AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225780 MtrunA17_Chr2g0332141 91.667 120 10 0 102 221 4 123 2.58e-80 236
Msa0225780 MtrunA17_Chr1g0190711 42.793 222 83 7 24 202 17 237 1.85e-48 159
Msa0225780 MtrunA17_Chr6g0486121 39.224 232 91 9 17 202 11 238 2.94e-46 154
Msa0225780 MtrunA17_Chr1g0184791 42.793 222 79 11 23 202 14 229 1.38e-44 149
Msa0225780 MtrunA17_Chr1g0187921 43.056 216 81 8 24 202 10 220 2.54e-44 148
Msa0225780 MtrunA17_Chr7g0260641 41.850 227 79 9 24 202 13 234 1.08e-43 147
Msa0225780 MtrunA17_Chr8g0390281 45.122 164 63 4 63 202 117 277 5.02e-41 141
Msa0225780 MtrunA17_Chr1g0187961 43.617 188 71 7 24 202 13 174 1.39e-40 137
Msa0225780 MtrunA17_Chr1g0166011 40.741 189 83 6 27 202 6 178 1.04e-39 134
Msa0225780 MtrunA17_Chr5g0428141 43.787 169 69 6 73 219 183 347 7.25e-39 137
Msa0225780 MtrunA17_Chr1g0184801 39.796 196 89 5 24 202 14 197 9.19e-39 133
Msa0225780 MtrunA17_Chr7g0260621 41.089 202 77 8 25 202 14 197 3.21e-38 131
Msa0225780 MtrunA17_Chr5g0411501 39.655 174 76 5 68 216 163 332 2.30e-37 132
Msa0225780 MtrunA17_Chr2g0331411 39.691 194 78 6 51 219 147 326 4.09e-37 132
Msa0225780 MtrunA17_Chr8g0366881 41.139 158 65 5 83 216 192 345 4.05e-35 127
Msa0225780 MtrunA17_Chr4g0004181 38.660 194 88 6 24 214 8 173 1.05e-30 111
Msa0225780 MtrunA17_Chr2g0330951 32.515 163 68 2 82 202 92 254 7.23e-22 90.5
Msa0225780 MtrunA17_Chr8g0340731 34.078 179 79 5 61 202 84 260 9.29e-22 90.5
Msa0225780 MtrunA17_Chr1g0183251 41.667 108 54 2 106 204 147 254 1.39e-20 87.4
Msa0225780 MtrunA17_Chr4g0069561 33.083 133 72 4 70 197 58 178 1.53e-19 82.4
Msa0225780 MtrunA17_Chr1g0193861 30.328 122 69 3 81 197 58 168 3.19e-14 68.2
Msa0225780 MtrunA17_Chr7g0270851 33.663 101 59 2 97 197 61 153 5.31e-14 67.4
Msa0225780 MtrunA17_Chr4g0064131 31.915 141 62 4 77 200 83 206 9.37e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225780 AT1G04250.1 45.116 215 87 8 24 210 9 220 3.44e-52 168
Msa0225780 AT4G14550.1 44.651 215 78 8 24 202 3 212 2.78e-50 163
Msa0225780 AT4G14550.2 44.651 215 78 8 24 202 3 212 4.48e-50 163
Msa0225780 AT3G04730.1 42.986 221 80 7 24 202 4 220 2.69e-47 156
Msa0225780 AT3G23050.1 43.498 223 78 9 24 202 8 226 1.58e-45 152
Msa0225780 AT4G14550.3 42.424 198 73 8 24 185 3 195 2.98e-40 137
Msa0225780 AT1G04240.1 40.212 189 89 5 24 202 7 181 7.01e-39 133
Msa0225780 AT3G23030.1 41.304 184 84 7 19 202 7 166 1.80e-38 131
Msa0225780 AT4G29080.1 35.521 259 89 8 18 202 35 289 4.01e-38 134
Msa0225780 AT3G23030.2 41.304 184 84 7 19 202 94 253 1.04e-37 132
Msa0225780 AT5G43700.1 38.503 187 85 6 24 202 13 177 1.31e-37 129
Msa0225780 AT1G04240.2 40.212 189 89 5 24 202 107 281 1.48e-37 132
Msa0225780 AT3G23050.2 39.623 212 70 9 24 186 8 210 1.21e-35 125
Msa0225780 AT3G15540.1 39.109 202 86 9 24 208 8 189 6.55e-35 123
Msa0225780 AT5G65670.1 44.604 139 54 5 82 198 179 316 7.19e-35 127
Msa0225780 AT5G65670.2 42.038 157 67 6 82 216 179 333 7.68e-35 126
Msa0225780 AT2G22670.5 36.946 203 91 7 32 209 119 309 1.77e-34 125
Msa0225780 AT2G22670.2 36.946 203 91 7 32 209 119 309 1.77e-34 125
Msa0225780 AT1G15580.1 40.110 182 67 6 24 198 10 156 2.22e-34 120
Msa0225780 AT1G52830.1 37.368 190 102 5 24 208 8 185 2.76e-34 121
Msa0225780 AT2G22670.3 36.735 196 88 6 32 202 119 303 1.11e-33 123
Msa0225780 AT2G22670.1 36.735 196 88 6 32 202 119 303 1.11e-33 123
Msa0225780 AT2G22670.4 36.735 196 88 6 32 202 136 320 1.49e-33 123
Msa0225780 AT4G14560.1 39.583 192 84 9 14 202 1 163 3.94e-33 117
Msa0225780 AT3G16500.1 33.188 229 104 7 19 202 28 252 5.80e-27 104
Msa0225780 AT3G16500.2 33.188 229 104 7 19 202 28 252 7.80e-27 103
Msa0225780 AT1G51950.1 31.481 216 105 4 25 202 40 250 3.11e-24 97.4
Msa0225780 AT4G14550.4 40.268 149 54 5 24 141 3 147 5.34e-23 92.0
Msa0225780 AT2G33310.2 37.838 148 81 5 65 204 85 229 2.25e-22 92.0
Msa0225780 AT2G33310.3 37.838 148 81 5 65 204 84 228 2.35e-22 91.7
Msa0225780 AT2G33310.1 37.838 148 81 5 65 204 84 228 2.35e-22 91.7
Msa0225780 AT1G80390.1 45.378 119 43 5 27 139 19 121 7.50e-22 87.4
Msa0225780 AT3G17600.1 32.000 150 73 4 62 197 18 152 2.10e-21 87.0
Msa0225780 AT1G04550.2 29.897 194 80 7 50 202 41 219 7.80e-21 87.8
Msa0225780 AT5G25890.1 34.884 129 55 2 87 201 49 162 1.92e-20 85.1
Msa0225780 AT4G28640.3 34.667 150 65 3 86 202 88 237 2.73e-20 86.3
Msa0225780 AT4G28640.1 34.667 150 65 3 86 202 88 237 3.48e-20 85.9
Msa0225780 AT3G23050.3 36.875 160 53 6 24 141 8 161 2.15e-19 82.0
Msa0225780 AT4G28640.2 34.667 150 65 3 86 202 88 237 2.28e-19 84.7
Msa0225780 AT1G04100.1 40.952 105 48 3 107 201 154 254 1.05e-17 79.7
Msa0225780 AT3G62100.1 40.217 92 46 2 106 197 84 166 6.08e-16 73.2
Msa0225780 AT2G46990.1 37.634 93 47 2 106 197 86 168 2.93e-15 71.2
Msa0225780 AT4G32280.2 36.559 93 45 1 106 198 119 197 3.20e-14 69.3
Msa0225780 AT4G32280.1 36.559 93 45 1 106 198 161 239 8.69e-14 68.9
Msa0225780 AT3G62100.2 40.741 81 39 2 106 186 84 155 1.46e-11 60.8
Msa0225780 AT2G46990.2 39.024 82 39 2 106 186 86 157 2.48e-11 60.5

Find 75 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGAGGACAATATTGATTT+TGG 0.202496 2_1:+75920660 Msa0225780:CDS
CGTTCAAAGTTGTGTATATT+AGG 0.211169 2_1:-75919529 None:intergenic
GCTATAAAATGGACCGGGTT+TGG 0.277811 2_1:+75920976 Msa0225780:three_prime_UTR
AAAAGACTCTAGCTATAAAA+TGG 0.286074 2_1:+75920965 Msa0225780:three_prime_UTR
AACCAGGGGTGGTCTTTATT+AGG 0.300336 2_1:+75921009 Msa0225780:three_prime_UTR
TGAGCAAGTAATTGTCCATT+AGG 0.316383 2_1:-75921151 None:intergenic
GATGAATGAGAAGAAATTCA+TGG 0.319723 2_1:+75920310 Msa0225780:CDS
CTATAAAATGGACCGGGTTT+GGG 0.321469 2_1:+75920977 Msa0225780:three_prime_UTR
TTGAATCATGTAAGAGGATA+AGG 0.340536 2_1:+75920625 Msa0225780:CDS
CTTATTATTCGTACATTCAA+TGG 0.347580 2_1:-75919635 None:intergenic
TAATAAGATGAATATTATTC+AGG 0.355657 2_1:+75919651 Msa0225780:CDS
GGTTCTTACTTTGATGTTGT+TGG 0.366642 2_1:-75919835 None:intergenic
GTTCTTACTTTGATGTTGTT+GGG 0.367350 2_1:-75919834 None:intergenic
CTCACTGGAGGCCGTACATC+TGG 0.369783 2_1:-75920797 None:intergenic
GAGCTTACCCTTGGTCTTCC+CGG 0.380531 2_1:+75919679 Msa0225780:CDS
TACGTGAAAGTTGCGGTTGA+TGG 0.380805 2_1:+75920027 Msa0225780:CDS
GTTTGGGTGCAGTTGGAACC+AGG 0.383484 2_1:+75920993 Msa0225780:three_prime_UTR
TGAGAATGATTAGTTTCTAT+TGG 0.397999 2_1:-75921192 None:intergenic
TTGGGTGCAGTTGGAACCAG+GGG 0.404325 2_1:+75920995 Msa0225780:three_prime_UTR
GAGCAAGTAATTGTCCATTA+GGG 0.435225 2_1:-75921150 None:intergenic
CTTTGAGAAATGATTACATC+AGG 0.440422 2_1:+75921067 Msa0225780:three_prime_UTR
ATGGTGACTGGATGTTGCTT+GGG 0.460851 2_1:+75920381 Msa0225780:CDS
ACATGTGGTGGGATGGCCAC+CGG 0.463192 2_1:+75919966 Msa0225780:CDS
TATATAACTCAATGTGGTTA+CGG 0.469542 2_1:-75919571 None:intergenic
GGTGGGATGGCCACCGGTGA+GGG 0.473868 2_1:+75919972 Msa0225780:CDS
AATAGTATATAAAACCCTAA+TGG 0.484093 2_1:+75921136 Msa0225780:three_prime_UTR
TTTATCAACCTCCAACTTGT+GGG 0.484171 2_1:-75921232 None:intergenic
ACTCTAGCTATAAAATGGAC+CGG 0.484208 2_1:+75920970 Msa0225780:three_prime_UTR
TGTTTGTTGAATCATGTAAG+AGG 0.487798 2_1:+75920619 Msa0225780:CDS
TGATGTTGTTGGGAATTTAG+TGG 0.488099 2_1:-75919824 None:intergenic
GGTGCAGTTGGAACCAGGGG+TGG 0.495977 2_1:+75920998 Msa0225780:three_prime_UTR
CAAGATAAAATGAACAATAG+AGG 0.499260 2_1:+75921035 Msa0225780:three_prime_UTR
GCTATAATTATTCTTCTCAC+TGG 0.500501 2_1:-75920812 None:intergenic
TGGAGCTTGTACACTTTGAA+AGG 0.503826 2_1:-75920897 None:intergenic
TGGTGGGATGGCCACCGGTG+AGG 0.504156 2_1:+75919971 Msa0225780:CDS
ATGAGAAGTACTAGAAGTTG+AGG 0.504166 2_1:-75919773 None:intergenic
GATGGTGACTGGATGTTGCT+TGG 0.516052 2_1:+75920380 Msa0225780:CDS
CAGGAGACTGAGCTTACCCT+TGG 0.519336 2_1:+75919670 Msa0225780:CDS
TATATATGAACTTACTTCCA+TGG 0.525569 2_1:-75920410 None:intergenic
TTTGGGTGCAGTTGGAACCA+GGG 0.526314 2_1:+75920994 Msa0225780:three_prime_UTR
GGACCGGGTTTGGGTGCAGT+TGG 0.527922 2_1:+75920986 Msa0225780:three_prime_UTR
TTCCTAATAAAGACCACCCC+TGG 0.529842 2_1:-75921011 None:intergenic
AGCTCCATATCTAAGAAAAG+TGG 0.534799 2_1:+75920050 Msa0225780:CDS
TGCATGCAGGGAACATGTGG+TGG 0.534930 2_1:+75919954 Msa0225780:intron
GTTCCAACTGCACCCAAACC+CGG 0.536898 2_1:-75920989 None:intergenic
GCAGGGAACATGTGGTGGGA+TGG 0.542566 2_1:+75919959 Msa0225780:intron
TGTTGCAGGTTCCAGATGTA+CGG 0.542574 2_1:+75920786 Msa0225780:intron
TTCCTACTTGCCCTCACCGG+TGG 0.558621 2_1:-75919982 None:intergenic
CTCTAGCTATAAAATGGACC+GGG 0.559995 2_1:+75920971 Msa0225780:three_prime_UTR
ATAATTATTCTTCTCACTGG+AGG 0.565078 2_1:-75920809 None:intergenic
ACGAAGATAAAGATGGTGAC+TGG 0.565436 2_1:+75920369 Msa0225780:CDS
GCATGCAGGGAACATGTGGT+GGG 0.566681 2_1:+75919955 Msa0225780:intron
TATCAACCTCCAACTTGTGG+GGG 0.569967 2_1:-75921230 None:intergenic
GGTAGTAGTACCAAAACTGC+AGG 0.586869 2_1:+75919700 Msa0225780:CDS
TTATCAACCTCCAACTTGTG+GGG 0.587139 2_1:-75921231 None:intergenic
GATAAGGCTTATGATTAGCT+CGG 0.592824 2_1:+75920641 Msa0225780:CDS
GCTACATACGAAGATAAAGA+TGG 0.600922 2_1:+75920362 Msa0225780:CDS
CAGGTATATATACATAAGAG+AGG 0.601747 2_1:+75921086 Msa0225780:three_prime_UTR
TGGCAATTAGATGAAGATCA+AGG 0.605901 2_1:-75919750 None:intergenic
ATCAACCTCCAACTTGTGGG+GGG 0.613816 2_1:-75921229 None:intergenic
GGGTGCATGCAGGGAACATG+TGG 0.617054 2_1:+75919951 Msa0225780:intron
TACTACTACCGGGAAGACCA+AGG 0.619025 2_1:-75919687 None:intergenic
TTGCAAGTACGTGAAAGTTG+CGG 0.621140 2_1:+75920020 Msa0225780:CDS
GGCCACCGGTGAGGGCAAGT+AGG 0.630880 2_1:+75919980 Msa0225780:CDS
GAAGCAATTAGTAGCAAACA+TGG 0.636180 2_1:-75920121 None:intergenic
AGAAGTACTAGAAGTTGAGG+TGG 0.639519 2_1:-75919770 None:intergenic
TGTTGCTTGGGGATGCACCA+TGG 0.646753 2_1:+75920393 Msa0225780:CDS
AGGTATATATACATAAGAGA+GGG 0.648408 2_1:+75921087 Msa0225780:three_prime_UTR
ATATAGCGATGATCATAATG+TGG 0.656981 2_1:-75920942 None:intergenic
TCGTACATTCAATGGAACTG+TGG 0.659415 2_1:-75919627 None:intergenic
ACTACTACCGGGAAGACCAA+GGG 0.663756 2_1:-75919686 None:intergenic
ATTGTATATATAACTCAATG+TGG 0.669958 2_1:-75919577 None:intergenic
TGGTGACTGGATGTTGCTTG+GGG 0.674509 2_1:+75920382 Msa0225780:CDS
ACAATGTCATCACAAATGAG+TGG 0.687436 2_1:+75919595 Msa0225780:exon
AAGGCTTATGATTAGCTCGG+AGG 0.705152 2_1:+75920644 Msa0225780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATTAATGTAAAGT+TGG + chr2_1:75919930-75919949 Msa0225780:intron 10.0%
!! AAATTAATTAATGTAAAGTT+GGG + chr2_1:75919931-75919950 Msa0225780:intron 10.0%
!! TTTATACACAATATAATTAT+AGG + chr2_1:75920714-75920733 Msa0225780:intron 10.0%
!! AAAATTAATTAATGTAAAGT+TGG + chr2_1:75919930-75919949 Msa0225780:intron 10.0%
!! AAATTAATTAATGTAAAGTT+GGG + chr2_1:75919931-75919950 Msa0225780:intron 10.0%
!! TTTATACACAATATAATTAT+AGG + chr2_1:75920714-75920733 Msa0225780:intron 10.0%
!! TAATAAGATGAATATTATTC+AGG + chr2_1:75919651-75919670 Msa0225780:CDS 15.0%
!! TATGAAACATAAAAATAACA+TGG - chr2_1:75920201-75920220 None:intergenic 15.0%
!!! TAATATTTTATATTCTAGCT+AGG + chr2_1:75920871-75920890 Msa0225780:three_prime_UTR 15.0%
!! TAATAAGATGAATATTATTC+AGG + chr2_1:75919651-75919670 Msa0225780:CDS 15.0%
!! TATGAAACATAAAAATAACA+TGG - chr2_1:75920201-75920220 None:intergenic 15.0%
!!! TAATATTTTATATTCTAGCT+AGG + chr2_1:75920871-75920890 Msa0225780:three_prime_UTR 15.0%
!! ATTGTATATATAACTCAATG+TGG - chr2_1:75919580-75919599 None:intergenic 20.0%
!! AAAAAATAGGAATGAATACA+TGG + chr2_1:75920340-75920359 Msa0225780:CDS 20.0%
!! TTTGATGAATATATACTCTA+AGG - chr2_1:75920513-75920532 None:intergenic 20.0%
!! ATTGATTGATATCATAAGTT+TGG + chr2_1:75920737-75920756 Msa0225780:intron 20.0%
!!! TAGTACATGCTTTTTTTAAT+TGG - chr2_1:75920920-75920939 None:intergenic 20.0%
!! AATAGTATATAAAACCCTAA+TGG + chr2_1:75921136-75921155 Msa0225780:three_prime_UTR 20.0%
!! ATTGTATATATAACTCAATG+TGG - chr2_1:75919580-75919599 None:intergenic 20.0%
!! AAAAAATAGGAATGAATACA+TGG + chr2_1:75920340-75920359 Msa0225780:CDS 20.0%
!! TTTGATGAATATATACTCTA+AGG - chr2_1:75920513-75920532 None:intergenic 20.0%
!! ATTGATTGATATCATAAGTT+TGG + chr2_1:75920737-75920756 Msa0225780:intron 20.0%
!!! TAGTACATGCTTTTTTTAAT+TGG - chr2_1:75920920-75920939 None:intergenic 20.0%
!! AATAGTATATAAAACCCTAA+TGG + chr2_1:75921136-75921155 Msa0225780:three_prime_UTR 20.0%
! TATATAACTCAATGTGGTTA+CGG - chr2_1:75919574-75919593 None:intergenic 25.0%
! CTTATTATTCGTACATTCAA+TGG - chr2_1:75919638-75919657 None:intergenic 25.0%
! TACATATATGTTGTGTGTTA+TGG + chr2_1:75920230-75920249 Msa0225780:intron 25.0%
! ACATATATGTTGTGTGTTAT+GGG + chr2_1:75920231-75920250 Msa0225780:intron 25.0%
! CTGCAATAATTAATGATAGT+TGG - chr2_1:75920280-75920299 None:intergenic 25.0%
! CAACTATCATTAATTATTGC+AGG + chr2_1:75920278-75920297 Msa0225780:intron 25.0%
!! TCATGGATTCTAGAAAAAAT+AGG + chr2_1:75920327-75920346 Msa0225780:CDS 25.0%
! TATATATGAACTTACTTCCA+TGG - chr2_1:75920413-75920432 None:intergenic 25.0%
! AAATAATAACTAGCTGTAGA+TGG - chr2_1:75920446-75920465 None:intergenic 25.0%
!!! TTTTTTTCTTTCTTTCCTCT+AGG + chr2_1:75920595-75920614 Msa0225780:intron 25.0%
! AACAATATTAGTAAATCAGG+GGG - chr2_1:75920770-75920789 None:intergenic 25.0%
! CAACAATATTAGTAAATCAG+GGG - chr2_1:75920771-75920790 None:intergenic 25.0%
! GCAACAATATTAGTAAATCA+GGG - chr2_1:75920772-75920791 None:intergenic 25.0%
! TGCAACAATATTAGTAAATC+AGG - chr2_1:75920773-75920792 None:intergenic 25.0%
! TGATTTACTAATATTGTTGC+AGG + chr2_1:75920772-75920791 Msa0225780:intron 25.0%
! AAAAGACTCTAGCTATAAAA+TGG + chr2_1:75920965-75920984 Msa0225780:three_prime_UTR 25.0%
! CAAGATAAAATGAACAATAG+AGG + chr2_1:75921035-75921054 Msa0225780:three_prime_UTR 25.0%
! AGGTATATATACATAAGAGA+GGG + chr2_1:75921087-75921106 Msa0225780:three_prime_UTR 25.0%
!!! TGAGAATGATTAGTTTCTAT+TGG - chr2_1:75921195-75921214 None:intergenic 25.0%
! TATATAACTCAATGTGGTTA+CGG - chr2_1:75919574-75919593 None:intergenic 25.0%
! CTTATTATTCGTACATTCAA+TGG - chr2_1:75919638-75919657 None:intergenic 25.0%
! TACATATATGTTGTGTGTTA+TGG + chr2_1:75920230-75920249 Msa0225780:intron 25.0%
! ACATATATGTTGTGTGTTAT+GGG + chr2_1:75920231-75920250 Msa0225780:intron 25.0%
! CTGCAATAATTAATGATAGT+TGG - chr2_1:75920280-75920299 None:intergenic 25.0%
! CAACTATCATTAATTATTGC+AGG + chr2_1:75920278-75920297 Msa0225780:intron 25.0%
!! TCATGGATTCTAGAAAAAAT+AGG + chr2_1:75920327-75920346 Msa0225780:CDS 25.0%
! TATATATGAACTTACTTCCA+TGG - chr2_1:75920413-75920432 None:intergenic 25.0%
! AAATAATAACTAGCTGTAGA+TGG - chr2_1:75920446-75920465 None:intergenic 25.0%
!!! TTTTTTTCTTTCTTTCCTCT+AGG + chr2_1:75920595-75920614 Msa0225780:intron 25.0%
! AACAATATTAGTAAATCAGG+GGG - chr2_1:75920770-75920789 None:intergenic 25.0%
! CAACAATATTAGTAAATCAG+GGG - chr2_1:75920771-75920790 None:intergenic 25.0%
! GCAACAATATTAGTAAATCA+GGG - chr2_1:75920772-75920791 None:intergenic 25.0%
! TGCAACAATATTAGTAAATC+AGG - chr2_1:75920773-75920792 None:intergenic 25.0%
! TGATTTACTAATATTGTTGC+AGG + chr2_1:75920772-75920791 Msa0225780:intron 25.0%
! AAAAGACTCTAGCTATAAAA+TGG + chr2_1:75920965-75920984 Msa0225780:three_prime_UTR 25.0%
! CAAGATAAAATGAACAATAG+AGG + chr2_1:75921035-75921054 Msa0225780:three_prime_UTR 25.0%
! AGGTATATATACATAAGAGA+GGG + chr2_1:75921087-75921106 Msa0225780:three_prime_UTR 25.0%
!!! TGAGAATGATTAGTTTCTAT+TGG - chr2_1:75921195-75921214 None:intergenic 25.0%
CGTTCAAAGTTGTGTATATT+AGG - chr2_1:75919532-75919551 None:intergenic 30.0%
GTTCTTACTTTGATGTTGTT+GGG - chr2_1:75919837-75919856 None:intergenic 30.0%
ACTAGCTAGCTATATATGTA+CGG - chr2_1:75919859-75919878 None:intergenic 30.0%
!! AGATCCACTTTTCTTAGATA+TGG - chr2_1:75920057-75920076 None:intergenic 30.0%
GATGAATGAGAAGAAATTCA+TGG + chr2_1:75920310-75920329 Msa0225780:CDS 30.0%
! TGTTTGTTGAATCATGTAAG+AGG + chr2_1:75920619-75920638 Msa0225780:CDS 30.0%
TTGAATCATGTAAGAGGATA+AGG + chr2_1:75920625-75920644 Msa0225780:CDS 30.0%
!!! AGGACAATATTGATTTTGGT+TGG + chr2_1:75920664-75920683 Msa0225780:intron 30.0%
ATAATTATTCTTCTCACTGG+AGG - chr2_1:75920812-75920831 None:intergenic 30.0%
GCTATAATTATTCTTCTCAC+TGG - chr2_1:75920815-75920834 None:intergenic 30.0%
ATATAGCGATGATCATAATG+TGG - chr2_1:75920945-75920964 None:intergenic 30.0%
! CTTTGAGAAATGATTACATC+AGG + chr2_1:75921067-75921086 Msa0225780:three_prime_UTR 30.0%
CAGGTATATATACATAAGAG+AGG + chr2_1:75921086-75921105 Msa0225780:three_prime_UTR 30.0%
CGTTCAAAGTTGTGTATATT+AGG - chr2_1:75919532-75919551 None:intergenic 30.0%
GTTCTTACTTTGATGTTGTT+GGG - chr2_1:75919837-75919856 None:intergenic 30.0%
ACTAGCTAGCTATATATGTA+CGG - chr2_1:75919859-75919878 None:intergenic 30.0%
!! AGATCCACTTTTCTTAGATA+TGG - chr2_1:75920057-75920076 None:intergenic 30.0%
GATGAATGAGAAGAAATTCA+TGG + chr2_1:75920310-75920329 Msa0225780:CDS 30.0%
! TGTTTGTTGAATCATGTAAG+AGG + chr2_1:75920619-75920638 Msa0225780:CDS 30.0%
TTGAATCATGTAAGAGGATA+AGG + chr2_1:75920625-75920644 Msa0225780:CDS 30.0%
!!! AGGACAATATTGATTTTGGT+TGG + chr2_1:75920664-75920683 Msa0225780:intron 30.0%
ATAATTATTCTTCTCACTGG+AGG - chr2_1:75920812-75920831 None:intergenic 30.0%
GCTATAATTATTCTTCTCAC+TGG - chr2_1:75920815-75920834 None:intergenic 30.0%
ATATAGCGATGATCATAATG+TGG - chr2_1:75920945-75920964 None:intergenic 30.0%
! CTTTGAGAAATGATTACATC+AGG + chr2_1:75921067-75921086 Msa0225780:three_prime_UTR 30.0%
CAGGTATATATACATAAGAG+AGG + chr2_1:75921086-75921105 Msa0225780:three_prime_UTR 30.0%
ACAATGTCATCACAAATGAG+TGG + chr2_1:75919595-75919614 Msa0225780:exon 35.0%
TGGCAATTAGATGAAGATCA+AGG - chr2_1:75919753-75919772 None:intergenic 35.0%
!! ATGAGAAGTACTAGAAGTTG+AGG - chr2_1:75919776-75919795 None:intergenic 35.0%
TGATGTTGTTGGGAATTTAG+TGG - chr2_1:75919827-75919846 None:intergenic 35.0%
GGTTCTTACTTTGATGTTGT+TGG - chr2_1:75919838-75919857 None:intergenic 35.0%
ATATAGCTAGCTAGTTAGCT+AGG + chr2_1:75919864-75919883 Msa0225780:intron 35.0%
AGGGCAAGTAGGAAAAATAT+TGG + chr2_1:75919991-75920010 Msa0225780:CDS 35.0%
AGCTCCATATCTAAGAAAAG+TGG + chr2_1:75920050-75920069 Msa0225780:CDS 35.0%
GAAGCAATTAGTAGCAAACA+TGG - chr2_1:75920124-75920143 None:intergenic 35.0%
GCTACATACGAAGATAAAGA+TGG + chr2_1:75920362-75920381 Msa0225780:CDS 35.0%
TGATTCAACAAACATCCTAG+AGG - chr2_1:75920613-75920632 None:intergenic 35.0%
! GATAAGGCTTATGATTAGCT+CGG + chr2_1:75920641-75920660 Msa0225780:CDS 35.0%
!!! TCGGAGGACAATATTGATTT+TGG + chr2_1:75920660-75920679 Msa0225780:CDS 35.0%
ACTCTAGCTATAAAATGGAC+CGG + chr2_1:75920970-75920989 Msa0225780:three_prime_UTR 35.0%
GAGCAAGTAATTGTCCATTA+GGG - chr2_1:75921153-75921172 None:intergenic 35.0%
TGAGCAAGTAATTGTCCATT+AGG - chr2_1:75921154-75921173 None:intergenic 35.0%
ACAATGTCATCACAAATGAG+TGG + chr2_1:75919595-75919614 Msa0225780:exon 35.0%
TGGCAATTAGATGAAGATCA+AGG - chr2_1:75919753-75919772 None:intergenic 35.0%
!! ATGAGAAGTACTAGAAGTTG+AGG - chr2_1:75919776-75919795 None:intergenic 35.0%
TGATGTTGTTGGGAATTTAG+TGG - chr2_1:75919827-75919846 None:intergenic 35.0%
GGTTCTTACTTTGATGTTGT+TGG - chr2_1:75919838-75919857 None:intergenic 35.0%
ATATAGCTAGCTAGTTAGCT+AGG + chr2_1:75919864-75919883 Msa0225780:intron 35.0%
AGGGCAAGTAGGAAAAATAT+TGG + chr2_1:75919991-75920010 Msa0225780:CDS 35.0%
AGCTCCATATCTAAGAAAAG+TGG + chr2_1:75920050-75920069 Msa0225780:CDS 35.0%
GAAGCAATTAGTAGCAAACA+TGG - chr2_1:75920124-75920143 None:intergenic 35.0%
GCTACATACGAAGATAAAGA+TGG + chr2_1:75920362-75920381 Msa0225780:CDS 35.0%
TGATTCAACAAACATCCTAG+AGG - chr2_1:75920613-75920632 None:intergenic 35.0%
! GATAAGGCTTATGATTAGCT+CGG + chr2_1:75920641-75920660 Msa0225780:CDS 35.0%
!!! TCGGAGGACAATATTGATTT+TGG + chr2_1:75920660-75920679 Msa0225780:CDS 35.0%
ACTCTAGCTATAAAATGGAC+CGG + chr2_1:75920970-75920989 Msa0225780:three_prime_UTR 35.0%
GAGCAAGTAATTGTCCATTA+GGG - chr2_1:75921153-75921172 None:intergenic 35.0%
TGAGCAAGTAATTGTCCATT+AGG - chr2_1:75921154-75921173 None:intergenic 35.0%
TCGTACATTCAATGGAACTG+TGG - chr2_1:75919630-75919649 None:intergenic 40.0%
!! TGCAGTTTTGGTACTACTAC+CGG - chr2_1:75919701-75919720 None:intergenic 40.0%
! ACGTTTTATGCCTGCAGTTT+TGG - chr2_1:75919713-75919732 None:intergenic 40.0%
!! AGAAGTACTAGAAGTTGAGG+TGG - chr2_1:75919773-75919792 None:intergenic 40.0%
TTGCAAGTACGTGAAAGTTG+CGG + chr2_1:75920020-75920039 Msa0225780:CDS 40.0%
ACGAAGATAAAGATGGTGAC+TGG + chr2_1:75920369-75920388 Msa0225780:CDS 40.0%
TGGAGCTTGTACACTTTGAA+AGG - chr2_1:75920900-75920919 None:intergenic 40.0%
CTCTAGCTATAAAATGGACC+GGG + chr2_1:75920971-75920990 Msa0225780:three_prime_UTR 40.0%
CTATAAAATGGACCGGGTTT+GGG + chr2_1:75920977-75920996 Msa0225780:three_prime_UTR 40.0%
TCGTACATTCAATGGAACTG+TGG - chr2_1:75919630-75919649 None:intergenic 40.0%
!! TGCAGTTTTGGTACTACTAC+CGG - chr2_1:75919701-75919720 None:intergenic 40.0%
! ACGTTTTATGCCTGCAGTTT+TGG - chr2_1:75919713-75919732 None:intergenic 40.0%
!! AGAAGTACTAGAAGTTGAGG+TGG - chr2_1:75919773-75919792 None:intergenic 40.0%
TTGCAAGTACGTGAAAGTTG+CGG + chr2_1:75920020-75920039 Msa0225780:CDS 40.0%
ACGAAGATAAAGATGGTGAC+TGG + chr2_1:75920369-75920388 Msa0225780:CDS 40.0%
TGGAGCTTGTACACTTTGAA+AGG - chr2_1:75920900-75920919 None:intergenic 40.0%
CTCTAGCTATAAAATGGACC+GGG + chr2_1:75920971-75920990 Msa0225780:three_prime_UTR 40.0%
CTATAAAATGGACCGGGTTT+GGG + chr2_1:75920977-75920996 Msa0225780:three_prime_UTR 40.0%
!! GCAGTTTTGGTACTACTACC+GGG - chr2_1:75919700-75919719 None:intergenic 45.0%
GGTAGTAGTACCAAAACTGC+AGG + chr2_1:75919700-75919719 Msa0225780:CDS 45.0%
ATGTAAAGTTGGGTGCATGC+AGG + chr2_1:75919941-75919960 Msa0225780:intron 45.0%
TGTAAAGTTGGGTGCATGCA+GGG + chr2_1:75919942-75919961 Msa0225780:intron 45.0%
! TTTTTCCTACTTGCCCTCAC+CGG - chr2_1:75919988-75920007 None:intergenic 45.0%
TACGTGAAAGTTGCGGTTGA+TGG + chr2_1:75920027-75920046 Msa0225780:CDS 45.0%
! ATGGTGACTGGATGTTGCTT+GGG + chr2_1:75920381-75920400 Msa0225780:CDS 45.0%
! AAGGCTTATGATTAGCTCGG+AGG + chr2_1:75920644-75920663 Msa0225780:CDS 45.0%
TGTTGCAGGTTCCAGATGTA+CGG + chr2_1:75920786-75920805 Msa0225780:intron 45.0%
GCTATAAAATGGACCGGGTT+TGG + chr2_1:75920976-75920995 Msa0225780:three_prime_UTR 45.0%
AACCAGGGGTGGTCTTTATT+AGG + chr2_1:75921009-75921028 Msa0225780:three_prime_UTR 45.0%
TTCCTAATAAAGACCACCCC+TGG - chr2_1:75921014-75921033 None:intergenic 45.0%
!! GCAGTTTTGGTACTACTACC+GGG - chr2_1:75919700-75919719 None:intergenic 45.0%
GGTAGTAGTACCAAAACTGC+AGG + chr2_1:75919700-75919719 Msa0225780:CDS 45.0%
ATGTAAAGTTGGGTGCATGC+AGG + chr2_1:75919941-75919960 Msa0225780:intron 45.0%
TGTAAAGTTGGGTGCATGCA+GGG + chr2_1:75919942-75919961 Msa0225780:intron 45.0%
! TTTTTCCTACTTGCCCTCAC+CGG - chr2_1:75919988-75920007 None:intergenic 45.0%
TACGTGAAAGTTGCGGTTGA+TGG + chr2_1:75920027-75920046 Msa0225780:CDS 45.0%
! ATGGTGACTGGATGTTGCTT+GGG + chr2_1:75920381-75920400 Msa0225780:CDS 45.0%
! AAGGCTTATGATTAGCTCGG+AGG + chr2_1:75920644-75920663 Msa0225780:CDS 45.0%
TGTTGCAGGTTCCAGATGTA+CGG + chr2_1:75920786-75920805 Msa0225780:intron 45.0%
GCTATAAAATGGACCGGGTT+TGG + chr2_1:75920976-75920995 Msa0225780:three_prime_UTR 45.0%
AACCAGGGGTGGTCTTTATT+AGG + chr2_1:75921009-75921028 Msa0225780:three_prime_UTR 45.0%
TTCCTAATAAAGACCACCCC+TGG - chr2_1:75921014-75921033 None:intergenic 45.0%
ACTACTACCGGGAAGACCAA+GGG - chr2_1:75919689-75919708 None:intergenic 50.0%
TACTACTACCGGGAAGACCA+AGG - chr2_1:75919690-75919709 None:intergenic 50.0%
GATGGTGACTGGATGTTGCT+TGG + chr2_1:75920380-75920399 Msa0225780:CDS 50.0%
! TGGTGACTGGATGTTGCTTG+GGG + chr2_1:75920382-75920401 Msa0225780:CDS 50.0%
TTTGGGTGCAGTTGGAACCA+GGG + chr2_1:75920994-75921013 Msa0225780:three_prime_UTR 50.0%
ACTACTACCGGGAAGACCAA+GGG - chr2_1:75919689-75919708 None:intergenic 50.0%
TACTACTACCGGGAAGACCA+AGG - chr2_1:75919690-75919709 None:intergenic 50.0%
GATGGTGACTGGATGTTGCT+TGG + chr2_1:75920380-75920399 Msa0225780:CDS 50.0%
! TGGTGACTGGATGTTGCTTG+GGG + chr2_1:75920382-75920401 Msa0225780:CDS 50.0%
TTTGGGTGCAGTTGGAACCA+GGG + chr2_1:75920994-75921013 Msa0225780:three_prime_UTR 50.0%
CAGGAGACTGAGCTTACCCT+TGG + chr2_1:75919670-75919689 Msa0225780:CDS 55.0%
! GAGCTTACCCTTGGTCTTCC+CGG + chr2_1:75919679-75919698 Msa0225780:CDS 55.0%
TGCATGCAGGGAACATGTGG+TGG + chr2_1:75919954-75919973 Msa0225780:intron 55.0%
GCATGCAGGGAACATGTGGT+GGG + chr2_1:75919955-75919974 Msa0225780:intron 55.0%
! TGTTGCTTGGGGATGCACCA+TGG + chr2_1:75920393-75920412 Msa0225780:CDS 55.0%
GTTCCAACTGCACCCAAACC+CGG - chr2_1:75920992-75921011 None:intergenic 55.0%
GTTTGGGTGCAGTTGGAACC+AGG + chr2_1:75920993-75921012 Msa0225780:three_prime_UTR 55.0%
TTGGGTGCAGTTGGAACCAG+GGG + chr2_1:75920995-75921014 Msa0225780:three_prime_UTR 55.0%
CAGGAGACTGAGCTTACCCT+TGG + chr2_1:75919670-75919689 Msa0225780:CDS 55.0%
! GAGCTTACCCTTGGTCTTCC+CGG + chr2_1:75919679-75919698 Msa0225780:CDS 55.0%
TGCATGCAGGGAACATGTGG+TGG + chr2_1:75919954-75919973 Msa0225780:intron 55.0%
GCATGCAGGGAACATGTGGT+GGG + chr2_1:75919955-75919974 Msa0225780:intron 55.0%
! TGTTGCTTGGGGATGCACCA+TGG + chr2_1:75920393-75920412 Msa0225780:CDS 55.0%
GTTCCAACTGCACCCAAACC+CGG - chr2_1:75920992-75921011 None:intergenic 55.0%
GTTTGGGTGCAGTTGGAACC+AGG + chr2_1:75920993-75921012 Msa0225780:three_prime_UTR 55.0%
TTGGGTGCAGTTGGAACCAG+GGG + chr2_1:75920995-75921014 Msa0225780:three_prime_UTR 55.0%
GGGTGCATGCAGGGAACATG+TGG + chr2_1:75919951-75919970 Msa0225780:intron 60.0%
! GCAGGGAACATGTGGTGGGA+TGG + chr2_1:75919959-75919978 Msa0225780:intron 60.0%
! ACATGTGGTGGGATGGCCAC+CGG + chr2_1:75919966-75919985 Msa0225780:CDS 60.0%
TTCCTACTTGCCCTCACCGG+TGG - chr2_1:75919985-75920004 None:intergenic 60.0%
CTCACTGGAGGCCGTACATC+TGG - chr2_1:75920800-75920819 None:intergenic 60.0%
GGGTGCATGCAGGGAACATG+TGG + chr2_1:75919951-75919970 Msa0225780:intron 60.0%
! GCAGGGAACATGTGGTGGGA+TGG + chr2_1:75919959-75919978 Msa0225780:intron 60.0%
! ACATGTGGTGGGATGGCCAC+CGG + chr2_1:75919966-75919985 Msa0225780:CDS 60.0%
TTCCTACTTGCCCTCACCGG+TGG - chr2_1:75919985-75920004 None:intergenic 60.0%
CTCACTGGAGGCCGTACATC+TGG - chr2_1:75920800-75920819 None:intergenic 60.0%
GGACCGGGTTTGGGTGCAGT+TGG + chr2_1:75920986-75921005 Msa0225780:three_prime_UTR 65.0%
GGTGCAGTTGGAACCAGGGG+TGG + chr2_1:75920998-75921017 Msa0225780:three_prime_UTR 65.0%
GGACCGGGTTTGGGTGCAGT+TGG + chr2_1:75920986-75921005 Msa0225780:three_prime_UTR 65.0%
GGTGCAGTTGGAACCAGGGG+TGG + chr2_1:75920998-75921017 Msa0225780:three_prime_UTR 65.0%
TGGTGGGATGGCCACCGGTG+AGG + chr2_1:75919971-75919990 Msa0225780:CDS 70.0%
GGTGGGATGGCCACCGGTGA+GGG + chr2_1:75919972-75919991 Msa0225780:CDS 70.0%
GGCCACCGGTGAGGGCAAGT+AGG + chr2_1:75919980-75919999 Msa0225780:CDS 70.0%
TGGTGGGATGGCCACCGGTG+AGG + chr2_1:75919971-75919990 Msa0225780:CDS 70.0%
GGTGGGATGGCCACCGGTGA+GGG + chr2_1:75919972-75919991 Msa0225780:CDS 70.0%
GGCCACCGGTGAGGGCAAGT+AGG + chr2_1:75919980-75919999 Msa0225780:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75919520 75921239 75919520 ID=Msa0225780;Name=Msa0225780
chr2_1 mRNA 75919520 75921239 75919520 ID=Msa0225780-mRNA-1;Parent=Msa0225780;Name=Msa0225780-mRNA-1;_AED=0.05;_eAED=0.05;_QI=78|1|1|1|1|1|5|402|221
chr2_1 exon 75919520 75919848 75919520 ID=Msa0225780-mRNA-1:exon:22835;Parent=Msa0225780-mRNA-1
chr2_1 exon 75919963 75920150 75919963 ID=Msa0225780-mRNA-1:exon:22836;Parent=Msa0225780-mRNA-1
chr2_1 exon 75920300 75920417 75920300 ID=Msa0225780-mRNA-1:exon:22837;Parent=Msa0225780-mRNA-1
chr2_1 exon 75920617 75920681 75920617 ID=Msa0225780-mRNA-1:exon:22838;Parent=Msa0225780-mRNA-1
chr2_1 exon 75920794 75921239 75920794 ID=Msa0225780-mRNA-1:exon:22839;Parent=Msa0225780-mRNA-1
chr2_1 five_prime_UTR 75919520 75919597 75919520 ID=Msa0225780-mRNA-1:five_prime_utr;Parent=Msa0225780-mRNA-1
chr2_1 CDS 75919598 75919848 75919598 ID=Msa0225780-mRNA-1:cds;Parent=Msa0225780-mRNA-1
chr2_1 CDS 75919963 75920150 75919963 ID=Msa0225780-mRNA-1:cds;Parent=Msa0225780-mRNA-1
chr2_1 CDS 75920300 75920417 75920300 ID=Msa0225780-mRNA-1:cds;Parent=Msa0225780-mRNA-1
chr2_1 CDS 75920617 75920681 75920617 ID=Msa0225780-mRNA-1:cds;Parent=Msa0225780-mRNA-1
chr2_1 CDS 75920794 75920837 75920794 ID=Msa0225780-mRNA-1:cds;Parent=Msa0225780-mRNA-1
chr2_1 three_prime_UTR 75920838 75921239 75920838 ID=Msa0225780-mRNA-1:three_prime_utr;Parent=Msa0225780-mRNA-1
Gene Sequence

>Msa0225780

ATGTCATCACAAATGAGTGGTAGCAACTTCCACAGTTCCATTGAATGTACGAATAATAAGATGAATATTATTCAGGAGACTGAGCTTACCCTTGGTCTTCCCGGTAGTAGTACCAAAACTGCAGGCATAAAACGTAGCTTCTCTCACACTCACCTTGATCTTCATCTAATTGCCACCTCAACTTCTAGTACTTCTCATCATCATAATTCACTAAATTCTAATAATACCACTAAATTCCCAACAACATCAAAGGAACATGTGGTGGGATGGCCACCGGTGAGGGCAAGTAGGAAAAATATTGGTAGTAGTTGCAAGTACGTGAAAGTTGCGGTTGATGGAGCTCCATATCTAAGAAAAGTGGATCTTGAAGTTTATGAAAGTTATGATCATCTCTTCACTGCATTGAATACCATGTTTGCTACTAATTGCTTCAATATACGTAATGATTTGATGAATGAGAAGAAATTCATGGATTCTAGAAAAAATAGGAATGAATACATGGCTACATACGAAGATAAAGATGGTGACTGGATGTTGCTTGGGGATGCACCATGGAAGATGTTTGTTGAATCATGTAAGAGGATAAGGCTTATGATTAGCTCGGAGGACAATATTGATTTTGGTTCCAGATGTACGGCCTCCAGTGAGAAGAATAATTATAGCTAG
Protein sequence

>Msa0225780

MSSQMSGSNFHSSIECTNNKMNIIQETELTLGLPGSSTKTAGIKRSFSHTHLDLHLIATSTSSTSHHHNSLNSNNTTKFPTTSKEHVVGWPPVRASRKNIGSSCKYVKVAVDGAPYLRKVDLEVYESYDHLFTALNTMFATNCFNIRNDLMNEKKFMDSRKNRNEYMATYEDKDGDWMLLGDAPWKMFVESCKRIRLMISSEDNIDFGSRCTASSEKNNYS*