Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0265680 | AES75857.2 | 61.972 | 71 | 5 | 1 | 1 | 71 | 810 | 858 | 6.73e-14 | 75.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0265680 | G7KNB4 | 61.972 | 71 | 5 | 1 | 1 | 71 | 810 | 858 | 3.21e-14 | 75.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003890 | Msa0265680 | 0.803409 | 3.535070e-49 | -8.615850e-47 |
| Msa0265670 | Msa0265680 | 0.882320 | 1.150467e-70 | -8.615850e-47 |
| Msa0265680 | Msa0303590 | -0.818173 | 2.251126e-52 | -8.615850e-47 |
| Msa0265680 | Msa0303710 | 0.977223 | 2.357009e-143 | -8.615850e-47 |
| Msa0265680 | Msa0607910 | 0.812291 | 4.565659e-51 | -8.615850e-47 |
| Msa0265680 | Msa0672570 | 0.801307 | 9.578608e-49 | -8.615850e-47 |
| Msa0265680 | Msa0690860 | 0.821258 | 4.442522e-53 | -8.615850e-47 |
| Msa0265680 | Msa1108760 | 0.801857 | 7.388717e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCAAGAAACGACGAACTA+TGG | 0.243423 | 2_2:-66026863 | None:intergenic |
| TCCCATAGTTCGTCGTTTCT+TGG | 0.250866 | 2_2:+66026861 | Msa0265680:CDS |
| GATGTGCCCAACCTTCCTTC+TGG | 0.379370 | 2_2:-66026638 | None:intergenic |
| ATCTAGATCAGTTGAAGGTT+TGG | 0.389443 | 2_2:+66026658 | Msa0265680:CDS |
| TGTTTAGGCTAGTCAGTTAT+CGG | 0.390169 | 2_2:+66026770 | Msa0265680:intron |
| GGCATTCCAGAAGGAAGGTT+GGG | 0.443657 | 2_2:+66026632 | Msa0265680:CDS |
| TCTGCGGCGGCGTCTGAGTT+CGG | 0.453289 | 2_2:+66026893 | Msa0265680:CDS |
| TTGGAGGTCGTCAATGGCTT+GGG | 0.454685 | 2_2:+66026601 | None:intergenic |
| ATTGGAGGTCGTCAATGGCT+TGG | 0.460063 | 2_2:+66026600 | None:intergenic |
| GGCACATCTAGATCAGTTGA+AGG | 0.503288 | 2_2:+66026653 | Msa0265680:CDS |
| TCAATCTAGTGCTGATCAGT+TGG | 0.504193 | 2_2:+66026829 | Msa0265680:CDS |
| TGGCATTCCAGAAGGAAGGT+TGG | 0.534110 | 2_2:+66026631 | Msa0265680:CDS |
| ACCAAGAAACGACGAACTAT+GGG | 0.534141 | 2_2:-66026862 | None:intergenic |
| TCAATGGCTTGGGATATCAT+TGG | 0.557764 | 2_2:+66026611 | None:intergenic |
| TCATTGGCATTCCAGAAGGA+AGG | 0.583501 | 2_2:+66026627 | Msa0265680:CDS |
| GATATCATTGGCATTCCAGA+AGG | 0.591588 | 2_2:+66026623 | Msa0265680:CDS |
| AGTCAGTTATCGGAATCGCT+TGG | 0.610681 | 2_2:+66026780 | Msa0265680:CDS |
| TATCGGAATCGCTTGGTGTG+TGG | 0.640747 | 2_2:+66026787 | Msa0265680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTTTACTTGCTGATTA+AGG | + | chr2_2:66026713-66026732 | Msa0265680:intron | 25.0% |
| !!! | GTTTTTTACTTGCTGATTAA+GGG | + | chr2_2:66026714-66026733 | Msa0265680:intron | 25.0% |
| TTCAGATTATGTTCGCAATA+TGG | + | chr2_2:66026684-66026703 | Msa0265680:intron | 30.0% | |
| !!! | TTCTTGGTTTTAAGTTTCTG+CGG | + | chr2_2:66026877-66026896 | Msa0265680:CDS | 30.0% |
| ATCTAGATCAGTTGAAGGTT+TGG | + | chr2_2:66026658-66026677 | Msa0265680:CDS | 35.0% | |
| !! | GTTATTAGCCTTTCAAGCTT+CGG | + | chr2_2:66026741-66026760 | Msa0265680:intron | 35.0% |
| ! | TGTTTAGGCTAGTCAGTTAT+CGG | + | chr2_2:66026770-66026789 | Msa0265680:intron | 35.0% |
| GATATCATTGGCATTCCAGA+AGG | + | chr2_2:66026623-66026642 | Msa0265680:CDS | 40.0% | |
| !! | TCAATCTAGTGCTGATCAGT+TGG | + | chr2_2:66026829-66026848 | Msa0265680:CDS | 40.0% |
| ACCAAGAAACGACGAACTAT+GGG | - | chr2_2:66026865-66026884 | None:intergenic | 40.0% | |
| AACCAAGAAACGACGAACTA+TGG | - | chr2_2:66026866-66026885 | None:intergenic | 40.0% | |
| !!! | TTGGTTTTAAGTTTCTGCGG+CGG | + | chr2_2:66026880-66026899 | Msa0265680:CDS | 40.0% |
| TCATTGGCATTCCAGAAGGA+AGG | + | chr2_2:66026627-66026646 | Msa0265680:CDS | 45.0% | |
| GGCACATCTAGATCAGTTGA+AGG | + | chr2_2:66026653-66026672 | Msa0265680:CDS | 45.0% | |
| AGTCAGTTATCGGAATCGCT+TGG | + | chr2_2:66026780-66026799 | Msa0265680:CDS | 45.0% | |
| TCCCATAGTTCGTCGTTTCT+TGG | + | chr2_2:66026861-66026880 | Msa0265680:CDS | 45.0% | |
| TGGCATTCCAGAAGGAAGGT+TGG | + | chr2_2:66026631-66026650 | Msa0265680:CDS | 50.0% | |
| GGCATTCCAGAAGGAAGGTT+GGG | + | chr2_2:66026632-66026651 | Msa0265680:CDS | 50.0% | |
| !! | AAGCTTCGGTGTCGCTGTTT+AGG | + | chr2_2:66026755-66026774 | Msa0265680:intron | 50.0% |
| !! | TATCGGAATCGCTTGGTGTG+TGG | + | chr2_2:66026787-66026806 | Msa0265680:CDS | 50.0% |
| GATGTGCCCAACCTTCCTTC+TGG | - | chr2_2:66026641-66026660 | None:intergenic | 55.0% | |
| CAGCGACACCGAAGCTTGAA+AGG | - | chr2_2:66026752-66026771 | None:intergenic | 55.0% | |
| TCTGCGGCGGCGTCTGAGTT+CGG | + | chr2_2:66026893-66026912 | Msa0265680:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 66026614 | 66026923 | 66026614 | ID=Msa0265680;Name=Msa0265680 |
| chr2_2 | mRNA | 66026614 | 66026923 | 66026614 | ID=Msa0265680-mRNA-1;Parent=Msa0265680;Name=Msa0265680-mRNA-1;_AED=0.47;_eAED=0.62;_QI=0|0|0|1|1|0.5|2|0|71 |
| chr2_2 | exon | 66026614 | 66026679 | 66026614 | ID=Msa0265680-mRNA-1:exon:20861;Parent=Msa0265680-mRNA-1 |
| chr2_2 | exon | 66026777 | 66026923 | 66026777 | ID=Msa0265680-mRNA-1:exon:20862;Parent=Msa0265680-mRNA-1 |
| chr2_2 | CDS | 66026614 | 66026679 | 66026614 | ID=Msa0265680-mRNA-1:cds;Parent=Msa0265680-mRNA-1 |
| chr2_2 | CDS | 66026777 | 66026923 | 66026777 | ID=Msa0265680-mRNA-1:cds;Parent=Msa0265680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
