AGG + chr2_1:12395389-12395408 Msa0195560:CDS 35.0%
! AATGAATTGCATGCTGATTG+TGG + chr2_1:12395452-12395471 Msa0195560:intron 35.0%
TGTCAGAACATTTACACATG+AGG - chr2_1:12395935-12395954 None:intergenic 35.0%
TCATGTGTAAATGTTCTGAC+AGG + chr2_1:12395934-12395953 Msa0195560:intron 35.0%
CGAACTATCAACAAATTCTG+TGG + chr2_1:12396012-12396031 Msa0195560:CDS 35.0%
TGAGCAGCATAAGGATATTG+AGG + chr2_1:12391292-12391311 Msa0195560:CDS 40.0%
CAGCATAAGGATATTGAGGA+TGG + chr2_1:12391296-12391315 Msa0195560:CDS 40.0%
AGTGTGGAAAATGGATCCAT+TGG + chr2_1:12391401-12391420 Msa0195560:CDS 40.0%
GGAAAATGGATCCATTGGAA+TGG + chr2_1:12391406-12391425 Msa0195560:CDS 40.0%
!! AGCACATTTGTTGCTGTTTG+TGG + chr2_1:12391437-12391456 Msa0195560:CDS 40.0%
TTCAACCATGTGATTGGACT+CGG - chr2_1:12391705-12391724 None:intergenic 40.0%
TAGGAACTGGTATGTTAGTG+GGG - chr2_1:12391943-12391962 None:intergenic 40.0%
CATAGGAACTGGTATGTTAG+TGG - chr2_1:12391945-12391964 None:intergenic 40.0%
AAAGCATGCTACAACAGCAT+AGG - chr2_1:12391962-12391981 None:intergenic 40.0%
!! ATGCTTTGCTGTTGCTGTTT+CGG + chr2_1:12391975-12391994 Msa0195560:intron 40.0%
!!! CTGTTGCTGTTTCGGTATTT+TGG + chr2_1:12391983-12392002 Msa0195560:intron 40.0%
! TTTGGTTTCTTTCCTGTTGC+TGG + chr2_1:12392043-12392062 Msa0195560:intron 40.0%
GCTGTTTGCTTTGGTATAGT+AGG + chr2_1:12392092-12392111 Msa0195560:intron 40.0%
TTTGCTTTGGTATAGTAGGC+TGG + chr2_1:12392096-12392115 Msa0195560:intron 40.0%
TTGCTTTGGTATAGTAGGCT+GGG + chr2_1:12392097-12392116 Msa0195560:intron 40.0%
!! TTTGGTTCTTTGGTGACCAT+TGG + chr2_1:12393486-12393505 Msa0195560:CDS 40.0%
ATGCTTGGAGCTATAACAAG+TGG + chr2_1:12393513-12393532 Msa0195560:CDS 40.0%
GGTCGAGTTACTGATATCAT+TGG + chr2_1:12393534-12393553 Msa0195560:CDS 40.0%
GAGTTACTGATATCATTGGC+AGG + chr2_1:12393538-12393557 Msa0195560:CDS 40.0%
ACTGATATCATTGGCAGGAA+AGG + chr2_1:12393543-12393562 Msa0195560:CDS 40.0%
CTGATATCATTGGCAGGAAA+GGG + chr2_1:12393544-12393563 Msa0195560:CDS 40.0%
TGATATCATTGGCAGGAAAG+GGG + chr2_1:12393545-12393564 Msa0195560:CDS 40.0%
GAGTTCTTACACACTTGACT+TGG + chr2_1:12393816-12393835 Msa0195560:intron 40.0%
! GGAAGGTTTTTCACAGGATA+TGG + chr2_1:12393838-12393857 Msa0195560:CDS 40.0%
GCACCAAAAAATCTTAGAGG+AGG + chr2_1:12394328-12394347 Msa0195560:CDS 40.0%
AGGACTTGCAACAACAAATC+AGG + chr2_1:12394348-12394367 Msa0195560:CDS 40.0%
TTGCAACAACAAATCAGGTG+AGG + chr2_1:12394353-12394372 Msa0195560:intron 40.0%
CTAGCATTAAGGAAACTACG+GGG + chr2_1:12395022-12395041 Msa0195560:CDS 40.0%
!!! TGCAGATTTTGGATTGGCTA+GGG - chr2_1:12395971-12395990 None:intergenic 40.0%
!!! TTGCAGATTTTGGATTGGCT+AGG - chr2_1:12395972-12395991 None:intergenic 40.0%
! AAGGCTTGCAGATTTTGGAT+TGG - chr2_1:12395977-12395996 None:intergenic 40.0%
! TCAGTAAGGCTTGCAGATTT+TGG - chr2_1:12395982-12396001 None:intergenic 40.0%
CAAGCCTTACTGAATTAGTG+CGG + chr2_1:12395989-12396008 Msa0195560:CDS 40.0%
GCTAAGCAGAACCATTCCAA+TGG - chr2_1:12391420-12391439 None:intergenic 45.0%
GATAAGTGTTTGCAATGGGC+CGG + chr2_1:12391836-12391855 Msa0195560:intron 45.0%
AGGAACTGGTATGTTAGTGG+GGG - chr2_1:12391942-12391961 None:intergenic 45.0%
TGCTACAACAGCATAGGAAC+TGG - chr2_1:12391956-12391975 None:intergenic 45.0%
!!! TGCTGTTTCGGTATTTTGGG+AGG + chr2_1:12391987-12392006 Msa0195560:intron 45.0%
!! GCTGTTTCGGTATTTTGGGA+GGG + chr2_1:12391988-12392007 Msa0195560:intron 45.0%
CAGAAAAAAAGGCCAGCAAC+AGG - chr2_1:12392058-12392077 None:intergenic 45.0%
! TTTGCCATTTAGTGCACTCC+TGG + chr2_1:12392145-12392164 Msa0195560:intron 45.0%
! TATTTCAAGTGCCAAGCACC+AGG - chr2_1:12392166-12392185 None:intergenic 45.0%
TTCCAACGAATTTGGAGGCT+CGG - chr2_1:12392268-12392287 None:intergenic 45.0%
AGCCTCCAAATTCGTTGGAA+CGG + chr2_1:12392268-12392287 Msa0195560:intron 45.0%
TTATTCAGCACCCACTCAAG+CGG + chr2_1:12392548-12392567 Msa0195560:CDS 45.0%
ATAGGCTAGACAGACTCACA+TGG + chr2_1:12392787-12392806 Msa0195560:intron 45.0%
TCTGTACTTTACCCCGGTAA+TGG - chr2_1:12393256-12393275 None:intergenic 45.0%
CAGGCTATGAGAATTTCCAC+AGG + chr2_1:12393655-12393674 Msa0195560:intron 45.0%
GCTAGCTGTCTTCTTCTCTA+AGG + chr2_1:12393696-12393715 Msa0195560:CDS 45.0%
!! CTTACACACTTGACTTGGGA+AGG + chr2_1:12393821-12393840 Msa0195560:CDS 45.0%
! GACTTGGGAAGGTTTTTCAC+AGG + chr2_1:12393832-12393851 Msa0195560:CDS 45.0%
! TGCTTGGCTGGAACTTTTGA+AGG + chr2_1:12394616-12394635 Msa0195560:intron 45.0%
GCAAGCAAATGCAAGGAACT+AGG - chr2_1:12394642-12394661 None:intergenic 45.0%
CCAACAAGCAAGCAAATGCA+AGG - chr2_1:12394649-12394668 None:intergenic 45.0%
CCTTGCATTTGCTTGCTTGT+TGG + chr2_1:12394646-12394665 Msa0195560:CDS 45.0%
TCATTCCTGAGTCTCCTAGA+TGG + chr2_1:12394677-12394696 Msa0195560:CDS 45.0%
CAGAGCGCACATATTTGCTT+TGG - chr2_1:12395240-12395259 None:intergenic 45.0%
!! TTTGGATTGGCTAGGGCATT+TGG - chr2_1:12395964-12395983 None:intergenic 45.0%
TTCGCCGCACTAATTCAGTA+AGG - chr2_1:12395996-12396015 None:intergenic 45.0%
!!! TTTTATTTTATAGAAAAAAA+AGG - chr2_1:12392678-12392697 None:intergenic 5.0%
AGCATCCGAGTCCAATCACA+TGG + chr2_1:12391697-12391716 Msa0195560:intron 50.0%
!! GTATGTTAGTGGGGGGTACT+AGG - chr2_1:12391934-12391953 None:intergenic 50.0%
GGAACTGGTATGTTAGTGGG+GGG - chr2_1:12391941-12391960 None:intergenic 50.0%
TCCTTTGCCGCTGTTTGCTT+TGG + chr2_1:12392083-12392102 Msa0195560:intron 50.0%
ACCAAAGCAAACAGCGGCAA+AGG - chr2_1:12392087-12392106 None:intergenic 50.0%
! AGCACCAGGAGTGCACTAAA+TGG - chr2_1:12392152-12392171 None:intergenic 50.0%
TTTAGTGCACTCCTGGTGCT+TGG + chr2_1:12392152-12392171 Msa0195560:intron 50.0%
!! TCGGTGCCGGAAGTAAAATG+AGG - chr2_1:12392249-12392268 None:intergenic 50.0%
GTGACCATTGGTGCAATGCT+TGG + chr2_1:12393498-12393517 Msa0195560:CDS 50.0%
! AGCTCCAAGCATTGCACCAA+TGG - chr2_1:12393505-12393524 None:intergenic 50.0%
AGTCTCCTAGATGGCTGGTA+AGG + chr2_1:12394686-12394705 Msa0195560:intron 50.0%
TCCTAGATGGCTGGTAAGGA+AGG + chr2_1:12394690-12394709 Msa0195560:intron 50.0%
TCCTTCCTTACCAGCCATCT+AGG - chr2_1:12394694-12394713 None:intergenic 50.0%
! GCTGAGACTTTTGTAGCAGC+TGG + chr2_1:12395422-12395441 Msa0195560:CDS 50.0%
GCAAGCAGTGCTATAGCACC+AGG + chr2_1:12391871-12391890 Msa0195560:intron 55.0%
!! TTTTTGGAGGCCCGTGCCTT+GGG - chr2_1:12391901-12391920 None:intergenic 55.0%
!!! TTTTTTGGAGGCCCGTGCCT+TGG - chr2_1:12391902-12391921 None:intergenic 55.0%
CACCGAGCCTCCAAATTCGT+TGG + chr2_1:12392263-12392282 Msa0195560:intron 55.0%
GGCCGTTCCAACGAATTTGG+AGG - chr2_1:12392273-12392292 None:intergenic 55.0%
CAGGGCCGTTCCAACGAATT+TGG - chr2_1:12392276-12392295 None:intergenic 55.0%
CAAATTCGTTGGAACGGCCC+TGG + chr2_1:12392274-12392293 Msa0195560:intron 55.0%
GACCTGATAGCCGCTTGAGT+GGG - chr2_1:12392561-12392580 None:intergenic 55.0%
AGACCTGATAGCCGCTTGAG+TGG - chr2_1:12392562-12392581 None:intergenic 55.0%
!! TGGCGACAACTAGCTCTAGC+AGG + chr2_1:12394507-12394526 Msa0195560:CDS 55.0%
TCCTGAGTCTCCTAGATGGC+TGG + chr2_1:12394681-12394700 Msa0195560:CDS 55.0%
ACCAGCCATCTAGGAGACTC+AGG - chr2_1:12394685-12394704 None:intergenic 55.0%
TTTGCAATGGGCCGGACCCT+GGG + chr2_1:12391844-12391863 Msa0195560:intron 60.0%
TATAGCACCAGGCCTCCCCA+AGG + chr2_1:12391882-12391901 Msa0195560:intron 60.0%
!! TTTTGGAGGCCCGTGCCTTG+GGG - chr2_1:12391900-12391919 None:intergenic 60.0%
!! CGAATTTGGAGGCTCGGTGC+CGG - chr2_1:12392262-12392281 None:intergenic 60.0%
CACCCACTCAAGCGGCTATC+AGG + chr2_1:12392556-12392575 Msa0195560:CDS 60.0%
GTTTGCAATGGGCCGGACCC+TGG + chr2_1:12391843-12391862 Msa0195560:intron 65.0%
TTGCAATGGGCCGGACCCTG+GGG + chr2_1:12391845-12391864 Msa0195560:intron 65.0%
AGCACTGCTTGCACCCCCCA+GGG - chr2_1:12391863-12391882 None:intergenic 65.0%
TAGCACTGCTTGCACCCCCC+AGG - chr2_1:12391864-12391883 None:intergenic 65.0%
TGCAATGGGCCGGACCCTGG+GGG + chr2_1:12391846-12391865 Msa0195560:intron 70.0%
TGCTTGCACCCCCCAGGGTC+CGG - chr2_1:12391858-12391877 None:intergenic 70.0%
CACCAGGCCTCCCCAAGGCA+CGG + chr2_1:12391887-12391906 Msa0195560:intron 70.0%
ACCAGGCCTCCCCAAGGCAC+GGG + chr2_1:12391888-12391907 Msa0195560:intron 70.0%
GCAATGGGCCGGACCCTGGG+GGG + chr2_1:12391847-12391866 Msa0195560:intron 75.0%
TGGAGGCCCGTGCCTTGGGG+AGG - chr2_1:12391897-12391916 None:intergenic 75.0%
! GCCCGTGCCTTGGGGAGGCC+TGG - chr2_1:12391892-12391911 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 12391284 12396050 12391284 ID=Msa0195560;Name=Msa0195560
chr2_1 mRNA 12391284 12396050 12391284 ID=Msa0195560-mRNA-1;Parent=Msa0195560;Name=Msa0195560-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0.36|0.25|0.91|1|1|12|0|345
chr2_1 exon 12391284 12391481 12391284 ID=Msa0195560-mRNA-1:exon:5416;Parent=Msa0195560-mRNA-1
chr2_1 exon 12392543 12392605 12392543 ID=Msa0195560-mRNA-1:exon:5417;Parent=Msa0195560-mRNA-1
chr2_1 exon 12393477 12393566 12393477 ID=Msa0195560-mRNA-1:exon:5418;Parent=Msa0195560-mRNA-1
chr2_1 exon 12393658 12393717 12393658 ID=Msa0195560-mRNA-1:exon:5419;Parent=Msa0195560-mRNA-1
chr2_1 exon 12393817 12393882 12393817 ID=Msa0195560-mRNA-1:exon:5420;Parent=Msa0195560-mRNA-1
chr2_1 exon 12394304 12394369 12394304 ID=Msa0195560-mRNA-1:exon:5421;Parent=Msa0195560-mRNA-1
chr2_1 exon 12394453 12394528 12394453 ID=Msa0195560-mRNA-1:exon:5422;Parent=Msa0195560-mRNA-1
chr2_1 exon 12394638 12394702 12394638 ID=Msa0195560-mRNA-1:exon:5423;Parent=Msa0195560-mRNA-1
chr2_1 exon 12394992 12395084 12394992 ID=Msa0195560-mRNA-1:exon:5424;Parent=Msa0195560-mRNA-1
chr2_1 exon 12395184 12395264 12395184 ID=Msa0195560-mRNA-1:exon:5425;Parent=Msa0195560-mRNA-1
chr2_1 exon 12395359 12395443 12395359 ID=Msa0195560-mRNA-1:exon:5426;Parent=Msa0195560-mRNA-1
chr2_1 exon 12395956 12396050 12395956 ID=Msa0195560-mRNA-1:exon:5427;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12391284 12391481 12391284 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12392543 12392605 12392543 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12393477 12393566 12393477 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12393658 12393717 12393658 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12393817 12393882 12393817 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12394304 12394369 12394304 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12394453 12394528 12394453 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12394638 12394702 12394638 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12394992 12395084 12394992 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12395184 12395264 12395184 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12395359 12395443 12395359 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
chr2_1 CDS 12395956 12396050 12395956 ID=Msa0195560-mRNA-1:cds;Parent=Msa0195560-mRNA-1
Gene Sequence

>Msa0195560

ATGACAATTGAGCAGCATAAGGATATTGAGGATGGAGAAACTAATGGGTTTCAGTATTTGCAAGAACCTTTCATTCAACAAGGAAAGGATGCTTGTAAAGAAGTTGGATCAGATAAAAGTGTGGAAAATGGATCCATTGGAATGGTTCTGCTTAGCACATTTGTTGCTGTTTGTGGTTCTTTTTCATTTGGAACTTGTGTGGGTTATTCAGCACCCACTCAAGCGGCTATCAGGTCTGATCTTAATCTATCTCTTGCTGAGTTTTCAATGTTTGGTTCTTTGGTGACCATTGGTGCAATGCTTGGAGCTATAACAAGTGGTCGAGTTACTGATATCATTGGCAGGAAAGGGGCTATGAGAATTTCCACAGGATTTTGTATCATAGGATGGCTAGCTGTCTTCTTCTCTAAGAGTTCTTACACACTTGACTTGGGAAGGTTTTTCACAGGATATGGAATTGGAGTTATCTCATATGTGGTTCCAGTATATATAGCAGAAATAGCACCAAAAAATCTTAGAGGAGGACTTGCAACAACAAATCAGCTTATGATTGTTATTGGATCATCAATGTCATTTTTAATAGGAAGTCTCATAAATTGGCGACAACTAGCTCTAGCAGGCCTAGTTCCTTGCATTTGCTTGCTTGTTGGTCTGTGCTTCATTCCTGAGTCTCCTAGATGGCTGGCAAAGGTTGGTCGTGAAAAAGAATTTCAACTAGCATTAAGGAAACTACGGGGTAAAGACGTTGATATTTCTGATGAAGCTAATGAAATTCTGGATAATATTGAAACTCTTCAAAGCCTTCCTAAGACTAAGTTTTTGGATTTGTTCCAAAGCAAATATGTGCGCTCTGTAGTTATTGGAGTTGGATTAATGGCATTTCAACAATCTGTTGGAATTAATGGCATAGGATTCTATACAGCTGAGACTTTTGTAGCAGCTGGTATGCCAAATGCCCTAGCCAATCCAAAATCTGCAAGCCTTACTGAATTAGTGCGGCGAACTATCAACAAATTCTGTGGTTTCAAGTCTCAATGA
Protein sequence

>Msa0195560

MTIEQHKDIEDGETNGFQYLQEPFIQQGKDACKEVGSDKSVENGSIGMVLLSTFVAVCGSFSFGTCVGYSAPTQAAIRSDLNLSLAEFSMFGSLVTIGAMLGAITSGRVTDIIGRKGAMRISTGFCIIGWLAVFFSKSSYTLDLGRFFTGYGIGVISYVVPVYIAEIAPKNLRGGLATTNQLMIVIGSSMSFLIGSLINWRQLALAGLVPCICLLVGLCFIPESPRWLAKVGREKEFQLALRKLRGKDVDISDEANEILDNIETLQSLPKTKFLDLFQSKYVRSVVIGVGLMAFQQSVGINGIGFYTAETFVAAGMPNALANPKSASLTELVRRTINKFCGFKSQ*