AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0223270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0223270 MtrunA17_Chr8g0366381 100.000 80 0 0 1 80 1 80 1.39e-53 160
Msa0223270 MtrunA17_Chr2g0282641 100.000 78 0 0 1 78 1 78 4.79e-52 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0223270 AT1G74340.1 82.500 80 14 0 1 80 1 80 3.52e-38 122
Msa0223270 AT1G74340.2 82.500 80 14 0 1 80 1 80 3.52e-38 122

Find 52 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTAGTAGTTTCGCTATT+TGG 0.167609 2_1:-72807408 None:intergenic
TGCCTCTTATGCATATTTAT+TGG 0.211849 2_1:+72810237 Msa0223270:CDS
ACATTATGCCTTCTTCTTCT+TGG 0.225894 2_1:-72810277 None:intergenic
TATTCACTTATTATACGTTT+TGG 0.247618 2_1:+72810024 Msa0223270:CDS
GCCTCTTATGCATATTTATT+GGG 0.258483 2_1:+72810238 Msa0223270:CDS
GGATTAATATGGCATATTCT+TGG 0.312708 2_1:-72810188 None:intergenic
AATTAGTAGTTTCGCTATTT+GGG 0.323295 2_1:-72807407 None:intergenic
GATTAATATGGCATATTCTT+GGG 0.324167 2_1:-72810187 None:intergenic
TACTACGTTCCAAGTAAAAC+GGG 0.363183 2_1:-72810744 None:intergenic
TACAGCACACAACAGGGATT+TGG 0.372617 2_1:+72810462 Msa0223270:three_prime_UTR
TAATTTACCCTTGATTGATC+AGG 0.381535 2_1:+72807425 Msa0223270:five_prime_UTR
AGAGCAACACCAGCAGAAAC+AGG 0.394145 2_1:-72810210 None:intergenic
TAGCATCTACAGCACACAAC+AGG 0.400595 2_1:+72810455 Msa0223270:three_prime_UTR
ACCCAATAAATATGCATAAG+AGG 0.411050 2_1:-72810239 None:intergenic
GAATATCGATAAGCTTATAA+AGG 0.417351 2_1:-72810006 None:intergenic
AGCAGAAACAGGGATTAATA+TGG 0.417601 2_1:-72810199 None:intergenic
ATATTAATCCCTGTTTCTGC+TGG 0.419614 2_1:+72810201 Msa0223270:CDS
ATGACCCTAGTGTCATGAAT+TGG 0.435781 2_1:+72810379 Msa0223270:three_prime_UTR
TTCAAAATCTCACGAGTTGT+TGG 0.445513 2_1:-72809791 None:intergenic
CAAAATTGTTTGTGTCGCCT+TGG 0.447357 2_1:+72810593 Msa0223270:three_prime_UTR
ATACTACGTTCCAAGTAAAA+CGG 0.452510 2_1:-72810745 None:intergenic
CTCCTTACATGAGGGTGAAA+AGG 0.454533 2_1:+72810616 Msa0223270:three_prime_UTR
AATTCAATAAGCAAAATGTT+TGG 0.456319 2_1:-72810668 None:intergenic
GAATTAGCAGACAGAGCAGT+TGG 0.476638 2_1:+72809972 Msa0223270:CDS
AATGAGAAAATGAGAGGAAA+CGG 0.478007 2_1:-72810492 None:intergenic
TTTGCTTATTGAATTCTCCT+TGG 0.478047 2_1:+72810676 Msa0223270:three_prime_UTR
TTCTGAATCCTGATCAATCA+AGG 0.479359 2_1:-72807433 None:intergenic
GTGCTGTAGATGCTACATTA+AGG 0.481697 2_1:-72810447 None:intergenic
AAATGATCATCATCTACAAA+GGG 0.512690 2_1:-72810144 None:intergenic
CACCCTCATGTAAGGAGCCA+AGG 0.527336 2_1:-72810610 None:intergenic
ATTAATATGGCATATTCTTG+GGG 0.547808 2_1:-72810186 None:intergenic
TCATCGTCTTCCGTCGTGAA+CGG 0.552178 2_1:+72807541 Msa0223270:five_prime_UTR
CTTTATACGATCGGTTGAGA+AGG 0.560463 2_1:-72807488 None:intergenic
CGCCTTGGCTCCTTACATGA+GGG 0.560778 2_1:+72810608 Msa0223270:three_prime_UTR
GGTAGCCAATTCATGACACT+AGG 0.578234 2_1:-72810384 None:intergenic
AACAAGAATGAGAAAATGAG+AGG 0.579795 2_1:-72810498 None:intergenic
CACTTTAAAAGGTGATACCA+AGG 0.582597 2_1:-72810693 None:intergenic
CGTATAAAGCAAAAGTTCAC+CGG 0.582917 2_1:+72807502 Msa0223270:five_prime_UTR
AAAGTCTGCAGTGAGCTGAA+AGG 0.583146 2_1:-72809816 None:intergenic
GAAATGATCATCATCTACAA+AGG 0.583886 2_1:-72810145 None:intergenic
TCGCCTTGGCTCCTTACATG+AGG 0.583973 2_1:+72810607 Msa0223270:three_prime_UTR
GCTGAAATCCAAGAAGAAGA+AGG 0.589308 2_1:+72810269 Msa0223270:CDS
CGACGGAAGACGATGAACTC+CGG 0.618357 2_1:-72807534 None:intergenic
TCTGAATCCTGATCAATCAA+GGG 0.622653 2_1:-72807432 None:intergenic
AGCATCTACAGCACACAACA+GGG 0.640548 2_1:+72810456 Msa0223270:three_prime_UTR
TACATGAGGGTGAAAAGGAG+AGG 0.641050 2_1:+72810621 Msa0223270:three_prime_UTR
GAGCAACACCAGCAGAAACA+GGG 0.653237 2_1:-72810209 None:intergenic
GTAGCCAATTCATGACACTA+GGG 0.661120 2_1:-72810383 None:intergenic
CGGAAGACGATGAACTCCGG+CGG 0.690775 2_1:-72807531 None:intergenic
ATGTTACTTACCGTTCACGA+CGG 0.713826 2_1:-72807551 None:intergenic
GAAAATGAGAGGAAACGGAG+AGG 0.720951 2_1:-72810487 None:intergenic
ACTACGTTCCAAGTAAAACG+GGG 0.754902 2_1:-72810743 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAAAATTAGATATTAA+CGG + chr2_1:72807828-72807847 Msa0223270:intron 10.0%
!!! AAATTAGTTTAATTATGAAT+TGG + chr2_1:72807864-72807883 Msa0223270:intron 10.0%
!! GTAAATTAAAATCAATATAT+TGG + chr2_1:72808618-72808637 Msa0223270:intron 10.0%
!! ATATAATAAACAATACTTTA+CGG - chr2_1:72808823-72808842 None:intergenic 10.0%
!!! TAATATTCTAGAAAATTATA+CGG + chr2_1:72809364-72809383 Msa0223270:intron 10.0%
!!! ATTTGATGTAATTTTTTATA+AGG + chr2_1:72809604-72809623 Msa0223270:intron 10.0%
!! TCATATTTATTATTCAAAAA+AGG - chr2_1:72809648-72809667 None:intergenic 10.0%
!! TGATAAAATTAGATATTAAC+GGG + chr2_1:72807829-72807848 Msa0223270:intron 15.0%
!!! AATGATATATTTTAGTTTGA+TGG + chr2_1:72808726-72808745 Msa0223270:intron 15.0%
!! TATAATAAACAATACTTTAC+GGG - chr2_1:72808822-72808841 None:intergenic 15.0%
!! ATTATTATTTGACTAATCAA+TGG + chr2_1:72808998-72809017 Msa0223270:intron 15.0%
!! AATATGATTAGTAAAGTAAA+GGG + chr2_1:72809022-72809041 Msa0223270:intron 15.0%
!!! AATATTCTAGAAAATTATAC+GGG + chr2_1:72809365-72809384 Msa0223270:intron 15.0%
!! TTGAATAAAAACCATAAAAT+AGG - chr2_1:72809532-72809551 None:intergenic 15.0%
!! AAACAAATTCAACAAAATAT+TGG - chr2_1:72809869-72809888 None:intergenic 15.0%
!!! CATAATACATTTAAAATACT+TGG - chr2_1:72810074-72810093 None:intergenic 15.0%
!!! CAAGTATTTTAAATGTATTA+TGG + chr2_1:72810072-72810091 Msa0223270:intron 15.0%
!!! TATTTTGATTTCACTTTAAA+AGG - chr2_1:72810707-72810726 None:intergenic 15.0%
!! TATTACTCAATTTGATTGAA+AGG - chr2_1:72807698-72807717 None:intergenic 20.0%
!! AGCCCATTTATTAAAATTAA+GGG - chr2_1:72807934-72807953 None:intergenic 20.0%
!! AGTTAAGCTAACATATATAA+GGG - chr2_1:72807992-72808011 None:intergenic 20.0%
!! ATGAATTAGATAAACAATGT+TGG + chr2_1:72808013-72808032 Msa0223270:intron 20.0%
!! AGAGAAATATAAAAGATTCA+AGG - chr2_1:72808056-72808075 None:intergenic 20.0%
!!! TTGAATCTTTTATATTTCTC+TGG + chr2_1:72808055-72808074 Msa0223270:intron 20.0%
!! TCTTTATAATAGAGATCAAT+TGG + chr2_1:72808142-72808161 Msa0223270:intron 20.0%
!! TGTTAATAGAGTGAAAAATA+AGG - chr2_1:72808328-72808347 None:intergenic 20.0%
!! TTATGGAAATATTAGCTATT+TGG - chr2_1:72808426-72808445 None:intergenic 20.0%
!! TTATGTTCTGTTTAGTATAT+GGG + chr2_1:72808523-72808542 Msa0223270:intron 20.0%
!! TTAAGGATGAATTATATTAC+CGG + chr2_1:72808560-72808579 Msa0223270:intron 20.0%
!! TAAAATCAATATATTGGTCA+TGG + chr2_1:72808624-72808643 Msa0223270:intron 20.0%
!! ATAAACATACTTAAGAAACT+GGG - chr2_1:72808684-72808703 None:intergenic 20.0%
!! AATAAACATACTTAAGAAAC+TGG - chr2_1:72808685-72808704 None:intergenic 20.0%
!! TAAGTACACTTTAATAAGAT+AGG - chr2_1:72808760-72808779 None:intergenic 20.0%
!! CAATATGATTAGTAAAGTAA+AGG + chr2_1:72809021-72809040 Msa0223270:intron 20.0%
!! ATATGATTAGTAAAGTAAAG+GGG + chr2_1:72809023-72809042 Msa0223270:intron 20.0%
!!! ATATCATTTTCCTGAATAAT+TGG - chr2_1:72809067-72809086 None:intergenic 20.0%
!! TGTCTAAAAATCAAAAGAAT+GGG - chr2_1:72809151-72809170 None:intergenic 20.0%
!!! ATATTCTAGAAAATTATACG+GGG + chr2_1:72809366-72809385 Msa0223270:intron 20.0%
!! TTTAACAAAGAAAACTAAAC+CGG - chr2_1:72809448-72809467 None:intergenic 20.0%
!!! GATGTAATTTTTTATAAGGA+AGG + chr2_1:72809608-72809627 Msa0223270:intron 20.0%
!! AAAAACAATAGGAATCAAAA+TGG + chr2_1:72809950-72809969 Msa0223270:exon 20.0%
!!! TATTCACTTATTATACGTTT+TGG + chr2_1:72810024-72810043 Msa0223270:CDS 20.0%
!!! ATTCACTTATTATACGTTTT+GGG + chr2_1:72810025-72810044 Msa0223270:CDS 20.0%
!! AATTCAATAAGCAAAATGTT+TGG - chr2_1:72810671-72810690 None:intergenic 20.0%
! AATTAGTAGTTTCGCTATTT+GGG - chr2_1:72807410-72807429 None:intergenic 25.0%
! AAATTAGTAGTTTCGCTATT+TGG - chr2_1:72807411-72807430 None:intergenic 25.0%
! AAACTAATCGAGGAAATAAT+AGG - chr2_1:72807593-72807612 None:intergenic 25.0%
! ATTTCAATACAAACTAATCG+AGG - chr2_1:72807603-72807622 None:intergenic 25.0%
! TTTGATTGAAAGGTGAATTT+GGG - chr2_1:72807688-72807707 None:intergenic 25.0%
! ATTTGATTGAAAGGTGAATT+TGG - chr2_1:72807689-72807708 None:intergenic 25.0%
! CCCATTTATTAAAATTAAGG+GGG - chr2_1:72807932-72807951 None:intergenic 25.0%
!!! CCCCCTTAATTTTAATAAAT+GGG + chr2_1:72807929-72807948 Msa0223270:intron 25.0%
! GCCCATTTATTAAAATTAAG+GGG - chr2_1:72807933-72807952 None:intergenic 25.0%
! CAGCCCATTTATTAAAATTA+AGG - chr2_1:72807935-72807954 None:intergenic 25.0%
! GAGTTAAGCTAACATATATA+AGG - chr2_1:72807993-72808012 None:intergenic 25.0%
!!! CATTTCAAGTATTTTGACTT+TGG + chr2_1:72808346-72808365 Msa0223270:intron 25.0%
!! ATGATTCTTTTCTCCTTAAT+TGG + chr2_1:72808479-72808498 Msa0223270:intron 25.0%
!! TGATTCTTTTCTCCTTAATT+GGG + chr2_1:72808480-72808499 Msa0223270:intron 25.0%
! CTTATGTTCTGTTTAGTATA+TGG + chr2_1:72808522-72808541 Msa0223270:intron 25.0%
! TATGTTCTGTTTAGTATATG+GGG + chr2_1:72808524-72808543 Msa0223270:intron 25.0%
! TTATTATTAACCGTAGTCAA+CGG - chr2_1:72808785-72808804 None:intergenic 25.0%
! TATTATATCTAACGCTTTAC+CGG + chr2_1:72808835-72808854 Msa0223270:intron 25.0%
! AAGTTCTTAAATGACAGTAA+AGG - chr2_1:72808944-72808963 None:intergenic 25.0%
! TCTAAAAATCAAAAGAATGG+GGG - chr2_1:72809149-72809168 None:intergenic 25.0%
! GTCTAAAAATCAAAAGAATG+GGG - chr2_1:72809150-72809169 None:intergenic 25.0%
! CTGTCTAAAAATCAAAAGAA+TGG - chr2_1:72809152-72809171 None:intergenic 25.0%
! AGAATATTACAACTTGATAC+TGG - chr2_1:72809341-72809360 None:intergenic 25.0%
!! TTCTGTCAAAACCTATTTTA+TGG + chr2_1:72809518-72809537 Msa0223270:intron 25.0%
! ACTTGATTATAGCAAGAAAA+GGG - chr2_1:72809718-72809737 None:intergenic 25.0%
!! TGCAGACTTTAAAAATATAC+TGG + chr2_1:72809829-72809848 Msa0223270:five_prime_UTR 25.0%
! GAATATCGATAAGCTTATAA+AGG - chr2_1:72810009-72810028 None:intergenic 25.0%
! CACAGTACAATAAACTTATT+AGG - chr2_1:72810115-72810134 None:intergenic 25.0%
! AAATGATCATCATCTACAAA+GGG - chr2_1:72810147-72810166 None:intergenic 25.0%
! ATTAATATGGCATATTCTTG+GGG - chr2_1:72810189-72810208 None:intergenic 25.0%
! GATTAATATGGCATATTCTT+GGG - chr2_1:72810190-72810209 None:intergenic 25.0%
!!! CTAAATTTTGTTTGTGTGTA+AGG + chr2_1:72810565-72810584 Msa0223270:three_prime_UTR 25.0%
TAATTTACCCTTGATTGATC+AGG + chr2_1:72807425-72807444 Msa0223270:five_prime_UTR 30.0%
! GAACTTTTGCTTTATACGAT+CGG - chr2_1:72807500-72807519 None:intergenic 30.0%
AATCGAGGAAATAATAGGAA+AGG - chr2_1:72807588-72807607 None:intergenic 30.0%
AACTGTAAAATGAGATTCGA+TGG - chr2_1:72807630-72807649 None:intergenic 30.0%
AAAGGTGAATTTGGGTAAAA+GGG - chr2_1:72807680-72807699 None:intergenic 30.0%
ATGTAGAATTCGATAGATGT+TGG + chr2_1:72807728-72807747 Msa0223270:intron 30.0%
TGTAGAATTCGATAGATGTT+GGG + chr2_1:72807729-72807748 Msa0223270:intron 30.0%
!! GCCCCCTTAATTTTAATAAA+TGG + chr2_1:72807928-72807947 Msa0223270:intron 30.0%
TATAAAGAAACATCAGCAGT+AGG - chr2_1:72808130-72808149 None:intergenic 30.0%
ACTCACGTTTGTAATCAAAT+TGG + chr2_1:72808202-72808221 Msa0223270:intron 30.0%
CATTACTAAAATGAGGATTG+AGG - chr2_1:72808241-72808260 None:intergenic 30.0%
TGGTGAACATTACTAAAATG+AGG - chr2_1:72808248-72808267 None:intergenic 30.0%
! TACCAATTTTGTAATGAGGT+GGG - chr2_1:72808374-72808393 None:intergenic 30.0%
! CTTGTACCAATTTTGTAATG+AGG - chr2_1:72808378-72808397 None:intergenic 30.0%
CCAATTTCAACAATGATTTC+CGG - chr2_1:72808582-72808601 None:intergenic 30.0%
!!! TACAATGGTTTTTTAACCGA+CGG + chr2_1:72808651-72808670 Msa0223270:intron 30.0%
ACGGTTAATAATAAATTGCG+AGG + chr2_1:72808791-72808810 Msa0223270:intron 30.0%
TGGATAGCATAATTTAGTCT+TGG - chr2_1:72808921-72808940 None:intergenic 30.0%
TTCTTAAATGACAGTAAAGG+TGG - chr2_1:72808941-72808960 None:intergenic 30.0%
GAGTATAGTACCAATTATTC+AGG + chr2_1:72809054-72809073 Msa0223270:intron 30.0%
TCAGGAAAATGATATACAGT+TGG + chr2_1:72809072-72809091 Msa0223270:intron 30.0%
ACAAAAGAGTCGAATTACAT+GGG - chr2_1:72809309-72809328 None:intergenic 30.0%
AACAAAAGAGTCGAATTACA+TGG - chr2_1:72809310-72809329 None:intergenic 30.0%
CCAAATTGACAGTGTTTAAA+TGG - chr2_1:72809476-72809495 None:intergenic 30.0%
CCATTTAAACACTGTCAATT+TGG + chr2_1:72809473-72809492 Msa0223270:intron 30.0%
CACTTGATTATAGCAAGAAA+AGG - chr2_1:72809719-72809738 None:intergenic 30.0%
GATGCAAAACTTTACTGATA+AGG - chr2_1:72809752-72809771 None:intergenic 30.0%
! CAAAATATTGGTAGCATACT+TGG - chr2_1:72809857-72809876 None:intergenic 30.0%
!! TGTGTATTTTTGTTGTCAAC+AGG + chr2_1:72809923-72809942 Msa0223270:intron 30.0%
CTTATTAGGAAGTTCAGATA+AGG - chr2_1:72810101-72810120 None:intergenic 30.0%
GAAATGATCATCATCTACAA+AGG - chr2_1:72810148-72810167 None:intergenic 30.0%
GGATTAATATGGCATATTCT+TGG - chr2_1:72810191-72810210 None:intergenic 30.0%
TGCCTCTTATGCATATTTAT+TGG + chr2_1:72810237-72810256 Msa0223270:CDS 30.0%
GCCTCTTATGCATATTTATT+GGG + chr2_1:72810238-72810257 Msa0223270:CDS 30.0%
ACCCAATAAATATGCATAAG+AGG - chr2_1:72810242-72810261 None:intergenic 30.0%
AATGAGAAAATGAGAGGAAA+CGG - chr2_1:72810495-72810514 None:intergenic 30.0%
AACAAGAATGAGAAAATGAG+AGG - chr2_1:72810501-72810520 None:intergenic 30.0%
TTTGCTTATTGAATTCTCCT+TGG + chr2_1:72810676-72810695 Msa0223270:three_prime_UTR 30.0%
ATACTACGTTCCAAGTAAAA+CGG - chr2_1:72810748-72810767 None:intergenic 30.0%
! GACACTCTTTTCAAAACATA+TGG - chr2_1:72810775-72810794 None:intergenic 30.0%
TCTGAATCCTGATCAATCAA+GGG - chr2_1:72807435-72807454 None:intergenic 35.0%
TTCTGAATCCTGATCAATCA+AGG - chr2_1:72807436-72807455 None:intergenic 35.0%
CGTATAAAGCAAAAGTTCAC+CGG + chr2_1:72807502-72807521 Msa0223270:five_prime_UTR 35.0%
TGAATTTGGGTAAAAGGGTT+TGG - chr2_1:72807675-72807694 None:intergenic 35.0%
GAAAGGTGAATTTGGGTAAA+AGG - chr2_1:72807681-72807700 None:intergenic 35.0%
AAAACGCAACAGTTTCAAGA+TGG - chr2_1:72808107-72808126 None:intergenic 35.0%
TAATCAAATTGGTCCATCCT+TGG + chr2_1:72808213-72808232 Msa0223270:intron 35.0%
TTCCCACCTCATTACAAAAT+TGG + chr2_1:72808369-72808388 Msa0223270:intron 35.0%
! GTACCAATTTTGTAATGAGG+TGG - chr2_1:72808375-72808394 None:intergenic 35.0%
! TTCAGCATGTAAGGCTTTTA+TGG - chr2_1:72808443-72808462 None:intergenic 35.0%
CATGAAACTGAACCCAATTA+AGG - chr2_1:72808495-72808514 None:intergenic 35.0%
TAGTATATGGGGAAAACAAG+TGG + chr2_1:72808535-72808554 Msa0223270:intron 35.0%
CCGGAAATCATTGTTGAAAT+TGG + chr2_1:72808579-72808598 Msa0223270:intron 35.0%
ATCAATATATTGGTCATGGC+AGG + chr2_1:72808628-72808647 Msa0223270:intron 35.0%
! TGTACTTAAACCGTTGACTA+CGG + chr2_1:72808772-72808791 Msa0223270:intron 35.0%
GTGAATACTATTGATCGTGA+GGG - chr2_1:72808881-72808900 None:intergenic 35.0%
TGTGAATACTATTGATCGTG+AGG - chr2_1:72808882-72808901 None:intergenic 35.0%
!!! ACTGACTTCATGGTTTTAGT+AGG + chr2_1:72809097-72809116 Msa0223270:intron 35.0%
GAACCTTGCAATTATGCTTA+AGG + chr2_1:72809273-72809292 Msa0223270:intron 35.0%
GTACCAAAACTGGTTAAGTT+TGG + chr2_1:72809396-72809415 Msa0223270:intron 35.0%
! GAACCAAACTTAACCAGTTT+TGG - chr2_1:72809402-72809421 None:intergenic 35.0%
TTCAAAATCTCACGAGTTGT+TGG - chr2_1:72809794-72809813 None:intergenic 35.0%
CAACAGGAAGCAAAAACAAT+AGG + chr2_1:72809939-72809958 Msa0223270:intron 35.0%
AGCAGAAACAGGGATTAATA+TGG - chr2_1:72810202-72810221 None:intergenic 35.0%
!! ATATTAATCCCTGTTTCTGC+TGG + chr2_1:72810201-72810220 Msa0223270:CDS 35.0%
ACATTATGCCTTCTTCTTCT+TGG - chr2_1:72810280-72810299 None:intergenic 35.0%
! CACTTTAAAAGGTGATACCA+AGG - chr2_1:72810696-72810715 None:intergenic 35.0%
! TTTTCATTCCCCGTTTTACT+TGG + chr2_1:72810735-72810754 Msa0223270:three_prime_UTR 35.0%
TACTACGTTCCAAGTAAAAC+GGG - chr2_1:72810747-72810766 None:intergenic 35.0%
CTTTATACGATCGGTTGAGA+AGG - chr2_1:72807491-72807510 None:intergenic 40.0%
! ATGTTACTTACCGTTCACGA+CGG - chr2_1:72807554-72807573 None:intergenic 40.0%
! GATGTTGGGTTGTTATAAGC+TGG + chr2_1:72807743-72807762 Msa0223270:intron 40.0%
!! ATGGGCTGACATTGTTGTTT+AGG + chr2_1:72807947-72807966 Msa0223270:intron 40.0%
AGAAACATCATGAGCAAGGT+CGG - chr2_1:72808084-72808103 None:intergenic 40.0%
ATGGAGAAACATCATGAGCA+AGG - chr2_1:72808088-72808107 None:intergenic 40.0%
AAATGAGGATTGAGGAACCA+AGG - chr2_1:72808233-72808252 None:intergenic 40.0%
TGAACTCATAGTGAAGCAAG+TGG - chr2_1:72808268-72808287 None:intergenic 40.0%
ATGCTGAAGCTTCTACCAAT+GGG + chr2_1:72808455-72808474 Msa0223270:intron 40.0%
GAGAAAAGAATCATCCCCAT+TGG - chr2_1:72808473-72808492 None:intergenic 40.0%
!! GGGGAAAACAAGTGGTTTTA+AGG + chr2_1:72808543-72808562 Msa0223270:intron 40.0%
! GCTGTTGCGCATTTAAAGTT+TGG + chr2_1:72809178-72809197 Msa0223270:intron 40.0%
CTGCCTTAAGCATAATTGCA+AGG - chr2_1:72809279-72809298 None:intergenic 40.0%
! TAAGTTTGGTTCGACTGAAC+TGG + chr2_1:72809410-72809429 Msa0223270:intron 40.0%
! GAACTGGTTTCTTGTTAGTC+CGG + chr2_1:72809426-72809445 Msa0223270:intron 40.0%
!!! TACGTTTTGGGTTATCATCC+TGG + chr2_1:72810037-72810056 Msa0223270:CDS 40.0%
TACTTGGCATGTTACTAACC+AGG - chr2_1:72810058-72810077 None:intergenic 40.0%
GCTGAAATCCAAGAAGAAGA+AGG + chr2_1:72810269-72810288 Msa0223270:CDS 40.0%
ATGACCCTAGTGTCATGAAT+TGG + chr2_1:72810379-72810398 Msa0223270:three_prime_UTR 40.0%
GTAGCCAATTCATGACACTA+GGG - chr2_1:72810386-72810405 None:intergenic 40.0%
GCTACAAAAACGAGGTAATG+TGG - chr2_1:72810408-72810427 None:intergenic 40.0%
!! CATTACCTCGTTTTTGTAGC+TGG + chr2_1:72810408-72810427 Msa0223270:three_prime_UTR 40.0%
GTGCTGTAGATGCTACATTA+AGG - chr2_1:72810450-72810469 None:intergenic 40.0%
CAAAATTGTTTGTGTCGCCT+TGG + chr2_1:72810593-72810612 Msa0223270:three_prime_UTR 40.0%
ACTACGTTCCAAGTAAAACG+GGG - chr2_1:72810746-72810765 None:intergenic 40.0%
TGGGTTGTTATAAGCTGGAC+TGG + chr2_1:72807748-72807767 Msa0223270:intron 45.0%
! TTATAAGCTGGACTGGAGTG+AGG + chr2_1:72807755-72807774 Msa0223270:intron 45.0%
! TATAAGCTGGACTGGAGTGA+GGG + chr2_1:72807756-72807775 Msa0223270:intron 45.0%
GAAACATCATGAGCAAGGTC+GGG - chr2_1:72808083-72808102 None:intergenic 45.0%
GGTAGAAGCTTCAGCATGTA+AGG - chr2_1:72808452-72808471 None:intergenic 45.0%
CATGCTGAAGCTTCTACCAA+TGG + chr2_1:72808454-72808473 Msa0223270:intron 45.0%
TGCTGAAGCTTCTACCAATG+GGG + chr2_1:72808456-72808475 Msa0223270:intron 45.0%
ATTGGTCATGGCAGGTACAA+TGG + chr2_1:72808636-72808655 Msa0223270:intron 45.0%
GAAACTGGGCATTTAACCGT+CGG - chr2_1:72808670-72808689 None:intergenic 45.0%
ATATCTAACGCTTTACCGGC+AGG + chr2_1:72808839-72808858 Msa0223270:intron 45.0%
!! ACAGTTGGTGACTGACTTCA+TGG + chr2_1:72809087-72809106 Msa0223270:intron 45.0%
GGAAACTCACTGAGACAAGT+AGG + chr2_1:72809118-72809137 Msa0223270:intron 45.0%
GGGCGTTATAGTACCAAAAC+TGG + chr2_1:72809386-72809405 Msa0223270:intron 45.0%
AAAGTCTGCAGTGAGCTGAA+AGG - chr2_1:72809819-72809838 None:intergenic 45.0%
GAATTAGCAGACAGAGCAGT+TGG + chr2_1:72809972-72809991 Msa0223270:CDS 45.0%
GGTAGCCAATTCATGACACT+AGG - chr2_1:72810387-72810406 None:intergenic 45.0%
!! TACCTCGTTTTTGTAGCTGG+CGG + chr2_1:72810411-72810430 Msa0223270:three_prime_UTR 45.0%
TAGCATCTACAGCACACAAC+AGG + chr2_1:72810455-72810474 Msa0223270:three_prime_UTR 45.0%
AGCATCTACAGCACACAACA+GGG + chr2_1:72810456-72810475 Msa0223270:three_prime_UTR 45.0%
TACAGCACACAACAGGGATT+TGG + chr2_1:72810462-72810481 Msa0223270:three_prime_UTR 45.0%
GAAAATGAGAGGAAACGGAG+AGG - chr2_1:72810490-72810509 None:intergenic 45.0%
CTCCTTACATGAGGGTGAAA+AGG + chr2_1:72810616-72810635 Msa0223270:three_prime_UTR 45.0%
! CTCCTTTTCACCCTCATGTA+AGG - chr2_1:72810621-72810640 None:intergenic 45.0%
TACATGAGGGTGAAAAGGAG+AGG + chr2_1:72810621-72810640 Msa0223270:three_prime_UTR 45.0%
TCATCGTCTTCCGTCGTGAA+CGG + chr2_1:72807541-72807560 Msa0223270:five_prime_UTR 50.0%
GAGGATTGAGGAACCAAGGA+TGG - chr2_1:72808229-72808248 None:intergenic 50.0%
ACTCAACAGTATCCACCTGC+CGG - chr2_1:72808857-72808876 None:intergenic 50.0%
GAGCAACACCAGCAGAAACA+GGG - chr2_1:72810212-72810231 None:intergenic 50.0%
AGAGCAACACCAGCAGAAAC+AGG - chr2_1:72810213-72810232 None:intergenic 50.0%
!! ACCTCGTTTTTGTAGCTGGC+GGG + chr2_1:72810412-72810431 Msa0223270:three_prime_UTR 50.0%
AGTTCACCGGAAAAAGCCGC+CGG + chr2_1:72807515-72807534 Msa0223270:five_prime_UTR 55.0%
!! TGAACTCCGGCGGCTTTTTC+CGG - chr2_1:72807524-72807543 None:intergenic 55.0%
CGACGGAAGACGATGAACTC+CGG - chr2_1:72807537-72807556 None:intergenic 55.0%
TCTAACGCTTTACCGGCAGG+TGG + chr2_1:72808842-72808861 Msa0223270:intron 55.0%
!! CCTCGTTTTTGTAGCTGGCG+GGG + chr2_1:72810413-72810432 Msa0223270:three_prime_UTR 55.0%
TCGCCTTGGCTCCTTACATG+AGG + chr2_1:72810607-72810626 Msa0223270:three_prime_UTR 55.0%
CGCCTTGGCTCCTTACATGA+GGG + chr2_1:72810608-72810627 Msa0223270:three_prime_UTR 55.0%
CACCCTCATGTAAGGAGCCA+AGG - chr2_1:72810613-72810632 None:intergenic 55.0%
CGGAAGACGATGAACTCCGG+CGG - chr2_1:72807534-72807553 None:intergenic 60.0%
CCCCGCCAGCTACAAAAACG+AGG - chr2_1:72810416-72810435 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 72807389 72810824 72807389 ID=Msa0223270;Name=Msa0223270
chr2_1 mRNA 72807389 72810824 72807389 ID=Msa0223270-mRNA-1;Parent=Msa0223270;Name=Msa0223270-mRNA-1;_AED=0.02;_eAED=0.02;_QI=298|1|1|1|0.33|0.5|4|528|80
chr2_1 exon 72807389 72807562 72807389 ID=Msa0223270-mRNA-1:exon:21121;Parent=Msa0223270-mRNA-1
chr2_1 exon 72809758 72809857 72809758 ID=Msa0223270-mRNA-1:exon:21122;Parent=Msa0223270-mRNA-1
chr2_1 exon 72809945 72810058 72809945 ID=Msa0223270-mRNA-1:exon:21123;Parent=Msa0223270-mRNA-1
chr2_1 exon 72810144 72810824 72810144 ID=Msa0223270-mRNA-1:exon:21124;Parent=Msa0223270-mRNA-1
chr2_1 five_prime_UTR 72807389 72807562 72807389 ID=Msa0223270-mRNA-1:five_prime_utr;Parent=Msa0223270-mRNA-1
chr2_1 five_prime_UTR 72809758 72809857 72809758 ID=Msa0223270-mRNA-1:five_prime_utr;Parent=Msa0223270-mRNA-1
chr2_1 five_prime_UTR 72809945 72809968 72809945 ID=Msa0223270-mRNA-1:five_prime_utr;Parent=Msa0223270-mRNA-1
chr2_1 CDS 72809969 72810058 72809969 ID=Msa0223270-mRNA-1:cds;Parent=Msa0223270-mRNA-1
chr2_1 CDS 72810144 72810296 72810144 ID=Msa0223270-mRNA-1:cds;Parent=Msa0223270-mRNA-1
chr2_1 three_prime_UTR 72810297 72810824 72810297 ID=Msa0223270-mRNA-1:three_prime_utr;Parent=Msa0223270-mRNA-1
Gene Sequence

>Msa0223270

ATGGAATTAGCAGACAGAGCAGTTGGATTTCTATTGTCCTTTATAAGCTTATCGATATTCACTTATTATACGTTTTGGGTTATCATCCTGCCCTTTGTAGATGATGATCATTTCGTACACAAGTATTTCTTGCCCCAAGAATATGCCATATTAATCCCTGTTTCTGCTGGTGTTGCTCTTATTTGCCTCTTATGCATATTTATTGGGTTCGTGATGCTGAAATCCAAGAAGAAGAAGGCATAA
Protein sequence

>Msa0223270

MELADRAVGFLLSFISLSIFTYYTFWVIILPFVDDDHFVHKYFLPQEYAILIPVSAGVALICLLCIFIGFVMLKSKKKKA*