AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0253220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0253220 MtrunA17_Chr1g0204101 53.922 102 33 3 60 149 213 312 1.94e-24 96.3
Msa0253220 MtrunA17_Chr1g0204101 67.647 68 21 1 1 68 18 84 4.46e-21 87.4
Msa0253220 MtrunA17_Chr6g0474331 61.333 75 28 1 3 77 24 97 4.92e-21 87.4
Msa0253220 MtrunA17_Chr6g0474331 51.961 102 35 3 60 149 218 317 6.15e-21 87.0
Msa0253220 MtrunA17_Chr5g0434971 63.380 71 25 1 5 75 65 134 1.81e-19 83.2
Msa0253220 MtrunA17_Chr5g0434971 44.118 102 44 3 60 150 257 356 8.96e-18 78.6
Msa0253220 MtrunA17_Chr1g0177771 51.042 96 35 2 60 143 429 524 7.95e-19 82.0
Msa0253220 MtrunA17_Chr1g0177321 50.000 96 36 2 60 143 346 441 2.23e-18 80.5
Msa0253220 MtrunA17_Chr1g0171921 51.042 96 35 2 60 143 372 467 2.27e-18 80.5
Msa0253220 MtrunA17_Chr1g0183241 49.020 102 38 4 60 149 279 378 1.82e-17 77.8
Msa0253220 MtrunA17_Chr2g0329451 60.656 61 24 0 5 65 12 72 1.73e-16 74.7
Msa0253220 MtrunA17_Chr2g0329451 40.594 101 48 3 60 150 204 302 1.07e-14 69.7
Msa0253220 MtrunA17_Chr4g0033631 62.295 61 23 0 5 65 13 73 1.88e-16 74.3
Msa0253220 MtrunA17_Chr4g0033631 41.584 101 47 3 60 150 205 303 2.28e-14 68.6
Msa0253220 MtrunA17_Chr1g0183661 48.039 102 32 5 60 149 173 265 5.14e-15 70.5
Msa0253220 MtrunA17_Chr1g0188291 40.594 101 48 3 60 150 198 296 1.38e-13 66.6
Msa0253220 MtrunA17_Chr1g0188291 55.932 59 26 0 7 65 8 66 4.93e-13 65.1
Msa0253220 MtrunA17_Chr7g0246411 50.847 59 29 0 7 65 41 99 1.75e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0253220 AT5G51830.2 67.188 64 21 0 2 65 18 81 5.61e-20 84.7
Msa0253220 AT5G51830.2 46.602 103 41 3 60 150 213 313 2.96e-19 82.8
Msa0253220 AT5G51830.1 67.188 64 21 0 2 65 18 81 5.61e-20 84.7
Msa0253220 AT5G51830.1 46.602 103 41 3 60 150 213 313 2.96e-19 82.8
Msa0253220 AT1G66430.1 47.059 102 41 3 60 150 255 354 2.55e-19 83.2
Msa0253220 AT1G66430.1 60.606 66 26 0 3 68 61 126 7.61e-18 79.0
Msa0253220 AT3G59480.1 62.712 59 22 0 7 65 10 68 6.76e-16 73.2
Msa0253220 AT3G59480.1 36.634 101 52 3 60 150 200 298 1.24e-11 61.2
Msa0253220 AT1G06020.1 60.000 60 24 0 6 65 9 68 1.09e-15 72.8
Msa0253220 AT1G06020.1 36.634 101 52 3 60 150 200 298 1.78e-11 60.8
Msa0253220 AT2G31390.1 61.667 60 23 0 6 65 8 67 1.65e-15 72.4
Msa0253220 AT2G31390.1 36.634 101 52 3 60 150 199 297 4.83e-12 62.4
Msa0253220 AT4G10260.1 41.584 101 47 3 60 150 196 294 3.80e-14 68.6
Msa0253220 AT4G10260.1 54.237 59 27 0 7 65 6 64 2.98e-12 63.2
Msa0253220 AT1G06030.1 53.968 63 29 0 3 65 7 69 1.98e-13 66.6
Msa0253220 AT1G06030.1 38.614 101 50 3 60 150 201 299 2.30e-13 66.2

Find 47 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTGAAAAGGGTATAAT+TGG 0.186965 2_2:-44861478 None:intergenic
CTCTTGCCACACCCACTTAA+TGG 0.203102 2_2:+44861505 Msa0253220:three_prime_UTR
TATTTCGAATGTTCCCATTA+TGG 0.239655 2_2:+44861343 Msa0253220:three_prime_UTR
AAGCCACCTGCCACGTTTGC+AGG 0.280795 2_2:-44860203 None:intergenic
AGTGGATTGGTTGTTCGTTT+TGG 0.323188 2_2:+44860095 Msa0253220:CDS
AAAGTATTGCCTTTATCAAT+AGG 0.325737 2_2:-44861428 None:intergenic
TCTTGCCACACCCACTTAAT+GGG 0.332750 2_2:+44861506 Msa0253220:three_prime_UTR
ATGGTGATGCATTTAATTAT+AGG 0.334354 2_2:+44861043 Msa0253220:CDS
GCAGGTGGCTTCTCTGAGTC+GGG 0.335070 2_2:+44860215 Msa0253220:CDS
TAATTATAATAAATCCATAA+TGG 0.348719 2_2:-44861357 None:intergenic
CGAATTACCGTGCAAATACT+TGG 0.356808 2_2:-44861090 None:intergenic
GGCAGGTGGCTTCTCTGAGT+CGG 0.375859 2_2:+44860214 Msa0253220:CDS
TCTTCTATACCTATTGATAA+AGG 0.382200 2_2:+44861419 Msa0253220:three_prime_UTR
AATTATAATAAATCCATAAT+GGG 0.398159 2_2:-44861356 None:intergenic
GTAAATGCCACCCCATTAAG+TGG 0.465511 2_2:-44861517 None:intergenic
CTTGCAATTTGATGCAACAT+AGG 0.466474 2_2:+44861281 Msa0253220:three_prime_UTR
ATGCTTCTAATTGTTACTGA+AGG 0.481972 2_2:+44860732 Msa0253220:CDS
ATTAGAGGGGTTCACACTAC+TGG 0.492764 2_2:+44861018 Msa0253220:CDS
GGTGCTCCTGCAAACGTGGC+AGG 0.499722 2_2:+44860197 Msa0253220:CDS
AGGTGGCTTCTCTGAGTCGG+GGG 0.500865 2_2:+44860217 Msa0253220:CDS
CCAGGAGCTTTCTTGAAAGC+AGG 0.501737 2_2:-44860173 None:intergenic
CCTGCTTTCAAGAAAGCTCC+TGG 0.520107 2_2:+44860173 Msa0253220:CDS
CAGGTGGCTTCTCTGAGTCG+GGG 0.541084 2_2:+44860216 Msa0253220:CDS
CTGCAAAGAAACAAGTGGAT+TGG 0.541361 2_2:+44860082 None:intergenic
TGCTTCTAATTGTTACTGAA+GGG 0.552682 2_2:+44860733 Msa0253220:CDS
GCCACCCCATTAAGTGGGTG+TGG 0.554671 2_2:-44861511 None:intergenic
AGAGACACTGCACCCACTGT+TGG 0.557623 2_2:-44860140 None:intergenic
AGGGATTGCTCCTCATGCAT+TGG 0.564760 2_2:-44861235 None:intergenic
ATTGTTACTGAAGGGTCAGA+GGG 0.569886 2_2:+44860741 Msa0253220:CDS
AATTGTTACTGAAGGGTCAG+AGG 0.570981 2_2:+44860740 Msa0253220:CDS
TTCTGACCCAAGTATTTGCA+CGG 0.577534 2_2:+44861083 Msa0253220:CDS
TGGTGGTGCTCCTGCAAACG+TGG 0.587504 2_2:+44860193 Msa0253220:CDS
ACGTTTGCAGGAGCACCACC+AGG 0.595374 2_2:-44860191 None:intergenic
GCTCCTGCAAACGTGGCAGG+TGG 0.602455 2_2:+44860200 Msa0253220:CDS
GGTGGCTTCTCTGAGTCGGG+GGG 0.606297 2_2:+44860218 Msa0253220:CDS
GCCACACCCACTTAATGGGG+TGG 0.606948 2_2:+44861510 Msa0253220:three_prime_UTR
TTCATCTGCATCTAGAAGCA+AGG 0.610483 2_2:+44860241 Msa0253220:CDS
GGGGTTCACACTACTGGTGA+TGG 0.616600 2_2:+44861024 Msa0253220:CDS
CTTGCCACACCCACTTAATG+GGG 0.618518 2_2:+44861507 Msa0253220:three_prime_UTR
GAATTACCGTGCAAATACTT+GGG 0.623480 2_2:-44861089 None:intergenic
TTTCTTGAAAGCAGGCGCAT+CGG 0.624453 2_2:-44860165 None:intergenic
GTATAATTGGAAATAAACAC+AGG 0.629840 2_2:-44861465 None:intergenic
GAAGGCTGCAAAGAAACAAG+TGG 0.629890 2_2:+44860077 None:intergenic
TAAATGCCACCCCATTAAGT+GGG 0.646618 2_2:-44861516 None:intergenic
GCTTTCAAGAAAGCTCCTGG+TGG 0.650698 2_2:+44860176 Msa0253220:CDS
TTATAGGATTCTCTACAGCG+TGG 0.718727 2_2:+44861059 Msa0253220:CDS
GGGCTTTATATATCACACCA+AGG 0.729307 2_2:+44860761 Msa0253220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAATAAATCCATAAT+GGG - chr2_2:44861359-44861378 None:intergenic 10.0%
!! TAATTATAATAAATCCATAA+TGG - chr2_2:44861360-44861379 None:intergenic 10.0%
!! ATTGTCAAAAAAAAAAAAAT+AGG - chr2_2:44861394-44861413 None:intergenic 10.0%
!! AATTATAATAAATCCATAAT+GGG - chr2_2:44861359-44861378 None:intergenic 10.0%
!! TAATTATAATAAATCCATAA+TGG - chr2_2:44861360-44861379 None:intergenic 10.0%
!! ATTGTCAAAAAAAAAAAAAT+AGG - chr2_2:44861394-44861413 None:intergenic 10.0%
!!! TTTAATTTAATACTGATTGC+AGG + chr2_2:44860359-44860378 Msa0253220:intron 20.0%
!! AAAATCATATTACGTACATA+TGG + chr2_2:44860499-44860518 Msa0253220:intron 20.0%
!! AAATCATATTACGTACATAT+GGG + chr2_2:44860500-44860519 Msa0253220:intron 20.0%
!! AAAAGTTACCTTTATGATAT+TGG - chr2_2:44860532-44860551 None:intergenic 20.0%
!!! GTAAAGATGATATTACTTTT+TGG + chr2_2:44860667-44860686 Msa0253220:CDS 20.0%
!!! AAACTTTTTTGAAAACTTCT+CGG - chr2_2:44861212-44861231 None:intergenic 20.0%
!!! AAGAGTTTTAAAAGTGAAAA+GGG - chr2_2:44861490-44861509 None:intergenic 20.0%
!!! TTTAATTTAATACTGATTGC+AGG + chr2_2:44860359-44860378 Msa0253220:intron 20.0%
!! AAAATCATATTACGTACATA+TGG + chr2_2:44860499-44860518 Msa0253220:intron 20.0%
!! AAATCATATTACGTACATAT+GGG + chr2_2:44860500-44860519 Msa0253220:intron 20.0%
!! AAAAGTTACCTTTATGATAT+TGG - chr2_2:44860532-44860551 None:intergenic 20.0%
!!! GTAAAGATGATATTACTTTT+TGG + chr2_2:44860667-44860686 Msa0253220:CDS 20.0%
!!! AAACTTTTTTGAAAACTTCT+CGG - chr2_2:44861212-44861231 None:intergenic 20.0%
!!! AAGAGTTTTAAAAGTGAAAA+GGG - chr2_2:44861490-44861509 None:intergenic 20.0%
!!! TCGTTTTGGTAAAATGTTAA+TGG + chr2_2:44860109-44860128 Msa0253220:CDS 25.0%
!! AATATGATTTTTAACAGCCT+TGG - chr2_2:44860490-44860509 None:intergenic 25.0%
!!! CAATTAGAAGCATGATTTTA+GGG - chr2_2:44860725-44860744 None:intergenic 25.0%
!!! ACAATTAGAAGCATGATTTT+AGG - chr2_2:44860726-44860745 None:intergenic 25.0%
!! TGATTACTAAATTTTTGTGC+AGG + chr2_2:44860981-44861000 Msa0253220:intron 25.0%
!! ATGGTGATGCATTTAATTAT+AGG + chr2_2:44861043-44861062 Msa0253220:CDS 25.0%
!!! GTTGATGAAGTTATTTTATG+TGG + chr2_2:44861169-44861188 Msa0253220:intron 25.0%
! TCTTCTATACCTATTGATAA+AGG + chr2_2:44861419-44861438 Msa0253220:three_prime_UTR 25.0%
!!! AAAGTATTGCCTTTATCAAT+AGG - chr2_2:44861431-44861450 None:intergenic 25.0%
!! AAAGGCAATACTTTTCTTTT+TGG + chr2_2:44861437-44861456 Msa0253220:three_prime_UTR 25.0%
! GTATAATTGGAAATAAACAC+AGG - chr2_2:44861468-44861487 None:intergenic 25.0%
!! AAAAGTGAAAAGGGTATAAT+TGG - chr2_2:44861481-44861500 None:intergenic 25.0%
!!! CAAGAGTTTTAAAAGTGAAA+AGG - chr2_2:44861491-44861510 None:intergenic 25.0%
!!! TCGTTTTGGTAAAATGTTAA+TGG + chr2_2:44860109-44860128 Msa0253220:CDS 25.0%
!! AATATGATTTTTAACAGCCT+TGG - chr2_2:44860490-44860509 None:intergenic 25.0%
!!! CAATTAGAAGCATGATTTTA+GGG - chr2_2:44860725-44860744 None:intergenic 25.0%
!!! ACAATTAGAAGCATGATTTT+AGG - chr2_2:44860726-44860745 None:intergenic 25.0%
!! TGATTACTAAATTTTTGTGC+AGG + chr2_2:44860981-44861000 Msa0253220:intron 25.0%
!! ATGGTGATGCATTTAATTAT+AGG + chr2_2:44861043-44861062 Msa0253220:CDS 25.0%
!!! GTTGATGAAGTTATTTTATG+TGG + chr2_2:44861169-44861188 Msa0253220:intron 25.0%
! TCTTCTATACCTATTGATAA+AGG + chr2_2:44861419-44861438 Msa0253220:three_prime_UTR 25.0%
!!! AAAGTATTGCCTTTATCAAT+AGG - chr2_2:44861431-44861450 None:intergenic 25.0%
!! AAAGGCAATACTTTTCTTTT+TGG + chr2_2:44861437-44861456 Msa0253220:three_prime_UTR 25.0%
! GTATAATTGGAAATAAACAC+AGG - chr2_2:44861468-44861487 None:intergenic 25.0%
!! AAAAGTGAAAAGGGTATAAT+TGG - chr2_2:44861481-44861500 None:intergenic 25.0%
!!! CAAGAGTTTTAAAAGTGAAA+AGG - chr2_2:44861491-44861510 None:intergenic 25.0%
! ATTATACAGGCTTTAACCAT+GGG - chr2_2:44860337-44860356 None:intergenic 30.0%
! TATTATACAGGCTTTAACCA+TGG - chr2_2:44860338-44860357 None:intergenic 30.0%
!!! GATGATATTACTTTTTGGAC+TGG + chr2_2:44860672-44860691 Msa0253220:CDS 30.0%
ATGCTTCTAATTGTTACTGA+AGG + chr2_2:44860732-44860751 Msa0253220:CDS 30.0%
TGCTTCTAATTGTTACTGAA+GGG + chr2_2:44860733-44860752 Msa0253220:CDS 30.0%
AAATCTGAGTGAAATGACTT+TGG - chr2_2:44860795-44860814 None:intergenic 30.0%
!!! CAGATTTTAATTGTCTGCAT+TGG + chr2_2:44860808-44860827 Msa0253220:intron 30.0%
! ATAATCGTTGACTGAAATGT+TGG + chr2_2:44860835-44860854 Msa0253220:intron 30.0%
AATTTAGTAATCATCACTGC+TGG - chr2_2:44860974-44860993 None:intergenic 30.0%
! AATTTTTGTGCAGGATTTTG+CGG + chr2_2:44860990-44861009 Msa0253220:intron 30.0%
AAAAGATAGTTGTGAGTGTT+TGG + chr2_2:44861145-44861164 Msa0253220:intron 30.0%
TATTTCGAATGTTCCCATTA+TGG + chr2_2:44861343-44861362 Msa0253220:three_prime_UTR 30.0%
! ATTATACAGGCTTTAACCAT+GGG - chr2_2:44860337-44860356 None:intergenic 30.0%
! TATTATACAGGCTTTAACCA+TGG - chr2_2:44860338-44860357 None:intergenic 30.0%
!!! GATGATATTACTTTTTGGAC+TGG + chr2_2:44860672-44860691 Msa0253220:CDS 30.0%
ATGCTTCTAATTGTTACTGA+AGG + chr2_2:44860732-44860751 Msa0253220:CDS 30.0%
TGCTTCTAATTGTTACTGAA+GGG + chr2_2:44860733-44860752 Msa0253220:CDS 30.0%
AAATCTGAGTGAAATGACTT+TGG - chr2_2:44860795-44860814 None:intergenic 30.0%
!!! CAGATTTTAATTGTCTGCAT+TGG + chr2_2:44860808-44860827 Msa0253220:intron 30.0%
! ATAATCGTTGACTGAAATGT+TGG + chr2_2:44860835-44860854 Msa0253220:intron 30.0%
AATTTAGTAATCATCACTGC+TGG - chr2_2:44860974-44860993 None:intergenic 30.0%
! AATTTTTGTGCAGGATTTTG+CGG + chr2_2:44860990-44861009 Msa0253220:intron 30.0%
AAAAGATAGTTGTGAGTGTT+TGG + chr2_2:44861145-44861164 Msa0253220:intron 30.0%
TATTTCGAATGTTCCCATTA+TGG + chr2_2:44861343-44861362 Msa0253220:three_prime_UTR 30.0%
ATCTAGAAGCAAGGTTATGT+TGG + chr2_2:44860250-44860269 Msa0253220:intron 35.0%
AATGTGACCATAAAAACCCA+TGG + chr2_2:44860318-44860337 Msa0253220:intron 35.0%
! GCTTTAACCATGGGTTTTTA+TGG - chr2_2:44860328-44860347 None:intergenic 35.0%
! CAGGCTAAAATATTCCGTTT+TGG + chr2_2:44860378-44860397 Msa0253220:intron 35.0%
TCAAACTGATGGAACCAAAA+CGG - chr2_2:44860395-44860414 None:intergenic 35.0%
TGGTTCATCAATCAAACTGA+TGG - chr2_2:44860406-44860425 None:intergenic 35.0%
GGAACTAGCCAATATCATAA+AGG + chr2_2:44860521-44860540 Msa0253220:intron 35.0%
GAAGAAAGAATGATGTGCAA+TGG - chr2_2:44860601-44860620 None:intergenic 35.0%
!!! GATATTACTTTTTGGACTGG+TGG + chr2_2:44860675-44860694 Msa0253220:CDS 35.0%
! ATTTTTGTGCAGGATTTTGC+GGG + chr2_2:44860991-44861010 Msa0253220:intron 35.0%
GAATTACCGTGCAAATACTT+GGG - chr2_2:44861092-44861111 None:intergenic 35.0%
CAAGTATTTGCACGGTAATT+CGG + chr2_2:44861091-44861110 Msa0253220:intron 35.0%
! AAAGTTTTTGCCAATGCATG+AGG + chr2_2:44861225-44861244 Msa0253220:CDS 35.0%
! CATCTTTTGTAGGTAATGCA+GGG - chr2_2:44861257-44861276 None:intergenic 35.0%
CTTGCAATTTGATGCAACAT+AGG + chr2_2:44861281-44861300 Msa0253220:three_prime_UTR 35.0%
ATCTAGAAGCAAGGTTATGT+TGG + chr2_2:44860250-44860269 Msa0253220:intron 35.0%
AATGTGACCATAAAAACCCA+TGG + chr2_2:44860318-44860337 Msa0253220:intron 35.0%
! GCTTTAACCATGGGTTTTTA+TGG - chr2_2:44860328-44860347 None:intergenic 35.0%
! CAGGCTAAAATATTCCGTTT+TGG + chr2_2:44860378-44860397 Msa0253220:intron 35.0%
TCAAACTGATGGAACCAAAA+CGG - chr2_2:44860395-44860414 None:intergenic 35.0%
TGGTTCATCAATCAAACTGA+TGG - chr2_2:44860406-44860425 None:intergenic 35.0%
GGAACTAGCCAATATCATAA+AGG + chr2_2:44860521-44860540 Msa0253220:intron 35.0%
GAAGAAAGAATGATGTGCAA+TGG - chr2_2:44860601-44860620 None:intergenic 35.0%
!!! GATATTACTTTTTGGACTGG+TGG + chr2_2:44860675-44860694 Msa0253220:CDS 35.0%
! ATTTTTGTGCAGGATTTTGC+GGG + chr2_2:44860991-44861010 Msa0253220:intron 35.0%
GAATTACCGTGCAAATACTT+GGG - chr2_2:44861092-44861111 None:intergenic 35.0%
CAAGTATTTGCACGGTAATT+CGG + chr2_2:44861091-44861110 Msa0253220:intron 35.0%
! AAAGTTTTTGCCAATGCATG+AGG + chr2_2:44861225-44861244 Msa0253220:CDS 35.0%
! CATCTTTTGTAGGTAATGCA+GGG - chr2_2:44861257-44861276 None:intergenic 35.0%
CTTGCAATTTGATGCAACAT+AGG + chr2_2:44861281-44861300 Msa0253220:three_prime_UTR 35.0%
!!! AGTGGATTGGTTGTTCGTTT+TGG + chr2_2:44860095-44860114 Msa0253220:CDS 40.0%
!! AATGGATTTTGTGCCAACAG+TGG + chr2_2:44860127-44860146 Msa0253220:CDS 40.0%
! ATGGATTTTGTGCCAACAGT+GGG + chr2_2:44860128-44860147 Msa0253220:CDS 40.0%
TTCATCTGCATCTAGAAGCA+AGG + chr2_2:44860241-44860260 Msa0253220:CDS 40.0%
GCAAACTCAGGATACTTGAA+TGG - chr2_2:44860426-44860445 None:intergenic 40.0%
AATTGTTACTGAAGGGTCAG+AGG + chr2_2:44860740-44860759 Msa0253220:CDS 40.0%
ATTGTTACTGAAGGGTCAGA+GGG + chr2_2:44860741-44860760 Msa0253220:CDS 40.0%
! GGGCTTTATATATCACACCA+AGG + chr2_2:44860761-44860780 Msa0253220:CDS 40.0%
GCAGGTTGTAAAACTTGAGA+CGG + chr2_2:44860908-44860927 Msa0253220:intron 40.0%
CAGGTTGTAAAACTTGAGAC+GGG + chr2_2:44860909-44860928 Msa0253220:intron 40.0%
! ATTTTGCGGGCAGTATTAGA+GGG + chr2_2:44861004-44861023 Msa0253220:CDS 40.0%
TTATAGGATTCTCTACAGCG+TGG + chr2_2:44861059-44861078 Msa0253220:CDS 40.0%
TTCTGACCCAAGTATTTGCA+CGG + chr2_2:44861083-44861102 Msa0253220:CDS 40.0%
CGAATTACCGTGCAAATACT+TGG - chr2_2:44861093-44861112 None:intergenic 40.0%
! GCATCTTTTGTAGGTAATGC+AGG - chr2_2:44861258-44861277 None:intergenic 40.0%
! TGCAAGACAGCATCTTTTGT+AGG - chr2_2:44861267-44861286 None:intergenic 40.0%
TAAATGCCACCCCATTAAGT+GGG - chr2_2:44861519-44861538 None:intergenic 40.0%
!!! AGTGGATTGGTTGTTCGTTT+TGG + chr2_2:44860095-44860114 Msa0253220:CDS 40.0%
!! AATGGATTTTGTGCCAACAG+TGG + chr2_2:44860127-44860146 Msa0253220:CDS 40.0%
! ATGGATTTTGTGCCAACAGT+GGG + chr2_2:44860128-44860147 Msa0253220:CDS 40.0%
TTCATCTGCATCTAGAAGCA+AGG + chr2_2:44860241-44860260 Msa0253220:CDS 40.0%
GCAAACTCAGGATACTTGAA+TGG - chr2_2:44860426-44860445 None:intergenic 40.0%
AATTGTTACTGAAGGGTCAG+AGG + chr2_2:44860740-44860759 Msa0253220:CDS 40.0%
ATTGTTACTGAAGGGTCAGA+GGG + chr2_2:44860741-44860760 Msa0253220:CDS 40.0%
! GGGCTTTATATATCACACCA+AGG + chr2_2:44860761-44860780 Msa0253220:CDS 40.0%
GCAGGTTGTAAAACTTGAGA+CGG + chr2_2:44860908-44860927 Msa0253220:intron 40.0%
CAGGTTGTAAAACTTGAGAC+GGG + chr2_2:44860909-44860928 Msa0253220:intron 40.0%
! ATTTTGCGGGCAGTATTAGA+GGG + chr2_2:44861004-44861023 Msa0253220:CDS 40.0%
TTATAGGATTCTCTACAGCG+TGG + chr2_2:44861059-44861078 Msa0253220:CDS 40.0%
TTCTGACCCAAGTATTTGCA+CGG + chr2_2:44861083-44861102 Msa0253220:CDS 40.0%
CGAATTACCGTGCAAATACT+TGG - chr2_2:44861093-44861112 None:intergenic 40.0%
! GCATCTTTTGTAGGTAATGC+AGG - chr2_2:44861258-44861277 None:intergenic 40.0%
! TGCAAGACAGCATCTTTTGT+AGG - chr2_2:44861267-44861286 None:intergenic 40.0%
TAAATGCCACCCCATTAAGT+GGG - chr2_2:44861519-44861538 None:intergenic 40.0%
TTTCTTGAAAGCAGGCGCAT+CGG - chr2_2:44860168-44860187 None:intergenic 45.0%
AGGGTCTTCAGCAACTTTCA+GGG - chr2_2:44860451-44860470 None:intergenic 45.0%
TGACTTTGGCAAGCTTACCT+TGG - chr2_2:44860781-44860800 None:intergenic 45.0%
GACACTCAATGACGAATAGC+AGG + chr2_2:44860890-44860909 Msa0253220:intron 45.0%
! GATTTTGCGGGCAGTATTAG+AGG + chr2_2:44861003-44861022 Msa0253220:CDS 45.0%
! TTTTGCGGGCAGTATTAGAG+GGG + chr2_2:44861005-44861024 Msa0253220:CDS 45.0%
ATTAGAGGGGTTCACACTAC+TGG + chr2_2:44861018-44861037 Msa0253220:CDS 45.0%
TCTTGCCACACCCACTTAAT+GGG + chr2_2:44861506-44861525 Msa0253220:three_prime_UTR 45.0%
GTAAATGCCACCCCATTAAG+TGG - chr2_2:44861520-44861539 None:intergenic 45.0%
TTTCTTGAAAGCAGGCGCAT+CGG - chr2_2:44860168-44860187 None:intergenic 45.0%
AGGGTCTTCAGCAACTTTCA+GGG - chr2_2:44860451-44860470 None:intergenic 45.0%
TGACTTTGGCAAGCTTACCT+TGG - chr2_2:44860781-44860800 None:intergenic 45.0%
GACACTCAATGACGAATAGC+AGG + chr2_2:44860890-44860909 Msa0253220:intron 45.0%
! GATTTTGCGGGCAGTATTAG+AGG + chr2_2:44861003-44861022 Msa0253220:CDS 45.0%
! TTTTGCGGGCAGTATTAGAG+GGG + chr2_2:44861005-44861024 Msa0253220:CDS 45.0%
ATTAGAGGGGTTCACACTAC+TGG + chr2_2:44861018-44861037 Msa0253220:CDS 45.0%
TCTTGCCACACCCACTTAAT+GGG + chr2_2:44861506-44861525 Msa0253220:three_prime_UTR 45.0%
GTAAATGCCACCCCATTAAG+TGG - chr2_2:44861520-44861539 None:intergenic 45.0%
!! ATTAAATTAAATTATTATAC+AGG - chr2_2:44860350-44860369 None:intergenic 5.0%
!! ATTAAATTAAATTATTATAC+AGG - chr2_2:44860350-44860369 None:intergenic 5.0%
CCAGGAGCTTTCTTGAAAGC+AGG - chr2_2:44860176-44860195 None:intergenic 50.0%
CCTGCTTTCAAGAAAGCTCC+TGG + chr2_2:44860173-44860192 Msa0253220:CDS 50.0%
GCTTTCAAGAAAGCTCCTGG+TGG + chr2_2:44860176-44860195 Msa0253220:CDS 50.0%
ACTTTCAGGGCAGCAAACTC+AGG - chr2_2:44860438-44860457 None:intergenic 50.0%
CAGGGTCTTCAGCAACTTTC+AGG - chr2_2:44860452-44860471 None:intergenic 50.0%
CTGAAAGTTGCTGAAGACCC+TGG + chr2_2:44860450-44860469 Msa0253220:intron 50.0%
AAAGTTGCTGAAGACCCTGG+TGG + chr2_2:44860453-44860472 Msa0253220:intron 50.0%
! CTTGAGACGGGTTTGCTTTG+AGG + chr2_2:44860921-44860940 Msa0253220:intron 50.0%
AGGGATTGCTCCTCATGCAT+TGG - chr2_2:44861238-44861257 None:intergenic 50.0%
CTCTTGCCACACCCACTTAA+TGG + chr2_2:44861505-44861524 Msa0253220:three_prime_UTR 50.0%
CTTGCCACACCCACTTAATG+GGG + chr2_2:44861507-44861526 Msa0253220:three_prime_UTR 50.0%
CCAGGAGCTTTCTTGAAAGC+AGG - chr2_2:44860176-44860195 None:intergenic 50.0%
CCTGCTTTCAAGAAAGCTCC+TGG + chr2_2:44860173-44860192 Msa0253220:CDS 50.0%
GCTTTCAAGAAAGCTCCTGG+TGG + chr2_2:44860176-44860195 Msa0253220:CDS 50.0%
ACTTTCAGGGCAGCAAACTC+AGG - chr2_2:44860438-44860457 None:intergenic 50.0%
CAGGGTCTTCAGCAACTTTC+AGG - chr2_2:44860452-44860471 None:intergenic 50.0%
CTGAAAGTTGCTGAAGACCC+TGG + chr2_2:44860450-44860469 Msa0253220:intron 50.0%
AAAGTTGCTGAAGACCCTGG+TGG + chr2_2:44860453-44860472 Msa0253220:intron 50.0%
! CTTGAGACGGGTTTGCTTTG+AGG + chr2_2:44860921-44860940 Msa0253220:intron 50.0%
AGGGATTGCTCCTCATGCAT+TGG - chr2_2:44861238-44861257 None:intergenic 50.0%
CTCTTGCCACACCCACTTAA+TGG + chr2_2:44861505-44861524 Msa0253220:three_prime_UTR 50.0%
CTTGCCACACCCACTTAATG+GGG + chr2_2:44861507-44861526 Msa0253220:three_prime_UTR 50.0%
AGAGACACTGCACCCACTGT+TGG - chr2_2:44860143-44860162 None:intergenic 55.0%
AGACCCTGGTGGCATTCTCA+AGG + chr2_2:44860464-44860483 Msa0253220:intron 55.0%
! TGGCCTTGAGAATGCCACCA+GGG - chr2_2:44860470-44860489 None:intergenic 55.0%
!! TTGGCCTTGAGAATGCCACC+AGG - chr2_2:44860471-44860490 None:intergenic 55.0%
TGGTGGCATTCTCAAGGCCA+AGG + chr2_2:44860470-44860489 Msa0253220:intron 55.0%
GGGGTTCACACTACTGGTGA+TGG + chr2_2:44861024-44861043 Msa0253220:CDS 55.0%
AGAGACACTGCACCCACTGT+TGG - chr2_2:44860143-44860162 None:intergenic 55.0%
AGACCCTGGTGGCATTCTCA+AGG + chr2_2:44860464-44860483 Msa0253220:intron 55.0%
! TGGCCTTGAGAATGCCACCA+GGG - chr2_2:44860470-44860489 None:intergenic 55.0%
!! TTGGCCTTGAGAATGCCACC+AGG - chr2_2:44860471-44860490 None:intergenic 55.0%
TGGTGGCATTCTCAAGGCCA+AGG + chr2_2:44860470-44860489 Msa0253220:intron 55.0%
GGGGTTCACACTACTGGTGA+TGG + chr2_2:44861024-44861043 Msa0253220:CDS 55.0%
!! ACGTTTGCAGGAGCACCACC+AGG - chr2_2:44860194-44860213 None:intergenic 60.0%
! TGGTGGTGCTCCTGCAAACG+TGG + chr2_2:44860193-44860212 Msa0253220:CDS 60.0%
AAGCCACCTGCCACGTTTGC+AGG - chr2_2:44860206-44860225 None:intergenic 60.0%
GGCAGGTGGCTTCTCTGAGT+CGG + chr2_2:44860214-44860233 Msa0253220:CDS 60.0%
GCAGGTGGCTTCTCTGAGTC+GGG + chr2_2:44860215-44860234 Msa0253220:CDS 60.0%
CAGGTGGCTTCTCTGAGTCG+GGG + chr2_2:44860216-44860235 Msa0253220:CDS 60.0%
AGGTGGCTTCTCTGAGTCGG+GGG + chr2_2:44860217-44860236 Msa0253220:CDS 60.0%
GCCACACCCACTTAATGGGG+TGG + chr2_2:44861510-44861529 Msa0253220:three_prime_UTR 60.0%
GCCACCCCATTAAGTGGGTG+TGG - chr2_2:44861514-44861533 None:intergenic 60.0%
!! ACGTTTGCAGGAGCACCACC+AGG - chr2_2:44860194-44860213 None:intergenic 60.0%
! TGGTGGTGCTCCTGCAAACG+TGG + chr2_2:44860193-44860212 Msa0253220:CDS 60.0%
AAGCCACCTGCCACGTTTGC+AGG - chr2_2:44860206-44860225 None:intergenic 60.0%
GGCAGGTGGCTTCTCTGAGT+CGG + chr2_2:44860214-44860233 Msa0253220:CDS 60.0%
GCAGGTGGCTTCTCTGAGTC+GGG + chr2_2:44860215-44860234 Msa0253220:CDS 60.0%
CAGGTGGCTTCTCTGAGTCG+GGG + chr2_2:44860216-44860235 Msa0253220:CDS 60.0%
AGGTGGCTTCTCTGAGTCGG+GGG + chr2_2:44860217-44860236 Msa0253220:CDS 60.0%
GCCACACCCACTTAATGGGG+TGG + chr2_2:44861510-44861529 Msa0253220:three_prime_UTR 60.0%
GCCACCCCATTAAGTGGGTG+TGG - chr2_2:44861514-44861533 None:intergenic 60.0%
GGTGCTCCTGCAAACGTGGC+AGG + chr2_2:44860197-44860216 Msa0253220:CDS 65.0%
GCTCCTGCAAACGTGGCAGG+TGG + chr2_2:44860200-44860219 Msa0253220:CDS 65.0%
GGTGGCTTCTCTGAGTCGGG+GGG + chr2_2:44860218-44860237 Msa0253220:CDS 65.0%
GGTGCTCCTGCAAACGTGGC+AGG + chr2_2:44860197-44860216 Msa0253220:CDS 65.0%
GCTCCTGCAAACGTGGCAGG+TGG + chr2_2:44860200-44860219 Msa0253220:CDS 65.0%
GGTGGCTTCTCTGAGTCGGG+GGG + chr2_2:44860218-44860237 Msa0253220:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 44860083 44861560 44860083 ID=Msa0253220;Name=Msa0253220
chr2_2 mRNA 44860083 44861560 44860083 ID=Msa0253220-mRNA-1;Parent=Msa0253220;Name=Msa0253220-mRNA-1;_AED=0.19;_eAED=0.22;_QI=0|0.66|0.75|1|1|0.75|4|315|150
chr2_2 exon 44860083 44860262 44860083 ID=Msa0253220-mRNA-1:exon:13788;Parent=Msa0253220-mRNA-1
chr2_2 exon 44860663 44860782 44860663 ID=Msa0253220-mRNA-1:exon:13789;Parent=Msa0253220-mRNA-1
chr2_2 exon 44861003 44861104 44861003 ID=Msa0253220-mRNA-1:exon:13790;Parent=Msa0253220-mRNA-1
chr2_2 exon 44861195 44861560 44861195 ID=Msa0253220-mRNA-1:exon:13791;Parent=Msa0253220-mRNA-1
chr2_2 CDS 44860083 44860262 44860083 ID=Msa0253220-mRNA-1:cds;Parent=Msa0253220-mRNA-1
chr2_2 CDS 44860663 44860782 44860663 ID=Msa0253220-mRNA-1:cds;Parent=Msa0253220-mRNA-1
chr2_2 CDS 44861003 44861104 44861003 ID=Msa0253220-mRNA-1:cds;Parent=Msa0253220-mRNA-1
chr2_2 CDS 44861195 44861245 44861195 ID=Msa0253220-mRNA-1:cds;Parent=Msa0253220-mRNA-1
chr2_2 three_prime_UTR 44861246 44861560 44861246 ID=Msa0253220-mRNA-1:three_prime_utr;Parent=Msa0253220-mRNA-1
Gene Sequence

>Msa0253220

TGCAAAGAAACAAGTGGATTGGTTGTTCGTTTTGGTAAAATGTTAATGGATTTTGTGCCAACAGTGGGTGCAGTGTCTCTAGCCGATGCGCCTGCTTTCAAGAAAGCTCCTGGTGGTGCTCCTGCAAACGTGGCAGGTGGCTTCTCTGAGTCGGGGGGTTCATCTGCATCTAGAAGCAAGATCAGTAAAGATGATATTACTTTTTGGACTGGTGGTGATGTATTAAACATGCTTATTCACCCTAAAATCATGCTTCTAATTGTTACTGAAGGGTCAGAGGGCTTTATATATCACACCAAGGATTTTGCGGGCAGTATTAGAGGGGTTCACACTACTGGTGATGGTGATGCATTTAATTATAGGATTCTCTACAGCGTGGCTTCTGACCCAAGTATTTGCACGAATCAGAAACGTCTCCGAGAAGTTTTCAAAAAAGTTTTTGCCAATGCATGA
Protein sequence

>Msa0253220

CKETSGLVVRFGKMLMDFVPTVGAVSLADAPAFKKAPGGAPANVAGGFSESGGSSASRSKISKDDITFWTGGDVLNMLIHPKIMLLIVTEGSEGFIYHTKDFAGSIRGVHTTGDGDAFNYRILYSVASDPSICTNQKRLREVFKKVFANA*